Microorganism detection device and detection method thereof

Abstract

The application relates to the technical field of microorganism detection, in particular to a microorganism detection device and a detection method thereof. The device comprises a culture bin, a bin cover is arranged on the culture bin, a rotating disc is rotatably arranged in the culture bin, a plurality of circumferentially distributed accommodating cavities are arranged on the rotating disc, a culture dish is arranged in the accommodating cavities, the culture dish comprises a base rotatably arranged in the accommodating cavity, a base cover is arranged on the base, a sliding ring is arranged on the base cover, and a sliding ring is slidably arranged in the sliding ring. When the culture dish rotates to the lower side of an optical detector, the sliding ring is located directly below the optical detector, meanwhile, due to the high end of the magnetic ring being located directly below the detector, the tray moves upward, the culture solution and part of the microorganisms are gathered in the sample reservation area, at this time, the optical detector moves downward, the lens is pressed on the sample reservation area, the imaging clarity and the detection accuracy are ensured, the optical detector performs optical imaging on the sample in the sample reservation area, collects imaging data, and detects the microorganisms.

Description

Technical Field

[0001] This application relates to the field of microbial detection technology, and in particular to a microbial detection device and its detection method. Background Technology

[0002] Microbial detection plays a crucial role in food safety, environmental monitoring, medical diagnosis, and biotechnology. With advancements in technology, microbial detection techniques have evolved from traditional culture and microscopic observation methods to modern molecular biology and immunological detection methods. However, the accuracy and efficiency of microbial detection still face numerous challenges.

[0003] To address these challenges, various novel microbial detection devices are constantly being developed to improve detection sensitivity, specificity, automation, and ease of operation. These devices allow for direct observation of the morphology and structure of microorganisms using optical or electron microscopes. While intuitive, this method is difficult to accurately detect in samples with small numbers of microorganisms, necessitating the inoculation of samples onto specific culture media.

[0004] For example, the microbial detection device disclosed in CN115305186A relates to the field of microbial detection technology. This prior art includes an incubator and a detection mechanism. The detection mechanism is located on the inner top surface of the incubator and can move along the length direction of the incubator. A pair of first support plates are spaced apart in the incubator along the width direction. Each first support plate has a plurality of first placement slots evenly spaced along the length direction of the incubator. Each first support plate can move along the width direction of the incubator. A pair of second support plates are spaced apart in the incubator along the width direction. Each second support plate has a plurality of second placement slots evenly spaced along the length direction of the incubator. Each second support plate can move along the width direction of the incubator and can also move in the vertical direction.

[0005] However, the aforementioned existing technologies still have some shortcomings when it comes to microbial sampling and detection:

[0006] The aforementioned existing technology uses fluorescently labeled antibodies to detect microorganisms via nucleic acid probes. Bacteria emit fluorescence under light of a specific wavelength, which can be detected by a fluorescence sensor. During the detection process, the nucleic acid probe comes into direct contact with the microbial sample. After the detection is completed, the probe moves from one petri dish to the next. In this process, microorganisms from the previous petri dish may remain on the probe. If the probe is not adequately cleaned or disinfected, these residual microorganisms will be carried to the next petri dish, leading to cross-contamination. The residual microorganisms may cause false positive results in the samples of the next petri dish, that is, a sample that originally did not contain the target microorganism is mistakenly identified as containing the target microorganism.

[0007] Based on this, as stated above, there is still room for improvement in existing technologies for microbial sampling and detection. Summary of the Invention

[0008] To address the aforementioned technical problems, this application provides a microbial detection device and its detection method, employing the following technical solution:

[0009] In a first aspect, a microbial detection device includes a culture chamber with a cover, a turntable rotatably disposed inside the culture chamber, a plurality of circumferentially distributed receiving cavities constructed on the turntable, a detachable culture dish disposed inside the receiving cavity, and a detector corresponding to the culture dish disposed on the cover.

[0010] The culture dish includes a base that is rotatably disposed within a cavity, and the base is detachable. A base cover is disposed on the base cover, and a sliding ring is disposed on the base cover, with the lower end of the sliding ring penetrating through the base cover. A sliding ring is slidably disposed inside the sliding ring, and a lens is disposed at the bottom of the sliding ring.

[0011] Preferably, the petri dish further includes a tray that slides within the base, the tray having a sample retention area located directly below the lens, and the tray having several flow holes located outside the sample retention area.

[0012] Preferably, the tray is magnetic, and a magnetic ring is provided at the bottom of the cultivation chamber below the turntable. The upper end of the magnetic ring is constructed with an inclined surface, and the magnetism of the magnetic ring is repulsive to the magnetism of the tray.

[0013] Preferably, the bottom of the base is provided with protrusions that correspond one-to-one with the flow holes.

[0014] Preferably, the base has several circumferentially evenly distributed sliding grooves, the base cover is rotatably connected to the base, and a removable sampling bottle is slidably disposed in the sliding groove.

[0015] Preferably, a sampling port is provided in the sliding groove, and a connecting ring corresponding to the sampling port is rotatably provided in the base, and the connecting ring has a break corresponding to the sampling port.

[0016] Preferably, the connecting ring is provided with a locking block, and the bottom of the base cover is provided with a locking groove corresponding to the locking block.

[0017] Preferably, the sampling bottle has an inlet corresponding to the sampling port.

[0018] Preferably, the detector includes a protective cover on the cover corresponding to the receiving cavity, a chassis inside the protective cover, and an optical detector slidably mounted on the chassis, located directly above the lens.

[0019] Secondly, a method for detecting microorganisms, the method of use of which includes the following steps:

[0020] S1: Culture treatment. In microbial testing, the petri dish is sterilized and filled with culture medium. The bacteria to be tested are then placed into the culture medium, and the top cover is closed.

[0021] S2: When the mixture moves upward and the rotating disk is driven, the culture dish slides to the high end, the magnetic ring and the tray repel each other and increase, causing the tray to move upward in the base, and the culture medium flows through the flow hole into the bottom of the tray;

[0022] S3: Mixing and moving downwards, when the petri dish is rotated to the lower end, the magnetic ring and the tray repel each other less, the tray descends, and the culture medium passes through the flow hole into the upper part of the tray to mix with the microorganisms;

[0023] S4: Detection preparation. The flow hole is located outside the sample retention area. Microorganisms are suspended on the surface of the culture medium, and some are gathered in the sample retention area, which is located below the lens.

[0024] S5: Optical detection. The optical detector moves down and the lens presses on the sample retention area. The optical detector performs optical imaging on the sample in the sample retention area 451 and collects imaging data to detect microorganisms.

[0025] In summary, this application includes at least the following beneficial technical effects:

[0026] This invention achieves automatic collection and separation of some microorganisms in the culture medium by rotating the petri dish and raising the magnetic tray. The optical detector can clearly image and detect the samples in the sample retention area. At the same time, by rotating the base cover and magnetic ring, precise sampling of microorganisms is achieved. The detection probe is used to detect the samples in the sampling bottle, avoiding direct contact with microorganisms in the petri dish, thereby minimizing the risk of contamination. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the structure of the present invention.

[0028] Figure 2 This is a cross-sectional view of the present invention.

[0029] Figure 3 This is a schematic diagram of the structure between the cultivation chamber and the turntable of the present invention.

[0030] Figure 4 This is a schematic diagram of the structure of the petri dish of the present invention.

[0031] Figure 5 This is a cross-sectional view of the base cover of the present invention.

[0032] Figure 6 This is a schematic diagram of the structure of the tray of the present invention.

[0033] Figure 7 This is a schematic diagram of the structure of the magnetic ring of the present invention.

[0034] Figure 8 This is a cross-sectional view of the petri dish of the present invention.

[0035] Figure 9 This is a schematic diagram of the structure between the base and the connecting ring of the present invention.

[0036] Figure 10 This is a schematic diagram of the connecting ring of the present invention.

[0037] Figure 11 This is a schematic diagram of the sampling bottle of the present invention.

[0038] Figure 12 This is a schematic diagram of the detector structure of the present invention.

[0039] Figure 13 This is a schematic diagram of the structure of the driving component of the present invention.

[0040] Figure 14 This is a schematic diagram of the structure between the baffle and the reset spring of the present invention.

[0041] Explanation of reference numerals in the attached diagram: 1. Incubation chamber; 11. Chamber cover; 2. Turntable; 21. Receiving cavity; 3. Top cover; 4. Petri dish; 41. Base; 42. Base cover; 43. Slip ring; 44. Sliding ring; 441. Lens; 45. Tray; 451. Sample retention area; 452. Flow hole; 46. Magnetic ring; 47. Sloping surface; 471. High end; 472. Low end; 48. Protrusion; 49. Sliding groove; 491. Notch; 5. Sampling bottle; 51. Sampling port; 52. 53. Connecting ring; 54. Break; 55. Locking block; 56. Locking slot; 57. Sample inlet; 68. Rubber plug; 69. Detector; 60. Protective cover; 61. Chassis; 62. Optical detector; 73. Drive component; 74. Drive shaft; 75. Ratchet 1; 76. Pawl 1; 77. Gear ring; 78. Transmission gear; 79. Ratchet 2; 70. Pawl 2; 81. Detection probe; 82. Sealing plate; 83. Magnetic strip; 84. Baffle; 85. Return spring; 86. Stage. Detailed Implementation

[0042] The following is in conjunction with the appendix Figures 1 to 14 This application will be described in further detail.

[0043] This application discloses a microbial detection device and its detection method. It avoids direct contact with microorganisms through optical detection, and at the same time, it uses a method of isolating a portion of the culture medium to prevent the probe from directly contacting the sample, thus avoiding the risk of contamination.

[0044] Example 1:

[0045] Reference Figure 1, Figure 2 and Figure 3 As shown, a microbial detection device includes a culture chamber 1, which is constructed as a sealed circular container with a cover 11 on its top. The cover 11 is also constructed as a circular structure and fits tightly with the culture chamber 1 to ensure a stable internal environment.

[0046] The culture chamber 1 is equipped with a turntable 2. The turntable 2 is circular and is installed in the culture chamber 1 by bearings so that it can rotate. Multiple receiving cavities 21 are evenly distributed on the turntable 2. Removable culture dishes 4 are set in the receiving cavities 21. The receiving cavities 21 are used to place the culture dishes 4. The receiving cavities 21 are designed to be annular, with an inner diameter slightly larger than the diameter of the culture dishes 4 to ensure that the culture dishes 4 are placed stably.

[0047] In this embodiment, three sets of receiving cavities 21 are preferably arranged in a uniform 120-degree pattern to facilitate the simultaneous execution of multiple sets of experiments. The culture dishes 4 are also preferably set in three sets, corresponding one-to-one with the receiving cavities 21. However, this application does not limit the number of receiving cavities 21 and culture dishes 4, and can be flexibly adjusted according to actual needs to adapt to detection tasks of different scales.

[0048] When testing for microorganisms, first disinfect petri dish 4, then disinfect petri dish 4 and inject culture medium into petri dish 4, and then put the bacterial sample to be tested into the culture medium.

[0049] Because of the close fit between the culture dish 4 and the receiving cavity 21, the stability and sealing of the culture medium are ensured, and external contamination is avoided. Then, the top cover 3 on the cover 11 corresponding to the receiving cavity 21 is opened, and the culture dish 4 is placed into the receiving cavity 21.

[0050] Then, the drive unit 7 between the incubation chamber 1 and the chamber cover 11 is activated, which drives the turntable 2 to rotate. The turntable 2 rotates smoothly under the action of the drive unit 7, so that the subsequent receiving chamber 21 moves to the bottom of the top cover 3, thereby placing multiple culture dishes 4 into the receiving chamber 21 of the turntable 2. Then, the top cover 3 is closed, and it is ensured that the top cover 3 is tightly closed to maintain the internal sterile environment. The top cover 3 is made of transparent material to facilitate observation of the internal situation.

[0051] In summary, the microbial detection device of this application achieves efficient detection of multiple samples by precisely controlling the rotation of the turntable 2, while ensuring stable culture of samples in a sterile environment, avoiding cross-contamination, and improving detection accuracy and efficiency. Secondly, this application can realize the synchronous transfer of multiple culture dishes 4, with outstanding continuous detection capability, greatly shortening the detection cycle and optimizing the experimental process.

[0052] Furthermore, this application allows for the replacement and assembly of one or more sets of culture dishes 4, meaning that multiple culture dishes 4 can simultaneously detect multiple microorganisms or perform multiple sets of control tests on one microorganism, thereby meeting diverse experimental needs and enhancing the flexibility and practicality of the device; in addition, the transparent top cover 3 facilitates real-time observation of the distribution of microorganisms.

[0053] During the cultivation process, the detector 6 installed on the cover 11 can detect the microorganisms in the corresponding culture dish 4. The detector 6 achieves non-contact detection through optical imaging to prevent external contamination.

[0054] Meanwhile, when needed, the drive unit 7 drives the turntable 2 to rotate, causing the culture dish 4 on the turntable 2 to rotate as well. Centrifugal force can be used to fully mix the microorganisms in the culture dish 4 with the culture medium, ensuring the uniformity of the sample and the accuracy of the test results.

[0055] Based on this, this application uses a set of detectors 6 to detect multiple culture dishes 4 on the culture chamber 1 one by one. Specifically, the drive unit 7 is used to drive the turntable 2 to rotate, so that each culture dish 4 is aligned with the detector 6 in turn, thereby achieving automated and high-precision continuous detection.

[0056] Reference Figure 4 , Figure 5 and Figure 6 As shown, specifically, the petri dish 4 includes a base 41 that is rotatably disposed within the receiving cavity 21, and the base 41 is detachable, which facilitates the replacement and cleaning of the base 41 and ensures the cleanliness and reproducibility of the experiment.

[0057] A base cover 42 is rotatably mounted on the base 41. Both the base 41 and the base cover 42 are constructed in a circular shape to facilitate fitting with the receiving cavity 21 and ensure stable rotation. At the same time, the base 41 and the base cover 42 are tightly connected by a sealing ring to prevent leakage of the culture medium and ensure that the sample remains sterile during rotation. The sealing ring is made of a soft and corrosion-resistant material to extend its service life and ensure long-term stable operation.

[0058] Furthermore, both the base 41 and the base cover 42 are made of high-temperature resistant materials to ensure the high-temperature sterilization effect.

[0059] Specifically, when detecting microorganisms, this application first disinfects the base 41 and the base cover 42, injects culture medium into the base 41, then places the sample into the culture medium, covers the base cover 42, and ensures that the sealing ring fits tightly to prevent leakage of the culture medium. Subsequently, the top cover 3 on the compartment cover 11, which corresponds to the receiving cavity 21, is opened, the culture dish 4 is placed into the receiving cavity 21, and the top cover 3 is closed.

[0060] The above process completes the disinfection of petri dish 4, the injection of culture medium, and the placement of samples, ensuring the sterility of the testing environment.

[0061] Furthermore, a slip ring 43 is provided on the base cover 42, with the lower end of the slip ring 43 extending to the lower end of the base cover 42. The slip ring 43 is also constructed as a circular structure and is coaxially arranged with the base 41 to ensure synchronization during rotation. A slip ring 44 is slidably arranged inside the slip ring 43, and the slip ring 44 is constructed as a circular structure coaxial with the slip ring 43. The slip ring 44 and the slip ring 43 are connected by a spring (not shown in the figure), so that the slip ring 44 always maintains the potential energy for upward movement.

[0062] A transparent lens 441 is provided at the bottom of the sliding ring 44, which facilitates non-contact detection by the detector 6 and prevents external contamination. The lens 441 is made of a high-transmittance material to ensure the clarity of the detection signal.

[0063] Through the above design, this application achieves non-contact detection using a high-transmittance lens 441, avoiding sample contamination and improving detection efficiency and accuracy.

[0064] Meanwhile, the surface of lens 441 is specially treated to have an anti-fog function, ensuring a clear field of view under any temperature and humidity conditions, further guaranteeing the reliability of the test results.

[0065] Then, in some existing technologies, microbial samples are placed in petri dishes 4 and cultured in an incubator. The microbial samples are in a relatively static state in the incubator. During the culture process, bacteria or other microorganisms may form precipitates or aggregates in the culture medium. When performing colony counting or turbidity measurement, the precipitated or aggregated samples cannot provide accurate results.

[0066] This application uses a rotating base 41 and a base cover 42 to maintain a uniform distribution of microbial samples in the culture medium, avoiding precipitation or aggregation. At the same time, the centrifugal force of the turntable 2 ensures that the samples are uniformly mixed during rotation, further preventing precipitation or aggregation, thus improving the accuracy and reliability of sample detection and effectively solving the problem of uneven sample distribution in traditional culture methods.

[0067] However, simply rotating the sample cannot completely eliminate the sediment. This application further optimizes the design of culture dish 4, specifically:

[0068] Reference Figure 6 , Figure 7 and Figure 8 As shown, the petri dish 4 also includes a tray 45 that is slidably disposed within the base 41. The tray 45 is a disc structure with a raised edge on its outer edge. A sample retention area 451 is provided on the tray 45. The sample retention area 451 is located at the center of the tray 45 and directly below the lens 441, which facilitates accurate detection.

[0069] Furthermore, the tray 45 is magnetic, and a magnetic ring 46 is provided at the bottom of the cultivation chamber 1 below the turntable 2. The magnetic field generated by the magnetic ring 46 interacts with the magnetism of the tray 45, resulting in a repulsive force between the two.

[0070] The upper surface of the magnetic ring 46 is constructed as a slope 47, which is constructed as a high end 471 and a low end 472. The high end 471 is located directly below the detector 6, and the low end 472 is far away from the detector 6.

[0071] Specific work process:

[0072] During the process of the drive component 7 driving the turntable 2, when the culture dish 4 slides to the high end 471 of the magnetic ring 46, the magnetic ring 46 and the tray 45 repel each other due to their magnetism, and the repulsive force is at its maximum at this time. The tray 45 is pushed upward. At this time, the culture medium at the top of the tray 45 will flow into the bottom of the tray 45 through several flow holes 452 opened on the tray 45. At the same time, the sample retention area 451 is closer to the lens 441, which facilitates the detector 6 to perform accurate detection. It can be seen that the tray 45 is lifted under the action of magnetic force, and the culture medium is dynamically circulated to ensure uniform sample distribution and avoid precipitation.

[0073] Conversely, when the driving component 7 drives the petri dish 4 to rotate to the lower end 472, the tray 45 gradually descends due to the weakening of the magnetic force. Under its own gravity, the tray 45 falls back to its original position, and the culture medium flows back to the top of the tray, forming a circulating flow. This ensures continuous mixing of the sample and further prevents precipitation. It can be seen that by moving the tray 45 up and down during the rotation process, the dynamic circulation of the culture medium is achieved, which not only ensures the uniform distribution of the sample but also improves the accuracy of the detection, completely solving the problem of sample precipitation in traditional culture methods.

[0074] The ingeniously designed flow hole 452 of the tray 45 ensures smooth liquid flow and avoids blockage; the magnetic field strength of the magnetic ring 46 is adjustable to adapt to different sample requirements.

[0075] The tray 45 is made of corrosion-resistant and biocompatible materials to ensure that it will not contaminate samples during long-term use.

[0076] In addition, it should be noted that several flow holes 452 are located around the sample retention area 451 in a ring-shaped distribution. Most microorganisms will be suspended on the surface of the culture medium, while some of the culture medium and some microorganisms will gather in the sample retention area 451. This ensures the stability of the central sample in the sample retention area 451 and promotes the circulation of the surrounding culture medium, further optimizing the uniformity of sample distribution and improving the reliability of the test results.

[0077] The annular distribution design of the flow holes 452 effectively guides the culture medium to form a vortex within the tray 45, enhancing the mixing effect and ensuring that the sample is distributed without dead corners.

[0078] The sample retention area 451 is located directly below the lens 441. The detector 6 uses optical imaging technology to perform non-contact detection of microorganisms in the sample retention area 451 through the lens 441, thus avoiding sample contamination and cross-infection.

[0079] Since the sample retention area 451 is located in the middle of the tray 45, when the culture medium flows from the flow hole 452, the culture medium in the sample retention area 451 will gradually diffuse to the surrounding area to form a dynamic equilibrium, but will always retain the sample at the top of the culture medium, ensuring that the detector 6 captures the most representative sample data, thus improving the accuracy and reliability of the detection results.

[0080] In summary, the dynamic circulation design of tray 45, combined with optical imaging technology, enables efficient and pollution-free microbial detection.

[0081] Furthermore, the bottom of the base 41 is provided with protrusions 48 that correspond one-to-one with the flow holes 452. When the tray 45 moves to the bottom of the base 41, the protrusions 48 will be embedded in the flow holes 452, so that no culture medium remains in the flow holes 452. This allows the culture medium at the bottom of the tray 45 to completely enter the top of the tray 45, achieving complete transfer of the culture medium. This completely avoids culture medium residue, ensures thorough mixing of the sample and the culture medium, and improves the uniformity and accuracy of the detection.

[0082] In addition, the material used for the base 41 has high wear resistance and chemical stability, ensuring that it will not deform during long-term use, further improving the overall stability and service life of the equipment; and the precise fit between the tray 45 and the base 41, together with the flexible adjustment of the magnetic ring 46, creates an efficient and reliable microbial detection environment.

[0083] The protrusion 48 at the bottom of the base 41 not only ensures complete transfer of the culture medium, but also facilitates quick cleaning and disinfection, reducing maintenance time.

[0084] Reference Figure 8 , Figure 9 and Figure 10 As shown, specifically, the detector 6 includes a protective cover 61 on the cover 11 corresponding to the receiving cavity 21. The protective cover 61 is constructed as a hollow cylindrical shell structure, which can effectively isolate external environmental interference and ensure that the detection process is not affected by external factors. A chassis 62 is provided inside the protective cover 61, and an optical detector 63 located directly above the lens 441 is slidably mounted on the chassis 62.

[0085] The optical detector 63 preferably uses a high-resolution imaging type, which captures microbial images through a high-precision sensor, enabling it to capture minute microbial details, accurately identify microbial characteristics, and improve the accuracy of detection results.

[0086] When the petri dish 4 is rotated to the underside of the optical detector 63, the sliding ring 44 will be directly below the optical detector 63. At the same time, since the high end 471 of the magnetic ring 46 is directly below the detector 6, the tray 45 will move upward, and the culture medium and some microorganisms will gather in the sample retention area 451. At this time, the optical detector 63 moves downward, causing the sliding ring 44 to move downward and compress the spring. The lens of the optical detector 63 is pressed against the lens 441 to ensure that the sample image is captured without blind spots. The lens 441 will press on the sample retention area 451, so that the sample image in the sample retention area 451 is clearly presented.

[0087] The optical detector 63 captures every detail of the sample through a high-precision sensor and collects imaging data to detect microorganisms. At the same time, the automatic focusing function equipped in the optical detector 63 ensures that the image remains clear under different sample thicknesses, further improving detection efficiency and accuracy.

[0088] In addition, in some existing technologies, such as those described in the background section, the detection of microorganisms requires frequent sampling using nucleic acid probes. External contaminants may be introduced during the sampling process, affecting the accuracy of the measurement results.

[0089] To solve this problem, this application provides a plurality of circumferentially evenly distributed sliding grooves 49 on the base 41. The sliding grooves 49 are distributed on the outer side wall of the base 41. The base cover 42 is rotatably connected to the base 41. The base cover 42 has a notch 491 corresponding to the sliding groove 49. A detachable sampling bottle 5 is slidably disposed in the sliding groove 49.

[0090] By aligning the notch 491 with one of the sliding grooves 49, the sampling bottle 5 can be slid into the sliding groove 49 from the notch 491 for installation, or the sampling bottle 5 in the sliding groove 49 can be slid out from the notch 491 for disassembly. The design of the sampling bottle 5 makes it tightly integrated with the base 41, that is, the concave surface of the sampling bottle 5 is tightly fitted with the convex surface of the base 41 to ensure no gaps and prevent external contaminants from entering. The convex surface of the sampling bottle 5 is in close contact with the inner wall of the receiving cavity 21.

[0091] When the notch 491 rotates between the two sliding grooves 49, it can prevent the sampling bottle 5 from disengaging from the sliding grooves 49. The installation and removal process of the sampling bottle 5 is simple and efficient, effectively avoiding the risk of contamination caused by frequent sampling.

[0092] Specifically, refer to Figure 10 , Figure 11 and Figure 12As shown, a sampling port 51 is provided in the sliding groove 49. The sampling port 51 is located in the upper region of the sliding groove 49. A connecting ring 52 corresponding to the sampling port 51 is rotatably provided in the base 41. The connecting ring 52 is a ring structure, and its longitudinal length is greater than the depth of the sampling port 51, ensuring that the sampling bottle 5 and the connecting ring 52 are tightly connected.

[0093] The connecting ring 52 is provided with a locking block 54. When the base cover 42 is placed on the base 41, the locking groove 55 at the bottom of the base cover 42 will engage with the locking block 54. The locking block 54 of the connecting ring 52 and the locking groove 55 of the base cover 42 are tightly engaged to ensure that the base cover 42 is securely locked and prevents accidental opening.

[0094] The sampling bottle 5 is seamlessly connected to the connecting ring 52 through the sampling port 51, which further enhances the sealing of the device and effectively isolates external contamination. The concave and convex design of the sampling bottle 5 makes it fit tightly against the inner wall of the base 41 and the receiving cavity 21, forming multiple protective barriers to ensure the purity of the sample during the detection process, thereby greatly improving the reliability of the detection results.

[0095] In addition, the sampling bottle 5 is made of highly corrosion-resistant material to ensure stable use in different chemical environments and extend its service life.

[0096] When sampling microorganisms in the petri dish 4, rotating the base cap 42 can drive the connecting ring 52 to rotate, so that the cut 53 on the connecting ring 52 corresponds to one of the sampling ports 51. Then, the driving component 7 drives the petri dish 4 to rotate to the high end 471 of the magnetic ring 46, thereby causing the tray 45 to lift the culture medium. Part of the culture medium will fall into the bottom of the tray 45 through the flow hole 452, and another part of the culture medium will pass through the cut 53 through the sampling port 51 and enter the sampling bottle 5 through the inlet 56 on the sampling bottle 5 corresponding to the sampling port 51.

[0097] The inlet 56 of the sampling bottle 5 is precisely aligned with the sampling port 51 to ensure smooth flow of the culture medium and avoid overflow or contamination. At the same time, the sealing design of the inlet 56 effectively prevents air from entering, maintains the original state of the sample, and improves the accuracy of the test data.

[0098] The protective cover 61 is rotatably connected to the compartment cover 11. A detection probe 8 is slidably mounted on the chassis 62, positioned directly above the sampling bottle 5. The protective cover 61 is rotated by the drive component 7, moving the detection probe 8 to the upper end of the sampling bottle 5. This allows the detection probe 8 to be precisely positioned and vertically pierce the rubber stopper 57 at the upper end of the sampling bottle 5, detecting the microbial sample inside. The precise insertion of the detection probe 8 prevents sample leakage, ensuring a sterile environment during the testing process. The elastic design of the rubber stopper 57 allows it to quickly return to a sealed state after testing, further ensuring the integrity of the sample.

[0099] In addition, the rubber stopper 57 is detachably mounted on the sampling bottle 5, so that the detection probe 8 does not come into direct contact with the microorganisms in the culture dish 4, avoiding cross-contamination. At the same time, multiple sampling bottles 5 can be sampled and tested at different times. After the test is completed, the detection probe 8 is retracted. This design not only improves the detection efficiency, but also ensures the accuracy and reliability of the data.

[0100] The detection probe 8 is made of high-precision stainless steel, which is highly corrosion-resistant and ensures stable operation in different environments. The protective cover 61 is designed to effectively isolate external interference, ensuring that the detection process is not affected by external factors.

[0101] Furthermore, refer to Figure 13 As shown, the driving component 7 includes a drive shaft 71 that rotatably passes through the culture chamber 1. The upper end of the drive shaft 71 rotatably passes through the turntable 2. A ratchet 72 is provided on the drive shaft 71, and a pawl 73 that engages with the ratchet 72 is provided on the turntable 2. When the drive shaft 71 rotates in the forward direction, the pawl 73 drives the turntable 2 to rotate, achieving precise positioning of the culture dish 4. When the drive shaft 71 rotates in the forward direction, the pawl 73 separates from the ratchet 72 and cannot drive the turntable 2 to rotate in the reverse direction, ensuring the stable position of the culture dish 4.

[0102] Looking back Figure 9 As shown, the protective cover 61 is rotatably connected to the compartment cover 11, and the compartment cover 11 is provided with a gear ring 74. A transmission gear 75 that meshes with the gear ring 74 is rotatably mounted on the compartment cover 11. A ratchet 76 is rotatably mounted on the upper end of the drive shaft 71 and extends to the upper end of the compartment cover 11. A pawl 77 that meshes with the ratchet 76 is mounted on the transmission gear 75. When the drive shaft 71 rotates in the forward direction, the pawl 77 will not drive the transmission gear 75 to rotate. When it rotates in the reverse direction, the pawl 77 meshes with the ratchet 76 and drives the transmission gear 75 to rotate.

[0103] The design of ratchet 2 76 ensures the stability of transmission gear 75 when rotating in the opposite direction, making the operation of the entire detection device more flexible and efficient.

[0104] The drive shaft 71 is supported by bearings to ensure smooth rotation and reduce friction loss. The lower end of the drive shaft 71 is connected to a motor drive device. After the motor starts, it drives the drive shaft 71 to rotate precisely, realizing the coordinated operation of various components and ensuring the accuracy and efficiency of the testing process.

[0105] The motor is preferably a high-precision stepper motor, which has low noise and high torque characteristics, ensuring that the drive shaft 71 can maintain precise control even when running at high speed.

[0106] The specific process is as follows:

[0107] The drive shaft 71 is driven to rotate forward by the motor. The rotating drive shaft 71 will drive the ratchet 72 to rotate together. The rotating ratchet 72 drives the pawl 73, which in turn pushes the turntable 2 to rotate, so as to achieve precise positioning of the petri dish 4. At this time, the pawl 77 separates from the ratchet 76 to ensure that the transmission gear 75 does not interfere with the positioning process.

[0108] When the drive shaft 71 is reversed by the motor, the second pawl 77 engages with the second ratchet 76, driving the transmission gear 75 to rotate. The protective cover 61 rotates accordingly, causing the protective cover 61 to move the base 62, which in turn moves the detection probe 8 to the upper end of the sampling bottle 5 to be tested. This ensures the detection probe 8 is precisely aligned with the sampling bottle 5, guaranteeing accurate sampling, preventing sample contamination, and improving overall testing efficiency and accuracy. Meanwhile, the first ratchet 72 separates from the first pawl 73, preventing accidental movement of the turntable 2 and ensuring the petri dish 4 remains in a fixed position during testing, further guaranteeing the accuracy and reliability of the test data.

[0109] The forward and reverse design of the drive shaft 71, combined with a high-precision stepper motor, enables efficient switching and precise control of the detection device, ensuring seamless connection of each detection link and improving the stability and reliability of the overall detection process.

[0110] Example 2:

[0111] Reference Figure 12 , Figure 13 and Figure 14 As shown, based on Example 1, after the sampling bottle 5 is removed, the rotation of the connecting ring 52 will cause the break 53 to connect with the open sampling port 51, causing external contaminants to enter the petri dish 4. At the same time, the inlet 56 of the removed sampling bottle 5 will open, and microorganisms will enter the sampling bottle 5.

[0112] To prevent contamination, in this embodiment, a sealing plate 81 is hinged inside the injection port 56 by a torsion spring (not shown in the figure). The sealing plate 81 is magnetic, and magnetic strips 82 are provided on both sides of the break 53 of the connecting ring 52. The magnetism of the magnetic strips 82 is repulsive to the magnetism of the sealing plate 81.

[0113] When the break 53 is aligned with the sampling port 51, the magnetic repulsion between the magnetic stripe 82 and the sealing plate 81 forces the sealing plate 81 to rotate and compresses the torsion spring. After a portion of the culture medium passes through the sampling port 51 from the break 53 and enters the sampling bottle 5 through the inlet 56 corresponding to the sampling port 51, the base cap 42 is rotated so that the break 53 of the connecting ring 52 is located between the two sliding grooves 49, so that the break 53 and the sampling port 51 are misaligned, thus achieving closure.

[0114] At this point, the compressed torsion spring will push the sealing plate 81 to block the sample inlet 56, preventing microorganisms from entering and ensuring the purity of the sample. The design of the sealing plate 81 cleverly utilizes the principle of magnetic repulsion, effectively avoiding the risk of contamination and further improving the safety and reliability of the detection device.

[0115] A baffle 83 is slidably installed inside the sliding groove 49. When the break 53 is misaligned, the baffle 83 automatically slides into the closed position to further strengthen the sealing effect and ensure that no external contaminants can enter. A return spring 84 located at the lower end of the baffle 83 is slidably installed inside the sliding groove 49. The sampling bottle 5 is provided with a step 85 corresponding to the baffle 83 and the spring.

[0116] By aligning the notch 491 with one of the sliding grooves 49, the sampling bottle 5 can be slid into the sliding groove 49 through the notch 491 for installation. The step 85 on the sampling bottle 5 will abut against the baffle 83, forcing the baffle 83 and the return spring 84 to compress, ensuring that the sampling bottle 5 is firmly embedded in the sliding groove 49, so that the sampling port 51 and the inlet port 56 are aligned, achieving a tight connection between the sampling bottle and the detection device, and ensuring the accuracy of the sampling process.

[0117] Conversely, when the sampling bottle 5 in the sliding groove 49 is slid out of the notch 491 for disassembly, the compressed return spring 84 will push the baffle 83 to block the sampling port 51, ensuring that the inside of the petri dish 4 is not contaminated.

[0118] In summary, this application effectively isolates external contaminants through multiple sealing designs and the principle of magnetic repulsion, ensuring sample purity, improving the overall performance and ease of operation of the detection device, and ensuring the accuracy and reliability of experimental results.

[0119] Finally, the present invention also provides a method for detecting microorganisms, the method of use of which includes the following steps:

[0120] S1: Cultivation treatment. When detecting microorganisms, first disinfect the base 41 and the base cover 42, inject culture medium into the base 41, then put the bacteria to be detected into the culture medium and cover the base cover 42. After that, open the top cover 3 on the compartment cover 11 that corresponds to the receiving cavity 21, and put the culture dish 4 into the receiving cavity 21.

[0121] S2: Cultivation preparation. The drive unit 7 between the cultivation chamber 1 and the chamber cover 11 can drive the turntable 2 to rotate, so that the subsequent receiving cavity 21 moves to the bottom of the top cover 3, thereby placing multiple culture dishes 4 into the receiving cavity 21 of the turntable 2, and then closing the top cover 3.

[0122] S3: Mixing and moving upward. During the process of the drive unit 7 driving the turntable 2, due to the magnetic repulsion between the magnetic ring 46 and the magnetic repulsion between the tray 45, when the culture dish 4 slides to the high end 471 of the magnetic ring 46, the magnetic repulsion between the magnetic ring 46 and the tray 45 gradually increases, causing the tray 45 to move upward in the base 41. At this time, the culture medium at the top of the tray 45 will flow into the bottom of the tray 45 through several flow holes 452 opened on the tray 45.

[0123] S4: Mixing and moving downwards. When the driving component 7 drives the petri dish 4 to rotate to the lower end 472, the magnetic repulsion between the magnetic ring 46 and the tray 45 gradually decreases. The tray 45 falls naturally under its own weight. The culture medium below the tray 45 will pass through the flow hole 452 and enter the upper part of the tray 45. Through the up and down movement of the tray 45 during the rotation, the culture medium and microorganisms are triggered to mix.

[0124] S5: Detection preparation. Several flow holes 452 are located outside the sample retention area 451, and most microorganisms will be suspended on the surface of the culture medium. A portion of the culture medium and some microorganisms will be gathered in the sample retention area 451, which is located directly below the lens 441.

[0125] S6: Optical detection. The optical detector 63 moves downward, causing the sliding ring 44 to move downward and compress the spring. The lens 441 will press on the sample retention area 451 to ensure the clarity of the image and the accuracy of the detection. The optical detector 63 performs optical imaging on the sample in the sample retention area 451 and collects imaging data to detect microorganisms.

[0126] S7: Sampling preparation. When sampling microorganisms in the petri dish 4, rotating the base cap 42 can drive the connecting ring 52 to rotate, so that the cut 53 on the connecting ring 52 corresponds to one of the sampling ports 51. Then, the driving component 7 drives the petri dish 4 to rotate to the high end 471 of the magnetic ring 46, so that the tray 45 lifts the culture medium. Part of the culture medium will fall into the bottom of the tray 45 through the flow hole 452, and another part of the culture medium will pass through the cut 53 through the sampling port 51 and enter the sampling bottle 5 from the inlet 56 on the sampling bottle 5 corresponding to the sampling port 51.

[0127] S8: Sampling and testing. The detection probe 8 will puncture the rubber stopper 57 at the top of the sampling bottle 5 to test the microbial sample in the sampling bottle 5. The rubber stopper 57 is detachably mounted on the sampling bottle 5 so that the detection probe 8 does not come into direct contact with the microorganisms in the petri dish 4. The detection probe 8 does not come into direct contact with the sample, thus avoiding the risk of contamination.

[0128] The embodiments described herein are preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made in accordance with the structure, shape, and principle of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A microbial detection device, comprising a culture chamber, wherein the culture chamber is provided with a cover, characterized in that: The culture chamber is equipped with a rotating turntable, which has multiple circumferentially distributed cavities. Each cavity contains a detachable culture dish, and the chamber cover is equipped with a detector corresponding to the culture dish. The culture dish includes a base that is rotatably disposed within a cavity, and the base is detachable. A base cover is disposed on the base cover, and a sliding ring is disposed on the base cover, with the lower end of the sliding ring penetrating through the base cover. A sliding ring is slidably disposed inside the sliding ring, and a lens is disposed at the bottom of the sliding ring. The petri dish also includes a tray that slides inside the base, and a sample retention area located directly below the lens is provided on the tray. The tray has several flow holes located outside the sample retention area. The tray is magnetic, and a magnetic ring is provided at the bottom of the cultivation chamber below the turntable. The upper end of the magnetic ring is constructed with an inclined surface, and the magnetism of the magnetic ring is repulsive to the magnetism of the tray. The base has several circumferentially evenly distributed sliding grooves, the base cover is rotatably connected to the base, and a detachable sampling bottle is slidably disposed in the sliding groove. A sampling port is provided in the sliding groove, and a connecting ring corresponding to the sampling port is rotatably provided in the base. The connecting ring has a break corresponding to the sampling port. The connecting ring is provided with a locking block, and the bottom of the base cover is provided with a locking groove corresponding to the locking block; The sampling bottle has an inlet corresponding to the sampling port.

2. The microbial detection device according to claim 1, characterized in that: The bottom of the base is provided with protrusions that correspond one-to-one with the flow holes.

3. The microbial detection device according to claim 1, characterized in that: The detector includes a protective cover on the cover corresponding to the receiving cavity, a chassis inside the protective cover, and an optical detector slidably mounted on the chassis, located directly above the lens.

4. A method for detecting microorganisms for purposes other than disease diagnosis or treatment, employing a microorganism detection device as described in any one of claims 1-3, characterized in that, Its usage includes the following steps: S1: Culture treatment. In microbial testing, the petri dish is sterilized and filled with culture medium. The bacteria to be tested are then placed into the culture medium, and the top cover is closed. S2: When the mixture moves upward and the rotating disk is driven, the culture dish slides to the high end, the magnetic ring and the tray repel each other and increase, causing the tray to move upward in the base, and the culture medium flows through the flow hole into the bottom of the tray; S3: Mixing and moving downwards, when the petri dish is rotated to the lower end, the magnetic ring and the tray repel each other less, the tray descends, and the culture medium passes through the flow hole into the upper part of the tray to mix with the microorganisms; S4: Detection preparation. The flow hole is located outside the sample retention area. Microorganisms are suspended on the surface of the culture medium, and some are gathered in the sample retention area, which is located below the lens. S5: Optical detection. The optical detector moves down and the lens presses on the sample retention area. The optical detector performs optical imaging on the sample in the sample retention area and collects imaging data to detect microorganisms.

Images