Biopharmaceutical processing device and processing method

Abstract

The invention relates to the technical field of biopharmacy processing devices, in particular to a biopharmacy processing device and a processing method.The biopharmacy processing device comprises a processing box, a culture tank for biopharmacy is placed at the upper end of the processing box, an isolation plate is detachably installed at the inner end of the processing box, and a plurality of open holes are formed in the upper end of the isolation plate; open holes are formed in the culture tank, stable sleeves are fixedly mounted in the open holes, a plurality of sealing covers are arranged at the inner end of the culture tank in a penetrating manner, air outlet cylinders are slidably mounted at the inner ends of the sealing covers, movable pipes are slidably mounted at the inner ends of the stable sleeves, and cutoff devices for preventing liquid in the culture tank from flowing back are arranged in the movable pipes. By injecting bubbles, the oxygen concentration in a liquid phase can be increased, the transmission efficiency of oxygen can be improved, meanwhile, a stirring function is realized by utilizing the rising force of the bubbles, and gas stirring is a relatively mild stirring mode, so that the shearing force can be reduced, and cells can be protected.

Description

Technical Field

[0001] The present invention relates to the technical field of biopharmaceutical processing devices, and specifically to a device and processing method for biopharmaceutical processing. Background Art

[0002] Biopharmaceuticals refer to the process of producing therapeutic drugs using biotechnological means, including vaccines, antibodies, recombinant proteins, gene therapy drugs, etc. With the rapid development of the biopharmaceutical industry, the progress of production processes and equipment technologies has become a key factor in ensuring the quality and safety of drugs. In the biopharmaceutical processing process, the devices used not only need to meet strict aseptic requirements but also ensure the stability and controllability of production.

[0003] After retrieval, it is found that the prior art publication number is CN 112569841 A, which discloses an auxiliary device for biopharmaceutical processing, including a housing. The top of the housing is communicated with a thick cylinder, and a control mechanism is installed on the left side of the top of the housing. The control mechanism includes a base, a first motor, a curved plate, a curved groove, a circular plate, a straight rod, a first square block, and a second square block. The bottom of the base is fixedly connected to the left side of the top of the housing. This solution controls the speed of the medicine at the leakage port by controlling the left and right movement of the two square blocks on the inner wall of the thick cylinder, facilitating the mixing inside the device, reducing the rotational power source, reducing the load, realizing the mixed falling of the medicine through the cooperation of the two square blocks of the control mechanism and the thick cylinder, improving the mixing speed, improving the working efficiency, saving the trouble of later screening, saving time, shortening the working time, and facilitating the removal of the cylinder by opening the side of the housing through bolts and convex plates.

[0004] Therefore, based on the above retrieval and in combination with the existing technology, when the above solution uses a spiral blade to mix and stir biomass, the spiral blade will generate a relatively high shear force. For cells sensitive to shear force, the shear force generated by the rotation of the spiral blade during the cultivation process will damage the cells, resulting in cell rupture or death, thus affecting the production efficiency and the quality of the final product, and the practicability is not strong. For this reason, we propose a device and processing method for biopharmaceutical processing. Summary of the Invention

[0005] The purpose of the present invention is to provide a device and processing method for biopharmaceutical processing to solve the problems raised in the above background art.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: A device for biopharmaceutical processing, comprising a processing box, a culture tank for biopharmaceuticals is placed on the upper end of the processing box, an isolation plate is detachably installed on the inner end of the processing box, the isolation plate is located below the culture tank, a plurality of openings are opened on the upper end of the isolation plate, a stabilizing sleeve is fixedly installed in each of the openings, a plurality of sealing covers are passed through the inner end of the culture tank, and an air outlet is slidably installed on the inner end of the sealing cover, a plurality of bubble outlets for spraying oxygen are opened on the upper outer surface of the air outlet, when the culture tank is placed above the processing box, the air outlet is sleeved on the outer surface of the stabilizing sleeve, a movable tube is slidably installed on the inner end of the stabilizing sleeve, and a flow-breaking device for preventing the backflow of liquid in the culture tank is arranged inside the movable tube, a stabilizing tube is fixedly installed on the inner bottom end of the stabilizing sleeve, and the movable tube is sleeved on the outer surface of the stabilizing tube.

[0007] As a further solution of the present invention, an air pump for outputting oxygen is arranged on the right side of the processing box, the output end of the air pump is fixedly connected to a gas pipe, the output end of the gas pipe is fixedly connected to a plurality of branch pipes, and the output ends of the branch pipes are respectively passed through the interior of the stabilizing tube.

[0008] As a further solution of the present invention, a conical rubber sleeve is fixedly installed on the inner upper end of the stabilizing sleeve to prevent external contaminants from entering, a connecting ring is fixedly installed on the inner bottom end of the conical rubber sleeve, and a plurality of abutment rods are rotatably installed on the outer surface of the connecting ring. The abutment rods are distributed in a ring shape, and the outer surfaces of the abutment rods are in contact with the inner surface of the conical rubber sleeve, and the conical rubber sleeve is expanded when the abutment rods rotate.

[0009] As a further solution of the present invention, a movable ring is slidably installed on the inner end of the stabilizing sleeve, and the end of the abutment rod close to the conical rubber sleeve is fixedly connected to a traction rope, and the free end of the traction rope is fixedly connected to the movable ring. A plurality of rectangular through holes are provided on the outer surface of the stabilizing sleeve, and a plurality of force blocks are fixedly installed on the outer surface of the movable ring, and the force blocks are exposed on the outer surface of the stabilizing sleeve after passing through the rectangular through holes. When the culture trough is placed above the processing box, the bottom end of the punching bag contacts the upper end of the force block, so that it is more convenient to press the force block to move downward when the punching bag moves downward.

[0010] As a further solution of the present invention, a force ring is provided on the side where the abutment rods are close to each other, and an extension wire is fixedly connected to the end of the abutment rod close to the force ring. A wire hole is provided on the outer surface of the force ring, and the wire hole corresponds to the abutment rod. The free end of the extension wire is located inside the force ring after passing through the wire hole. The extension wire and the force ring are connected by a reset spring, and the reset spring is located inside the force ring. The reset spring is used to provide some resistance so that the conical rubber sleeve is not easy to open.

[0011] As a further solution of the present invention, the flow cutoff device includes a transfer airbag, the transfer airbag is fixedly installed at the inner end of the movable pipe, an air outlet pipe is fixedly installed at the upper end of the movable pipe, an output pipe is fixedly installed at the upper end of the transfer airbag, the output pipe corresponds to the air outlet pipe, and a sealing plate is fixedly installed at the inner end of the movable pipe.

[0012] As a further solution of the present invention, the sealing plate is located below the transfer airbag, a conduction pipe is fixedly connected to the bottom end of the sealing plate, one end of the conduction pipe away from the stable pipe is fixedly connected to the transfer airbag, the diameter of the conduction pipe is larger than the diameter of the output pipe, and when the gas ejected from the conduction pipe reaches the output pipe, due to the different volumes, part of the gas will stay in the transfer airbag.

[0013] As a further solution of the present invention, a stress ring is fixedly installed on the outer surface of the movable pipe, the upper end of the stress ring abuts against the bottom end of the movable ring, and air permeation holes are formed on the outer surface of the conduction pipe and are located inside the stable pipe.

[0014] As a further solution of the present invention, a central pipe is fixedly installed at the inner end of the conduction pipe, both the upper and lower ends of the central pipe are fixedly connected to the inner wall of the conduction pipe, a guiding through hole is formed on the outer surface of the conduction pipe, a sealing sleeve is slidably installed on the outer surface of the central pipe, a passive frame is slidably installed at the inner end of the movable pipe, the passive frame passes through the guiding through hole and is fixedly connected to the sealing sleeve, a rubber sheet is fixedly installed at one end of the passive frame close to the movable pipe, and the outer surface of the rubber sheet is in close contact with the inner wall of the movable pipe.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. When the present invention is used, during cell culture and fermentation, by injecting air bubbles, the oxygen concentration in the liquid phase can be increased, the oxygen transfer efficiency can be improved, and at the same time, the rising force of the air bubbles is used to achieve a stirring function. Gas stirring is a relatively gentle stirring method, which can reduce shear force and protect cells; 2. When the present invention is used, when the reaction ends, the transfer airbag will still eject gas, preventing the liquid in the culture tank from flowing back into the air outlet cylinder, thereby preventing waste, as well as preventing damage and blockage of the equipment, and at the same time ensuring that the transfer airbag slowly returns to normal pressure within a safe pressure range, avoiding unnecessary stress caused by too rapid pressure change. Description of the Drawings

[0016] Figure 1 It is a schematic structural diagram of a device for biopharmaceutical processing; Figure 2 It is a schematic structural diagram inside the processing box when the culture tank is taken out; Figure 3Exploded view of the culture tank; Figure 4 Schematic diagram of the internal structure of the stabilizing sleeve; Figure 5 Schematic diagram of the enlarged internal structure of the stabilizing sleeve; Figure 6 Schematic diagram of the positional relationship between the abutting rod and the stress ring; Figure 7 Schematic diagram of the internal structure of the movable tube; Figure 8 Schematic diagram of the enlarged internal structure of the movable tube; Figure 9 Schematic diagram of the structure at the sealing plate in the movable tube; Figure 10 Schematic diagram of the internal structure of the conduction tube; Figure 11 Working state diagram when the air outlet cylinder ejects bubbles.

[0017] In the figure: 1. Processing box; 2. Air delivery pipe; 3. Air pump; 4. Culture tank; 5. Partition board; 21. Shunt pipe; 22. Check valve; 101. Air outlet cylinder; 102. Sealing cover; 103. Stabilizing ring; 201. Conical rubber sleeve; 202. Stabilizing sleeve; 203. Movable ring; 204. Stabilizing tube; 205. Movable tube; 206. Stress block; 207. Tensile rope; 208. Abutting rod; 209. Connecting ring; 210. Stress ring; 211. Return spring; 212. Extension line; 213. Air outlet pipe; 301. Transfer airbag; 302. Return ring; 303. Conduction tube; 304. Stress ring; 305. Output pipe; 306. Vent hole; 307. Sealing plate; 401. Passive frame; 402. Abutting ring; 403. Central tube; 404. Guide through hole; 405. Rubber sheet; 406. Sealing sleeve. Detailed implementation method

[0018] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0019] Embodiment 1: Please refer to Figure 1 , 2, 3, 11, A device for biopharmaceutical processing, including a processing tank 1. A culture tank 4 for biopharmaceutical use is placed at the upper end of the processing tank 1. At one end of the processing tank 1 close to the culture tank 4, a plurality of rubber pads are fixedly installed. The upper end of the rubber pad contacts the upper edge of the culture tank 4 to reduce vibration and make the device more stable during operation. The inner end of the processing tank 1 is detachably installed with a partition plate 5 through bolts. The partition plate 5 is located below the culture tank 4. A number of openings are provided at the upper end of the partition plate 5, and stable sleeves 202 are fixedly installed in the openings. A plurality of sealing caps 102 penetrate through the inner end of the culture tank 4, and air outlet cylinders 101 are slidably installed at the inner ends of the sealing caps 102. A plurality of bubble outlets for ejecting oxygen are provided on the outer surface of the upper side of the air outlet cylinder 101. During cell culture and fermentation, during the process of injecting oxygen, the inside of the culture tank 4 can also be stirred, thereby reducing shear force and protecting cells. When the air outlet cylinder 101 is in the initial state, the bubble outlets are completely located inside the sealing cap 102. A sealing rubber ring is fixedly installed on the outer surface of the air outlet cylinder 101 and is closely attached to the inner wall of the sealing cap 102. The outer surface of the sealing cap 102 is threadedly connected with a stabilizing ring 103. The stabilizing ring 103 is located below the culture tank 4. Sealing rubber rings are fixedly installed at one end of the sealing cap 102 and the stabilizing ring 103 close to the culture tank 4 to improve airtightness; Please refer to Figure 2 , 3 , 4, When the culture tank 4 is placed above the processing tank 1, the air outlet cylinder 101 is sleeved on the outer surface of the stable sleeve 202. A movable tube 205 is slidably installed at the inner end of the stable sleeve 202, and a cut-off device for preventing the liquid inside the culture tank 4 from flowing back is provided inside the movable tube 205. A stable tube 204 is fixedly installed at the inner bottom end of the stable sleeve 202, and the movable tube 205 is sleeved on the outer surface of the stable tube 204. Specifically, a sealing rubber ring is fixedly installed at the inner end of the movable tube 205 to increase the airtightness between it and the stable tube 204; Such as Figure 1 , 2 As shown, an air pump 3 for outputting oxygen is provided on the right side of the processing tank 1. The air pump 3 is an existing mature technology and will not be elaborated here. The output end of the air pump 3 is fixedly connected with an air delivery pipe 2. The output end of the air delivery pipe 2 is fixedly connected with a plurality of shunt pipes 21, and the output ends of the shunt pipes 21 respectively penetrate through the inside of the stable tube 204.

[0020] Example 2: Please refer to Figure 4 , 56. A device for biopharmaceutical processing, based on Example 1, a conical rubber sleeve 201 is fixedly installed on the inner upper end of the stabilizing sleeve 202 to prevent external contaminants from entering, and the upper tip of the conical rubber sleeve 201 is in a closed state. When the culture tank 4 is taken out from the processing box 1, the conical rubber sleeve 201 is closed at the upper tip of the cone shape to isolate the stabilizing sleeve 202 from the outside. A connecting ring 209 is fixedly installed on the inner bottom end of the conical rubber sleeve 201, and a plurality of abutting rods 208 are rotatably installed on the outer surface of the connecting ring 209, and the abutting rods 208 are in a ring shape. The outer surface of the abutment rod 208 contacts the inner surface of the conical rubber sleeve 201, and the abutment rod 208 opens the conical rubber sleeve 201 when rotating, thereby realizing the opening of the top tip of the conical rubber sleeve 201. The inner end of the stabilizing sleeve 202 is slidably mounted with a movable ring 203. The end of the abutment rod 208 close to the conical rubber sleeve 201 is fixedly connected with a traction rope 207. The free end of the traction rope 207 is fixedly connected with the movable ring 203. When the movable ring 203 moves downward, the abutment rod 208 is dragged by the traction rope 207 to rotate toward the conical rubber sleeve 201. The outer surface of the stabilizing sleeve 202 is provided with a plurality of rectangular through holes, and the outer surface of the movable ring 203 is fixedly provided with a plurality of force blocks 206, and the force blocks 206 are exposed on the outer surface of the stabilizing sleeve 202 after passing through the rectangular through holes. When the culture tank 4 is placed above the processing box 1, the bottom end of the air outlet 101 contacts the upper end of the force block 206, and the interior of the culture tank 4 is filled with nutrient water and other materials for reaction, so that the culture tank 4 becomes heavier as a whole, and then the pressure squeezes the upper part of the air outlet 101. At this time, the air outlet 101 is under the influence of the pressure. The force ring 210 is pressed downward by the force block 206. Specifically, a force ring 210 is provided on the side where the abutting rods 208 are close to each other. An extension wire 212 is fixedly connected to one end of the abutting rod 208 close to the force ring 210. A wire hole is provided on the outer surface of the force ring 210. The wire hole corresponds to the abutting rod 208. The free end of the extension wire 212 is located inside the force ring 210 after passing through the wire hole. The extension wire 212 and the force ring 210 are connected by a return spring 211. The return spring 211 is located inside the force ring 210. See also Figure 7 , 89. The interruption device includes a transfer airbag 301. The transfer airbag 301 is fixedly installed with the inner end of the movable tube 205. The upper end of the movable tube 205 is fixedly welded with an air outlet pipe 213. The upper end of the air outlet pipe 213 is provided with an air outlet, and the air outlet corresponds to the conical rubber sleeve 201. Specifically, a reset ring 302 is fixedly installed on the outer surface of the transfer airbag 301. The reset ring 302 is elastic. When the transfer airbag 301 expands, the reset ring 302 is propped up. An output pipe 305 is fixedly installed on the upper end of the transfer airbag 301. The output pipe 305 corresponds to the air outlet pipe 213. A sealing plate 307 is fixedly installed on the inner end of the movable tube 205. The sealing plate 307 is located below the transfer airbag 301. The bottom end of the sealing plate 307 is fixedly connected with a conducting tube 303. One end of the conducting tube 303 away from the stabilizing tube 204 is fixedly connected to the transfer airbag 301. The diameter of the conducting tube 303 is larger than the diameter of the output tube 305. When the conducting tube 303 blows out gas, the gas passes through the transfer airbag 301 and comes to the output tube 305. Since the diameter of the conducting tube 303 is larger than the diameter of the output tube 305, the output gas volume is larger than the volume received by the output tube 305. At this time, the excess gas accumulates in the transfer airbag 301 and props up the transfer airbag 301 to make it in an expanded state. A one-way valve 22 is fixedly installed on the upper end of the stabilizing tube 204, the output end of the shunt tube 21 is fixedly connected to the one-way valve 22, and a force ring 304 is fixedly welded on the outer surface of the movable tube 205, and the upper end of the force ring 304 abuts against the bottom end of the movable ring 203; An air hole 306 is provided on the outer surface of the conducting tube 303, and the air hole 306 is located inside the stabilizing tube 204. When the gas blown out from the diverter tube 21 is blown out from the one-way valve 22 and enters between the sealing plate 307 and the stabilizing tube 204, the gas cannot flow and pushes the movable tube 205 to move upward, and the conducting tube 303 also moves upward until the air hole 306 moves above the stabilizing sleeve 202, and the gas enters the conducting tube 303 from the air hole 306.

[0021] Example 3: Please refer to Figure 8 , 910. A device for biopharmaceutical processing, based on Example 2, a central tube 403 is fixedly installed on the inner end of the guide tube 303, the central tube 403 is wide at the upper and lower ends and narrow at the center, the upper and lower ends of the central tube 403 are fixedly connected to the inner wall of the guide tube 303, a guide hole 404 is opened on the outer surface of the guide tube 303, a sealing sleeve 406 is slidably installed on the outer surface of the central tube 403, a passive frame 401 is slidably installed on the inner end of the movable tube 205, the passive frame 401 is fixedly connected to the sealing sleeve 406 after passing through the guide hole 404, the length of the guide hole 404 is less than the distance moved by the guide tube 303, the bottom end of the sealing plate 307 is fixedly installed with an abutment ring 402, and the end of the passive frame 401 close to the movable tube 205 is fixedly installed with a rubber sheet 405, the outer surface of the rubber sheet 405 The surface is in close contact with the inner wall of the movable tube 205. When the movable tube 205 moves up and down, the passive frame 401 is driven to move by the friction between the rubber sheet 405 and the inner wall of the movable tube 205. A through hole is opened on the outer surface of the central tube 403. A limit block is fixedly installed on the outer surface of the central tube 403. The limit block is located above the through hole. The sealing sleeve 406 is sleeved on the outer surface of the through hole. When the movable tube 205 moves upward at this time, the passive frame 401 drives the sealing sleeve 406 to move upward under the friction between the rubber sheet 405 and the inner wall of the movable tube 205, and completely covers the through hole under the action of the limit block. On the contrary, the sealing sleeve 406 moves downward and exposes the through hole. At the same time, the diameter of the conducting hole 404 is larger than the diameter of the passive frame 401, so that the conducting hole 404 can realize the circulation of gas.

[0022] The working principle of the present invention is: When in use, the culture tank 4 filled with liquid is placed above the processing box 1. At this time, the bottom end of the air outlet 101 contacts the upper end of the force block 206, and the force block 206 is pressed downward. When the force block 206 moves downward, it drives the movable ring 203 to move downward, and the abutment rod 208 is pulled by the traction rope 207 to rotate in the direction of the conical rubber sleeve 201, and the sharp mouth above the conical rubber sleeve 201 is opened. At this time, the air pump 3 outputs gas and passes through the air supply pipe 2 and the shunt pipe 21, and then it is ejected from the one-way valve 22, and the gas enters When the gas reaches between the sealing plate 307 and the stabilizing tube 204, the gas cannot circulate and pushes the movable tube 205 upward, and the conducting tube 303 also moves upward until the vent hole 306 moves above the stabilizing tube 204. Then, the gas enters the conducting tube 303 from the vent hole 306, and then the gas enters the transfer airbag 301 from the central tube 403. Since the diameter of the conducting tube 303 is larger than the diameter of the output tube 305, the excess air props up the transfer airbag 301, and the reset ring 302 is in a stretched state at this time. Meanwhile, during the upward movement of the movable tube 205, the force-receiving ring 304 abuts against the movable ring 203. Under the interaction of forces, the force-receiving block 206 lifts the air outlet cylinder 101, and the bubble outlet on the outer surface of the air outlet cylinder 101 is exposed in the liquid in the culture tank 4. Subsequently, the gas blown out from the output pipe 305 reaches the air outlet cylinder 101 through the air outlet pipe 213. Immediately afterwards, the gas flows out from the bubble outlet on the outer surface of the air outlet cylinder 101 and stirs the inside of the culture tank 4. At the same time, it can also provide oxygen for the internal microorganisms. Since the pressure of the blown gas is greater than the pressure of the liquid in the culture tank 4, there is no need to worry about the occurrence of liquid penetration; Then when the processing stops, the air pump 3 stops working at this time. Under the action of the elastic force of the reset ring 302, the residual air inside the transfer air bag 301 is squeezed. There is still gas escaping from the bubble outlet on the outer surface of the air outlet cylinder 101, thus avoiding the reverse infiltration of the liquid inside the culture tank 4 into the air outlet cylinder 101. At the same time, under the action of the pressure of the liquid inside the culture tank 4 and gravity, the air outlet cylinder 101 is pressed downward. The force-receiving block 206 is pushed to move the force-receiving ring 304 downward, and the movable tube 205 is moved downward. Then during the downward movement of the movable tube 205, under the action of the friction force between the inner wall of the movable tube 205 and the rubber sheet 405, the passive frame 401 is moved downward, and the through hole on the outer surface of the central tube 403 is exposed. At this time, the air between the stable tube 204 and the sealing plate 307 continues to flow into the transfer air bag 301. The gas pressure at this time is not as strong as the downward pressing force of the air outlet cylinder 101. When the air outlet cylinder 101 completely falls, the culture tank 4 can be taken out of the processing box 1.

[0023] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered within the protection scope of the present invention.

Claims

1. A device for biopharmaceutical processing, comprising a processing box (1), characterized in that: A culture tank (4) for biopharmaceuticals is placed at the upper end of the processing box (1), and an isolation plate (5) is detachably mounted on the inner end of the processing box (1). The isolation plate (5) is located below the culture tank (4), and a plurality of openings are formed on the upper end of the isolation plate (5). Stable sleeves (202) are fixedly mounted in the openings. A plurality of sealing covers (102) are passed through the inner end of the culture tank (4), and a gas outlet (101) is slidably mounted on the inner end of each sealing cover (102). The upper outer surface of the gas outlet (101) is opened. A plurality of bubble outlets for ejecting oxygen are provided. When the culture tank (4) is placed above the processing box (1), the gas outlet tube (101) is sleeved on the outer surface of the stabilizing sleeve (202). A movable tube (205) is slidably mounted on the inner end of the stabilizing sleeve (202), and a flow-blocking device for preventing the liquid inside the culture tank (4) from flowing back is arranged inside the movable tube (205). A stabilizing tube (204) is fixedly mounted on the inner bottom end of the stabilizing sleeve (202), and the movable tube (205) is sleeved on the outer surface of the stabilizing tube (204).

2. The device for biopharmaceutical processing according to claim 1, characterized in that: An air pump (3) for outputting oxygen is arranged on the right side of the processing box (1); the output end of the air pump (3) is fixedly connected to an air supply pipe (2); the output end of the air supply pipe (2) is fixedly connected to a plurality of branch pipes (21); and the output ends of the branch pipes (21) are respectively arranged inside the stabilizing pipe (204).

3. The device for biopharmaceutical processing according to claim 1, characterized in that: A conical rubber sleeve (201) is fixedly mounted on the inner upper end of the stabilizing sleeve (202) to prevent external contaminants from entering, a connecting ring (209) is fixedly mounted on the inner lower end of the conical rubber sleeve (201), and a plurality of abutment rods (208) are rotatably mounted on the outer surface of the connecting ring (209), the abutment rods (208) are distributed in a ring shape, the outer surfaces of the abutment rods (208) are in contact with the inner surface of the conical rubber sleeve (201), and the abutment rods (208) open the conical rubber sleeve (201) when rotating.

4. The device for biopharmaceutical processing according to claim 3, characterized in that: A movable ring (203) is slidably mounted on the inner end of the stabilizing sleeve (202); one end of the abutting rod (208) close to the conical rubber sleeve (201) is fixedly connected to a traction rope (207); the free end of the traction rope (207) is fixedly connected to the movable ring (203); a plurality of rectangular through holes are provided on the outer surface of the stabilizing sleeve (202); a plurality of force blocks (206) are fixedly mounted on the outer surface of the movable ring (203); and the force blocks (206) are exposed on the outer surface of the stabilizing sleeve (202) after passing through the rectangular through holes; when the culture tank (4) is placed above the processing box (1), the bottom end of the air outlet bag (101) contacts the upper end of the force block (206).

5. The device for biopharmaceutical processing according to claim 4, characterized in that: A stress ring (210) is provided on one side of the abutment rods (208) close to each other, and an extension wire (212) is fixedly connected to one end of the abutment rod (208) close to the stress ring (210). A wire hole is provided on the outer surface of the stress ring (210), and the wire hole corresponds to the abutment rod (208). The free end of the extension wire (212) is located inside the stress ring (210) after passing through the wire hole. The extension wire (212) and the stress ring (210) are connected via a return spring (211), and the return spring (211) is located inside the stress ring (210).

6. The device for biopharmaceutical processing according to claim 1, characterized in that: The flow interrupting device comprises a transfer airbag (301), the transfer airbag (301) being fixedly mounted on the inner end of a movable tube (205), an air outlet pipe (213) being fixedly mounted on the upper end of the movable tube (205), an output pipe (305) being fixedly mounted on the upper end of the transfer airbag (301), the output pipe (305) corresponding to the air outlet pipe (213), and a sealing plate (307) being fixedly mounted on the inner end of the movable tube (205).

7. The device for biopharmaceutical processing according to claim 6, characterized in that: The sealing plate (307) is located below the transfer airbag (301), and a conducting tube (303) is fixedly connected to the bottom end of the sealing plate (307), and one end of the conducting tube (303) away from the stabilizing tube (204) is fixedly connected to the transfer airbag (301), and the diameter of the conducting tube (303) is greater than the diameter of the output tube (305).

8. The device for biopharmaceutical processing according to claim 7, characterized in that: A force-bearing ring (304) is fixedly mounted on the outer surface of the movable tube (205), the upper end of the force-bearing ring (304) abuts against the bottom end of the movable ring (203), and an air hole (306) is provided on the outer surface of the conducting tube (303), and the air hole (306) is located inside the stabilizing tube (204).

9. The device for biopharmaceutical processing according to claim 8, characterized in that: A central tube (403) is fixedly mounted on the inner end of the conducting tube (303); the upper and lower ends of the central tube (403) are fixedly connected to the inner wall of the conducting tube (303); a conducting hole (404) is provided on the outer surface of the conducting tube (303); a sealing sleeve (406) is slidably mounted on the outer surface of the central tube (403); a passive frame (401) is slidably mounted on the inner end of the movable tube (205); the passive frame (401) is fixedly connected to the sealing sleeve (406) after passing through the conducting hole (404); a rubber sheet (405) is fixedly mounted on one end of the passive frame (401) close to the movable tube (205); the outer surface of the rubber sheet (405) is in close contact with the inner wall of the movable tube (205).

10. A method for processing a biopharmaceutical processing device, applied to a biopharmaceutical processing device according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: Place the culture tank (4) filled with liquid above the processing box (1). At this time, the bottom end of the air outlet tube (101) contacts the upper end of the force block (206), and the force block (206) is pressed downward. When the force block (206) moves downward, it drives the movable ring (203) to move downward, and pulls the abutment rod (208) toward the direction of the conical rubber sleeve (201) through the traction rope (207), and opens the tip above the conical rubber sleeve (201). At this time, the air pump (3) outputs gas and passes through the air supply pipe (2) and the shunt pipe (21), and then it is ejected from the one-way valve (22), and the gas enters When the gas is between the sealing plate (307) and the stabilizing tube (204), the gas cannot circulate and pushes the movable tube (205) upward, and the conducting tube (303) also moves upward until the air vent (306) moves above the stabilizing sleeve (202). Then, the gas enters the conducting tube (303) from the air vent (306), and then the gas enters the transfer airbag (301) from the central tube (403). Since the diameter of the conducting tube (303) is larger than the diameter of the output tube (305), the excess air props up the transfer airbag (301), and the reset ring (302) is in a stretched state at this time. S2: The force-bearing circle (304) is pressed against the movable ring (203), and under the interaction of forces, the force-bearing block (206) lifts the gas outlet cylinder (101), and the bubble outlet on the outer surface of the gas outlet cylinder (101) is exposed in the liquid in the culture tank (4). Then, the gas blown out from the output pipe (305) enters the gas outlet cylinder (101) through the gas outlet pipe (213), and then the gas flows out from the bubble outlet on the outer surface of the gas outlet cylinder (101), stirring the culture tank (4) and providing oxygen to the internal microorganisms; S3: When the air pump (3) stops working, the elastic force of the reset ring (302) squeezes the residual air inside the transfer airbag (301), and gas still comes out of the bubble outlet on the outer surface of the air outlet tube (101), thereby preventing the liquid inside the culture tank (4) from back-seeping into the air outlet tube (101). At the same time, under the pressure of the liquid inside the culture tank (4) and gravity, the air outlet tube (101) is pressed downward, and the force-bearing circle (304) is pushed downward by the force-bearing block (206), and the movable tube (205) is moved downward. Then, in the process of the movable tube (205) moving downward, the friction between the inner wall of the movable tube (205) and the rubber sheet (405) causes the passive frame (401) to move downward, and the through hole on the outer surface of the central tube (403) is exposed. At this time, the air between the stabilizing tube (204) and the sealing plate (307) continues to flow into the transfer airbag (301).

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