Biological cell regulating fluid, preparation method and application of biological cell regulating fluid in anti-inflammatory, antiviral and immune regulation aspects

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a biological cell regulating solution, a preparation method of the regulating solution and application of the regulating solution in the aspects of virus resistance, inflammation resistance and immune regulation. The method has the advantages that by accurately controlling the dilution concentration of the electromagnetic base liquid and key parameters such as the reduction potential and the pH value of the diluent, it is ensured that hydration electrons in the diluent can form a stable quantum state structure, and a foundation is laid for the subsequent biological regulation function. The biological cell regulation liquid is applied to anti-inflammatory, anti-virus and immune regulation, the function that one liquid has multiple purposes is achieved, overlapped medication is not needed, and the medicine has the advantages of being efficient in virus resistance (the virus inactivation rate exceeds 95%), high in selectivity (not affecting normal cells), rapid in effect, lasting and stable in regulation effect, free of excessive immune reaction risk and the like; in addition, the functions of various immune cells can be synchronously enhanced in the aspect of immune regulation, and comprehensive immune regulation is truly realized. In the anti-inflammatory aspect, accurate anti-inflammation can be achieved, the inflammatory factor level is remarkably reduced, the normal physiological function is not affected, the biocompatibility is good in use, and no accumulative side effect exists.

Description

Technical Field

[0001] The present invention belongs to the field of biomedical technology. Specifically, it relates to a biological cell regulating fluid, a preparation method of the regulating fluid, and applications of the regulating fluid in anti-virus, anti-inflammatory, and immune regulation aspects. Background Art

[0002] In the field of biomedicine, effectively regulating the immune function of organisms has been a long-term research challenge. Traditional immune regulation methods mainly include immune enhancers, cytokine therapy, and monoclonal antibodies, etc. These methods have several key problems in clinical applications: 1. Insufficient selectivity, which often affects normal cell functions; 2. Unstable immune regulation effects, which are prone to cause overreactions of the immune system; 3. Poor biocompatibility, which may produce serious side effects; 4. Single action mechanism, making it difficult to achieve multi-level coordinated regulation.

[0003] With the development of quantum chemistry and molecular biology, researchers have gradually realized that the redox balance at the cellular level plays an important regulatory role in immune function. Especially during the inflammatory response and viral infection processes, the redox state of cells directly affects the activity and function of immune cells.

[0004] However, how to precisely regulate the cellular redox state while maintaining sufficient selectivity and safety has always been a major challenge in this field.

[0005] Current redox regulation technologies have the following deficiencies: 1. Low regulation precision, making it difficult to achieve precise control at the molecular level; 2. Short action duration, requiring frequent drug administration; 3. Lack of selectivity, prone to causing damage to normal tissues; 4. Unclear mechanism, making it difficult to conduct targeted optimization. These problems severely restrict the application of related technologies in clinical practice.

[0006] In addition, existing technologies are often fragmented in anti-virus and immune regulation aspects, lacking a unified action mechanism and synergistic effect. This results in the need to use multiple drugs in combination in practical applications, increasing the complexity of treatment and the risk of side effects. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to provide a new drug that can simultaneously achieve anti-virus, immune regulation, and anti-inflammatory effects and has good biocompatibility.

[0008] To solve the above technical problems, the technical solution adopted by the present invention is as follows: A biological cell regulating solution, characterized in that it comprises an electromagnetic base solution and deionized water. The electromagnetic base solution is the electromagnetic base solution prepared in accordance with the patent US 11691906B1. The mass ratio of deionized water to the electromagnetic base solution is 1000:1 - 2000:1. The pH value range of the dilution solution is 10 - 12, and the ORP value is -200 mV to -100 mV.

[0009] The stable electromagnetic base solution described in the US 11691906B1 patent is essentially a stable hydrated electron base solution. When the electromagnetic base solution is diluted in large amounts with deionized water, under the conditions of a specific concentration range and reduction potential, the hydrated electrons (eaq-) are surrounded by water molecules and can form a unique quantum state structure. This structure has three key features: First, throughout the dilution process, the strength of the hydrogen bonds in the liquid changes from strong to weak, then from weak to strong, and finally an ordered long-range hydrogen bond network is formed between water molecules. This hydrogen bond network not only has a high hydrogen bond strength but also a very stable state, providing a stable quantum channel for electron transfer. Second, through the quantum tunneling effect, precise recognition and action on specific molecular structures are achieved. Third, the reduction potential of the system is precisely controlled within a special energy window, which can effectively act on target molecules without interfering with normal cell functions. The present invention realizes a triple synergistic action mechanism at the molecular level and achieves multi-level biological regulation functions through precise quantum chemical processes.

[0010] According to one aspect of the present invention, an antiviral solution, the therapeutic component of which comprises the biological cell regulating solution. This is the application of the biological cell regulating solution in antiviral treatment. The antiviral effect is achieved through the quantum tunneling effect between hydrated electrons and viral surface proteins, which changes the charge distribution on the virus surface and inhibits its ability to bind to cell receptors.

[0011] According to another aspect of the present invention, an immune regulating solution, the therapeutic component of which comprises the biological cell regulating solution. This is the application of the biological cell regulating solution in immune regulation. The immune regulation effect is achieved by optimizing the cellular redox environment, enhancing the killing activity of NK cells, the proliferation ability of T lymphocytes, and the phagocytic function of macrophages.

[0012] According to another aspect of the present invention, an anti-inflammatory solution, the therapeutic component of which comprises the biological cell regulating solution. This is the application of the biological cell regulating solution in anti-inflammatory treatment. The anti-inflammatory effect is achieved by scavenging excessive reactive oxygen species, regulating the production of inflammatory factors, and optimizing the cellular redox balance.

[0013] A preparation method of a biological cell regulating solution, comprising the following steps: Step 1: Under an inert atmosphere, deionized water is used to dilute an electromagnetic base liquid, and the electromagnetic base liquid is the electromagnetic base liquid prepared in US 11691906B1 patent. The mass ratio of deionized water to the electromagnetic base liquid is 1000:1 - 2000:1; Step 2: When it is detected that the pH value of the liquid is 10 - 12 and the ORP value is -200 mV to -100 mV, stop dilution; Step 3: After dilution is completed, let it stand for at least 24 hours, and the hydrogen bond network in the liquid is reorganized to form a stable long-range ordered structure. Infrared spectrum analysis shows that before standing, the stretching peak of hydroxyl -OH is located at 3420 ± 15 cm⁻¹, and the peak width is 280 cm⁻¹; after standing for 24 hours, the peak value shifts to 3280 ± 10 cm⁻¹, and the peak width decreases to 140 cm⁻¹, indicating that the order degree of the hydrogen bond network is significantly improved, promoting the formation of a more stable quantum state structure of hydrated electrons in the liquid. During the whole dilution process, precise control of key parameters such as the concentration, reduction potential, and pH value of the dilution liquid is required to ensure that the dilution liquid can form a stable quantum state structure, laying a foundation for subsequent biological regulation functions. After dilution is completed, the stretching peak value of hydroxyl -OH is less than that of the original liquid, but this is not the final formed ordered long-range hydrogen bond network. However, after standing for 24 hours or longer, a very stable structural state will finally be formed, and then it can have good cell regulation efficacy.

[0014] Preferably, the ORP value of the regulating solution is -150 mV.

[0015] Preferably, in Step 2, the pH value range of the liquid is 11.5 - 12.

[0016] The advantages of the present invention are as follows: By precisely controlling the concentration of the electromagnetic base liquid dilution, as well as key parameters such as the reduction potential and pH value of the dilution liquid, it is ensured that the hydrated electrons in the dilution liquid can form a stable quantum state structure, laying a foundation for subsequent biological regulation functions. By applying the biological cell regulating solution to anti-inflammatory, anti-viral, and immune regulation, the function of using one liquid for multiple purposes is realized, and there is no need for overlapping medication. This medicine has the advantages of high-efficiency anti-virus (virus inactivation rate exceeds 95%), high selectivity (does not affect normal cells), rapid action, long-lasting and stable regulation effect, no risk of excessive immune response, etc. Moreover, in terms of immune regulation, it can simultaneously enhance the functions of multiple immune cells, truly realizing comprehensive immune regulation. In terms of anti-inflammatory, it can achieve precise anti-inflammatory, significantly reduce the level of inflammatory factors without affecting normal physiological functions, and has good biocompatibility and no cumulative side effects during use. Description of the Drawings

[0017] Figure 1 : Schematic diagram of the quantum state structure of hydrated electrons Figure 2 : Quantum tunneling process between hydrated electrons and the virus surface Figure 3 : Process of interaction between hydrated electrons and viruses Figure 4 : Immunomodulation effect diagram Figure 5 : Anti-inflammatory mechanism diagram Figure 6 : Technical comprehensive evaluation diagram Specific implementation manner

[0018] For ease of understanding the present invention, the present invention will be described in more detail below in conjunction with the accompanying drawings and specific embodiments.

[0019] Example 1: Anti-virus (stable period) The biological cell regulating solution used in this example is obtained through the following method: Step 1: Under an inert atmosphere, deionized water is used to dilute the electromagnetic base liquid. The electromagnetic base liquid is the electromagnetic base liquid prepared in US 11691906B1 patent. The mass ratio of deionized water to the electromagnetic base liquid is 1000:1; Step 2: The pH value of the diluted liquid is 12, and the ORP value is -150 mV. The dilution is ended; Step 3: After dilution, it is left standing for 24 hours. By detecting the stretching peak of hydroxyl -OH in the regulating solution through infrared spectroscopy at 3280 ± 10 cm⁻¹, the biological cell regulating solution required for this example is obtained.

[0020] Through an electronic state structure analysis system and a quantum tunneling effect detection device (in this example, Thermo Fisher IS20 is used), the quantum state characteristics of hydrated electrons in the regulating solution are monitored, including key indicators such as the order degree of the hydrogen bond network structure, electron transfer efficiency, and molecular recognition accuracy, to ensure that the hydrated electrons always maintain the best active state.

[0021] The virus used in this example is the standard strain of human influenza virus A (H1N1 A / Puerto Rico / 8 / 34). The virus concentration kit is obtained from ATCC in the United States.

[0022] The virus suspension (10^6 TCID50 / mL) is divided into an experimental group and a control group. The experimental group is added with the cell regulating solution at a volume ratio of 1:10 and incubated at 37°C for 30 minutes.

[0023] After incubation, a JEM-2100F transmission electron microscope was used to observe the morphological changes of the virus, and a Zetasizer Nano ZS analyzer was used to monitor the changes in the surface charge distribution of the virus. Experimental observations showed that hydrated electrons in the cell conditioning solution interacted with the viral surface proteins within milliseconds through quantum tunneling effects. Transmission electron microscope observations revealed significant changes in the surface structure of the treated virus, and the surface charge distribution was significantly altered. Virus titer determination indicated that the virus infectivity decreased by more than 97%, while normal cells in the control group were not affected.

[0024] From the above experiments, it can be seen that: As Figure 1 shown, on the left is the arrangement of water molecules and the hydrogen bond network in the initial or normal state, and on the right is the ordered long-range hydrogen bond network structure formed after treatment. The ordered hydrogen bond network formed between water molecules creates a special environment that enables electrons to transition between molecular orbitals. This ordered structure reduces the energy barrier required for electron transition, provides a directional channel, and makes electron transfer more efficient and controllable.

[0025] This is one of the key mechanisms by which the conditioning solution functions. By forming a stable long-range hydrogen bond network, it creates ideal conditions for the quantum transition of hydrated electrons, enabling specific interactions with biomolecules such as viral surface proteins.

[0026] As Figure 2 shown, from an energetic perspective, the reconstructed hydrogen bond network is in a special metastable state. In this embodiment, the reduction potential is located in an exact energy window with an ORP value of -150 mV, such that the conditioning solution can precisely affect the charged groups on the virus surface without interfering with the normal functions of the cell membrane. At the kinetic level, the electron transfer process conforms to Marcus theory, and the system reaches an ideal state under optimized dilution conditions: the activation energy barrier for electron transfer is reduced while maintaining sufficient selectivity. Hydrated electrons in the diluent interact precisely with biomolecules through quantum tunneling effects. This interaction is highly selective, mainly based on the matching of molecular orbital energy levels and the optimization of electron transfer kinetics. Hydrated electrons can recognize specific molecular structures and change their functional states through the electron transfer process.

[0027] As Figure 3As shown, using its unique quantum tunneling effect, hydrated electrons can cross the energy barrier restricted by classical physics and precisely interact with specific protein structures on the virus surface. This interaction includes four key stages: initial approach (starting from this stage at time 0 ms), quantum tunneling (0.1 - 1 ms), structural change (1 - 10 ms), and final inactivation (10 - 100 ms). Thus, it can be seen that its action speed is very fast. By changing the charge distribution on the virus surface, the present invention significantly reduces the binding ability of the virus to cell receptors, thereby blocking the invasion pathway of the virus. This antiviral mechanism makes the regulating liquid highly selective and, while inactivating the virus, does not affect the physiological functions of normal cells.

[0028] As Figure 6 shown, detected by a professional quantum state analysis system, the regulating liquid prepared in this example has excellent quantum tunneling efficiency (96.3%), the electron activity retention rate reaches 98.5%, the molecular selectivity for the virus exceeds 95%, and the safety is 100%.

[0029] Example 2: Antiviral (onset period) The biological cell regulating liquid used in this example includes an electromagnetic base liquid and deionized water. The electromagnetic base liquid is the one prepared in the US 11691906B1 patent, and the mass ratio of deionized water to the electromagnetic base liquid is 2000:1; the pH value of the dilution liquid is 10, and the ORP value is -100 mV.

[0030] The virus and incubation method used in this example are the same as those in Example 1.

[0031] After incubation, virus titer determination shows that the virus infectivity decreases by more than 95%, while the normal cells in the control group are not affected.

[0032] Example 3: Antiviral (synergistic period) The biological cell regulating liquid used in this example includes an electromagnetic base liquid and deionized water. The electromagnetic base liquid is the one prepared in the US 11691906B1 patent, and the mass ratio of deionized water to the electromagnetic base liquid is 1800:1; the pH value range of the dilution liquid is 10.6, and the ORP value is -120 mV.

[0033] The virus and incubation method used in this example are the same as those in Example 1.

[0034] After incubation, virus titer determination shows that the virus infectivity decreases by more than 95.6%, while the normal cells in the control group are not affected.

[0035] Example 4: Antiviral (optimal period) The biological cell regulating solution used in this embodiment includes an electromagnetic base solution and deionized water. The electromagnetic base solution is the one prepared in accordance with the patent US 11691906B1. The mass ratio of deionized water to the electromagnetic base solution is 1400:1. The pH value of the diluting solution is 11.4, and the ORP value is -150 mV.

[0036] The virus and incubation method used in this embodiment are the same as those in Embodiment 1.

[0037] After incubation, virus titer determination shows that the virus infectivity decreases by more than 98%, while the normal cells in the control group are not affected.

[0038] Embodiment 5: Immunomodulation The biological cell regulating solution involved in this embodiment is obtained through the following method: Step 1: Under an inert atmosphere, dilute the electromagnetic base liquid with deionized water. The electromagnetic base solution is the one prepared in accordance with the patent US 11691906B1. The mass ratio of deionized water to the electromagnetic base solution is 2000:1. Step 2: Stop dilution when the detected pH value of the liquid is 10 and the ORP value is -100 mV. Step 3: After dilution is completed, let it stand for 36 hours. Detect by infrared spectroscopy that the stretching peak of hydroxyl -OH in the regulating solution is at 3275±10 cm⁻¹ to obtain the biological cell regulating solution required in this embodiment.

[0039] As Figure 4 shown, in this embodiment, spleen NK cells, T lymphocytes, and peritoneal macrophages isolated from C57BL / 6 mice are used for experiments. The cells are respectively cultured in RPMI 1640 medium containing 10% fetal bovine serum, divided into a control group and a treatment group. The treatment group is added with a regulating solution with a volume ratio of 1:20. For NK cells, hydrated electrons enhance their killing activity by 85.8% by regulating the activation of the intracellular calcium ion signaling pathway. In terms of T lymphocytes, a moderate reducing environment promotes the activation of the T cell receptor (TCR) signaling pathway, significantly enhancing their antigen response ability, and the cell proliferation ability is increased by 76.4%. For macrophages, hydrated electrons optimize their redox balance, enhancing their phagocytosis function by 92.3% and at the same time enhancing their antigen presentation ability. This multi - cell synergistic immune enhancement effect is persistent and stable, and will not cause an over - reaction of the immune system.

[0040] In this embodiment, the stability time of the regulating solution reaches 1440 minutes (24 hours), and the experimental repeatability exceeds 99%.

[0041] Embodiment 6: Anti - inflammation.

[0042] The biological cell regulating fluid involved in this embodiment is obtained through the following methods: Step 1: Under an inert atmosphere, deionized water is used to dilute the electromagnetic base liquid. The electromagnetic base liquid is the one prepared in accordance with the patent US 11691906B1. The mass ratio of deionized water to the electromagnetic base liquid is 1200:1. Step 2: When it is detected that the pH value of the liquid is 11.7 and the ORP value is -200 mV, stop the dilution. Step 3: After the dilution is completed, let it stand for 48 hours. By detecting the stretching peak of hydroxyl -OH in the regulating fluid through infrared spectroscopy at 3285±10 cm⁻¹, the biological cell regulating fluid required for this embodiment is obtained.

[0043] As Figure 5 shown, a RAW264.7 mouse macrophage inflammation model induced by LPS (lipopolysaccharide, 1 μg / mL) is used. The cells are divided into a blank control group, an LPS induction group, and a regulating fluid treatment group. In the treatment group, a regulating fluid with a volume ratio of 1:15 is added simultaneously when adding LPS. The Discovery HS Bio-Plex system is used to synchronously monitor the secretion level of cell inflammatory factors; DCFH-DA fluorescent probe and flow cytometer are used to detect the intracellular reactive oxygen species level; real-time fluorescence quantitative PCR technology is used to analyze the changes in the expression of anti-inflammatory related genes.

[0044] Experimental data shows that after 24 hours, the levels of key inflammatory factors (including TNF-α, IL-1β, etc.) in the treatment group are reduced by 82.5%. Through real-time fluorescence detection, it is found that the reactive oxygen species level in the treatment group is reduced by 89.7%, and the redox balance of the cells is optimized. Further gene expression analysis shows that there are favorable changes in the expression profiles of anti-inflammatory related genes. This anti-inflammatory effect is persistent and can still be maintained for a considerable time after stopping the drug administration, indicating that the inflammation regulation mediated by hydrated electrons has good biological effects.

[0045] Anti-inflammatory effect time curve data:

[0046] Real-time PCR results show that the expressions of anti-inflammatory genes IL-10, TGF-β, and SOCS3 in the treatment group are up-regulated by 2.8 times, 3.2 times, and 2.5 times respectively compared with the LPS group, while the expressions of pro-inflammatory genes COX-2, iNOS, and NF-κB are down-regulated by 3.5 times, 2.9 times, and 3.1 times respectively, indicating that the regulating fluid achieves deep inflammation control through multi-target actions.

[0047] Through its unique quantum state structure, hydrated electrons achieve deep-level inflammation control. This process mainly functions at three levels: firstly, directly scavenging excessive reactive oxygen species (ROS) to reduce the level of oxidative stress; secondly, optimizing the antioxidant capacity of cells by regulating the balance of redox pairs such as the intracellular glutathione system (GSH / GSSG) and nicotinamide adenine dinucleotide (NADH / NAD+); thirdly, reducing the production of key inflammatory factors, including TNF-α, IL-1β, etc., with an overall reduction of 82.5%. This multi-level anti-inflammatory effect can not only effectively control acute inflammatory responses but also prevent the occurrence of chronic inflammation.

[0048] The beneficial effects of the present invention are as follows: 1. In terms of antiviral activity, it can inactivate viruses with a rate exceeding 95%, has high selectivity, does not affect normal cells, acts rapidly, and the electron transfer rate reaches the nanosecond level. 2. In terms of immunomodulation, it can simultaneously enhance the functions of various immune cells, with a lasting and stable regulatory effect and no risk of excessive immune responses. 3. In terms of anti-inflammation, it can significantly reduce the levels of inflammatory factors, optimize the cellular redox balance without affecting normal physiological functions. 4. It has good safety guarantees, good biocompatibility, no cumulative side effects, and sufficient stability time.

Claims

1. A biological cell conditioning solution, characterized in that: It comprises electromagnetic base liquid and deionized water. The electromagnetic base liquid adopts the electromagnetic base liquid prepared in US11691906B1 patent. The mass ratio of deionized water to electromagnetic base liquid is 1000:1-2000:

1. The pH value of the diluent ranges from 10 to 12, and the ORP value ranges from -200 mV to -100 mV.

2. An antiviral liquid, characterized in that: Its therapeutic ingredients include biological cell conditioning fluid.

3. An immunomodulatory solution, characterized in that: Its therapeutic ingredients include biological cell conditioning fluid.

4. An anti-inflammatory liquid, characterized in that: Its therapeutic ingredients include biological cell conditioning fluid.

5. A method for preparing a biological cell conditioning solution, characterized in that: The following steps are involved: Step 1: Under an inert atmosphere, the electromagnetic base liquid is diluted with deionized water, wherein the electromagnetic base liquid is the electromagnetic base liquid prepared in US11691906B1, and the mass ratio of deionized water to the electromagnetic base liquid is 1000:1-2000:1; Step 2: When the pH value of the liquid is detected to be 10-12 and the ORP value is -200 mV to -100 mV, stop dilution; Step 3: After dilution, let it stand for at least 24 hours to allow the hydrogen bond network in the liquid to reorganize and form a stable long-range ordered structure.

6. The method for preparing a biological cell conditioning solution according to claim 2, characterized in that: The ORP value of the regulating solution is -150mV.

7. The method for preparing a biological cell conditioning solution according to claim 2, characterized in that: The pH value of the liquid in step 2 ranges from 11.5 to 12.

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