Fluid fluid flow rate precision control device using synchronous operating dual valves and fluid

Abstract

The present invention relates to a microfluid flow rate precision control device using a synchronous operation double valve and a microfluid flow rate precision control method using the same, in order to precisely control the flow rate of a microfluid, the microfluid flow rate precision control device using the synchronous operation double valve and the microfluid flow rate precision control method using the same are set to be synchronous operation. Thus, the flow rate of the microfluid can be precisely controlled.

Description

Technical Field

[0001] The present invention relates to a microfluidic flow rate precision control device using a synchronous operation double valve and a microfluidic flow rate precision control method using the same. More specifically, the present invention relates to a microfluidic flow rate precision control device using a synchronous operation double valve that synchronously operates the flow rate of microfluid and a microfluidic flow rate precision control method using the same. Background Art

[0002] Microfluidic devices are used in various fields, such as biological sample analysis for various biochemical analyses and disease diagnoses. Microfluidic devices include micro-devices such as capillary channels, microfluidic chips, and lab-on-a-chip, and include microfluidic channels and microstructures.

[0003] The various types of microfluidic devices described above can be used to analyze specific components in chemical samples or biochemical samples, and can be used for culturing tissue cells in a micro-chamber, drug testing using the tissue cells, etc. Also, two or more sample solutions can be mixed in the microfluidic device to perform a chemical reaction, and can also be used for manufacturing functional materials, etc. Since microfluidic devices can be applied to various fields, microfluidic devices are used for various purposes in various fields.

[0004] The amount of the sample solution injected into the microfluidic channel of the microfluidic device is very small. Therefore, it is important to control the movement of the extremely small amount of sample solution to be injected into the microfluidic channel.

[0005] However, since the amount of the sample solution injected into the microfluidic channel of the microfluidic device is extremely small, it is important to control the movement of the extremely small amount of sample solution to be injected into the microfluidic channel, but there are the following practical difficulties. By applying pressure or vacuum to the fluid flowing through a conduit having a large diameter, the conveyance of the fluid can be easily controlled. However, in the case of the fluid in the microfluidic channel, the frictional force acting on the wall of the fluid channel is relatively very large. When a large pressure or vacuum for overcoming this frictional force is applied, the flow rate becomes very fast, and it is difficult to transfer a small amount of fluid.

[0006] The existing Korean Patent No. 10-2341588 (December 16, 2021) discloses a microfluidic regulation device and a microfluidic regulation method using the same. After forming a fluid leg between a first plate and a second plate, the fluid is inhaled through a flow channel, and the contact angle of the contact portions of the fluids respectively present on the first plate and the second plate is made smaller. Therefore, the fluid leg is separated, and thus the amount of the fluid can be precisely regulated.

[0007] However, the microfluidic control devices of the prior art have the problem of being difficult to precisely control the desired amount of fluid. Summary of the Invention

[0008] Technical Problem

[0009] Therefore, the present invention is proposed to solve the problems of the prior art as described above, and the object of the present invention is to provide a microfluidic flow rate precision control device using synchronous operation of double valves for precisely controlling the flow rate of microfluid and a microfluidic flow rate precision control method using the same.

[0010] Technical Solution

[0011] The microfluidic control device 1000 using synchronous double valves according to the present invention may include: a microfluidic tube 100 in the form of a capillary; a pressure transmission flow path 200 connected to one side of the microfluidic tube 100; a first synchronization unit connected to one side of the pressure transmission flow path 200; a second synchronization unit connected to the other side of the pressure transmission flow path 200; and a synchronization valve controller 600 connected in series with the first control unit and the second control unit.

[0012] Next, the microfluidic control method using double valves according to the present invention may include: a first synchronization step S100, connecting to one side of the pressure transmission flow path 200 connected to one side of the microfluidic tube 100, and controlling the first synchronization valve 310 through the synchronization valve controller 600 connected to the first wire 610; a second synchronization step S200, connecting to the other side of the pressure transmission flow path 200 connected to one side of the microfluidic tube 100, and controlling the second synchronization valve 320 through the synchronization valve controller 600 connected to the second wire 620.

[0013] Advantages of the Invention

[0014] Therefore, the microfluidic flow rate precision control device using synchronous operation of double valves of the present invention can achieve synchronous operation in which the first synchronization valve and the second synchronization valve operate with a specified time difference (δt), thereby being able to precisely control the flow of microfluid in the microfluidic tube.

[0015] In addition, the microfluidic flow rate precision control device using synchronous operation of double valves of the present invention can arbitrarily adjust the intensity and action time of the effective vacuum transmitted to the microfluidic tube connected to the pressure transmission flow path. Therefore, the flow of microfluid in the microfluidic tube can be precisely controlled, and various flow rates of microfluid can be obtained. Description of the Drawings

[0016] Figure 1 It is a schematic diagram of the microfluidic control device using synchronous operation of double valves of the present invention.

[0017] Figure 2 It is a flowchart of the microfluidic control method using a synchronous operation dual valve according to the present invention.

[0018] Figure 3 It is a schematic diagram of Embodiment 1 of the microfluidic control device using a synchronous operation dual valve according to the present invention.

[0019] Figure 4 It is a schematic diagram of Embodiment 2 of the microfluidic control device using a synchronous operation dual valve according to the present invention.

[0020] Figure 5 It is a synchronous operation chart according to the time difference between the first synchronization valve and the second synchronization valve of Embodiment 1 and Embodiment 2 of the microfluidic control device using a synchronous operation dual valve according to the present invention.

[0021] Figure 6 It is an experimental result of operating the microfluidic control device using a synchronous operation dual valve according to the present invention.

[0022] Figure 7 It is a practical photograph of the microfluidic control device using a synchronous operation dual valve according to the present invention. Detailed implementation mode

[0023] The microfluidic control device 1000 using a synchronous dual valve according to the present invention may include: a microfluidic tube 100 in the form of a capillary; a pressure transmission flow path 200 connected to one side of the microfluidic tube 100; a first synchronization unit connected to one side of the pressure transmission flow path 200; a second synchronization unit connected to the other side of the pressure transmission flow path 200; and a synchronization valve controller 600 connected in series with the first control unit and the second control unit.

[0024] In addition, the first control unit may include: a first synchronization valve 310 connected to one side of the pressure transmission flow path 200; a first pressure source 510 connected to the first synchronization valve 310 by a first wire to apply pressure; and the synchronization valve controller 600 connected to the first synchronization valve 310 by a first wire 610 to control the application time.

[0025] In addition, the second control unit may include: a second synchronization valve 320 connected to the other side of the pressure transmission flow path 200; a second pressure source 520 connected to the second synchronization valve 320 by a second wire 420 to apply pressure; and the synchronization valve controller 600 connected to the second synchronization valve 320 by a second wire 620 to control the application time.

[0026] In addition, the above synchronization valve controller can operate the first synchronization valve 310 and the second synchronization valve 320 at a predetermined time interval.

[0027] In addition, the above synchronization valve controller can operate the first synchronization valve 310 and the second synchronization valve 320 with a specified time difference (δt) within the range of 0 to ±100 ms.

[0028] In addition, the above microfluidic tube 100 can contain a fluid in the range of 0.1 to 500 μL.

[0029] In addition, the above first synchronization valve 310 can apply a negative pressure or a positive pressure to the pressure transmission flow path 200 by the operation of the above first pressure source 510.

[0030] In addition, the above second synchronization valve 320 can adjust the pressure applied to the inside of the pressure transmission flow path 200 by the operation of the above second pressure source 520.

[0031] Next, the microfluidic control method using a dual valve according to the present invention may include: a first synchronization step S100, connecting to one side of the pressure transmission flow path 200 connected to one side of the microfluidic tube 100, and controlling the first synchronization valve 310 through a synchronization valve controller 600 connected to a first wire 610; a second synchronization step S200, connecting to the other side of the pressure transmission flow path 200 connected to one side of the microfluidic tube 100, and controlling the second synchronization valve 320 through a synchronization valve controller 600 connected to a second wire 620.

[0032] In addition, the above first synchronization step S100 may include: a first pressure application step S110, applying pressure to the first synchronization valve 310 through a first pressure source 510 connected to the first synchronization valve 310 by a first wire; a first control step S120, after the above first pressure application step S110, controlling the time for applying pressure to the first synchronization valve 310 through a synchronization valve controller 600 connected to the first synchronization valve 310 by a first wire 610.

[0033] In addition, the above second synchronization step S200 may include: a second pressure application step S210, applying pressure to the second synchronization valve 320 through a second pressure source 520 connected to the second synchronization valve 320 by a second wire 420; a second control step S220, after the above second pressure application step S210, controlling the time for applying pressure to the second synchronization valve 320 through a synchronization valve controller 600 connected to the second synchronization valve 320 by a second wire 620.

[0034] In addition, the above-mentioned first synchronization step S100 and the above-mentioned second synchronization step S200 can cause the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 to operate with a specified time difference (δt) through the above-mentioned synchronization valve controller 600.

[0035] In addition, the above-mentioned first synchronization step S100 and the above-mentioned second synchronization step S200 can cause the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 to operate with a specified time difference (δt) in the range of 0 to ±100 ms through the above-mentioned synchronization valve controller 600.

[0036] The microfluid in the microfluidic device is subjected to positive or negative pressure (vacuum) due to the frictional force between the fluid tube wall, which has a relatively large impact on it, and the fluid, resulting in the problem that it is difficult to control the flow rate. In particular, if a sufficiently large pressure or vacuum is applied to overcome the frictional force, the flow rate becomes very fast, so that the flow rate cannot be accurately controlled to a low speed to process trace samples. This phenomenon may slow down the development of biochemical microanalysis techniques for processing trace samples and reagents. Therefore, the present invention uses a synchronization valve controller to control the synchronization of the synchronization valves in order to control the microfluid, thereby accurately controlling the flow of the microfluid in the microfluidic tube, and thus can solve the above-mentioned problems.

[0037] Hereinafter, the present invention will be described in more detail through specific examples or embodiments including drawings. However, the following specific examples or embodiments are only a reference for explaining the present invention in detail, and the present invention is not limited thereto and can be implemented in various ways.

[0038] In addition, unless otherwise defined, all technical terms and scientific terms have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. In the present invention, the terms used for explanation are only used to effectively describe specific specific examples and do not limit the present invention.

[0039] In addition, unless the context clearly indicates otherwise, the singular forms used in the specification and the appended claims are also intended to include the plural forms.

[0040] In addition, unless explicitly described to the contrary, a part "including" a certain component will be understood to also include other components without excluding other components.

[0041] Figure 1 is a schematic diagram of a microfluidic control device using synchronous double valves. Refer to Figure 1, the microfluidic control device 1000 using a synchronous double valve of the present invention includes: a microfluidic tube 100 in the form of a capillary; a pressure transmission flow path 200 connected to one side of the microfluidic tube 100; a first control unit and a second control unit, the first control unit being connected to one side of the pressure transmission flow path 200, and the second control unit being connected to the other side of the pressure transmission flow path 200; a synchronization valve controller 600 connected in series with the first control unit and the second control unit.

[0042] Among them, the microfluidic control device 1000 using the synchronous double valve can be used to precisely control the flow of the microfluid in the microfluidic tube 100. Therefore, one side of the microfluidic tube 100 can be connected to the pressure transmission flow path 200, and the other side can be immersed in the sample solution 10 in the sample solution 10 tank. Therefore, by using the microfluidic tube 100 and the operation of the microfluidic control device 1000 using the synchronous double valve, the micro-inhalation or discharge of the sample solution 10 can be precisely performed. Among them, the diameter of the microfluidic tube can be set to 0.01 to 1 mm. When the diameter of the microfluidic tube is less than 0.01 mm, it is necessary to increase the applied pressure. However, if the pressure is increased, the microfluid cannot be precisely controlled. In addition, when the diameter of the microfluidic tube is greater than 1 mm, the flow rate of the microfluid may be accelerated relative to the applied pressure, making it difficult to control the flow rate of the microfluid. Therefore, in the present invention, the diameter range of the microfluidic tube is designed to be 0.01 to 1 mm, so that the flow rate of the microfluid can be smoothly controlled.

[0043] In addition, the first control unit includes: a first synchronization valve 310 connected to one side of the pressure transmission flow path 200 to control the flow of the fluid; a first pressure source 510 connected to the first synchronization valve 310 by a first wire to apply pressure; and the synchronization valve controller 600 connected to the first synchronization valve 310 by a first wire 610 to control the applied pressure.

[0044] In addition, among them, the second control unit includes: a second synchronization valve 320 connected to the other side of the pressure transmission flow path 200 to control the flow of the fluid; a second pressure source 520 connected to the second synchronization valve 320 by a second wire 420 to apply pressure; and the synchronization valve controller 600 connected to the second synchronization valve 320 by a second wire 620 to control the applied pressure.

[0045] The above-mentioned synchronization valve controller can control the operation of the above-mentioned first synchronization valve 310 and the second synchronization valve 320. Among them, the above-mentioned first pressure source 510 and the above-mentioned second pressure source 520 may have different pressure intensities. The above-mentioned first pressure source 510 and the above-mentioned second pressure source 520 may be connected to the above-mentioned first synchronization valve 310 and the second synchronization valve 320 by a first wire and a second wire 420, and pressure can be applied through the above-mentioned first pressure source 510 and the second pressure source 520. In addition, through the control of the above-mentioned synchronization valve controller, the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 can operate with a specified time difference (δt). Therefore, by precisely adjusting the effective pressure intensity applied in the above-mentioned pressure transmission flow path 200, the flow of the trace fluid contained in the above-mentioned microfluidic tube 100 can be precisely controlled. Among them, the above-mentioned microfluidic tube 100 may include a fluid in the range of 0.1 to 500 μL. More specifically, the above-mentioned first pressure source 510 and the second pressure source 520 can have a mutual pressure difference by means of the above-mentioned synchronization valve controller, and apply a positive pressure or a negative pressure to the above-mentioned pressure transmission flow path 200. Thus, the flow rate of the microfluid can be controlled by adjusting the suction force of the above-mentioned microfluidic tube 100 connected to the above-mentioned pressure transmission flow path 200.

[0046] Figure 2 A step diagram showing the method for precisely controlling the microfluidic flow rate using a synchronously operated dual valve according to the present invention. Refer to Figure 3 For the method (S1000) for precisely controlling the microfluidic flow rate using a synchronously operated dual valve according to the present invention, it may include: a first synchronization step (S100), connecting to one side of the pressure transmission flow path 200 connected to one side of the microfluidic tube 100, and controlling the first synchronization valve 310 through a synchronization valve controller 600 connected to the first wire 610; a second synchronization step (S200), connecting to the other side of the pressure transmission flow path 200 connected to one side of the microfluidic tube 100, and controlling the second synchronization valve 320 through a synchronization valve controller 600 connected to the second wire 620.

[0047] Among them, the above-mentioned first synchronization step S100 may further include: a first pressure application step S110, applying pressure to the first synchronization valve 310 through a first pressure source 510 connected to the first synchronization valve 310 by a first wire; a first control step S120, after the above-mentioned first pressure application step S110, controlling the time of pressure application to the first synchronization valve 310 through a synchronization valve controller 600 connected to the first synchronization valve 310 by a first wire 610.

[0048] In addition, in the above-mentioned second synchronization step S200, it may further include: a second pressure application step S210 of applying pressure to the second synchronization valve 320 through a second pressure source 520 connected to the second synchronization valve 320 by a second wire 420; a second control step S220 of controlling the application time to the second synchronization valve 320 through a synchronization valve controller 600 connected to the second synchronization valve 320 by a second wire 620 after the above-mentioned second pressure application step S210.

[0049] Next, a method for precisely controlling the microfluidic flow rate using a synchronous operation dual valve according to the present invention will be described. In the above-mentioned first synchronization step S100, pressure can be applied to the first synchronization valve 310 through a first pressure source 510, and the application time of the pressure applied by the first pressure source 510 can be controlled through a synchronization valve controller 600. In addition, in the above-mentioned second synchronization step S200, pressure can be applied to the second synchronization valve 310 through a second pressure source 520, and the pressure applied by the second pressure source 520 can be controlled for the application time through a synchronization controller.

[0050] Therefore, through the above-mentioned synchronization valve controller 600, the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 can operate with a specified time difference (δt). More specifically, through the above-mentioned synchronization valve controller 600, the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 can operate with a specified time difference (δt) in the range of 0 to ±100 ms. Therefore, the vacuum degree can be adjusted with a small difference, so that the flow of the microfluid in the microfluidic tube 100 can be precisely controlled, and various flow rates of the microfluid can be obtained.

[0051] Figure 3 is a schematic diagram of Embodiment 1 of a microfluidic control device using a synchronous operation dual valve according to the present invention, Figure 4 is a schematic diagram of Embodiment 2 of a microfluidic control device using a synchronous operation dual valve according to the present invention.

[0052] Referring to the above Figure 3 and Figure 4 , specific descriptions will be given of Embodiment 1 and Embodiment 2 of the microfluidic flow rate precise control method for a microfluidic flow rate precise control device using a synchronous operation dual valve.

[0053] The above-mentioned first pressure source 510 uses an NF30-KFDC diaphragm pressure / vacuum pump of KNF Company. In the embodiment of the present invention, it is used by connecting a self-made plastic buffer container with a capacity of 100 mL. The vacuum degree of the above-mentioned second pressure source 520 (pressure / vacuum pump) is maintained at -55 to -65 kPa. At this time, the vacuum degree is measured using an MPX5100DP of NXP Semiconductor Company as a pressure / vacuum sensor.

[0054] The above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 respectively use the UMB1-T1 solenoid valve of CKD Corporation. The above-mentioned pressure transmission flow path uses a T-shaped connector to be connectable to the above-mentioned first synchronization valve and the above-mentioned second synchronization valve 320.

[0055] In addition, as Figure 3 shown, as Example 1, one side of the above-mentioned first synchronization valve 310 is connected to the above-mentioned first pressure source 510, and the other side is connected to the above-mentioned pressure transmission flow path 200. In addition, one side of the above-mentioned second synchronization valve 320 is connected to the above-mentioned pressure transmission flow path 200, and the other side is connected to form an atmospheric pressure inflow pipe 530 using a silicone tube with an outer diameter of 3 mm and an inner diameter of 1.0 mm and a transparent FEP tube with an outer diameter of 1.6 mm and an inner diameter of 0.5 mm.

[0056] In addition, among them, as Figure 4 shown, as Example 2, one side of the above-mentioned first synchronization valve 310 is connected to the above-mentioned pressure transmission flow path 200, and the other side is connected to the above-mentioned pressure transmission flow path 200. In addition, one side of the above-mentioned second synchronization valve 320 is connected to the above-mentioned second pressure source 520, and the other side is connected to form an atmospheric pressure inflow pipe 530 using a silicone tube with an outer diameter of 3 mm and an inner diameter of 1.0 mm and a transparent FEP tube with an outer diameter of 1.6 mm and an inner diameter of 0.5 mm.

[0057] In addition, the above-mentioned fluid microchannel 100 uses a PEEK tube with an inner diameter of 0.175 mm, an outer diameter of 1.6 mm, and a length of 60 mm.

[0058] In addition, in order to make the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 work with a specified time difference (δt), a synchronization valve controller 600 is used. In order to make the above-mentioned synchronization valve controller 600 work with a specified time difference, it is programmed through Arduino, and the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 are controlled through the above-mentioned synchronization valve controller 600 and signals are obtained. During this process, the above-mentioned first synchronization valve 310 and the above-mentioned second synchronization valve 320 turn on / off the 24V working power supply, so that the above two valves are synchronized.

[0059] In this embodiment, the opening operation time difference between the first synchronization valve 310 and the second synchronization valve 320 is varied between -10 ms and +10 ms. Here, when the opening time of the first synchronization valve 310 is earlier than the opening time of the second synchronization valve 320, the time difference (δt) is represented as a positive value. At this time, the first synchronization valve 310 can be connected to a vacuum source, and the second synchronization valve 320 can be connected to the atmosphere. Here, after applying a vacuum to the first synchronization valve 310, atmospheric pressure can be applied to the second synchronization valve 320. In this case, the vacuum applied to the first synchronization valve can be released into the atmosphere, and the second synchronization valve can be opened to the atmosphere to apply atmospheric pressure.

[0060] In addition, when the opening time of the first synchronization valve 310 is later than the opening time of the second synchronization valve 320, the time difference (δt) is represented as a negative value. At this time, the first synchronization valve 310 can be connected to the atmosphere, and the second synchronization valve 320 can be connected to a vacuum source. Here, after applying atmospheric pressure to the first synchronization valve 310, a vacuum can be applied to the second synchronization valve 320. At this time, the atmospheric pressure applied to the first synchronization valve 310 can be released, and a vacuum can be applied to the first synchronization valve.

[0061] In addition, in order to measure the moving speed of the microfluid in the microfluid tube 100, the other side of the microfluid tube 100 is immersed in the sample solution 10, where the sample solution 10 uses a black ink solution.

[0062] In the above-described embodiment, the results of measuring the moving speed of the sample solution 10 (black ink solution) in the microfluid tube 100 are classified into Example 1 and Example 2. For details, see . The synchronization operation charts of the time differences between the first synchronization valve and the second synchronization valve according to Example 1 and Example 2 of the microfluid control device using the synchronous operation double valve of the present invention are shown in Figure 5 。

[0063] <Example 1>

[0064] Referring to Figure 3 ,the first synchronization valve 310 is applied with a vacuum by being connected to a vacuum source, and the second synchronization valve is applied with atmospheric pressure by being connected to the atmosphere. Referring to Figure 5(a), after the first synchronization valve 310 connected to the vacuum source operates, the second synchronization valve 320 connected to the atmosphere can operate with a specified time difference (δt) to achieve synchronous operation. Referring to Example 1 of the following , after the first synchronization valve 310 connected to the vacuum source operates, the second synchronization valve 320 connected to the atmosphere operates with a specified time difference (δt), thereby increasing the suction flow rate per second of the microfluidic tube. Specifically, it can be seen that as the time difference changes from 10 to -10 ms, the suction flow rate of the sample solution sucked into the microfluidic tube 100 becomes smaller. Among them, the first synchronization valve 310 can be connected to the vacuum source, and the second synchronization valve 320 can be connected to the atmosphere. After the vacuum source is applied to the first synchronization valve 310, atmospheric pressure can be applied to the second synchronization valve 320. Therefore, by applying a very weak vacuum, an extremely small amount of fluid can be sucked, enabling very fine control.

[0065] <Example 2>

[0066] Referring to Figure 4 , the first synchronization valve 310 is connected to the atmosphere to apply atmospheric pressure, and the second valve is connected to the vacuum source to apply vacuum. Referring to Figure 5 (b), after the first synchronization valve 310 connected to the atmosphere operates, the second synchronization valve 320 connected to the vacuum source can operate with a specified time difference (δt). Referring to Example 2 of the following , after the first synchronization valve 310 connected to the atmosphere operates, the second synchronization valve 320 connected to the vacuum source operates with a specified time difference (δt) remaining, thereby reducing the suction flow rate per second of the microfluidic tube. Thus, the flow of a very small amount of microfluid can be controlled by reducing the suction flow rate per second. Specifically, it can be seen that as the time difference changes from 0 to 10 ms, the suction flow rate of the sample solution sucked into the microfluidic tube increases. Here, the first synchronization valve 310 is connected to the atmosphere, and the second synchronization valve 320 is connected to the vacuum source, so that vacuum can be applied. Therefore, after applying the vacuum source to the first synchronization valve 310, a stronger vacuum can be applied compared to when atmospheric pressure is applied to the second synchronization valve 320. Therefore, a relatively larger amount of trace fluid can be sucked, enabling the control of the flow rate of microfluid.

[0067] As shown in Example 1 and Example 2 of below, it was confirmed that when the time difference (δt) changed from -10 ms to 10 ms, the suction flow rate of the fluid sucked into the microfluidic tube 100 could change from 0.15 μL / s to 1.54 μL / s. Therefore, as the first synchronization valve and the second synchronization valve are connected to the atmosphere or a vacuum source, the intensity and action time of the effective vacuum transmitted to the microfluidic tube 100 connected to the pressure transmission flow path 200 can be arbitrarily adjusted. Thereby, the microfluid flow in the microfluidic tube 100 can be precisely controlled.

[0068]

[0069]

[0070] Using the microfluidic control device 1000 with synchronous double valves of the present invention, it was confirmed that the suction flow rate of several μL of microfluid in the microfluidic tube 100 can be precisely controlled by applying vacuum and atmospheric pressure.

[0071] Figure 6 Showing the experimental results of operating the microfluidic control device with synchronous double valves of the present invention, Figure 7 is a practical photo of the microfluidic control device with synchronous double valves of the present invention, showing the practical photo of the microfluidic control device with synchronous double valves of the present invention. As Figure 6 and Figure 7 shown, by using the microfluidic flow rate precision control device and method with synchronous double valves of the present invention, it is possible to achieve the synchronous operation of the first synchronization valve 310 and the second synchronization valve 320 working with a specified time difference (δt), thereby precisely controlling the microfluid flow in the microfluidic tube 100.

[0072] In addition, the intensity and action time of the effective vacuum transmitted to the microfluidic tube 100 connected to the pressure transmission flow path 200 can be arbitrarily adjusted. Therefore, the flow of the microfluid in the microfluidic tube 100 can be precisely controlled, and the flow rate of the microfluid can be diversely adjusted according to the application.

[0073] Therefore, in various fields of microfluids such as microanalysis and microreactions that require trace amounts, by precisely controlling the transfer of extremely trace samples in the microfluidic channel, it is possible to easily and simply control the flow rate of the microfluid, and there is no need for a separate special-purpose device or component for controlling the flow rate of the microfluid. Therefore, it has the effect of being able to simply and economically achieve precise control of the flow rate of the microfluid.

[0074] The present invention is not limited to the above embodiments, and has a wide range of applications. Without departing from the gist of the present invention claimed in the claims, various modifications can be made.

[0075] Industrial applicability

[0076] The microfluidic flow rate precision control device using synchronous operation of double valves and the microfluidic flow rate precision control method using the same according to the present invention can easily and simply control the flow rate of microfluidics in fields requiring various microfluidics, and can achieve precise control of the flow rate of microfluidics simply and economically.

Claims

1. A microfluidic control device using synchronous dual valves, comprising: a microfluidic tube in the form of a capillary; a pressure transfer flow path connected to one side of the microfluidic tube; a first synchronization unit connected to one side of the pressure transfer flow path; a second synchronization unit connected to the other side of the pressure transfer flow path; and a synchronization valve controller connected in series with the first control unit and the second control unit.

2. The microfluidic control device using synchronous dual valves according to claim 1, wherein, the first control unit includes: a first synchronization valve connected to one side of the pressure transfer flow path; a first pressure source connected to the first synchronization valve by a first wire to apply pressure; and a synchronization valve controller connected to the first synchronization valve by a first wire to control the application time.

3. The microfluidic control device using synchronous dual valves according to claim 1, wherein, the second control unit includes: a second synchronization valve connected to the other side of the pressure transfer flow path; a second pressure source connected to the second synchronization valve by a second wire to apply pressure; and the synchronization valve controller connected to the second synchronization by a second wire to control the application time.

4. The microfluidic control device using synchronous dual valves according to claim 1, wherein, the synchronization valve controller operates the first synchronization valve and the second synchronization valve at a preset time interval.

5. The microfluidic control device using synchronous dual valves according to claim 2, wherein, the synchronization valve controller operates the first synchronization valve and the second synchronization valve with a specified time difference (δt) within the range of 0 to ±100 ms.

6. The microfluidic control device using synchronous dual valves according to claim 1 above, wherein, the microfluidic tube contains a fluid in the range of 0.1 to 500 μL.

7. The microfluidic control device using synchronous dual valves according to claim 1, wherein, the first synchronization valve applies a negative pressure or a positive pressure to the pressure transfer flow path by the operation of the first pressure source.

8. The microfluidic control device using dual valves according to claim 1, wherein, the second synchronization valve adjusts the pressure applied into the pressure transfer flow path by the operation of the second pressure source.

9. The microfluidic control method using dual valves according to any one of claims 1 to 8, comprising: a first synchronization step of connecting to one side of the pressure transfer flow path connected to one side of the microfluidic tube and controlling the first synchronization valve through a synchronization valve controller connected by a first wire; and a second synchronization step of connecting to the other side of the pressure transfer flow path connected to one side of the microfluidic tube and controlling the second synchronization valve through a synchronization valve controller connected by a second wire.

10. The microfluidic control method using dual valves according to claim 9, wherein, the first synchronization step includes: a first pressure application step of applying pressure to the first synchronization valve through a first pressure source connected to the first synchronization valve by a first wire; and The first control step, after the above first pressure application step, controls the time applied to the first synchronization valve by a synchronization valve controller connected to the first synchronization valve by a first wire.

11. The microfluidic control method using a dual valve according to claim 9, wherein, the above second synchronization step includes: a second pressure application step of applying pressure to the second synchronization valve by a second pressure source connected to the second synchronization valve by a second wire; and a second control step of controlling the time applied to the second synchronization valve by a synchronization valve controller connected to the second synchronization valve by a second wire after the above second pressure application step.

12. The microfluidic control method using a dual valve according to claim 9, wherein, in the above first synchronization step and the above second synchronization step, the above first synchronization valve and the above second synchronization valve are operated with a specified time difference (δt) by the above synchronization valve controller.

13. The microfluidic control method using synchronized dual valves according to claim 12, wherein, in the above first synchronization step and the above second synchronization step, the above first synchronization valve and the above second synchronization valve are operated with a specified time difference (δt) in the range of 0 to ±100 ms by the above synchronization valve controller.

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