Preparation method of traditional Chinese medicine composition for tonifying qi and activating blood

Abstract

The invention relates to the technical field of modern traditional Chinese medicine preparations, in particular to a preparation method of a traditional Chinese medicine composition for tonifying qi and activating blood circulation, raw materials of the traditional Chinese medicine composition comprise conventional medicinal materials such as red ginseng, an active polysaccharide compound and a curcumin derivative, the medicinal materials are pretreated in multiple steps, then targeted enzymolysis fractionation, nanocrystallization treatment and intelligent mixing regulation are performed, and the traditional Chinese medicine composition for tonifying qi and activating blood circulation is prepared. And finally, embedding and molding the microcapsules to prepare various dosage forms. The application has obvious advantages, the innovative formula is added with new components to improve the curative effect, the advanced preparation process improves the purity and bioavailability of the components, the activity of the components can be protected, different medication requirements are met, and the modernization of traditional Chinese medicines is promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of modern traditional Chinese medicine preparations, and particularly to a preparation method of a traditional Chinese medicine composition for supplementing qi and activating blood circulation. Background Art

[0002] In the field of traditional Chinese medicine, traditional Chinese medicines for supplementing qi and activating blood circulation are of great significance for the treatment of various cardiovascular diseases, especially heart failure. The traditional concept of supplementing qi and activating blood circulation enhances cardiac function by supplementing qi to promote blood circulation, and at the same time activates blood circulation to eliminate blood stasis blockage, improve blood circulation, and relieve the symptoms of patients. However, with the in-depth development of modern medical research and the continuous improvement of clinical requirements, many problems have emerged in the existing traditional Chinese medicine compositions for supplementing qi and activating blood circulation and their preparation methods.

[0003] From the perspective of the medicinal materials themselves, the types of traditional medicinal materials used are relatively fixed, and the research on their components is not deep enough. For common medicinal materials such as red ginseng, salvia miltiorrhiza, ligusticum wallichii, notoginseng, and atractylodes lancea, although they have certain effects in supplementing qi and activating blood circulation, it is difficult to meet the complex and diverse disease requirements only relying on these conventional medicinal materials. At the same time, the extraction and utilization efficiency of the active ingredients in the medicinal materials is not high, and a large number of potential active ingredients have not been fully explored, which limits the therapeutic effect of the traditional Chinese medicine composition.

[0004] In terms of the preparation process, the traditional methods are relatively crude. Common pulverization methods can only pulverize the medicinal materials into relatively large particles, which makes it difficult for the active ingredients of the medicinal materials to be fully released in subsequent use, affecting the efficacy. For example, the particle size of the medicinal powder obtained by ordinary pulverization is relatively large, and during soaking or decocting, the dissolution rate of the active ingredients is slow and the dissolution amount is limited. Moreover, the traditional mixing process is difficult to ensure the uniform mixing of various medicinal material components, resulting in uneven quality of the final products and large differences in efficacy between different batches. During the mixing process, due to the lack of precise control means, the proportion of the medicinal materials may deviate, further affecting the stability of the therapeutic effect.

[0005] In addition, the existing traditional Chinese medicine preparation process rarely considers the active protection of the medicinal material components. Many active ingredients are easily inactivated by factors such as oxidation, temperature, and humidity during the preparation process. For example, the activities of some components with antioxidant and anti-inflammatory effects will be significantly reduced when exposed to high-temperature drying or long-term exposure to air, thereby reducing the overall efficacy of the traditional Chinese medicine composition.

[0006] With the progress of technology, although some preparation techniques have been improved, they still cannot meet the strict requirements of modern clinical practice for the quality and efficacy of traditional Chinese medicine. On the one hand, for newly discovered components or substances with potential qi-tonifying and blood-activating effects, they have not been well integrated into traditional Chinese medicine compositions, lacking innovative formula improvements. On the other hand, during the preparation process, the research on the synergistic effects between components is insufficient, and the comprehensive advantages of different components cannot be fully exerted, restricting the further development of traditional Chinese medicine in the field of cardiovascular disease treatment. Therefore, it is urgent to develop an innovative qi-tonifying and blood-activating traditional Chinese medicine composition and its advanced preparation method to improve the effect and quality of traditional Chinese medicine in the treatment of cardiovascular diseases such as heart failure. Summary of the Invention

[0007] (I) Technical Problems to be Solved In view of the deficiencies of the prior art, the present invention provides a preparation method for a qi-tonifying and blood-activating traditional Chinese medicine composition.

[0008] (II) Technical Solutions A preparation method for a qi-tonifying and blood-activating traditional Chinese medicine composition, wherein the traditional Chinese medicine composition is made of the following components by mass parts: 2-3 parts of red ginseng, 1-2 parts of salvia miltiorrhiza, 1-2 parts of ligusticum wallichii, 1-2 parts of notoginseng, 1-2 parts of atractylodes lancea, 0.5-1.5 parts of active polysaccharide complex, and 0.1-0.3 parts of curcumin derivative; the preparation method of the traditional Chinese medicine composition comprises the following steps: S1. Multi-step pretreatment: First, ultrasonic-assisted cleaning is performed on red ginseng, salvia miltiorrhiza, ligusticum wallichii, notoginseng, and atractylodes lancea, and after washing, they are dried in a far-infrared drying oven; then the dried medicinal materials are placed in an environment of -20 to -10 °C for freezing, and then frozen and pulverized; S2. Targeted enzymatic hydrolysis and fractionation: For the initially pulverized particles, enzymes are added for targeted enzymatic hydrolysis; ginseng saponin-specific enzyme is used for red ginseng, salvianolic acid enzyme is used for salvia miltiorrhiza, ligustrazine enzyme is used for ligusticum wallichii, notoginsenoside enzyme is used for notoginseng, and volatile oil-related enzyme is used for atractylodes lancea; after enzymatic hydrolysis, fractionation is performed by membrane separation technology, and the active ingredients in the molecular weight range of 100-500 Da are collected; S3. Nanonization treatment: Using supercritical fluid technology, with carbon dioxide as the supercritical fluid and adding an appropriate amount of ethanol as the entrainer, the active ingredients of each medicinal material after fractionation, as well as the active polysaccharide complex and curcumin derivative, are respectively prepared into nanoscale particles with a particle size controlled within 50-100 nm; S4. Intelligent mixing and activity regulation: An intelligent stirring device is used, and the parameters during the mixing process are monitored in real time through sensors; the nanoscale particles are added for mixing according to a preset ratio; at the same time, according to the real-time monitoring data, the stirring speed and stirring direction are automatically adjusted; S5. Microencapsulation and Molding: The mixed nanoparticles are microencapsulated using the microencapsulation technology with β-cyclodextrin and chitosan as the encapsulation materials and are encapsulated by spray drying; after encapsulation, if made into capsules, they are directly filled, if made into tablets, they are tabletted, and if made into granules, they are granulated.

[0009] Preferably, the active polysaccharide complex is compounded from lentinan and wolfberry polysaccharide in a ratio of 1:1 - 2:1, and the curcumin derivative is curcumin-polyethylene glycol-phospholipid complex, where the phenolic hydroxyl group of curcumin is connected to the terminal hydroxyl group of polyethylene glycol through an ether bond. The specific reaction formula is as follows: The hydrophobic part of curcumin binds to the fatty acid chain of phospholipid through hydrophobic interaction.

[0010] Preferably, the enzymatic hydrolysis process in S2 is carried out in a buffer solution system with a pH value of 6.0 - 7.5 to ensure the activity of the enzyme.

[0011] Preferably, the operating pressure of the supercritical fluid technology in S3 is 15 - 30 MPa to ensure the nanosizing treatment effect.

[0012] Preferably, the sensors of the intelligent stirring device in S4 include a near-infrared spectroscopy sensor for monitoring the component distribution, a humidity sensor with an accuracy of ±2%RH, and a temperature sensor with an accuracy of ±0.5°C.

[0013] Preferably, the spray flow rate during spray drying in S5 is 10 - 20 mL / min to ensure the microencapsulation effect.

[0014] Preferably, after the microencapsulation and molding step in S5, an accelerated stability test is carried out on the finished product for 7 - 10 days in an environment of 60°C and RH90%. The change in the component content is detected, and it can be packaged and shipped only after passing the test.

[0015] Preferably, during ultrasonic-assisted cleaning in S1, the ultrasonic power is 200 - 400 W to improve the cleaning efficiency.

[0016] Preferably, the ultrafiltration membrane with a molecular weight cut-off of 100 - 500 Da is used in the membrane separation technology in S2 to accurately separate the active ingredients.

[0017] Preferably, when making tablets in S5, the tabletting pressure is 3 - 5 MPa to ensure the hardness and quality of the tablets.

[0018] (III) Beneficial Technical Effects Compared with the existing technologies, the beneficial effects of the present invention are: 1. The newly added active polysaccharide complex and curcumin derivative greatly enhance the efficacy of the traditional Chinese medicine composition. The active polysaccharide complex regulates the body's immune function and can significantly enhance the antioxidant capacity of cardiomyocytes. By activating the MAPK pathway and upregulating the expression of antioxidant enzyme genes, it effectively reduces oxidative damage to cardiomyocytes and improves the heart's self-repair ability. The curcumin derivative, on the other hand, inhibits the release of inflammatory factors, improves myocardial microcirculation, provides a better blood supply environment for the heart, and reduces myocardial ischemia-hypoxia injury. These new components work synergistically with traditional herbs such as red ginseng and salvia miltiorrhiza to comprehensively improve the effect of the traditional Chinese medicine composition in treating heart failure.

[0019] 2. Steps such as multi-step pretreatment, targeted enzymatic hydrolysis, and fractionation improve the purity and extraction rate of the effective components of the herbs. Ultrasonic-assisted cleaning combined with cryogenic freezing and grinding can more thoroughly remove impurities while retaining the effective components of the herbs, and the preliminary ground particles after cryogenic grinding are more conducive to subsequent processing. Targeted enzymatic hydrolysis uses specific enzymes to precisely decompose the effective components in the herbs, and fractionation through membrane separation technology ensures the collection of effective components with high purity and a molecular weight range of 100 - 500 Da, improving the stability of product quality.

[0020] 3. Nanonization treatment reduces the particle size of the effective components of the herbs to 50 - 100 nm, greatly increasing their specific surface area and improving the bioavailability of the components. In vivo, nanoscale particles are more easily absorbed and can exert their medicinal effects faster. The intelligent mixing and activity regulation technology ensures the uniform mixing of the components, real-time monitoring, and automatic adjustment of parameters, not only ensuring the consistency of product quality but also maintaining the activity of the components and avoiding the problem of component inactivation in traditional preparation processes.

[0021] 4. The microcapsule embedding technology uses β-cyclodextrin and chitosan as embedding materials, effectively protecting the easily oxidized and degraded components and extending the shelf life of the product. Subsequent processing according to different dosage form requirements, such as making capsules, tablets, or granules, meets the diverse medication needs of patients. Overall, the preparation method of this application improves the quality and efficacy of the traditional Chinese medicine composition, provides a more effective drug option for the treatment of heart failure, and promotes the modernization development of traditional Chinese medicine. Description of the Drawings

[0022] Figure 1 is a flowchart of the preparation method of a traditional Chinese medicine composition for supplementing qi and activating blood circulation proposed by the present invention; Figure 2 is a line graph comparing the effective rate, marked effective rate, and ineffective rate of the examples and the control group in the treatment of heart failure; Figure 3 is a bar graph comparing the content of effective components of each herb in the examples and the control group; Figure 4It is a radar chart made after unifying the dimensions of the active ingredients of each medicinal material in the examples and comparative examples. Detailed implementation manners

[0023] According to Figures 1 to 4 , the detailed implementation manners of the present invention are as follows: Example 1

[0024] Raw material selection: Red ginseng was purchased from the ginseng planting base in Changbai Mountain, Jilin; Salvia miltiorrhiza was purchased from the GAP planting base of Salvia miltiorrhiza in Zhongjiang, Sichuan; Ligusticum chuanxiong was sourced from the standardized planting base of Ligusticum chuanxiong in Dujiangyan, Sichuan; Notoginseng was purchased from the Notoginseng Industrial Park in Wenshan, Yunnan; Atractylodes lancea was collected from the Atractylodes lancea planting base in Yingshan, Hubei. The polysaccharide raw material was purchased from Shaanxi Jiahe Biotechnology Co., Ltd., and the curcumin derivative was provided by Shanghai Yuanye Bio-Technology Co., Ltd.

[0025] Equipment description: Ultrasonic cleaning equipment: The KQ-500DE type numerical control ultrasonic cleaner of Kunshan Ultrasonic Instruments Co., Ltd. was used; Far-infrared drying oven: DHG-9070A type of Shanghai Yiheng Scientific Instruments Co., Ltd.; Freezing crusher: CJ-2000 ultra-low temperature crushing unit of Beijing Huanya Tianyuan Machinery Technology Co., Ltd.; Supercritical fluid equipment: HA121-50-02 type supercritical extraction device of Wuhan Huaxiang Kejie Biotechnology Co., Ltd.; Intelligent stirring equipment: A customized mixing system equipped with the NIR-S series near-infrared spectrum sensors of Endress+Hauser Company, Germany; Spray dryer: YC-015 experimental spray dryer of Shanghai Yacheng Instrument Equipment Co., Ltd.

[0026] Raw material preparation: Weigh 2 parts of red ginseng, 1 part of Salvia miltiorrhiza, 1 part of Ligusticum chuanxiong, 1 part of notoginseng, 1 part of Atractylodes lancea, 0.5 part of active polysaccharide complex (Lentinan and Lycium barbarum polysaccharide are compounded in a ratio of 1:1), and 0.1 part of curcumin derivative (curcumin-polyethylene glycol-phospholipid complex) by mass.

[0027] Multi-step pretreatment: Place each medicinal material in the ultrasonic cleaning equipment, wash it with deionized water, set the ultrasonic frequency to 30 kHz, and wash for 25 minutes. After washing, put it into the far-infrared drying oven and dry at 45 °C for 7 hours. Then put the dried medicinal materials into a low-temperature freezer at -20 °C and freeze for 2 hours. Subsequently, use a freezing crusher with a rotation speed of 3000 r / min to crush them to obtain preliminary crushed particles.

[0028] Targeted enzymatic hydrolysis and fractionation: The preliminarily crushed particles were placed in different enzymatic hydrolysis tanks. Ginseng was added with ginsenoside-specific enzyme, Salvia miltiorrhiza was added with salvianolic acid enzyme, Ligusticum chuanxiong was added with tetramethylpyrazine enzyme, Panax notoginseng was added with notoginsenoside enzyme, and Atractylodes lancea was added with volatile oil-related enzyme. Enzymatic hydrolysis was carried out at 35 °C for 4 hours in a buffer solution system with a pH value of 6.0. After enzymatic hydrolysis, membrane separation was carried out using an ultrafiltration membrane with a molecular weight cut-off of 100 Da, and the active ingredients within a specific molecular weight range were collected.

[0029] Nanometerization treatment: Using a supercritical fluid device, with carbon dioxide as the supercritical fluid and adding ethanol with a volume fraction of 5% as the entrainer, the active ingredients of each medicinal material after fractionation, as well as the active polysaccharide complex and curcumin derivative, were respectively prepared into nanoscale particles, with the particle size controlled within 50 - 100 nm.

[0030] Intelligent mixing and activity regulation: The nanoscale particles were put into an intelligent stirring device, which was equipped with a near-infrared spectroscopy sensor, a humidity sensor with an accuracy of ±2%RH, and a temperature sensor with an accuracy of ±0.5 °C. Under the conditions of a temperature of 25 °C and a humidity of 40%, stirring was carried out at a speed of 15 r / min for 40 minutes, and the stirring speed and direction were automatically adjusted according to the real-time monitoring data of the sensors.

[0031] Microencapsulation and shaping: Using β-cyclodextrin and chitosan (mass ratio 2:1) as the encapsulation material, encapsulation was carried out by spray drying method, with a spray flow rate of 10 mL / min, an inlet air temperature of 120 °C, and an outlet air temperature of 70 °C. After encapsulation, capsule agents were prepared.

[0032] Example 2

[0033] The raw material sources and equipment selections were the same as those in Example 1.

[0034] Raw material preparation: Weigh 2.5 parts of ginseng, 1.5 parts of Salvia miltiorrhiza, 1.5 parts of Ligusticum chuanxiong, 1.5 parts of Panax notoginseng, 1.5 parts of Atractylodes lancea, 1 part of active polysaccharide complex (a compound of lentinan and lycium barbarum polysaccharide in a ratio of 3:2), and 0.2 part of curcumin derivative.

[0035] Multi-step pretreatment: During ultrasonic cleaning, the ultrasonic frequency was 40 kHz, and the cleaning time was 20 minutes. The far-infrared drying temperature was 55 °C, and the drying time was 5 hours. The freezing temperature was -15 °C, and the freezing time was 1.5 hours. The rotational speed of cryogenic grinding was 4000 r / min.

[0036] Targeted enzymatic hydrolysis and fractionation: In a buffer solution system with a pH value of 6.8, enzymatic hydrolysis was carried out at 40 °C for 3 hours, and separation was carried out using an ultrafiltration membrane with a molecular weight cut-off of 300 Da.

[0037] Nanometerization treatment: In the supercritical fluid technology, the volume fraction of ethanol was 7%, and nanoscale particles were prepared.

[0038] Intelligent mixing and activity regulation: temperature 30°C, humidity 50%, stirring speed 25 r / min, stirring for 55 minutes.

[0039] Microcapsule embedding and forming: mass ratio of β-cyclodextrin to chitosan 2.5:1, spray flow rate 15 mL / min, inlet air temperature 130°C, outlet air temperature 80°C, made into tablets.

[0040] Example 3

[0041] The source of raw materials and the selection of equipment are the same as in Example 1.

[0042] Raw material preparation: Take 3 parts of red ginseng, 2 parts of salvia miltiorrhiza, 2 parts of ligusticum wallichii, 2 parts of notoginseng, 2 parts of atractylodes lancea, 1.5 parts of active polysaccharide complex (compounded by lentinan and wolfberry polysaccharide in a ratio of 2:1), and 0.3 part of curcumin derivative.

[0043] Multi-step pretreatment: ultrasonic frequency 50 kHz, cleaning for 15 minutes, far-infrared drying at 65°C for 4 hours, freezing at -10°C for 1 hour, and freezing and grinding speed 5000 r / min.

[0044] Targeted enzymatic hydrolysis and fractionation: in a buffer solution system with a pH value of 7.5, enzymatic hydrolysis at 45°C for 2 hours, and separation with an ultrafiltration membrane with a molecular weight cut-off of 500 Da.

[0045] Nanometerization treatment: ethanol volume fraction 10%, prepare nanoparticles.

[0046] Intelligent mixing and activity regulation: temperature 35°C, humidity 60%, stirring speed 35 r / min, stirring for 70 minutes.

[0047] Microcapsule embedding and forming: mass ratio of β-cyclodextrin to chitosan 3:1, spray flow rate 20 mL / min, inlet air temperature 140°C, outlet air temperature 90°C, made into granules.

[0048] Comparative example The source of raw materials and the selection of equipment are the same as in Example 1.

[0049] Raw material preparation: Weigh 2 parts of red ginseng, 1 part of salvia miltiorrhiza, 1 part of ligusticum wallichii, 1 part of notoginseng, and 1 part of atractylodes lancea.

[0050] Traditional treatment: Wash the medicinal materials with ordinary water, dry them at 60°C, and grind them with an ordinary grinder.

[0051] Mixing and forming: Simply mix the ground medicinal materials and add an appropriate amount of starch to make tablets.

[0052] Performance test and results Test data comparison The test results of Example 1, Example 2, Example 3 and the comparative example in terms of the active ingredient content, bioavailability and stability are as follows: Example 1: The active ingredient content is 28.5 mg / g, the bioavailability is 65%, and the retention rate of the active ingredient after the accelerated test is 90%.

[0053] Example 2: The active ingredient content is 32.1 mg / g, the bioavailability is 72%, and the retention rate of the active ingredient after the accelerated test is 92%.

[0054] Example 3: The active ingredient content is 35.8 mg / g, the bioavailability is 78%, and the retention rate of the active ingredient after the accelerated test is 95%.

[0055] Comparative example: The active ingredient content is 15.2 mg / g, the bioavailability is 30%, and the retention rate of the active ingredient after the accelerated test is 70%.

[0056] Result analysis Active ingredient content: The active ingredient contents of Examples 1 to 3 are significantly higher than those of the comparative example. Among them, the active ingredient content of Example 3 reaches 35.8 mg / g, which is about 2.3 times higher than that of the comparative example. This advantage is attributed to multi-step pretreatment technologies such as ultrasonic-assisted cleaning, far-infrared drying and cryogenic grinding, as well as the efficient extraction of active ingredients in medicinal materials by the targeted enzymatic hydrolysis process.

[0057] Bioavailability: The bioavailability ranges of Examples 1 to 3 are from 65% to 78%, which are much higher than 30% of the comparative example. The nanosizing technology controls the particle size of the active ingredient within 50 to 100 nm. Combining with the synergistic effect of curcumin derivatives and phospholipid complexes significantly improves the dissolution and absorption efficiency of the drug in vivo.

[0058] Stability: Examples 1 to 3 can still retain 90% to 95% of the active ingredient after the accelerated test, while the comparative example only retains 70%. The microcapsule embedding technology uses β-cyclodextrin and chitosan as embedding materials, effectively blocking the damage of light, heat and oxidation environment to the active ingredient.

[0059] The data of the active ingredient content and bioavailability of the examples and the comparative example are as follows in the table: Table 1 Item Example 1 Example 2 Example 3 Comparative Example Active ingredient of red ginseng (mg / g) 8.5 10.2 12.0 4.0 Active ingredient of danshen (mg / g) 6.2 7.5 8.8 3.0 Active ingredient of chuanxiong (mg / g) 4.8 5.6 6.5 2.5 Active ingredient of notoginseng (mg / g) 5.5 6.8 7.5 3.5 Active ingredient of atractylodes (mg / g) 3.5 4.0 4.5 2.0 Bioavailability (%) 65 72 78 30 Conclusion: This table details the comparison of the active ingredient contents of each medicinal material and the overall bioavailability between the examples and the comparative example. The active ingredient content and bioavailability of the examples are significantly higher than those of the comparative example, clearly demonstrating the advantages of the preparation method of this application in improving the quality and efficacy of traditional Chinese medicine compositions.

[0060] The comparison of the stability and efficacy between the examples and the comparative example is as follows in the table: Table 2 Item Example 1 Example 2 Example 3 Comparative Example Stability (retention rate of active ingredient after accelerated test %) 90 92 95 70 Effective rate for treating heart failure (%) 85 90 95 50 Marked effective rate (%) 40 45 50 20 Ineffective rate (%) 15 10 5 30 Conclusion: This table compares the stability and the efficacy in treating heart failure of the examples and the comparative examples. The examples have higher stability, and the effective rate and the marked effective rate are much higher than those of the comparative examples, while the ineffective rate is lower, fully demonstrating the remarkable achievements of the technology of this application in enhancing the product stability and treatment efficacy.

[0061] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation, characterized in that: The Chinese medicine composition is prepared from the following ingredients by weight: 2-3 parts of red ginseng, 1-2 parts of salvia miltiorrhiza, 1-2 parts of chuanxiong, 1-2 parts of panax notoginseng, 1-2 parts of atractylodes, 0.5-1.5 parts of active polysaccharide complex, and 0.1-0.3 parts of curcumin derivative; the preparation method of the Chinese medicine composition comprises the following steps: S1, multi-step pretreatment: firstly, red ginseng, salvia miltiorrhiza, chuanxiong, notoginseng, and atractylodes are cleaned with ultrasonic assistance, and then dried in a far-infrared drying oven; then, the dried medicinal materials are frozen in an environment of -20 to -10°C, and then frozen and crushed; S2. Targeted enzymatic hydrolysis and fractional separation: Enzymes are added to the initially crushed particles for targeted enzymatic hydrolysis; ginsenoside-specific enzymes are used for red ginseng, salvianolic acid enzymes are used for salvia miltiorrhiza, ligustrazine enzymes are used for ligusticum chuanxiong, notoginseng saponinase is used for Panax notoginseng, and volatile oil-related enzymes are used for Atractylodes lancea; after enzymatic hydrolysis, fractional separation is performed using membrane separation technology to collect effective components with a molecular weight range of 100-500Da; S3. Nano-processing: Using supercritical fluid technology, with carbon dioxide as supercritical fluid and an appropriate amount of ethanol as entrainer, the effective ingredients of each medicinal material after fractionation and separation, as well as active polysaccharide complexes and curcumin derivatives are prepared into nano-scale particles, with the particle size controlled at 50-100nm; S4, Intelligent mixing and activity regulation: Use intelligent mixing equipment to monitor the parameters of the mixing process in real time through sensors; add various nano-particles according to the preset ratio for mixing; at the same time, automatically adjust the stirring speed and stirring direction according to the real-time monitoring data; S5. Microcapsule encapsulation and molding: The mixed nanoparticles are encapsulated by microcapsule encapsulation technology, with β-cyclodextrin and chitosan as encapsulation materials, and are encapsulated by spray drying. After encapsulation, capsules are directly filled, tablets are compressed, and granules are granulated.

2. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: The active polysaccharide complex is prepared by mixing lentinan and wolfberry polysaccharide in a ratio of 1:1-2:

1. The curcumin derivative is a curcumin-polyethylene glycol-phospholipid complex, wherein the phenolic hydroxyl group of curcumin is connected to the terminal hydroxyl group of polyethylene glycol via an ether bond. The specific reaction formula is as follows: The hydrophobic part of curcumin binds to the fatty acid chains of phospholipids through hydrophobic interaction.

3. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: The enzymatic hydrolysis process in S2 is carried out in a buffer solution system with a pH value of 6.0-7.

5.

4. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: The operating pressure of supercritical fluid technology in S3 is 15-30MPa.

5. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: The sensors of the intelligent mixing equipment in S4 include a near-infrared spectroscopy sensor for monitoring ingredient distribution, a humidity sensor with an accuracy of ±2%RH, and a temperature sensor with an accuracy of ±0.5℃.

6. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: The spray flow rate during spray drying in S5 is 10-20 mL / min.

7. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: After the S5 microcapsule embedding and molding steps, the finished product is subjected to an accelerated stability test in an environment of 60°C and RH90% for 7-10 days to detect changes in component content.

8. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: During ultrasonic assisted cleaning in S1, the ultrasonic power is 200-400W.

9. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: The S2 membrane separation technology uses an ultrafiltration membrane with a molecular weight cutoff of 100-500Da.

10. The method for preparing a traditional Chinese medicine composition for invigorating qi and promoting blood circulation according to claim 1, characterized in that: When tablets are prepared in S5, the tableting pressure is 3-5 MPa.

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