Manufacturing method of medicine with gene recombination functional enzyme

Abstract

The invention provides a manufacturing method of a medicine with a gene recombination functional enzyme, and relates to the technical field of enzyme manufacturing, and the manufacturing method comprises the following steps: S1, plant pretreatment: sorting, cleaning and drying plants to obtain hyperaccumulators required by manufacturing; according to the invention, a matched medicine filling device is adopted to realize full-automatic unattended operation to automatically complete the steps of large bag filling and sealing, automatic bag supply, automatic assembly filling, automatic vacuumizing and automatic bag sealing in the steps S4-S10, so that high-efficiency production, high-efficiency filling and high-efficiency bag sealing are automatically realized; sealing is conducted after vacuumizing is conducted, the storage space is compressed through vacuumizing, the situation that in the transferring and storing process, bag expansion and bag breaking are not prone to being caused by internal air pressure is facilitated, and the phenomena of high hot melting efficiency and high sealing speed are achieved in the bag sealing process in a bag strip guiding and rolling hot melting mode; the rolling hot melting mode can effectively solve the problem that the over-melting phenomenon is prone to occurring in the hot melting process, and the hot-melted bag opening is tight in sealing and not prone to degumming.

Description

Technical Field

[0001] The invention relates to the technical field of enzyme production, in particular to a method for producing a medicine with gene recombination functional enzymes. Background Art

[0002] Superoxide dismutase is a type of metal enzyme widely present in organisms that defends against oxidative damage and is a recognized efficient oxygen free radical scavenger. Studies have shown that scavenging oxygen free radicals has a very positive effect on maintaining a good life state in organisms. Therefore, superoxide dismutase has a high application value and market unit price, especially in the markets of medicine, health food and cosmetics. People have isolated SOD from a variety of organisms including bacteria, fungi, algae, plants and animals. However, there are potential safety issues in extracting SOD from animals, and it is banned by the European Union and other places. Therefore, the use of plant resources to prepare SOD is a path to explore. The crude extraction methods of SOD in the prior art mainly include: ultrasonic extraction, enzyme extraction, phosphate buffer extraction, Tris-HCl and thermal denaturation. The crude extract obtained by the above method contains a large amount of impurities. In order to purify SOD, precipitation methods are often used, such as thermal denaturation, acetone graded precipitation, ammonium sulfate salting out and chromatography, etc., by selectively precipitating impurities or selectively precipitating required components to improve the specific activity of the enzyme.

[0003] At present, there are also related patent technologies containing methods for producing and processing genetically recombinant antioxidant enzymes. For example, patent No. CN201810845277.2 discloses a method for preparing superoxide dismutase-enriched dry powder, in which superoxide dismutase is extracted from super-enriched plants. By selecting super-enriched plants as raw materials for extraction, not only a large amount of superoxide dismutase can be enriched, providing a richer and more stable source of superoxide dismutase, but also the problem of resource utilization of cut biomass waste in plant restoration projects is solved. Moreover, the method is universal for the extraction and enrichment of superoxide dismutase from typical super-enriched plants.

[0004] Although the above patent discloses a method for manufacturing a drug with a genetically recombinant functional enzyme, the actual implementation of the method for manufacturing a drug with a genetically recombinant functional enzyme still requires relevant hardware support, and the existing method for manufacturing and processing drugs containing genetically recombinant antioxidant enzymes still has some shortcomings, the specific shortcomings are: The prepared superoxide dismutase enriched dry powder needs to be sealed during transportation and storage. Currently, the existing ones are sealed in small packages, which have a small storage capacity and need to be frequently unpacked during the later drug production process, resulting in low production efficiency. Therefore, it is necessary to provide a new drug manufacturing method with genetically recombinant functional enzymes. Summary of the invention

[0005] In view of the problems in the prior art, the purpose of the present invention is to provide a method for manufacturing a drug with a genetically recombinant functional enzyme, so as to solve the technical problem described in the above background technology that the prepared superoxide dismutase enriched dry powder needs to be sealed during transportation and storage, and the existing ones are all sealed in small packages, and the storage capacity of the small packages is small. The small packages need to be frequently unpacked in the later drug production process, resulting in low production efficiency.

[0006] The technical problem to be solved by the present invention is achieved by the following technical solution: a method for manufacturing a drug having a gene recombination functional enzyme, the method for manufacturing a drug having a gene recombination functional enzyme mainly comprises the following steps: S1. Plant pretreatment: sorting, cleaning and drying the plants to obtain the required super-enriched plants; S2, preparing ultrafiltration concentrate: immersing the pretreated super-enriched plant in a buffer solution containing a metal chelating agent, then crushing and homogenizing, filtering the obtained homogenate, repeating the leaching and filtration at least once, and combining the filtrate; adding ammonium sulfate to the super-enriched plant treatment solution to be salted out, standing and centrifuging to obtain the supernatant; adding deionized water to the super-enriched plant treatment solution to be removed for dilution, and ultrafiltration until the filtrate is clear and colorless, and taking the retentate; ultrafiltration and concentration of the super-enriched plant treatment solution to be concentrated; S3, precipitation and drying: slowly adding acetone to the super-enriched plant treatment solution to be precipitated and enriched until the volume concentration of acetone in the solution reaches 60-70%, standing, then centrifuging to obtain a precipitate, removing acetone, and freeze-drying the superoxide dismutase-enriched precipitate obtained in the precipitation and enrichment step to prepare a superoxide dismutase-enriched dry powder; S4, powder transfer and bulk bag making: the prepared superoxide dismutase enriched dry powder is transferred to a powder storage tank for sealed filling, and the powder is quantitatively transported to a drug filling device through the powder storage tank; S5. Automatic picking of aluminum foil bags: set the automatic picking of aluminum foil bags to face the opening of the aluminum foil bags forward and place the aluminum foil bags flat on the bag feeding bracket, fix the aluminum foil bags with the bag opening bracket and open the opening of the aluminum foil bags; S6. Insert the opening of the aluminum foil bag into the bottom of the powder injection tube: a swing arm frame that swings back and forth vertically is set to pick up the opened opening of the aluminum foil bag and swing it vertically, so that the aluminum foil bag originally placed flat is swung to a vertical state, and the opening of the aluminum foil bag is always kept in an open state during the swinging process, and the opened opening of the aluminum foil bag is inserted into the outer wall of the powder injection tube at an inclined angle; S7, clamping the aluminum foil bag opening: a clamping device is set on the aluminum foil bag opening on the outer wall of the powder injection tube to tightly wrap the aluminum foil bag opening on the outer wall of the powder injection tube; S8, emptying rod leading, powder injection: In step S4, the powder storage tank quantitatively transports the powder to the powder injection tube, and the powder is sprayed into the aluminum foil bag wrapped on the outer wall of the powder injection tube through the powder injection tube. Before spraying the powder, the emptying rod is arranged to extend into the aluminum foil bag to open the aluminum foil bag with the inner wall attached together, and the powder injection tube injects a quantitative amount of powder into the aluminum foil bag; S9, vacuum compression: vacuum compression is performed on the inside of the aluminum foil bag filled with quantitative powder, and a vacuum suction rod is set to extend into the aluminum foil bag to extract the air in the aluminum foil bag. The vacuum suction rod slowly extends upwards during the process of extracting air from the aluminum foil bag, and cooperates with the clamping plate to clamp upwards in sections until the air in the aluminum foil bag is exhausted; S10, aluminum foil bag plastic sealing: the aluminum foil bag opening from which air has been exhausted is horizontally slid and hot-melt plastic sealed, and the aluminum foil bag opening is hot-melt plastic sealed and fastened by means of bag pressing wheel and hot-melt wheel rolling and hot-melt during the horizontal sliding process of the aluminum foil bag; S11. Bulk bag transfer: The hot-melt plastic-sealed aluminum foil bag is transferred to the next station by a robot for transportation or long-term storage; S12, mixing: putting the large bag of powder prepared in step S11 into a mixing device, adding drug components and mixing the powder until the mixture is uniform to obtain drug powder; S13, bagging and packaging: quantitatively divide the drug powder obtained in step S12 into small bags and seal them to obtain finished drugs.

[0007] As a preferred technical solution of the present invention, the above-mentioned steps S4-S10 are mainly completed by a drug filling device, which includes a bottom chassis arranged on the bottom surface, and a top frame arranged in the middle of the house, wherein the bottom of the top frame in the middle of the house is provided with an automatic bag supply assembly, a filling assembly, a vacuum assembly, and a bag sealing assembly; the filling assembly, the vacuum assembly, and the bag sealing assembly are arranged in a row at the bottom of the top frame, The bottom chassis are provided with two at the bottom of the top frame, and an intermittently rotating conveyor belt is provided in the middle of the two bottom chassis. A push rod frame 1 is provided in the middle of the two bottom chassis at the bottom of the filling component, and a reciprocating lifting tray is provided at the top of the push rod frame 1. A slide groove is provided at the top of each of the two bottom chassis, and a sliding frame is slidably connected to the inside of each slide groove, and a transmission screw is rotatably connected to the inside of the slide groove. The sliding frame slidably connected to the transmission screw in the slide groove and the transmission screw Screw transmission, a clamping cylinder 1 is provided in the middle of the sliding frame on each side, and a clamping plate is extended toward the middle direction of the bottom chassis from the clamping cylinder 1 on both sides, and a clamping cylinder 2 is provided at the top of the sliding frame on each side, and a lifting cylinder is fixedly installed on the top of the sliding frame on each side, and a push rod is extended upward from the lifting cylinder, and a clamping cylinder 3 is fixedly installed on the top of the push rod of the lifting cylinder, and a clamping plate is also extended toward the middle direction of the bottom chassis from the clamping cylinder 3 and the clamping cylinder 2.

[0008] As a preferred technical solution of the present invention, the automatic bag supply assembly is arranged next to the filling assembly, and the automatic bag supply assembly includes a bag lifting cylinder fixedly installed at the rear end of the top frame, a piston rod extending from the bottom of the bag lifting cylinder, a bag grabbing suction cup is arranged at the bottom of the piston rod of the bag lifting cylinder, and two intermittently circulating chains are arranged below the bag lifting cylinder, a bag feeding bracket is arranged at an equal interval between the two circulating chains, and a spacing is arranged between two adjacent sections of the bag feeding brackets, and an aluminum foil bag stacking box is arranged between the two circulating chains, and a plurality of aluminum foil bags with the bag openings facing the same direction and neatly stacked are placed on the top of the aluminum foil bag stacking box.

[0009] As a preferred technical solution of the present invention, the bag lifting cylinder is located on one side of the intermittently rotating chain, and a bag lifting cylinder is arranged above the other side of the intermittently rotating chain. A piston rod extends downward from the bottom of the bag lifting cylinder, and a horizontally placed bag lifting bracket is fixed to the bottom end of the piston rod of the bag lifting cylinder. The bag lifting bracket is arranged in the middle of the two intermittently rotating chains, and the bag lifting bracket is parallel to the two intermittently rotating chains. A bag lifting suction cup is arranged at the bottom of the bag lifting bracket.

[0010] As a preferred technical solution of the present invention, the middle part of the top frame is rotatably connected with a rotating shaft, the outer wall of the rotating shaft is fixedly installed with a swing arm frame, the swing arm frame includes two rack slide grooves arranged on the outer wall of the swing arm frame, the inner walls of the two rack slide grooves are slidably connected with second racks, and the middle position of the two second racks is rotatably connected with a synchronous gear, the end of one of the second racks is provided with a first clamping plate, and the end of the other second rack is provided with a second clamping plate, and the first clamping plate and the second clamping plate are attached to each other and separated from each other; The swing arm frame also includes two hinge supports symmetrically arranged on both sides of the outer wall of the swing arm frame, each of the hinge supports is rotatably connected to a swing arm strut on the surface, each of the swing arm struts is an arc-shaped rod, and a support opening sheet is arranged on the top of each swing arm strut, and the support opening sheet is a thin wide iron sheet, and an arc-shaped slide groove is opened in the middle of the swing arm strut, and a swing arm cylinder is rotatably connected to the surface of the swing arm frame, and a piston rod is extended upward from the swing arm cylinder, and the top of the piston rod of the swing arm cylinder is slidably connected in the arc-shaped slide groove.

[0011] As a preferred technical solution of the present invention, the filling assembly includes a powder injection tube arranged at the bottom of the top frame, the powder injection tube is a circular tube structure with a hollow interior, a powder injection rod is fixedly installed inside the powder injection tube, the bottom end of the powder injection rod slightly extends toward the bottom of the powder injection tube, and emptying columns are symmetrically arranged on the left and right sides of the powder injection rod, and the emptying columns on both sides can slide up and down on the surface of the powder injection rod and extend out, and the top ends of the emptying columns on both sides are fixed on the lifting frame 1, and the left and right sides of the lifting frame 1 are symmetrically arranged with a pneumatic cylinder 1; Two bag mouth clamps are symmetrically arranged on the front and rear side walls of the powder injection tube, each of the bag mouth clamps is an arc structure, and the outer wall of each bag mouth clamp is provided with a clamp support rod, and the other end of the clamp support rod is rotatably hinged to the surface of the powder injection tube. Two push rod cylinders are symmetrically arranged on the front and rear side walls of the powder injection tube, and the top end of the piston rod of each push rod cylinder is rotatably connected to the surface of the clamp support rod.

[0012] As a preferred technical solution of the present invention, double-push cylinders are symmetrically arranged on the left and right sides of the powder injection tube, wherein a spacing is arranged between the double-push cylinders on each side and the outer wall of the powder injection tube, a bottom fixed support rod is fixedly installed on the top of the double-push cylinders on each side, and the top of the bottom fixed support rod on each side is fixed to the top of the top frame, piston rods are symmetrically extended from the front and rear sides of the double-push cylinders on each side, and a bag-mouth side clamp is arranged on the top of the piston rod on the front and rear sides of the double-push cylinders on each side.

[0013] As a preferred technical solution of the present invention, the bag sealing assembly includes a suspended plastic sealing box arranged at the bottom of the top frame, the outer wall of the suspended plastic sealing box is provided with a supporting frame, and the supporting frame is fixed to the bottom of the top frame, and a sealing groove for the aluminum foil bag to pass through is provided in the middle of the inner wall of the bottom surface of the suspended plastic sealing box, and the inner wall of the sealing groove is symmetrically provided with circulating pressing strips on the front and back sides, and the two pressing strips are provided with two on each side of the inner wall of the sealing groove, and the two pressing strips rotate in parallel, and the surface of each pressing strip is flat, and a hot melt wheel is provided in the middle position of the two pressing strips on one side of the sealing groove, and a pressing wheel is provided in the middle position of the two pressing strips on the other side of the sealing groove, and an arc-shaped concave hot melt groove is provided in the middle position of the outer cylindrical surface of the hot melt wheel, and an extrusion ring matching the hot melt groove is provided on the outer cylindrical surface of the pressing strip rotating wheel.

[0014] As a preferred technical solution of the present invention, hot melt wheel shafts are symmetrically arranged at both ends of the hot melt wheel, shaft sleeves are symmetrically arranged at both ends of the inner ring of the suspended plastic packaging chassis, two electrode terminals of positive and negative poles are arranged at one of the top ends of the hot melt wheel shaft, one of the electrode terminals is a circular ring structure, and the other electrode terminal is a circle center structure, and the inner wall of the shaft sleeve is provided with conductive terminals matching the two electrode terminals.

[0015] As a preferred technical solution of the present invention, the vacuum assembly includes a lifting frame 2 arranged at the top of the top frame, two vacuum suction rods are arranged at the bottom end of the lifting frame 2, and a pneumatic cylinder 2 is respectively arranged on the left and right sides of the lifting frame 2; Two clamping cylinders are arranged at the bottom end of the top frame, and piston rods are extended toward the middle of the two clamping cylinders. Clamping plates are arranged at the top ends of the piston rods of the two clamping cylinders, and each clamping plate fits the outer wall contour of the vacuum suction rod.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides a method for manufacturing a drug having a gene recombination functional enzyme, which can realize the packaging and sealing of superoxide dismutase-enriched dry powder, by storing and packaging the prepared superoxide dismutase-enriched dry powder in large sealed aluminum foil bags. The maximum amount of powder injected into the aluminum foil bag at one time is 25KG, which realizes sealed packaging of large bags. The large package is convenient for lifting and later transportation and storage, and the transportation speed is fast. At the same time, the powder of the large package can realize adding more powder to the powder storage tank at one time, reducing the tedious operation of frequent unpacking of small bags, and improving production efficiency. Among them, the present invention can realize fully automatic unattended automation to complete the large bag filling and sealing steps in steps S4-S10 by adopting a matching medicine filling device, automatic bag supply, automatic filling components, automatic vacuuming, and automatic bag sealing, and realize high-efficiency production, high-efficiency filling, and high-efficiency bag sealing automatically; vacuuming is adopted before sealing, and vacuuming compresses the storage space and is conducive to the transportation and storage process. It is not easy to cause the internal air pressure to expand the bag and break the bag. The storage process and the transportation process are very safe. The bag sealing process adopts the bag strip guidance and rolling hot melting method to achieve high hot melting efficiency and fast sealing speed. The rolling hot melting method can effectively solve the problem of over-melting during the hot melting process, and the bag mouth after hot melting is tightly sealed and not easy to debond.

[0017] The bag lifting cylinder drives the bag grabbing suction cup to descend downward, and the bag grabbing suction cup sucks the aluminum foil bag in the aluminum foil bag stacking box below, and lifts the aluminum foil bag at only one end of the aluminum foil bag, with the opening of the lifted aluminum foil bag facing downward, and drives a section of the bag feeding bracket to move horizontally forward from the rear side of the lifted aluminum foil bag through an intermittent cyclically rotating chain to push it forward. During the movement of the bag feeding bracket, it moves from the middle of the lifted aluminum foil bag toward the opening of the aluminum foil bag, so that the opening of the aluminum foil bag is horizontally laid forward on the top surface of a section of the bag feeding bracket. At this time, the bag grabbing suction cup is released, and the opening of the aluminum foil bag is horizontally conveyed forward to the bottom of the bag lifting cylinder. While a section of the bag feeding bracket moves forward to the bottom of the bag lifting cylinder, the bag lifting cylinder continues to push the bag grabbing suction cup to move downward to absorb the next aluminum foil bag. This step adopts a simple structural design, cooperates with the cyclic rotation of the chain, and utilizes the natural physical law to automatically realize the automatic picking and automatic spreading of the aluminum foil bag, and ensure that the direction of the bag opening is consistent during the spreading process. No manual supervision is required throughout the process, and automation improves production efficiency.

[0018] The aluminum foil bag is horizontally transported to the bottom of the bag lifting cylinder. The bag lifting bracket is pushed down by the bag lifting cylinder. The bag lifting bracket uses two bag lifting suction cups to respectively absorb the two ends of the aluminum foil bag. The top surface of the aluminum foil bag with the bag opening facing forward is gently lifted upward horizontally, and the bottom surface of the aluminum foil bag naturally droops on the surface of the bag feeding bracket by gravity, so that the aluminum foil bag opening and the aluminum foil bag belly are stretched open, and the swing arm frame is swung downward to a vertical state, and the opening thin sheet at the top of the swing arm support rod on the side of the swing arm frame is swung downward and extended into the aluminum foil bag opening, and the aluminum foil bag opening is stretched open by the width of the opening thin sheet. At this time, the bag lifting suction cup is released, and the first clamping plate and the second clamping plate on both sides of the swing arm frame symmetrically clamp the left and right sides of the aluminum foil bag opening respectively. , so that the two sides of the opened aluminum foil bag mouth are clamped, the middle of the aluminum foil bag mouth is opened in a mouth shape by two opening-opening thin sheets, and is swung to a horizontal state by the swing arm frame, and the aluminum foil bag is transported to the bottom of the powder injection tube. During the transportation process, since the swing arm frame is swung to a horizontal state, the aluminum foil bag mouth is opened by the width of the opening-opening thin sheets to maintain an open state, so that the aluminum foil bag mouth is inserted in an inclined direction during the initial contact with the bottom of the powder injection tube, and when the swing arm frame swings to a horizontal state, the aluminum foil bag mouth is gradually inserted into the bottom of the powder injection tube to a horizontal state. Such an operation can facilitate the aluminum foil bag mouth to be inserted into the bottom of the powder injection tube more smoothly, the insertion process is not easy to get stuck on the bottom surface of the powder injection tube, and the automated insertion process is more solid and reliable.

[0019] The aluminum foil bag mouth inserted into the bottom of the powder injection tube is symmetrically clamped from the front and rear sides of the outer wall of the powder injection tube by the bag mouth clamping plate of the outer wall of the powder injection tube. At this time, the swing arm support rod swings upward along the hinge support of the swing arm frame and is pulled out upward from the gap between the adjacent double-push cylinder and the powder injection tube. During the upward pulling process, the swing arm support rod will swing upward at a certain angle so that the support mouth thin sheet will not collide with the aluminum foil bag inserted into the bottom of the powder injection tube when the swing arm frame is retracted. The first clamping plate and the second clamping plate are clamped from the left and right sides of the aluminum foil bag mouth. The right two sides are loosened, and the swing arm frame retreats and swings to a vertical state, ready to pick up the next aluminum foil bag. When the bag mouth clamp clamps the aluminum foil bag on the outer wall of the powder filling tube, the left and right sides of the aluminum foil bag are clamped and sealed by the two bag mouth side clamps through the double push cylinders on both sides of the powder filling tube. Since each bag mouth side clamp is set horizontally, the left and right sides of the aluminum foil bag mouth are clamped horizontally, which increases the sealing of the aluminum foil bag mouth during powder filling and reduces the leakage of powder from the aluminum foil bag mouth.

[0020] In the process of injecting powder into the aluminum foil bag through the powder injection tube, the present invention first extends downward from the bottom of the powder injection tube through the emptying column, and the powder injection tube will extend into the middle of the aluminum foil bag to isolate and support the inner wall of the aluminum foil bag adsorbed together, which is beneficial to reducing the impact on the inner wall of the aluminum foil bag caused by the powder injection rod inside the powder injection tube during the process of spraying powder downward. By reducing the impact of the initial injection of powder into the aluminum foil bag, it is beneficial to reduce the phenomenon of powder floating in the aluminum foil bag. At the same time, it can further prevent the aluminum foil bag from swinging or shaking during the initial injection of powder, which is beneficial to smoother initial injection of powder into the aluminum foil bag. The emptying column is arranged inside the powder injection tube. During the powder injection process, as the amount of powder injected into the aluminum foil bag gradually increases, the emptying column gradually rises and is withdrawn from the powder injection tube. The emptying column is arranged inside the powder injection tube, which is beneficial to reducing the friction between the emptying column and the aluminum foil bag during the process of emptying the column, and can also increase the tightness of the aluminum foil bag opening during the powder injection process, effectively reducing the loss of powder from the aluminum foil bag opening.

[0021] After the powder filling inside the aluminum foil bag is completed, the sliding frame moves to the bottom of the filling assembly and lands on the top of the sliding frame through the clamping cylinder 3. At this time, the position of the clamping cylinder 3 at the top of the sliding frame is just above the aluminum foil bag. The clamping cylinders 3 on both sides push the aluminum foil bag back and forth three times from the upper position of the aluminum foil bag. With the tension of the aluminum foil bag mouth hanging on the powder filling tube, the upper half of the aluminum foil bag, which is hollow due to the lack of powder filling inside, is tightened. The clamping cylinders 3 on both sides push the aluminum foil bag back and forth three times from the upper position of the aluminum foil bag to shake the powder inside the aluminum foil bag and reduce the phenomenon of powder hanging on the inner wall of the aluminum foil bag. This effectively facilitates the later vacuum compression. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0023] Figure 1 The present invention is a flowchart of the steps of the method for producing a drug having a gene recombination functional enzyme; Figure 2 It is a front structural schematic diagram of the medicine filling device of the present invention; Figure 3 It is a left-side structural schematic diagram of the medicine filling device of the present invention; Figure 4 The left side structure diagram of the automatic bag supply assembly of the present invention is shown in FIG. Figure 1 ; Figure 5 The left side structure diagram of the automatic bag supply assembly of the present invention is shown in FIG. Figure 2 ; Figure 6 The left side structure diagram of the filling assembly of the present invention is shown in FIG. Figure 1 ; Figure 7 The left side structure diagram of the filling assembly of the present invention is shown in FIG. Figure 2 ; Figure 8 It is a left-side structural schematic diagram of the vacuum pumping assembly of the present invention; Fig. 9 It is a schematic diagram of a top cross-sectional structure of the bag sealing assembly of the present invention; Fig.10 It is a front cross-sectional structural schematic diagram of the sealing groove of the present invention; Fig.11 The present invention is attached to the specification Fig.10 A local enlarged view of point A; Fig.12 It is a schematic diagram of the structure of the electrode terminal at the top end of the hot melt rotary shaft of the present invention; Fig.13 It is a structural schematic diagram of the clamping plate of the present invention; In the figure: bottom chassis 1, conveyor belt 2, pulley 3, push rod frame 1 4, electric push rod 1 5, tray slot 6, tray 7, bag grabbing suction cup 8, powder injection tube 9, swing arm support rod 10, side clamping mechanism 11, top frame 12, lifting frame 1 13, powder injection rod 14, emptying column 15, pneumatic cylinder 1 16, lifting frame 2 17, vacuum suction rod 18, pneumatic cylinder 2 19, clamping plate 20, suspended plastic sealing chassis 21, bag pressing wheel 2101, hot melt wheel 2102, Bag pressing strip rotating wheel 2103, bag pressing strip 2104, hot melt groove 2105, rotating shaft sleeve 2106, hot melt wheel rotating shaft 2107, conductive terminal 2108, electrode terminal 2109, sealing groove 2110, extrusion ring 2111, support frame 22, slide groove 23, transmission screw 24, sliding frame 25, clamping cylinder 26, lifting cylinder 27, Clamping cylinder 28, clamping cylinder 39, arc chute 30, swing arm cylinder 31, hinge support 1 32, rotating shaft 32, swing arm frame 34, bag lifting cylinder 35, bag lifting bracket 36, bag lifting suction cup 37, bag feeding bracket 38, bag lifting cylinder 39, cylinder bracket 2 40, aluminum foil bag stacking box 41, chain 42, synchronous gear 43, second rack 44, rack chute 45, first clamping plate 46, second clamping plate 47, support plate 48, push rod cylinder 49, bottom fixed support rod 50, double push cylinder 51, clamping plate support rod 52, bag mouth side clamp 53, bag mouth clamp 54, clamping cylinder 55, clamping plate 56. DETAILED DESCRIPTION

[0024] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific diagrams. It should be noted that the embodiments in this application and the features in the embodiments can be combined with each other without conflict.

[0025] Example 1 See also Figure 1, which is a schematic diagram of the overall structure of a method for manufacturing a drug with a gene recombination functional enzyme, A method for producing a drug having a gene recombination functional enzyme, the method mainly comprising the following steps: S1. Plant pretreatment: sorting, cleaning and drying the plants to obtain the required super-enriched plants; S2, preparing ultrafiltration concentrate: immersing the pretreated super-enriched plant in a buffer solution containing a metal chelating agent, then crushing and homogenizing, filtering the obtained homogenate, repeating the leaching and filtration at least once, and combining the filtrate; adding ammonium sulfate to the super-enriched plant treatment solution to be salted out, standing and centrifuging to obtain the supernatant; adding deionized water to the super-enriched plant treatment solution to be removed for dilution, and ultrafiltration until the filtrate is clear and colorless, and taking the retentate; ultrafiltration and concentration of the super-enriched plant treatment solution to be concentrated; S3, precipitation and drying: slowly adding acetone to the super-enriched plant treatment solution to be precipitated and enriched until the volume concentration of acetone in the solution reaches 60-70%, standing, then centrifuging to obtain a precipitate, removing acetone, and freeze-drying the superoxide dismutase-enriched precipitate obtained in the precipitation and enrichment step to prepare a superoxide dismutase-enriched dry powder; S4, powder transfer and bulk bag making: the prepared superoxide dismutase enriched dry powder is transferred to a powder storage tank for sealed filling, and the powder is quantitatively transported to a drug filling device through the powder storage tank; S5, automatic picking of aluminum foil bag: setting the automatic picking of aluminum foil bag to be placed flat on the aluminum foil bag mouth feeding bracket 38, fixing the aluminum foil bag by the bag opening bracket and opening the aluminum foil bag; S6, inserting the opening of the aluminum foil bag into the bottom of the powder injection tube 9: a swing arm frame 34 is provided to swing vertically back and forth to pick up the opened opening of the aluminum foil bag and swing vertically, so as to swing the originally flat aluminum foil bag to a vertical state, and keep the opening of the aluminum foil bag in an open state during the swinging process, and the opened opening of the aluminum foil bag is inserted into the outer wall of the powder injection tube 9 at an inclined angle; S7, clamping the aluminum foil bag opening: a clamping device is set on the aluminum foil bag opening inserted into the outer wall of the powder injection tube 9 to tightly wrap the aluminum foil bag opening on the outer wall of the powder injection tube 9; S8, emptying rod leading, powder injection: In step S4, the powder storage tank quantitatively transports the powder to the powder injection tube 9, and the powder is sprayed into the aluminum foil bag wrapped on the outer wall of the powder injection tube 9 through the powder injection tube 9. Before spraying the powder, the emptying rod is first extended into the aluminum foil bag to open the aluminum foil bag with the inner wall attached together, and the powder injection tube 9 injects a quantitative amount of powder into the aluminum foil bag; S9, vacuum compression: vacuum compression is performed on the inside of the aluminum foil bag filled with quantitative powder, and a vacuum suction rod 18 is arranged to extend into the aluminum foil bag to extract the air in the aluminum foil bag. The vacuum suction rod 18 slowly extends upwards during the process of extracting air from the aluminum foil bag, and cooperates with the clamping plate 20 to clamp upwards in sections until the air in the aluminum foil bag is exhausted; S10, aluminum foil bag plastic sealing: the aluminum foil bag opening from which air has been exhausted is horizontally slid and hot-melt plastic sealed, and the aluminum foil bag opening is hot-melt plastic sealed and fastened by means of bag pressing wheel and hot-melt wheel 2102 during the horizontal sliding process of the aluminum foil bag; S11. Bulk bag transfer: The hot-melt plastic-sealed aluminum foil bag is transferred to the next station by a robot for transportation or long-term storage; S12, mixing: putting the large bag of powder prepared in step S11 into a mixing device, adding drug components and mixing the powder until the mixture is uniform to obtain drug powder; S13, bagging and packaging: quantitatively divide the drug powder obtained in step S12 into small bags and seal them to obtain finished drugs.

[0026] Among them, the drug manufacturing method with genetically recombinant functional enzyme provided by the present invention can realize the packaging and sealing of superoxide dismutase enriched dry powder, and the prepared superoxide dismutase enriched dry powder is stored and packaged in large sealed aluminum foil bags, which is convenient for later transportation and storage. At the same time, the large package of powder can be added to the powder storage tank at one time, reducing the cumbersome operation of frequent unpacking of small packages, and improving low production efficiency. Among them, the present invention can realize the full-automatic unattended automation of large package filling and sealing in steps S4-S10 by adopting a matching drug filling device. The sealing steps include automatic bag supply, automatic filling components, automatic vacuuming and automatic bag sealing, which can realize high-efficiency production, high-efficiency filling and high-efficiency bag sealing automatically. The system adopts vacuuming first and then sealing. Vacuuming compresses the storage space and is conducive to transportation. It is not easy to cause internal air pressure to swell and break the bag during storage. The storage and transportation processes are very safe. The bag sealing process adopts bag strip guidance and rolling hot melt to achieve high hot melt efficiency and fast sealing speed. The rolling hot melt method can effectively solve the problem of over-melting during the hot melt process, and the bag mouth after hot melt is tightly sealed and not easy to debond.

[0027] Example 2 See also Figure 2-Figure 13 This embodiment is similar to the above-mentioned embodiment 1, and the similarities are not described in this embodiment. The specific differences are: The steps S4-S10 described above are mainly completed by a drug filling device, which includes a bottom case 1 arranged on the bottom surface, and a top frame 12 arranged in the middle of the house, and an automatic bag supply component, a filling component, a vacuum component, and a bag sealing component are arranged at the bottom of the top frame 12 in the middle of the house; the filling component, the vacuum component, and the bag sealing component are arranged in a row at the bottom of the top frame 12, and there are two bottom cases 1 at the bottom of the top frame 12, and an intermittently rotating conveyor belt 2 is arranged in the middle of the two bottom cases 1, and the surface of the conveyor belt 2 is a plane, and the conveyor belt 2 A pulley 3 is provided at each end of the inner circle, and the two pulleys 3 are rotatably connected in the corresponding bottom chassis 1 respectively. One of the pulleys 3 is provided with an independent servo motor to drive the rotation. A push rod frame 4 is provided in the middle position of the two bottom chassis 1 at the bottom of the filling component. A reciprocating tray 7 is provided at the top of the push rod frame 4. A tray slot 6 is opened at the top of the push rod frame 4. An electric push rod 5 is fixedly installed at the bottom of the push rod frame 4. The electric push rod 5 passes through the tray slot 6 and a push rod extends to the top of the push rod frame 4. The bottom of the tray 7 is fixed to the top of the push rod of the electric push rod 5.

[0028] A slide slot 23 is provided at the top of each of the two bottom chassis 1. A sliding frame 25 is slidably connected inside each slide slot 23. A transmission screw 24 is rotatably connected inside the slide slot 23. One end of the transmission screw 24 located in the slide slot 23 is provided with an independent servo motor to drive rotation. The sliding frame 25 slidably connected in the slide slot 23 is threadedly driven by the transmission screw 24. A clamping cylinder 1 26 is provided in the middle of each side sliding frame 25. The clamping cylinders 1 26 on both sides each extend a clamping plate 20 toward the middle direction of the bottom chassis 1. A clamping cylinder 28 is provided at the top of each side sliding frame 25. A lifting cylinder 27 is fixedly installed at the top of each side sliding frame 25. A push rod extends upward from the lifting cylinder 27. A clamping cylinder 3 29 is fixedly installed at the top of the push rod of the lifting cylinder 27. The clamping cylinder 3 29 and the clamping cylinder 2 28 each extend a clamping plate 20 toward the middle direction of the bottom chassis 1. The filled aluminum foil bag is reciprocatedly transferred by the sliding rack 25 and transported to the bottom of the filling component, the vacuum component, and the bag sealing component in turn. When the transported aluminum foil bag completes the last step of bag sealing, the sliding rack 25 will automatically return to the bottom of the filling component and transfer another filled aluminum foil bag to the bottom of each component in turn.

[0029] The automatic bag supply assembly is arranged beside the filling assembly. The automatic bag supply assembly includes a bag lifting cylinder 39 fixedly installed at the rear end of the top frame 12. A piston rod extends from the bottom of the bag lifting cylinder 39. A bag grabbing suction cup 8 is arranged at the bottom of the piston rod of the bag lifting cylinder 39. Two intermittently circulating chains 42 are arranged below the bag lifting cylinder 35. A section of bag feeding bracket 38 is arranged at an equal interval between the two circulating chains 42. A distance is arranged between two adjacent sections of bag feeding bracket 38. An aluminum foil bag stacking box 41 is arranged between the two circulating chains 42. A plurality of aluminum foil bags with the bag openings facing the same direction and neatly stacked are placed on the top of the aluminum foil bag stacking box 41. The aluminum foil bag stacking box 41 is located in the middle of the two chains 42.

[0030] The bag lifting cylinder 35 is located on one side of the intermittently cyclically rotating chain 42. The inner ring of the intermittently cyclically rotating chain 42 is provided with a plurality of sprockets to support the transmission track of the chain, and the two chains are driven to rotate synchronously by an independent servo motor. The bag feeding bracket 38 is composed of a plurality of cylindrical rods with a certain spacing, which is convenient for feeding the aluminum foil bag. The bag lifting cylinder 35 is arranged above the other side of the intermittently cyclically rotating chain 42. A piston rod extends downward from the bottom of the bag lifting cylinder 35. A horizontally placed bag lifting bracket 36 is fixed to the bottom end of the piston rod of the bag lifting cylinder 35. The bag lifting bracket 36 is arranged in the middle of the two intermittently cyclically rotating chains 42, and the bag lifting bracket 36 is parallel to the two intermittently cyclically rotating chains 42. A bag lifting suction cup 37 is arranged at the bottom of the bag lifting bracket 36.

[0031] Specifically, in this embodiment, the present invention drives the bag grabbing suction cup 8 to descend downward through the bag lifting cylinder 35, and the bag grabbing suction cup 8 sucks the aluminum foil bag in the aluminum foil bag stacking box 41 below, and lifts the aluminum foil bag at only one end of the aluminum foil bag, and the opening of the lifted aluminum foil bag faces downward, and drives a section of the bag feeding bracket 38 to move horizontally forward from the rear side of the lifted aluminum foil bag through the intermittent cyclic rotating chain 42. During the movement of the bag feeding bracket 38, it moves from the middle of the lifted aluminum foil bag toward the opening of the aluminum foil bag, so that the opening of the aluminum foil bag faces forward and is horizontally laid on the top surface of a section of the bag feeding bracket 38. At this time, the bag grabbing suction cup 8 is released, and the aluminum foil bag opening is horizontally transported to the bottom of the bag lifting cylinder 35 with the opening facing forward. While a bag feeding bracket 38 moves forward to the bottom of the bag lifting cylinder 35, the bag lifting cylinder 39 continues to push the bag grabbing suction cup 8 to move downward to absorb the next aluminum foil bag. This step adopts a simple structural design, cooperates with the circular rotation of the chain 42, and utilizes the natural physical laws to automatically realize the automatic picking and automatic spreading of the aluminum foil bags, and ensures that the bag opening is in the same direction during the spreading process. No manual supervision is required throughout the process, and automation improves production efficiency.

[0032] The middle part of the top frame 12 is rotatably connected with a rotating shaft 32, one end of which is provided with an independent servo motor to drive the rotation, and the outer wall of the rotating shaft 32 is fixedly installed with a swing arm frame 34, and the swing arm frame 34 includes two rack slide grooves 45 provided on the outer wall of the swing arm frame 34, and the inner walls of the two rack slide grooves 45 are slidably connected with a second rack 44, and the middle position of the two second racks 44 is rotatably connected with a synchronous gear 43, and the synchronous gear 43 is provided with an independent servo motor to drive the rotation, and a first clamping plate 46 is provided at the end of one of the second racks 44, and a second clamping plate 47 is provided at the end of the other second rack 44, and the first clamping plate 46 and the second clamping plate 47 are mutually attached and separated from each other; The swing arm skeleton 34 also includes two hinge supports 32 symmetrically arranged on both sides of the outer wall of the swing arm skeleton 34. The surface of each hinge support 32 is rotatably connected to a swing arm strut 10. Each swing arm strut 10 is an arc-shaped rod. The top of each swing arm strut 10 is provided with a support opening sheet 48. The support opening sheet 48 is a thin wide iron sheet. An arc-shaped slide groove 30 is opened in the middle of the swing arm strut 10. The surface of the swing arm skeleton 34 is rotatably connected to a swing arm cylinder 31. The swing arm cylinder 31 has a piston rod extending upward, and the top of the piston rod of the swing arm cylinder 31 is slidably connected in the arc-shaped slide groove 30.

[0033] Specifically, in the present embodiment, the aluminum foil bag is horizontally transported to the bottom of the bag lifting cylinder 35. The present invention pushes the bag lifting bracket 36 to descend by the bag lifting cylinder 35. The bag lifting bracket 36 uses two bag lifting suction cups 37 to respectively absorb the two ends of the aluminum foil bag. The top surface of the aluminum foil bag with the bag opening facing forward is gently lifted horizontally upward, and the bottom surface of the aluminum foil bag naturally droops on the surface of the bag feeding bracket 38 by gravity, so that the aluminum foil bag opening and the aluminum foil bag belly are stretched open, and the swing arm frame 34 is swung downward to a vertical state, and the opening thin sheet 48 at the top of the swing arm support rod 10 on the side of the swing arm frame 34 is swung downward and extended into the aluminum foil bag opening, and the aluminum foil bag opening is stretched open by the width of the opening thin sheet 48. At this time, the bag lifting suction cup 37 is loosened, and the first clamping plate 46 and the second clamping plate 47 on both sides of the swing arm frame 34 are respectively lifted from The left and right sides of the aluminum foil bag opening are clamped symmetrically so that the two sides of the stretched aluminum foil bag opening are clamped, and the middle of the aluminum foil bag opening is stretched in a mouth shape by two stretching sheets 48, and is swung to a horizontal state by the swing arm skeleton 34 to transport the aluminum foil bag to the bottom of the powder injection tube 9. During the transportation process, since the swing arm skeleton 34 is swung to a horizontal state, the aluminum foil bag opening is stretched by the width of the stretching sheet 48 to maintain an open state, so that the aluminum foil bag opening is inserted in an inclined direction during the initial contact with the bottom of the powder injection tube 9, and when the swing arm skeleton 34 swings to a horizontal state, the aluminum foil bag opening is gradually inserted into the bottom of the powder injection tube 9 to a horizontal state. Such an operation can facilitate the aluminum foil bag opening to be inserted into the bottom of the powder injection tube 9 more smoothly, and the insertion process is not easy to get stuck on the bottom surface of the powder injection tube 9, and the automated insertion process is more solid and reliable.

[0034] The filling assembly includes a powder injection tube 9 arranged at the bottom of the top frame 12. The powder injection tube 9 is a hollow circular tube structure. A powder injection rod 14 is fixedly installed inside the powder injection tube 9. A joint is arranged at the top of the powder injection rod 14. The joint at the top of the powder injection rod 14 is connected with an external powder storage tank through a pipeline, and a quantitative material is pumped into the powder injection rod 14 by a quantitative pump inside the powder injection rod 14, and the material is injected downward through the powder injection rod 14. The bottom end of the powder injection rod 14 slightly extends toward the bottom of the powder injection tube 9, and emptying columns 15 are symmetrically arranged on the left and right sides of the powder injection rod 14. The emptying columns 15 on both sides can slide up and down and extend out on the surface of the powder injection rod 14, and the top ends of the emptying columns 15 on both sides are fixed on a lifting frame 13, and pneumatic cylinders 16 are symmetrically arranged on the left and right sides of the lifting frame 13.

[0035] Two bag mouth clamps 54 are symmetrically arranged on the front and rear side walls of the powder injection tube 9, each of which is an arc structure, and the outer wall of each bag mouth clamp 54 is provided with a clamp support rod 52, and the other end of the clamp support rod 52 is rotatably hinged to the surface of the powder injection tube 9, and two push rod cylinders 49 are symmetrically arranged on the front and rear side walls of the powder injection tube 9, each of which has a piston rod extending downward, and the top end of the piston rod of each push rod cylinder 49 is rotatably connected to the surface of the clamp support rod 52.

[0036] Double push cylinders 51 are symmetrically arranged on the left and right sides of the powder injection tube 9, wherein a spacing is arranged between the double push cylinder 51 on each side and the outer wall of the powder injection tube 9 for the support mouth sheet 48 to swing, a bottom fixed support rod 50 is fixedly installed on the top end of the double push cylinder 51 on each side, and the top end of the bottom fixed support rod 50 on each side is fixed to the top end of the top frame 12, and piston rods are symmetrically extended from the front and rear sides of the double push cylinder 51 on each side, and a bag mouth side clamp 53 is respectively arranged on the top end of the piston rod on the front and rear sides of the double push cylinder 51 on each side.

[0037] Specifically, in the present embodiment, the opening of the aluminum foil bag inserted into the bottom of the powder injection tube 9 is symmetrically clamped from the front and rear sides of the outer wall of the powder injection tube 9 by the bag opening clamping plate 54 of the outer wall of the powder injection tube 9. At this time, the swing arm support rod 10 is swung upward along the hinge support 32 of the swing arm frame 34 and is pulled out upward from the gap between the adjacent double-push cylinder 51 and the powder injection tube 9. During the upward pulling process, the swing arm support rod 10 will swing upward at a certain angle so that the support thin sheet 48 will not collide with the aluminum foil bag inserted into the bottom of the powder injection tube 9 when the swing arm frame 34 is retracted. The clamping plate 47 is released from the left and right sides of the aluminum foil bag opening, and the swing arm frame 34 retreats and swings to a vertical state, ready to pick up the next aluminum foil bag. When the bag opening clamping plate 54 clamps the aluminum foil bag on the outer wall of the powder injection tube 9, the left and right sides of the aluminum foil bag are clamped and sealed by the two bag opening side clamps 53 through the double push cylinders 51 on both sides of the powder injection tube 9. Since each bag opening side clamp 53 is horizontally arranged, horizontal clamping is performed from the left and right sides of the aluminum foil bag opening, thereby increasing the sealing of the aluminum foil bag opening during the powder filling process and reducing the leakage of powder from the aluminum foil bag opening.

[0038] Specifically, in the present embodiment, during the process of the powder injection tube 9 injecting powder into the aluminum foil bag, the present invention first extends downward from the bottom of the powder injection tube 9 through the emptying column 15, and the powder injection tube 9 will extend into the middle of the aluminum foil bag to isolate and support the inner wall of the aluminum foil bag adsorbed together, which is beneficial to reducing the impact on the inner wall of the aluminum foil bag caused by the powder injection rod 14 inside the powder injection tube 9 during the process of spraying powder downward. By reducing the impact of the initial injection of powder into the aluminum foil bag, it is beneficial to reduce the phenomenon of powder floating in the aluminum foil bag. At the same time, it can further prevent the aluminum foil bag from swinging or shaking during the initial injection of powder, which is beneficial to smoother initial injection of powder into the aluminum foil bag. The emptying column 15 is arranged inside the powder injection tube 9. During the powder injection process, as the amount of powder injected into the aluminum foil bag gradually increases, the emptying column 15 gradually rises and is withdrawn from the powder injection tube 9. The emptying column 15 is arranged inside the powder injection tube 9, which is beneficial to reducing the friction between the emptying column 15 and the aluminum foil bag during the process, and can increase the tightness of the aluminum foil bag opening during the powder injection process, effectively reducing the loss of powder from the aluminum foil bag opening.

[0039] Specifically, in this embodiment, the powder injection tube 9 of the present invention can inject a maximum of 25 kg of powder at one time during the powder filling process. When the aluminum foil bag is inserted into the bottom of the powder injection tube 9, the electric push rod 5 pushes the tray 7 to rise to the bottom of the aluminum foil bag. The bottom of the aluminum foil bag is supported by the tray 7, which can effectively reduce the force on the aluminum foil bag mouth during the filling process, and effectively improve the safety of the aluminum foil bag during the filling process.

[0040] Specifically, in this embodiment, after the powder filling inside the aluminum foil bag is completed, the sliding frame 25 moves to the bottom of the filling assembly and lands on the top of the sliding frame 25 through the clamping cylinder 3 29. At this time, the position of the clamping cylinder 3 29 at the top of the sliding frame 25 is just above the aluminum foil bag. The clamping cylinders 3 29 on both sides push the aluminum foil bag back and forth three times from the upper position of the aluminum foil bag. The tension of the aluminum foil bag mouth hanging on the powder injection tube 9 makes the upper half of the aluminum foil bag, which is hollow due to the lack of powder filling inside, tightened. The clamping cylinders 3 29 on both sides push the aluminum foil bag back and forth three times from the upper position of the aluminum foil bag to shake the powder inside the aluminum foil bag and reduce the phenomenon of powder hanging on the inner wall of the aluminum foil bag. This effectively facilitates the vacuum compression in the later stage.

[0041] Specifically, in the present embodiment, after the clamping cylinder three 29 is pushed back and forth three times, it is pushed upward by the lifting cylinder 27, and the clamping cylinder three 29 pushes the clamping plate 20 to clamp the top of the aluminum foil bag opening. At this time, the bag opening side clamping piece 53 and the bag opening clamping plate 54 at the top of the aluminum foil bag opening are loosened, and the clamping cylinder three 29 clamps the aluminum foil bag opening and drops downward synchronously with the tray 7. After dropping to the bottom, the clamping cylinder one 26 and the clamping cylinder two 28 are used to clamp the bottom abdomen of the aluminum foil bag and the middle of the aluminum foil bag respectively to improve the clamping firmness of the aluminum foil bag. The top surface of the tray 7 is a smooth plane, and the sliding frame 25 slides back and forth in the slide groove 23 to move the aluminum foil bag filled with powder to the bottom of the vacuum assembly for vacuuming.

[0042] The bag sealing assembly includes a suspended plastic sealing box 21 arranged at the bottom of the top frame 12, the outer wall of the suspended plastic sealing box 21 is provided with a supporting frame 22, and the supporting frame 22 is fixed to the bottom of the top frame 12. A sealing groove 2110 for the aluminum foil bag mouth to pass through is provided in the middle of the inner wall of the bottom surface of the suspended plastic sealing box 21, and the inner wall of the sealing groove 2110 is symmetrically provided with circulating and rotating bag pressing strips 2104 on both sides of the front and rear sides of the inner wall, and two bag pressing strips 2104 are provided on each side of the inner wall of the sealing groove 2110. 04 rotate parallel to each other, the surface of each bag pressing strip 2104 is flat, a hot melt wheel 2102 is arranged in the middle position of the two bag pressing strips 2104 on one side of the sealing groove 2110, and a bag pressing wheel 2101 is arranged in the middle position of the two bag pressing strips 2104 on the other side of the sealing groove 2110, an arc-shaped concave hot melt groove 2105 is provided in the middle position of the outer circumference of the hot melt wheel 2102, and an extrusion ring 2111 matched with the hot melt groove 2105 is arranged on the outer circumference of the bag pressing strip rotating wheel 2103.

[0043] Specifically, in the present embodiment, the aluminum foil bag moved to the bottom of the vacuum pumping assembly is extended into the aluminum foil bag by the vacuum suction rod 18, and the clamping plate 56 is pushed by the clamping cylinder 55 to clamp the opening of the aluminum foil bag firmly. The bottom of the vacuum suction rod 18 adopts a small suction force to absorb the air in the aluminum foil bag. The small suction force is not easy to absorb the powder in the aluminum foil bag. During the suction process, the vacuum suction rod 18 gradually rises upward, and at the same time cooperates with the clamping cylinder 1 26, the clamping cylinder 28, and the clamping cylinder 3 29 to clamp from bottom to top in sections, so that the air in the aluminum foil bag is discharged upward, so that the aluminum foil bag is vacuum adsorbed and clamped and sealed by the three clamping cylinders. At the same time, the vacuumized aluminum foil bag is transferred to the bag sealing assembly through the sliding frame 25.

[0044] Specifically, in the present embodiment, the opening of the aluminum foil bag transferred into the packaging component enters into the sealing groove 2110, and the two layers of the aluminum foil bag film entering into the sealing groove 2110 are pressed tightly by the two bag pressing strips 2104 on both sides of the sealing groove 2110 and dragged to the right. During the conveying process, the bag pressing strips 2104 and the conveyor belt 2 maintain a synchronous rotation speed, and the positions where the bag pressing strips 2104 on both sides of the sealing groove 2110 contact with the opening of the aluminum foil bag always remain in place and press tightly without relative sliding. No wrinkles are generated during the pressing and conveying process of the aluminum foil bag, and two bag pressing strips 2104 are provided on each side of the inner wall of the sealing groove 2110, so that during the hot melt plastic sealing process, the opening of the aluminum foil bag is pressed tightly by the upper and lower bag pressing strips 2104. The upper and lower positions of the hot-melt area of ​​the aluminum foil bag mouth are pressed and fixed. During the hot-melt process of the aluminum foil bag mouth, the periphery of the hot-melt area of ​​the aluminum foil bag is not easily deformed by hot-melt during the hot-melt shaping process. At the same time, the two layers of aluminum foil bag film at the aluminum foil bag mouth pass through between the bag pressing wheel 2101 and the hot-melt wheel 2102, and are rolled and pressed between the bag pressing wheel 2101 and the hot-melt wheel 2102, and cooperate with the arc-shaped hot-melt groove 2105 of the hot-melt wheel 2102, so that the hot-melt line of the aluminum foil bag mouth is wider, and the hot-melt seal is more firm and reliable. After the rolling hot-melt is completed, the aluminum foil bag is sealed and fastened, and then transported to the end of the conveyor belt 2 for stacking or transfer, and at this time, the sliding frame 25 continues to return to the filling assembly to repeat the above shaking bag three times for another aluminum foil bag. The sealed packaging of the aluminum foil bag is completed in a cycle.

[0045] Hot melt wheel shafts 2107 are symmetrically arranged at both ends of the hot melt wheel 2102, shaft sleeves 2106 are symmetrically arranged at both ends of the inner ring of the suspended plastic packaging chassis 21, two electrode terminals 2109 of positive and negative poles are arranged at one of the top ends of the hot melt wheel shaft 2107, one of the electrode terminals 2109 is a circular ring structure, and the other electrode terminal 2109 is a circle center structure, and the inner wall of the shaft sleeve 2106 is provided with conductive terminals 2108 that match the two electrode terminals 2109, and the conductive terminals 2108 are connected with the electrode terminals 2109 through the matching connection, so that the hot melt wheel 2102 can maintain normal and stable power supply during the rotation process, so that the inner wall of the hot melt groove 2105 with an arc structure on the inner wall of the hot melt wheel 2102 can maintain uniform heating, and the heating principle of the hot melt groove 2105 adopts the resistance heating principle, which is the existing technology.

[0046] The vacuum assembly includes a lifting frame 17 disposed at the top of the top frame 12, and two vacuum suction rods 18 are disposed at the bottom of the lifting frame 17. A joint is disposed at the top of each vacuum suction rod 18, and the joint at the top of the vacuum suction rod 18 is connected to an external vacuum pump through a pipeline. The vacuum suction rod 18 extends into the opening of the aluminum foil bag filled with powder, and the powder in the aluminum foil bag is pumped by the vacuum pump with a small suction force, thereby achieving pumping. A pneumatic cylinder 19 is disposed on the left and right sides of the lifting frame 17; Two clamping cylinders 55 are provided at the bottom end of the top frame 12. The two clamping cylinders 55 have piston rods extending toward the middle direction. Clamping plates 56 are provided at the top ends of the piston rods of the two clamping cylinders 55. Each clamping plate 56 fits the outer wall contour of the vacuum suction rod 18, and a clearance groove matching the vacuum suction rod 18 is provided on the surface of the clamping plate 56.

[0047] Specifically, in the present embodiment, the aluminum foil bag moved to the bottom of the vacuum pumping assembly is extended into the aluminum foil bag by the vacuum suction rod 18, and the clamping plate 56 is pushed by the clamping cylinder 55 to clamp the opening of the aluminum foil bag firmly. The bottom of the vacuum suction rod 18 adopts a small suction force to absorb the air in the aluminum foil bag. The small suction force is not easy to absorb the powder in the aluminum foil bag. During the suction process, the vacuum suction rod 18 gradually rises upward, and at the same time cooperates with the clamping cylinder 1 26, the clamping cylinder 28, and the clamping cylinder 3 29 to clamp from bottom to top in sections, so that the air in the aluminum foil bag is discharged upward, so that the aluminum foil bag is vacuum adsorbed and clamped and sealed by the three clamping cylinders. At the same time, the vacuumized aluminum foil bag is transferred to the bag sealing assembly through the sliding frame 25.

[0048] In addition, the aluminum foil bag described in this embodiment is an aluminum foil plastic bag, which can be hot-melt sealed. The aluminum foil plastic bag is usually made of aluminum foil and other plastic materials (such as polyethylene or polypropylene). Since the aluminum foil itself does not have heat-sealing properties, when hot-melt sealing is performed, the plastic material compounded with it is mainly used for heat sealing. In the prior art, other film bag materials that can be hot-melt sealed can also be used as a substitute for the aluminum foil bag in this embodiment.

[0049] The basic principles, main features and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and the description in the specification are only to illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A method for producing a drug having a gene recombination functional enzyme, characterized in that: The method for manufacturing a drug having a gene recombination functional enzyme mainly comprises the following steps: S1. Plant pretreatment: sorting, cleaning and drying the plants to obtain the required super-enriched plants; S2, preparing ultrafiltration concentrate: immersing the pretreated super-enriched plant in a buffer solution containing a metal chelating agent, then crushing and homogenizing, filtering the obtained homogenate, repeating the leaching and filtration at least once, and combining the filtrate; adding ammonium sulfate to the super-enriched plant treatment solution to be salted out, standing and centrifuging to obtain the supernatant; adding deionized water to the super-enriched plant treatment solution to be removed for dilution, and ultrafiltration until the filtrate is clear and colorless, and taking the retentate; ultrafiltration and concentration of the super-enriched plant treatment solution to be concentrated; S3, precipitation and drying: slowly adding acetone to the super-enriched plant treatment solution to be precipitated and enriched until the volume concentration of acetone in the solution reaches 60-70%, standing, then centrifuging to obtain a precipitate, removing acetone, and freeze-drying the superoxide dismutase-enriched precipitate obtained in the precipitation and enrichment step to prepare a superoxide dismutase-enriched dry powder; S4, powder transfer and bulk bag making: the prepared superoxide dismutase enriched dry powder is transferred to a powder storage tank for sealed filling, and the powder is quantitatively transported to a drug filling device through the powder storage tank; S5. Automatic picking of aluminum foil bags: set the automatic picking of aluminum foil bags to face the opening of the aluminum foil bags forward and place the aluminum foil bags flat on the bag feeding bracket, fix the aluminum foil bags with the bag opening bracket and open the opening of the aluminum foil bags; S6. Insert the opening of the aluminum foil bag into the bottom of the powder injection tube: a swing arm frame that swings back and forth vertically is set to pick up the opened opening of the aluminum foil bag and swing it vertically, so that the aluminum foil bag originally placed flat is swung to a vertical state, and the opening of the aluminum foil bag is always kept in an open state during the swinging process, and the opened opening of the aluminum foil bag is inserted into the outer wall of the powder injection tube at an inclined angle; S7, clamping the aluminum foil bag opening: a clamping device is set on the aluminum foil bag opening on the outer wall of the powder injection tube to tightly wrap the aluminum foil bag opening on the outer wall of the powder injection tube; S8, emptying rod leading, powder injection: In step S4, the powder storage tank quantitatively transports the powder to the powder injection tube, and the powder is sprayed into the aluminum foil bag wrapped on the outer wall of the powder injection tube through the powder injection tube. Before spraying the powder, the emptying rod is arranged to extend into the aluminum foil bag to open the aluminum foil bag with the inner wall attached together, and the powder injection tube injects a quantitative amount of powder into the aluminum foil bag; S9, vacuum compression: vacuum compression is performed on the inside of the aluminum foil bag filled with quantitative powder, and a vacuum suction rod is set to extend into the aluminum foil bag to extract the air in the aluminum foil bag. The vacuum suction rod slowly extends upwards during the process of extracting air from the aluminum foil bag, and cooperates with the clamping plate to clamp upwards in sections until the air in the aluminum foil bag is exhausted; S10, aluminum foil bag plastic sealing: the aluminum foil bag opening from which air has been exhausted is horizontally slid and hot-melt plastic sealed, and the aluminum foil bag opening is hot-melt plastic sealed and fastened by means of bag pressing wheel and hot-melt wheel rolling and hot-melt during the horizontal sliding process of the aluminum foil bag; S11. Bulk bag transfer: The hot-melt plastic-sealed aluminum foil bag is transferred to the next station by a robot for transportation or long-term storage; S12, mixing: putting the large bag of powder prepared in step S11 into a mixing device, adding drug components and mixing the powder until the mixture is uniform to obtain drug powder; S13, bagging and packaging: quantitatively divide the drug powder obtained in step S12 into small bags and seal them to obtain finished drugs.

2. The method for producing a drug having a gene recombination functional enzyme according to claim 1, characterized in that: The steps S4-S10 described above are mainly completed by a drug filling device, which includes a bottom chassis arranged on the bottom surface, and a top frame arranged in the middle of the house, wherein the bottom of the top frame in the middle of the house is provided with an automatic bag supply assembly, a filling assembly, a vacuum assembly, and a bag sealing assembly; the filling assembly, the vacuum assembly, and the bag sealing assembly are arranged in a row at the bottom of the top frame, The bottom chassis are provided with two at the bottom of the top frame, and an intermittently rotating conveyor belt is provided in the middle of the two bottom chassis. A push rod frame 1 is provided in the middle of the two bottom chassis at the bottom of the filling component, and a reciprocating lifting tray is provided at the top of the push rod frame 1. A slide groove is provided at the top of each of the two bottom chassis, and a sliding frame is slidably connected to the inside of each slide groove, and a transmission screw is rotatably connected to the inside of the slide groove. The sliding frame slidably connected to the transmission screw in the slide groove and the transmission screw Screw transmission, a clamping cylinder 1 is provided in the middle of the sliding frame on each side, and a clamping plate is extended toward the middle direction of the bottom chassis from the clamping cylinder 1 on both sides, and a clamping cylinder 2 is provided at the top of the sliding frame on each side, and a lifting cylinder is fixedly installed on the top of the sliding frame on each side, and a push rod is extended upward from the lifting cylinder, and a clamping cylinder 3 is fixedly installed on the top of the push rod of the lifting cylinder, and a clamping plate is also extended toward the middle direction of the bottom chassis from the clamping cylinder 3 and the clamping cylinder 2.

3. The method for producing a drug having a gene recombination functional enzyme according to claim 1, characterized in that: The automatic bag supply assembly is arranged next to the filling assembly, and the automatic bag supply assembly includes a bag lifting cylinder fixedly installed at the rear end of the top frame, a piston rod extending from the bottom of the bag lifting cylinder, a bag grabbing suction cup is arranged at the bottom of the piston rod of the bag lifting cylinder, and two intermittently circulating chains are arranged below the bag lifting cylinder, a bag feeding bracket is arranged at an equal interval between the two circulating chains, and a distance is set between two adjacent sections of the bag feeding bracket, and an aluminum foil bag stacking box is arranged between the two circulating chains, and a plurality of aluminum foil bags with the bag openings facing the same direction and neatly stacked are placed on the top of the aluminum foil bag stacking box.

4. The method for producing a drug having a gene recombination functional enzyme according to claim 3, characterized in that: The bag lifting cylinder is located on one side of the intermittently rotating chain, and a bag lifting cylinder is arranged above the other side of the intermittently rotating chain. A piston rod extends downward from the bottom of the bag lifting cylinder, and a horizontally placed bag lifting bracket is fixed to the bottom end of the piston rod of the bag lifting cylinder. The bag lifting bracket is arranged in the middle of the two intermittently rotating chains, and the bag lifting bracket is parallel to the two intermittently rotating chains. A bag lifting suction cup is arranged at the bottom of the bag lifting bracket.

5. The method for producing a drug having a gene recombination functional enzyme according to claim 3, characterized in that: The middle part of the top frame is rotatably connected with a rotating shaft, and a swing arm frame is fixedly installed on the outer wall of the rotating shaft. The swing arm frame includes two rack slide grooves arranged on the outer wall of the swing arm frame, and the inner walls of the two rack slide grooves are slidably connected with second racks, and the middle position of the two second racks is rotatably connected with a synchronous gear, and a first clamping plate is arranged at the end of one of the second racks, and a second clamping plate is arranged at the end of the other second rack, and the first clamping plate and the second clamping plate are attached to each other and separated from each other; The swing arm frame also includes two hinge supports symmetrically arranged on both sides of the outer wall of the swing arm frame, each of the hinge supports is rotatably connected to a swing arm strut on the surface, each of the swing arm struts is an arc-shaped rod, and a support opening sheet is arranged on the top of each swing arm strut, and the support opening sheet is a thin wide iron sheet, and an arc-shaped slide groove is opened in the middle of the swing arm strut, and a swing arm cylinder is rotatably connected to the surface of the swing arm frame, and a piston rod is extended upward from the swing arm cylinder, and the top of the piston rod of the swing arm cylinder is slidably connected in the arc-shaped slide groove.

6. The method for producing a drug having a gene recombination functional enzyme according to claim 1, characterized in that: The filling assembly includes a powder injection tube arranged at the bottom of the top frame, the powder injection tube is a circular tube structure with a hollow interior, a powder injection rod is fixedly installed inside the powder injection tube, the bottom end of the powder injection rod slightly extends toward the bottom of the powder injection tube, and emptying columns are symmetrically arranged on the left and right sides of the powder injection rod, and the emptying columns on both sides can slide up and down on the surface of the powder injection rod and extend out, and the top ends of the emptying columns on both sides are fixed on the lifting frame 1, and the left and right sides of the lifting frame 1 are symmetrically arranged with a pneumatic cylinder 1; Two bag mouth clamps are symmetrically arranged on the front and rear side walls of the powder injection tube, each of the bag mouth clamps is an arc structure, and the outer wall of each bag mouth clamp is provided with a clamp support rod, and the other end of the clamp support rod is rotatably hinged to the surface of the powder injection tube. Two push rod cylinders are symmetrically arranged on the front and rear side walls of the powder injection tube, and the top end of the piston rod of each push rod cylinder is rotatably connected to the surface of the clamp support rod.

7. The method for producing a drug having a gene recombination functional enzyme according to claim 6, characterized in that: Double push cylinders are symmetrically arranged on the left and right sides of the powder injection tube, wherein a spacing is arranged between the double push cylinder on each side and the outer wall of the powder injection tube, a bottom fixed support rod is fixedly installed on the top of the double push cylinder on each side, and the top of the bottom fixed support rod on each side is fixed to the top of the top frame, piston rods are symmetrically extended from the front and rear sides of the double push cylinder on each side, and a bag mouth side clamp is arranged on the top of the piston rod on the front and rear sides of the double push cylinder on each side.

8. The method for producing a drug having a gene recombination functional enzyme according to claim 2, characterized in that: The bag sealing assembly includes a suspended plastic sealing box arranged at the bottom of the top frame, the outer wall of the suspended plastic sealing box is provided with a supporting frame, and the supporting frame is fixed to the bottom of the top frame, and a sealing groove for the aluminum foil bag to pass through is provided in the middle of the inner wall of the bottom surface of the suspended plastic sealing box, and the inner wall of the sealing groove is symmetrically provided with circulating bag pressing strips on the front and back sides, and the bag pressing strips are provided with two on each side of the inner wall of the sealing groove, and the two bag pressing strips rotate in parallel, and the surface of each bag pressing strip is flat, and a hot melt wheel is provided in the middle position of the two bag pressing strips on one side of the sealing groove, and a bag pressing wheel is provided in the middle position of the two bag pressing strips on the other side of the sealing groove, and an arc-shaped concave hot melt groove is provided in the middle position of the outer cylindrical surface of the hot melt wheel, and an extrusion ring matching the hot melt groove is provided on the outer cylindrical surface of the bag pressing strip rotating wheel.

9. The method for producing a drug having a gene recombination functional enzyme according to claim 8, characterized in that: Hot melt wheel shafts are symmetrically arranged at both ends of the hot melt wheel, shaft sleeves are symmetrically arranged at both ends of the inner ring of the suspended plastic packaging chassis, two electrode terminals of positive and negative poles are arranged at one of the top ends of the hot melt wheel shaft, one of the electrode terminals is a circular ring structure, and the other electrode terminal is a circle center structure, and the inner wall of the shaft sleeve is provided with conductive terminals matching the two electrode terminals.

10. The method for producing a drug having a gene recombination functional enzyme according to claim 2, characterized in that: The vacuum assembly includes a lifting frame 2 arranged at the top of the top frame, two vacuum suction rods are arranged at the bottom end of the lifting frame 2, and a pneumatic cylinder 2 is arranged on the left and right sides of the lifting frame 2 respectively; Two clamping cylinders are arranged at the bottom end of the top frame, and piston rods are extended toward the middle of the two clamping cylinders. Clamping plates are arranged at the top ends of the piston rods of the two clamping cylinders, and each clamping plate fits the outer wall contour of the vacuum suction rod.

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