Mouse respiration monitoring and adjusting method, mouse respiration monitoring and adjusting system and medium
By obtaining the mouse respiratory monitoring signal and responding, using pressure sensor data to determine the respiratory status, transmitting target light waves to inhibit or excite neurons, the problems of poor respiratory monitoring and cumbersome monitoring in the prior art are solved, and efficient respiratory monitoring and adjustment are achieved.
Patent Information
- Application Number
- CN202311580504.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-23
- Publication Date
- 2025-05-23
AI Technical Summary
The prior art has poor effect on respiration monitoring in mice and is complicated to monitor.
By acquiring the monitoring signal, the pressure data of the pressure sensor is acquired in response to the signal, determining whether the respiratory state of the mice is abnormal, and the target light wave is transmitted to neurons carrying the photosensitive gene for inhibition or excitation.
Efficient mouse respiratory monitoring and adjustment were achieved, helping experimenters to understand the relationship between respiratory and neuronal activity more comprehensively.
Smart Images
Figure CN120021972A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of mouse respiration monitoring and adjustment, and in particular to a mouse respiration monitoring and adjustment method, device, mouse respiration monitoring and adjustment system and medium. Background Art
[0002] Mouse respiratory testing is of key significance in biomedical research. By monitoring respiratory activity, we can gain a deep understanding of the physiological state of mice, including metabolic levels, the effects of drugs on the respiratory system, and the degree of damage to the respiratory system by toxic substances. In addition, many diseases, such as respiratory diseases and nervous system diseases, can lead to abnormal breathing.
[0003] However, current respiratory monitoring of mice is ineffective and cumbersome. Summary of the invention
[0004] Based on this, it is necessary to propose a mouse respiratory monitoring adjustment method, a mouse respiratory monitoring adjustment system and a medium to address the technical problems of poor effect and cumbersome monitoring of mouse respiratory monitoring in the prior art.
[0005] In a first aspect, a method for monitoring and adjusting mouse respiration is provided, the method comprising:
[0006] Obtain monitoring signals;
[0007] In response to the monitoring signal, obtaining pressure data of the pressure sensor;
[0008] determining, based on the pressure data, whether the respiratory state of the target mouse in the whole body plethysmography device is abnormal;
[0009] If it is abnormal, the target light waves corresponding to the breathing state will be transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons.
[0010] In a second aspect, a mouse respiratory monitoring and adjustment system is provided, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor implements the steps of the above-mentioned mouse respiratory monitoring and adjustment method when executing the computer program.
[0011] In a third aspect, a computer-readable storage medium is provided, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the steps of the above-mentioned mouse respiratory monitoring and adjustment method are implemented.
[0012] The mouse respiration monitoring and adjustment method proposed in the present invention obtains the monitoring signal and responds to the monitoring signal to obtain the pressure data of the pressure sensor, and then determines whether the respiration state of the target mouse in the whole body plethysmography device is abnormal based on the pressure data. Finally, if it is abnormal, the target light wave corresponding to the respiration state is transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons. The mouse respiration can be efficiently monitored and adjusted, thereby facilitating the experimenter to have a more comprehensive understanding of the relationship between respiration and neuronal activity. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0014] in:
[0015] Figure 1 A diagram showing an application environment of a method for adjusting mouse respiration monitoring in one embodiment;
[0016] Figure 2 A flowchart of a method for adjusting mouse respiration monitoring in one embodiment;
[0017] Figure 3 It is a structural block diagram of a mouse respiration monitoring and adjustment system in one embodiment. DETAILED DESCRIPTION
[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of the present application; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit the present application; the terms "including" and "having" and any variations thereof in the specification and claims of the present application and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification and claims of the present application or the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.
[0019] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.
[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0021] The mouse respiration monitoring and adjustment method provided in the embodiment of the present invention can be applied in the following aspects: Figure 1 In the mouse breathing monitoring and adjustment system, the mouse breathing monitoring and adjustment system comprises: a whole body plethysmography device 201, a wireless optogenetic device 205, a feedback device (not shown) and an integrated microprocessor device 204, the whole body plethysmography device 201 is provided with a pressure sensor 202, and the whole body plethysmography device 201, the wireless optogenetic device 205, and the feedback device are electrically connected to the integrated microprocessor device 204 respectively;
[0022] Further, in a preferred implementation, the whole body plethysmography device is used to obtain pressure data from the pressure sensor and send the pressure data to the integrated microprocessor device;
[0023] Step A: the integrated microprocessor device converts the pressure data into breathing parameters and sends the breathing parameters to the feedback device;
[0024] Among them, respiratory parameters include tidal volume, peak expiratory flow rate, respiratory rate, etc.
[0025] Specifically, the integrated microprocessor device includes a closed body scanning box, in which the mouse is placed, and the volume change of the gas in the box can be sensed by a pressure sensor, converted into a breathing curve through an algorithm, and the breathing parameters are calculated through the breathing curve.
[0026] Step B: the feedback device is used to generate an adjustment instruction according to the breathing parameter, and send the adjustment instruction to the integrated microprocessor device;
[0027] The feedback device may be a user terminal device, such as a smart phone, etc. The integrated microprocessor device 204 may be, but is not limited to, various personal computers, laptops, smart phones, tablet computers, and portable wearable devices.
[0028] Specifically, the feedback device accepts breathing parameters and displays the breathing parameters in the user interface of the feedback device. The user can issue adjustment instructions based on the breathing parameters. Of course, the user can also compare the breathing parameters of mice in a normal state with the breathing parameters to obtain difference data, evaluate the difference data, and obtain a difference level. When the difference level is greater than a preset first level, an adjustment instruction is generated, and when the difference level is less than a preset second level, an adjustment instruction is generated. Among them, a difference level greater than the preset first level means that the breathing parameters are too large, and a difference level less than the preset second level means that the breathing parameters are too small.
[0029] Step C: the integrated microprocessor device is used to generate an adjustment data packet according to the adjustment instruction, and send the adjustment data packet to the wireless optogenetic device, wherein the adjustment data includes target light wave data;
[0030] The adjustment instructions may include inhibition instructions and excitement instructions.
[0031] Specifically, an adjustment data packet corresponding to the inhibition instruction is sent to the wireless optogenetics device, or an adjustment data packet corresponding to the excitation instruction is sent to the wireless optogenetics device.
[0032] Step D: The wireless optogenetic device is used to transmit the target light wave corresponding to the target light wave data to the neurons carrying the light-sensitive gene of the target mouse according to the target light wave data in the adjustment data packet, so as to inhibit or excite the neurons.
[0033] The adjustment data includes target light wave data for inhibiting neurons carrying light-sensitive genes of target mice, or target light wave data for exciting neurons carrying light-sensitive genes of target mice.
[0034] Furthermore, in a preferred implementation, the whole body plethysmography device includes: a closed body scanning box, and the closed body scanning box is provided with the pressure sensor.
[0035] Furthermore, in a preferred implementation, the pressure sensor is connected to a pressure conversion module, the pressure conversion module includes a pressure transducer and an amplifier, and the pressure conversion module is connected to the integrated microprocessor device.
[0036] Furthermore, in a preferred implementation, the wireless optogenetics device is disposed on the head of the target mouse.
[0037] See also Figure 2 As shown, Figure 2 A schematic diagram of a flow chart of a method for monitoring and adjusting mouse respiration provided in one embodiment of the present invention.
[0038] The mouse respiratory monitoring and adjustment method comprises the following steps:
[0039] Step S101: Acquire monitoring signals;
[0040] Step S102: Responding to the monitoring signal, obtaining pressure data of the pressure sensor;
[0041] Step S103: determining whether the respiratory state of the target mouse in the whole body plethysmography device is abnormal according to the pressure data;
[0042] In one embodiment, the pressure data is processed to obtain a breathing curve, and the current breathing parameters are calculated based on the breathing curve; the current breathing parameters are compared with preset normal mouse breathing parameters to determine whether the breathing state is abnormal.
[0043] Step S104: If abnormal, the target light wave corresponding to the breathing state is transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons.
[0044] In this embodiment, the respiratory state can be a state with lower respiratory parameters and a state with higher respiratory parameters. When the respiratory state is a state with lower respiratory parameters, a target light wave for exciting neurons carrying light-sensitive genes of the target mouse is used to excite the neurons; when the respiratory state is a state with higher respiratory parameters, a target light wave for inhibiting neurons carrying light-sensitive genes of the target mouse is used to excite the neurons.
[0045] The mouse respiration monitoring and adjustment method of the present embodiment obtains the monitoring signal and responds to the monitoring signal to obtain the pressure data of the pressure sensor, and then determines whether the respiration state of the target mouse in the whole body plethysmography device is abnormal based on the pressure data. Finally, if it is abnormal, the target light wave corresponding to the respiration state is transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons. The mouse respiration can be efficiently monitored and adjusted, thereby helping experimenters to have a more comprehensive understanding of the relationship between respiration and neuronal activity.
[0046] In one embodiment, a mouse breathing monitoring and adjustment system is provided, and its internal structure diagram can be shown as follows: Figure 3As shown. The mouse respiratory monitoring and adjustment system includes a processor, a memory, a network interface, a display screen and an input device connected through a system bus. Among them, the processor of the mouse respiratory monitoring and adjustment system is used to provide computing and control capabilities. The memory of the mouse respiratory monitoring and adjustment system includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the operation of the operating system and the computer program in the non-volatile storage medium. The network interface of the mouse respiratory monitoring and adjustment system is used to communicate with an external server through a network connection. When the computer program is executed by the processor, the functions or steps of a mouse respiratory monitoring and adjustment method are realized.
[0047] In one embodiment, a mouse breathing monitoring and adjustment system is provided, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor implements the following steps when executing the computer program:
[0048] Obtain monitoring signals;
[0049] In response to the monitoring signal, obtaining pressure data of the pressure sensor;
[0050] determining, based on the pressure data, whether the respiratory state of the target mouse in the whole body plethysmography device is abnormal;
[0051] If it is abnormal, the target light waves corresponding to the breathing state will be transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons.
[0052] The mouse respiration monitoring and adjustment method of the present embodiment obtains the monitoring signal and responds to the monitoring signal to obtain the pressure data of the pressure sensor, and then determines whether the respiration state of the target mouse in the whole body plethysmography device is abnormal based on the pressure data. Finally, if it is abnormal, the target light wave corresponding to the respiration state is transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons. The mouse respiration can be efficiently monitored and adjusted, thereby helping experimenters to have a more comprehensive understanding of the relationship between respiration and neuronal activity.
[0053] In one embodiment, a computer-readable storage medium is provided, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the following steps are implemented:
[0054] Obtain monitoring signals;
[0055] In response to the monitoring signal, obtaining pressure data of the pressure sensor;
[0056] determining, based on the pressure data, whether the respiratory state of the target mouse in the whole body plethysmography device is abnormal;
[0057] If it is abnormal, the target light waves corresponding to the breathing state will be transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons.
[0058] The mouse respiration monitoring and adjustment method of the present embodiment obtains the monitoring signal and responds to the monitoring signal to obtain the pressure data of the pressure sensor, and then determines whether the respiration state of the target mouse in the whole body plethysmography device is abnormal based on the pressure data. Finally, if it is abnormal, the target light wave corresponding to the respiration state is transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons. The mouse respiration can be efficiently monitored and adjusted, thereby helping experimenters to have a more comprehensive understanding of the relationship between respiration and neuronal activity.
[0059] It should be noted that the functions or steps that can be implemented by the above-mentioned computer-readable storage medium or mouse respiratory monitoring and adjustment system can be found in the corresponding descriptions of the server side and the client side in the aforementioned method embodiment. To avoid repetition, they will not be described one by one here.
[0060] Those skilled in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be completed by instructing the relevant hardware through a computer program, and the computer program can be stored in a non-volatile computer-readable storage medium. When the computer program is executed, it can include the processes of the embodiments of the above-mentioned methods. Among them, any reference to memory, storage, database or other media used in the embodiments provided in this application can include non-volatile and / or volatile memory. Non-volatile memory can include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM) or flash memory. Volatile memory can include random access memory (RAM) or external cache memory. As an illustration and not limitation, RAM is available in many forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM).
[0061] The embodiments described above are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention, and should all be included in the protection scope of the present invention.
Claims
1. A method for monitoring and adjusting the respiration of mice, It is characterized in that The invention is applied to a mouse respiratory monitoring and adjustment system, wherein the mouse respiratory monitoring and adjustment system comprises: a whole body plethysmography device, a wireless optogenetic device, a feedback device and an integrated microprocessor device, wherein the whole body plethysmography device is provided with a pressure sensor, and the whole body plethysmography device, the wireless optogenetic device and the feedback device are electrically connected to the integrated microprocessor device respectively; The mouse respiratory monitoring and adjustment method comprises: Obtain monitoring signals; In response to the monitoring signal, obtaining pressure data of the pressure sensor; determining, based on the pressure data, whether the respiratory state of the target mouse in the whole body plethysmography device is abnormal; If it is abnormal, the target light waves corresponding to the breathing state will be transmitted to the neurons carrying the light-sensitive gene of the target mouse to inhibit or excite the neurons.
2. The method for monitoring and adjusting mouse respiration according to claim 1, It is characterized in that The step of determining whether the respiratory state of the target mouse in the whole body plethysmography device is abnormal based on the pressure data comprises: Processing the pressure data to obtain a breathing curve, and calculating a current breathing parameter based on the breathing curve; The current breathing parameters are compared with preset normal mouse breathing parameters to determine whether the breathing state is abnormal.
3. The mouse respiratory monitoring and adjustment method according to claim 1, It is characterized in that The whole body plethysmography device comprises: a closed body scanning box, and the closed body scanning box is provided with the pressure sensor.
4. The mouse respiration monitoring and adjustment method according to claim 3, It is characterized in that The pressure sensor is connected to a pressure conversion module, the pressure conversion module includes a pressure transducer and an amplifier, and the pressure conversion module is connected to the integrated microprocessor device.
5. The mouse respiration monitoring and adjustment method according to claim 1, It is characterized in that The wireless optogenetic device is arranged on the head of the target mouse.
6. A mouse breathing monitoring and adjustment system, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, It is characterized in that When the processor executes the computer program, the steps of the mouse respiration monitoring and adjustment method according to any one of claims 1 to 5 are implemented.
7. A computer-readable storage medium storing a computer program, It is characterized in that When the computer program is executed by a processor, the steps of the mouse respiration monitoring and adjustment method according to any one of claims 1 to 5 are implemented.