Immunohistochemical reaction system and method

Abstract

The invention discloses an immunohistochemical reaction system and method, relates to the technical field of biomedical treatment, and mainly aims to solve the problem of poor staining effect of existing immunohistochemical. Comprising a tissue sample incubation area, a mechanical arm, a glass slide clamping piece and a controller, the tissue sample incubation area comprises multiple layers of reaction trays, each reaction tray comprises a plurality of glass slide heating areas, and heaters aligned with glass slide grooves in the glass slide clamping piece are arranged in the glass slide heating areas; the reaction tray enters and exits the tissue sample incubation area through conveying equipment arranged on the two sides of the tissue sample incubation area; the controller is used for determining a target glass slide identifier of the target tissue glass slide and driving the mechanical arm to place the glass slide clamping piece bearing the target tissue glass slide in the target glass slide heating area according to the target glass slide identifier; and controlling a target heater corresponding to the target glass slide heating area to heat according to a preset heating threshold.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to an immunohistochemical reaction system and method. Background Art

[0002] Immunohistochemistry (IHC) is a practical biological experimental technique that uses the principle of specific binding of antigens and antibodies, enzymes, proteins or chemical dyes, to display macromolecular proteins or gene expression in vivo in situ on tissue sections or cell slices. It is widely used in the pathological diagnosis of various diseases such as tumors and inflammation. With the development of digital pathology technology, in order to better perform pathological diagnosis, automated immunohistochemical staining equipment has been put into operation to identify and analyze tissues after the reaction. Among them, when immunohistochemical staining of tissue samples on slides is performed, it is necessary to titrate the reagents, and then heat and incubate at a constant temperature. At present, when incubating tissue samples on slides, it is necessary to place the slide in a fixed position to absorb the liquid in the reagent bottle and then titrate the reagent on the slide, and then place each slide in the incubation area for reaction, and usually install a heater to heat all slides uniformly. However, placing slides one by one will generate a large number of robot arm linkage operations, which greatly increases the operating cost of the equipment. In addition, a heater in the incubation area cannot ensure that the heating temperature of the slides at various positions is consistent, which greatly reduces the immunohistochemical staining effect. Summary of the invention

[0003] In view of this, the present application provides an immunohistochemical reaction system and method, the main purpose of which is to solve the problem of poor immunohistochemical staining effect in existing immunohistochemical techniques.

[0004] According to one aspect of the present application, an immunohistochemical reaction system is provided, comprising: Tissue sample incubation area, robotic arm, slide holder, controller, The tissue sample incubation area includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating areas, the slide heating areas are provided with heaters aligned with the slide slots on the slide clamping member, and the reaction tray enters and exits the tissue sample incubation area through a conveying device provided on both sides of the tissue sample incubation area; The mechanical arm is used to clamp the slide holder and place it in the slide heating area on the reaction tray extending out of the tissue sample incubation area to perform immunohistochemical staining; The slide holder is used to load a plurality of tissue slides; The controller is used to determine the target slide identifier of the target tissue slide, and drive the robotic arm to place the slide clamp carrying the target tissue slide in the target slide heating zone according to the target slide identifier; and control the target heater corresponding to the target slide heating zone to heat according to a preset heating threshold.

[0005] Furthermore, the tissue sample incubation area further comprises a motor, which is electrically connected to the conveying device and is used to drive the conveying device according to the extension instruction or the entry instruction of the controller; the mechanical arm is provided with a clamping claw assembly; the slide holder comprises a slide holder body and a slide holder clamp, the slide holder clamp is provided on one side of the slide holder body, and the two sides of the slide holder clamp are provided with a card slot, and the card slot and the clamping claw can be engaged and clamped; The controller is further used to query the slide heating zone in the reaction tray that is in an idle state, and determine the target slide heating zone according to the idle state; The controller is further used to determine a conveying path for the robot arm to clamp the target glass slide clamp, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target glass slide heating zone, drive the robot arm to clamp the glass slide clamp according to the conveying path and place it in the target glass slide heating zone. The conveying path is a route determined based on the placement position of the target glass slide heating zone and the clamping position of the target glass slide clamp.

[0006] Furthermore, the system also includes a dripping device for sucking and dripping out medicine; the tissue sample incubation area also includes a medicine refrigeration area and a medicine storage area arranged on the top layer of the reaction tray; the medicine refrigeration area is used to provide cold air to the reaction tray; the medicine storage area includes a plurality of medicine storage slots for storing medicine bottles; Wherein, the controller is also used to determine the target medicine and the target dripping amount according to the target slide identification, and drive the dripping device to absorb the medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating area.

[0007] Furthermore, the medicine storage tank includes a first medicine storage tank and a second medicine storage tank, the first medicine storage tank is arranged in a honeycomb shape, and the second medicine storage tank is arranged in parallel according to preset rows and preset columns; The controller is specifically used to determine the suction position of the dripping device according to the target medicine, and determine the dripping position of the dripping device in combination with the target slide heating area; and generate a driving route of the dripping device according to the suction position and the dripping position.

[0008] Furthermore, partitions are provided on both sides of the glass slide heating zone for stabilizing the glass slide clamp, and the height of the partitions is greater than the thickness of the glass slide clamp.

[0009] Furthermore, a limit baffle is disposed on the side of the reaction tray, and a frame baffle aligned with the limit baffle is disposed on the inner side of the shell of the tissue sample incubation area to limit the extension length of the reaction tray.

[0010] Furthermore, a drainage hole is provided on the bottom surface of the reaction tray, and a drip groove is slidably connected to the bottom surface at a position corresponding to the drainage hole. The drip groove has a notch, and a drainage pipe mouth is provided at the drainage hole. The drainage pipe mouth extends toward the drip groove and is provided at the far end of the notch.

[0011] According to another aspect of the present application, a reaction method for immunohistochemical staining is provided, comprising: When a target slide holder is loaded with a plurality of tissue slides, a target slide identifier of the target slide holder is obtained through a controller, and a target slide heating zone in a tissue sample incubation zone is determined based on the target slide identifier, wherein the tissue sample incubation zone includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating zones, a heater aligned with a slide groove on the slide holder is provided in the slide heating zone, and the reaction tray enters and exits the tissue sample incubation zone through a conveying device provided on both sides of the tissue sample incubation zone; The controller drives the robotic arm to place the slide holder carrying the target tissue slide in the target slide heating zone according to the target slide identifier, and controls the target heater on the target slide heating zone to heat according to a preset heating threshold.

[0012] By means of the above technical solution, the technical solution provided by the embodiment of the present application has at least the following advantages: The present application provides an immunohistochemical reaction system and method. Compared with the prior art, the reaction system in the embodiment of the present application includes: a tissue sample incubation area, a robotic arm, a slide holder, and a controller. The tissue sample incubation area includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating areas, the slide heating areas are provided with heaters aligned with the slide grooves on the slide holder, the reaction tray enters and exits the tissue sample incubation area through a conveying device provided on both sides of the tissue sample incubation area; the robotic arm is used to clamp the slide holder and place it on the slide heating area on the reaction tray extending from the tissue sample incubation area to perform immunohistochemical reaction. The invention relates to immunohistochemical staining; the slide holder is used to load a plurality of tissue slides; the controller is used to determine the target slide identifier of the target tissue slide, and drive the mechanical arm to place the slide holder carrying the target tissue slide in the target slide heating zone according to the target slide identifier; the target heater corresponding to the target slide heating zone is controlled to heat according to a preset heating threshold, thereby greatly increasing the loading capacity of the slides in the tissue sample incubation zone and reducing the transmission effect of the mechanical arm in a limited space. By configuring the heating zone in each slide heating zone, the tissue sample is accurately heated in the tissue sample incubation zone, thereby improving the effectiveness of immunohistochemical staining.

[0013] Usually, one heater is installed to heat all slides uniformly. However, placing slides one by one will generate a large number of robot arm linkage operations, which greatly increases the equipment operation cost, and one heater in the incubation area cannot ensure that the heating temperature of slides at various positions is consistent, which greatly reduces the immunohistochemical staining effect.

[0014] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the detailed description of the preferred embodiments below. The accompanying drawings are only for the purpose of illustrating the preferred embodiments and are not to be considered as limiting the present application. Also, the same reference symbols are used throughout the accompanying drawings to represent the same components. In the accompanying drawings: Figure 1 A structural block diagram of an immunohistochemical reaction system provided in an embodiment of the present application is shown; Figure 2 A schematic diagram of the structure of a tissue sample incubation area provided in an embodiment of the present application is shown; Figure 3A schematic diagram of the structure of a transmission motor provided in an embodiment of the present application is shown; Figure 4 A schematic diagram of the structure of a glass slide holder provided in an embodiment of the present application is shown; Figure 5 A schematic diagram of the structure of a clamping claw and a glass slide clamping member provided in an embodiment of the present application is shown; Figure 6 A schematic diagram of the structure of a dripping device provided in an embodiment of the present application is shown; Figure 7 A schematic diagram of the structure of a medicine refrigeration area and a medicine storage area provided in an embodiment of the present application is shown; Figure 8 A schematic diagram of a heating table structure provided in an embodiment of the present application is shown; Fig. 9 A schematic diagram of a limit stopper structure provided in an embodiment of the present application is shown; Fig.10 A schematic diagram of the structure of a drip tank provided in an embodiment of the present application is shown; Fig.11 A schematic diagram of an immunohistochemical reaction process provided in an embodiment of the present application is shown; Among them, there are tissue sample incubation area-1, robotic arm-2, slide clamp-3, controller-4, reaction tray-11, slide heating area-111, heater-112, conveying device-12, slide slot-31, motor-13, clamp-5, slide rack-32, slide rack chuck-33, card slot-34, dripping device-6, medicine refrigeration area-7, medicine storage area-8, medicine storage slot-81, first medicine storage slot-811, second medicine storage slot-812, heating table-113, partition-114, limit baffle-14, rack baffle-15, drainage hole-16, drip groove-17, drainage pipe mouth-161, and push baffle-18. DETAILED DESCRIPTION

[0016] The exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0017] The present application embodiment provides an immunohistochemical reaction system, such as Figure 1 The schematic diagram of the system structure shown in the figure, the system comprises: a tissue sample incubation area 1, a robotic arm 2, a slide holder 3, and a controller 4. Among them, Figure 2As shown, the tissue sample incubation area 1 includes a multi-layer reaction tray 11, and the reaction tray 11 includes a plurality of slide heating areas 111. The slide heating area 111 is provided with a heater 112 aligned with the slide groove 31 on the slide clamp 3. The reaction tray 11 enters and exits the tissue sample incubation area through a conveying device 12 provided on both sides of the tissue sample incubation area; The robot arm is used to clamp the glass slide holder 3 and place it in the glass slide heating area 111 on the reaction tray 11 extending out of the tissue sample incubation area 1 to perform immunohistochemical staining; The slide holder 3 is used to load a plurality of tissue slides; The controller 4 is used to determine the target slide identifier of the target tissue slide, and drive the robotic arm 2 to place the slide clamp 3 carrying the target tissue slide in the target slide heating zone 111 according to the target slide identifier; and control the target heater 112 corresponding to the target slide heating zone 111 to heat according to a preset heating threshold.

[0018] In the embodiment of the present application, in the medical field, immunohistochemical cell tissues, including but not limited to non-diseased tissues or diseased tissues in human organs, are sampled through a glass slide as a tissue sample. At this time, the glass slide groove 31 in the glass slide holder 3 can clamp the glass slide. A glass slide holder 3 includes multiple glass slide grooves 31, and correspondingly, multiple glass slides can be clamped. When the tissue sample needs to be heated and reacted, the glass slide is clamped and moved in the system by the robot arm 2. Among them, the tissue sample incubation area 1 includes a multi-layer reaction tray 11, which is arranged one by one in the tissue sample incubation area 1 according to the structure of each layer. Figure 2 As shown, the reaction tray 11 includes a plurality of glass slide heating zones 111, wherein the glass slide heating zones 111 are provided with heaters 112 aligned with the glass slide grooves 31 on the glass slide holders 3, so that after a glass slide holder 3 is directly placed on the glass slide heating zone 111, the aligned heating zones can directly heat the glass slides in the glass slide grooves 31. The heater 112 is provided on a bridge-shaped table at the bottom of the reaction tray 11, and a cavity is formed with the bridge-shaped table and the bottom for storing dripping medicines. At the same time, a drainage hole 16 is provided at a corner position of the reaction tray 11, such as Figure 2 As shown, the slide heating area 111 in each layer of the reaction tray 11 is arranged in the longitudinal direction, so that the reaction tray 11 can heat multiple slides within the maximum space limit. Figure 2As shown, the reaction tray 11 is also extended out of the tissue sample incubation area 1 through the conveying devices 12 arranged on both sides of the tissue sample incubation area 1, so that the reaction tray 11 can place the slide holder 3 or remove the slide holder 3 after extending out of the tissue sample incubation area 1.

[0019] It should be noted that the reaction system in the embodiment of the present application is triggered by the controller 4. At this time, the controller 4 can be a terminal processor or a server device, and data communication is performed with the mechanical arm 2, the motor 13 and other devices through the communication line. The embodiment of the present application is not specifically limited. Among them, the user can transmit the target carrier identification of the tissue slide on the slide holder 3 to the controller 4 by inputting or taking pictures. The shooting device can also be configured to take pictures of the tissue slide and then produce the corresponding target slide identification for the controller 4 to query and determine. The embodiment of the present application is not specifically limited. Furthermore, the controller 4 can determine the specific position of the slide heating zone 111 according to the target slide identification, that is, determine the target slide heating zone 111 to be placed. At this time, since the tissue sample incubation area 1 includes a multi-layer reaction tray 11, each layer of the reaction tray 11 includes a plurality of slide heating zones 111. When determining the target slide heating zone 111, the idle slide heating zones 111 can be queried one by one. The embodiment of the present application is not specifically limited. After determining the target slide heating zone 111, the controller 4 sends a control instruction to the robot arm 2 to control the continuing arm to move the held target slide clamp 3 to the target slide heating zone 111 for placement, and then control the target heater 112 on the target slide heating zone 111 to heat according to a preset heating threshold. The preset heating threshold includes the heating time and the heating temperature. For example, heating is performed at ±36.5 degrees for 1 day to simulate human body temperature, which is not specifically limited in the embodiments of the present application.

[0020] In addition, in the embodiment of the present application, since the reaction tray 11 is arranged layer by layer, in order to enable the reaction tray 11 to automatically and flexibly extend and retract to prevent the dry slice, the reaction tray 11 enters and exits the tissue sample incubation area 1 through the conveying device 12 arranged on both sides of the tissue sample incubation area 1. Figure 2 As shown, the conveying device 12 includes a shaft wheel and a conveying belt around the two shaft wheels. The motor 13 drives the rear shaft wheel to rotate, and drives the front shaft wheel to rotate through the conveying belt. The front shaft wheel is connected to the reaction tray 11 through transmission, and drives each reaction tray 11 to extend or retract. The time when the motor 13 stops rotating can be controlled by the controller 4 to control the extension and holding time of the reaction tray 11, which is not specifically limited in the embodiment of the present application.

[0021] In another embodiment of the present application, for further definition and explanation, the tissue sample incubation area 1 further includes a motor 13, such as Figure 3 As shown, it is electrically connected to the conveying device 12 and is used to drive the conveying device 12 according to the extension instruction or the entry instruction of the controller 4. The mechanical arm 2 is provided with a clamping claw 5 assembly; the slide holder 3 includes a slide holder body 32 and a slide holder chuck 33, the slide holder chuck 33 is arranged on one side of the slide holder body 32, and the two sides of the slide holder chuck 33 are provided with a card slot 34, and the card slot 34 can be engaged with the clamping claw 5 for clamping; The controller 4 is further used to query the slide heating area 111 in the reaction tray 11 which is in an idle state, and determine the target slide heating area 111 according to the idle state; The controller 4 is further used to determine a conveying path for the robot arm 2 to clamp the target glass slide clamp 3, and after driving the motor 13 to drive the conveying device 12 to extend the reaction tray 11 corresponding to the target glass slide heating zone 111, drive the robot arm 2 according to the conveying path to clamp the glass slide clamp 3 and place it in the target glass slide heating zone 111.

[0022] In order to accurately drive the reaction tray 11 to extend or retract through the controller 4, the tissue sample incubation area 1 further includes a motor 13, which is disposed on the back plate of each layer of the reaction tray 11. Figure 3 As shown, it is electrically connected to the conveying device 12 and is used to drive the conveying device 12 according to the extension instruction or the entry instruction of the controller 4. When the controller 4 determines the target slide heating zone 111 and the corresponding reaction tray 11, it sends a control instruction to the corresponding motor 13 to drive the extension or retraction. The embodiment of the present application does not make specific limitations. At the same time, in order to enable the robot arm 2 to stably hold the slide clamp 3 so that the robot arm 2 can flexibly clamp, a clamping claw 5 component is provided on the robot arm 2. At the same time, as Figure 4 As shown, in order to ensure that the glass slide holder 3 can be stably clamped by the clamping claw 5, the glass slide holder 3 includes a glass slide frame 32 and a glass slide frame clamp 33. The glass slide frame clamp 33 is arranged on one side of the glass slide frame 32. Slots 34 are provided on both sides of the glass slide frame clamp 33 so that the slots 34 and the clamping claw 5 can be engaged and clamped. At the same time, the glass slide frame 32 also includes an elastic clip 35, which is made of elastic metal, one end of which is fixed on the glass slide frame 32, and the other end clamps the glass slide.

[0023] It should be noted that, in order to make the slide heating areas 111 in each layer of the reaction tray 11 be used efficiently, the controller 4 may first query the idle slide heating areas in the reaction tray 11 to determine the target slide heating areas 111 from the idle slide heating areas 111. Specifically, the idle state may be determined based on the heating signal of the heater 112. For screening of all idle slide heating areas 111, the reaction trays 11 may be sorted from top to bottom, and then the idle slide heating areas 111 may be randomly selected from the topmost reaction tray 11. All slide heating areas 111 may also be numbered, and the idle slide heating areas 111 may be sorted in sequence according to the numbers. The embodiment of the present application does not make any specific limitation. After determining the target slide heating zone 111, in order to better control the robot arm 2 to operate in the system environment and improve the moving efficiency, the controller 4 determines the transmission path according to the target reaction tray 11 and the target slide heating zone 111, so that the robot arm 2 can clamp the slide clamp 3 and then move to the target slide heating zone 111 for placement. Among them, the transmission path is a route determined based on the placement position of the target slide heating zone 111 and the clamping position of the target slide clamp 3. The controller 4 determines the placement position of the target slide heating zone 111 and the clamping position of the target slide clamp 3 (for example, the position of the slide clamp 3 that has been repaired in the repair instrument) by loading the moving space coordinates of the robot arm 2, and then optimizes the placement position and the clamping position to obtain the transmission path. The path optimization method is not specifically limited in the embodiment of the present application.

[0024] In the embodiment of the present application, the execution code for generating the transmission path in the controller 4 can be set based on the coordinates provided in the robot arm 2 and the pre-written script code, and the embodiment of the present application does not make specific limitations.

[0025] In a specific embodiment, the slot 34 and the jaw 5 are designed to fit together. The slot 34 includes at least two slot surfaces, and the slot surfaces form a first angle. In contrast, the jaw 5 includes a first jaw side 51 and a second jaw side 52. Each jaw side is provided with a plurality of raised surfaces to form a jaw body 53. The raised surfaces form a second angle. At this time, the first angle is complementary to the second angle, so that the slot 34 and the jaw 5 are fit together and clamped by the first angle and the second angle. Figure 5 shown.

[0026] In another embodiment of the present application, in order to further define and illustrate, Figure 6As shown, the system further includes a dripping device 6 for sucking and dripping out medicine; the tissue sample incubation area 1 further includes a medicine refrigeration area 7 and a medicine storage area 88 arranged on the top layer of the reaction tray 11; the medicine refrigeration area 7 is used to provide cold air to the reaction tray 11; the medicine storage area 8 includes a plurality of medicine storage slots 81 for storing medicine bottles; The controller 4 is also used to determine the target medicine and the target dripping amount according to the target slide identification, and drive the dripping device 6 to absorb the medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating area 111.

[0027] In order to efficiently utilize the tissue sample incubation area 1 for immunohistochemical staining, the system further includes a dripping device 6 for sucking and dripping out the reagent. At the same time, in order to efficiently drip, the reagent refrigeration area 7 and the reagent storage area 8 on the top layer of the tissue sample incubation area 1 are Figure 7 As shown, at this time, the medicine refrigeration zone 7 is used to provide cold air to the reaction tray 11, and refrigerant can be used for refrigeration, which is not specifically limited in the embodiment of the present application. At the same time. The medicine storage area 88 includes a plurality of medicine storage slots 81 for storing medicine bottles, and different medicines can be placed in different medicine bottles, which is not specifically limited in the embodiment of the present application. At this time, in order to make the medicine titration accurate and effective, the controller 4 first determines the target medicine and the corresponding target drop amount according to the target slide mark, and then drives the dripping device 6 to absorb the medicine from the target medicine bottle, and then drips the medicine to the target tissue slide on the target slide heating zone 111. Among them, since the slide mark is information for the identity of the standard tissue sample, the controller 4 can pre-store medicines and drop amounts matching different slide marks for query and determination, which is not specifically limited in the embodiment of the present application.

[0028] In another embodiment of the present application, for further definition and explanation, the medicine storage slot 81 includes a first medicine storage slot 811 and a second medicine storage slot 812, wherein the first medicine storage slot 811 is arranged in a honeycomb shape, and the second medicine storage slot 812 is arranged in parallel according to preset rows and preset columns; The controller 4 is further specifically used to determine the suction position of the dripping device 6 according to the target medicine, and determine the dripping position of the dripping device 6 in combination with the target slide heating zone 111; and generate a driving route of the dripping device 6 according to the suction position and the dripping position.

[0029] In order to place more reagents in the tissue sample incubation area 1, the reagent storage tank 81 includes a first reagent storage tank 811 and a second reagent storage tank 812. The first reagent storage tank 811 and the second reagent storage tank 812 store reagent bottles with different reagent volumes, respectively. Figure 7As shown, the first medicine storage slots 811 are arranged in a honeycomb shape, and the second medicine storage slots 812 are arranged in parallel according to preset rows (such as 2 rows) and preset columns (such as 8 columns) to distinguish the storage of medicines of different doses, which is not specifically limited in this embodiment of the present application.

[0030] Since the storage space of the medicine bottles is increased, the storage capacity of the medicine bottles is increased. In order to enable the dripping device 6 to drip more efficiently and accurately, the controller 4 determines the target medicine and the target dripping amount according to the target slide mark. Specifically, the corresponding dose mapping table can be pre-written in the controller 4, and the correspondence between different slide marks and different medicines and different dripping amounts can be pre-stored, so that the controller 4 can accurately take medicine for storing the medicines and dripping amounts required for the reaction of different slide marks. The embodiment of the present application does not make specific restrictions. In addition, when controlling the dripping device 6, the controller 4 can drive the dripping device 6 to drip the target medicine from the target medicine bottle, and drive the dripping device 6 to drip the medicine to the target tissue slide on the target slide heating zone 111, and then send a heating instruction to the heating zone of the target slide heating zone 111. The embodiment of the present application does not make specific restrictions.

[0031] It should be noted that the dripping device 6 in the embodiment of the present application includes a needle, an air pipe and an air pump. After the air pump is started, the dripping device 6 is sucked and dripped from the needle through the air pipe. In addition, the dripping device 6 can be set on a bracket with a conveyor belt to achieve control such as up and down movement and extension, which is not specifically limited in the embodiment of the present application.

[0032] In another embodiment of the present application, for further definition and explanation, partitions 114 are provided on both sides of the slide heating zone 111 for stabilizing the slide clamp 3 , and the height of the partitions 114 is greater than the thickness of the slide clamp 3 .

[0033] In order to enable the slide holder 3 to be stably placed in the slide heating area 111 of the reaction tray 11, the slide heating area 111 includes a heating table 113, a heater 112 is arranged on the bridge-shaped heating table 113, and the heating table 113 can be made of heat-conducting metal. At this time, partitions 114 are arranged on both sides of the heating table 113. Figure 8 As shown, it is used to stabilize the glass slide holder 3. At this time, the height of the partition 114 is greater than the thickness of the glass slide holder 3, which is not specifically limited in the embodiment of the present application.

[0034] In another embodiment of the present application, for further definition and explanation, a limit baffle 14 is provided on the side of the reaction tray 11 , and a frame baffle 15 aligned with the limit baffle 14 is provided on the inner side of the shell of the tissue sample incubation area 1 to limit the extension length of the reaction tray 11 .

[0035] In order to ensure that the reaction tray 11 is sufficiently safe during the extension and retraction process, thereby ensuring the effective reaction of the tissue sample, a limit stopper 14 is fixedly provided on the side of the reaction tray 11. Fig. 9 As shown, correspondingly, a frame baffle 15 aligned with the limit baffle 14 is provided on the inner side of the shell of the tissue sample incubation area 1 to limit the extension length of the reaction tray 11. The frame baffle 15 is formed after a track hole is opened on each side frame of the tissue sample incubation area 1, and the limit baffle 14 moves and limits the position in the track hole. The limit baffle 14 may include a rear limit baffle 141 and a front limit baffle 142, so as to limit the position of the reaction tray 11 during the extension and retraction process, which is not specifically limited in the embodiment of the present application.

[0036] In another embodiment of the present application, for further limitation and explanation, a drainage hole 16 is provided on the bottom surface of the reaction tray 11, and a drip groove 17 is slidably connected to the drainage hole 16 on the bottom surface, and the drip groove 17 is provided with a notch, and a drainage pipe mouth 161 is provided at the drainage hole 16, and the drainage pipe mouth 161 extends toward the drip groove 17 and is arranged at the far end of the notch.

[0037] When the tissue sample is reacting, the reagents titrated on the glass slide may flow into the reaction tray 11. In order to prevent the environmental humidity of the tissue sample incubation area 1 from being too high and affecting the reaction effect, each layer of the reaction tray 11 is provided with a drainage hole 16. Fig.10 As shown, the drainage hole 16 is preferably opened at the edge of the reaction tray 11, and the reaction tray 11 is made so that the horizontal plane at the drainage hole 16 is the lowest, and the residual liquid flows out of the drainage hole 16, and a drip groove 17 is slidably arranged on the bottom surface of the reaction tray 11 in a position aligned with the drainage hole 16. Among them, the drip groove 17 is arranged at the bottom of the reaction tray 11 through a guide rail. At this time, a drainage pipe opening 161 is arranged at the drainage hole 16, and the drainage pipe opening 161 extends toward the drip groove 17 and is arranged at a distance from the far end of the groove, that is, when the reaction tray 11 is extended, the drainage pipe opening 161 pushes one side of the drip groove 17 to slide out, and after the reaction tray 11 is extended, the drainage pipe opening 161 still discharges liquid into the drip groove 17, and the embodiment of the present application does not make specific limitations. At the same time, a push baffle 18 is fixedly connected to a position corresponding to one side of the drip groove 17 on the bottom surface of the reaction tray 11, and is used to push the drip groove 17 back to the initial position after the reaction tray 11 is retracted into the tissue sample incubation area 1. At this time, the initial position is the position where the drip groove 17 is aligned with the outer side of the reaction tray 11. The length of the drip groove 17 is preferably half the width of the reaction tray 11, which is not specifically limited in the embodiment of the present application.

[0038] In addition, since the drip groove 17 has a notch at the beginning, the residual liquid dripping from the upper layer of the reaction tray 11 will drip into the next layer of the reaction tray 11 along the notch of the drip groove 17, and finally drip into the bottom tray. At this time, the drainage pipe port 161 of the bottom tray can be directly connected to the waste liquid bucket, which is not specifically limited in the embodiment of the present application.

[0039] In addition, it should be noted that materials not specifically described in the embodiments of the present application are all metals, without specific limitation.

[0040] The present application provides an immunohistochemical reaction system, which greatly increases the capacity of slides in the tissue sample incubation area and reduces the transmission effect of the robot arm in a limited space. By configuring a heating zone in each slide heating zone, the tissue sample is accurately heated in the tissue sample incubation area, thereby improving the effectiveness of immunohistochemical staining.

[0041] Furthermore, as a response to the above Figure 1 The embodiment of the present application provides a method for immunohistochemical staining, such as Figure 3 As shown, the method includes: 101. When a target slide holder is loaded with a plurality of tissue slides, a target slide identifier of the target slide holder is obtained through a controller, and a target slide heating zone in a tissue sample incubation zone is determined based on the target slide identifier, wherein the tissue sample incubation zone includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating zones, a heater aligned with a slide groove on the slide holder is disposed in the slide heating zone, and the reaction tray enters and exits the tissue sample incubation zone through a conveying device disposed on both sides of the tissue sample incubation zone; 102. Drive the robot arm to place the slide holder carrying the target tissue slide in the target slide heating zone according to the target slide identifier through the controller, and control the target heater on the target slide heating zone to heat according to a preset heating threshold.

[0042] In the embodiment of the present application, the user can feed the slide holder holding multiple tissue slides through the material bin, and the controller can determine whether the feeding is completed through the photoelectric switch in the material bin, that is, as a trigger basis for triggering the target slide holder to load the tissue slide, the controller further obtains the target slide identifier of the target slide holder, and determines the target slide heating zone in the tissue sample incubation zone based on the target slide identifier. Furthermore, the controller drives the mechanical arm to place the slide holder carrying the target tissue slide in the target slide heating zone by sending a control instruction, and controls the target heater on the target slide heating zone to heat the target slide heating zone through the heating instruction carrying a preset heating threshold, so as to achieve the purpose of immunohistochemical staining of the tissue sample.

[0043] In another embodiment of the present application, for further definition and explanation, the tissue sample incubation area further includes a motor, electrically connected to the conveying device, for driving the conveying device according to the extension instruction or the entry instruction of the controller; querying the slide heating zone in the reaction tray which is in an idle state through the controller, and determining the target slide heating zone according to the idle state; The controller determines a conveying path for the robot arm to clamp the target glass slide holder, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target glass slide heating zone, the robot arm is driven according to the conveying path to clamp the glass slide holder and place it in the target glass slide heating zone. The conveying path is a route determined based on the placement position of the target glass slide heating zone and the clamping position of the target glass slide holder.

[0044] In another embodiment of the present application, for further definition and explanation, the tissue sample incubation area further includes a medicine refrigeration area and a medicine storage area arranged on the top layer of the reaction tray; the medicine refrigeration area is used to provide cold air to the reaction tray; the medicine storage area includes a plurality of medicine storage slots for storing medicine bottles; the medicine storage slots include a first medicine storage slot and a second medicine storage slot, the first medicine storage slots are arranged in a honeycomb shape, and the second medicine storage slots are arranged in parallel according to preset rows and preset columns; The controller determines the target medicine and the target dripping amount according to the target slide mark, determines the suction position of the dripping device for sucking and dripping the medicine according to the target medicine, and determines the dripping position of the dripping device in combination with the target slide heating zone; After the controller generates a driving route for the dripping device according to the suction position and the dripping position, the dripping device is driven to absorb medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating zone.

[0045] The present application provides a reaction method for immunohistochemical staining, which greatly increases the capacity of slides in a tissue sample incubation area and reduces the transmission effect of a robotic arm in a limited space. By configuring a heating zone in each slide heating zone, tissue samples are accurately heated in the tissue sample incubation area, thereby improving the effectiveness of immunohistochemical staining.

[0046] Obviously, those skilled in the art should understand that the above modules or steps of the present application can be implemented by a general computing device, they can be concentrated on a single computing device, or distributed on a network composed of multiple computing devices, and optionally, they can be implemented by a program code executable by a computing device, so that they can be stored in a storage device and executed by the computing device, and in some cases, the steps shown or described can be executed in a different order from that herein, or they can be made into individual integrated circuit modules, or multiple modules or steps therein can be made into a single integrated circuit module for implementation. Thus, the present application is not limited to any specific combination of hardware and software.

[0047] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An immunohistochemical reaction system, characterized in that: include: Tissue sample incubation area, robotic arm, slide holder, controller, The tissue sample incubation area includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating areas, the slide heating areas are provided with heaters aligned with the slide slots on the slide clamping member, and the reaction tray enters and exits the tissue sample incubation area through a conveying device provided on both sides of the tissue sample incubation area; The robotic arm is used to clamp the slide holder and place it in the slide heating area on the reaction tray extending out of the tissue sample incubation area to perform immunohistochemical staining; The slide holder is used to load a plurality of tissue slides; The controller is used to determine the target slide identification of the target tissue slide, and drive the mechanical arm to place the slide clamp carrying the target tissue slide in the target slide heating zone according to the target slide identification; The target heater corresponding to the target slide heating zone is controlled to heat according to a preset heating threshold.

2. The system according to claim 1, characterized in that The tissue sample incubation area also includes a motor, which is electrically connected to the conveying device and is used to drive the conveying device according to the extension instruction or the entry instruction of the controller; the mechanical arm is provided with a clamping claw assembly; the slide holder includes a slide holder body and a slide holder chuck, the slide holder chuck is provided on one side of the slide holder body, and the two sides of the slide holder chuck are provided with a card slot, and the card slot and the clamping claw can be engaged and clamped; The controller is further used to query the slide heating zone in the reaction tray that is in an idle state, and determine the target slide heating zone according to the idle state; The controller is further used to determine a conveying path for the robot arm to clamp the target glass slide clamp, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target glass slide heating zone, drive the robot arm to clamp the glass slide clamp according to the conveying path and place it in the target glass slide heating zone. The conveying path is a route determined based on the placement position of the target glass slide heating zone and the clamping position of the target glass slide clamp.

3. The system according to claim 1, characterized in that The system further includes a dripping device for sucking and dripping out medicine; the tissue sample incubation area further includes a medicine refrigeration area and a medicine storage area arranged on the top layer of the reaction tray; the medicine refrigeration area is used to provide cold air to the reaction tray; the medicine storage area includes a plurality of medicine storage slots for storing medicine bottles; Wherein, the controller is also used to determine the target medicine and the target dripping amount according to the target slide identification, and drive the dripping device to absorb the medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating area.

4. The system according to claim 3, characterized in that The medicine storage tank includes a first medicine storage tank and a second medicine storage tank, the first medicine storage tank is arranged in a honeycomb shape, and the second medicine storage tank is arranged in parallel according to preset rows and preset columns; The controller is specifically used to determine the suction position of the dripping device according to the target medicine, and determine the dripping position of the dripping device in combination with the target slide heating area; and generate a driving route of the dripping device according to the suction position and the dripping position.

5. The system according to claim 1, characterized in that Partitions are arranged on both sides of the glass slide heating zone to stabilize the glass slide clamp, and the height of the partitions is greater than the thickness of the glass slide clamp.

6. The system according to claim 1, characterized in that A limit baffle is disposed on the side of the reaction tray, and a frame baffle aligned with the limit baffle is disposed on the inner side of the shell of the tissue sample incubation area to limit the extension length of the reaction tray.

7. The system according to claim 1, characterized in that A drainage hole is provided on the bottom surface of the reaction tray, and a drip groove is slidably connected to the bottom surface at a position corresponding to the drainage hole. The drip groove has a notch, and a drainage pipe mouth is provided at the drainage hole. The drainage pipe mouth extends toward the drip groove and is provided at a far end from the notch.

8. A reaction method for immunohistochemical staining, characterized in that: include: When a target slide holder is loaded with a plurality of tissue slides, a target slide identifier of the target slide holder is obtained through a controller, and a target slide heating zone in a tissue sample incubation zone is determined based on the target slide identifier, wherein the tissue sample incubation zone includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating zones, a heater aligned with a slide groove on the slide holder is provided in the slide heating zone, and the reaction tray enters and exits the tissue sample incubation zone through a conveying device provided on both sides of the tissue sample incubation zone; The controller drives the robotic arm to place the slide holder carrying the target tissue slide in the target slide heating zone according to the target slide identifier, and controls the target heater on the target slide heating zone to heat according to a preset heating threshold.

9. The method according to claim 8, characterized in that The tissue sample incubation area further comprises a motor, which is electrically connected to the conveying device and is used to drive the conveying device according to the extension instruction or the entry instruction of the controller; querying the glass slide heating zone in the reaction tray which is in an idle state through the controller, and determining the target glass slide heating zone according to the idle state; The controller determines a conveying path for the robot arm to clamp the target glass slide holder, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target glass slide heating zone, the robot arm is driven according to the conveying path to clamp the glass slide holder and place it in the target glass slide heating zone. The conveying path is a route determined based on the placement position of the target glass slide heating zone and the clamping position of the target glass slide holder.

10. The method according to claim 1, characterized in that The tissue sample incubation area also includes a medicine refrigeration area and a medicine storage area arranged on the top layer of the reaction tray; the medicine refrigeration area is used to provide cold air to the reaction tray; the medicine storage area includes a plurality of medicine storage slots for storing medicine bottles; the medicine storage slots include a first medicine storage slot and a second medicine storage slot, the first medicine storage slots are arranged in a honeycomb shape, and the second medicine storage slots are arranged in parallel according to preset rows and preset columns; The controller determines the target medicine and the target dripping amount according to the target slide mark, determines the suction position of the dripping device for sucking and dripping the medicine according to the target medicine, and determines the dripping position of the dripping device in combination with the target slide heating zone; After the controller generates a driving route for the dripping device according to the suction position and the dripping position, the dripping device is driven to absorb medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating zone.

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