Counterweight-adjustable intelligent confocal culture dish

By designing an intelligent confocal petri dish with adjustable counterweights, combining operating components and matching components, the problems of environmental changes and inlet and outlet gas after sampling of the petri dish are solved, and the stability of culture and observation accuracy are improved.

CN120059910AActive Publication Date: 2025-05-30SUZHOU UNIV
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Patent Information

Application Number
CN202510280418.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-11
Publication Date
2025-05-30
Estimated Expiration
2045-03-11

AI Technical Summary

Technical Problem

During the use of the Petri dish, environmental changes after sampling lead to abnormal culture, and the inlet and outlet gas is not isolated, affecting the stability of the culture environment.

Method used

An intelligent confocal culture dish with adjustable counterweights is designed. By combining the operating components and the mating components, the stable fixation of the fixed support plate and the flatness adjustment of the liquid level are achieved. The filtering and purification barrel and exhaust fixing pipe are used to filter and isolate the inlet and outlet gas.

Benefits of technology

It effectively avoids culture abnormalities caused by environmental changes, improves the stability of culture and the accuracy of observation, and reduces the impact of the external environment on the culture environment through isolation treatment.

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Abstract

The invention discloses an intelligent confocal culture dish with an adjustable balance weight, and relates to the technical field of biological culture equipment. A limiting support ring is mounted on the inner side of a treatment fixing barrel, a plurality of clamping limiting columns are welded to the top end of the limiting support ring at equal intervals, and a fixing support disc sleeves the top end of the limiting support ring; a plurality of insertion connecting openings are formed in the top end of the fixed supporting disc at equal intervals, a clamping threaded groove is formed in the outer side end of the treatment fixed barrel, a clamping threaded ring is connected to the top of the outer side of the treatment fixed barrel through threads, an operation combined ring is rotationally connected to the inner side of the clamping threaded ring, and limiting supporting boxes are welded to the bottom end of the operation combined ring at equal intervals; the culture dish effectively solves the problems that in the prior art, due to the fact that a culture dish is of an integral type and cannot be separated for use, the internal environment of the culture dish is greatly changed during use and observation, and the culture stability is affected, meanwhile, the inside and the outside are isolated, the influence of the external environment on the culture environment is reduced, and the culture stability is improved.
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Description

Technical Field

[0001] The present invention relates to the technical field of biological culture equipment, and specifically relates to an intelligent confocal petri dish with adjustable counterweight. Background Technique

[0002] A petri dish is a laboratory utensil used for culturing microorganisms or cells. It consists of a flat disc-shaped bottom and a lid, usually made of glass or plastic, and there are disposable and reusable ones. It is suitable for operations such as inoculation, streaking, and separating bacteria in the laboratory. In confocal microscopy, in the imaging system, a point light source is used to illuminate the sample, and the light carrying the sample information is collected by a point detector, and finally the three-dimensional information of the entire sample is obtained by using transverse and axial scanning techniques.

[0003] However, during the use of the petri dish, since the biological liquid in the petri dish needs to be placed in a fixed culture environment for cultivation, when observing, it is necessary to take samples from the culture environment and place them in the confocal petri dish for observation. During the sampling operation, due to the environmental change and the need to use equipment for handling, the sampled organisms are prone to death or overall damage under the influence of handling and the environment, which greatly affects the accuracy of observation. Summary of the Invention

[0004] The present invention provides an intelligent confocal petri dish with adjustable counterweight, which can effectively solve the problems proposed in the above background technique that during the use of the petri dish, the environment changes after the petri dish is taken out, which greatly affects the subsequent normal cultivation, and the air intake and exhaust ventilation are not isolated, resulting in the interconnection of the environments and further affecting the culture environment.

[0005] To achieve the above object, the present invention provides the following technical solution: An intelligent confocal petri dish with adjustable counterweight, including a processing fixed barrel, and the processing fixed barrel is provided with an operation component;

[0006] The operation component includes a limiting support ring;

[0007] The limiting support ring is installed inside the processing fixed barrel. A number of clamping and limiting columns are welded equidistantly at the top of the limiting support ring. A fixed support disc is sleeved at the top of the limiting support ring, and a number of insertion connection ports are opened equidistantly at the top of the fixed support disc;

[0008] A clamping thread groove is opened at the outer end of the processing fixed barrel. A clamping thread ring is connected to the outer top of the processing fixed barrel by thread. An operation combination ring is rotatably connected to the inner side of the clamping thread ring. A number of limiting support boxes are welded equidistantly at the bottom end of the operation combination ring. A number of pressing springs are welded equidistantly at the top inner side of the limiting support box. The bottom ends of the plurality of pressing springs are welded to a pressing processing box, and an anti-slip processing pad is adhered to the bottom end of the pressing processing box;

[0009] A number of inner hole damping blocks are welded at equal intervals on the outer side end of the engaging thread ring. A combined processing column is sleeved inside the inner hole damping block, and a limiting fitting frame is welded at the top ends of multiple said combined processing columns.

[0010] According to the above technical solution, the engaging limiting column is sleeved inside the insertion connection port, and the longitudinal section of the fixed support disk is T-shaped.

[0011] According to the above technical solution, the top of the side end of the fixed support disk is attached to the inner side end of the processing fixed barrel, and the bottom end of the operation combined ring is attached to the top end of the processing fixed barrel.

[0012] According to the above technical solution, the pressing processing box and the limiting support box are slidably sleeved, and the bottom end of the anti-slip processing pad is attached to the top end of the fixed support disk.

[0013] According to the above technical solution, the cross section of the anti-slip processing pad is arc-shaped, and the bottom end of the limiting fitting frame is engaged and connected to the top end of the engaging thread ring.

[0014] According to the above technical solution, a clamping limit cover is rotatably connected inside the limiting fitting frame, and a sealing fixing ring is adhered to the outer bottom end of the clamping limit cover;

[0015] A number of exhaust fixing pipes are equidistantly penetrated and connected to the top end of the clamping limit cover. One end of multiple said exhaust fixing pipes is penetrated and connected to a filtering and purifying barrel, and an inlet and outlet operation pipe is penetrated and connected to the top end of the filtering and purifying barrel.

[0016] According to the above technical solution, the filtering and purifying barrel is clamped to the top end of the clamping limit cover, and the side end of the sealing fixing ring is respectively attached to the side end of the limiting fitting frame and the side end of the operation combined ring.

[0017] According to the above technical solution, the processing fixed barrel is provided with a matching component;

[0018] The matching component includes a limiting magnetic ring;

[0019] A limiting magnetic ring is sleeved on the outer side end of the processing fixed barrel. A connecting fitting frame is rotatably connected to the top end of the limiting magnetic ring. A number of counterweight blocks are equidistantly sleeved inside the connecting fitting frame, and a number of supporting and buffering blocks are equidistantly adhered to the inner side end of the connecting fitting frame;

[0020] A permanent magnetic ring is fixed in the middle of the inner side of the clamping limit cover. A connecting magnetic ring is magnetically attracted to the bottom end of the permanent magnetic ring. Isolation net plates are fixed to both the top and bottom of the inner side of the connecting magnetic ring. An irregular air inlet hole is opened at the top end of the inner side of the clamping limit cover, and a porous sealing ring is rotatably connected to the top of the inner side of the irregular air inlet hole.

[0021] According to the above technical solution, the outer diameter of the connecting magnetic ring is equal to the inner diameter of the clamping limit cover, and there are two isolation net plates.

[0022] According to the above technical solution, the longitudinal section of the counterweight is T-shaped, and two adjacent counterweights are sleeved with each other.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] 1. An operation component is provided. The fixed support disk is fixed to the top of the limiting support ring through the clamping limiting column and the insertion connection port to realize the taking and placing process of the fixed support disk. The clamping threaded groove is used to connect and combine the clamping threaded ring and the processing fixed barrel, and the operation combination ring is fixed to the top of the processing fixed barrel. The pressing spring drives the downward pressing processing box and the anti-slip processing pad to move down to the top of the fixed support disk to press the fixed support disk, ensuring the stability of the placement of the fixed support disk, so that the culture dish can be taken and placed according to needs during culture and observation, avoiding the occurrence of abnormal culture caused by the change of the culture environment and the inability to recover in a short time. The limiting cooperation frame and the clamping limiting cover are fixed to the top of the processing fixed barrel through the combined processing column and the inner hole damping block. The air inlet and outlet are carried out bidirectionally through the inlet and outlet operation pipe and the exhaust fixing pipe, and the filter purification barrel is used to filter the inlet and exhaust gases, reducing the situation that the environment is affected due to gas colluding and not being isolated, effectively solving the problem that in the prior art, because the culture dish is integral and cannot be separated and taken, the internal environment of the culture dish changes greatly during taking and observation, affecting the stability of culture. At the same time, the internal and external are isolated to reduce the influence of the external environment on the culture environment and improve the stability of culture.

[0025] 2. A cooperation component is provided. The isolation net plate is sleeved inside the clamping limiting cover by magnetically attracting and fixing the connecting magnetic ring with the permanent magnet ring to realize the connection of the net plate, so that the equipment can be quickly replaced during isolation and protection. By rotating the connecting cooperation frame, the position of the counterweight is switched, and the connecting cooperation frame is magnetically attracted and clamped by the limiting magnetic ring. The counterweight is placed inside the connecting cooperation frame according to needs to realize counterweight processing and counterweight adjustment, so that the liquid level can be adjusted according to the flatness during microscope observation and culture, ensuring the stability of observation and culture during processing. By rotating the porous sealing ring, the air inlet and outlet size of the special-shaped air inlet hole is adjusted to realize the adjustment of air inlet and outlet and ensure the stability of air inlet and outlet. The liquid level of the culture dish is adjusted by using multiple groups of adjustment cooperation linkages to improve the stability of culture and the accuracy of observation.

[0026] In summary, through the mutual cooperation of the operation component and the cooperation component, and through the multi-position separation processing of the culture dish, the abnormal culture caused by excessive environmental changes can be reduced during the sampling observation and continuous culture processes. At the same time, through the counterweight adjustment and the isolation air inlet adjustment, the liquid level control and the culture environment control are realized, thereby effectively improving the stability of culture and the accuracy of observation. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation to the present invention. In the accompanying drawings:

[0028] Figure 1 is a schematic three-dimensional structure diagram of the present invention;

[0029] Figure 2 is a schematic structure diagram of the operation component of the present invention;

[0030] Figure 3 is a schematic installation structure diagram of the engaging limiting post of the present invention;

[0031] Figure 4 is a schematic installation structure diagram of the limiting support box of the present invention;

[0032] Figure 5 is a schematic installation structure diagram of the engaging limiting cover of the present invention;

[0033] Figure 6 is a schematic structure diagram of the cooperating component of the present invention;

[0034] Figure 7 is a schematic installation structure diagram of the special-shaped air inlet hole of the present invention;

[0035] Figure 8 is a schematic installation structure diagram of the counterweight of the present invention;

[0036] Reference numerals in the figure: 1, processing fixed barrel;

[0037] 2, operation component; 201, limiting support ring; 202, engaging limiting post; 203, fixed support disk; 204, insertion connection port; 205, engaging thread groove; 206, engaging thread ring; 207, operation combination ring; 208, limiting support box; 209, pressing spring; 210, downward pressing processing box; 211, anti-slip processing pad; 212, inner hole damping block; 213, combination processing column; 214, limiting cooperation frame; 215, engaging limiting cover; 216, sealing fixed ring; 217, exhaust fixed pipe; 218, filtering and purifying barrel; 219, in-out operation pipe;

[0038] 3, cooperating component; 301, limiting magnetic ring; 302, connection cooperation frame; 303, counterweight; 304, support buffer block; 305, permanent magnetic ring; 306, connection magnetic ring; 307, isolation net plate; 308, special-shaped air inlet hole; 309, porous sealing ring. Detailed implementation manners

[0039] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only for explaining and illustrating the present invention and are not used to limit the present invention.

[0040] Embodiment: As Figure 1-8 shown, the present invention provides a technical solution, an intelligent confocal culture dish with adjustable counterweight, including a processing and fixing barrel 1, and the processing and fixing barrel 1 is provided with an operating component 2;

[0041] The operating component 2 includes a limiting support ring 201, a clamping limiting column 202, a fixing support disk 203, an insertion connection port 204, a clamping thread groove 205, a clamping thread ring 206, an operating combination ring 207, a limiting support box 208, a pressing spring 209, a downward pressing processing box 210, an anti-slip processing pad 211, an inner hole damping block 212, a combination processing column 213, a limiting cooperation frame 214, a clamping limiting cover 215, a sealing fixing ring 216, an exhaust fixing pipe 217, a filtering and purifying barrel 218, and an access operating pipe 219;

[0042] The limiting support ring 201 is installed inside the processing and fixing barrel 1. A plurality of clamping limiting columns 202 are welded equidistantly at the top of the limiting support ring 201. The clamping limiting columns 202 are sleeved inside the insertion connection port 204 to achieve stable connection and support, ensuring the stability of the overall alignment and limitation. The fixing support disk 203 is sleeved at the top of the limiting support ring 201. The longitudinal section of the fixing support disk 203 is T-shaped. The top side of the fixing support disk 203 is attached to the inner side end of the processing and fixing barrel 1 to achieve sleeve connection and ensure the stability of the alignment and limitation. A plurality of insertion connection ports 204 are opened equidistantly at the top of the fixing support disk 203;

[0043] The clamping thread groove 205 is opened at the outer side end of the processing and fixing barrel 1. The clamping thread ring 206 is threadedly connected to the top of the outer side of the processing and fixing barrel 1. The operating combination ring 207 is rotatably connected to the inner side of the clamping thread ring 206. The bottom end of the operating combination ring 207 is attached to the top end of the processing and fixing barrel 1 to achieve alignment support and load-bearing fixation. A plurality of limiting support boxes 208 are welded equidistantly at the bottom end of the operating combination ring 207. A plurality of pressing springs 209 are welded equidistantly at the top end inside the limiting support boxes 208. The bottom ends of the plurality of pressing springs 209 are welded to the downward pressing processing box 210. The downward pressing processing box 210 is slidably sleeved with the limiting support box 208. The bottom end of the anti-slip processing pad 211 is attached to the top end of the fixing support disk 203. The cross section of the anti-slip processing pad 211 is arc-shaped, making it stable when fixing and clamping and limiting the fixing support disk 203, and reducing the shaking of the equipment during use. The anti-slip processing pad 211 is adhered to the bottom end of the downward pressing processing box 210;

[0044] A number of inner hole damping blocks 212 are welded at equal intervals on the outer side end of the engaging thread ring 206. A combined processing column 213 is sleeved inside the inner hole damping block 212. A limiting and fitting frame 214 is welded at the top ends of multiple combined processing columns 213. The bottom end of the limiting and fitting frame 214 is engaged and connected with the top end of the engaging thread ring 206 to achieve stable connection and limitation. A engaging limit cover 215 is rotatably connected inside the limiting and fitting frame 214. A sealing and fixing ring 216 is adhesively bonded to the outer bottom end of the engaging limit cover 215. The side ends of the sealing and fixing ring 216 are respectively in contact with the side ends of the limiting and fitting frame 214 and the operating combined ring 207 to ensure the stability of sealing and limitation.

[0045] A number of exhaust fixing pipes 217 penetrate through the top end of the engaging limit cover 215 at equal intervals. One end of multiple exhaust fixing pipes 217 is penetrated and connected with a filtering and purifying barrel 218. The filtering and purifying barrel 218 is clamped to the top end of the engaging limit cover 215. An inlet and outlet operating pipe 219 is penetrated and connected to the top end of the filtering and purifying barrel 218.

[0046] The processing fixing barrel 1 is provided with a matching component 3;

[0047] The matching component 3 includes a limiting magnetic ring 301, a connecting and fitting frame 302, a counterweight 303, a supporting and buffering block 304, a permanent magnetic ring 305, a connecting magnetic ring 306, an isolation net plate 307, a special-shaped air inlet hole 308 and a porous sealing ring 309;

[0048] A limiting magnetic ring 301 is sleeved on the outer side end of the processing fixing barrel 1. A connecting and fitting frame 302 is rotatably connected to the top end of the limiting magnetic ring 301. A number of counterweights 303 are sleeved inside the connecting and fitting frame 302 at equal intervals. The longitudinal section of the counterweight 303 is T-shaped. Adjacent two counterweights 303 are sleeved with each other to achieve stable counterweight adjustment. A number of supporting and buffering blocks 304 are adhesively bonded to the inner side end of the connecting and fitting frame 302 at equal intervals.

[0049] A permanent magnetic ring 305 is fixed in the middle of the inner side of the engaging limit cover 215. A connecting magnetic ring 306 is magnetically attracted and connected to the bottom end of the permanent magnetic ring 305. Isolation net plates 307 are fixed at the top and bottom of the inner side of the connecting magnetic ring 306. The outer diameter of the connecting magnetic ring 306 is equal to the inner diameter of the engaging limit cover 215. There are two isolation net plates 307 to achieve integrated isolation and ensure the stability of sealing and limitation and sealing and isolation. A special-shaped air inlet hole 308 is opened at the top end of the inner side of the engaging limit cover 215. A porous sealing ring 309 is rotatably connected to the top of the inner side of the special-shaped air inlet hole 308.

[0050] The working principle and usage process of the present invention: When culturing an organism, the operator drips the biological liquid to be cultured onto the inner side of the fixed support plate 203, places the fixed support plate 203 into the processing and fixing barrel 1, and moves the insertion connection port 204 at the side end of the fixed support plate 203 downward along the clamping and restricting column 202, and places the fixed support plate 203 on the top of the restricting support ring 201 to achieve the placement of the fixed support plate 203;

[0051] Place the limit support box 208 and the downward pressure processing box 210 into the processing and fixing barrel 1, and make the bottom end of the operation combination ring 207 fit with the top end of the processing and fixing barrel 1. By rotating the clamping thread ring 206, use the clamping thread groove 205 to connect and combine the clamping thread ring 206 and the processing and fixing barrel 1, and fix the operation combination ring 207 to the top end of the processing and fixing barrel 1. Drive the downward pressure processing box 210 and the anti-slip processing pad 211 to move downward along the limit support box 208 to the top of the fixed support plate 203 by the pressing spring 209, so as to perform a pressing process on the fixed support plate 203 and ensure the stability of the placement of the fixed support plate 203;

[0052] Magnetically fix the connecting magnetic ring 306 through the permanent magnetic ring 305, put the isolation net plate 307 into the inner side of the clamping limit cover 215, put the combined processing column 213 into the inner hole damping block 212, fix the limit matching frame 214 and the clamping limit cover 215 to the top end of the processing and fixing barrel 1, seal and restrict the operation combination ring 207, the processing and fixing barrel 1 and the clamping limit cover 215 through the sealing fixing ring 216, rotate the connecting matching frame 302 to switch the position of the counterweight, magnetically clamp the connecting matching frame 302 by the limit magnetic ring 301, and place the counterweight block 303 into the inner side of the connecting matching frame 302 according to the requirements to achieve counterweight processing and ensure the overall flatness and the stability of the counterweight during processing;

[0053] During the process of culturing the organism in the culture dish, inject gas into the filtering and purifying barrel 218 through the inlet and outlet operation pipe 219. The gas enters the inner side of the processing and fixing barrel 1 along the isolation water in the filtering and purifying barrel 218 through the exhaust fixing pipe 217 to adjust the internal gas environment, and the internal air can enter the inner side of the filtering and purifying barrel 218 along the exhaust fixing pipe 217 to achieve inlet and outlet gas processing and ensure the gas stability of the culture environment. By rotating the porous sealing ring 309, adjust the inlet and outlet gas size of the special-shaped inlet hole 308 to achieve inlet and outlet gas adjustment and ensure the stability of inlet and outlet gas;

[0054] Among them, in this application, the 35mm glass-bottom confocal culture dish has a glass diameter of 15mm, is circular, and has a standard size. The dish body and lid are made of high-quality polystyrene (PS) material, and the glass bottom is made of high-transparency borosilicate glass. The inner surface of the glass-bottom dish is treated with TC. The glass-bottom dish combines the convenience of a 35mm culture dish and the imaging advantages of a cover glass, and is suitable for observation scenarios that require high magnification, such as cell observation experiments using a laser confocal microscope, fluorescence microscope, live cell imaging, phase contrast microscope, differential interference contrast microscope, fluorescence in situ hybridization technique (FISH), etc., and can easily achieve high-quality imaging;

[0055] Product specifications: The lid diameter is 33.61mm, the lid height is 6.18mm, the glass bottom diameter is 15mm, and the glass bottom height is 10mm;

[0056] Durable and reliable material: The dish body and lid are made of high-quality polystyrene (PS) material, and the bottom surface of the culture dish is treated with TC, which is suitable for cell culture;

[0057] High optical performance: The glass bottom is made of high-transparency borosilicate glass, with high bottom flatness. When paired with a high-resolution microscope for observation and photography, the field of view is clearer;

[0058] Cell-free toxicity: It is bonded with the culture dish using a shadowless glue. The glass bottom is firmly combined with the culture dish and is non-toxic to cells;

[0059] Rigorously packaged: Produced in a dust-free workshop, sterilized by gamma irradiation, and independently aseptically packaged, which is simple and convenient to use;

[0060] High cleanliness: Sterile, enzyme-free, pyrogen-free, and endotoxin-free.

[0061] Finally, it should be noted that the above are only preferred examples of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An intelligent confocal culture dish with adjustable counterweight, comprising a processing fixed barrel (1), characterized in that: The processing fixed barrel (1) is provided with an operating component (2); The operating assembly (2) comprises a limiting support ring (201); A limiting support ring (201) is installed inside the treatment fixed barrel (1), a plurality of engaging limiting columns (202) are welded at equal distances on the top of the limiting support ring (201), a fixed support plate (203) is sleeved on the top of the limiting support ring (201), and a plurality of insertion connection ports (204) are opened at equal distances on the top of the fixed support plate (203); The outer end of the treatment fixed barrel (1) is provided with a locking thread groove (205); the outer top of the treatment fixed barrel (1) is connected with a locking thread ring (206) through a thread; the inner side of the locking thread ring (206) is rotatably connected with an operating assembly ring (207); a plurality of limit support boxes (208) are equidistantly welded to the bottom end of the operating assembly ring (207); a plurality of pressing springs (209) are equidistantly welded to the top end of the inner side of the limit support box (208); a plurality of pressing springs (209) are welded to the bottom ends of the plurality of pressing springs (209); and a non-slip treatment pad (211) is bonded to the bottom end of the pressing treatment box (210); A plurality of inner hole damping blocks (212) are welded at equal distances on the outer end of the locking threaded ring (206), a combined processing column (213) is sleeved on the inner side of the inner hole damping block (212), and a limiting matching frame (214) is welded on the top end of the plurality of combined processing columns (213).

2. The intelligent confocal culture dish with adjustable weight according to claim 1, characterized in that: The engaging limiting column (202) is sleeved on the inner side of the insertion connection port (204), and the longitudinal section of the fixed support plate (203) is T-shaped.

3. The intelligent confocal culture dish with adjustable weight according to claim 1, characterized in that: The top of the side end of the fixed support plate (203) fits with the inner side end of the processing fixed barrel (1), and the bottom end of the operating assembly ring (207) fits with the top end of the processing fixed barrel (1).

4. The intelligent confocal culture dish with adjustable weight according to claim 1, characterized in that: The downward pressure processing box (210) is slidably fitted with the limiting support box (208), and the bottom end of the anti-slip processing pad (211) is fitted with the top end of the fixed support plate (203).

5. The intelligent confocal culture dish with adjustable weight according to claim 1, characterized in that: The cross section of the anti-slip treatment pad (211) is arc-shaped, and the bottom end of the limiting matching frame (214) is engaged and connected with the top end of the engaging threaded ring (206).

6. The intelligent confocal culture dish with adjustable counterweight according to claim 1, characterized in that: The inner side of the limiting matching frame (214) is rotatably connected with a locking limit cover (215), and the outer bottom end of the locking limit cover (215) is bonded with a sealing fixing ring (216); A plurality of exhaust fixed pipes (217) are equidistantly connected through the top of the locking limit cover (215), one end of each of the plurality of exhaust fixed pipes (217) is connected through a filter purification barrel (218), and an inlet and outlet operation pipe (219) is connected through the top of the filter purification barrel (218).

7. The intelligent confocal culture dish with adjustable counterweight according to claim 6, characterized in that: The filtering and purifying barrel (218) is snap-fitted to the top end of the snap-fit ​​limiting cover (215), and the side ends of the sealing fixing ring (216) are respectively fitted with the side ends of the limiting matching frame (214) and the side ends of the operating combination ring (207).

8. The intelligent confocal culture dish with adjustable weight according to claim 6, characterized in that: The treatment fixed barrel (1) is provided with a matching component (3); The mating component (3) comprises a limiting magnetic ring (301); The outer end of the treatment fixed barrel (1) is sleeved with a limiting magnetic ring (301), the top end of the limiting magnetic ring (301) is rotatably connected to a connecting and matching frame (302), the inner side of the connecting and matching frame (302) is sleeved with a plurality of balancing weights (303) at equal distances, and the inner end of the connecting and matching frame (302) is bonded with a plurality of supporting buffer blocks (304) at equal distances; A permanent magnetic ring (305) is fixed to the middle of the inner side of the locking limit cover (215); a connecting magnetic ring (306) is magnetically connected to the bottom end of the permanent magnetic ring (305); isolation mesh plates (307) are fixed to the inner top and bottom of the connecting magnetic ring (306); a special-shaped air inlet hole (308) is opened at the inner top of the locking limit cover (215); and a porous sealing ring (309) is rotatably connected to the inner top of the special-shaped air inlet hole (308).

9. The intelligent confocal culture dish with adjustable counterweight according to claim 8, characterized in that: The outer diameter of the connecting magnetic ring (306) is equal to the inner diameter of the locking limit cover (215), and there are two isolation mesh plates (307).

10. The intelligent confocal culture dish with adjustable weight according to claim 8, characterized in that: The longitudinal section of the counterweight block (303) is T-shaped, and two adjacent counterweight blocks (303) are fitted into each other.

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