Trace DNA extraction workstation

Abstract

The invention discloses a trace DNA (deoxyribonucleic acid) extraction workstation which comprises a workbench, a plurality of mounting holes are formed in the workbench, and an extraction tank, an elution tank, a centrifugal tank and a cracking tank are respectively arranged in the plurality of mounting holes; a vertical frame is fixedly connected to the top end of the workbench, a sliding rail is fixedly connected to the vertical frame, a walking sliding block is slidably connected to the sliding rail, a liquid taking assembly is fixedly connected to the walking sliding block, and the liquid taking assembly is used for achieving liquid transfer between the grooves; a centrifugal assembly is mounted in the centrifugal tank, and a magnetic attraction device is mounted on the outer wall of the bottom of the elution tank and used for magnetically attracting and fixing magnetic beads in the elution tank; a controller is arranged on the workbench, and the walking sliding block, the centrifugal assembly and the magnetic attraction device are all electrically connected with the controller. The device is simple in structure, low in configuration cost and low in requirement for the quality of experimenters, full-automatic operation can be achieved, normal DNA extraction is guaranteed, and meanwhile the DNA extraction difficulty is lowered.

Description

Technical Field

[0001] The present invention relates to the technical field of DNA extraction, and particularly relates to a micro DNA extraction workstation. Background Art

[0002] In the fields of biological research and medicine, it is often necessary to extract the DNA of samples and then carry out subsequent research and detection work.

[0003] In the prior art, usually, experimental personnel put the sample into a lysis plate, then add lysis solution, centrifuge after the sample is lysed, and then add the lysed liquid into a binding plate to carry out DNA extraction operations. The whole DNA extraction process is very complicated, with high requirements for the quality of experimental personnel, and any carelessness may cause extraction failure.

[0004] Therefore, there is an urgent need to provide a micro DNA extraction workstation to reduce the difficulty of DNA extraction. Summary of the Invention

[0005] The purpose of the present invention is to provide a micro DNA extraction workstation to solve the problems existing in the above prior art.

[0006] To achieve the above purpose, the present invention provides a micro DNA extraction workstation, including a workbench. A plurality of mounting holes are provided in the workbench, and an extraction tank, an elution tank, a centrifugation tank, and a lysis tank are respectively arranged in the plurality of mounting holes; a vertical frame is fixedly connected to the top end of the workbench, a slide rail is fixedly connected to the vertical frame, a walking slider is slidably connected to the slide rail, and a liquid taking assembly is fixedly connected to the walking slider. The liquid taking assembly is used to realize the liquid transfer between the tanks; a centrifugation assembly is installed in the centrifugation tank, and the centrifugation assembly is used to separate the sample residue and the lysis product; a magnetic attraction device is installed on the bottom outer wall of the elution tank, and the magnetic attraction device is used to magnetically fix the magnetic beads in the elution tank; a controller is arranged on the workbench, and the walking slider, the centrifugation assembly, and the magnetic attraction device are all electrically connected to the controller.

[0007] Preferably, the liquid taking assembly includes a connecting rod fixedly connected to the walking slider. The end of the connecting rod is fixedly connected with a connecting sleeve, and a first telescopic cylinder is fixedly sleeved inside the connecting sleeve. The bottom end of the first telescopic cylinder is fixedly connected with a mounting plate, a piston and a second telescopic cylinder are fixedly installed at the bottom end of the mounting plate. A sampling cylinder is slidably sleeved outside the piston. A sampling port is arranged at the bottom end of the sampling cylinder, and a connecting plate is fixedly connected to the top side wall of the sampling cylinder. The bottom end of the second telescopic cylinder is fixedly connected with the connecting plate; the first telescopic cylinder and the second telescopic cylinder are both electrically connected to the controller.

[0008] Preferably, a sealing member is provided on the inner wall of the sampling port, and the sealing member is used to seal the sampling port.

[0009] Preferably, the sealing member includes three silicone membrane flaps, and the three silicone membrane flaps are arranged circumferentially, and two adjacent silicone membrane flaps are in contact with each other.

[0010] Preferably, an eluent storage tank, a washing liquid storage tank, a lysis liquid storage tank and a sample tank are provided at the top of the vertical frame. The lysis liquid storage tank and the sample tank are communicated with the lysis tank through a pipeline device, and the eluent storage tank and the washing liquid storage tank are communicated with the elution tank through a pipeline device; the pipeline device is electrically connected to the controller.

[0011] Preferably, the pipeline device includes a liquid outlet pipe fixedly installed on the vertical frame. Two branch pipes are fixedly communicated with the top end of the liquid outlet pipe. Pumps are installed at the ends of the two branch pipes. The inlet end of the pump is communicated with an inlet pipe, and the pump is electrically connected to the controller.

[0012] Preferably, a water tank and a waste liquid tank are installed on the workbench, and the water tank and the waste liquid tank are arranged on one side of the lysis tank away from the centrifugation tank.

[0013] Preferably, the centrifugation assembly includes a motor fixedly installed at the inner bottom of the centrifugation tank. A clamping groove is fixedly connected to the output shaft of the motor. A centrifugation cylinder is arranged in the clamping groove. A plurality of positioning blocks are fixedly connected to the inner wall of the centrifugation tank at equal intervals in the circumferential direction, and the centrifugation cylinder is slidably matched with the positioning blocks.

[0014] Preferably, a plurality of installation grooves are opened in the inner wall of the clamping groove at equal intervals in the circumferential direction. A telescopic rod is fixedly connected to the installation groove in the horizontal direction. An abutting head is fixedly connected to the end of the telescopic rod, and the abutting head abuts against the centrifugation cylinder; a spring is sleeved on the telescopic rod, and two ends of the spring respectively abut against the abutting head and the bottom of the installation groove.

[0015] Preferably, the abutting head is of an elastic arc structure, and an anti-slip pad is arranged on one side of the abutting head away from the telescopic rod.

[0016] Compared with the prior art, the present invention has the following advantages and technical effects:

[0017] The micro DNA extraction workstation provided by the present invention has a simple structure, a low configuration cost, a low requirement for the quality of experimental personnel, can realize fully automatic operation, ensure the normal extraction of DNA, and reduce the difficulty of DNA extraction. Description of the Drawings

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required for use in the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on these drawings.

[0019] Figure 1 Structural schematic diagram of the micro DNA extraction workstation of the present invention;

[0020] Figure 2 Structural schematic diagram of another perspective of the micro DNA extraction workstation of the present invention;

[0021] Figure 3 For the present invention Figure 1 Partial enlarged view of A in;

[0022] Figure 4 Structural schematic diagram of the sealing member of the present invention;

[0023] Figure 5 Connection schematic diagram of the centrifuge tube and the card slot of the present invention;

[0024] In the figure: 1, workbench; 2, extraction tank; 3, elution tank; 4, centrifugation tank; 5, lysis tank; 6, vertical frame; 7, slide rail; 8, walking slider; 9, magnetic attraction device; 10, connecting rod; 11, connecting sleeve; 12, first telescopic cylinder; 13, mounting plate; 14, piston; 15, second telescopic cylinder; 16, sampling tube; 17, connecting plate; 18, silicone membrane flap; 19, eluent storage tank; 20, washing solution storage tank; 21, lysis solution storage tank; 22, sample tank; 23, liquid outlet pipe; 24, branch pipe; 25, pump; 26, liquid inlet pipe; 27, water tank; 28, waste liquid tank; 29, motor; 30, card slot; 31, centrifuge tube; 32, positioning block; 33, telescopic rod; 34, abutting head; 35, spring. Detailed implementation manners

[0025] It should be noted that, without conflict, the embodiments in the present invention and the features in the embodiments can be combined with each other. The described embodiments are only some embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. The following will refer to the accompanying drawings and combine with the embodiments to detail the present invention.

[0026] Such as Figures 1 to 5As shown in the figure, the present invention provides a micro DNA extraction workstation, including a workbench 1, on which a plurality of mounting holes are provided. An extraction tank 2, an elution tank 3, a centrifugation tank 4 and a lysis tank 5 are respectively arranged in the plurality of mounting holes; a vertical frame 6 is fixedly connected to the top end of the workbench 1, a slide rail 7 is fixedly connected to the vertical frame 6, a walking slider 8 is slidably connected to the slide rail 7, and a liquid taking assembly is fixedly connected to the walking slider 8, and the liquid taking assembly is used to realize the liquid transfer between the tanks; a centrifugation assembly is installed in the centrifugation tank 4, and the centrifugation assembly is used to separate the sample residue and the lysis product; a magnetic attraction device 9 is installed on the bottom outer wall of the elution tank 3, the magnetic attraction device 9 adopts an electromagnet, and the magnetic attraction device 9 is used to magnetically fix the magnetic beads in the elution tank 3; a controller is arranged on the workbench 1, and the walking slider 8, the centrifugation assembly and the magnetic attraction device 9 are all electrically connected to the controller.

[0027] When the present invention is in use, first, a sample is added to the lysis tank 5, and a lysis solution is added. After the lysis is completed at the end of the set time, the controller controls the movement of the walking slider 8, and uses the liquid taking assembly to transfer the liquid in the lysis tank 5 to the centrifugation assembly in the centrifugation tank 4. The centrifugation assembly separates different products in the lysed sample in the lysis tank 5 under the action of centrifugation. After the separation is completed, the useful liquid part is transferred to the elution tank 3 again by using the liquid taking assembly, and a binding solution and magnetic beads are added to the elution tank 3. At the same time, the magnetic attraction device 9 is started to make the magnetic beads attracted to the magnetic attraction device 9. Then, the binding waste liquid in the elution tank 3 is removed by using the liquid taking assembly, a washing solution is added to the elution tank 3 to elute the non-DNA products outside the magnetic beads. After the liquid for washing the magnetic beads in the elution tank 3 is sucked out, an elution solution is added to the elution tank 3 to separate the DNA from the magnetic beads, and then the liquid containing DNA is sucked out and transferred to the extraction tank 2 to complete the DNA extraction work.

[0028] The structure of the present invention is simple, the configuration cost is low, the quality requirements for experimental personnel are low, and it can realize fully automatic operation, ensuring the normal extraction of DNA while reducing the difficulty of DNA extraction.

[0029] In a further optimized solution, the liquid taking assembly includes a connecting rod 10 fixedly connected to the walking slider 8. A connecting sleeve 11 is fixedly connected to the end of the connecting rod 10. A first telescopic cylinder 12 is fixedly sleeved inside the connecting sleeve 11. The bottom end of the first telescopic cylinder 12 is fixedly connected to a mounting plate 13. A piston 14 and a second telescopic cylinder 15 are fixedly installed at the bottom end of the mounting plate 13. A sampling cylinder 16 is slidably sleeved outside the piston 14. A sampling port is arranged at the bottom end of the sampling cylinder 16. A connecting plate 17 is fixedly connected to the top side wall of the sampling cylinder 16. The bottom end of the second telescopic cylinder 15 is fixedly connected to the connecting plate 17; both the first telescopic cylinder 12 and the second telescopic cylinder 15 are electrically connected to the controller.

[0030] In the initial state, both the first telescopic cylinder 12 and the second telescopic cylinder 15 are in the shortened state. During use, the sampling cylinder 16 is inserted into the corresponding groove through the first telescopic cylinder 12. Then, the second telescopic cylinder 15 is controlled to extend, and at the same time, the first telescopic cylinder 12 is controlled to shorten. The extended length is kept consistent with the shortened length, so that the sampling cylinder 16 slides relative to the piston 14, and the relative position of the sampling cylinder 16 remains unchanged, thereby sucking the liquid in the groove into the sampling cylinder 16.

[0031] In a further optimized solution, to prevent the liquid in the sampling cylinder 16 from dripping during liquid transfer, a sealing member is provided on the inner wall of the sampling port, and the sealing member is used to seal the sampling port.

[0032] In a further optimized solution, to not affect the liquid from entering and leaving the sampling cylinder 16, the sealing member includes three silicone membrane flaps 18. The three silicone membrane flaps 18 are arranged circumferentially, and two adjacent silicone membrane flaps 18 are in contact with each other.

[0033] In a further optimized solution, to facilitate the supply of samples, lysis solutions, washing solutions, and elution solutions, an elution solution storage tank 19, a washing solution storage tank 20, a lysis solution storage tank 21, and a sample tank 22 are provided at the top of the vertical frame 6. The lysis solution storage tank 21 and the sample tank 22 are connected to the lysis tank 5 through a pipeline device, and the elution solution storage tank 19 and the washing solution storage tank 20 are connected to the elution tank 3 through a pipeline device; the pipeline device is electrically connected to the controller.

[0034] In a further optimized solution, to be able to achieve continuous DNA extraction work, the pipeline device includes a liquid outlet pipe 23 fixedly installed on the vertical frame 6. The top of the liquid outlet pipe 23 is fixedly connected to two branch pipes 24. Pumps 25 are installed at the ends of the two branch pipes 24. The inlet end of the pump 25 is connected to an inlet pipe 26, and the pump 25 is electrically connected to the controller.

[0035] In a further optimized solution, to facilitate the timely cleaning of the sampling cylinder 16 and avoid cross - contamination between liquids, a water tank 27 and a waste liquid tank 28 are installed on the workbench 1. The water tank 27 and the waste liquid tank 28 are arranged on the side of the lysis tank 5 away from the centrifugation tank 4.

[0036] In a further optimized solution, to facilitate the centrifugal separation of the lysed samples and maintain the stable operation of the centrifugation assembly, the centrifugation assembly includes a motor 29 fixedly installed at the inner bottom of the centrifugation tank 4. A clamping groove 30 is fixedly connected to the output shaft of the motor 29. A centrifugal cylinder 31 is arranged in the clamping groove 30. A plurality of positioning blocks 32 are fixedly connected to the inner wall of the centrifugation tank 4 at equal intervals circumferentially, and the centrifugal cylinder 31 is in sliding fit with the positioning blocks 32.

[0037] For a further optimized solution, to ensure an effective connection between the centrifugal cylinder 31 and the card slot 30 and prevent the motor 29 from idling, a plurality of mounting grooves are circumferentially and equidistantly formed along the inner wall of the card slot 30. A telescopic rod 33 is fixedly connected horizontally in the mounting groove. The end of the telescopic rod 33 is fixedly connected with an abutting head 34, and the abutting head 34 abuts against the centrifugal cylinder 31. A spring 35 is sleeved on the telescopic rod 33, and the two ends of the spring 35 respectively abut against the abutting head 34 and the bottom of the mounting groove.

[0038] When the centrifugal cylinder 31 is not installed, the spring 35 remains in a normal state, the abutting head 34 remains in a state of protruding from the mounting groove, and the edge position of the abutting head 34 is still located within the mounting groove. When the centrifugal cylinder 31 is placed into the card slot 30, the setting of the abutting head 34 will not affect the insertion of the centrifugal cylinder 31. After the centrifugal cylinder 31 is inserted, the spring 35 is in a compressed state, so that a pressing state is formed between the abutting head 34 and the centrifugal cylinder 31, realizing the fixation of the centrifugal cylinder 31.

[0039] For a further optimized solution, to facilitate the installation of the centrifugal cylinder 31 into the card slot 30 and increase the friction between the abutting head 34 and the centrifugal cylinder 31, the abutting head 34 is of an elastic arc structure, and an anti-slip pad is provided on the side of the abutting head 34 away from the telescopic rod 33.

[0040] The working principle of the micro DNA extraction workstation provided by the present invention is as follows: First, the sample, lysis solution, washing solution and elution solution are sequentially added into the sample tank 22, lysis solution storage tank 21, washing solution storage tank 20 and elution solution storage tank 19, and magnetic beads and binding solution are added into the elution tank 3.

[0041] Then, the controller controls the corresponding pump 25 to work, adds the sample to the lysis tank 5, and adds the lysis solution. After the set time is over and the lysis is completed, the controller controls the walking slider 8 to move, and uses the sampling cylinder 16 to transfer the liquid in the lysis tank 5 to the centrifugal cylinder 31. The motor 29 drives the card slot 30 and the centrifugal cylinder 31 to rotate, and separates the different products in the lysed sample in the lysis tank 5 under the action of centrifugation. After the separation is completed, the sampling cylinder 16 is used again to transfer the useful liquid part to the elution tank 3, so that the DNA is combined with the magnetic beads, and the magnetic suction device is started. 9 is set to make the magnetic beads and the magnetic suction device 9 attract each other, and the combined waste liquid in the elution tank 3 is removed by using the sampling tube 16. Then, the corresponding pump 25 is controlled by the controller again to work, and the washing liquid is added to the elution tank 3 to elute the non-DNA products on the outside of the magnetic beads. After the liquid after washing the magnetic beads in the elution tank 3 is sucked out, the corresponding pump 25 is controlled by the controller to work, and the elution liquid is added to the elution tank 3 to separate the DNA from the magnetic beads. Then, the liquid containing the DNA is sucked out by using the sampling tube 16 and transferred to the extraction tank 2 to complete the DNA extraction. During the process, the sampling tube 16 needs to be cleaned with clean water in the clean water tank 27 after each use, and the cleaned water is discharged into the waste liquid tank 28. Before the sampling tube 16 transfers the liquid in the centrifugal tube 31 to the elution tank 3, the sampling tube 16 is first moved to the clean water tank 27 to clean the outer wall of the sampling tube 16. The silicone membrane petal 18 arranged at the sampling port of the sampling tube 16 can prevent water from entering the sampling tube 16, and can also prevent the liquid in the sampling tube 16 from dripping.

[0042] The present invention has a simple structure, low configuration cost, and low requirements on the quality of the experimenter. It only needs to add the binding liquid and magnetic beads to the elution tank 3 in advance to realize the full-automatic operation, thereby ensuring the normal extraction of DNA and reducing the difficulty of DNA extraction.

[0043] The above are only preferred specific implementations of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by any technician familiar with the technical field within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

Claims

1. A micro DNA extraction workstation, characterized in that: The invention comprises a workbench (1), wherein a plurality of mounting holes are provided on the workbench (1), and an extraction tank (2), an elution tank (3), a centrifugal tank (4) and a lysis tank (5) are respectively arranged in the plurality of mounting holes; a stand (6) is fixedly connected to the top of the workbench (1), a slide rail (7) is fixedly connected to the stand (6), a travel slider (8) is slidably connected to the slide rail (7), a liquid collection component is fixedly connected to the travel slider (8), and the liquid collection component is used to realize liquid transfer between tanks; a centrifugal component is installed in the centrifugal tank (4), and the centrifugal component is used to separate sample residues from lysis products; a magnetic suction device (9) is installed on the outer wall of the bottom of the elution tank (3), and the magnetic suction device (9) is used to magnetically fix the magnetic beads in the elution tank (3); a controller is arranged on the workbench (1), and the travel slider (8), the centrifugal component and the magnetic suction device (9) are all electrically connected to the controller.

2. The micro DNA extraction workstation according to claim 1, characterized in that: The liquid collection assembly comprises a connecting rod (10) fixedly connected to the walking slider (8), the end of the connecting rod (10) is fixedly connected to a connecting sleeve (11), a telescopic cylinder (12) is fixedly provided on the inner side of the connecting sleeve (11), the bottom end of the telescopic cylinder (12) is fixedly connected to a mounting plate (13), a piston (14) and a telescopic cylinder (15) are fixedly installed on the bottom end of the mounting plate (13), a sampling cylinder (16) is provided on the outer sliding sleeve of the piston (14), a sampling port is provided at the bottom end of the sampling cylinder (16), a connecting plate (17) is fixedly connected to the top side wall of the sampling cylinder (16), and the bottom end of the telescopic cylinder (15) is fixedly connected to the connecting plate (17); the telescopic cylinder (12) and the telescopic cylinder (15) are both electrically connected to the controller.

3. The micro DNA extraction workstation according to claim 2, characterized in that: A sealing member is arranged on the inner wall of the sampling port, and the sealing member is used to seal the sampling port.

4. The micro-DNA extraction workstation according to claim 3, characterized in that: The sealing member comprises three silicone membrane petals (18), the three silicone membrane petals (18) are arranged along the circumferential direction, and two adjacent silicone membrane petals (18) are fitted together.

5. The micro DNA extraction workstation according to claim 1, characterized in that: The top of the stand (6) is provided with an eluent storage tank (19), a washing liquid storage tank (20), a lysis liquid storage tank (21) and a sample tank (22); the lysis liquid storage tank (21) and the sample tank (22) are connected to the lysis tank (5) via a pipeline device; the eluent storage tank (19) and the washing liquid storage tank (20) are connected to the elution tank (3) via a pipeline device; the pipeline device is electrically connected to the controller.

6. The micro DNA extraction workstation according to claim 5, characterized in that: The pipeline device comprises a liquid outlet pipe (23) fixedly mounted on the stand (6); the top end of the liquid outlet pipe (23) is fixedly connected to two branch pipes (24); the ends of the two branch pipes (24) are both equipped with pumps (25); the liquid inlet end of the pump (25) is connected to a liquid inlet pipe (26); and the pump (25) is electrically connected to the controller.

7. The micro DNA extraction workstation according to claim 1, characterized in that: A clean water tank (27) and a waste liquid tank (28) are installed on the workbench (1), and the clean water tank (27) and the waste liquid tank (28) are arranged on a side of the lysis tank (5) away from the centrifugal tank (4).

8. The micro DNA extraction workstation according to claim 1, characterized in that: The centrifugal assembly comprises a motor (29) fixedly mounted on the inner bottom of the centrifugal tank (4); a clamping groove (30) is fixedly connected to the output shaft of the motor (29); a centrifugal cylinder (31) is arranged in the clamping groove (30); a plurality of positioning blocks (32) are fixedly connected to the inner wall of the centrifugal tank (4) at equal intervals in the circumferential direction; the centrifugal cylinder (31) is slidably matched with the positioning blocks (32).

9. The micro DNA extraction workstation according to claim 8, characterized in that: A plurality of mounting grooves are formed on the inner wall of the clamping groove (30) at equal intervals in the circumferential direction, a telescopic rod (33) is fixedly connected in the mounting groove in the horizontal direction, an abutment joint (34) is fixedly connected to the end of the telescopic rod (33), and the abutment joint (34) abuts against the centrifugal cylinder (31); a spring (35) is sleeved on the telescopic rod (33), and two ends of the spring (35) abut against the abutment joint (34) and the bottom of the mounting groove, respectively.

10. The micro DNA extraction workstation according to claim 9, characterized in that: The abutment joint (34) is an elastic arc-shaped structure, and a non-slip pad is provided on a side of the abutment joint (34) away from the telescopic rod (33).

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