Rapid sampling device for gene detection and sampling method thereof

Abstract

The invention discloses a gene detection rapid sampling device and a sampling method thereof, and belongs to the technical field of gene detection sampling, the gene detection rapid sampling device comprises a sampling table, the surfaces of the two end parts of the sampling table are respectively provided with a sampling frame and a sample lifting frame; the sliding unit is slidably connected to the sampling table, and a pipette is mounted at the end of the sliding unit; the rotating unit is rotationally connected to the sampling table and is connected with the sliding unit; the replacing units are rotationally connected to the sampling table, the number of the replacing units is two, and the two replacing units are slidably connected into the two steering units; and the grabbing unit is connected to the two groups of replacing units and is connected with the surface of the pipette in a clamping manner. A new pipette is placed in one set of grabbing unit away from the sliding unit, the replacing units are started, the two sets of replacing units can drive the two sets of grabbing units to turn over under the cooperation of the steering unit, and therefore the two sets of grabbing units can automatically grab and replace the used pipette, and the automatic pipette replacing device has the advantage of being high in automation degree.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection sampling, and specifically relates to a gene detection rapid sampling device and a sampling method thereof. Background Art

[0002] Chinese Patent (Publication No.: CN118726069A) discloses a gene detection rapid sampling device and a sampling method thereof, including a pipette and a fixing plate. A second telescopic rod and a third telescopic rod are fixed on the fixing plate through a connecting plate. The second telescopic rod is used to push the liquid suction button of the pipette, and the third telescopic rod is used to push the gun head ejection button of the pipette; the fixing plate is connected to a Y-axis driving mechanism through a Z-axis driving mechanism, the Y-axis driving mechanism is connected to a workbench through an X-axis driving mechanism, and a test tube rack, a gun head box, a waste box, and an extraction box are arranged on the workbench. This patent has the advantages of automatically moving the sample in the sampling tube to the extraction box of the automatic extractor, with high automation and high efficiency, and solves the problem in the prior art that staff need to use a pipette to move the blood samples in the sampling bottles to the extraction box of the automatic extractor one by one, resulting in low manual operation efficiency.

[0003] The gene detection rapid sampling device in the above technical solution still has the following defects when in use: Since each used pipette is disposable, it needs to be replaced after use. In the prior art, the used pipette usually needs to be replaced manually, with low automation, which reduces the efficiency of gene detection. Therefore, a gene detection rapid sampling device and a sampling method thereof are needed to solve the above problems. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the purpose of the embodiments of the present invention is to provide a gene detection rapid sampling device and a sampling method thereof to solve the problems in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solutions:

[0006] A gene detection rapid sampling device and a sampling method thereof, including:

[0007] A sampling table, on the surfaces of both ends of which a sampling rack and a sample lifting rack are respectively installed, and a sampling bottle and an extraction box are respectively placed on the sampling rack and the sample lifting rack;

[0008] A sliding unit, slidably connected to the sampling table, with a pipette installed at the end, for driving the pipette to move up and down;

[0009] A rotating unit, rotatably connected to the sampling table, and connected to the sliding unit, for cooperating with the sliding unit to realize the rotation and intermittent lifting of the pipette;

[0010] The replacement unit is rotatably connected to the sampling table. There are two groups of the replacement units installed, and the two groups of the replacement units are slidably connected within two groups of steering units. The replacement unit is used to cooperate with the steering unit to realize the replacement of the pipette.

[0011] The grasping unit is connected to the two groups of replacement units and is snap-connected to the surface of the pipette, and is used to grasp and place the pipette.

[0012] As a preferred technical solution of the present invention, the sliding unit includes: a turntable rotatably connected to the upper side of the sampling table, with at least eight groups of sliding rods slidably connected inside. The end of the sliding rod is connected to the end of the pipette through a vacuum chuck; a bracket fixed on the surface of the sliding rod, and the bracket is connected to the turntable through an elastic member.

[0013] As a preferred technical solution of the present invention, the rotating unit includes: a first bracket fixed on the sampling table, with a base installed at the end. The turntable is rotatably connected to the surface of the base; a driving member installed on the side of the base, and the center of the turntable is connected to the output end of the driving member; a second bracket fixed on the sampling table, with a fixing frame installed at the end. The surface of the fixing frame is slidably connected to the end of the sliding rod; a convex-shaped frame connected to the side of the fixing frame. There are two groups of the convex-shaped frames, and the two groups of convex-shaped frames are respectively located on the upper sides of the ends of the sampling frame and the sample-lifting frame, and the sliding rod is slidably connected to the surface of the convex-shaped frame.

[0014] As a preferred technical solution of the present invention, the replacement unit includes: a first delivery box fixed on the upper side of the sampling table; a second delivery box fixed on the upper side of the sampling table and located on the side of the first delivery box; a replacement frame fixed on the surface of the sampling table, with a driver installed on the side; a driving shaft rotatably connected to the replacement frame, and the end is connected to the output end of the driver; a gear installed on the surface of the driving shaft; a rack slidably connected to the surface of the replacement frame. There are two groups of the racks, and both groups of the racks are meshed with the gear; a sliding member fixed on the sides of the two groups of the racks; a guiding rod connected to the surface of the replacement frame, and the two sliding members are respectively slidably connected to the surfaces of the two guiding rods; a rotating shaft rotatably connected to the two sliding members, and rotating plates are installed on both groups of the rotating shafts.

[0015] As a preferred technical solution of the present invention, the steering unit includes: a connecting frame connected to the side of the replacement frame, with a first sliding groove and a second sliding groove opened on the surface. The rotating shaft is located directly above the first sliding groove and the second sliding groove; a semi-circular groove opened on the connecting frame, and the two ends are respectively communicated with the ends of the first sliding groove and the second sliding groove; a rotating frame installed at the end of the rotating shaft away from the sliding member, and the length of the rotating frame is equal to the radius length of the circle where the semi-circular groove is located; a sliding column fixed on the side of the rotating frame, and the end is slidably connected to the first sliding groove, the second sliding groove, and the semi-circular groove.

[0016] As a preferred technical solution of the present invention, the grasping unit includes: a fixed block connected to the surface of the rotating plate, with a slider connected to the side through a telescopic rod; a rotating pin connected to the slider, and grasping frames are respectively rotatably connected to both ends thereof; a grasping groove is opened at the end of the grasping frame; a V-shaped frame is opened on the surface of the fixed block; sliding columns are fixed on the sides of the two grasping frames away from the grasping groove, and the ends of the two sliding columns are slidably connected in the V-shaped frame.

[0017] A rapid sampling method for gene detection includes the following steps:

[0018] Step 1: Place the sampling bottle and the extraction box on the sampling rack and the sample extraction rack respectively, and install the pipette on the end of the sliding rod through a vacuum chuck.

[0019] Step 2: Turn on the driving member. The output end of the driving member can drive the turntable to rotate on the surface of the base, so that the sliding rod drives the pipette at its end to descend into the sampling bottle and the extraction box, and the blood sample in the sampling bottle is transferred into the extraction box through the pipette.

[0020] Step 3: Put a new pipette into one of the grasping units far from the sliding rod, turn on the driver, so that the two racks slide in opposite directions, and the two rotating plates will drive the grasping unit to flip 180 degrees.

[0021] Step 4: When the rotating plate drives the grasping unit to flip to the lower side of the sliding rod, turn on the telescopic rod, so that the slider slides away from the fixed block, and then the two grasping frames will slide away from the fixed block and also move closer to each other until the two grasping frames drive the grasping groove to grasp the pipette at the lower end of the sliding rod.

[0022] Step 5: When the rotating plate drives the pipette to flip into the first delivery box through the grasping unit, turn on the telescopic rod again, so that the slider slides towards the fixed block. At this time, the two grasping frames will slide towards the fixed block and also spread out, so that the two grasping frames can place the used pipette in the first delivery box through the grasping groove, realizing the placement of the pipette.

[0023] Compared with the prior art, the embodiment of the present invention has the following beneficial effects: In the present invention, the sampling bottle and the extraction box are respectively placed on the sampling rack and the sample extraction rack, and the pipette is installed on the end of the sliding unit through a vacuum chuck. Turn on the rotating unit, and the sliding unit will rotate and periodically slide downward under the action of the rotating unit. When the sliding unit drives the pipette at its end to descend into the sampling bottle and the extraction box, the blood sample in the sampling bottle is transferred into the extraction box through the pipette.

[0024] In the embodiment of the present invention, compared with the prior art that requires manual replacement of the used pipette, by setting a replacement unit and a grasping unit, a new pipette is placed in a set of grasping units far from the sliding unit. The replacement unit is activated, and with the cooperation of the steering unit, the two sets of replacement units will drive the two sets of grasping units to flip 180 degrees. Thus, the two sets of grasping units can automatically grasp and replace the used pipette, which has the advantages of high automation and high gene detection efficiency.

[0025] To more clearly elaborate on the structural features and functions of the present invention, the present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the gene detection rapid sampling device and its sampling method provided by the embodiment of the present invention.

[0027] Figure 2 It is a schematic diagram of the structure of the rotation unit provided in the embodiment of the present invention.

[0028] Figure 3 It is a bottom view of the fixing frame provided in the embodiment of the present invention.

[0029] Figure 4 For Figure 2 The partial enlarged view of part A in

[0030] Figure 5 It is a schematic diagram of the structure of the steering unit provided in the embodiment of the present invention.

[0031] Figure 6 It is a schematic diagram of the structure of the grasping unit provided in the embodiment of the present invention.

[0032] Reference numerals: 1, sampling table; 10, pipette; 11, sampling rack; 111, sampling bottle; 12, sample lifting rack; 121, extraction box; 2, sliding unit; 21, turntable; 22, sliding rod; 23, support frame; 24, elastic member; 3, rotation unit; 31, first support; 32, base; 33, driving member; 34, second support; 35, fixing frame; 36, convex frame; 4, replacement unit; 41, first conveying box; 42, second conveying box; 43, replacement rack; 431, driver; 44, driving shaft; 441, gear; 45, rack; 46, sliding member; 47, guiding rod; 48, rotating shaft; 49, rotating plate; 5, grasping unit; 51, fixing block; 52, telescopic rod; 53, slider; 531, rotating pin; 54, grasping rack; 55, grasping groove; 56, V-shaped rack; 57, sliding column; 6, steering unit; 61, connecting rack; 62, first chute; 63, second chute; 64, semi-circular groove; 65, rotating rack; 66, sliding column. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0033] In order to make the objectives, technical solutions and advantages of the present invention more clear and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0034] The following describes the specific implementation of the present invention in detail with reference to specific embodiments.

[0035] See Figures 1 to 6 , a rapid sampling device for gene detection, comprising:

[0036] A sampling table 1, on the surfaces of both ends of the sampling table 1, a sampling rack 11 and a sample lifting rack 12 are respectively installed, and a sampling bottle 111 and an extraction box 121 are respectively placed on the sampling rack 11 and the sample lifting rack 12;

[0037] A sliding unit 2, slidably connected to the sampling table 1, and a pipette 10 is installed at the end for driving the pipette 10 to lift and lower;

[0038] A rotating unit 3, rotatably connected to the sampling table 1, and connected to the sliding unit 2, for cooperating with the sliding unit 2 to realize the rotation and intermittent lifting of the pipette 10;

[0039] A replacement unit 4, rotatably connected to the sampling table 1, there are two groups of the replacement unit 4, and the two groups of the replacement unit 4 are slidably connected in two groups of steering units 6, and the replacement unit 4 is used to cooperate with the steering unit 6 to realize the replacement of the pipette 10;

[0040] A grasping unit 5, connected to the two groups of replacement units 4, and snap-fitted to the surface of the pipette 10, for grasping and placing the pipette 10.

[0041] In an embodiment of the present invention, the sampling bottle 111 and the extraction box 121 are respectively placed on the sampling rack 11 and the sample lifting rack 12, and the pipette 10 is installed at the end of the sliding unit 2 through a vacuum chuck. The rotating unit 3 is turned on, and the sliding unit 2 will rotate and periodically slide downward under the action of the rotating unit 3. When the sliding unit 2 drives the pipette 10 at its end to descend into the sampling bottle 111 and the extraction box 121, the blood sample in the sampling bottle 111 can be transferred into the extraction box 121 through the pipette 10. A new pipette 10 is placed in a group of grasping units 5 far from the sliding unit 2. The replacement unit 4 is turned on, and under the cooperation of the steering unit 6, the two groups of replacement units 4 will drive the two groups of grasping units 5 to flip 180 degrees, so that the two groups of grasping units 5 can automatically grasp and replace the used pipette 10.

[0042] In an embodiment of the present invention, as Figure 3As shown, the sliding unit 2 includes:

[0043] A turntable 21, rotatably connected to the upper side of the sampling table 1, with at least eight groups of sliding rods 22 slidably connected inside. The end of the sliding rod 22 is connected to the end of the pipette 10 through a vacuum chuck.

[0044] A support frame 23, fixed to the surface of the sliding rod 22, and connected to the turntable 21 through an elastic member 24.

[0045] In this embodiment, when using the pipette 10 to sample the blood in the sampling bottle 111, first place the sampling bottle 111 and the extraction box 121 on the sampling rack 11 and the sample extraction rack 12 respectively, and install the pipette 10 on the end of the sliding rod 22 through a vacuum chuck. Turn on the rotating unit 3, and the rotating unit 3 can drive the turntable 21 to rotate. When the turntable 21 rotates, it can drive the pipette 10 at its end to rotate synchronously through the sliding rod 22. And when the pipette 10 rotates to the upper side of the sampling bottle 111 or the extraction box 121, the sliding rod 22 will slide downward in the turntable 21 under the action of the rotating unit 3 until the sliding rod 22 drives the pipette 10 at its end to descend into the sampling bottle 111 and the extraction box 121 respectively.

[0046] When the sliding rod 22 slides downward, it can drive the support frame 23 to compress the elastic member 24. The elastic member 24 can be a sampling spring or elastic rubber, which is not uniquely defined here. When the pipette 10 descends into the sampling bottle 111, the pipette 10 can sample the blood in the sampling bottle 111. When the pipette 10 descends into the extraction box 121, the pipette 10 can extract the sampled blood into the extraction box 121, so as to transfer the blood sample in the sampling bottle 111 to the extraction box 121 through the pipette 10.

[0047] In an embodiment of the present invention, as Figure 2 and Figure 3 shown, the rotating unit 3 includes:

[0048] A first bracket 31, fixed on the sampling table 1, with a base 32 installed at its end. The turntable 21 is rotatably connected to the surface of the base 32.

[0049] A driving member 33, installed on the side of the base 32, and the center of the turntable 21 is connected to the output end of the driving member 33.

[0050] A second bracket 34, fixed on the sampling table 1, with a fixing frame 35 installed at its end. The surface of the fixing frame 35 is slidably connected to the end of the sliding rod 22.

[0051] The convex frame 36 is connected to the side of the fixed frame 35. Two groups of convex frames 36 are installed. The two groups of convex frames 36 are respectively located on the upper sides of the ends of the sampling frame 11 and the sample lifting frame 12, and the sliding rod 22 is slidably connected to the surface of the convex frame 36.

[0052] In this embodiment, the driving member 33 is turned on, and the output end of the driving member 33 can drive the turntable 21 to rotate on the surface of the base 32. When the turntable 21 rotates, it can drive the pipette gun 10 at its end to rotate through the slide rod 22, and the turntable 21 can drive the end of the slide rod 22 to slide on the surface of the fixed frame 35. When the end of the slide rod 22 slides to the surface of the convex frame 36, the slide rod 22 will slide downward in the turntable 21 under the pressure of the convex part of the convex frame 36. When the slide rod 22 slides downward, it can drive the support frame 23 to compress the elastic member 24 until the slide rod 22 drives the pipette gun 10 at its end to descend into the sampling bottle 111 and the extraction box 121, thereby realizing the blood sample in the sampling bottle 111 being moved to the extraction box 121 through the pipette gun 10.

[0053] When the upper end of the slide rod 22 slides to the surface of the convex frame 36 to be separated from the convex frame 36, the support frame 23 will push the slide rod 22 to slide upward in the turntable 21 under the elastic force of the elastic member 24, so that the slide rod 22 can drive the pipette gun 10 to be separated from the sampling bottle 111 and the extraction box 121.

[0054] In one embodiment of the present invention, Figure 4 and Figure 5 As shown, the replacement unit 4 includes:

[0055] The first transport box 41 is fixed on the upper side of the sampling table 1;

[0056] The second transport box 42 is fixed on the upper side of the sampling platform 1 and is located on the side of the first transport box 41;

[0057] The replacement rack 43 is fixed on the surface of the sampling table 1, and a driver 431 is installed on the side;

[0058] A driving shaft 44 is rotatably connected to the replacement frame 43, and an end portion is connected to an output end of the driver 431;

[0059] Gear 441, mounted on the surface of the driving shaft 44;

[0060] The rack 45 is slidably connected to the surface of the replacement frame 43. Two groups of the racks 45 are installed, and both groups of racks 45 are meshed and connected with the gear 441.

[0061] Sliding members 46 are fixed to the sides of the two sets of racks 45;

[0062] The guide rod 47 is connected to the surface of the replacement frame 43, and two sets of sliding members 46 are respectively slidably connected to the surfaces of the two guide rods 47;

[0063] The rotating shaft 48 is rotatably connected to the two sets of sliding members 46, and rotating plates 49 are installed on both of the two rotating shafts 48.

[0064] In this embodiment, when the driver 431 is turned on, the output end of the driver 431 can drive the drive shaft 44 to rotate within the replacement frame 43, and when the drive shaft 44 rotates, the gear 441 on its surface can be driven to rotate synchronously. Since the gear 441 is meshed with the two racks 45 respectively, the two racks 45 will first move closer to each other and then move away from each other. When the racks 45 slide, they can drive the sliding members 46 to slide on the surfaces of the guide rods 47, so that the guide rods 47 and the sliding members 46 can guide and limit the racks 45, improving the stability of the two racks 45 when sliding. When the sliding members 46 slide, they can drive the rotating plates 49 to slide synchronously through the rotating shafts 48, so that the rotating plates 49 can drive the grasping units 5 on their surfaces to grasp the pipette 10.

[0065] In an embodiment of the present invention, as Figure 5 shown, the steering unit 6 includes:

[0066] The connecting frame 61 is connected to the side surface of the replacement frame 43, and a first sliding groove 62 and a second sliding groove 63 are formed on the surface. The rotating shaft 48 is located directly above the first sliding groove 62 and the second sliding groove 63;

[0067] The semi-circular groove 64 is formed in the connecting frame 61, and the two ends are respectively communicated with the ends of the first sliding groove 62 and the second sliding groove 63;

[0068] The rotating frame 65 is installed at the end of the rotating shaft 48 away from the sliding member 46, and the length of the rotating frame 65 is equal to the radius length of the circle where the semi-circular groove 64 is located;

[0069] The sliding column 66 is fixed to the side surface of the rotating frame 65, and the end is slidably connected in the first sliding groove 62, the second sliding groove 63 and the semi-circular groove 64.

[0070] In this embodiment, as Figure 4As shown, when the drive shaft 44 drives the gear 441 to rotate counterclockwise, under the action of the gear 441 at this time, the left rack 45 will slide to the right, and the right rack 45 will slide to the left. Since the rack 45 will drive the rotating shaft 48 to slide through the sliding member 46, the rotating shaft 48 can drive the sliding column 66 to slide synchronously through the rotating frame 65. At this time, the left sliding member 46 will drive the rotating shaft 48 and the rotating frame 65 to slide to the right, so that this group of rotating frames 65 will drive the sliding column 66 on its surface to slide to the right in the second chute 63 until the sliding column 66 slides into the semi-circular groove 64.

[0071] After the sliding column 66 slides into the semi-circular groove 64, the sliding column 66 will drive the rotating shaft 48 to rotate on the sliding member 46 through the rotating frame 65 under the action of the semi-circular groove 64. Since the length of the rotating frame 65 is equal to the radius length of the circle where the semi-circular groove 64 is located, when the sliding column 66 slides to the midpoint position of the semi-circular groove 64, at this time, the rotating frame 65 will drive the rotating shaft 48 to rotate 90 degrees on the sliding member 46 under the action of the sliding column 66 and the semi-circular groove 64.

[0072] When the sliding member 46 continues to drive the rotating frame 65 and the sliding column 66 to slide through the rotating shaft 48, at this time, the sliding column 66 will slide into the other half of the semi-circular groove 64. At the same time, the rotating frame 65 will continue to drive the rotating shaft 48 to rotate on the sliding member 46 under the action of the semi-circular groove 64 and the sliding column 66 until the sliding column 66 slides into the first chute 62. At this time, the rotating frame 65 drives the rotating shaft 48 to rotate 180 degrees on the sliding member 46 under the action of the sliding column 66 and the semi-circular groove 64, that is to say, the rotating shaft 48 drives the rotating plate 49 at its end to turn around, so that the rotating plate 49 drives the grasping unit 5 on its surface to turn over 180 degrees.

[0073] When the drive shaft 44 drives the gear 441 to rotate clockwise, the same as the above working process, the rotating frame 65 will also drive the rotating shaft 48 to rotate 180 degrees on the sliding member 46 under the action of the sliding column 66 and the semi-circular groove 64, so that the rotating plate 49 drives the grasping unit 5 to turn over 180 degrees in the reverse direction.

[0074] The grasping unit 5 can grasp the pipette 10. By placing a new pipette 10 in the first delivery box 41 (or the second delivery box 42), the driver 431 is turned on, causing the two racks 45 to slide in opposite directions. Under the action of the steering unit 6, both rotating plates 49 will drive the grasping unit 5 on their surfaces to flip 180 degrees. Therefore, when one of the grasping units 5 moves to the surface of the used pipette 10 at the lower end of the sliding rod 22, this grasping unit 5 can grasp the used pipette 10 at the lower end of the sliding rod 22. The driver 431 is turned on again, and the rotating plate 49 will drive this group of pipettes 10 to flip 180 degrees through the grasping unit 5, so that the grasping unit 5 just places the used pipette 10 in the second delivery box 42. At the same time, the other rotating plate 49 will also drive the grasping unit 5 to flip 180 degrees. Therefore, the other rotating plate 49 will place the new pipette 10 in the first delivery box 41 on the end of the sliding rod 22 through the grasping unit 5, so that the vacuum suction cup at the end of the sliding rod 22 fixes the new pipette 10.

[0075] Therefore, under the action of the steering unit 6, as long as a new pipette 10 is placed in the grasping unit 5 away from the sliding rod 22, the automatic replacement of the used pipette 10 can be realized. The driver 431 and the driving member 33 can adopt a motor or a pneumatic motor, which is not uniquely limited here.

[0076] In an embodiment of the present invention, as Figure 6 shown, the grasping unit 5 includes:

[0077] A fixed block 51, connected to the surface of the rotating plate 49, and a slider 53 is connected to the side surface through a telescopic rod 52;

[0078] A rotating pin 531, connected to the slider 53, and two ends are respectively rotatably connected to a grasping frame 54;

[0079] A grasping groove 55, opened at the end of the grasping frame 54;

[0080] A V-shaped frame 56, opened on the surface of the fixed block 51;

[0081] Sliding columns 57, fixed on the side surfaces of the two grasping frames 54 away from the grasping groove 55, and the ends of the two sliding columns 57 are slidably connected in the V-shaped frame 56.

[0082] In this embodiment, when the telescopic rod 52 is turned on, the telescopic rod 52 can drive the slider 53 to slide along the direction of the telescopic rod 52. When the slider 53 slides, it can drive the two grasping frames 54 on both sides through the rotating pin 531 to slide synchronously. Therefore, the two grasping frames 54 can drive the two sliding columns 57 to slide in the V-shaped frame 56 respectively.

[0083] When the slider 53 slides away from the fixed block 51, the two sets of gripper frames 54 can drive the two sets of sliding columns 57 to slide within the V-shaped frame 56 towards the midpoint position of the V-shaped frame 56. Since the midpoint positions of the V-shaped frame 56 gradually approach each other, the two sets of gripper frames 54 will rotate towards each other on the surface of the rotating pin 531 under the action of the sliding columns 57 and the V-shaped frame 56, so that the gripper frames 54 will drive the gripping grooves 55 to approach each other.

[0084] In summary, when the slider 53 slides away from the fixed block 51, the two sets of gripper frames 54 will not only slide away from the fixed block 51 but also approach each other until the two sets of gripper frames 54 drive the gripping grooves 55 to grip the pipette 10. When the slider 53 slides towards the fixed block 51, it is the opposite of the above working process. At this time, the two sets of gripper frames 54 will not only slide towards the fixed block 51 but also expand, so that the two sets of gripper frames 54 can place the used pipette 10 in the first delivery box 41 (or the second delivery box 42) through the gripping grooves 55, realizing the gripping and placement of the pipette 10.

[0085] A rapid sampling method for gene detection, using the rapid sampling device for gene detection described above, includes the following steps:

[0086] Step 1: Place the sampling bottle 111 and the extraction box 121 on the sampling rack 11 and the sample extraction rack 12 respectively, and install the pipette 10 at the end of the sliding rod 22 through the vacuum chuck.

[0087] Step 2: Turn on the driving member 33. The output end of the driving member 33 can drive the turntable 21 to rotate on the surface of the base 32, so that the sliding rod 22 drives the pipette 10 at its end to descend into the sampling bottle 111 and the extraction box 121, and the blood sample in the sampling bottle 111 is transferred into the extraction box 121 through the pipette 10.

[0088] Step 3: Place a new pipette 10 in one set of gripping units 5 away from the sliding rod 22. Turn on the driver 431 to make the two sets of racks 45 slide in opposite directions, and the two sets of rotating plates 49 will drive the gripping unit 5 to flip 180 degrees.

[0089] Step 4: When the rotating plate 49 drives the gripping unit 5 to flip to the lower side of the sliding rod 22, turn on the telescopic rod 52 to make the slider 53 slide away from the fixed block 51, so that the two sets of gripper frames 54 will not only slide away from the fixed block 51 but also approach each other until the two sets of gripper frames 54 drive the gripping grooves 55 to grip the pipette 10 at the lower end of the sliding rod 22.

[0090] Step Five: When the rotating plate 49 drives the pipette 10 to flip into the first transport box 41 (or the second transport box 42) through the grasping unit 5, the telescopic rod 52 is turned on again, so that the slider 53 slides in the direction close to the fixed block 51. At this time, the two grasping frames 54 will not only slide in the direction close to the fixed block 51, but also unfold from each other. Thus, the two grasping frames 54 can place the used pipette 10 in the first transport box 41 (or the second transport box 42) through the grasping slots 55, realizing the placement of the pipette 10.

[0091] The working principle of the present invention is as follows: When using the pipette 10 to sample the blood in the sampling bottle 111, first place the sampling bottle 111 and the extraction box 121 on the sampling rack 11 and the sample extraction rack 12 respectively, and install the pipette 10 at the end of the sliding rod 22 through the vacuum chuck. Turn on the driving member 33, and the output end of the driving member 33 can drive the turntable 21 to rotate on the surface of the base 32, so that the sliding rod 22 drives the pipette 10 at its end to descend into the sampling bottle 111 and the extraction box 121. Thus, the blood sample in the sampling bottle 111 is transferred into the extraction box 121 through the pipette 10.

[0092] Turn on the driver 431. When the drive shaft 44 drives the gear 441 to rotate counterclockwise, at this time, under the action of the gear 441, the left rack 45 will slide to the right, and the right rack 45 will slide to the left. Thus, the rotating frame 65 will drive the rotating shaft 48 to rotate 180 degrees on the sliding member 46 under the action of the sliding column 66 and the semi-circular groove 64, so that the rotating plate 49 drives the grasping unit 5 to flip 180 degrees in the opposite direction. Therefore, under the action of the steering unit 6, as long as a new pipette 10 is placed in a set of grasping units 5 far from the sliding rod 22, the automatic replacement of the used pipette 10 can be realized.

[0093] Turn on the telescopic rod 52. The telescopic rod 52 can drive the slider 53 to slide along the direction of the telescopic rod 52. When the slider 53 slides in the direction away from the fixed block 51, the two grasping frames 54 will not only slide in the direction away from the fixed block 51, but also approach each other until the two grasping frames 54 drive the grasping slots 55 to grasp the pipette 10. When the slider 53 slides in the direction close to the fixed block 51, at this time, the two grasping frames 54 will not only slide in the direction close to the fixed block 51, but also unfold from each other. Thus, the two grasping frames 54 can place the used pipette 10 in the first transport box 41 (or the second transport box 42) through the grasping slots 55, realizing the grasping and placement of the pipette 10.

[0094] In the description of the present invention, it should be noted that, unless otherwise clearly specified and defined, the terms "installed", "connected" and "coupled" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection or an integral connection; it may be a mechanical connection, an electrical connection or a connection capable of mutual communication; it may be directly connected or indirectly connected through an intermediate medium, and it may be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0095] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A rapid sampling device for gene detection, characterized in that: The gene detection rapid sampling device and the sampling method thereof include: A sampling platform (1), wherein a sampling frame (11) and a sample lifting frame (12) are respectively mounted on the surfaces of both ends of the sampling platform (1), and a sampling bottle (111) and an extraction box (121) are respectively placed on the sampling frame (11) and the sample lifting frame (12); A sliding unit (2) is slidably connected to the sampling platform (1), and a liquid transfer gun (10) is installed at the end thereof, and is used to drive the liquid transfer gun (10) to be raised and lowered; A rotating unit (3) is rotatably connected to the sampling platform (1) and connected to the sliding unit (2), and is used to cooperate with the sliding unit (2) to realize the rotation and intermittent lifting of the pipette gun (10); A replacement unit (4) is rotatably connected to the sampling platform (1), wherein two groups of the replacement unit (4) are installed, and the two groups of the replacement unit (4) are slidably connected in two groups of steering units (6), and the replacement unit (4) is used to cooperate with the steering unit (6) to achieve the replacement of the pipette gun (10); The grabbing unit (5) is connected to the two groups of replacement units (4) and is snap-connected to the surface of the liquid transfer gun (10) for grabbing and placing the liquid transfer gun (10).

2. The rapid sampling device for gene detection according to claim 1, characterized in that: The sliding unit (2) comprises: A turntable (21) is rotatably connected to the upper side of the sampling platform (1), and at least eight sets of sliding rods (22) are slidably connected inside, and the ends of the sliding rods (22) are connected to the ends of the pipette gun (10) through a vacuum suction cup; The support frame (23) is fixed on the surface of the slide bar (22), and the support frame (23) and the rotating disk (21) are connected via an elastic member (24).

3. The rapid sampling device for gene detection according to claim 2, characterized in that: The rotating unit (3) comprises: A first bracket (31) is fixed on the sampling table (1), and a base (32) is installed at the end thereof, and the rotating disk (21) is rotatably connected to the surface of the base (32); A driving member (33) is installed on the side of the base (32), and the center of the rotating disk (21) is connected to the output end of the driving member (33); A second bracket (34) is fixed on the sampling table (1), and a fixing frame (35) is installed at the end thereof, and the surface of the fixing frame (35) is slidably connected to the end of the sliding rod (22); The convex frame (36) is connected to the side of the fixed frame (35), and the convex frame (36) is installed in two groups. The two groups of convex frames (36) are respectively located on the upper sides of the ends of the sampling frame (11) and the sample lifting frame (12), and the sliding rod (22) is slidably connected to the surface of the convex frame (36).

4. The rapid sampling device for gene detection according to claim 1, characterized in that: The replacement unit (4) comprises: A first transport box (41) is fixed on the upper side of the sampling platform (1); A second conveying box (42) is fixed on the upper side of the sampling platform (1) and is located on the side of the first conveying box (41); A replacement rack (43) is fixed on the surface of the sampling table (1) and has a driver (431) installed on the side thereof; A driving shaft (44) is rotatably connected to the replacement frame (43), and an end portion is connected to an output end of the driver (431); A gear (441) mounted on the surface of the driving shaft (44); A rack (45) is slidably connected to the surface of the replacement frame (43), and two groups of racks (45) are installed, and both groups of racks (45) are meshedly connected with the gear (441); A sliding member (46) is fixed on the sides of the two sets of racks (45); A guide rod (47) is connected to the surface of the replacement frame (43), and two sets of sliding members (46) are respectively slidably connected to the surfaces of the two sets of guide rods (47); The rotating shaft (48) is rotatably connected to the two sets of sliding members (46), and the two sets of rotating shafts (48) are both equipped with rotating plates (49).

5. The rapid sampling device for gene detection according to claim 4, characterized in that: The steering unit (6) comprises: A connecting frame (61) is connected to the side of the replacement frame (43), and a first slide groove (62) and a second slide groove (63) are provided on the surface thereof, and the rotating shaft (48) is located directly above the first slide groove (62) and the second slide groove (63); A semicircular groove (64) is provided on the connecting frame (61), and its two ends are respectively connected to the ends of the first slide groove (62) and the second slide groove (63); A rotating frame (65) is mounted on the end of the rotating shaft (48) away from the sliding member (46), and the length of the rotating frame (65) is equal to the radius of the circle where the semicircular groove (64) is located; The sliding column (66) is fixed on the side of the rotating frame (65), and the end portion is slidably connected in the first sliding groove (62), the second sliding groove (63) and the semicircular groove (64).

6. The rapid sampling device for gene detection according to claim 4, characterized in that: The grabbing unit (5) comprises: A fixed block (51) is connected to the surface of the rotating plate (49), and a sliding block (53) is connected to the side thereof via a telescopic rod (52); A rotating pin (531) is connected to the slider (53), and its two ends are respectively rotatably connected to a grabbing frame (54); A grabbing groove (55) is provided at the end of the grabbing frame (54); A V-shaped frame (56) is provided on the surface of the fixed block (51); The sliding posts (57) are fixed on the sides of the two groups of grabbing frames (54) away from the grabbing grooves (55), and the ends of the two groups of sliding posts (57) are slidably connected in the V-shaped frame (56).

7. A gene detection rapid sampling method, using a gene detection rapid sampling device as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: Step 1: Place the sampling bottle (111) and the extraction box (121) on the sampling rack (11) and the sample extraction rack (12) respectively, and install the pipette gun (10) on the end of the sliding rod (22) through the vacuum suction cup; Step 2: Turn on the driving member (33), and the output end of the driving member (33) can drive the turntable (21) to rotate on the surface of the base (32), so that the sliding rod (22) drives the pipette gun (10) at its end to descend into the sampling bottle (111) and the extraction box (121), so that the blood sample in the sampling bottle (111) is moved into the extraction box (121) through the pipette gun (10); Step 3: Place a new pipette (10) in a group of grabbing units (5) away from the slide bar (22), turn on the driver (431), so that the two groups of racks (45) slide in opposite directions, and the two groups of rotating plates (49) drive the grabbing units (5) to flip 180 degrees; Step 4: When the rotating plate (49) drives the grabbing unit (5) to flip to the lower side of the slide bar (22), the telescopic rod (52) is opened, so that the slider (53) slides in the direction away from the fixed block (51), so that the two groups of grabbing frames (54) will not only slide in the direction away from the fixed block (51), but also move closer to each other, until the two groups of grabbing frames (54) drive the grabbing groove (55) to grab the pipette gun (10) at the lower end of the slide bar (22); Step 5: When the rotating plate (49) drives the pipette (10) to flip into the first conveying box (41) through the grabbing unit (5), the telescopic rod (52) is opened again, so that the slider (53) slides in the direction close to the fixed block (51). At this time, the two groups of grabbing racks (54) will not only slide in the direction close to the fixed block (51), but also unfold to each other, so that the two groups of grabbing racks (54) can place the used pipette (10) in the first conveying box (41) through the grabbing groove (55), thereby realizing the placement of the pipette (10).

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