Algae-inhibiting compound based on invasive plants as well as preparation method and application of algae-inhibiting compound

Abstract

The invention relates to the technical field of water environment governance, and provides an algal inhibition compound based on invasive plants and a preparation method and application thereof.The algal inhibition compound comprises an extract of invasive plant powder and a complexing agent, and the invasive plant powder comprises powder of spartina alterniflora, bidens pilosa, fragrant eupatorium herb and guayule. The complexing agent comprises ferric citrate, ethylenediamine tetraacetic acid disodium salt and phytic acid; the invention further provides a preparation method of the algal inhibition compound based on the invasive plants and application of the algal inhibition compound in removal of single algae or removal of mixed algae in rivers / lakes / reservoirs / ponds / landscape pools. According to the algal inhibition compound based on the invasive plant, the invasive plant is converted into a high-added-value algal inhibition preparation, the algal inhibition effect is improved through the synergistic effect of plant active ingredients and the complexing agent, and the release period of the algal inhibition compound is prolonged.

Description

Technical Field

[0001] The present invention relates to the technical field of water environment treatment, and in particular to an anti-algae compound based on invasive plants, and a preparation method and application thereof. Background Art

[0002] Excessive reproduction of algae in water bodies will affect the ecological environment of water bodies. In the high temperature environment in summer, algae will grow exponentially. After the algae form water blooms, they cover the water surface and block the sunlight, causing aquatic plants and animals to die due to lack of oxygen, resulting in deterioration of water quality. Some algae, especially cyanobacteria, release toxic and harmful substances such as algae toxins and hydrogen sulfide when the cells are damaged or aged, which directly harm aquatic animals. The death and decay of algae will cause further deterioration of water quality and emit a foul odor. Therefore, algae outbreaks have become a major problem in the current river and lake water environment system, and the breeding of algae has also had a certain impact on human life and water safety.

[0003] At present, there are two main methods for removing algae: physical and chemical. The physical method is manual salvage or equipment treatment. The physical method will not pollute the environment, but it is time-consuming and labor-intensive, with high investment costs. It is suitable for smaller water bodies. The equipment treatment method also needs to consider issues such as later maintenance and depreciation of the equipment. The chemical method is to spread agents. One type is chemical agents, such as copper sulfate. Chemical agents remove algae quickly but are prone to secondary pollution and destroy the ecological balance of water bodies. The other type is biological agents, which use plant extracts to inhibit algae, but single plant active ingredients are easily degraded and the algae inhibition effect is limited. The preparation process of traditional algaecides is complicated and the production cost is high.

[0004] The spread of invasive plants has become a global ecological problem. They inhibit the growth of local species by competing for resources and releasing allelopathic substances, leading to ecological imbalance. In recent years, researchers have actively explored ways to utilize invasive plants as resources, using their extracts to inhibit algae in order to achieve the dual goals of ecological management and resource recycling. However, the use of a single plant extract has the problem of unstable inhibition effect and short duration. Summary of the invention

[0005] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide an anti-algae compound based on invasive plants and a preparation method and application thereof, so as to solve the problems in the prior art that the anti-algae effect of a single plant extract is not obvious and a single chemical agent is prone to secondary pollution.

[0006] To achieve the above-mentioned purpose and other related purposes, the present invention provides an anti-algae complex based on invasive plants, wherein the anti-algae complex comprises an extract of invasive plant powder and a chelating agent, wherein the invasive plant powder comprises powder of Spartina alterniflora, Bidens pilosa, Herba Lysimachiae and Glehnia littoralis, and the chelating agent comprises ferric citrate, disodium ethylenediaminetetraacetic acid (disodium EDTA) and phytic acid.

[0007] The present invention also provides a method for preparing the above-mentioned anti-algae compound based on invasive plants, comprising the following steps: S1. Preparation of invasive plant powder extracts: washing, drying I, and crushing Spartina alterniflora, Bidens pilosa, Herba Lysimachiae, and Gynura sylvestris, respectively, mixing them in proportion, and then performing alcohol extraction, filtering, and drying II to obtain invasive plant powder extracts; S2, complexation reaction: ferric citrate and disodium ethylenediaminetetraacetic acid are mixed in proportion and dissolved in warm water, and then phytic acid is added and mixed and dissolved to obtain a complexation solution; the invasive plant powder extract obtained in step S1 is added to the complexation solution, mixed and reacted, and the pH is controlled to be 5.5-6.0 to obtain an anti-algae complex.

[0008] Preferably, the algae-inhibiting compound is further granulated and coated, and the specific steps include: adding a binder to the algae-inhibiting compound for bonding and granulation, and spraying and coating with a chitosan solution to obtain a granular algae-inhibiting compound with a particle size of 1.0-2.0 mm.

[0009] The present invention also provides a use of the above-mentioned invasive plant-based algae-inhibiting compound in the removal of a single algae or in the removal of mixed algae in rivers / lakes / reservoirs / ponds / landscape pools.

[0010] As described above, the anti-algae compound based on invasive plants of the present invention and its preparation method and application have the following beneficial effects: The anti-algae compound based on invasive plants of the present invention uses invasive plants and chelating agents as raw materials, optimizes the ratio by using the characteristics of different raw materials, destroys the algae cell wall and blocks the algae photosynthesis system through the allelopathic substances extracted from plants, inhibits the reproduction of algae, adds chelating agents to chelate the trace elements required for algae growth contained in the water body, realizes nutrient blocking, enhances the slow-release cycle of the anti-algae compound through the synergistic effect of multiple components, and significantly improves the effect of inhibiting algae and removing algae, with a 7-day removal rate of >90%. Compared with the synergistic algae inhibition of mono-plants and chelating agents or the algae inhibition of chelating agent mixtures, the anti-algae and algae removal effect is improved by 1.5 to 2.5 times.

[0011] The present invention transforms invasive plants into high-value-added algae-inhibiting and algae-removing preparations, solves the problem of ecological management, realizes the efficient utilization of discarded plants, and reduces the cost of environmental management; it uses natural plants as the main raw material, does not cause secondary pollution, is safe and reliable to use, and provides new technology and experience for the ecological management of water bodies.

[0012] The coating method adopted in the preparation process of the present invention enhances the sustained-release period of the complex, is simple to operate, and has low cost, and is suitable for large-scale production. DETAILED DESCRIPTION

[0013] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0014] When a numerical range is disclosed herein, the above range is deemed to be continuous and includes the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise indicated, all ranges disclosed herein should be understood to include any and all sub-ranges included therein. For example, a specified range from "1 to 10" should be deemed to include any and all sub-ranges between a minimum of 1 and a maximum of 10. Exemplary sub-ranges of ranges 1 to 10 include, but are not limited to, 1 to 6.1, 3.5 to 7.8, 5.5 to 10, etc.

[0015] In addition, it should be understood that one or more method steps mentioned in the present invention do not exclude the existence of other method steps before or after the combination step or the insertion of other method steps between these explicitly mentioned steps, unless otherwise specified; and, unless otherwise specified, the numbering of each method step is only a convenient tool for identifying each method step, and is not intended to limit the order of arrangement of each method step or to define the scope of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the present invention without substantially changing the technical content.

[0016] A first aspect of the present invention provides an anti-algae complex based on invasive plants, the anti-algae complex comprising an extract of invasive plant powder and a chelating agent, the invasive plant powder comprising powders of Spartina alterniflora, Bidens pilosa, Herba Lysimachiae and Guayule, the chelating agent comprising ferric citrate, disodium ethylenediaminetetraacetic acid and phytic acid.

[0017] In the present invention, Spartina alterniflora can provide phenolic and terpenoid compounds, inhibit the algae antioxidant enzyme system, and block the algae photosynthetic system. Bidens pilosa can provide flavonoids and terpenoid compounds, inhibit the algae metabolic system, and interfere with algae nutrient absorption and material synthesis. Herba spatholobifolia can provide tropane alkaloids to destroy algae cell membranes. Guayule can provide guayulein to inhibit algae cell division and reproduction. The four plant extracts form a multi-component synergistic algae inhibition mechanism, which has a synergistic algae inhibition effect of blocking the photosynthesis system, inhibiting metabolism, cell membrane destruction, and inhibiting cell division.

[0018] In the present invention, the extracts of the invasive plant powder are all obtained by extracting the stems and leaves of the invasive plants.

[0019] The ferric citrate, disodium edetate and phytic acid are all commercially available.

[0020] In the anti-algae composite based on invasive plants of the present invention, the powder mass ratio of Spartina alterniflora, Bidens pilosa, Herba Lysimachiae and Gnayule is 33-37:18-22:13-17:8-12 based on dry basis mass. For example, 33~35:18~22:13~17:8~12, 35~37:18~22:13~17:8~12, 33~37:18~20:13~17:8~12, 33~37:20~22: 13~17:8~12, 33~37:18~22:13~15:8~12, 33~37:18~22:15~17:8~12, 33~37:18~22:13~17:8~10 or 33~37:18~22:13~17:10~12.

[0021] In the anti-algae complex based on invasive plants of the present invention, the mass ratio of ferric citrate, disodium ethylenediaminetetraacetic acid and phytic acid is 15-17:2-3:1-2, for example, 15-16:2-3:1-2, 16-17:2-3:1-2, 15-17:2-2.5:1-2, 15-17:2.5-3:1-2, 15-17:2-3:1-1.5 or 15-17:2-3:1.5-2.

[0022] In the present invention, ferric citrate can chelate phosphorus in the water, reduce the available phosphorus for algae, and affect its iron metabolic balance; disodium ethylenediaminetetraacetic acid can chelate metal ions such as iron, calcium, and magnesium, and block the absorption of trace elements by algae; phytic acid can wrap plant allelopathic substances, achieve slow release, and prolong the algae inhibition effect of allelopathic substances; phytic acid, as a natural synergist, can enhance Fe 3+ The synergistic effect improves the efficiency of algae inhibition.

[0023] In the anti-algae composite based on invasive plants of the present invention, the mass ratio of the invasive plant powder to the complexing agent is 3 to 5:1, for example, 3:1, 3.5:1, 4:1, 4.5:1 or 5:1.

[0024] The algae-inhibiting compound based on invasive plants of the present invention further comprises an adhesive, wherein the adhesive is 10-15 wt% of starch slurry. The starch slurry is conducive to the bonding and granulation of the powder of the algae-inhibiting compound.

[0025] The algae-inhibiting compound based on invasive plants of the present invention further comprises chitosan. The use of chitosan to make the coating is conducive to the storage of the algae-inhibiting compound.

[0026] The second aspect of the present invention provides a method for preparing the above-mentioned anti-algae compound based on invasive plants, comprising the following steps: S1. Preparation of invasive plant powder extracts: washing, drying I, and crushing Spartina alterniflora, Bidens pilosa, Herba Lysimachiae, and Gynura sylvestris, respectively, mixing them in proportion, and then performing alcohol extraction, filtering, and drying II to obtain invasive plant powder extracts; S2, complexation reaction: ferric citrate and disodium ethylenediaminetetraacetic acid are mixed in proportion and dissolved in warm water, and then phytic acid is added and mixed and dissolved to obtain a complexation solution; the extract of the invasive plant powder obtained in step S1 is added to the complexation solution, mixed and reacted, and the pH is controlled to be 5.5-6.0, 5.5-5.7, 5.7-5.9 or 5.9-6.0, etc., to obtain an anti-algae complex.

[0027] In the preparation method of the present invention, the algae-inhibiting complex is further granulated and coated, and the specific steps include: adding an adhesive to the algae-inhibiting complex for bonding and granulation, and spraying and coating with a chitosan solution to obtain a granular algae-inhibiting complex with a particle size of 1.0-2.0 mm, 1.0-1.2 mm, 1.2-1.4 mm, 1.4-1.6 mm, 1.6-1.8 mm or 1.8-2.0 mm.

[0028] The concentration of the chitosan solution is 1-3 wt %, for example 1-2 wt % or 2-3 wt %. The amount of chitosan used is 2-5 % of the mass of the granules obtained by granulation, for example 2-3 %, 3-4 % or 4-5 %.

[0029] The adhesive is 10 wt% to 15 wt% of starch slurry, for example, 10 wt%, 11 wt%, 12 wt%, 13 wt%, 14 wt% or 15 wt%.

[0030] The amount of the adhesive added is 10% to 15% of the mass of the algae-inhibiting compound, for example, 10% to 12%, 12% to 14% or 14% to 15%.

[0031] The granulation and coating can be completed in a fluidized bed granulator, and the granulation and coating are integrated into a process, which can shorten the production cycle.

[0032] In the preparation method of the present invention, the temperature of the drying I in step S1 is 50°C to 60°C, for example, 50°C to 55°C or 55°C to 60°C.

[0033] The drying I in step S1 is drying to a moisture content of ≤8%.

[0034] The pulverization in step S1 is pulverization to a particle size of ≤180 μm.

[0035] The solvent used in the alcohol extraction in step S1 is a 50% to 70% ethanol solution.

[0036] The material-liquid ratio in the alcohol extraction process in step S1 is 1:2-5. For example, 1:2-3, 1:3-4 or 1:4-5.

[0037] The alcohol extraction in step S1 is microwave-assisted extraction, the power of the microwave is 450W-500W, 450W-460W, 460W-470W, 470W-480W, 480W-490W or 490W-500W, and the extraction time is 10-60min. For example, 10-20min, 20-30min, 30-40min, 40-50min or 50-60min.

[0038] The drying II in step S1 is spray drying.

[0039] In the preparation method of the present invention, the ratio of the total amount of ferric citrate and disodium edetate to water in step S2 is 1 to 2: 1. For example, 1 to 1.2: 1, 1.2 to 1.4: 1, 1.4 to 1.6: 1, 1.6 to 1.8: 1 or 1.8 to 2: 1.

[0040] The temperature of the warm water in step S2 is 40°C to 50°C, for example, 40°C to 42°C, 42°C to 44°C, 44°C to 46°C, 46°C to 48°C or 48°C to 50°C.

[0041] The mixing reaction time in step S2 is 30 min to 60 min, for example, 30 min to 35 min, 35 min to 40 min, 40 min to 45 min, 45 min to 50 min, 50 min to 55 min or 55 min to 60 min.

[0042] The temperature of the mixing reaction in step S2 is 40°C to 50°C, for example, 40°C to 42°C, 42°C to 44°C, 44°C to 46°C, 46°C to 48°C or 48°C to 50°C.

[0043] In the preparation method of the present invention, the particle size of the granular algae-inhibiting composite in step S3 is 1.0-2.0 mm, for example, 1.0-1.2 mm, 1.2-1.4 mm, 1.4-1.6 mm, 1.6-1.8 mm or 1.8-2.0 mm.

[0044] The third aspect of the present invention provides an application of the above-mentioned anti-algae compound based on invasive plants in the removal of a single algae or in the removal of mixed algae in a river / lake / reservoir / pond / landscape pool. The algae may be cyanobacteria, green algae, dinoflagellates or euglena, etc. The cyanobacteria may be Microcystis alum. The green algae may be Chlorella vulgaris. The dinoflagellates may be Ceratium hornulatum. The euglena may be Euglena rubrum.

[0045] In the present invention, the experimental method for algae removal is as follows: a certain amount of algae is placed in a culture vessel or water from a natural water body rich in algae is taken, different amounts of the compound are added to the water body containing the algae, and stirred evenly to make the concentration of the algae-inhibiting compound in the water body 100-450 mg / L, and cultured for 1-7 days under the conditions of a temperature of 25-30°C and sufficient light, and the removal rate is calculated by counting the number of surviving algae in the culture dish. In practical applications, different concentrations of the compound can be selected according to actual needs.

[0046] Algae removal rate determination method: The initial density of algae is denoted as X 1 Weigh different amounts of the three complexes and add them to the culture dish containing algae, stir evenly, and then culture them at 25-30°C and sufficient light for 1-7 days. Count the number of surviving algae and record them as X. 2 . Algae removal rate of anti-algae compound = (X 1 -X 2 ) / X 1 × 100%. The counting method is not particularly limited, and the present invention adopts the microscope cell counting method.

[0047] Example 1 Preparation of an anti-algae compound based on invasive plants: S1. Wash the stems and leaves of Spartina alterniflora, Bidens pilosa, Herba Lysimachiae, and Gnaphalium separately, dry them with hot air at 60°C to a moisture content of ≤8%, crush them to a particle size of ≤180 μm, mix them in a mass ratio of 33-37:18-22:13-17:8-12, add 50% ethanol solution with a material-liquid mass ratio of 1:5, extract them at a microwave power of 500 W for 10 min, filter and spray-dry, and obtain an extract of invasive plant powder.

[0048] S2. Dissolve ferric citrate and disodium ethylenediaminetetraacetate in 40° C. warm water, and add phytic acid (ferric citrate, disodium ethylenediaminetetraacetate, phytic acid in a mass ratio of 15-17:2-3:1-2), with a solid-liquid ratio of 1:1, and stir until completely dissolved to obtain a complexing solution; add the extract powder obtained in step S1 to the complexing solution, stir and react at 40° C. for 30 minutes, and control the pH value to 5.5-6.0 to obtain a mixed material.

[0049] S3. Add the mixed material directly into the fluidized bed granulator, first spray 10wt% starch slurry to form granules (corn starch: water = 1:9, the addition amount is 10% of the mass of the mixed material), then add 2wt% chitosan solution (the amount of chitosan added is 3% of the mass of the granules obtained by granulation), control the inlet air temperature at 50℃ and the outlet air temperature at 40℃ to obtain coated granules with a particle size of 1.0~2.0mm in the form of an anti-algae complex.

[0050] Example 2 1) Experimental water: cyanobacteria solution (Microcystis aeruginosa), algae density in the solution 1×10 6 Pieces / ml.

[0051] 2) Culture conditions: light intensity 2500 lux, temperature 26°C.

[0052] 3) Experimental materials: Experimental group: a multi-component complex was formed by using four-component plants and a ternary complexing agent, the components by mass were: 33 parts of Spartina alterniflora, 18 parts of Bidens pilosa, 17 parts of Herba Glehniae, 12 parts of Guayule, 17 parts of ferric citrate, 2 parts of disodium EDTA, and 1 part of phytic acid. The algae-inhibiting complex particles were prepared according to the preparation method of Example 1.

[0053] Control group: a monovalent plant and a ternary complexing agent are used to form a complex, the plant is airplane grass, the complexing agent is ferric citrate, disodium EDTA, and phytic acid, and the weight ratio is: 80 parts of airplane grass, 17 parts of ferric citrate, 2 parts of disodium EDTA, and 1 part of phytic acid. The airplane grass is washed, dried, crushed, and alcohol-extracted according to the preparation method of Example 1, and the complexing agent is used to prepare a complexing liquid according to Example 1, and then the algae-inhibiting complex particles are prepared.

[0054] 4) Experimental methods: Take 6L of the solution with a concentration of 1×10 6 The cyanobacteria solution of 100 mg / ml was placed in 6 1L culture vessels of the same capacity, and the culture vessels were numbered from 21 to 26#; 100 mg, 200 mg, and 300 mg of the algae-inhibiting compound particles of the four-element plant were weighed, and added to the culture vessels containing cyanobacteria from 21 to 23# in order; 100 mg, 200 mg, and 300 mg of the algae-inhibiting compound particles of the one-element plant were weighed, and added to the culture vessels containing cyanobacteria from 24 to 26# in order, and stirred evenly; then cultured at 26°C and sufficient light for 7 days, and the number of cyanobacteria was recorded. After the culture was completed, the removal rate was calculated.

[0055] 5) Experimental results The concentrations of the compound in the experimental group 21~23# were 100mg / L, 200mg / L, and 300mg / L, respectively. After 7 days of cultivation, the algae removal rates were 67.2%, 83.6%, and 95.2%, respectively. The concentrations of the compound in the control group 24~26# were 100mg / L, 200mg / L, and 300mg / L, respectively. After 7 days of cultivation, the algae removal rates were 33.5%, 45.6%, and 53.3%, respectively. See Table 1 for details.

[0056] Table 1

[0057] Combined with the data in Table 1, it can be seen that the removal rate of the experimental group complex increased rapidly on the third day after addition, and maintained a high removal rate on the seventh day. The concentration reached 300 mg / L, and the removal rate could reach more than 95%. The removal rate of the control group complex increased more slowly, with the highest removal rate of 53.3% at 7 days. The removal rate of the experimental group complex at 7 days was 1.8 to 2 times that of the control group complex, indicating that the multi-component complex of plants and chelating agents can play a synergistic algae inhibition role and can take effect quickly. The synergistic effect of multiple components also allows the algae inhibition substances to be slowly released and continuously exert the algae inhibition effect. The control group is a complex formed by a single plant and a chelating agent, and the synergistic effect is not obvious. Compared with the synergistic algae inhibition of the four-component plant and the one-component plant and the ternary chelating agent, the algae inhibition rate increased by 78.6%.

[0058] Example 3 1) Experimental water: Green algae solution (Chlorella vulgaris), algae density in the solution 1×10 6 Pieces / ml.

[0059] 2) Culture conditions: light intensity 3000 lux, temperature 30℃.

[0060] 3) Experimental materials: Experimental group: a multi-component complex was formed by using four-component plants and a three-component complexing agent, the components by mass were: 35 parts of Spartina alterniflora, 20 parts of Bidens pilosa, 15 parts of Herba Glehniae, 10 parts of Guayule, 16 parts of ferric citrate, 2.5 parts of disodium EDTA, and 1.5 parts of phytic acid. The algae-inhibiting complex particles were prepared according to the preparation method of Example 1.

[0061] Control group: mainly composed of a ternary complexing agent mixture without adding plants, which is calculated by mass as follows: 80 parts of ferric citrate, 12.5 parts of disodium EDTA, and 7.5 parts of phytic acid to form a complexing agent mixture.

[0062] 4) Experimental methods: Take 6L of the solution with a concentration of 1×10 6The green algae solution of 100 mg / ml was placed in 6 1L culture vessels of the same capacity, and the culture vessels were numbered from 31 to 36#; 150 mg, 250 mg, and 350 mg of the four-element plant composite particles were weighed respectively, and added to the culture vessels containing green algae in 31 to 33# in sequence; 150 mg, 250 mg, and 350 mg of the complexing agent mixture were weighed according to the proportion of the complexing agent, and added to the culture vessels containing green algae in 34 to 36# in sequence, and stirred evenly; then cultured at 30°C and sufficient light for 7 days, and the number of green algae was recorded. After the culture was completed, the removal rate was calculated.

[0063] 5) Experimental results: The concentrations of the 31~33# multi-component complexes were 150mg / L, 250mg / L, and 350mg / L, respectively. After 7 days of cultivation, the green algae removal rates were 72.9%, 91.3%, and 96.3%, respectively. The concentrations of the 34~36# complexing agent mixtures were 150mg / L, 250mg / L, and 350mg / L, respectively. After 7 days of cultivation, the algae removal rates were 33.7%, 39.3%, and 44.2%, respectively. See Table 2 for details.

[0064] Table 2

[0065] Combined with the data in Table 2, it can be seen that the removal rate of the anti-algae compound in the experimental group increased rapidly on the third day after addition, and maintained a high removal rate on the seventh day. When the concentration reached 250 mg / L or more, the 7d removal rate reached more than 90%, and when the concentration reached 350 mg / L, the removal rate could reach 96.3%. The removal rate of the complexing agent mixture in the control group reached a maximum of 69.3% on the third day, and the removal rate began to decline continuously on the fifth day. The 7d removal rate of the multi-component complex was twice that of the complexing agent mixture. This shows that the complexing agent mixture can inhibit algae by complexing the nutrients such as phosphorus and iron required for algae growth, and the algae removal is effective quickly, but due to the lack of synergistic anti-algae effects such as plant inhibition of photosynthetic system, the duration of algae inhibition is short and it is difficult to play a long-term role. In addition, the dosage of the complexing agent mixture alone is large, which is easy to cause secondary pollution.

[0066] Example 4 1) Experimental water: dinoflagellate solution (horn algae), algae density in the solution is 1×10 6 Pieces / ml.

[0067] 2) Culture conditions: light intensity 2000 lux, temperature 25℃.

[0068] 3) Experimental materials: Experimental group: a multi-component complex was formed by using four-component plants and a ternary complexing agent, the components by mass were: 37 parts of Spartina alterniflora, 22 parts of Bidens pilosa, 13 parts of Herba Glehniae, 8 parts of Guayule, 15 parts of ferric citrate, 3 parts of disodium EDTA, and 2 parts of phytic acid. The algae-inhibiting complex particles were prepared according to the preparation method of Example 1.

[0069] Control group: a compound was formed by using three plants and a three-component chelating agent, the components by mass being: 35 parts of Bidens pilosa, 25 parts of Herba schoenopraeci, 20 parts of Guayule, 15 parts of ferric citrate, 3 parts of disodium EDTA, and 2 parts of phytic acid. The algae-inhibiting compound particles were prepared according to the preparation method of Example 1.

[0070] 4) Experimental methods: Take 6L of the solution with a concentration of 1×10 6 The dinoflagellate solution with 1000 mg / ml was placed in 6 1L culture vessels of the same capacity, and the culture vessels were numbered from 41 to 46#; 200 mg, 300 mg, and 400 mg of multi-component composite particles were weighed respectively, and added to the culture vessels containing dinoflagellates from 41 to 43# in sequence; the control group weighed 200 mg, 300 mg, and 400 mg of the composite in proportion, and added to the culture vessels containing dinoflagellates from 44 to 46# in sequence, and stirred evenly; then cultured at 25°C and sufficient light for 7 days, and the number of dinoflagellates was recorded. After the culture was completed, the removal rate was calculated.

[0071] 5) Experimental results: The concentrations of 41~43# anti-algae compounds were 200mg / L, 300mg / L, and 400mg / L, respectively. After 7 days of cultivation, the removal rates of dinoflagellates were 85.3%, 94.8%, and 99.5%, respectively. The concentrations of 44~46# control group compounds were 200mg / L, 300mg / L, and 400mg / L, respectively. After 7 days of cultivation, the removal rates of dinoflagellates were 52.6%, 69%, and 79.4%, respectively. See Table 3 for details.

[0072] Table 3

[0073] Combined with the data in Table 3, it can be seen that the removal rate of the multi-component complex in the experimental group increased rapidly on the first day after addition, and remained at a high removal rate on the 7th day. When the concentration reached 300 mg / L or more, the 5-day removal rate reached more than 90%. When the concentration reached 400 mg / L, the 7-day removal rate reached 99.5%. The removal rate of the control group complex also increased steadily after addition, but due to the lack of inhibition of algae photosynthesis by Spartina alterniflora, the 7-day removal rate of the control group complex reached a maximum of 79.4%. Compared with the synergistic effect of the four-element plant and the three-element plant and the three-element complexing agent, the algae inhibition rate increased by 25%.

[0074] Example 5 1) Experimental water: taken from a natural river. The algae species in the water are mainly mixed algae such as cyanobacteria, green algae and diatoms. The algae density is 5×10 5 Pieces / ml.

[0075] 2) Culture conditions: natural light, temperature 25℃.

[0076] 3) Experimental materials: Experimental group: a multi-component complex was formed by using four-component plants and a ternary complexing agent, the components by mass were: 33 parts of Spartina alterniflora, 18 parts of Bidens pilosa, 17 parts of Herba Glehniae, 12 parts of Guayule, 17 parts of ferric citrate, 2 parts of disodium EDTA, and 1 part of phytic acid. The algae-inhibiting complex particles were prepared according to the preparation method of Example 1.

[0077] Control group: a compound was formed by using a binary plant and a ternary complexing agent, the components by mass being: 45 parts of airplane grass, 35 parts of guayule, 17 parts of ferric citrate, 2 parts of disodium EDTA, and 1 part of phytic acid. The algae-inhibiting compound particles were prepared according to the preparation method of Example 1.

[0078] 4) Experimental methods: Take 6L of natural river water, the concentration of mixed algae in the water is 5×10 5 / ml, and placed in 6 1L culture vessels of the same capacity on average, and the culture vessels were numbered from 51 to 56#; the experimental group weighed 200mg, 300mg, and 400mg of multi-component composite particles according to the proportion, and added them to the culture vessels 51 to 53# containing natural river water in sequence, and the control group weighed 200mg, 300mg, and 400mg of the composite according to the proportion, and added them to the culture vessels 54 to 56# containing natural river water in sequence, and stirred evenly; then cultured at 25℃ and sufficient natural light for 7 days, and recorded the number of algae. After the culture was completed, the removal rate was calculated.

[0079] 5) Experimental results: The concentrations of the 51~53# multi-component complexes were 200mg / L, 300mg / L, and 400mg / L, respectively. After 7 days of cultivation, the algae removal rates were 83.5%, 92.2%, and 97.8%, respectively. The concentrations of the 54~56# control group complexes were 200mg / L, 300mg / L, and 400mg / L, respectively. After 7 days of cultivation, the algae removal rates were 47.6%, 57.6%, and 68.2%, respectively. See Table 4 for details.

[0080] Table 4

[0081] Combined with the data in Table 4, it can be seen that the removal rate of the multi-component complex in the experimental group increased rapidly on the third day after addition, and remained at a high removal rate on the seventh day. When the concentration reached 300 mg / L or more, the 7-day removal rate reached more than 90%. When the concentration reached 400 mg / L, the 7-day removal rate reached 97.8%. The removal rate of the control group complex increased slowly, and when the concentration reached 400 mg / L, the 7-day removal rate reached 68.2%. The plants in the control group lacked Spartina alterniflora and Bidens pilosa, lacked inhibition of algae photosynthesis and cell metabolism, and the algae removal was slow. Compared with the synergistic effect of the four-element plant and the two-element plant and the ternary complexing agent, the algae inhibition rate increased by 43%.

[0082] Example 6 1) Experimental water: The water was taken from a natural lake. The algae species in the water were mainly cyanobacteria, green algae, and dinoflagellates. The algae density was 1×10 6 Pieces / ml.

[0083] 2) Culture conditions: natural light, temperature 28℃.

[0084] 3) Experimental materials: Experimental group: a multi-component complex was formed by using four-component plants and a three-component complexing agent, the components by mass were: 35 parts of Spartina alterniflora, 20 parts of Bidens pilosa, 15 parts of Herba Glehniae, 10 parts of Guayule, 16 parts of ferric citrate, 2.5 parts of disodium EDTA, and 1.5 parts of phytic acid. The algae-inhibiting complex particles were prepared according to the preparation method of Example 1.

[0085] Control group: A four-component plant complex was formed, the components by mass were: 40 parts of Spartina alterniflora, 25 parts of Bidens pilosa, 20 parts of Herba Lycopodii, and 15 parts of Gnayule, which were cleaned, dried, crushed, and alcohol-extracted according to the preparation method of Example 1, and corn starch slurry and chitosan were added to form granules.

[0086] 4) Experimental methods: Take 6L of natural lake water, the concentration of mixed algae in the water is 1×10 6 / ml, and placed in 6 1L culture vessels of the same capacity on average, and the culture vessels were numbered from 61 to 66#; the experimental group weighed 250mg, 350mg, and 450mg of multi-component composite particles according to the proportion, and added them to the culture vessels 61 to 63# containing natural lake water in sequence, and the control group weighed 250mg, 350mg, and 450mg of the composite according to the proportion, and added them to the culture vessels 64 to 66# containing natural lake water in sequence, and stirred evenly; then cultured at 28℃ and sufficient natural light for 7 days, and recorded the number of algae. After the culture was completed, the removal rate was calculated.

[0087] 5) Experimental results: The concentrations of the 61~63# multi-component complexes were 250mg / L, 350mg / L, and 450mg / L, respectively. After 7 days of cultivation, the algae removal rates were 89.1%, 95.8%, and 98.6%, respectively. The concentrations of the 64~66# complexes were 250mg / L, 350mg / L, and 450mg / L, respectively. After 7 days of cultivation, the algae removal rates were 51.6%, 60.5%, and 64.8%, respectively. See Table 5 for details.

[0088] Table 5

[0089] Combined with the data in Table 5, it can be seen that the removal rate of the multi-component complex in the experimental group increased rapidly on the third day after addition, and maintained a high removal rate on the seventh day. When the concentration reached 350 mg / L or more, the 7-day removal rate reached more than 95%. When the concentration reached 450 mg / L, the 7-day removal rate reached 98.6%. The removal rate of the control group complex increased rapidly on the fifth day after addition, and the concentration reached 450 mg / L, and the 5-day removal rate reached 76.1%, but the removal rate decreased again on the seventh day, falling below 65%. The control group was only a four-component plant complex, lacking the synergistic effect of the ternary complexing agent on algae nutrition blocking and the slow release effect of phytic acid on plant allelopathic substances, so the algae inhibition rate decreased.

[0090] Example 7 1) Experimental water: taken from a natural reservoir. The algae species in the water are mainly cyanobacteria, green algae, dinoflagellates, and mixed algae. The algae density is 2×10 6 Pieces / ml.

[0091] 2) Culture conditions: natural light, temperature 26℃.

[0092] 3) Experimental materials: Experimental group: a multi-component complex was formed by using four-component plants and a ternary complexing agent, the components by mass were: 37 parts of Spartina alterniflora, 22 parts of Bidens pilosa, 13 parts of Herba Glehniae, 8 parts of Guayule, 15 parts of ferric citrate, 3 parts of disodium EDTA, and 2 parts of phytic acid. The algae-inhibiting complex particles were prepared according to the preparation method of Example 1.

[0093] Control group: a multi-component complex was formed by using ternary plants and ternary complexing agents, the components by mass being: 45 parts of Spartina alterniflora, 20 parts of Herba Glechomae, 15 parts of Guayule, 15 parts of ferric citrate, 3 parts of disodium EDTA, and 2 parts of phytic acid. The algae-inhibiting complex particles were prepared according to the preparation method of Example 1.

[0094] 4) Experimental methods: Take 6L of natural reservoir water, the concentration of mixed algae in the water is 2×10 6 / ml, and placed in 6 1L culture vessels of the same capacity on average, and the culture vessels were numbered from 71 to 76#; the experimental group weighed 250mg, 350mg, and 450mg of multi-component composite particles in proportion, and added them to the culture vessels 71 to 73# containing natural reservoir water in sequence, and the control group weighed 250mg, 350mg, and 450mg of the composite in proportion, and added them to the culture vessels 74 to 76# containing natural reservoir water in sequence, and stirred evenly; then cultured at 26℃ and sufficient natural light for 7 days, and recorded the number of algae. After the culture was completed, the removal rate was calculated.

[0095] 5) Experimental results: The concentrations of 71~73# anti-algae compounds were 250mg / L, 350mg / L, and 450mg / L, respectively. After 7 days of cultivation, the algae removal rates were 90.3%, 96.6%, and 99.2%, respectively. The concentrations of 74~76# compounds were 250mg / L, 350mg / L, and 450mg / L, respectively. After 7 days of cultivation, the algae removal rates were 62.6%, 73.4%, and 82.2%, respectively. See Table 6 for details.

[0096] Table 6

[0097] Combined with the data in Table 6, it can be seen that the removal rate of the experimental group multi-component complex began to increase rapidly on the first day after addition, and maintained a high removal rate on the 7th day. When the concentration reached 250 mg / L or more, the 7d removal rate could reach more than 90%. When the concentration reached 450 mg / L, the 7d removal rate reached 99.2%. The removal rate of the control group complex began to increase on the 3rd day after addition, and the concentration reached 450 mg / L, and the 7d removal rate reached 82.2%. The control group complex lacked the inhibitory effect of Bidens pilosa on algae metabolism, and the algae inhibition rate was difficult to increase to 85%. Compared with the synergistic effect of the four-element plant and the three-element plant and the three-element complexing agent, the algae inhibition rate increased by 20.6%.

[0098] In summary, the present invention provides an anti-algae compound based on invasive plants, its preparation and application, converts invasive plants into high-value-added anti-algae preparations, and through the synergistic effect of plant active ingredients and chelating agents, increases the slow-release period of anti-algae factors, while improving the anti-algae effect, and can be applied to eutrophic rivers, lakes and other water bodies to inhibit algae outbreaks and purify water quality. In addition, the anti-algae compound based on invasive plants of the present invention uses natural raw materials and slow-release technology, reduces environmental risks, develops a simple, low-cost preparation process, and is suitable for large-scale production.

[0099] Therefore, the present invention effectively overcomes various shortcomings in the prior art and has high industrial utilization value.

[0100] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. An anti-algae compound based on invasive plants, characterized in that: The anti-algae compound comprises an extract of invasive plant powder and a chelating agent, wherein the invasive plant powder comprises powder of Spartina alterniflora, Bidens pilosa, Herba Lysimachiae and Guayule, and the chelating agent comprises ferric citrate, disodium ethylenediaminetetraacetic acid and phytic acid.

2. The anti-algae compound based on invasive plants according to claim 1, characterized in that: Calculated on a dry basis, the powder mass ratio of the Spartina alterniflora, Bidens pilosa, Herba Lysimachiae, and Gnayule is 33-37:18-22:13-17:8-12.

3. The anti-algae compound based on invasive plants according to claim 1, characterized in that: The mass ratio of the ferric citrate, disodium edetate and phytic acid is 15-17:2-3:1-2.

4. The anti-algae compound based on invasive plants according to claim 1, characterized in that: The mass ratio of the invasive plant powder to the complexing agent is 3-5:

1.

5. The anti-algae compound based on invasive plants according to claim 1, characterized in that: The algae-inhibiting compound further comprises an adhesive, wherein the adhesive is 10-15 wt% of starch slurry; And / or, the anti-algae compound further comprises chitosan.

6. A method for preparing an anti-algae compound based on invasive plants according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Preparation of invasive plant powder extracts: washing, drying I, and crushing Spartina alterniflora, Bidens pilosa, Herba Lysimachiae, and Gynura sylvestris, respectively, mixing them in proportion, and then performing alcohol extraction, filtering, and drying II to obtain invasive plant powder extracts; S2, complexation reaction: ferric citrate and disodium ethylenediaminetetraacetic acid are mixed in proportion and dissolved in warm water, and then phytic acid is added and mixed and dissolved to obtain a complexation liquid; the extract of the invasive plant powder obtained in step S1 is added to the complexation liquid, mixed and reacted, and the pH is controlled to be 5.5-6.0 to obtain an anti-algae complex.

7. The method for preparing the anti-algae compound based on invasive plants according to claim 6, characterized in that: The algae-inhibiting compound is further granulated and coated, and the specific steps include: adding a binder to the algae-inhibiting compound for bonding and granulation, and spraying and coating with a chitosan solution to obtain a granular algae-inhibiting compound with a particle size of 1.0-2.0 mm.

8. The method for preparing the anti-algae compound based on invasive plants according to claim 6, characterized in that: The drying temperature in step S1 is 50°C to 60°C; And / or, the drying I in step S1 is drying to a moisture content of ≤8%; And / or, the pulverization in step S1 is pulverization to a particle size of ≤180 μm; And / or, the solvent used in the alcohol extraction in step S1 is a 50% to 70% ethanol solution; And / or, the mass ratio of material to liquid during the alcohol extraction process in step S1 is 1:2-5; And / or, the alcohol extraction in step S1 is performed by microwave-assisted extraction, the microwave power is 450W-500W, and the extraction time is 10-60min; And / or, the drying II in step S1 is spray drying.

9. The method for preparing the anti-algae compound based on invasive plants according to claim 6, characterized in that: The mass ratio of the total amount of ferric citrate, disodium edetate, and phytic acid to water in step S2 is 1-2:1; And / or, the temperature of the warm water in step S2 is 40°C to 50°C; And / or, the mixing reaction time in step S2 is 30 min to 60 min; And / or, the temperature of the mixing reaction in step S2 is 40°C to 50°C.

10. Use of the invasive plant-based algae-inhibiting compound according to any one of claims 1 to 5 in the removal of a single algae or in the removal of mixed algae in rivers / lakes / reservoirs / ponds / landscape pools.

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