Separation equipment for mouse primary hepatocytes
By designing a murine primary hepatocyte separation device containing a separation cavity, a separation mechanism and a rotating electric machine, the problems of low cell separation efficiency and instability of the test tube in the prior art are solved, and efficient and stable separation of multiple sets of test tube cells is achieved.
Patent Information
- Application Number
- CN202510164596.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-14
- Publication Date
- 2025-05-30
AI Technical Summary
In the prior art, cell separation efficiency is low, multiple sets of test tubes cannot be processed simultaneously, and the test tubes are unstable, which easily leads to incomplete separation and contamination of test tube cells.
A separation device for primary mouse hepatocytes is designed, including a separation cavity, a separation mechanism, a group of test tube mouths, a test tube groove, a mounting rod, a spring, a clamping block, a drive shaft and a rotating motor. The separation mechanism is driven by the rotating motor, and the cell separation is separated by centrifugal force, and the up and down movement of the separation mechanism is realized through the lifting assembly and the lifting motor.
It improves the efficiency and stability of cell isolation, and can handle multiple sets of test tubes simultaneously, ensuring thorough separation and reducing contamination of test tube cells.
Smart Images
Figure CN120059889A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of cell separation equipment, and specifically to a separation equipment for murine primary hepatocytes. Background Art
[0002] The separation of murine primary hepatocytes is mainly based on the principles of cell biology and biochemistry. During the separation process, specific enzymes are required to digest liver tissues to release hepatocytes from the liver tissues. At the same time, mechanical methods are also needed to assist in the separation of hepatocytes. After the separation of hepatocytes, further purification and separation are required to obtain high-quality hepatocytes. Modern cell separation and purification equipment usually adopts techniques such as centrifugation, filtration, and gradient centrifugation, which can effectively remove impurities and dead cells and improve the purity and activity of hepatocytes.
[0003] The following defects still exist in the prior art during use:
[0004] During the separation of cells in the prior art, the separation is usually carried out by manually shaking test tubes or operating a single set of equipment. The overall separation efficiency is low, and multiple sets of test tubes cannot be separated simultaneously. The overall separation is incomplete, and the test tubes are unstable, which easily causes incomplete separation and contamination of the test tube cells.
[0005] In view of this, we propose a separation equipment for murine primary hepatocytes to solve the existing problems. Summary of the Invention
[0006] The purpose of the present invention is to provide a separation equipment for murine primary hepatocytes to solve the problems raised in the above background art.
[0007] To achieve the above purpose, the present invention provides the following technical solution: A separation equipment for murine primary hepatocytes, including a separation equipment. A separation chamber is provided inside the separation equipment. A separation mechanism is installed in the separation chamber. Multiple test tube openings are arranged around the separation mechanism. A test tube slot is provided at the lower end of the test tube opening. Installation rods are installed on both sides of the bottom of the test tube slot. Springs are installed on the installation rods. The installation rods penetrate through the inner wall of the test tube slot and are provided with clamping blocks. A drive shaft is installed at the center of the separation mechanism. A rotary motor is installed at the lower end of the drive shaft.
[0008] Preferably, a lifting component is installed at the lower end of the rotary motor inside the separation chamber, and a lifting motor is installed at the lower end of the lifting component.
[0009] Preferably, a temperature control chip is provided inside the separation chamber, and a temperature control sensor is installed on the separation equipment near the separation chamber.
[0010] Preferably, a control console is installed on one side of the separation device, and the control console is electrically connected to a temperature control sensor, a rotating motor, and a lifting motor.
[0011] Preferably, mounting plates are installed on both sides of the separation chamber. Slots are formed in the mounting plates, and push rods are installed in the slots. The push rods are connected to movable cover plates inside the mounting plates.
[0012] Preferably, a plurality of bottom support feet are installed at the bottom of the separation device.
[0013] Compared with the prior art, the beneficial effects of the present invention are as follows:
[0014] The present invention designs a separation device. A separation mechanism dedicated to separation is provided in the separation device. Multiple test tubes can be installed through the separation mechanism. And through the spring abutting member at the bottom of the separation mechanism, the bottom of the test tube is stably fixed in the test tube slot. Then, the rotation of the separation mechanism is controlled by the rotating motor, and separation is carried out through the action of centrifugal force. The overall efficiency is relatively high and the stability is also relatively high. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 is a three-dimensional structure schematic diagram of the present invention;
[0016] Figure 2 is a top view structure schematic diagram of the present invention;
[0017] Figure 3 is a structure schematic diagram of the separation mechanism of the present invention.
[0018] In the figure: 1. Separation device; 2. Control console; 3. Separation mechanism; 4. Separation chamber; 5. Temperature control sensor; 6. Slot; 7. Mounting plate; 8. Push rod; 9. Bottom support foot; 10. Test tube slot; 11. Clamping block; 12. Spring; 13. Mounting rod; 14. Drive shaft; 15. Test tube mouth; 16. Rotating motor; 17. Lifting assembly; 18. Lifting motor. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0019] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0020] Embodiment 1
[0021] As Figure 1 , Figure 2 and Figure 3As shown in the figure, an isolation device for murine primary hepatocytes proposed by the present invention includes an isolation device 1. Inside the isolation device 1, there is an isolation chamber 4, which is a chamber used for cell isolation. An isolation mechanism 3 is installed in the isolation chamber 4. The isolation mechanism 3 is the main mechanism for separating cells. There are multiple sets of test tube openings 15 arranged around the isolation mechanism 3, and the multiple sets of test tube openings 15 are used to install test tubes. At the lower end of the test tube opening 15, there is a test tube groove 10, which is used to fix the position of the test tube. On both sides of the bottom of the test tube groove 10, there are installation rods 13. Springs 12 are installed on the installation rods 13. The installation rods 13 penetrate through the inner wall of the test tube groove 10 and are provided with clamping blocks 11. When the test tube is inserted to the bottom of the test tube groove 10, the clamping blocks 11 retract, and the springs 12 elastically control the clamping blocks 11 to clamp and fix the bottom of the test tube. At the center of the isolation mechanism 3, there is a driving shaft 14. A rotating motor 16 is installed at the lower end of the driving shaft 14. The rotating motor 16 controls the rotation of the driving shaft 14, thereby driving the isolation mechanism 3 to rotate.
[0022] Furthermore, at the lower end of the rotating motor 16 inside the isolation chamber 4, there is a lifting component 17. A lifting motor 18 is installed at the lower end of the lifting component 17. The lifting motor 18 is used to control the movement of the lifting component 17, thereby driving the isolation mechanism 3 to move up and down.
[0023] Furthermore, a temperature control chip is arranged inside the isolation chamber 4. A temperature control sensor 5 is installed on the isolation device 1 near the isolation chamber 4. The temperature control chip constantly senses the temperature inside the isolation chamber 4 and reflects it through the temperature control sensor 5.
[0024] Furthermore, a control console 2 is installed on one side of the isolation device 1. The control console 2 is electrically connected to the temperature control sensor 5, the rotating motor 16, and the lifting motor 18. The control console 2 is used to control the rotating motor 16 and the lifting motor 18 to work.
[0025] Furthermore, mounting plates 7 are installed on both sides of the isolation chamber 4. Slots 6 are opened on the mounting plates 7. Push rods 8 are installed on the slots 6. The push rods 8 are connected to a movable cover plate inside the mounting plates 7. By moving the push rods 8, the movable cover plate is driven, thereby realizing the opening and closing of the upper layer of the isolation chamber 4.
[0026] Furthermore, multiple sets of bottom support feet 9 are installed at the bottom of the isolation device 1. The multiple sets of bottom support feet 9 are used to stabilize the overall isolation device 1.
[0027] The above specific embodiments are merely several preferred embodiments of the present invention. Based on the technical solution of the present invention and the relevant revelations of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.
Claims
1. A separation device for mouse primary hepatocytes, comprising a separation device (1), characterized in that: The separation device (1) is provided with a separation chamber (4), a separation mechanism (3) is installed in the separation chamber (4), a plurality of test tube ports (15) are arranged around the separation mechanism (3), a test tube slot (10) is arranged at the lower end of the test tube port (15), mounting rods (13) are installed on both sides of the bottom of the test tube slot (10), a spring (12) is installed on the mounting rod (13), a clamping block (11) is arranged on the mounting rod (13) passing through the inner wall of the test tube slot (10), a driving shaft (14) is installed at the center of the separation mechanism (3), and a rotating motor (16) is installed at the lower end of the driving shaft (14).
2. The device for separating primary mouse hepatocytes according to claim 1, characterized in that: A lifting assembly (17) is installed at the lower end of the rotating motor (16) inside the separation chamber (4), and a lifting motor (18) is installed at the lower end of the lifting assembly (17).
3. The separation device for primary mouse hepatocytes according to claim 2, characterized in that: A temperature control chip is arranged inside the separation chamber (4), and a temperature control sensor (5) is installed on the separation device (1) near the separation chamber (4).
4. The device for separating primary mouse hepatocytes according to claim 3, characterized in that: A control console (2) is installed on one side of the separation device (1), and the control console (2) is electrically connected to a temperature control sensor (5), a rotating motor (16), and a lifting motor (18).
5. The device for separating primary mouse hepatocytes according to claim 1, characterized in that: A mounting plate (7) is installed on both sides of the separation chamber (4), a slot (6) is provided on the mounting plate (7), a push rod (8) is installed on the slot (6), and the push rod (8) is connected to a movable cover plate inside the mounting plate (7).
6. The device for separating primary mouse hepatocytes according to claim 1, characterized in that: The bottom of the separation device (1) is equipped with multiple sets of bottom supporting feet (9).