Blood protein peptide composition for promoting hair follicle reconstruction

Abstract

The invention relates to a blood protein peptide composition for promoting hair follicle reconstruction, and belongs to the technical field of cosmetics. The blood protein peptide composition disclosed by the invention comprises blood protein peptide, a hair follicle activator, a humectant, an emulsifier and an emollient, the blood protein peptide is composed of an MLL peptide fragment and a WDL peptide fragment. The amino acid sequence of the MLL peptide fragment is methionine-leucine-leucine, and the amino acid sequence of the MLL peptide fragment is methionine-leucine-leucine; the amino acid sequence of the WDL peptide fragment is tryptophan-aspartic acid-leucine. The blood protein peptide composition prepared by using the technical scheme of the invention can promote hair regeneration, shows good economic benefits and social benefits, is expected to promote industry progress, and leads the trend of a new generation of hair care products.

Description

Technical Field

[0001] This application relates to the technical field of cosmetics, and particularly to a blood protein peptide composition for promoting follicular reconstruction. Background Art

[0002] Currently, in the field of hair health and hair growth, various compounds have been explored for stimulating the hair follicle growth cycle, including minoxidil, ketoconazole, and other drugs. However, the effects of these compounds are limited and often accompanied by side effects. In addition, in recent years, platelet-rich plasma (PRP) therapy has received extensive attention due to its ability to promote tissue repair and regeneration. However, PRP relies on the extraction and processing of individual blood samples, which is costly and cumbersome.

[0003] Although there are a wide variety of hair growth promoting products on the market, most are based on a single active ingredient, lacking a compound formula synergistic mechanism, and many products are difficult to form a continuous and effective concentration distribution locally on the scalp. In addition, although the PRP-based treatment method is effective, due to the personalized preparation process, the cost is extremely high and the penetration rate is limited.

[0004] With the progress of bioengineering technology, it has become an urgent need to develop new hair health products with low cost, high efficiency, safety, and reliability. As a potential hair growth promoter, blood protein peptide has the advantages of wide sources and easy large-scale production, which provides a new idea for overcoming traditional technical bottlenecks and can solve the problems commonly existing in existing hair growth promoting products, such as unstable effects, uncertain long-term use safety, and high prices.

[0005] Therefore, in order to improve the local blood supply around the hair follicles and / or promote hair growth, it is urgent to develop a blood protein peptide composition with low cost, easy access, and good hair growth promoting effect. Summary of the Invention

[0006] The purpose of this application is to solve the problem of improving the local blood supply around the hair follicles and / or promoting hair growth, and to provide a blood protein peptide composition for promoting follicular reconstruction.

[0007] In a first aspect, this application provides a blood protein peptide composition, adopting the following technical solution: A blood protein peptide composition, comprising a blood protein peptide, a follicle activator, a humectant, an emulsifier, and an emollient; the blood protein peptide is composed of an MLL peptide segment and a WDL peptide segment; the amino acid sequence of the MLL peptide segment: methionine-leucine-leucine; the amino acid sequence of the WDL peptide segment: tryptophan-aspartic acid-leucine.

[0008] Optionally, the follicle activator is selected from one or more of BMP-4 and SHH.

[0009] Optionally, the composition further comprises human pluripotent stem cell exosomes.

[0010] Optionally, the humectant is sodium hyaluronate; the emulsifier is caprylic / capric triglyceride; the emollient is jojoba seed oil.

[0011] Optionally, the pH of the composition is 6.0 - 7.0.

[0012] Optionally, the dosage form of the composition is solution, hydroalcoholic solution, dispersion, suspension, hair growth liquid, emulsion, lotion, elixir, essence, toner, cleanser, cream, hair mask, mousse, ointment, gel, wax, oil, foam, soap, shampoo, conditioner, spray, aerosol, powder, paste.

[0013] In a second aspect, the present application provides an application of the above-mentioned blood protein peptide composition in the preparation of a cosmetic for promoting hair growth and / or follicle reconstruction.

[0014] In summary, the present application includes at least one of the following beneficial technical effects: 1) Significantly improved the speed of follicle reconstruction and the quality of hair regeneration, reduced the hair loss area, and promoted the growth of thick hair; 2) Optimized the formula so that blood protein peptides and other active substances are fully absorbed by the scalp, effectively improving the scalp environment; 3) Optimized the formula so that the blood protein peptides have good stability; 4) The newly added pluripotent stem cell exosomes and follicle activators synergistically cooperate with blood protein peptides to better promote hair regeneration. The overall technical solution shows good economic and social benefits, is expected to promote the progress of the industry, and lead the trend of a new generation of hair care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Showing the effect of the blood protein peptides of the present application in treating hair loss in mice. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0016] Preparation Example 1 Preparation of Blood Protein Peptides

[0017] The WDL peptide segment and the MLL peptide segment were respectively synthesized by solid-phase synthesis using an automatic peptide synthesizer (synthesized by Nanjing Yuan Peptide Biotechnology Co., Ltd.). The amino acid sequence of the MLL peptide segment: methionine-leucine-leucine, and the amino acid sequence of the WDL peptide segment: tryptophan-aspartic acid-leucine. They were purified by high-performance liquid chromatography (HPLC), and the synthesized peptides were identified by methods such as mass spectrometry (MS) and amino acid sequence analysis to confirm their sequences and purities. Finally, a peptide segment powder with a purity greater than 95% was obtained.

[0018] Preparation Example 2 Preparation of Human Pluripotent Stem Cell Exosomes

[0019] Coat a layer of embryonic stem cell matrix gel in a petri dish, transfer human induced pluripotent stem cells into the petri dish, add mTeSR1 serum-free medium, and culture at 37°C and 5% CO 2 2, saturated humidity, and collect the medium replaced daily. Centrifuge the culture supernatant at 300 g for 10 minutes at 4°C to remove cells, centrifuge at 2000 g for 10 minutes to remove debris; centrifuge at 10000 g for 30 minutes, and then filter through a 0.22 μm filter membrane to remove apoptotic bodies; centrifuge at 100000 g for 2 hours, resuspend the precipitate with PBS and centrifuge and wash again to obtain high-purity exosomes.

[0020] Example 1 Preparation of blood protein peptide composition

[0021] This example provides a composition with hair follicle protection effect, which is specifically prepared through the following steps: (1) Dissolve WDL peptide segment, MLL peptide segment, hair follicle activator BMP-4, and moisturizer sodium hyaluronate (molecular weight 3000 Da) in sterile water, and stir at 35°C and 1000 r / min for 45 min to obtain a homogeneous solution A; (2) Add emulsifier caprylic / capric triglyceride and emollient jojoba oil to solution A, and continue to stir evenly to obtain solution B; (3) Place the mixed solution stirred in step (2) in a high-pressure homogenizer (100 MPa, circulate 3 times), and homogenize for 20 min to form nanoparticles; (4) Adjust the pH to 6.5, sterilize through a 0.22 μm filter membrane, and finally obtain a composition with hair follicle protection effect; among them, the content of WDL peptide segment is 5%, the content of MLL peptide segment is 5%, the content of BMP-4 is 0.05%, the content of sodium hyaluronate is 20%, caprylic / capric triglyceride 3%, and the content of jojoba oil is 2%.

[0022] Example 2 Preparation of blood protein peptide composition

[0023] This example provides a composition with hair follicle protection effect, which is specifically prepared through the following steps: (1) Dissolve WDL peptide segment, MLL peptide segment, hair follicle activator BMP-4 and SHH, and moisturizer sodium hyaluronate (molecular weight 3000 Da) in sterile water, and stir at 35°C and 1000 r / min for 45 min to obtain a homogeneous solution A; (2) Add emulsifier caprylic / capric triglyceride and emollient jojoba oil to solution A, and stir evenly to obtain solution B; (3) Place the mixed solution after stirring in step (2) into a high-pressure homogenizer (100 MPa, 3 cycles) and homogenize for 20 min to form nanoparticles; (4) Adjust the pH to 6.5, sterilize through a 0.22 μm filter membrane, and finally obtain a composition with hair follicle protection effect; wherein, the content of WDL peptide segment is 5%, the content of MLL peptide segment is 5%, the content of BMP-4 is 0.03%, the content of SHH is 0.02%, the content of sodium hyaluronate is 20%, the content of caprylic / capric triglyceride is 3%, and the content of jojoba seed oil is 2%.

[0024] Example 3 Preparation of Blood Protein Peptide Composition

[0025] This example provides a composition with hair follicle protection effect, which is specifically prepared through the following steps: (1) Dissolve WDL peptide segment, MLL peptide segment, hair follicle activator BMP-4 and SHH, and moisturizer sodium hyaluronate (molecular weight 3000 Da) in sterile water, and stir at 35 °C and 1000 r / min for 45 min to obtain a uniformly stirred solution A; (2) Add emulsifier caprylic / capric triglyceride and emollient jojoba seed oil to solution A and stir evenly to obtain solution B; (3) Place the mixed solution after stirring in step (2) into a high-pressure homogenizer (100 MPa, 3 cycles) and homogenize for 20 min to form nanoparticles; (4) Add the human pluripotent stem cell exosomes of Preparation Example 2, adjust the pH to 6.5, sterilize through a 0.22 μm filter membrane, and finally obtain a composition with hair follicle protection effect; wherein, the content of WDL peptide segment is 5%, the content of MLL peptide segment is 5%, the content of BMP-4 is 0.03%, the content of SHH is 0.02%, the content of sodium hyaluronate is 20%, the content of caprylic / capric triglyceride is 3%, the content of jojoba seed oil is 2%, and the content of human pluripotent stem cell exosomes is 1%.

[0026] Example 4 Preparation of Blood Protein Peptide Composition

[0027] This example provides a composition with hair follicle protection effect, which is specifically prepared through the following steps: (1) Dissolve WDL peptide segment, MLL peptide segment, hair follicle activator BMP-4 and SHH, and moisturizer sodium hyaluronate (molecular weight 3000 Da) in sterile water, and stir at 35 °C and 1000 r / min for 45 min to obtain a uniformly stirred solution A; (2) Add emulsifier caprylic / capric triglyceride and emollient jojoba seed oil to solution A and stir evenly to obtain solution B; (3) Place the mixed solution after stirring in step (2) into a high-pressure homogenizer (100 MPa, circulate 3 times) and homogenize for 20 min to form nanoparticles; (4) Add the human pluripotent stem cell exosomes of Preparation Example 2, adjust the pH to 6.5, sterilize through a 0.22 μm filter membrane, and finally obtain a composition with hair follicle protection effect; wherein, the content of WDL peptide segment is 2%, the content of MLL peptide segment is 8%, the content of BMP-4 is 0.03%, the content of SHH is 0.02%, the content of sodium hyaluronate is 20%, the content of caprylic / capric triglyceride is 3%, the content of jojoba seed oil is 2%, and the content of human pluripotent stem cell exosomes is 1%.

[0028] Example 5 Preparation of blood protein peptide composition

[0029] This example provides a composition with hair follicle protection effect, which is specifically prepared through the following steps: (1) Dissolve WDL peptide segment, MLL peptide segment, hair follicle activator BMP-4 and SHH, and moisturizer sodium hyaluronate (molecular weight 3000 Da) in sterile water, and stir at 35 °C and 1000 r / min for 45 min to obtain a uniformly stirred solution A; (2) Add emulsifier caprylic / capric triglyceride and emollient jojoba seed oil to solution A and stir evenly to obtain solution B; (3) Place the mixed solution after stirring in step (2) into a high-pressure homogenizer (100 MPa, circulate 3 times) and homogenize for 20 min to form nanoparticles; (4) Add the human pluripotent stem cell exosomes of Preparation Example 2, adjust the pH to 6.5, sterilize through a 0.22 μm filter membrane, and finally obtain a composition with hair follicle protection effect; wherein, the content of WDL peptide segment is 8%, the content of MLL peptide segment is 2%, the content of BMP-4 is 0.03%, the content of SHH is 0.02%, the content of sodium hyaluronate is 20%, the content of caprylic / capric triglyceride is 3%, the content of jojoba seed oil is 2%, and the content of human pluripotent stem cell exosomes is 1%.

[0030] Example 6 In vivo function detection of blood protein peptide composition

[0031] 7-week-old male C57BL / 6 mice were used to establish an alopecia model with corticosteroid dexamethasone (modeling period 28 days).

[0032] Remove the hair in the resting phase on the back and use a depilatory cream to remove hair. Mice in the control group (abbreviated as NC in the attached figure) were topically applied with dexamethasone solution (100 nM, 0.1 mL, dissolved in propylene glycol) once a day in the depilated area for 30 consecutive days. Mice in the treatment group were applied with the blood protein peptide composition (Examples 1, 2, and 3 respectively, with an application amount of 10 mg / day) while applying the drug dexamethasone every day.

[0033] The experimental results are shown in Figure 1 , and the results were analyzed by skin color scores. The higher the score, the darker the color. Specifically as follows: Dark Grey 6 points; Grey 5 points; Greyish White 4 points; White 3 points; Pinky White 2 points; Pink 1 point. From the comparison results, it can be seen that the blood protein peptide composition of the present application can relieve the alopecia phenomenon in mice induced by the topical steroid dexamethasone.

[0034] Example 7 Detection of the stability of the blood protein peptide composition

[0035] An accelerated test was carried out on the blood protein peptide compositions of Examples 3-5. The test conditions were 40 °C / RH75%. HPLC analysis (C18 chromatographic column, mobile phase: 0.1 M phosphate buffer-acetonitrile gradient elution) was carried out every 3 months for the content of WDL peptide segments and the content of MLL peptide segments. The results of the 6-month accelerated test are shown in Table 1. The accelerated test results show that all indicators of the composition meet the quality standards within 6 months under the conditions of 40 °C / 75%RH.

[0036] Table 1

[0037] Those skilled in the art will further recognize that the present application can be implemented in other specific forms without departing from its spirit or central characteristics. Since the foregoing description of the present disclosure only discloses its exemplary embodiments, it should be understood that other variations are considered to be within the scope of the present application. Therefore, the present application is not limited to the specific embodiments described in detail herein. Instead, reference should be made to the appended claims to indicate the scope and content of the present application.

Claims

1. A blood protein peptide composition, characterized in that: It comprises a blood protein peptide, a hair follicle activator, a moisturizer, an emulsifier and a skin moisturizer; the blood protein peptide consists of an MLL peptide segment and a WDL peptide segment; the amino acid sequence of the MLL peptide segment is: methionine-leucine-leucine; the amino acid sequence of the WDL peptide segment is: tryptophan-aspartic acid-leucine.

2. A blood protein peptide composition according to claim 1, characterized in that: The hair follicle activator is selected from one or more of BMP-4 and SHH.

3. A blood protein peptide composition according to claim 1, characterized in that: The composition also includes human pluripotent stem cell exosomes.

4. A blood protein peptide composition according to claim 1, characterized in that: The moisturizing agent is sodium hyaluronate; the emulsifier is caprylic capric triglyceride; and the emollient is jojoba seed oil.

5. A blood protein peptide composition according to claim 1, characterized in that: The pH of the composition is 6.0-7.

0.

6. A blood protein peptide composition according to claim 1, characterized in that: The composition is in the form of a solution, hydroalcoholic solution, dispersion, suspension, hair tonic, emulsion, lotion, elixir, essence, toner, cleanser, cream, hair mask, mousse, ointment, gel, wax, oil, foam, soap, shampoo, conditioner, spray, aerosol, powder, paste.

7. Use of the blood protein peptide composition according to any one of claims 1 to 6 in the preparation of cosmetics for promoting hair growth and / or hair follicle reconstruction.

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