Synergistic and allergy-reducing idebenone anti-aging and skin-brightening co-delivery carrier and application thereof

Abstract

The invention relates to the field of cosmetics, in particular to an idebenone anti-aging and skin-brightening co-delivery carrier capable of improving effect and reducing sensitivity and application thereof. The idebenone, the wolfberry extract and the peony root extract are reasonably matched, so that multi-target co-delivery and multi-mechanism anti-aging can be realized. According to the anti-aging skin-brightening co-delivery carrier of idebenone, anti-aging functional components are co-packaged and delivered by using a nano-carrier technology, and the obtained anti-aging skin-brightening co-delivery carrier of idebenone has good stability and water dispersibility, increases the solubility of the functional components, and also improves the irritation of the functional components. The carrier can promote functional components to efficiently penetrate through a skin barrier and rapidly reach a dermis layer deep in skin, and effectively stimulate fibroblast renewal, so that synthesis of collagen and elastin is promoted, skin elasticity is enhanced, and skin renewal is promoted; the composition can be enriched in target tissues at high concentration, detained for a long time, slowly released and released, and effectively taken by target cells, so that the bioavailability of functional components is improved, the anti-aging effect is enhanced, and the action time is prolonged.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and particularly to a co-delivery carrier of idebenone for anti-aging and skin brightening with enhanced efficacy and reduced sensitivity, and its application. Background Art

[0002] As is well known, external factors such as ultraviolet rays and pollution, as well as internal factors such as hormones and inflammation levels, act on the skin together, causing inflammation and excessive free radicals (ROS) in the skin through different mechanisms and pathways, resulting in damage to DNA, proteins, and lipids, and causing cell aging. Skin aging starts from the dermis layer, and the aging of the stratum corneum and sebum membrane is its surface phenomenon. Generally speaking, the focus of anti-aging skin care is on three aspects: tightening the skin, removing wrinkles, and delaying aging. Anti-aging skin care products need to reach the dermis layer and be absorbed by the skin to be effective, and anti-aging skin care needs to improve the skin condition of the dermis layer. However, many anti-aging active ingredients are difficult to penetrate the stratum corneum and enter the dermis layer, and it is difficult to accumulate to an effective concentration range at the target site, greatly reducing the actual anti-aging effect of the skin.

[0003] There are a wide variety of anti-aging products on the current market, but most of the active ingredients with anti-aging functions have poor stability and are easily oxidized, denatured, or inactivated when directly applied to cosmetic products. In addition, the formula design of anti-aging products is not perfect enough, the action mechanism is single, and there is a certain degree of irritation. For example, idebenone as an anti-aging ingredient has strong irritation to the skin and eyes and poor chemical stability, which is not conducive to practical application.

[0004] From the ingredients of anti-aging products on the market, it can be seen that at present, most products exist in cosmetics in a free state or only use anti-aging raw materials with a single ingredient, and mainly achieve corresponding effects by scavenging free radicals (ROS), inhibiting the production of inflammatory factors, and inhibiting the synthesis of melanin by melanocytes. In addition, when active substances are used in cosmetics, most of them are added to products in a free state, with low bioavailability and strong irritation, so it is difficult to achieve good anti-aging effects and allergic reactions are likely to occur. Therefore, many anti-aging products cannot achieve satisfactory effects for consumers.

[0005] Generally speaking, the current anti-aging products have limited action targets, a single action mechanism, and are difficult to penetrate the skin and accumulate to an effective concentration range at the target site, ultimately resulting in an unsatisfactory anti-aging effect. Summary of the Invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a co-delivery carrier of idebenone for anti-aging and skin brightening, which has excellent anti-aging and antioxidant effects and can be applied to cosmetics.

[0007] To achieve the above purpose, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a co-delivery carrier for idebenone anti-aging and skin brightening. The co-delivery carrier for idebenone anti-aging and skin brightening comprises a nanocarrier and an anti-aging composition encapsulated in the nanocarrier; the anti-aging composition comprises components in the following parts by weight: 1-5 parts of idebenone, 1-5 parts of wolfberry extract, and 1-5 parts of peony root extract; the nanocarrier comprises at least two of the following components: phospholipid, emulsifier, co-emulsifier, polyol, cholesterol, and water.

[0008] Idebenone is a lipophilic compound, structurally similar to coenzyme Q10. As a highly efficient antioxidant, it has the effects of scavenging free radicals, inhibiting lipid peroxidation, inhibiting inflammation, inhibiting DNA damage, photoprotection, reducing pigmentation, etc. Wolfberry extract is rich in antioxidants such as wolfberry polysaccharides, vitamin C, vitamin E, and flavonoids. These components can neutralize free radicals, slow down skin aging, brighten skin tone, fade age spots, and strengthen the skin barrier. The fatty acids and other nutrients it contains help enhance the skin barrier function and improve skin resistance, etc. The flavonoids in peony root extract have strong antioxidant ability, can neutralize free radicals, inhibit the production of melanin, slow down the process of skin aging, and maintain skin elasticity. Single active ingredients have the disadvantages of strong irritation and instability. Considering various anti-aging mechanisms comprehensively, the present invention targets skin cells and combines three active ingredients, idebenone, wolfberry extract, and peony root extract, through ways such as scavenging free radicals, inhibiting the generation path of free radicals, reducing the activity of melanin-producing related enzymes, promoting the synthesis of collagen and elastin, and reducing oxidative damage, to form a multi-functional anti-aging nano-composition, which can have synergistic effects. A co-delivery carrier for anti-aging and skin brightening with idebenone is obtained by encapsulating the three scientifically combined active ingredients with a nano-carrier. Based on the structure and characteristics of the nano-carrier, the composition obtained by nano-encapsulation is easily soluble in water, convenient to use, has good stability and water dispersibility, increases the solubility of the active ingredients, and at the same time improves the irritation of the active ingredients, so that the active ingredients can reach a sufficient concentration in the product to exert corresponding functional effects. While effectively improving the solubility of each active ingredient, it provides a stable storage space for them, avoiding unnecessary degradation or inactivation of the active ingredients before storage and use, which is beneficial to increasing the concentration of active substances in anti-aging products. More importantly, the present invention makes full use of the advantages of the nano-carrier such as percutaneous penetration characteristics, biocompatibility, controlled release and sustained release characteristics, etc. The co-delivery carrier for anti-aging and skin brightening with idebenone can promote the three above-mentioned active ingredients to efficiently penetrate through the skin barrier and quickly reach the dermis deep in the skin, effectively stimulating the renewal of fibroblasts, thereby promoting the synthesis of collagen and elastin, enhancing skin elasticity, and promoting skin renewal. Cosmetics added with the co-delivery carrier for anti-aging and skin brightening with idebenone have good skin permeability and retention performance after use, can be highly concentrated in the target tissue, stay for a long time, have controlled release and sustained release, are effectively taken up by target cells, improve the bioavailability of the active ingredients, enhance the anti-aging effect, and extend the action time.

[0009] Preferably, the average particle size of the co-delivery carrier prepared by the present invention is between 16.33 - 18.16 nm, the particle size is relatively concentrated, and it has good permeability.

[0010] Preferably, the weight ratio of the nano-carrier to the anti-aging composition is nano-carrier: anti-aging composition = (63.8 - 164): 9.

[0011] Preferably, the anti-aging composition comprises the following components in parts by weight: idebenone 3 parts, wolfberry extract 3 parts, and peony root extract 3 parts.

[0012] Preferably, the nano-carrier comprises the following components in parts by weight: phospholipid 1 - 5 parts, emulsifier 10 - 50 parts, co-emulsifier 1 - 10 parts, polyol 10 - 50 parts, cholesterol 0.1 - 1 part, and water 20 - 50 parts.

[0013] Preferably, the phospholipid comprises at least one of hydrogenated lecithin, lecithin, and soy lecithin.

[0014] Preferably, the emulsifier comprises at least one of polyoxyethylene castor oil emulsifiers, polyoxyethylene hydrogenated castor oil emulsifiers, polyglycerol emulsifiers, poloxamer, and cocoyl glucoside.

[0015] Preferably, the co-emulsifier comprises at least one of laureth-23, diethylene glycol monoethyl ether, PPG-26-buteth-26, trideceth-12, 1,3-propanediol, 1,2-pentanediol, 1,2-hexanediol, dipropylene glycol, glycerol, and butanediol.

[0016] Preferably, the polyol comprises at least one of glycerol, ethanol, propylene glycol, butanediol, 1,3-propanediol, 1,2-pentanediol, 1,2-hexanediol, octyldodecanol, dipropylene glycol, isopropanol, ethoxydiglycol, polyethylene glycol-200, and PPG-10 sorbitol.

[0017] Preferably, the phospholipid is lecithin.

[0018] Preferably, the emulsifier comprises PEG-40 hydrogenated castor oil, polyglycerol-10 laurate, and polysorbate-80.

[0019] More preferably, in the emulsifier, the weight ratio of PEG-40 hydrogenated castor oil, polyglycerol-10 laurate, and polysorbate-80 is PEG-40 hydrogenated castor oil:polyglycerol-10 laurate:polysorbate-80 = (3 - 20):(5 - 20):(2 - 10).

[0020] Preferably, the co-emulsifier is PPG-26-buteth-26.

[0021] Preferably, the polyol comprises octyldodecanol, ethoxydiglycol, and 1,2-pentanediol.

[0022] More preferably, in the polyol, the weight ratio of octyldodecanol, ethoxydiglycol, and 1,2-pentanediol is octyldodecanol:ethoxydiglycol:1,2-pentanediol = (3 - 15):(2 - 17):(5 - 18).

[0023] Preferably, the co-delivery carrier of idebenone for anti-aging and skin brightening comprises the following components in parts by weight: 3 parts of idebenone, 3 parts of wolfberry fruit extract, 3 parts of peony root extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0024] In a second aspect, the present invention provides a method for preparing the co-delivery carrier of idebenone for anti-aging and skin brightening, comprising the following steps: (1) Mix idebenone, polyol, cholesterol and phospholipid, and stir evenly to obtain a mixed solution A; (2) Add the emulsifier and co-emulsifier to the mixed solution A obtained in step (1), and stir evenly to obtain a mixed solution B; (3) Mix the wolfberry fruit extract, peony root extract and water, and stir evenly to obtain a mixed solution C; (4) Add the mixed solution C obtained in step (3) to the mixed solution B obtained in step (2), mix well, stir, and homogenize to obtain the co-delivery carrier of idebenone for anti-aging and skin brightening.

[0025] Preferably, in steps (1) and (2), the stirring conditions are: stirring at 45°C - 65°C and 150 - 200 rpm for 15 min.

[0026] Preferably, in step (3), the stirring conditions are: stirring at 150 - 200 rpm for 10 min.

[0027] Preferably, in step (4), the stirring conditions are: stirring at 200 - 270 rpm for 20 min.

[0028] Preferably, in step (4), the homogenization conditions are 800 - 1000 bar.

[0029] In a third aspect, the present invention provides the application of the co-delivery carrier of idebenone for anti-aging and skin brightening in anti-aging cosmetics.

[0030] Preferably, the dosage forms of the anti-aging cosmetics include lotion, milk, and cream.

[0031] In a fourth aspect, the present invention provides an anti-aging cosmetic, comprising the co-delivery carrier of idebenone for anti-aging and skin brightening.

[0032] Preferably, the addition amount of the co-delivery carrier of idebenone for anti-aging and skin brightening in the cosmetic is 0.1 - 30%.

[0033] More preferably, the addition amount of the co-delivery carrier of idebenone for anti-aging and skin brightening in the cosmetic is 5%.

[0034] The beneficial effects of the present invention are as follows: The present invention comprehensively considers various anti-aging mechanisms, targets skin cells, and forms a multi-functional anti-aging nano-composition by reasonably combining three active ingredients, idebenone, wolfberry extract, and peony root extract, from the aspects of scavenging free radicals, inhibiting the generation pathway of free radicals, reducing the activity of melanin-producing enzymes, promoting the synthesis of collagen and elastin, and reducing oxidative damage, etc., which can synergistically enhance the effects. Using nanocarrier technology, the anti-aging active ingredients with multiple mechanisms are co-encapsulated and delivered to obtain an idebenone anti-aging and skin brightening co-delivery carrier. Based on the structure and characteristics of the nanocarrier, the composition obtained by nano-encapsulation is easily soluble in water, convenient to use, has good stability and water dispersibility, increases the solubility of the active ingredients, and at the same time improves the irritation of the active ingredients, so that the active ingredients can reach a sufficient concentration in the product to exert corresponding functional effects. While effectively improving the solubility of each active ingredient, it provides a stable storage space for them, avoiding unnecessary degradation or inactivation of the active ingredients before storage and use, which is beneficial to increasing the concentration of active substances in anti-aging products. More importantly, the present invention makes full use of the advantages of the percutaneous penetration characteristics, biocompatibility, controlled release and sustained release characteristics of the nanocarrier. The idebenone anti-aging and skin brightening co-delivery carrier can promote the efficient penetration of the above three active ingredients through the skin barrier and quickly reach the dermis deep in the skin, effectively stimulating the renewal of fibroblasts, thereby promoting the synthesis of collagen and elastin, enhancing skin elasticity, and promoting skin renewal. The cosmetics added with the idebenone anti-aging and skin brightening co-delivery carrier have good skin permeability and retention performance, can be highly concentrated and retained in the target tissue for a long time, with controlled release and sustained release, and can be effectively taken up by target cells, improving the bioavailability of the active ingredients, enhancing the anti-aging effect, and prolonging the action time. Description of the Drawings

[0035] Figure 1 Results of the irritation evaluation of the idebenone anti-aging and skin brightening co-delivery carrier and the free composition.

[0036] Figure 2 Results of the determination of the skin cumulative permeation amount and skin retention amount of the idebenone anti-aging and skin brightening co-delivery carrier and the free composition.

[0037] Figure 3 Results of the comparison of the HSF cell survival rates of the idebenone anti-aging and skin brightening co-delivery carrier and the free composition. Detailed Embodiments

[0038] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0039] The raw materials used in the present invention: wolfberry extract and peony root extract are both purchased from Xi'an Shilin Biotechnology Co., Ltd.

[0040] Unless otherwise specified, the raw materials used in the present invention are all conventional commercially available products.

[0041] Example 1 An embodiment of the co-delivery carrier for idebenone anti-aging and skin brightening according to the present invention; it comprises the following components in parts by weight: 3 parts of idebenone, 3 parts of wolfberry extract, 3 parts of peony root extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0042] The phospholipid is lecithin; the emulsifier comprises 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is 1 part of PPG-26-butanol polyether; the polyol comprises 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0043] The preparation method of the co-delivery carrier for idebenone anti-aging and skin brightening according to the present invention comprises the following steps: (1) Mix idebenone, polyol, cholesterol and phospholipid, and stir at 45°C - 65°C and 150 - 200 rpm for 15 min to form a homogeneous and clear mixture A; (2) Add the emulsifier and co-emulsifier to mixture A, and stir at 45°C - 65°C and 150 - 200 rpm for 15 min to form a homogeneous and clear mixture B; (3) Mix the wolfberry extract, peony root extract and water, and stir at 150 - 200 rpm for 10 min to form a homogeneous and clear mixture C; (4) Add mixture C to mixture B and mix well. After stirring at 200 - 270 rpm for 20 min, homogenize the mixed liquid twice at 800 - 1000 bar in a high-pressure homogenizer to obtain the co-delivery carrier for idebenone anti-aging and skin brightening.

[0044] Example 2 An embodiment of the co-delivery carrier for idebenone anti-aging and skin brightening according to the present invention; it comprises the following components in parts by weight: 5 parts of idebenone, 1 part of wolfberry extract, 3 parts of peony root extract, 2 parts of phospholipid, 10 parts of emulsifier, 5 parts of co-emulsifier, 26 parts of polyol, 0.8 part of cholesterol, and 20 parts of water.

[0045] The phospholipid is lecithin; the emulsifier comprises 3 parts of PEG-40 hydrogenated castor oil, 5 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is 5 parts of PPG-26-butanol polyether; the polyol comprises 11 parts of octyldodecanol, 3 parts of ethoxydiglycol, and 12 parts of 1,2-pentanediol.

[0046] The preparation method of the idebenone anti-aging and skin brightening co-delivery carrier is the same as that in Example 1.

[0047] Example 3 An embodiment of the idebenone anti-aging and skin brightening co-delivery carrier of the present invention; it comprises the following components in parts by weight: 1 part of idebenone, 3 parts of wolfberry extract, 5 parts of peony root extract, 5 parts of phospholipid, 35 parts of emulsifier, 6 parts of co-emulsifier, 35 parts of polyol, 0.1 part of cholesterol, and 40 parts of water.

[0048] The phospholipid is lecithin; the emulsifier comprises 20 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 5 parts of polyglyceryl-10 laurate; the co-emulsifier is PPG-26-butanol polyether 6 parts; the polyol comprises 15 parts of octyldodecanol, 8 parts of ethoxydiglycol, and 12 parts of 1,2-pentanediol.

[0049] The preparation method of the idebenone anti-aging and skin brightening co-delivery carrier is the same as that in Example 1.

[0050] Example 4 An embodiment of the idebenone anti-aging and skin brightening co-delivery carrier of the present invention; it comprises the following components in parts by weight: 3 parts of idebenone, 5 parts of wolfberry extract, 1 part of peony root extract, 3 parts of phospholipid, 50 parts of emulsifier, 10 parts of co-emulsifier, 50 parts of polyol, 1 part of cholesterol, and 50 parts of water.

[0051] The phospholipid is lecithin; the emulsifier comprises 20 parts of PEG-40 hydrogenated castor oil, 20 parts of polysorbate-80, and 10 parts of polyglyceryl-10 laurate; the co-emulsifier is PPG-26-butanol polyether 10 parts; the polyol comprises 15 parts of octyldodecanol, 17 parts of ethoxydiglycol, and 18 parts of 1,2-pentanediol.

[0052] The preparation method of the idebenone anti-aging and skin brightening co-delivery carrier is the same as that in Example 1.

[0053] Comparative Example 1 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier of the present invention; it comprises the following components in parts by weight: 9 parts of idebenone, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0054] The phospholipid is lecithin; the emulsifier comprises 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is PPG-26-butanol polyether 1 part; the polyol comprises 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0055] The preparation method of the idebenone anti-aging and skin brightening co-delivery carrier is only different from that of Example 1 in that: in this comparative example, wolfberry extract and peony root extract are not added.

[0056] Comparative Example 2 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier described in the present invention; this comparative example is an anti-aging and skin brightening co-delivery carrier, comprising the following components in parts by weight: 9 parts of wolfberry extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0057] The phospholipid is lecithin; the emulsifier includes 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is PPG-26-butanol polyether 1 part; the polyol includes 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0058] The preparation method of the anti-aging and skin brightening co-delivery carrier is only different from that of Example 1 in that: in this comparative example, idebenone and peony root extract are not added.

[0059] Comparative Example 3 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier described in the present invention; this comparative example is an anti-aging and skin brightening co-delivery carrier, comprising the following components in parts by weight: 9 parts of peony root extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0060] The phospholipid is lecithin; the emulsifier includes 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is PPG-26-butanol polyether 1 part; the polyol includes 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0061] The preparation method of the anti-aging and skin brightening co-delivery carrier is only different from that of Example 1 in that: in this comparative example, idebenone and wolfberry extract are not added.

[0062] Comparative Example 4 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier described in the present invention; comprising the following components in parts by weight: 3 parts of idebenone, 3 parts of peony root extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0063] The phospholipid is lecithin; the emulsifier includes 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is 1 part of PPG-26-buteth-16; the polyol includes 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0064] The preparation method of the idebenone anti-aging and skin brightening co-delivery carrier is only different from that of Example 1 in that: this comparative example does not add wolfberry extract.

[0065] Comparative Example 5 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier of the present invention; this comparative example is an anti-aging and skin brightening co-delivery carrier, including the following components in parts by weight: 3 parts of wolfberry extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0066] The phospholipid is lecithin; the emulsifier includes 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is 1 part of PPG-26-buteth-16; the polyol includes 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0067] The preparation method of the anti-aging and skin brightening co-delivery carrier is only different from that of Example 1 in that: this comparative example does not add idebenone and peony root extract.

[0068] Comparative Example 6 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier of the present invention; this comparative example is an anti-aging and skin brightening co-delivery carrier, including the following components in parts by weight: 3 parts of idebenone, 3 parts of peony root extract, 3 parts of grape seed extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0069] The phospholipid is lecithin; the emulsifier includes 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is 1 part of PPG-26-buteth-16; the polyol includes 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0070] The preparation method of the anti-aging and skin brightening co-delivery carrier is only different from that of Example 1 in that: this comparative example replaces the wolfberry extract with grape seed extract.

[0071] Comparative Example 7 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier of the present invention; this comparative example is an anti-aging and skin brightening co-delivery carrier, comprising the following components in parts by weight: 6 parts of idebenone, 6 parts of peony root extract, 6 parts of wolfberry fruit extract, 1 part of phospholipid, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

[0072] The phospholipid is lecithin; the emulsifier includes 15 parts of PEG-40 hydrogenated castor oil, 10 parts of polysorbate-80, and 2 parts of polyglyceryl-10 laurate; the co-emulsifier is 1 part of PPG-26-butanol polyether; the polyol includes 3 parts of octyldodecanol, 2 parts of ethoxydiglycol, and 5 parts of 1,2-pentanediol.

[0073] The preparation method of the anti-aging and skin brightening co-delivery carrier is the same as that of Example 1.

[0074] Comparative Example 8 A comparative example of the idebenone anti-aging and skin brightening co-delivery carrier of the present invention; this comparative example is a free composition.

[0075] The free composition includes the following components in parts by weight: 3 parts of idebenone, 3 parts of wolfberry fruit extract, 3 parts of peony root extract, 9.1 parts of dimethyl sulfoxide (DMSO), and 81.9 parts of water.

[0076] The preparation method of the free composition includes the following steps: Mix idebenone, wolfberry fruit extract, peony root extract, dimethyl sulfoxide, and water (1:9) evenly to obtain the free composition.

[0077] Application Example 1 An application example of the cosmetic of the present invention; the cosmetic is a serum.

[0078] The serum includes the following components in mass percentage (based on the total mass of 100%): 5% of the idebenone anti-aging and skin brightening co-delivery carrier of Example 1, 5% of dipropylene glycol, 10% of glycerin, 15% of propylene glycol, 0.15% of carbopol 941, 0.15% of TEA (triethanolamine), 0.5% of DM928 (1,2-hexanediol / caprylohydroxamic acid / ethylhexylglycerin / propylene glycol), and the balance is water.

[0079] The preparation method of the serum includes the following steps: (1) Add dipropylene glycol, glycerin, propylene glycol, and carbopol 941 to water, and stir and dissolve at 35-45 °C and 400-500 rpm until clear, uniform, and free of agglomeration to obtain a mixed solution A; (2) Stir the mixed solution A obtained in step (1) at 400 - 5000 rpm, and simultaneously add triethanolamine (TEA) and DM928 (a mixture of 1,2 - hexanediol, octanoyl hydroxamic acid, ethylhexyl glycerin, and propylene glycol), then slowly stir at 200 - 300 rpm for 15 min until the liquid is homogeneous to obtain a mixed solution B; (3) Add idebenone anti - aging and brightening co - delivery carrier to the mixed solution B obtained in step (2) and mix evenly to obtain the essence.

[0080] Application Comparative Example 1 An application comparative example of the cosmetic of the present invention; the cosmetic is an essence.

[0081] The difference between the essence and that in Application Example 1 is only that: the idebenone anti - aging and brightening co - delivery carrier in Example 1 of Application Example 1 is replaced with the idebenone anti - aging and brightening co - delivery carrier of Comparative Example 1.

[0082] The preparation method of the essence is the same as that in Application Example 1.

[0083] Application Comparative Example 2 An application comparative example of the cosmetic of the present invention; the cosmetic is an essence.

[0084] The difference between the essence and that in Application Example 1 is only that: the idebenone anti - aging and brightening co - delivery carrier in Example 1 of Application Example 1 is replaced with the anti - aging and brightening co - delivery carrier of Comparative Example 2.

[0085] The preparation method of the essence is the same as that in Application Example 1.

[0086] Application Comparative Example 3 An application comparative example of the cosmetic of the present invention; the cosmetic is an essence.

[0087] The difference between the essence and that in Application Example 1 is only that: the idebenone anti - aging and brightening co - delivery carrier in Example 1 of Application Example 1 is replaced with the anti - aging and brightening co - delivery carrier of Comparative Example 3.

[0088] The preparation method of the essence is the same as that in Application Example 1.

[0089] Application Comparative Example 4 An application comparative example of the cosmetic of the present invention; the cosmetic is an essence.

[0090] The difference between the essence and that in Application Example 1 is only that: the idebenone anti - aging and brightening co - delivery carrier in Example 1 of Application Example 1 is replaced with the anti - aging and brightening co - delivery carrier of Comparative Example 4.

[0091] The preparation method of the essence is the same as that in Application Example 1.

[0092] Application Comparative Example 5 A kind of application comparative example of the cosmetic of the present invention; the cosmetic is essence.

[0093] The difference between the essence and Application Example 1 is only that: the idebenone anti-aging and skin brightening co-delivery carrier in Example 1 of Application Example 1 is replaced with the anti-aging and skin brightening co-delivery carrier of Comparative Example 5.

[0094] The preparation method of the essence is the same as that of Application Example 1.

[0095] Application Comparative Example 6 A kind of application comparative example of the cosmetic of the present invention; the cosmetic is essence.

[0096] The difference between the essence and Application Example 1 is only that: the idebenone anti-aging and skin brightening co-delivery carrier in Example 1 of Application Example 1 is replaced with the anti-aging and skin brightening co-delivery carrier of Comparative Example 6.

[0097] The preparation method of the essence is the same as that of Application Example 1.

[0098] Application Comparative Example 7 A kind of application comparative example of the cosmetic of the present invention; the cosmetic is essence.

[0099] The difference between the essence and Application Example 1 is only that: the idebenone anti-aging and skin brightening co-delivery carrier in Example 1 of Application Example 1 is replaced with the anti-aging and skin brightening co-delivery carrier of Comparative Example 7.

[0100] The preparation method of the essence is the same as that of Application Example 1.

[0101] Application Comparative Example 8 A kind of application comparative example of the cosmetic of the present invention; the cosmetic is essence.

[0102] The difference between the essence and Application Example 1 is only that: the idebenone anti-aging and skin brightening co-delivery carrier in Example 1 of Application Example 1 is replaced with the free composition of Comparative Example 8.

[0103] The preparation method of the essence is the same as that of Application Example 1.

[0104] Test Example 1: Characterization test and stability test Test samples: Idebenone anti-aging and skin brightening co-delivery carriers / anti-aging and skin brightening co-delivery carriers of Examples 1-4 and Comparative Examples 1-7.

[0105] 1. Use an instrument to detect the particle size and PDI of the test samples.

[0106] The results showed that the average particle sizes of the idebenone anti-aging and skin brightening co-delivery carriers / carriers of Examples 1-4 and Comparative Examples 1-7 were within 100 nm, and the PDI was 0.05-0.2. Among them, the average particle size of the idebenone anti-aging and skin brightening co-delivery carrier of Example 1 was 16.75 nm, and the PDI was 0.076; the average particle size of the idebenone anti-aging and skin brightening co-delivery carrier of Example 2 was 16.33 nm, and the PDI was 0.095; the average particle size of the idebenone anti-aging and skin brightening co-delivery carrier of Example 3 was 17.13 nm, and the PDI was 0.065; the average particle size of the idebenone anti-aging and skin brightening co-delivery carrier of Example 4 was 18.16 nm, and the PDI was 0.072.

[0107] The average particle size of the carrier of Comparative Example 1 was 18.75 nm, and the PDI was 0.102; the average particle size of the carrier of Comparative Example 2 was 19.12 nm, and the PDI was 0.092; the average particle size of the carrier of Comparative Example 3 was 17.93 nm, and the PDI was 0.086; the average particle size of the carrier of Comparative Example 4 was 20.01 nm, and the PDI was 0.116; the average particle size of the carrier of Comparative Example 5 was 19.65 nm, and the PDI was 0.106; the average particle size of the carrier of Comparative Example 6 was 17.62 nm, and the PDI was 0.096; the average particle size of the carrier of Comparative Example 7 was 18.82 nm, and the PDI was 0.083.

[0108] It can be seen that the range of the average particle size of the carriers prepared in the comparative examples was between 17.62-20.01 nm, while the average particle size of the co-delivery carriers prepared in the examples of the present invention was between 16.33-18.16 nm, the particle size was more concentrated, and the average particle size was smaller, having better permeability.

[0109] 2. Place the idebenone anti-aging and skin brightening co-delivery carriers / carriers of Examples 1-4 and Comparative Examples 1-7 in a sealed container, and place them at -20°C, room temperature, 4°C, and 45°C for 3 months respectively, and observe the changes in their stability.

[0110] The results showed that no layering or precipitation occurred in the test samples, and the particle size did not change significantly, indicating that the stability of the anti-aging and skin brightening co-delivery carriers prepared in the present invention was good.

[0111] Test Example 2: Evaluation of irritation to chicken embryo chorioallantoic membrane Test samples: The idebenone anti-aging and skin brightening co-delivery carriers of Examples 1-4, the free composition of Comparative Example 8.

[0112] Test method: Dilute the test sample by 10 times, then take 0.2 mL and drop it on the surface of the chorioallantoic membrane. Observe the changes in CAM blood vessels within 5 min and record the initial time when hyperemia, bleeding, and blood coagulation occur in the CAM blood vessels, and calculate the irritation score IS.

[0113] Calculate the irritation score (irritation score, IS) according to the following formula: IS = [(301 - secH) × 5 + (301 - secL) × 7 + (301 - secC) × 9] / 300 In the above formula, secH represents the initial time of hyperemia (s); secL represents the initial time of bleeding (s); secC represents the initial time of blood coagulation (s).

[0114] The results are as Figure 1 shown. The irritation evaluation results of the co - delivery carrier of idebenone for anti - aging and brightening skin in Examples 1 - 4 are similar. When the co - delivery carrier of idebenone for anti - aging and brightening skin after being diluted by 10 times contacts the chorioallantoic membrane of chicken embryos for 300 s, there is no bleeding, no blood vessel lysis, and no blood coagulation in the capillaries, and the reaction score is 0.07, indicating that the co - delivery carrier of idebenone for anti - aging and brightening skin in Examples 1 - 4 after being diluted by 10 times has good safety and no irritation. After the free composition (Comparative Example 8) diluted by 10 times contacts the chorioallantoic membrane of chicken embryos for 300 s, there is bleeding in the capillaries, and the reaction score is 4.89, showing irritation. This indicates that the active ingredient can reduce the irritation of the active substance itself after being encapsulated by the co - delivery carrier of idebenone for anti - aging and brightening skin prepared by the present invention.

[0115] Test Example 3: Patch test Test sample: The co - delivery carrier of idebenone for anti - aging and brightening skin in Examples 1 - 4, formulated into a 10% cream.

[0116] Select 30 subjects, and use a patch tester to apply the 10% cream of the co - delivery carrier of idebenone for anti - aging and brightening skin and the blank cream (without the co - delivery carrier of idebenone for anti - aging and brightening skin) on the flexor side of the forearm of the subjects for 24 h. After removing the patch tester, wait for 30 min, and observe the skin reaction after the indentation disappears. Observe the skin reaction again 24 h and 48 h after removing the patch tester.

[0117] The results show that none of the 30 subjects showed symptoms such as faint erythema, erythema, edematous erythema, significant swelling, infiltration, or papules and papules or blisters, indicating that the co - delivery carrier of idebenone for anti - aging and brightening skin prepared by the present invention has no irritation to human skin.

[0118] Test Example 4: Determination of skin cumulative permeation amount and skin retention amount Test sample: The essence prepared in Example 1 and Comparative Example 8.

[0119] The Franz diffusion cell method was used for the transdermal experiment of ex vivo porcine skin. 0.5 g of each test sample was placed in the supply chamber, and PBS (pH 7.4) was used as the receiving solution. HPLC analysis was used to calculate the cumulative amount of the active ingredient permeated per unit area at different times. After 24 h, the supernatant of the skin homogenate was taken for HPLC analysis to calculate the amount of the active ingredient retained per unit area of the skin. The cumulative skin permeation amount of the active ingredient at different sampling times was calculated according to the following formula: In the above formula, Q n is the cumulative drug permeation amount, C n is the drug concentration measured at the nth time, C i is the drug concentration measured at the ith point, V 0 is the volume of the diffusion cell, that is, the amount of the release medium added, V i is the amount of each sampling. The cumulative permeation amount per unit area Q = Q n / S, where S is the area of the diffusion cell, 2.27 cm 2 .

[0120] The results are as Figure 2 shown. The cumulative amount of idebenone permeated per unit area of the skin in 24 h in the essence prepared in Application Example 1 and Application Comparative Example 8 was 19.45 μg / cm 2 and 6.27 μg / cm 2 respectively, and the skin retention amounts were 56.19 μg / cm 2 and 10.48 μg / cm 2 respectively.

[0121] Compared with Application Comparative Example 8, the cumulative amount of idebenone permeated per unit area of the skin in 24 h in the idebenone anti-aging and skin brightening co-delivery carrier in Application Example 1 increased by 210.21%, and the skin retention amount increased by 436.16%. It shows that compared with the free composition, after the active ingredient is encapsulated by the idebenone anti-aging and skin brightening co-delivery carrier prepared by the present invention, it can effectively promote the transdermal absorption of the active ingredient and its retention amount in the skin, and improve its bioavailability.

[0122] Test Example 5: Cell antioxidant research Test samples: the carriers of Example 1 and Comparative Examples 1-7, and the free composition of Comparative Example 8.

[0123] HSF cells in the logarithmic growth phase were inoculated into 96-well plates at a density of 8×10 3 cells / well, 100 μL per well. After culturing for 24 h, the cells were divided into a control group, a model group, and an experimental group, with 3 replicates in each group. The control group was only added with DMEM complete medium, and the model group was added with 1.0 mmol / L H 2 O 2Complete DMEM medium. In the experimental groups, complete DMEM medium containing 1.0 mmol / L H 2 O 2 , and the test sample diluted 2000 times were added respectively. They were co-cultured for 24 h, and cell viability was measured using CCK-8. The experiment was repeated 3 times.

[0124] Table 1 Results of cell antioxidant assay The results are as Figure 3 shown in Table 1. After being damaged by 1.0 mmol / L H 2 O 2 , the cell viability of HSF cells in the model group was 49.2%, and the cell viability was significantly lower than that of the normal control group. Compared with the model group, experimental groups 1-9 supplemented with the carriers of Example 1, Comparative Examples 1-7, and the free composition of Comparative Example 8 could all significantly increase the cell survival rate (P<0.01).

[0125] Compared with experimental groups 1-3, experimental group 9 significantly increased the survival rate of HSF cells, and the difference was significant (P<0.01); it indicated that when the total weight parts of the anti-aging active ingredients were the same, the antioxidant effect of nano-carrier encapsulating three ingredients of idebenone, wolfberry extract, and peony root extract was better than that of nano-carrier encapsulating a single ingredient of idebenone, wolfberry extract, or peony root extract.

[0126] Compared with experimental group 9, in experimental group 6, wolfberry extract was replaced with grape seed extract, and the survival rate of HSF cells decreased significantly, indicating that any component replacement in the anti-aging composition of the present invention would affect the antioxidant effect. After the present invention compounded three specific components of idebenone, wolfberry extract, and peony root extract, the antioxidant effect was more significant (P<0.01).

[0127] Compared with experimental group 9, in experimental group 7, the weight ratio of idebenone, wolfberry extract, and peony root extract was not within the scope of the present invention, which also led to a decrease in the antioxidant effect and could not exert the best effect.

[0128] Compared with experimental group 8 (added with the free composition of Comparative Example 8), experimental group 9 could significantly increase the survival rate of HSF cells, and the difference was significant (P<0.01); it indicated that the anti-aging composition encapsulated by nano-carrier could significantly improve the antioxidant effect.

[0129] The experimental results proved that the compounding of idebenone, wolfberry extract, and peony root extract could significantly promote the effect of antioxidant active ingredients, showing a synergistic effect; and after being encapsulated by nano-carrier, the antioxidant effect of the active ingredient was significantly enhanced compared with the free active ingredient.

[0130] Test Example 6: Influence of Cell Secretion of Anti-aging Factor Collagen HSF cells were inoculated into a 24-well cell culture plate at a density of 1.0×10 5 cells / well, 0.5 mL per well. After culturing for 3 h, the samples were intervened respectively. In the experimental group, DMEM complete medium containing the anti-aging and brightening co-delivery carrier / free composition of Example 1 (idebenone) diluted 2000 times and Comparative Examples 1-8 was added, with 3 replicate wells in each group. The blank control group was only added with 500 µL of DMEM complete medium, with 3 replicate wells in each group. After pretreatment for 1 h, the cells were irradiated with UVA at 20 J / cm 2 , and then incubated in a CO 2 incubator for 24 h. The supernatant was taken, and the contents of ColⅠ and ColⅢ were tested with an Elisa kit. The results are shown in Table 2.

[0131] Table 2 Secretion Amount of Anti-aging Factor under Different Treatment Methods (n = 3) Note: Compared with the blank control group, ## p < 0.01; compared with the model group, * p < 0.05, ** p < 0.01; compared with Comparative Examples 1-3, && p < 0.01; compared with Comparative Examples 4 and 5, a p < 0.01; compared with Comparative Example 6, b p < 0.01; compared with Comparative Example 7, c p < 0.01; compared with Comparative Example 8, d p < 0.01.

[0132] As can be seen from the above table, compared with the blank control group, the contents of ColⅠ and ColⅢ secreted by the cells in the model group were significantly decreased after UVA irradiation (P < 0.01), indicating that the model was successfully constructed; compared with the model group, adding Example 1, Comparative Example 1, Comparative Example 3, Comparative Example 4, Comparative Example 6, Comparative Example 7, and Comparative Example 8 could significantly increase the secretion amounts of ColⅠ and ColⅢ (P < 0.05 or P < 0.01).

[0133] For the experimental groups adding the carriers of Comparative Example 2 and Comparative Example 6, there was no significant increase in the secretion amounts of ColⅠ and ColⅢ (P > 0.05), indicating that the single wolfberry extract could not promote the synthesis of collagen by cells. Compared with Example 1, Comparative Example 4, Example 1 only added wolfberry extract, which could significantly improve the ability of the anti-aging co-delivery carrier to secrete the anti-aging factor collagen. This shows that adding wolfberry extract can better promote the ability of idebenone and peony root extract to promote the synthesis of collagen by cells, and the three components in combination have a synergistic effect.

[0134] Compared with Comparative Examples 1-3, the co-delivery carrier of idebenone for anti-aging and skin brightening in Example 1 can significantly increase the contents of ColⅠ and ColⅢ secreted by cells (P<0.01), indicating that the co-delivery carrier of idebenone for anti-aging and skin brightening obtained by compounding idebenone with wolfberry extract and peony root extract has a better anti-aging effect than the co-delivery carrier of anti-aging and skin brightening prepared from a single active ingredient.

[0135] Compared with Comparative Examples 4 and 5, the contents of ColⅠ and ColⅢ secreted by the co-delivery carrier of idebenone for anti-aging and skin brightening prepared in Example 1 are increased by 373.3% and 171.12% respectively compared with the contents secreted by Comparative Examples 4 and 5, further indicating that the compounding of three raw materials of wolfberry extract, idebenone and peony root extract has a synergistic effect.

[0136] Compared with Comparative Example 6, the co-delivery carrier of idebenone for anti-aging and skin brightening in Example 1 can significantly increase the contents of ColⅠ and ColⅢ secreted by cells (P<0.01), indicating that the co-delivery carrier of idebenone for anti-aging and skin brightening obtained by compounding idebenone with wolfberry extract and peony root extract has a better anti-aging effect than the carrier obtained by compounding other plant extracts.

[0137] Compared with Comparative Example 7, the co-delivery carrier of idebenone for anti-aging and skin brightening in Example 1 can significantly increase the contents of ColⅠ and ColⅢ secreted by cells (P<0.01), indicating that the compounding of idebenone, wolfberry extract and peony root extract within a specific range has a more significant anti-aging effect (P<0.01); beyond the weight range of the present invention, a better anti-aging effect cannot be exerted.

[0138] Compared with Comparative Example 8 (free composition), the contents of ColⅠ and ColⅢ secreted by the co-delivery carrier of idebenone for anti-aging and skin brightening in Example 1 are significantly increased (P<0.01), indicating that the co-delivery carrier of idebenone for anti-aging and skin brightening in Example 1 has a better effect on promoting the secretion of anti-aging factors ColⅠ and ColⅢ than the free composition of the same dose.

[0139] The experimental results prove that the compounding of idebenone, wolfberry extract and peony root extract can significantly enhance the anti-aging effect of the active ingredients and has a synergistic effect; and after being encapsulated by the nano-carrier, the anti-aging effect of the active ingredient is significantly enhanced compared with the free active ingredient.

[0140] Test Example 7: Human anti-aging efficacy test Test samples: Essence liquids prepared by applying Example 1 and Comparative Examples 1-8.

[0141] The Shanghai SkinRevive Vplus® Intelligent Skin Analysis System and the MPA580 Skin Elasticity Tester were selected to conduct skin texture and skin elasticity tests, and comprehensively evaluate the anti-aging effect of the co-delivery carrier of idebenone for anti-aging and skin brightening.

[0142] Volunteers with healthy skin, no history of cosmetic allergies, and aged between 25 and 50 years old were selected as subjects. 30 subjects were randomly arranged in each group. After using the test samples, skin wrinkle and skin elasticity tests were carried out. The test sites were the two zygomatic regions of the face. Before the test, and after continuous use of the samples for 1 week, 2 weeks, and 4 weeks, the skin surface wrinkles and skin elasticity of the same subject's test site were detected, and the average reduction in surface wrinkles and the increase in skin elasticity value of the subjects at different time periods were calculated.

[0143] Table 3 Test results of the average reduction in surface wrinkles of subjects after using the test samples The test results of the average reduction in surface wrinkles of the subjects are shown in Table 3. The essence prepared with Application Example 1 (including the co-delivery carrier of idebenone for anti-aging and skin brightening in Example 1) can significantly reduce the amount of skin surface wrinkles, indicating that the co-delivery carrier of idebenone for anti-aging and skin brightening prepared by the present invention can effectively inhibit the growth of wrinkles and eliminate the original wrinkles, and has a significant anti-aging effect.

[0144] Compared with Application Comparative Examples 1-3 (including the co-delivery carrier of idebenone for anti-aging and skin brightening in Comparative Example 1, and the co-delivery carriers for anti-aging and skin brightening in Comparative Examples 2-3), after the subjects used the essence of Application Example 1, there was a significant difference in the change of the average reduction in surface wrinkles (P<0.01), indicating that the compound use of idebenone, wolfberry extract, and peony root extract has a synergistic effect, and the effect of inhibiting wrinkles is better than that of using high-concentration active ingredients alone.

[0145] Compared with Application Comparative Examples 4 and 5, after the subjects used the essence prepared with Application Example 1 (including the co-delivery carrier of idebenone for anti-aging and skin brightening in Example 1), the average reduction in surface wrinkles was higher than the sum of the average reduction in surface wrinkles of Comparative Example 4 and Comparative Example 5 (P<0.01), indicating that the compounding of wolfberry extract with idebenone and peony root extract has a synergistic effect.

[0146] Compared with Application Comparative Example 6, after the subjects used the essence of Application Example 1, there was a significant difference in the change of the average reduction in surface wrinkles (P<0.01), indicating that the anti-aging effect of the co-delivery carrier of idebenone for anti-aging and skin brightening obtained by compounding idebenone with wolfberry extract and peony root extract is better than that of the carrier obtained by compounding other plant extracts.

[0147] Compared with Application Comparative Example 7, after the subjects used the essence of Application Example 1, there was a significant difference in the change of the average surface wrinkle reduction amount (P<0.01), indicating that the co - formulation of idebenone, wolfberry extract and peony root extract within the specific range of the present invention has a more significant anti - aging effect (P<0.01).

[0148] Compared with Application Comparative Example 8 (including the free composition of Comparative Example 8), after the subjects used the essence prepared from Application Example 1 (including the idebenone anti - aging and skin - brightening co - delivery carrier of Example 1), there was a significant difference in the change of the average surface wrinkle reduction amount (P<0.01), indicating that the anti - aging active substances encapsulated by the nano - carrier can more effectively inhibit the growth of wrinkles, have a better improvement effect on wrinkles, and have an excellent anti - aging effect.

[0149] Table 4 Test results of the average skin elasticity increase amount after the subjects tried the test samples The test results of the average skin elasticity increase amount of the subjects are shown in Table 4. The essence prepared from Application Example 1 (including the idebenone anti - aging and skin - brightening co - delivery carrier of Example 1) can significantly increase skin elasticity, indicating that the idebenone anti - aging and skin - brightening co - delivery carrier prepared in the present invention can effectively stimulate the renewal of fibroblasts, thereby promoting the synthesis of collagen and elastin, enhancing skin elasticity, and promoting skin renewal.

[0150] Compared with Application Comparative Examples 1 - 3 (including the idebenone anti - aging and skin - brightening co - delivery carrier of Comparative Example 1 and the anti - aging and skin - brightening co - delivery carriers of Comparative Examples 2 - 3), after the subjects used the essence of Application Example 1, there was a significant difference in the change of the skin elasticity value increase amount (P<0.01), indicating that the co - use of idebenone, wolfberry extract and peony root extract has a synergistic effect, and has a better effect on increasing skin elasticity than using a high - concentration active ingredient alone.

[0151] Compared with Application Comparative Examples 4 and 5, after the subjects used the essence prepared from Application Example 1 (including the idebenone anti - aging and skin - brightening co - delivery carrier of Example 1), the increase amount of the skin elasticity value was higher than the sum of the increase amounts of the skin elasticity values of Comparative Example 4 and Comparative Example 5 (P<0.01), indicating that the co - formulation of wolfberry extract, idebenone and peony root extract has a synergistic effect.

[0152] Compared with Application Comparative Example 6, after the subjects used the essence of Application Example 1, there was a significant difference in the change of the skin elasticity value increase amount (P<0.01), indicating that the idebenone anti - aging and skin - brightening co - delivery carrier obtained by the co - formulation of idebenone, wolfberry extract and peony root extract has a better effect on increasing skin elasticity than the carrier obtained by the co - formulation of other plant extracts.

[0153] Compared with Application Comparative Example 7, after the subjects used the essence of Application Example 1, there was a significant difference in the change of the average reduction of surface wrinkles (P<0.01), indicating that idebenone, wolfberry extract and peony root extract were compounded within the specific range of the present invention, and the effect of increasing skin elasticity was more significant (P<0.01).

[0154] Compared with Application Comparative Example 8 (including the free composition of Comparative Example 8), after the subjects used the essence prepared from Application Example 1 (including the idebenone anti-aging and skin brightening co-delivery carrier of Example 1), there was a significant difference in the change of the increase in skin elasticity value (P<0.01), indicating that the active substances encapsulated by the nano-carrier could more effectively increase the skin elasticity value, promote the synthesis of collagen, tighten the skin, and have a more excellent anti-aging effect.

[0155] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An idebenone anti-aging and skin-lightening co-delivery carrier, characterized in that: The idebenone anti-aging and skin-lightening co-delivery carrier comprises a nanocarrier and an anti-aging composition encapsulated in the nanocarrier; the anti-aging composition comprises the following components in parts by weight: 1-5 parts of idebenone, 1-5 parts of wolfberry extract, and 1-5 parts of peony root extract; the nanocarrier comprises the following components: at least two of phospholipids, emulsifiers, co-emulsifiers, polyols, cholesterol, and water.

2. The idebenone anti-aging and skin-lightening co-delivery carrier according to claim 1, characterized in that: The nanocarrier comprises the following components in parts by weight: 1-5 parts of phospholipids, 10-50 parts of emulsifiers, 1-10 parts of auxiliary emulsifiers, 10-50 parts of polyols, 0.1-1 parts of cholesterol, and 20-50 parts of water.

3. The idebenone anti-aging and skin-lightening co-delivery carrier according to claim 1, characterized in that: The phospholipids include at least one of hydrogenated lecithin, lecithin, and soybean lecithin; And / or, the emulsifier includes at least one of polyoxyethylene castor oil emulsifier, polyoxyethylene hydrogenated castor oil emulsifier, polyglycerol emulsifier, poloxamer, and coconut glucoside; And / or, the auxiliary emulsifier includes at least one of laureth-23, diethylene glycol monoethyl ether, PPG-26-butanol polyether-26, trideceth-12, 1,3-propylene glycol, 1,2-pentanediol, 1,2-hexanediol, dipropylene glycol, glycerol, and butylene glycol; And / or, the polyol includes at least one of glycerol, ethanol, propylene glycol, butylene glycol, 1,3-propanediol, 1,2-pentanediol, 1,2-hexanediol, octyldodecanol, dipropylene glycol, isopropanol, ethoxydiglycol, polyethylene glycol-200, and PPG-10 sorbitol.

4. The idebenone anti-aging and skin-lightening co-delivery carrier according to claim 3, characterized in that: The phospholipid is lecithin; and / or, the emulsifier comprises PEG-40 hydrogenated castor oil, polyglyceryl-10 laurate and polysorbate-80; And / or, the co-emulsifier is PPG-26-butanol polyether-26; And / or, the polyol comprises octyldodecanol, ethoxydiglycol and 1,2-pentanediol.

5. The idebenone anti-aging and skin-lightening co-delivery carrier according to claim 2, characterized in that: The idebenone anti-aging and skin-lightening co-delivery carrier comprises the following components in parts by weight: 3 parts of idebenone, 3 parts of wolfberry extract, 3 parts of peony root extract, 1 part of phospholipids, 27 parts of emulsifier, 1 part of co-emulsifier, 10 parts of polyol, 0.5 part of cholesterol, and 35 parts of water.

6. A method for preparing the idebenone anti-aging and skin-lightening co-delivery carrier according to any one of claims 1 to 5, characterized in that: The steps include: (1) mixing idebenone, polyol, cholesterol and phospholipid, and stirring evenly to obtain a mixed solution A; (2) adding an emulsifier and a co-emulsifier to the mixed solution A obtained in step (1), and stirring the mixture to obtain a mixed solution B; (3) Mixing the wolfberry extract, the peony root extract and water, and stirring evenly to obtain a mixed solution C; (4) Add the mixed solution C obtained in step (3) to the mixed solution B obtained in step (2), mix, stir, and homogenize to obtain the idebenone anti-aging and skin-lightening co-delivery carrier.

7. Use of the idebenone anti-aging and skin-lightening co-delivery carrier according to any one of claims 1 to 5 in anti-aging cosmetics.

8. The use according to claim 7, characterized in that The dosage forms of the anti-aging cosmetics include water, milk and cream.

9. An anti-aging cosmetic, characterized in that: The invention comprises the idebenone anti-aging and skin-lightening co-delivery carrier according to any one of claims 1 to 5.

10. The anti-aging cosmetic according to claim 9, characterized in that: The amount of the idebenone anti-aging and skin-lightening co-delivery carrier added to cosmetics is 0.1-30%.

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