Bioreactor

Abstract

The bioreactor comprises a reaction tank, the reaction tank is provided with a reaction cavity and a mounting position arranged at the bottom of the reaction tank, and the mounting position is provided with a first avoiding opening; the lifting assembly comprises a lifting piece, and the lifting piece is arranged on the side, away from the reaction cavity, of the mounting position in a lifting mode; the stirring assembly comprises a stirring driving part and a stirring part connected with the stirring driving part, the stirring part is located in the reaction cavity, the stirring driving part is connected with the lifting part and is driven by the lifting part to have a first position and a second position, and the stirring driving part is driven by the lifting part to rotate in the first position; at the first position, the stirring driving part is located at the first avoiding opening and matched with the stirring part, so that the stirring part stirs at the bottom of the reaction cavity, and at the second position, the stirring driving part is far away from the first avoiding opening, so that the reaction bag is convenient to replace. According to the bioreactor, when the disposable reaction bag is replaced, the stirring assembly does not need to be disassembled, time and labor are saved, the production efficiency is improved, the operation difficulty is small, and the production cost is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of biotechnological production equipment, and more particularly to a bioreactor. Background Art

[0002] As the core equipment for biotechnological production, disposable bioreactors are widely used in fields such as biopharmaceuticals, food processing, and modern agriculture. Especially in the production of high-end biological agents such as antibiotics, high-precision protein drugs, and cell vaccines, they have become indispensable equipment.

[0003] In the prior art, the magnetic stirrer in a disposable bioreactor is arranged at the bottom of the reactor. When replacing the disposable reaction bag, it is necessary to manually disassemble the magnetic stirrer to replace the disposable bioreaction bag on the magnetic stirrer. The whole process is time-consuming and laborious, and the operation difficulty is relatively large. Special skill training is required for the operators. Summary of the Invention

[0004] The present invention aims to at least solve one of the technical problems existing in the prior art. For this purpose, the present invention provides a bioreactor. When replacing the disposable reaction bag, the bioreactor does not require disassembling the stirring assembly, which saves time and effort, improves production efficiency, and has a relatively low operation difficulty, reducing production costs.

[0005] According to an embodiment of the present invention, the bioreactor includes a reaction tank having a reaction chamber and a mounting position provided at the bottom of the reaction tank. The mounting position is provided with a first avoidance opening. A lifting assembly, the lifting assembly includes a lifting member, and the lifting member is vertically movable and arranged on a side of the mounting position away from the reaction chamber. A stirring assembly, the stirring assembly includes a stirring driving member and a stirring member connected to the stirring driving member. The stirring member is located in the reaction chamber. The stirring driving member is connected to the lifting member and has a first position and a second position driven by the lifting member. In the first position, the stirring driving member is located at the first avoidance opening and cooperates with the stirring member to stir at the bottom of the reaction chamber. In the second position, the stirring driving member is away from the first avoidance opening to facilitate replacing the reaction bag.

[0006] According to some embodiments of the present invention, the stirring member is a rotary stirring member. In the first position, the rotary stirring member is spaced apart from the axis of the reaction tank, and the rotation axis of the rotary stirring member intersects the axis of the reaction tank. According to some embodiments of the present invention, the distance between the rotation center of the rotary stirring member and the axis of the reaction tank is d, and the distance between the axis of the reaction tank and the inner wall of the reaction tank is l, where (1 / 3)l ≤ d ≤ (1 / 2)l. According to some embodiments of the present invention, the angle between the rotation axis of the rotating stirring element and the axis of the reaction tank is α, and α=18°±5°. According to some embodiments of the present invention, the stirring assembly further comprises a magnetic member connected to the stirring driving member, and the stirring member and the magnetic member have opposite magnetism and are magnetically connected. According to some embodiments of the present invention, the stirring assembly also includes a magnetic shell and a stirring base, the magnetic shell has a accommodating cavity, the magnetic member is arranged in the accommodating cavity, the stirring member is rotatably connected to one side of the stirring base, and the other side of the stirring base is connected to the magnetic shell and is spaced apart from the magnetic member. According to some embodiments of the present invention, the stirring assembly further includes a stirring base, the stirring driving member is connected to the stirring base, and the stirring base is connected to the lifting assembly. According to some embodiments of the present invention, the stirring base includes a base mounting portion and a base connecting portion connected to the base mounting portion, the base mounting portion has a mounting cavity, the stirring drive member is installed in the mounting cavity, and the base connecting portion is connected to the lifting assembly. According to some embodiments of the present invention, the lifting assembly further comprises a support member, the support member is provided with a guide rail parallel to the axial direction of the reaction tank, and the stirring assembly is slidably connected to the guide rail. According to some embodiments of the present invention, the support member includes a first support and a second support connected to the first support at a certain angle, the second support is connected to a side of the mounting position away from the reaction chamber and is arranged along the axial direction of the reaction tank, and the guide rail is arranged on the second support. According to some embodiments of the present invention, the lifting member is a slider that slidably cooperates with the guide rail, and the slider is connected to the base connecting part. According to some embodiments of the present invention, the lifting assembly further includes a lifting drive member, which is connected to the first support, and an output shaft is connected to at least one of the base connection portion and the lifting member to drive the stirring assembly to lift and lower. In the present application, a bioreactor is provided, which is provided with a liftable lifting member, and the stirring drive member is connected to the lifting member, so that the stirring drive member can be lifted and lowered with the lifting member, and the stirring drive member has a second position away from the first avoidance port, so that the operator does not need to disassemble the stirring assembly, and can take out or place the disposable reaction bag at the open end of the reaction tank, thereby improving the replacement efficiency of the disposable reaction bag and the production efficiency. At the same time, the operating difficulty of the operator is reduced. Therefore, there is no need for special skill training for the staff, which reduces the production cost.

[0007] Additional aspects and advantages of the present invention will be given in part in the following description, become apparent in part from the following description, or be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1 is a schematic perspective view of a bioreactor according to an embodiment of the present application; Figure 2 is a top view of the bioreactor according to an embodiment of the present application; Figure 3 is a schematic view of the connection between a stirring assembly and a lifting assembly in the bioreactor according to an embodiment of the present application; Figure 4 is a partial exploded view of the stirring assembly in the bioreactor according to an embodiment of the present application; Figure 5 is a partial cross-sectional view of the stirring assembly in the bioreactor according to an embodiment of the present application.

[0009] Reference numerals: reactor 100, reaction tank 10, reaction chamber 10a, installation position 10b, first avoidance opening 11, second avoidance opening 12, lifting assembly 20, lifting member 21, support member 22, first support 221, second support 222, guide rail 222a, lifting driving member 23, stirring assembly 30, stirring member 31, magnetic member 32, magnetic housing 33, stirring chassis 34, stirring base 35, base installation portion 351, base connection portion 352, stirring driving member 36. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0010] Embodiments of the present invention will be described in detail below. Examples of the embodiments are shown in the drawings, where the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0011] The following disclosure provides many different embodiments or examples for implementing different structures of the present invention. To simplify the disclosure of the present invention, components and settings of specific examples are described below. Of course, they are merely examples and are not intended to limit the present invention. In addition, the present invention may repeat reference numerals and / or letters in different examples. Such repetition is for the purpose of simplification and clarity and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the present invention provides examples of various specific processes and materials, but those of ordinary skill in the art can realize the applicability of other processes and / or the use of other materials.

[0012] As the core equipment for biotechnological production, single-use bioreactors are widely used in fields such as biopharmaceuticals, food processing, and modern agriculture. Especially in the production of high-end biopreparations such as antibiotics, high-precision protein drugs, and cell vaccines, they have become indispensable equipment. The advantages of this type of equipment lie in its flexibility, ease of operation, and reduced risk of cross-contamination, making it of significant application value in laboratory research and development, pilot production, and small-scale commercial production.

[0013] In the prior art, the magnetic stirrer in a single-use bioreactor is installed at the bottom of the reactor. When it is necessary to replace the single-use reaction bag, the magnetic stirrer needs to be manually disassembled and then removed from the bottom of the reactor to replace the single-use reaction bag on the magnetic stirrer. Then, the magnetic stirrer needs to be manually reinstalled. The entire process is time-consuming and laborious, and the operation difficulty is relatively high. For example, there may be problems such as improper installation and inappropriate installation position during manual installation, which will affect the installation stability of the magnetic stirrer, as well as the stirring effect, and further affect cell growth, metabolite formation, and the overall process efficiency in the reactor. Therefore, special skill training for operators is required, which increases the production cost.

[0014] Based on this, the present invention proposes a bioreactor. By installing a lifting assembly at the installation position at the bottom of the reaction tank, the stirring driving member connected to the lifting assembly can be lifted and lowered on the side of the installation position away from the reaction chamber. When it is necessary to replace the single-use reaction bag, only need to lower the stirring driving member without disassembling the stirring structure. First, take out the filter element, and then replace the single-use reaction bag. When the single-use reaction bag is replaced, raise the stirring driving member to the first avoidance opening 11 without reinstallation, which improves the replacement efficiency of the single-use reaction bag, improves the production efficiency, and at the same time reduces the operation difficulty, does not require special skill training for staff, and reduces the production cost.

[0015] Figures 1-5 It is a schematic structural diagram of the bioreactor 100 according to an embodiment of the present invention. Refer to Figures 1-3 , the bioreactor 100 includes a reaction tank 10. The reaction tank 10 has a reaction chamber 10a, and the reaction chamber 10a is used to provide a reaction space for cell growth and metabolite formation. The reaction tank 10 may include a tank body and a cover body, and the cover body may be covered at the top opening of the tank body. The material of the reaction tank 10 may be glass, stainless steel, etc.

[0016] The bottom of the reaction tank 10 is provided with a mounting position 10b, which can be provided at a side of the bottom of the reaction tank 10 away from the reaction chamber 10a and recessed toward the side of the reaction chamber 10a to protect the structure provided at the mounting position 10b. The mounting position 10b is provided with a first avoidance opening 11, which is used to avoid the stirring drive member 36, so that the stirring drive member 36 can be magnetically connected to the stirring member 31.

[0017] The bioreactor 100 further includes a lifting assembly 20, which includes a lifting member 21. The lifting member 21 can be lifted and lowered and is arranged on the side of the installation position 10b away from the reaction chamber 10a. Specifically, the lifting route of the lifting member 21 can be a straight line, a curve, a broken line, etc. The lifting member 21 can be connected to the reaction tank 10, and can also be connected to other components of the lifting assembly 20, for example, a sliding connection, a rolling connection, a rotating connection, etc., to achieve lifting.

[0018] The bioreactor 100 further includes a stirring assembly 30 , which includes a stirring driving member 36 and a stirring member 31 connected to the stirring driving member 36 . The stirring member 31 is located in the reaction chamber 10 a , so that the stirring driving member 36 can be raised and lowered along with the lifting member 21 . The stirring drive member 36 has multiple positions under the lifting and driving of the lifting member 21. Specifically, the stirring drive member 36 can have a first position and a second position under the driving of the lifting member 21. In the first position, the stirring drive member is located at the first avoidance port 11. At this time, the stirring drive member 36 can cooperate with the stirring member 31 located in the reaction chamber 10a to stir at the bottom of the reaction chamber 10a, which is beneficial to improve the mixing effect. In the second position, the stirring drive member 36 is away from the first avoidance port 11, and the filter element can be taken out from the first avoidance port 11. At the same time, the operator can take out the disposable reaction bag from the reaction chamber 10a for replacement, and there is no need to disassemble the stirring assembly 30 from the bottom of the reaction tank 10 and install it to the bottom of the reaction tank 10, which improves the replacement efficiency of the disposable reaction bag and improves the production efficiency. At the same time, there is no need to consider the installation position and installation reliability issues that need to be considered when reinstalling the stirring assembly 30, thereby reducing the difficulty of operation. Therefore, there is no need for special skill training for the staff, reducing the production cost.

[0019] Therefore, refer to Figures 1-3, for the bioreactor 100 according to the present invention, by providing a liftable lifting member 21 and connecting the stirring drive member 36 to the lifting member 21, in this way, the stirring drive member 36 can be lifted and lowered with the lifting member 21, and the stirring drive member 36 has a second position away from the first avoidance opening 11. In this way, the operator can take out or place the disposable reaction bag at the opening end of the reaction tank 10 without disassembling and assembling the stirring assembly 30, improving the replacement efficiency of the disposable reaction bag, improving the production efficiency, and at the same time, reducing the operation difficulty of the operator. Therefore, there is no need for special skill training for the staff, reducing the production cost.

[0020] In some embodiments of the present invention, referring to Figure 2 and Figure 3 , the stirring member 31 is a rotary stirring member. In the first position, the rotary stirring member 31 is arranged at an interval from the axis of the reaction tank 10, and the rotation axis of the rotary stirring member 31 intersects the axis of the reaction tank 10.

[0021] Since the stirring assembly 30 is crucial for maintaining cell suspension, oxygen transfer and uniform distribution of nutrients in the bioreactor 100. In the prior art, the stirring member 31 of the bioreactor 100 is placed in the center of the bottom of the reaction tank 10. Such a setting has good symmetry, is easy to control, and is also simple in design and processing. However, in cell culture, cells are very sensitive to shear force and liquid mixing, and the mixing effect at the distal end of the impeller is poor.

[0022] Therefore, in the first position of the present application, the rotary stirring member 31 is arranged at an interval from the axis of the reaction tank 10, and the rotation axis of the rotary stirring member 31 intersects the axis of the reaction tank 10, that is, the rotary stirring member 31 is not arranged at the center of the bottom of the reaction tank 10 and forms a certain angle with the horizontal direction. In this way, the sediment at the bottom of the reactor 100 can be better treated, and the bottom material can be lifted by generating inclined streamlines, thereby avoiding solid settlement. At the same time, due to its asymmetric installation position, the rotary stirring member 31 can achieve a good mixing effect at a lower speed, thereby reducing the mechanical damage to cells.

[0023] In some embodiments of the present invention, referring to Figure 2 , the distance between the rotation center of the rotary stirring member 31 and the axis of the reaction tank 10 is d, and the distance between the axis of the reaction tank 10 and the inner wall of the reaction tank 10 is l, (1 / 3)l ≤ d ≤ (1 / 2)l.

[0024] It can be understood that the rotation center of the rotary stirring member 31 is a point of the rotation center of the rotary stirring, and the distance between it and the axis of the reaction tank 10 is the distance between the two when the rotation center is perpendicular to the axis of the reaction tank 10; the distance between the axis of the reaction tank 10 and the inner wall of the reaction tank 10 is the distance between two lines when the inner wall of the reaction tank 10 has a line parallel to the axis of the reaction tank 10. For example, when the reaction tank 10 is a hollow cylinder, l is the radius of the reaction tank 10.

[0025] By setting (1 / 3)l ≤ d ≤ (1 / 2)l, the rotary stirring member 31 has a good mixing effect and less mechanical damage to cells. Specifically, d can be set to (1 / 3)l, (2 / 5)l, (10 / 11)l, (1 / 2)l, etc.

[0026] In some embodiments of the present invention, referring to Figure 3 , the included angle between the rotation axis of the rotary stirring member 31 and the axis of the reaction tank 10 is α, and α = 18° ± 5°.

[0027] By setting α = 18° ± 5°, the mixing effect of the rotary stirring member 31 is further improved, and the mechanical damage to cells is reduced. Specifically, α can be set to 13°, 15°, 18°, 20°, 21°, 23°, etc. In some embodiments, the bottom surface of the reaction tank 10 can be set as an arc surface, and the curvature of the arc surface can be set such that when (1 / 3)l ≤ d ≤ (1 / 2)l, α = 18° ± 5°. In this way, when the stirring member 31 fits with the reaction tank 10, the setting requirements of the stirring member 31 are met. Of course, the present application is not limited to this. The bottom surface of the reaction tank 10 can also be set as a plane, and the stirring member 31 is connected to the lifting assembly 20 at a certain included angle to satisfy α = 18° ± 5°.

[0028] In some embodiments of the present invention, referring to Figure 4 and Figure 5 , the stirring assembly 30 further includes a magnetic member 32 connected to the stirring driving member 36. The stirring member 31 and the magnetic member 32 have opposite magnetisms and are magnetically connected.

[0029] By setting the magnetic member 32 and the stirring member 31 with opposite magnetisms, the stirring member 31 can rotate with the magnetic member 32 to achieve stirring.

[0030] It can be understood that the magnetic member 32 can be a magnet, etc. The stirring member 31 can be a propeller blade, etc.

[0031] In some embodiments, the stirring member 31 is used to connect with a disposable reaction bag and can be disposable. The stirring member 31 and the disposable reaction bag are placed into the reaction tank 10 through an opening. When the magnetic member 32 is located at the first avoidance opening 11, it attracts the stirring member 31, magnetically connects with the stirring member 31, and drives the stirring member 31 to rotate under the drive of the stirring drive member 36.

[0032] In some embodiments of the present invention, referring to Figure 4 and Figure 5 , the stirring assembly 30 further includes a magnetic housing 33 and a stirring chassis 34. The magnetic housing 33 has a receiving cavity, the magnetic member 32 is arranged in the receiving cavity, the stirring member 31 is rotatably connected to one side of the stirring chassis 34, and the other side of the stirring chassis 34 is connected to the magnetic housing 33 and is spaced apart from the magnetic member 32.

[0033] By providing the magnetic housing 33 and connecting the side of the stirring chassis 34 facing away from the stirring member 31 to the magnetic housing 33, the stirring chassis 34 is spaced apart from the magnetic member 32. In this way, when the magnetic member 32 rotates, it is not easy to generate frictional noise and chipping with the stirring chassis 34. At the same time, effective magnetic force transmission can be ensured to drive the stirring member 31 to rotate.

[0034] In some embodiments, the magnetic housing 33 includes an annular structure, the magnetic member 32 is arranged inside the annular structure, and along the axial direction of the magnetic member 32, the height of the magnetic member 32 is less than the height of the annular structure. In this way, the side of the stirring chassis 34 facing away from the stirring member 31 abuts against the magnetic housing 33, and thus can be spaced apart from the magnetic member 32. The stirring member 31 can be a rotating paddle.

[0035] It should be noted that the disposable reaction bag is installed on the stirring chassis 34, and the stirring member 31 is located inside the disposable reaction bag.

[0036] In some embodiments of the present invention, referring to Figure 3 , the stirring assembly 30 further includes a stirring base 35. The stirring drive member 36 is connected to the stirring base 35, and the stirring base 35 is connected to the lifting assembly 20.

[0037] By providing the stirring base 35, it is convenient to install the stirring drive member 36 and connect the rotating assembly 30 to the lifting assembly 20.

[0038] In some embodiments, the axis of the stirring base 35 can be parallel to the axis of the reaction tank 10; or it can form a certain angle with the axis of the reaction tank 10. The stirring drive member 36 can be a rotating motor or the like.

[0039] In some embodiments of the present invention, referring to Figure 3, the stirring base 35 includes a base mounting portion 351 and a base connecting portion 352 connected to the base mounting portion 351. The base mounting portion 351 has a mounting cavity, and the stirring driving member 36 is installed in the mounting cavity. The base connecting portion 352 is connected to the lifting assembly 20.

[0040] By providing the base mounting portion 351, it is convenient to install the stirring driving member 36. By providing the base connecting portion 352, it is convenient to connect the base mounting portion 351 with the lifting assembly 20.

[0041] Specifically, the base mounting portion 351 has a mounting cavity. The mounting cavity can be open at one end, facilitating the installation of the stirring driving member 36 from the open end into the mounting cavity. The other end of the mounting cavity can have a mounting opening, facilitating the output shaft of the stirring driving member 36 to extend out and connect to the magnetic member 32. Through holes can also be provided on the side of the mounting cavity to reduce the weight of the base mounting portion 351.

[0042] The base mounting portion 351 and the base connecting portion 352 can be fixedly connected, for example, by welding, integrally formed, etc.; they can also be detachably connected, for example, by threaded connection, snap connection, key connection, etc.; they can also be connected through a connecting member, etc.

[0043] The base connecting portion 352 can be plate-shaped, improving the reliability of connection with the lifting assembly 20. The base connecting portion 352 and the lifting assembly 20 can be fixedly connected, for example, by welding, integrally formed, etc.; they can also be detachably connected, for example, by threaded connection, snap connection, key connection, etc.; they can also be connected through a connecting member, etc.

[0044] In some embodiments of the present invention, referring to Figure 3 , the lifting assembly 20 further includes a support member 22. The support member 22 is provided with a guide rail 222a parallel to the axis direction of the reaction tank 10, and the stirring assembly 30 is slidably connected to the guide rail 222a.

[0045] By providing the guide rail 222a on the support member 22 and slidably connecting the stirring assembly 30 to the guide rail 222a, the stability of the lifting of the stirring assembly 30 is improved.

[0046] In some embodiments, the guide rail 222a can be one or more than two. When there are more than two guide rails 222a, the more than two guide rails 222a are spaced apart, and the base connecting portion 352 is slidably connected to the more than two guide rails 222a.

[0047] In some embodiments of the present invention, referring to Figure 3 , the support member 22 includes a first support 221 and a second support 222 connected to the first support 221 at a certain angle. The second support 222 is connected to the side of the mounting position 10b away from the reaction chamber 10a and is arranged along the axis direction of the reaction tank 10. The guide rail 222a is provided on the second support 222.

[0048] The support member 22 includes a second support 222. The second support 222 is connected to the side of the mounting position 10b facing away from the reaction chamber 10a, providing a supporting force for the support member 22. The first support 221 can be plate-shaped and has a hollow, so that it is convenient to connect the second support 222 and has a relatively small weight. The second support 222 is connected to the first support 221 and is used to set the guide rail 222a, so that the stirring assembly 30 can move up and down along the guide rail 222a. One or more second supports 222 can be provided. In the solution of this application, two second supports 222 are provided, and the two second supports 222 are arranged at intervals. The first support 221 and the second support 222 can be fixedly connected, for example, by welding, integral molding, etc.; they can also be detachably connected, for example, by screw connection, snap connection, key connection, etc.; they can also be connected through a connecting member, etc.

[0049] In some embodiments of the present invention, referring to Figure 3 , the lifting member 21 is a slider that slidably cooperates with the guide rail 222a, and the slider is connected to the base connection portion 352.

[0050] By providing the slider, it is convenient to slidably connect the base connection portion 352 with the slide rail.

[0051] In some embodiments, the guide rail 222a can be a sliding protrusion provided on the second support 222, and the slider can be provided with a sliding groove that cooperates with the sliding protrusion. Among them, the sliding protrusion can be trapezoidal, etc., and the size of the free end is larger than the size of the connection end connected to the second support 222. The sliding groove can be provided with a structure that cooperates with the sliding protrusion, so that the slider is not easily disengaged when slidingly connected to the guide rail 222a. Of course, it can be understood that the sliding protrusion can be provided on the slider and the sliding groove is provided on the second support 222.

[0052] In some embodiments, two second supports 222 are arranged at intervals, and the lifting member 21 is provided with two sliding grooves respectively corresponding to the sliding protrusions of the slide rails of the two second supports 222. The base connection portion 352 is connected to the slider between the two second supports 222, further improving the lifting stability of the stirring assembly 30.

[0053] In some embodiments of the present invention, referring to Figure 3 , the lifting assembly 20 further includes a lifting driving member 23. The lifting driving member 23 is connected to the first support 221, and the output shaft is connected to at least one of the base connection portion 352 and the lifting member 21 to drive the stirring assembly 30 to move up and down.

[0054] By providing the lifting driving member 23 to drive the lifting member 21 to slide along the slide rail.

[0055] The lifting drive member 23 can be a linear drive, for example, a linear motor, a cylinder, etc. The lifting drive member 23 is fixedly connected to the first support 221, and its output shaft can be connected to the base connection portion 352, or can be connected to the lifting member 21, or can also be connected to the base connection portion 352 and the lifting member 21 to drive the stirring assembly 30 to lift.

[0056] In some embodiments, a second avoidance opening 12 is further provided at the bottom of the reaction tank 10, and the output shaft of the lifting drive member can pass through the second avoidance opening 12.

[0057] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention.

[0058] In the present invention, unless otherwise clearly specified and defined, the terms "installed", "connected", "connected", "fixed", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection, an electrical connection, or a communication connection; it can be directly connected, or indirectly connected through an intermediate medium, and can be the internal communication of two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0059] In the present invention, unless otherwise clearly specified and defined, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or indicates that the first feature has a lower horizontal height than the second feature.

[0060] In the description of this specification, the descriptions referring to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples", etc., mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0061] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A bioreactor, characterized in that: include: A reaction tank, the reaction tank having a reaction chamber and a mounting position arranged at the bottom of the reaction tank, the mounting position being provided with a first avoidance opening; A lifting assembly, the lifting assembly comprising a lifting member, the lifting member being liftable and arranged at a side of the installation position away from the reaction chamber; A stirring component, wherein the stirring component includes a stirring drive member and a stirring member connected to the stirring drive member, wherein the stirring member is located in the reaction chamber, the stirring drive member is connected to the lifting member, and has a first position and a second position driven by the lifting member, wherein in the first position, the stirring drive member is located in the first avoidance port and cooperates with the stirring member so that the stirring member stirs at the bottom of the reaction chamber, and in the second position, the stirring drive member is away from the first avoidance port so as to replace the reaction bag.

2. The bioreactor according to claim 1, characterized in that The stirring member is a rotating stirring member. In the first position, the rotating stirring member is spaced apart from the axis of the reaction tank, and the rotation axis of the rotating stirring member intersects with the axis of the reaction tank.

3. The bioreactor according to claim 2, characterized in that The distance between the rotation center of the rotating stirring member and the axis of the reaction tank is d, the distance between the axis of the reaction tank and the inner wall of the reaction tank is l, (1 / 3)l≤d≤(1 / 2)l.

4. The bioreactor according to claim 2, characterized in that The angle between the rotation axis of the rotating stirring element and the axis of the reaction tank is α, α=18°±5°.

5. The bioreactor according to claim 1, characterized in that The stirring component also includes a magnetic member connected to the stirring driving member. The stirring member and the magnetic member have opposite magnetism and are magnetically connected.

6. The bioreactor according to claim 5, characterized in that The stirring assembly also includes a magnetic shell and a stirring base, the magnetic shell has a accommodating cavity, the magnetic piece is arranged in the accommodating cavity, the stirring piece is rotatably connected to one side of the stirring base, and the other side of the stirring base is connected to the magnetic shell and is spaced apart from the magnetic piece.

7. The bioreactor according to claim 2, characterized in that The stirring assembly also includes a stirring base, the stirring driving member is connected to the stirring base, and the stirring base is connected to the lifting assembly.

8. The bioreactor according to claim 7, characterized in that The stirring base comprises a base mounting portion and a base connecting portion connected to the base mounting portion, the base mounting portion has a mounting cavity, the stirring driving member is mounted in the mounting cavity, and the base connecting portion is connected to the lifting assembly.

9. The bioreactor according to claim 8, characterized in that The lifting assembly also includes a support member, the support member is provided with a guide rail parallel to the axis direction of the reaction tank, and the stirring assembly is slidably connected to the guide rail.

10. The bioreactor according to claim 9, characterized in that The support member includes a first support and a second support connected to the first support at a certain angle, the second support is connected to a side of the mounting position away from the reaction chamber and is arranged along the axial direction of the reaction tank, and the guide rail is arranged on the second support.

11. The bioreactor according to claim 9, characterized in that The lifting member is a slider that is slidably matched with the guide rail, and the slider is connected to the base connecting part.

12. The bioreactor according to claim 11, characterized in that The lifting assembly further includes a lifting driving member, which is connected to the first support, and an output shaft is connected to at least one of the base connection portion and the lifting member to drive the stirring assembly to move up and down.

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