Intestinal repair composition and application thereof in detoxifying and beautifying

Abstract

The invention relates to the technical field of biological medicine, in particular to an intestinal repair composition and application thereof in detoxifying and beautifying, and the intestinal repair composition comprises 5 parts of probiotics, 28 parts of yeast beta-glucan, 9 parts of soybean peptide powder and 68 parts of inulin. The intestinal tract repairing composition has the beneficial effects that (1) the intestinal tract repairing composition can regulate intestinal tract flora, repair imbalance of the intestinal tract flora and promote intestinal tract movement, so that harmful substances such as intestinal tract stool and toxins are removed, symptoms such as constipation, diarrhea and dull yellow skin are effectively relieved, and the effects of relaxing bowel, expelling toxins, beautifying, whitening and fading spots are achieved; and (2) the intestinal tract repairing composition can improve the survival rate of the probiotics in the human body environment, can better promote the probiotics to colonize the intestinal tract, and achieves the purpose of quickly repairing the intestinal tract imbalance.

Description

Technical Field

[0001] The present invention relates to the field of biopharmaceutical technology, and in particular, to an intestinal repair composition and its application in detoxifying and beautifying the skin. Background Art

[0002] With the improvement of living standards, the increase in the proportion of meat consumption, high life pressure, and irregular diet of humans, the problems of the digestive tract have become increasingly prominent, and more than 90% of the population has gastrointestinal problems. The above-mentioned bad lifestyle is likely to cause intestinal flora imbalance and damage to internal organs, thereby leading to a decrease in the body's immune and detoxification abilities, directly affecting physical health.

[0003] The intestine is the largest digestive and detoxification organ of the human body. The nutrients required by the human body are all absorbed from the intestine, and more than 99% of the toxins accumulate in the intestinal folds. If defecation does not occur for more than two days, the thickness of the fecal residue can reach 5-7 cm; therefore, gastrointestinal diseases such as constipation and diarrhea often lead to the accumulation of toxins in the intestine and the loss of nutrients; when there are too many toxins in the body, it will lead to sub-healthy conditions such as cell damage, dull skin, fatigue, mental sluggishness, bad breath, skin itching, swelling, sallow complexion and emaciation, and further develop into cancer.

[0004] Currently, for intestinal diseases, generally, probiotics are supplemented to maintain intestinal balance and achieve the purpose of improving the intestine and enhancing immunity; however, the viable count of existing commercially available products is low and the colonization ability is poor, resulting in poor effects of the strains on improving intestinal flora imbalance and unsatisfactory effects on intestinal diseases. Summary of the Invention

[0005] In order to solve the above problems, the present invention firstly provides a first aspect of the invention, which provides an intestinal repair composition comprising probiotics, yeast β-glucan, soy peptide powder and inulin.

[0006] In some embodiments, the composition is in a ratio, by weight, of 1-10 parts of probiotics, 10-40 parts of yeast β-glucan, 1-20 parts of soy peptide powder and 50-80 parts of inulin.

[0007] In some embodiments, the composition is in a ratio, by weight, of 2-8 parts of probiotics, 15-30 parts of yeast β-glucan, 5-15 parts of soy peptide powder and 60-70 parts of inulin.

[0008] In some embodiments, the composition is in a ratio, by weight, of 5 parts of probiotics, 28 parts of yeast β-glucan, 9 parts of soy peptide powder and 68 parts of inulin.

[0009] In some embodiments, the probiotics are selected from one or more of Lactobacillus casei, Bifidobacterium lactis, Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus acidophilus.

[0010] In some embodiments, the probiotics comprise 5 - 20 parts of Lactobacillus casei, 5 - 20 parts of Bifidobacterium lactis, 1 - 10 parts of Lactobacillus plantarum, 1 - 20 parts of Streptococcus thermophilus, and 5 - 20 parts of Lactobacillus acidophilus.

[0011] In some embodiments, by weight, the proportion of the probiotics is 12 parts of Lactobacillus casei, 6 parts of Bifidobacterium lactis, 5 parts of Lactobacillus plantarum, 12 parts of Streptococcus thermophilus, and 15 parts of Lactobacillus acidophilus.

[0012] In some embodiments, by weight, the proportions of the probiotics are respectively 12 parts of Lactobacillus casei, 6 parts of Bifidobacterium lactis, 5 parts of Lactobacillus plantarum, 12 parts of Streptococcus thermophilus, and 15 parts of Lactobacillus acidophilus.

[0013] The present invention also provides the application of the above - mentioned intestinal repair composition in the preparation of foods or drugs with the functions of detoxifying and beautifying the skin.

[0014] Yeast β - glucan is a complex polysaccharide that can be absorbed by the intestine and activate macrophages to purify harmful substances; in addition, yeast β - glucan also has the functions of enhancing the body's immunity, removing toxins, and repairing cells.

[0015] Soybean peptide powder is a mixed peptide product composed of 2 - 6 amino acids with a molecular weight of 200 - 800 Dalton. Compared with the original protein, soybean peptide powder has good water solubility, water - holding capacity, and foaming property, and can be completely dissolved under any acid - base conditions of pH 2 - 10; in addition, soybean peptide powder is more easily absorbed by the human body, can enter the small intestine directly without gastrointestinal digestion, and is not affected by the physical condition; it contains a variety of bioactive peptides with various physiological activities, such as antioxidant, blood - pressure - lowering, cholesterol - lowering, blood - lipid - lowering, anti - fatigue, and immune - enhancing effects.

[0016] Inulin is a natural water - soluble dietary fiber that can hardly be hydrolyzed and digested by gastric acid and can be utilized by beneficial probiotics in the colon, thereby improving the intestinal environment; inulin can also enhance gastrointestinal peristalsis, improve gastrointestinal function, increase digestion and appetite, and improve the body's immunity.

[0017] Compared with the prior art, the present invention has at least the following beneficial effects:

[0018] (1) The composition of the present application can effectively improve the survival rate of probiotics in vivo, and thus can better promote the colonization of probiotics in the intestine, achieving the purpose of rapidly repairing intestinal imbalance; specifically, yeast β-glucan and soy peptide powder in the composition of the present application can also provide nutrients for probiotics and improve the repair ability of probiotics, and can improve the survival rate of probiotics in the human internal environment; in addition, inulin can also serve as a carrier for probiotics, soy peptide powder and yeast β-glucan, and can enrich each component into tiny groups, which is beneficial to resisting the damage caused by the human internal environment to probiotics and the loss of other active ingredients, and thus can improve the overall curative effect of the composition.

[0019] (2) Through the specific selection of probiotics, and with the synergistic effect among probiotics, the present application can better promote the colonization of each probiotic in the intestine, realize the rapid repair of the imbalance of the intestinal flora and promote intestinal peristalsis, achieving the purpose of rapidly and efficiently relieving intestinal diseases. Detailed implementation mode

[0020] To make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will be described in detail with reference to specific embodiments.

[0021] The soy peptide powder was purchased from Liaoning Tai'aitai Biotechnology Co., Ltd.;

[0022] Example 1

[0023] The intestinal repair composition comprises the following raw materials in parts by weight:

[0024] Probiotics 5 parts, yeast β-glucan 28 parts, soy peptide powder 9 parts and inulin 68 parts;

[0025] Each part of the probiotics comprises the following strains in parts by weight:

[0026] Lactobacillus casei 12 parts, Bifidobacterium lactis 6 parts, Lactobacillus plantarum 5 parts, Streptococcus thermophilus 12 parts and Lactobacillus acidophilus 15 parts; the viable count of all of them is 10 billion cfu / g.

[0027] The preparation method of the above-mentioned intestinal repair composition comprises the following steps:

[0028] Crush inulin and yeast β-glucan respectively, and then mix inulin, yeast β-glucan and soy peptide powder to obtain a mixture, wherein the particle size of the crushed inulin is 300-500 μm; the particle size of yeast β-glucan is not higher than 50 μm;

[0029] Sterilize the mixture at low temperature, and then add probiotics to the mixture and mix well to obtain the above-mentioned intestinal repair composition.

[0030] Example 2

[0031] The intestinal repair composition comprises raw materials in the following parts by weight:

[0032] 2 parts of probiotics, 20 parts of yeast β-glucan, 5 parts of soy peptide powder and 60 parts of inulin;

[0033] Among them, each part of probiotics comprises strains in the following parts by weight:

[0034] 10 parts of Lactobacillus casei, 10 parts of Bifidobacterium lactis, 2 parts of Lactobacillus plantarum, 15 parts of Streptococcus thermophilus and 10 parts of Lactobacillus acidophilus; the viable count of all of them is 10 billion cfu / g.

[0035] The preparation method of the above intestinal repair composition comprises the following steps:

[0036] Crush inulin and yeast β-glucan separately, and then mix inulin, yeast β-glucan and soy peptide powder to obtain a mixture. Among them, the particle size of the crushed inulin is 300-500 μm; the particle size of yeast β-glucan is not higher than 50 μm;

[0037] Sterilize the mixture at low temperature, and then add probiotics to the mixture and mix well to obtain the above intestinal repair composition.

[0038] Example 3

[0039] The intestinal repair composition comprises raw materials in the following parts by weight:

[0040] 4 parts of probiotics, 25 parts of yeast β-glucan, 10 parts of soy peptide powder and 65 parts of inulin;

[0041] Each part of the probiotics comprises strains in the following parts by weight:

[0042] 12 parts of Lactobacillus casei, 8 parts of Bifidobacterium lactis, 4 parts of Lactobacillus plantarum, 12 parts of Streptococcus thermophilus and 14 parts of Lactobacillus acidophilus; the viable count of all of them is 10 billion cfu / g.

[0043] The preparation method of the above intestinal repair composition comprises the following steps:

[0044] Crush inulin and yeast β-glucan separately, and then mix inulin, yeast β-glucan and soy peptide powder to obtain a mixture. Among them, the particle size of the crushed inulin is 300-500 μm; the particle size of yeast β-glucan is not higher than 50 μm;

[0045] Sterilize the mixture at low temperature, and then add probiotics to the mixture and mix well to obtain the above intestinal repair composition.

[0046] Comparative Example 1

[0047] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that inulin is omitted.

[0048] Comparative Example 2

[0049] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that yeast β-glucan is omitted.

[0050] Comparative Example 3

[0051] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that soy peptide powder is omitted.

[0052] Comparative Example 4

[0053] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that probiotics are omitted.

[0054] Comparative Example 5

[0055] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that inulin is replaced with fructooligosaccharide.

[0056] Comparative Example 6

[0057] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that inulin is replaced with polydextrose.

[0058] Control Example 7

[0059] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that yeast β-glucan is replaced with polydextrose.

[0060] Comparative Example 8

[0061] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that Lactobacillus casei is omitted.

[0062] Comparative Example 9

[0063] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that Bifidobacterium lactis is omitted.

[0064] Comparative Example 10

[0065] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that Lactobacillus plantarum is omitted.

[0066] Comparative Example 11

[0067] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that Streptococcus thermophilus is omitted.

[0068] Comparative Example 12

[0069] This example is an intestinal repair composition, and the difference between this intestinal repair composition and that of Example 1 is only that Lactobacillus acidophilus is omitted.

[0070] Effect Test 1: Acid resistance performance of probiotics in different compositions

[0071] Intestinal repair compositions of Examples 1-3 and Comparative Examples 1-12 were respectively prepared;

[0072] Artificial gastric acid was prepared, that is, a hydrochloric acid solution with a pH of 2.5 was prepared;

[0073] 3 g of the above different compositions were respectively dissolved in the hydrochloric acid solution with a pH of 2.5 and stored at anaerobic condition and 37 °C for 2 h, then cell counting was carried out, repeated 5 times, and the viable bacteria count was calculated. The calculation results are shown in Table 1:

[0074] Table 1 Results of viable bacteria count

[0075]

[0076]

[0077] As can be seen from Table 1:

[0078] The composition prepared in Example 1 of the present invention can significantly improve the survival rate of microorganisms (p<0.05); compared with Comparative Examples 1-3, it shows that inulin, yeast β-glucan or soy peptide powder cannot be omitted (p<0.001); compared with Comparative Examples 5-7, it shows that the effect of inulin is better than that of high molecular weight polysaccharides and oligosaccharides, and the effect of yeast β-glucan is better than that of polydextrose (p<0.001); compared with Comparative Examples 8-12, it shows that the effect of Example 1 is better than that after omitting a single strain (p<0.05); by specifically selecting each raw material in the present application, the technical solution of the present application has achieved the best effect.

[0079] Effect Test 2: Mouse constipation experiment

[0080] Establishment of constipation mouse model: After 1 week of adaptive feeding of mice, loperamide hydrochloride (5 mg / kg per single dose) was injected twice a day for 7 consecutive days. During this period, the mice drank water and ate normally. After one week, the feces of the mice were observed, and the mice with continuously reduced fecal particles, reduced number, reduced weight, and dry feces were selected as the successfully modeled mice.

[0081] Grouping: The successfully modeled mice were given drug treatment (experimental group). Meanwhile, a normal control group (normal mice were injected with the same volume of normal saline) and a model group (model mice) were set up, with 10 mice in each group. The normal control group and the model group were given normal saline by gavage, and the other experimental groups were given the intestinal repair compositions of Examples 1-3 and Comparative Examples 1-12 by gavage with water at a dose of 20 mg / day for 14 consecutive days. After administration on the 14th day, feces within 4 h were collected, and the fecal count, total fecal weight, and fecal water content were measured;

[0082] Fecal water content: Water content = (fecal mass before freeze-drying - fecal mass after freeze-drying) / fecal mass before freeze-drying × 100%.

[0083] The results are shown in Table 2:

[0084] Table 2 Results of preventing and treating constipation

[0085] Grouping Number of feces (pieces) Total weight of feces (g) Water content of feces (%) Blank control group 13.43±0.72 0.56±0.01 59.31±4.31 Model group 8.11±0.42 0.20±0.02 32.14±3.22 Example 1 13.21±0.31 0.51±0.01 57.03±5.21 Example 2 12.22±0.43 0.41±0.03 49.19±4.41 Example 3 12.57±0.31 0.44±0.02 51.24±5.31 Comparative example 1 10.23±0.42 0.33±0.01 39.54±5.31 Comparative example 2 10.91±0.64 0.39±0.01 43.08±4.21 Comparative example 3 11.21±0.51 0.40±0.03 44.19±3.31 Comparative example 4 8.51±0.51 0.22±0.01 33.34±2.29 Comparative example 5 11.99±0.42 0.41±0.01 47.14±2.91 Comparative example 6 12.11±0.51 0.41±0.01 48.01±3.48 Comparative example 7 12.01±0.72 0.41±0.03 47.19±5.31 Comparative example 8 12.69±0.36 0.42±0.02 50.24±2.39 Comparative example 9 12.29±0.41 0.40±0.01 48.14±4.22 Comparative example 10 12.41±0.44 0.41±0.01 49.01±3.41 Comparative example 11 12.61±0.41 0.41±0.03 50.29±3.75 Comparative example 12 13.01±0.54 0.39±0.02 48.24±4.31

[0086] Referring to the results in Table 2, it shows that the intestinal repair composition of Example 1 has the strongest ability to prevent and treat constipation (p < 0.05); comparing Example 1 with Comparative Examples 1-4, it shows that inulin, yeast β-glucan, soy peptide powder, or probiotics cannot be omitted (p < 0.001); comparing Example 1 with Comparative Examples 5-7, it shows that the effect of inulin is better than that of high oligosaccharides and low oligosaccharides, and the effect of yeast β-glucan is better than that of polydextrose (p < 0.001); comparing Example 1 with Comparative Examples 8-12, it shows that the effect of Example 1 is better than that after omitting a single bacterium (p < 0.05); through the specific selection of each raw material in this application being omitted, the technical solution of this application has achieved the best effect.

[0087] Effect test 3: Human test

[0088] A total of 150 volunteers aged 28-59 years with constipation and sallow skin symptoms were recruited. Each volunteer was randomly divided into 15 groups, with 10 people in each group. There were no statistical differences in age and symptom severity among the volunteers in each group; the 15 groups of people were respectively given the intestinal repair compositions of Examples 1-3 and Comparative Examples 1-12; the administration method was to take it with warm water, three times a day, 3 g each time; and it was continuously taken for 1 month;

[0089] After the administration ended, a follow-up visit was conducted, and each patient scored their satisfaction with the improvement of constipation and sallow skin (1-5 points), where 5 points represented very satisfied, 4 points represented satisfied, 3 points represented average, 2 points represented dissatisfied, and 1 point represented very dissatisfied; the average scores of each group were statistically analyzed, and the statistical results are shown in Table 3:

[0090] Table 3 Results of Satisfaction with the Improvement of Constipation and Sallow Skin

[0091]

[0092]

[0093] As can be seen from Table 3, the composition prepared in Example 1 of the present invention can significantly promote the excretion of feces and toxins, and effectively improve the symptoms of constipation and sallow skin. Comparing Example 1 with Comparative Examples 1-4, it shows that inulin, yeast β-glucan, soy peptide powder or probiotics cannot be omitted (p < 0.001). Comparing Example 1 with Comparative Examples 5-6, it shows that the effect of inulin is better than that of high oligosaccharides and low oligosaccharides (p < 0.001). Comparing Example 1 with Comparative Examples 8-12, it shows that the effect of Example 1 is better than that after omitting a single strain (p < 0.05). By omitting the specific selection of each raw material in this application, the technical solution of this application has achieved the best effect.

[0094] The above is the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A composition for intestinal repair, characterized in that: Contains probiotics, yeast beta-glucan, soy peptide powder and inulin.

2. The intestinal repair composition according to claim 1, characterized in that: The composition comprises, by weight, 1-10 parts of probiotics, 10-40 parts of yeast beta-glucan, 1-20 parts of soybean peptide powder and 50-80 parts of inulin.

3. The intestinal repair composition according to claim 2, characterized in that: The composition comprises 2-8 parts of probiotics, 15-30 parts of yeast beta-glucan, 5-15 parts of soybean peptide powder and 60-70 parts of inulin by weight.

4. The intestinal repair composition according to claim 3, characterized in that: The composition comprises 5 parts of probiotics, 28 parts of yeast beta-glucan, 9 parts of soybean peptide powder and 68 parts of inulin by weight.

5. The intestinal repair composition according to claim 1, characterized in that: The probiotics are selected from one or more of Lactobacillus casei, Bifidobacterium lactis, Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus acidophilus.

6. The intestinal repair composition according to claim 5, characterized in that: The probiotics are in the following proportions by weight: 5-20 parts of Lactobacillus casei, 5-20 parts of Bifidobacterium lactis, 1-10 parts of Lactobacillus plantarum, 1-20 parts of Streptococcus thermophilus and 5-20 parts of Lactobacillus acidophilus.

7. The intestinal repair composition according to claim 6, characterized in that: The probiotics are calculated by weight in the following proportions: 12 parts of Lactobacillus casei, 6 parts of Bifidobacterium lactis, 5 parts of Lactobacillus plantarum, 12 parts of Streptococcus thermophilus and 15 parts of Lactobacillus acidophilus.

8. The intestinal repair composition according to claim 7, characterized in that: The probiotics are calculated by weight in the following proportions: 12 parts of Lactobacillus casei, 6 parts of Bifidobacterium lactis, 5 parts of Lactobacillus plantarum, 12 parts of Streptococcus thermophilus and 15 parts of Lactobacillus acidophilus.

9. Use of the intestinal repair composition according to any one of claims 1 to 8 in the preparation of food or medicine with detoxification and beauty effects.

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