Reagent bottle for preventing generation and eliminating bubbles and bubble eliminating method

Abstract

The invention relates to the technical field of biochemical immune instruments, in particular to a reagent bottle capable of preventing and eliminating bubbles and a bubble eliminating method. The reagent bottle comprises a bottle body, a bottle opening, a ventilation bubble-isolating structure, a baffle and a bottle cap; the bottle body is provided with a groove, a liquid suction cavity, a liquid storage cavity, a channel and a tunnel, the bottle body is perpendicular to a table top, the bottle cap is screwed on the bottle body, then the bottle body is rotated in the clockwise direction, a reagent in the liquid suction cavity flows into the liquid storage cavity through the channel, and foam flows into the liquid storage cavity along with the reagent; the bottle body is continuously rotated in the clockwise direction and turned over, so that the reagent in the liquid storage cavity flows back into the liquid suction cavity from the ventilation bubble-isolating structure and the tunnel, bubbles gradually approach the ventilation bubble-isolating structure and the tunnel along with the liquid level of the reagent and are isolated or damaged, and therefore, the reagent in the liquid suction cavity is free of bubbles, quantitative suction of the sampling needle is ensured, and the accuracy of a test result is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of biochemical immune instruments, and particularly relates to a reagent bottle for preventing the generation and eliminating bubbles and a method for eliminating bubbles. Background Art

[0002] In the field of medical devices, biochemical analysis and diagnosis is an important means for biochemical experiments. It can automatically complete analysis and detection, obtain clinical diagnostic information by analyzing the chemical components of human samples (body fluids, tissues, etc.), and then judge diseases or body functions.

[0003] When a biochemical immune instrument performs in vitro detection, different types of reagents are required according to the detection items. Different types of reagents are filled into reagent bottles, and then placed on the special reagent bottle bracket of the instrument. Then, a sampling needle of the instrument automatically aspirates a quantitative reagent and transfers it into the cuvette of the reaction disk. After mixing and incubating with the sample, the information of the reaction solution is obtained through the principle of photoelectric colorimetry.

[0004] When the biochemical immune instrument is working, according to different test items, it will rotate the reagent bottle bracket to move the reagent bottle containing the reagent for different items to the sampling position of the sampling needle. In this way, the reagent bottle will rotate with the reagent bottle bracket. Especially for high-speed instruments, the reagent disk starts and stops frequently and rotates at a high speed, resulting in the shaking of the liquid level of the reagent in the reagent bottle, causing the sampling needle to fail to aspirate quantitatively or aspirate empty, resulting in inaccurate testing or no result, delaying the treatment of patients. In addition, the oscillation and shaking of the reagent will generate bubbles, which will also cause the sampling needle to fail to aspirate quantitatively, aspirate empty or give a false alarm, resulting in inaccurate testing or no result, delaying the treatment of patients. After bubbles are generated in the reagent, the current common practice is to use a pipette to suck away the bubbles or use a puncture tool to eliminate the bubbles. The operation is difficult, time-consuming, and the defoaming tool may contaminate the reagent. Summary of the Invention

[0005] The purpose of the present invention is to provide a reagent bottle for preventing the generation and eliminating bubbles and a method for eliminating bubbles, aiming to solve the problems that when the biochemical immune instrument is working, using a pipette to suck away the bubbles or using a puncture tool to eliminate the bubbles, the operation is difficult, time-consuming, and the defoaming tool may contaminate the reagent.

[0006] To achieve the above object, in a first aspect, the present invention provides a reagent bottle for preventing the generation and elimination of bubbles, comprising a bottle body, a bottle mouth, a ventilation and bubble separation structure, a baffle, and a bottle cap; the bottle mouth is communicated with the bottle body and is located at the top of the bottle body, the ventilation and bubble separation structure is arranged on the side of the bottle body close to the bottle mouth, the bottle cap is threadedly connected to the bottle mouth and is located on one side of the bottle mouth, the bottle body has a groove, a liquid suction cavity, a liquid storage cavity, a channel, and a tunnel, the groove is located on one side of the bottle body, the baffle is fixedly connected to the bottle body and is located in the groove, the liquid suction cavity is located on one side of the baffle, the liquid storage cavity is located on the side of the baffle away from the liquid suction cavity, the tunnel is located between the ventilation and bubble separation structure and the baffle, and the channel is located at the bottom of the baffle.

[0007] Wherein, the ventilation and bubble separation structure is higher than the bottle shoulder of the bottle body and lower than the bottle mouth.

[0008] Wherein, except for the channel at the bottom of the bottle body and the connection with the ventilation and bubble separation structure at the top, the liquid suction cavity and the liquid storage cavity are completely isolated.

[0009] Wherein, the bottle body has a chamfer.

[0010] In a second aspect, the present invention further provides a method for eliminating bubbles in a reagent bottle for preventing the generation and elimination of bubbles, which is applied to the reagent bottle for preventing the generation and elimination of bubbles as described in the first aspect above, and comprises the following steps:

[0011] Place the bottle body perpendicular to the tabletop and screw on the bottle cap, then rotate the bottle body in the clockwise direction, and let the reagent in the liquid suction cavity flow into the liquid storage cavity through the channel, and the foam flows into the liquid storage cavity together with the reagent;

[0012] Continue to rotate the bottle body in the clockwise direction, turn the bottle body over, so that the reagent in the liquid storage cavity flows back from the ventilation and bubble separation structure and the tunnel to the liquid suction cavity, and the bubbles gradually approach the ventilation and bubble separation structure and the tunnel along with the liquid level of the reagent, and are isolated or destroyed;

[0013] Rotate the bottle body in the clockwise direction again, the reagent flows from the liquid suction cavity through the channel into the liquid storage cavity. At this time, the isolated bubbles remain at the ventilation and bubble separation structure and the tunnel opening or slide to the liquid level of the reagent in the liquid storage cavity. Continue to rotate the bottle body in the clockwise direction to make the bottle body stand upright perpendicular to the tabletop.

[0014] A reagent bottle for preventing the generation and eliminating of bubbles according to the present invention. The ventilation and bubble separation structure is higher than the bottle shoulder of the bottle body and lower than the bottle mouth, maximizing the capacity of the bottle body without occupying the space above the bottle body. At the same time, it also serves as a handle for the bottle body. The groove penetrates through the bottle body. The baffles on both sides of the groove are used to separate the bottle body to form the liquid suction cavity and the liquid storage cavity. The liquid cavity and the liquid storage cavity are communicated through the channel at the bottom. The area where the reagent contacts the air is the liquid level of the liquid suction cavity. With a small area, the volatilization is slow. Only the tunnel in the liquid storage cavity communicates with the outside. Compared with direct contact with air, the volatilization speed is greatly reduced, and the expiration period after opening the whole bottle of reagent is improved. The size value of the tunnel is set according to requirements. Both gas and liquid can pass through, and bubbles can be isolated. Some bubbles are destroyed because they enter the tunnel from a large space. Therefore, after passing through the tunnel from the liquid storage cavity, the bubbles are isolated or destroyed, so that the reagent entering the liquid suction cavity has no bubbles, ensuring quantitative suction by the sampling needle and ensuring accurate test results. Specifically, place the bottle body perpendicular to the tabletop and screw on the bottle cap. Then rotate the bottle body in the clockwise direction, and the reagent in the liquid suction cavity flows into the liquid storage cavity through the channel. The foam flows into the liquid storage cavity together with the reagent. Continue to rotate the bottle body in the clockwise direction, turn the bottle body over, so that the reagent in the liquid storage cavity flows back to the liquid suction cavity from the ventilation and bubble separation structure and the tunnel. The bubbles gradually approach the ventilation and bubble separation structure and the tunnel along with the liquid level of the reagent and are isolated or destroyed, so that the reagent in the liquid suction cavity has no bubbles. Rotate the bottle body in the clockwise direction again, and the reagent flows from the liquid suction cavity into the liquid storage cavity through the channel. At this time, the isolated bubbles remain at the ventilation and bubble separation structure and the tunnel opening or slide to the liquid level of the reagent in the liquid storage cavity. Continue to rotate the bottle body in the clockwise direction to make the bottle body stand upright perpendicular to the tabletop, solving the problems that when a biochemical immune instrument is working, using a pipette to suck away bubbles or using a puncturing tool to eliminate bubbles is difficult to operate, time-consuming, and the defoaming tool may contaminate the reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0016] Figure 1 is a schematic structural diagram of a reagent bottle for preventing the generation and eliminating of bubbles provided by the present invention.

[0017] Figure 2 is an internal cross-sectional view of a reagent bottle for preventing the generation and eliminating of bubbles provided by the present invention.

[0018] Figure 3 It is a schematic diagram after the reagent is introduced into the bottle body.

[0019] Figure 4 It is a schematic diagram when the bottle body rotates 45 degrees.

[0020] Figure 5 It is a schematic diagram when the bottle body rotates to a certain angle and the reagent in the liquid suction cavity just completely enters the liquid storage cavity. This angle changes with the amount of reagent in the reagent bottle.

[0021] Figure 6 It is a schematic diagram when the bottle body rotates to a certain angle and the reagent in the liquid suction cavity completely enters the liquid storage cavity. This angle changes with the amount of reagent in the reagent bottle.

[0022] Figure 7 It is a schematic diagram when the bottle body rotates 180 degrees.

[0023] Figure 8 It is a schematic diagram when the bottle body rotates 225 degrees.

[0024] Figure 9 It is a schematic diagram when the bottle body rotates to a certain angle and the reagent in the liquid storage cavity just completely passes through the tunnel and enters the liquid suction cavity. This angle changes with the amount of reagent in the reagent bottle.

[0025] Figure 10 It is a schematic diagram when the bottle body rotates to a certain angle and the reagent in the liquid storage cavity completely passes through the tunnel and enters the liquid suction cavity. This angle changes with the amount of reagent in the reagent bottle.

[0026] Figure 11 It is a schematic diagram after the bottle body rotates back to the reset position.

[0027] Figure 12 It is a flowchart of a method for eliminating air bubbles in a reagent bottle provided by the present invention to prevent the generation and elimination of air bubbles.

[0028] In the figure: 1 - bottle body, 2 - bottle mouth, 3 - groove, 4 - ventilation and bubble separation structure, 5 - bottle shoulder, 11 - liquid suction cavity, 12 - liquid storage cavity, 13 - baffle, 14 - channel, 15 - tunnel, 21 - reagent, 22 - air bubble, 31 - bottle cap. Detailed implementation manners

[0029] The following details the embodiments of the present invention. The examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0030] Please refer to Figures 1 to 11, in a first aspect, the present invention provides a reagent bottle for preventing the generation and elimination of bubbles, comprising a bottle body 1, a bottle mouth 2, a ventilation and bubble separation structure 4, a baffle 13 and a bottle cap 31; the bottle mouth 2 is communicated with the bottle body 1 and is located at the top of the bottle body 1, the ventilation and bubble separation structure 4 is arranged on one side of the bottle body 1 close to the bottle mouth 2, the bottle cap 31 is threadedly connected with the bottle mouth 2 and is located on one side of the bottle mouth 2, the bottle body 1 has a groove 3, a liquid suction cavity 6, a liquid storage cavity 12, a channel 14 and a tunnel 15, the groove 3 is located on one side of the bottle body 1, the baffle 13 is fixedly connected with the bottle body 1 and is located in the groove 3, the liquid suction cavity 6 is located on one side of the baffle 13, the liquid storage cavity 12 is located on the side of the baffle 13 away from the liquid suction cavity 6, the tunnel 15 is located between the ventilation and bubble separation structure 4 and the baffle 13, and the channel 14 is located at the bottom of the baffle 13.

[0031] In an embodiment of the present invention, the ventilation and bubble separation structure 4 is higher than the bottle shoulder 5 of the bottle body 1 and lower than the bottle mouth 2, maximizing the capacity of the bottle body 1 without occupying the space above the bottle body 1. At the same time, it also serves as a handle for the bottle body 1. The groove 3 penetrates through the bottle body 1, and the baffles 13 on both sides of the groove 3 are used to separate the bottle body 1 to form the liquid suction cavity 6 and the liquid storage cavity 12. The liquid suction cavity 6 and the liquid storage cavity 12 are connected through the channel 14 at the bottom. The area where the reagent 21 contacts the air is the liquid level of the liquid suction cavity 6. With a small area, the volatilization is slow. Only the tunnel 15 in the liquid storage cavity 12 communicates with the outside. Compared with direct contact with air, the volatilization speed is greatly reduced, and the post-opening shelf life of the whole bottle of reagent 21 is improved. The size value of the tunnel 15 is set according to requirements. Both gas and liquid can pass through, and bubbles 22 can be isolated. Some bubbles 22 are destroyed because they enter the tunnel 15 from a large space. Therefore, after passing through the tunnel 15 from the liquid storage cavity 12, the bubbles 22 are isolated or destroyed, so that the reagent 21 entering the liquid suction cavity 6 has no bubbles 22, ensuring quantitative aspiration by the sampling needle and ensuring accurate test results. Specifically, place the bottle body 1 perpendicular to the tabletop and screw on the bottle cap 31, then rotate the bottle body 1 in the clockwise direction, and let the reagent 21 in the liquid suction cavity 6 flow into the liquid storage cavity 12 through the channel 14. The foam flows into the liquid storage cavity 12 together with the reagent 21; continue to rotate the bottle body 1 in the clockwise direction, turn the bottle body 1 over so that the reagent 21 in the liquid storage cavity 12 flows back to the liquid suction cavity 6 from the ventilation and bubble separation structure 4 and the tunnel 15. The bubbles 22 gradually approach the ventilation and bubble separation structure 4 and the tunnel 15 along with the liquid level of the reagent 21 and are isolated or destroyed, so that there are no bubbles in the reagent in the liquid suction cavity 6; rotate the bottle body 1 in the clockwise direction again, and the reagent 21 flows from the liquid suction cavity 6 through the channel 14 into the liquid storage cavity 12. At this time, the isolated bubbles 22 remain at the ventilation and bubble separation structure 4 and the mouth of the tunnel 15 or slide to the liquid level of the reagent 21 in the liquid storage cavity 12. Continue to rotate the bottle body 1 in the clockwise direction to make the bottle body 1 stand upright perpendicular to the tabletop, solving the problems that when a biochemical immunoassay instrument is working, it is difficult, time-consuming to use a pipette to suck away bubbles or a puncturing tool to eliminate bubbles, and the defoaming tool may contaminate the reagent.

[0032] Please refer to Figure 12 , Second, the present invention also provides a method for eliminating bubbles in a reagent bottle to prevent the generation and elimination of bubbles, which is applied to the reagent bottle for preventing the generation and elimination of bubbles as described in the first aspect above, and includes the following steps:

[0033] S1 Place the bottle body 1 perpendicular to the tabletop and screw on the bottle cap 31, then rotate the bottle body 1 in the clockwise direction, and let the reagent 21 in the liquid suction cavity 6 flow into the liquid storage cavity 12 through the channel 14. The foam flows into the liquid storage cavity 12 together with the reagent 21;

[0034] In an embodiment of the present invention, the bottle body 1 is screwed onto the bottle cap 31 perpendicular to the tabletop, and the bottle body 1 is rotated clockwise. As Figure 4 shown in the figure, the reagent 21 in the liquid suction cavity 6 enters the liquid storage cavity 12 through the channel 14, and the bubbles 22 in the liquid suction cavity 6 flow along with the liquid level of the reagent 21 and approach the channel 14; continue to rotate the bottle body 1. When the liquid level of the reagent 21 in the liquid suction cavity 6 reaches the channel 14 at the bottom of the bottle body 1, all the bubbles 22 gather near the channel 14, as Figure 5 shown in the figure; continue to rotate the reagent 21, and the bubbles 22 flow into the liquid storage cavity 12 along with the reagent 21, as Figure 6 shown in the figure. At this time, if there are individual bubbles 22 that do not enter the liquid storage cavity 12, the bottle body 1 can be slowly rotated back and forth in a small amplitude to allow the reagent 21 to bring the bubbles 22 near the channel 14 into the liquid storage cavity 12.

[0035] S2 Continue to rotate the bottle body 1 clockwise, turn the bottle body 1 over, so that the reagent 21 in the liquid storage cavity 12 flows back from the air vent and bubble isolation structure 4 and the tunnel 15 to the liquid suction cavity 6. The bubbles 22 gradually approach the air vent and bubble isolation structure 4 and the tunnel 15 along with the liquid level of the reagent 21, and are isolated or destroyed;

[0036] In an embodiment of the present invention, continue to rotate the bottle body 1. The reagent 21 in the liquid storage cavity 12 flows back from the air vent and bubble isolation structure 4 and the tunnel 15 to the liquid suction cavity 6, as Figure 7 shown in the figure. The bubbles 22 float on the liquid surface of the reagent 21 and move along with the liquid level; continue to rotate the bottle body 1 as Figure 8 shown in the figure. The reagent 21 gradually transfers from the liquid storage cavity 12 to the liquid suction cavity 6 through the air vent and bubble isolation structure 4 and the tunnel 15. The bubbles 22 gradually approach the entrance of the air vent and bubble isolation structure 4 and the tunnel 15 along with the liquid level of the reagent 21; continue to rotate the bottle body 1, and all the reagent 21 in the liquid storage cavity 12 is transferred from the air vent and bubble isolation structure 4 and the tunnel 15 to the liquid suction cavity 6. The bubbles 22 are isolated or destroyed at the entrance of the air vent and bubble isolation structure 4 and the tunnel 15.

[0037] S3 Rotate the bottle body 1 clockwise again. The reagent 21 flows from the liquid suction cavity 6 into the liquid storage cavity 12 through the channel 14. At this time, the isolated bubbles 22 remain at the air vent and bubble isolation structure 4 and the tunnel 15 opening or slide to the liquid surface of the reagent 21 in the liquid storage cavity 12. Continue to rotate the bottle body 1 clockwise to make the bottle body 1 stand upright perpendicular to the tabletop.

[0038] In an embodiment of the present invention, as Figure 10Continue to rotate the bottle body 1 as shown. The reagent 21 flows from the liquid suction cavity 6 into the liquid storage cavity 12 through the channel 14 at the bottom of the bottle body 1. At this time, the isolated air bubbles 22 remain in the air ventilation and bubble isolation structure 4 and the mouth of the tunnel 15 or slide down to the liquid level of the reagent 21 in the liquid storage cavity 12. Continue to rotate the bottle body 1 to make the bottle body 1 stand upright and perpendicular to the desktop. At this time, there are no air bubbles 22 in the reagent 21 in the liquid suction cavity 6, which can ensure that the sampling needle sampling is not interfered.

[0039] This method combines the air ventilation and bubble isolation structure 4 and the tunnel 15 of the reagent bottle to quickly transfer the air bubbles 22 in the liquid suction cavity 6 to the liquid storage cavity 12 or destroy the air bubbles 22, so as to achieve the purpose that there are no air bubbles 22 in the reagent 21 in the liquid suction cavity 6. It is fast, convenient and does not require tools. There is no risk of contamination of the reagent 21, saving time and reagent 21.

[0040] The above-disclosed is only a preferred embodiment of a reagent bottle and a bubble elimination method for preventing the generation and eliminating air bubbles of the present invention. Of course, the scope of the rights of the present invention cannot be limited thereby. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present invention still fall within the scope covered by the invention.

Claims

1. A reagent bottle for preventing and eliminating bubbles, It is characterized by: It includes a bottle body, a bottle mouth, a ventilation bubble structure, a baffle and a bottle cap; The bottle mouth is communicated with the bottle body and is located at the top of the bottle body, the ventilation bubble structure is arranged on the side of the bottle body close to the bottle mouth, the bottle cap is threadedly connected to the bottle mouth and is located on the side of the bottle mouth, the bottle body has a groove, a liquid suction cavity, a liquid storage cavity, a channel and a tunnel, the groove is located on one side of the bottle body, the baffle is fixedly connected to the bottle body and is located in the groove, the liquid suction cavity is located on one side of the baffle, the liquid storage cavity is located on the side of the baffle away from the liquid suction cavity, the tunnel is located between the ventilation bubble structure and the baffle, and the channel is located at the bottom of the baffle.

2. The reagent bottle for preventing and eliminating bubbles as claimed in claim 1, characterized in that ； The ventilation bubble structure is higher than the bottle shoulder of the bottle body and lower than the bottle mouth.

3. The reagent bottle for preventing and eliminating bubbles as claimed in claim 1, characterized in that ； The liquid suction chamber and the liquid storage chamber are completely isolated from each other except that the channel at the bottom of the bottle body and the ventilation bubble structure at the top are connected.

4. The reagent bottle for preventing and eliminating bubbles as claimed in claim 1, characterized in that ； The bottle body has chamfers.

5. A method for eliminating bubbles in a reagent bottle for preventing and eliminating bubbles, applied to the reagent bottle for preventing and eliminating bubbles as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: Place the bottle vertically on the table and screw on the bottle cap, then rotate the bottle clockwise to allow the reagent in the aspiration chamber to flow into the liquid storage chamber through the channel, and the foam flows into the liquid storage chamber along with the reagent; Continue to rotate the bottle body in a clockwise direction and flip the bottle body over, so that the reagent in the liquid storage chamber flows back from the ventilation bubble structure and the tunnel into the liquid aspiration chamber, and the bubbles gradually move toward the ventilation bubble structure and the tunnel along with the reagent liquid level and are isolated or destroyed; The bottle body is rotated clockwise again, and the reagent flows from the aspiration chamber through the channel into the liquid storage chamber. At this time, the isolated bubbles remain in the ventilation bubble structure and the tunnel mouth or slide to the reagent liquid surface of the liquid storage chamber. The bottle body is continued to be rotated clockwise to make the bottle body stand upright and perpendicular to the table.

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