Kit for auxiliary detection of radiation-induced pulmonary fibrosis

Abstract

The invention discloses a kit for auxiliary detection of radiation-induced pulmonary fibrosis, and belongs to the technical field of biological detection, the kit comprises a box body and a slice sample, a reagent rack is arranged in the box body, and a fixed table is arranged in the box body. According to the invention, through the cooperation of the cylinder and the dropper, the dropper which can be rotatably switched can be used for automatically conveying a slice sample to the center of the slice sample under the dropper to realize automatic constant-speed dropping of the reagent, and in the process of dropping the reagent, the pressure is increased through downward movement of the pressing rod and the pressure is absorbed and released in a delayed manner by the driving ring; after a reagent is dropwise added, the delayed slice sample can be driven to rotate, so that the reagent at the center slowly moves and diffuses towards the edge under the action of centrifugal force, the reagent is ensured to be in full contact with the slice sample, the mixing effect of the reagent and the slice sample is improved, manual operation is reduced, and the stability and action effect of dropwise adding the reagent are improved; and the accuracy of subsequent detection and the overall processing efficiency of the slice sample are further improved.

Claims

1. A kit for assisting the detection of radiation-induced pulmonary fibrosis, comprising a box body (100) and a slice sample (500), wherein a reagent rack (101) is arranged inside the box body (100), characterized in that: A fixed platform (104) is arranged inside the box body (100), a pressure rod (108) is sleeved at the center of the fixed platform (104), a rotating seat (105) is rotatably connected to the top of the fixed platform (104), a detection slot (102) is provided inside the box body (100), a bracket (200) is slidably connected inside the detection slot (102), the bracket (200) is connected to a linear motor (201), a sensing area (110) matched with the linear motor (201) is arranged inside the box body (100), a slide rail is arranged between the linear motor (201) and the box body (100), and a dyeing mechanism is arranged inside the box body (100); The dyeing mechanism comprises a cylinder (103) arranged inside a box body (100); a plurality of groups of liquid storage chambers (106) are arranged in a ring shape on the side wall of the rotary seat (105); a dropper (107) is arranged at one end of the liquid storage chamber (106) away from the rotary seat (105); a piston rod (111) is arranged inside the dropper (107); a spring is arranged between the piston rod (111) and the inside of the dropper (107); a liquid outlet is arranged at the bottom of the dropper (107); the dropper (107) is communicated with the inside of the liquid storage chamber (106); a pressing plate (109) corresponding to the piston rod (111) is arranged at the top of the pressing rod (108); and a driving mechanism for driving the slice sample (500) to rotate and a cleaning mechanism for processing the slice sample (500) after dyeing are arranged inside the box body (100).

2. A kit for assisting the detection of radiation-induced pulmonary fibrosis according to claim 1, characterized in that: The driving mechanism comprises a driving sleeve (400), a return spring (401) and a buffer ring (402) arranged inside the box body (100); a liquid pressure transmission medium is arranged inside the driving sleeve (400); the bottom of the pressure rod (108) extends into the driving sleeve (400) and is provided with a piston ring; a return spring (401) is arranged between the piston ring at the bottom of the pressure rod (108) and the driving sleeve (400); the bottom of the driving sleeve (400) is connected to a liquid supply joint (404) through a pipeline; two groups of openings are provided at the end of the liquid supply joint (404); the buffer ring (402) is provided with a connecting groove (412) connecting the end opening of the liquid supply joint (404) and the inside of the driving ring (403); a slider 1 (405) is arranged inside the buffer ring (402); an arc spring (412) is arranged between the slider 1 (405) and the buffer ring (402). 406), a second slider (407) is sleeved inside the driving ring (403), and the second slider (407) is connected to an inner gear ring (408); the box body (100) is rotatably connected to a driving shaft (302); a driving seat (300) and a connecting tube (301) are arranged on the top of the driving shaft (302); a detection base (202) is rotatably connected to the inside of the bracket (200); the slice sample (500) is snap-fitted to the center of the top of the detection base (202); the bottom of the detection base (202) is designed to correspond to the connecting tube (301); a driving gear (303) is arranged on the side wall of the driving shaft (302); a transmission gear (304) matching the driving gear (303) and the inner gear ring (408) is arranged inside the box body (100); a buffer assembly is arranged inside the buffer ring (402); and a delayed start assembly is arranged at the bottom of the driving ring (403).

3. A kit for assisting the detection of radiation-induced pulmonary fibrosis according to claim 2, characterized in that: The cleaning mechanism comprises a cleaning plate (203) arranged on the top of the detection base (202); a hole corresponding to the bottom liquid outlet of the dropper (107) is provided at the center of the cleaning plate (203); a fan-shaped groove corresponding to the connecting tube (301) is provided at the bottom of the detection base (202); a liquid path tube (207) is provided inside the detection base (202); a nozzle (205) is arranged on the top of the liquid path tube (207) in an annular arrangement surrounding the hole of the cleaning plate (203); the nozzle (205) is opened toward the detection base (202); The center of the measuring base (202) is tilted, the connecting tube (301) and the driving shaft (302) are provided with a pipeline matching the liquid circuit tube (207), the bottom of the driving ring (403) is provided with a liquid supply cavity (409), the bottom of the sliding block (407) is provided with a sliding block (413) matching the liquid supply cavity (409), and the inside of the liquid supply cavity (409) is provided with two groups of hoses with one-way valves, one group of hoses is connected to an external device for liquid supply, and the other group of hoses is connected to the inside of the driving shaft (302).

4. A kit for assisting the detection of radiation-induced pulmonary fibrosis according to claim 3, characterized in that: Two magnetic blocks two (206) are symmetrically arranged at the bottom of the bracket (200), and a magnetic block one (204) corresponding to the magnetic block two (206) is arranged at the bottom of the detection base (202).

5. A kit for assisting the detection of radiation-induced pulmonary fibrosis according to claim 2, characterized in that: The buffer assembly includes an outer arc-shaped slide bar (410) and an inner arc-shaped slide bar (411) slidably connected inside the buffer ring (402). Two groups of protrusions extending to the inner cavity of the buffer ring (402) and cooperating with the slider one (405) are arranged at the top of the outer arc-shaped slide bar (410). Two groups of protrusions extending to the inner cavity of the drive ring (403) and cooperating with the slider two (407) are arranged at the bottom of the inner arc-shaped slide bar (411). The outer arc-shaped slide bar (410) and the inner arc-shaped slide bar (411) extend into the buffer ring (402) and are provided with "C"-shaped stoppers, and the openings of the stoppers of the outer arc-shaped slide bar (410) and the inner arc-shaped slide bar (411) face each other. A set of communication hole groups one (414) matching the communication groove (412) is arranged in the stopper of the outer arc-shaped slide bar (410), and a set of communication hole groups two (415) matching the communication groove (412) is arranged in the stopper of the inner arc-shaped slide bar (411).

6. A kit for assisting the detection of radiation-induced pulmonary fibrosis according to claim 5, characterized in that: The set of communication hole groups one (414) arranged on the outer arc-shaped slide bar (410) is designed in a non-overlapping staggered manner in the vertical direction, and the set of communication hole groups two (415) arranged on the inner arc-shaped slide bar (411) is designed to overlap in the vertical direction.

7. A kit for assisting the detection of radiation-induced pulmonary fibrosis according to claim 5, characterized in that: Both ends of the outer arc-shaped slide bar (410) and the inner arc-shaped slide bar (411) are designed as magnets. Two groups of chutes are arranged inside the buffer ring (402) and cooperate with the outer arc-shaped slide bar (410) and the inner arc-shaped slide bar (411) respectively, and magnets corresponding to the outer arc-shaped slide bar (410) and the inner arc-shaped slide bar (411) are also designed at both ends of the chute.

8. A kit for assisting the detection of radiation-induced pulmonary fibrosis according to claim 2, characterized in that: The delay start-up assembly includes a delay cavity (416) communicated with the inside of the drive ring (403). A slider four (417) is sleeved inside the delay cavity (416). The slider four (417) is designed as a magnet. Magnetic blocks three (419) corresponding to the slider four (417) are designed at the top and bottom of the inner cavity of the delay cavity (416). An exhaust hole (418), an air inlet hole (420) and an exhaust valve (424) are arranged on the side wall of the delay cavity (416). An L-shaped channel corresponding to the air inlet hole (420) is arranged inside the slider four (417). A rotary cover (422) is rotatably connected to the bottom of the delay cavity (416). An air inlet groove one (421) is arranged at the bottom of the delay cavity (416), and an air inlet groove two (423) corresponding to the rotary cover (422) is arranged on the rotary cover (422).

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