Miniature hydrocyclone for high-flux blood separation

Abstract

The invention relates to the technical field of hydrocyclones, and provides a miniature hydrocyclone for high-flux blood separation, the miniature hydrocyclone comprises a cyclone separation mechanism and a blood conveying mechanism, the cyclone separation mechanism is fixedly connected to a mounting base, and the cyclone separation mechanism is used for driving blood to flow and performing centrifugal separation on the blood; the blood conveying mechanism is fixedly connected to the mounting base, the blood conveying mechanism is communicated with the cyclone separation equipment, the plunger pump is communicated with the blood conveying mechanism, the blood conveying mechanism is used for conveying blood into the cyclone separation mechanism through the plunger pump, the internal diameter of the cyclone separator is 4 mm, the cone angle of 6 degrees is arranged on the upper cone part of the cyclone separator, and the lower cone part of the cyclone separator is communicated with the plunger pump. By means of the technical scheme, the problem that blood separation equipment in the prior art is difficult to achieve efficient blood separation is solved.

Description

Technical Field

[0001] The invention relates to the technical field of hydrocyclones, and in particular to a micro hydrocyclone for high-throughput blood separation. Background Art

[0002] Currently, efficient blood separation is crucial in the clinical and biopharmaceutical fields. It ensures the rapid separation and timely storage of blood components to maintain their functional integrity. It is suitable for component transfusion, blood product therapy and emergency treatment. Among them, platelet-rich plasma (PRP) collection is one of the key applications, which requires the rapid separation of plasma with platelet concentrations higher than the baseline value. PRP therapy can directly deliver high concentrations of platelets to damaged tissues, which are rich in growth factors and cytokines. It has been widely used to promote tissue regeneration and accelerate wound healing and anti-inflammatory treatment. However, existing blood separation technologies face significant challenges. Current blood separation methods include magnetic activated cell sorting (MACS), flow cytometry (FACS), and size filtration-based methods. Although these blood separation methods can provide higher separation accuracy, they usually require additional pretreatment steps, such as labeling cells or adding chemical reagents. These steps increase the complexity and cost of the operation. At the same time, the throughput limitations of the equipment make it difficult to meet large-scale clinical or industrial needs. Microfluidic devices have attracted much attention because of their ability to precisely control cell flow, but their flow limitations (usually in the range of microliters to milliliters per minute) and easy clogging make them inefficient when processing high-viscosity whole blood samples, so it is difficult to meet the use of a wide range of applications.

[0003] The method widely used at present is the density-based centrifugal separation method, which separates blood components into layers through the centrifugal force generated by rotation (the upper layer is mainly plasma ~45%, the middle layer is mainly white blood cells and platelets ~1%, and the lower layer is mainly red blood cells ~54%, with no obvious boundaries between the three layers). However, this method has obvious defects: First, due to the similar density of blood cell components, most platelets gather in the middle layer after centrifugation, and the extracted plasma has a small content of platelets and contains some red blood cells, so the current centrifugal method cannot achieve blood cell size classification. In addition, the centrifugal separation process takes a long time, and it usually takes several hours to complete multiple steps of manual operation; secondly, centrifugal separation has high requirements for technical personnel, and the results are easily affected by human factors, resulting in inconsistent separation effects, which will seriously affect the quality and therapeutic effects of products such as PRP.

[0004] Hydrocyclones have become a viable solution due to their high-speed separation capabilities. The technology is based on the principle of centrifugal separation and uses the density difference between different phases for separation. Unlike centrifuges that generate artificial gravity by rotating the chamber at high speed, hydrocyclones convert the forced jet flow into a high-speed vortex flow to form a centrifugal force that is mainly dominated by the tangential velocity. By adjusting the inlet flow rate and the characteristic diameter of the hydrocyclone, the artificial gravity generated can reach thousands of g instantly, thus eliminating the long acceleration process required for centrifuges. In addition, under turbulent conditions, the dispersed phase will undergo vortex surges, that is, particles will preferentially migrate from high turbulence areas to low turbulence areas, increasing the relative velocity difference between the phases and thus improving the separation efficiency. Hydrocyclones also have fixed structural components, which not only provides higher structural durability, but also reduces manufacturing and maintenance costs. These advantages make it particularly suitable for long-term continuous operation and have been widely used in environmental engineering, mineral engineering, food engineering, and oil and gas engineering.

[0005] To adapt hydrocyclone technology to biological samples, we invented a micro hydrocyclone that maintains cell viability through a special design for continuous high-speed separation of blood.

[0006] We take advantage of the shear-thinning property of blood, which significantly reduces the dynamic viscosity of blood at high inlet flow rates, thereby enhancing the radial migration of cells. This property enables the hemocyclone to achieve efficient blood cell fractionation at lower dilution factors without the need for additional measures to reduce the static viscosity of complex blood samples. This unique capability makes the hemocyclone a superior tool for low-dilution blood sample processing, providing high-quality blood separation products suitable for downstream medical and biological applications.

[0007] In this patent, we invented a blood cyclone, which is a 2-8mm micro hydrocyclone system, as an innovative solution for efficient blood separation for rapid blood separation, suitable for processing complex, high viscosity, high cell density non-Newtonian biofluids.

[0008] The system is suitable for efficient separation of large volume blood products. Under high inlet flow rate and high concentration conditions, the system can accurately and efficiently separate blood components while maintaining the biological activity of cell products. It meets the needs of the biopharmaceutical industry, such as platelet-rich plasma collection.

[0009] The blood cyclone is also an economical and efficient rapid separation solution designed for point-of-care applications, allowing the separated blood components to be used immediately, avoiding the functional loss that may occur during traditional time-consuming and labor-intensive processing and transportation.

[0010] Therefore, the clinical and biopharmaceutical industries are in urgent need of a method that can efficiently separate blood components and size fractionate blood cells to meet the needs of large-scale production and bedside applications. Summary of the invention

[0011] The present invention provides a micro hydrocyclone for high-throughput blood separation, which solves the problem that the blood separation equipment in the prior art is difficult to meet the requirements of efficient blood component separation and blood cell size classification.

[0012] The technical solution of the present invention is as follows: A micro hydrocyclone for high-throughput blood separation, comprising a mounting base, a motor and a plunger pump, wherein the output end of the motor is connected to the plunger pump, the motor and the plunger pump are fixedly mounted on the mounting base, and further comprising a cyclone separation mechanism and a blood conveying mechanism, wherein the cyclone separation mechanism is fixedly connected to the mounting base, the cyclone separation mechanism is used to drive blood flow and centrifuge the blood, the blood conveying mechanism is fixedly connected to the mounting base, the blood conveying mechanism is communicated with the cyclone separation device, the plunger pump is communicated with the blood conveying mechanism, the blood conveying mechanism is used to convey blood to the cyclone separation mechanism through the plunger pump, wherein the cyclone separation mechanism is provided with a conveying assembly and a cyclone assembly, the conveying assembly is fixedly connected to the mounting base, the conveying assembly is communicated with the blood separation mechanism, the conveying assembly is used to guide blood, the cyclone assembly is fixedly arranged on the conveying assembly, the cyclone assembly is communicated with the conveying assembly, and the cyclone assembly is used to centrifuge blood.

[0013] The blood delivery mechanism includes a storage beaker, a feed pipeline, a bypass beaker and a delivery pipeline. The storage beaker is fixedly connected to the mounting base. One end of the feed pipeline is connected to the storage beaker, and the other end is connected to the liquid inlet of the plunger pump. The bypass beaker is fixedly connected to the mounting base. The delivery pipeline is respectively connected to the bypass beaker, the liquid outlet of the plunger pump and the delivery assembly. A control valve is fixedly connected to the end of the delivery pipeline connected to the bypass beaker.

[0014] The conveying assembly includes a fixed frame, a feeding pipe, a plasma beaker and a red blood cell beaker. The fixed frame is fixedly connected to the mounting base, the feeding pipe is fixedly connected to the fixed frame, both ends of the feeding pipe are respectively connected to the swirl assembly and the conveying pipeline, the plasma beaker is arranged on the mounting base, and the red blood cell beaker is arranged on the mounting base.

[0015] The cyclone assembly includes a fixed shell, a Y-shaped feed pipe and a cyclone separator, the fixed shell is fixedly connected to the fixed frame, the Y-shaped feed pipe is fixedly connected in the fixed shell, the Y-shaped feed pipe is connected to the feeding pipe, the cyclone separator is fixedly connected in the fixed shell, and the cyclone separator is connected to the Y-shaped feed pipe.

[0016] The cyclone separator is provided with a plasma outlet, a red blood cell outlet and a spiral inlet. The plasma outlet is opened at the top of the cyclone separator and is connected to the plasma beaker. The red blood cell outlet is opened at the bottom of the cyclone separator and is connected to the red blood cell beaker. Two spiral inlets are provided, both of which are fixedly connected to the top of the cyclone separator, and the two spiral inlets are symmetrically arranged, and both of which are connected to a side of the Y-shaped feeding tube away from the loading tube.

[0017] The cyclone separator is configured such that the inner cylindrical chamber has a diameter (D) of 2-8 mm.

[0018] The cone angle of the conical chamber on the cyclone separator is 2-6°.

[0019] The length of the inlet spiral is between 1.5πD and 2πD.

[0020] The insertion angle between the spiral inlet and the cyclone separator is 5-20°.

[0021] The working principle and beneficial effects of the present invention are: 1. In the present invention, by setting a cyclone separator, the double inlet design ensures the symmetry of the velocity distribution of the inner and outer vortex flows, which is crucial for maintaining the activity of blood cells and their volume distribution gradient. The 12° insertion angle of the spiral inlet provides a smooth transition from the linear jet to the tangential vortex motion, reducing excessive turbulence near the inlet area. The cone portion of the cyclone separator is provided with a 6° cone angle. The 6° cone angle design prolongs the residence time of blood cells in the cyclone separator and provides sufficient momentum compensation for the rotating flow, thereby improving the overall separation efficiency. The cyclone separator is set to an internal diameter of 4 mm, and a combination of vortex surge and centrifugal force is used to achieve size-based cell component classification; 2. In the present invention, by setting a cyclone separation mechanism, compared with the existing centrifugal separation method, the cyclone separator of the present application shows significant advantages in processing speed and separation efficiency: the processing time is significantly shortened: the equipment can process 300mL of blood per minute, while the traditional centrifugal method usually takes several hours; the separation accuracy is higher: the equipment effectively realizes the classification of platelets and red blood cells, avoids red blood cell contamination, improves the quality and consistency of platelet-rich plasma (PRP), and achieves improved blood separation efficiency through a simpler operation method and a higher blood separation speed. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0023] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is another perspective structural schematic diagram of the whole of the present invention; Figure 3 It is a schematic diagram of the structure of the fixed frame and the conveying pipeline in the present invention; Figure 4 It is a schematic diagram of the internal cross-sectional structure of the fixed housing in the present invention; Figure 5 It is a schematic diagram of the structure of the cyclone separator and the Y-shaped feed pipe in the present invention; Figure 6 It is a schematic diagram of the internal cross-sectional structure of the cyclone separator in the present invention.

[0024] In the figure: 1. Mounting base; 2. Motor; 3. Plunger pump; 4. Storage beaker; 5. Feed pipeline; 6. Bypass beaker; 7. Delivery pipeline; 8. Control valve; 9. Fixed frame; 10. Feed pipe; 11. Plasma beaker; 12. Red blood cell beaker; 13. Fixed shell; 14. Y-type feed pipe; 15. Cyclone separator; 16. Plasma outlet; 17. Red blood cell outlet; 18. Spiral inlet. DETAILED DESCRIPTION

[0025] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Embodiment 1 like Figures 1 to 6As shown, this embodiment proposes a micro hydrocyclone for high-throughput blood separation, including a mounting base 1, a motor 2 and a plunger pump 3, wherein the output end of the motor 2 is connected to the plunger pump 3, and the motor 2 and the plunger pump 3 are fixedly mounted on the mounting base 1, and also include a cyclone separation mechanism and a blood conveying mechanism, wherein the cyclone separation mechanism is fixedly connected to the mounting base 1, and the cyclone separation mechanism is used to drive blood flow and centrifuge the blood, and the blood conveying mechanism is fixedly connected to the mounting base 1, and the blood conveying mechanism is connected to the cyclone separation device, and the plunger pump 3 is connected to the blood conveying mechanism, and the blood conveying mechanism is used to convey blood to the cyclone separation mechanism through the plunger pump 3, wherein a conveying component and a cyclone component are provided in the cyclone separation mechanism, and the conveying component is fixedly connected to the mounting base 1, and the conveying component is connected to the blood separation mechanism, and the conveying component is used to guide blood, and the cyclone component is fixedly arranged on the conveying component, and the cyclone component is connected to the conveying component, and the cyclone component is used to centrifuge the blood.

[0027] like Figures 1 to 3 As shown, the blood delivery mechanism includes a storage beaker 4, a feed pipeline 5, a bypass beaker 6 and a delivery pipeline 7. The storage beaker 4 is fixedly connected to the mounting base 1. One end of the feed pipeline 5 is connected to the storage beaker 4, and the other end is connected to the liquid inlet of the plunger pump 3. The bypass beaker 6 is fixedly connected to the mounting base 1. The delivery pipeline 7 is respectively connected to the bypass beaker 6, the liquid outlet of the plunger pump 3 and the delivery component. A control valve 8 is fixedly connected to one end of the delivery pipeline 7 connected to the bypass beaker 6. The blood in the storage beaker 4 is passed through the delivery pipeline 7 through the plunger pump 3. The blood is transported to the upper feeding tube 10. A pressure gauge can be set on the conveying pipeline 7 to detect the pressure to ensure that the pressure is within a safe range. During the process of blood being transported to the feeding tube 10 through the conveying pipeline 7, the blood pressure and flow rate entering the Y-type feeding tube 14 through the feeding tube 10 can be controlled by the control valve 8. By opening the control valve 8, the blood in the conveying pipeline can be transported to the bypass beaker 6. Through the control of the control valve 8, sampling can be performed after ensuring that the blood flow rate in the cyclone separator 15 is running stably, thereby ensuring the reliability and accuracy of the sample.

[0028] like Figures 1 to 3 As shown, the conveying assembly includes a fixed frame 9, a feeding tube 10, a plasma beaker 11 and a red blood cell beaker 12. The fixed frame 9 is fixedly connected to the mounting base 1, the feeding tube 10 is fixedly connected to the fixed frame 9, both ends of the feeding tube 10 are respectively connected to the swirl assembly and the conveying pipeline 7, the plasma beaker 11 is arranged on the mounting base 1, and the red blood cell beaker 12 is arranged on the mounting base 1. The stability of the continuous operation room is ensured by arranging a fixed frame 9 and a fixed shell 13 that can withstand a pressure of up to 80 Bar.

[0029] like Figures 4 to 6As shown, the cyclone assembly includes a fixed shell 13, a Y-shaped feed pipe 14 and a cyclone separator 15. The fixed shell 13 is fixedly connected to the fixed frame 9, the Y-shaped feed pipe 14 is fixedly connected in the fixed shell 13, the Y-shaped feed pipe 14 is connected to the feeding pipe 10, the cyclone separator 15 is fixedly connected in the fixed shell 13, the cyclone separator 15 is connected to the Y-shaped feed pipe 14, and the cyclone separator 15 can be manufactured by 3D printing, and then the wax support is melted in an oven, the residual wax is cleaned by ultrasound, and finally polished with a polishing liquid to improve the smoothness of the inner surface of the cyclone separator 15 to reduce plasma turbulence, energy loss and cell inactivation.

[0030] like Figures 4 to 6 As shown, the cyclone separator 15 is provided with a plasma outlet 16, a red blood cell outlet 17 and a spiral inlet 18. The plasma outlet 16 is opened at the top of the cyclone separator 15, and the plasma outlet 16 is connected to the plasma beaker 11. The red blood cell outlet 17 is opened at the bottom of the cyclone separator 15, and the red blood cell outlet 17 is connected to the red blood cell beaker 12. There are two spiral inlets 18, and the two spiral inlets 18 are fixedly connected to the top of the cyclone separator 15. The two spiral inlets 18 are symmetrically arranged, and the two spiral inlets 18 are connected to the side of the Y-shaped feeding tube 14 away from the feeding tube 10. The insertion angle of the spiral inlet 18 and the cyclone separator 15 is 5-20°, and the inlet The length of the spiral is between 1.5πD and 2πD. By setting the angle of the spiral inlet 18, the linear jet flow is ensured to smoothly transition to the tangential rotating flow, reducing the turbulence in the inlet area. The double inlet design ensures the symmetry of the velocity distribution of the inner and outer vortex flows, which is essential for maintaining the activity of blood cells and their volume distribution gradient. The 12° insertion angle of the spiral inlet 18 provides a smooth transition from the linear jet to the tangential vortex motion, reducing excessive turbulence near the inlet area. The cyclone in the present invention needs to be made with high precision, which can be completed by 3D printing, CNC or injection molding respectively. The inner surface is treated with a micro-particle polishing liquid to make the chamber wall transparent and control the roughness.

[0031] In the existing patents, the separation effect of blood is enhanced by generating sufficient centrifugal force. At the same time, the cyclone separator 15 is set with a water level culture medium, so its shear rate is used for separation of water, and the viscosity of blood is three to four times that of water. As a non-Newtonian fluid, its viscosity decreases significantly with the increase of shear rate. Therefore, the cyclone separator 15 in some existing patents is difficult to use for separation of whole blood. The size and structure of the cyclone separator 15 in the present application are designed for blood, and the shear thinning characteristics of blood are utilized. As the flow rate increases, the viscosity of blood decreases. The cyclone separator 15 in the present application is designed to not only generate sufficient centrifugal force, but also trigger the shear thinning characteristics of blood and ensure the formation of turbo electrophoresis, so that blood cells and proteins in the blood can be effectively separated.

[0032] The cyclone separator 15 is set to an internal cylindrical chamber diameter (D) of 2-8mm, and uses a combination of turbo electrophoresis and centrifugal force to achieve size-based cell component classification. It can handle the classification of blood cells such as red blood cells (~7.7 μm), white blood cells (~15 μm) or platelets (~2 μm), which is the core of the cyclone separator 15 design. The diameter of the cyclone separator 15 (less than 4 mm) is the key to produce turbo electrophoresis necessary for handling high viscosity blood as well as intercellular classification and cell protein classification.

[0033] The cone portion of the cyclone separator 15 is provided with a cone angle of 2-6°. The 6° cone angle design prolongs the residence time of blood cells in the cyclone separator 15 and provides sufficient momentum compensation for the rotating flow, thereby improving the overall separation efficiency.

[0034] During actual operation, the optimal inlet velocity range of blood is 8-10m / s. Under this condition, the device achieves 85.86% red blood cell recovery efficiency and 78% platelet activity, and can process more than 300mL of blood samples per minute, which is 10 times the efficiency of traditional centrifugal equipment. It supports whole blood samples with a dilution ratio of 1:5-1:20, reduces sample pretreatment steps, and improves the efficiency of blood separation processing.

[0035] This blood cyclone has a wide range of applications, not limited to the separation of PRP. It can be used for high-throughput separation of cells, proteins and antibodies in blood, body fluids or culture media, as well as small molecule components (cell protectants, toxic metabolites), to obtain pure blood cell products. In addition, by simultaneously introducing microbubbles or charged microbubbles, the separation efficiency can be further improved and the separation effect can be optimized. At the same time, the device can be used for the treatment of sickle cell anemia. Based on the principle of cell size and shape classification by the cyclone, sickle red blood cells can be separated from whole blood and then reinfused to improve the patient's blood composition.

[0036] In this embodiment, the plunger pump 3 transports the blood in the storage beaker 4 to the delivery pipeline 7, the blood enters the spiral inlet 18 through the Y-shaped feeding tube 14, and the flow rate and pressure of the blood entering the feeding tube 10 are adjusted by controlling the control valve 8. The blood undergoes centrifugal motion in the cyclone separator 15, and plasma and platelets are transported to the plasma beaker 11 through the plasma outlet 16, and red blood cells and proteins are transported to the red blood cell beaker 12 through the red blood cell outlet 17.

[0037] In the present invention, the control system of the hemocyclone uses a bypass valve design. The assembly of the entire cup is as follows: the mini hydrocyclone component is fixed to the metal feed pipe by an aluminum clamp, which can withstand a pressure of up to 80Bar in extreme pressure tests.

[0038] To ensure stable and continuous operation, a plunger pump is used to provide a high-pressure feed flow. The blood sample is first sucked in and pressurized by the plunger pump and then sent to the mini hydrocyclone. A pressure gauge is installed in the system to monitor the operating status and ensure that the pressure is within a safe range. At the same time, the flow and pressure entering the mini hydrocyclone are controlled by a bypass valve. After each adjustment of the inlet speed, the steady-state operation of the mini hydrocyclone is confirmed before sampling to ensure the reliability of the collected samples and the accuracy of the performance indicators. The overflow and underflow are guided into the test tube through a PTFE tube for subsequent analysis. The optically transparent properties of the cyclone material allow limited real-time observation of the separation process.

[0039] Blood enters the device at high pressure through a plunger pump, forming internal and external vortex flows. High-density red blood cells are pushed to the outer wall of the chamber under the action of centrifugal force and discharged from the bottom outlet; low-density plasma and platelets are discharged from the top outlet through the internal vortex flow. The entire separation process is completed in a short time, significantly improving the processing efficiency.

[0040] Embodiment 2 By experimenting with the inlet velocity of blood at the spiral inlet 18, the optimal inlet velocity of blood was obtained: 10 m / s. Under the conditions of inlet velocity of 10 m / s and dilution ratio of 1:10, the red blood cell recovery efficiency (RE) was 85.86%, and the concentration efficiency (CE) was 1.275; at inlet velocity of 14 m / s, the red blood cell recovery efficiency (RE) was 93.08%, and the concentration efficiency (CE) was 1.474. Platelet activity and distribution: Under the condition of 10 m / s, the platelet activity reached 78%, and the distribution was mainly concentrated at the top outlet; under the condition of 14 m / s, due to the increase in turbulence intensity, the platelet activity dropped to 41%.

[0041] sample LDH fluorescence value RBC Viability Red blood cell activity damage (RBCDamage) Platelet Luminescent Signal Platelet activity (Viability) 17 m / s overflow 36279.40 17% 83% 55847.91 18% 17 m / s bottom flow 31415.83 53% 47% 34045.48 11% 14 m / s overflow 34688.63 29% 71% 129971.71 41% 14 m / s underflow 32178.80 47% 53% 176445.06 56% 10 m / s overflow 30912.67 56% 44% 246155.05 78% 10 m / s underflow 27523.07 81% 19% 199681.92 63% Summary table of red blood cell and platelet activity in a hydrocyclone (overflow and underflow) at various inlet velocities.

[0042] Embodiment 3 Evaluation of the separation performance of red blood cells: In order to evaluate the separation efficiency of the device, experiments were conducted using whole blood samples with a dilution ratio of 1:10 at different inlet velocities. The experimental results showed two separation rate peaks: 1. At an inlet velocity of 10 m / s, the device achieved a red blood cell recovery efficiency (RE) of 85.86% and a concentration efficiency (CE) of 1.275. At this time, the activities of red blood cells and platelets remained at 81% and 78%, respectively.

[0043] 2. When the inlet velocity was 14 m / s, the recovery efficiency increased to 93.08%, but the cell activity decreased significantly, with the red blood cell activity dropping to 47% and the platelet activity dropping to 41%.

[0044] These data indicate that an inlet velocity of 10 m / s is the optimal operating condition, which can achieve a balance between high separation efficiency and cell viability.

[0045] Embodiment 4 Analysis of the effect of blood type on separation performance: The experiment used stored animal blood, red blood cell suspension and fresh human blood (with different viscosities) to test the separation performance under the conditions of dilution ratio of 1:10 and inlet velocity of 10 m / s: 1. Storage of animal blood: Recovery efficiency (RE) is 90.02%, Concentration efficiency (CE) is 1.337.

[0046] 2. Red blood cell suspension: RE is 86.69%, CE is 1.287.

[0047] 3. Fresh human blood: RE is 87.42%, CE is 1.298.

[0048] The results showed that the device performed consistently when processing different blood samples (including high-viscosity stored blood), and can replace some human samples through animal blood experiments, reducing dependence on fresh human blood.

[0049] Embodiment 5 Turbulence effects and cell viability assessment: The experiment analyzed the effect of turbulence on the activity of red blood cells and platelets: 1. Red blood cell activity (LDH test): At an inlet velocity of 10 m / s, the red blood cell activity is 81%, which drops to 47% after high turbulence conditions (14 m / s).

[0050] 2. Platelet activity (CellTiter-Glo® assay): At an inlet velocity of 10 m / s, platelet activity was 78%, which dropped to 41% at 14 m / s.

[0051] The results showed that turbulence intensity and shear force significantly affected cell activity, and it was recommended that the operating speed be controlled within 10 m / s to protect cell integrity.

[0052] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A micro hydrocyclone for high-throughput blood separation, comprising a mounting base (1), a motor (2) and a plunger pump (3), wherein the output end of the motor (2) is connected to the plunger pump (3), and the motor (2) and the plunger pump (3) are fixedly mounted on the mounting base (1), characterized in that: Also includes: A cyclone separation mechanism, the cyclone separation mechanism is fixedly connected to the mounting base (1), and the cyclone separation mechanism is used to drive the blood to flow and perform centrifugal separation on the blood; a blood conveying mechanism, the blood conveying mechanism being fixedly connected to the mounting base (1), the blood conveying mechanism being in communication with the cyclone separation device, the plunger pump (3) being in communication with the blood conveying mechanism, the blood conveying mechanism being used to convey blood to the cyclone separation mechanism via the plunger pump (3); Wherein, the cyclone separation mechanism is provided with: A conveying assembly, the conveying assembly being fixedly connected to the mounting base (1), the conveying assembly being in communication with the blood separation mechanism, and the conveying assembly being used to guide blood; A cyclone component is fixedly arranged on the conveying component, the cyclone component is connected with the conveying component, and the cyclone component is used for centrifugal separation of blood.

2. A micro hydrocyclone for high-throughput blood separation according to claim 1, characterized in that: The blood delivery mechanism comprises: A storage beaker (4), the storage beaker (4) being fixedly connected to the mounting base (1); A feed pipeline (5), one end of the feed pipeline (5) is connected to the storage beaker (4), and the other end is connected to the liquid inlet of the plunger pump (3); a bypass beaker (6), the bypass beaker (6) being fixedly connected to the mounting base (1); A delivery pipeline (7), wherein the delivery pipeline (7) is respectively connected to the bypass beaker (6), the liquid outlet of the plunger pump (3) and the delivery assembly.

3. A micro hydrocyclone for high-throughput blood separation according to claim 2, characterized in that: A control valve (8) is fixedly connected to one end of the delivery pipeline (7) connected to the bypass beaker (6).

4. A micro hydrocyclone for high-throughput blood separation according to claim 3, characterized in that: The conveying assembly comprises: A fixed frame (9), the fixed frame (9) being fixedly connected to the mounting base (1); A feeding pipe (10), the feeding pipe (10) being fixedly connected to the fixed frame (9), and the two ends of the feeding pipe (10) being respectively connected to the swirl assembly and the conveying pipeline (7); A plasma beaker (11), wherein the plasma beaker (11) is arranged on the mounting base (1); A red blood cell beaker (12), wherein the red blood cell beaker (12) is arranged on the mounting base (1).

5. A micro hydrocyclone for high-throughput blood separation according to claim 4, characterized in that: The swirl assembly comprises: A fixed shell (13), the fixed shell (13) being fixedly connected to the fixed frame (9); A Y-shaped feeding pipe (14), wherein the Y-shaped feeding pipe (14) is fixedly connected to the fixed housing (13), and the Y-shaped feeding pipe (14) is in communication with the feeding pipe (10); A cyclone separator (15), wherein the cyclone separator (15) is fixedly connected to the fixed housing (13), and the cyclone separator (15) is connected to the Y-shaped feed pipe (14).

6. A micro hydrocyclone for high-throughput blood separation according to claim 5, characterized in that: The cyclone separator (15) is provided with: A plasma outlet (16), the plasma outlet (16) being opened at the top of the cyclone separator (15), the plasma outlet (16) being connected to the plasma beaker (11); A red blood cell outlet (17), the red blood cell outlet (17) being disposed at the bottom of the cyclone separator (15), and the red blood cell outlet (17) being connected to the red blood cell beaker (12); A spiral inlet (18), wherein two spiral inlets (18) are provided, and both spiral inlets (18) are fixedly connected to the top of the cyclone separator (15).

7. A micro hydrocyclone for high-throughput blood separation according to claim 6, characterized in that: The two spiral inlets (18) are symmetrically arranged, and both of the two spiral inlets (18) are connected to a side of the Y-shaped feeding tube (14) away from the feeding tube (10).

8. A micro hydrocyclone for high-throughput blood separation according to claim 7, characterized in that: The interior of the cyclone separator (15) is configured as a cylindrical chamber, and the diameter of the internal cylindrical chamber of the cyclone separator (15) is 4-8 mm.

9. A micro hydrocyclone for high-throughput blood separation according to claim 8, characterized in that: The cone portion of the cyclone separator (15) is provided with a cone angle of 2-6°.

10. A micro hydrocyclone for high-throughput blood separation according to claim 9, characterized in that: The insertion angle between the spiral inlet (18) and the cyclone separator (15) is 5-20°.

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