Activating and transferring device for preserved strains

Abstract

The preserved strain activating and transferring device comprises a base, a supporting back plate is upwards arranged on the rear side of the base, two placement sleeves are oppositely arranged at the positions, on the front side of the supporting back plate, of the upper end of the base, a top mounting table is arranged at the upper end of the supporting back plate, and a first linear motor is mounted at the lower end of the top mounting table; a rotor of the first linear motor is downwards connected with a vertically-arranged telescopic motor, a telescopic shaft of the telescopic motor is vertically downwards provided with a vertical connecting strip, a telescopic motor is installed on one side of the vertical connecting strip, and a limiting channel is formed in the lower end of the vertical connecting strip. A telescopic shaft of the telescopic motor is downwards connected with a J-shaped elastic scraping piece penetrating through the limiting channel. A second linear motor is installed in the middle of the supporting back plate, a rotor of the second linear motor is connected with a vertically-arranged vertical sliding rail, and the vertical sliding rail is connected with an electric heating ring in a sliding mode. According to the invention, automatic equipment is adopted to replace manpower for strain transfer, so that the working efficiency can be effectively improved, and the risk of cross contamination is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial preservation, and particularly relates to an activation transfer device for preserved strains. Background Art

[0002] In the research and application fields of microbiology, the preservation and activation transfer of strains are important steps to ensure the long-term preservation of microbial resources and maintain their biological activities. Especially in industrial fermentation, biopharmaceuticals, and basic scientific research, the efficient preservation and accurate activation transfer of strains are crucial for the accuracy of experimental results, the stability of the production process, and the quality of products. To prevent strain degradation and ensure its genetic stability, methods such as cryopreservation or freeze-drying are usually used for long-term preservation of strains.

[0003] When these preserved strains need to be used, they must first be activated, that is, the strains in a dormant state are restored to an actively growing state. This process generally includes taking out the strains from specific preservation conditions and inoculating them onto a suitable culture medium for cultivation. This step not only requires the operator to have professional knowledge and skills, but also requires strict control of environmental conditions to avoid contamination or damage to the vitality of the strains. The traditional method is to manually sample the preserved strains from the original culture test tube in a laboratory environment and then transfer them to the fresh culture medium in a new culture test tube to achieve the resuscitation and amplification of the strains.

[0004] However, in the prior art, the activation transfer process of such preserved strains completely relies on manual operation. Although manual operation can flexibly meet the specific requirements of different strains, it also has obvious limitations: the whole process is cumbersome and complex, time-consuming and laborious, especially inefficient when dealing with a large number of samples; in addition, due to the influence of human factors, the risk of cross-contamination of samples may also increase, affecting the reliability of experimental data. Summary of the Invention

[0005] The purpose of the present invention is to provide an activation transfer device for preserved strains, which uses an automated device to replace manual operation for strain transfer, can effectively improve work efficiency, and reduce the risk of cross-contamination.

[0006] The above technical purpose of the present invention is achieved through the following technical solutions: An activation transfer device for preserved strains includes a base. A support backboard is upwardly provided at the rear of the base. Two placement sleeves with open upper ends are oppositely arranged on the upper end of the base and in front of the support backboard. A top mounting table is provided above the placement sleeves at the upper end of the support backboard. A first linear motor is installed at the lower end of the top mounting table. The first linear motor includes a first magnetic rail installed at the lower end of the top mounting table and arranged along its length direction, and a first mover moving along the first magnetic rail.

[0007] The first mover is connected downward to a vertically arranged telescopic motor. The telescopic shaft of the telescopic motor is vertically downward and is provided with a vertical connecting bar. One side of the vertical connecting bar is provided with a vertically arranged telescopic motor. The lower end of the vertical connecting bar is provided with a flat-shaped limiting channel with openings at both the upper and lower ends. The telescopic shaft of the telescopic motor is connected downward to an elastic scraping piece passing through the limiting channel. After the elastic scraping piece extends downward out of the limiting channel, it is in a J shape.

[0008] A second linear motor is installed in the middle of the support back plate. The second linear motor includes a second magnetic rail horizontally arranged on the support back plate and a second mover moving along the second magnetic rail. The second mover is connected with a vertically arranged vertical slide rail. The vertical slide rail is slidably connected with a lifting slider. A driving motor for driving the lifting slider to slide is installed on the vertical slide rail. The lifting slider is connected with a sterilizing collar that can move to be coaxial with each placement sleeve. An electric heating ring is arranged in the sterilizing collar.

[0009] By adopting the above technical solution, when activating and transferring bacterial strains, first prepare a new test tube. When loading the prepared culture medium into the test tube, place the new test tube into one of the placement sleeves. And the second linear motor and the driving motor drive the electric heating ring to move and sleeve it on the opening of the new test tube. The electric heating ring is powered on to perform high-temperature sterilization on the opening end of the new test tube.

[0010] Take the original test tube that needs to be transferred. After opening the sealing plug, drive the electric heating ring to move and sleeve it on the opening of the original test tube to perform high-temperature sterilization on the opening end of the original test tube. And hold the sealing plug to also perform sterilization through the electric heating ring.

[0011] Subsequently, driven by the first linear motor and the telescopic motor, the lower end of the vertical connecting bar is lifted into the original test tube. When reaching the colony position, the telescopic motor drives the elastic scraping piece to extend out of the limiting channel and bend into a J shape, and insert it into the colony. The telescopic motor shortens to drive the vertical connecting bar to drive the elastic scraping piece to move upward, scraping away a part of the colony. Then drive the vertical connecting bar to drive the elastic scraping piece to move the colony into the new test tube. The telescopic motor shortens to drive the elastic scraping piece to retract into the limiting channel, scraping the colony into the new test tube to complete the transfer.

[0012] Finally, drive the electric heating ring to move and sleeve the lower end of the vertical connecting bar, drive the elastic scraping piece to extend, and use the electric heating ring to fully sterilize the elastic scraping piece to avoid cross-contamination later. At the same time, the original test tube and the new test tube also need to be sterilized at high temperature by the electric heating ring, and the rubber plugs for sealing the original test tube and the new test tube are also sterilized by the electric heating ring before sealing.

[0013] A further setting of the present invention is that the lower end of the telescopic motor is connected to the elastic scraper through a connector, and the connector cannot enter the limiting channel.

[0014] A further setting of the present invention is that a slope scraper head that is downward and inclined toward the elastic scraper is provided on the side where the lower end of the vertical connecting bar bends toward the elastic scraper.

[0015] A further setting of the present invention is that the elastic scraper is made of titanium alloy material.

[0016] A further setting of the present invention is that a vertically arranged chute with a front opening is provided in the vertical slide rail, a vertical threaded shaft is rotatably connected in the vertically arranged chute, the driving motor is installed at one end of the vertical slide rail and its power output shaft is connected to one end of the vertical threaded shaft, and a threaded slide hole that is threadedly connected to the vertical threaded shaft is provided on the lifting slider.

[0017] A further setting of the present invention is that horizontal limiting slide rails are provided at both the upper and lower ends of the vertical slide rail on the front side of the support back plate, and limiting sliders that are slidably connected to the corresponding horizontal limiting slide rails are provided at both ends of the vertical slide rail.

[0018] A further setting of the present invention is that the electric heating ring is an annular infrared heating lamp tube.

[0019] In summary, the present invention has the following beneficial effects:

[0020] The present invention uses automated equipment to replace manual labor for complex and cumbersome transfer work. Each link in the process is automatically sterilized and disinfected, and the colonies are automatically transferred to a new test tube for cultivation, reducing the labor intensity, improving the work efficiency, and during the mechanical operation process, it is not easy to shake, and the taken-out colonies will not be shaken to the ground, and it can transfer the colonies to a new test tube efficiently and accurately, reducing the risk of sample cross-contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 used to show the connection between the elastic scraper and the limiting channel;

[0023] Figure 3 is a partial cross-sectional view used to show the limiting channel;

[0024] Figure 4 used to show the connection between the sterilization collar and the vertical slide rail.

[0025] In the figure: 1. Base; 11. Support backplate; 12. Placing sleeve; 13. Top mounting table; 14. Horizontal limiting slide rail; 2. First linear motor; 21. First magnetic rail; 22. First mover; 3. Telescopic motor; 4. Vertical connecting bar; 41. Limiting channel; 42. Inclined scraping head; 5. Telescopic motor; 51. Connector; 52. Elastic scraping blade; 6. Second linear motor; 61. Second magnetic rail; 62. Second mover; 7. Vertical slide rail; 71. Lifting slider; 72. Vertical chute; 73. Vertical threaded shaft; 74. Driving motor; 75. Threaded slide hole; 76. Limiting slider; 8. Sterilizing collar; 81. Electric heating ring. Detailed implementation mode

[0026] The present invention will be further described in detail below with reference to the accompanying drawings.

[0027] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", "front", "rear", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0028] In addition, terms such as "horizontal" and "vertical" do not mean that the components are required to be absolutely horizontal or hanging, but can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0029] In the description of the present invention, it should also be noted that unless otherwise clearly defined and limited, the terms "set", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] Example, referring to Figures 1-4 , a device for activating and transferring preserved bacterial strains, including a base 1. A support backplate 11 is arranged upward at the rear of the base 1. Two placing sleeves 12 with upper openings are oppositely arranged on the upper end of the base 1 on the front side of the support backplate 11. A top mounting table 13 is arranged above the placing sleeves 12 at the upper end of the support backplate 11. A first linear motor 2 is installed at the lower end of the top mounting table 13. The first linear motor 2 includes a first magnetic rail 21 installed at the lower end of the top mounting table 13 and arranged along its length direction, and a first mover 22 moving along the first magnetic rail 21.

[0031] A first mover 22 is connected downward to a vertically arranged telescopic motor 3. The telescopic shaft of the telescopic motor 3 is vertically downward provided with a vertical connecting bar 4. One side of the vertical connecting bar 4 is installed with a vertically arranged telescopic motor 5. The lower end of the vertical connecting bar 4 is provided with a flat-shaped limiting channel 41 with openings at both upper and lower ends. The telescopic shaft of the telescopic motor 5 is connected downward to a connecting head 51. The connecting head 51 cannot enter the limiting channel 41. The connecting head 51 is connected downward to an elastic scraping blade 52 passing through the limiting channel 41. The elastic scraping blade 52 is made of titanium alloy material, so that it has good elastic recovery force and corrosion resistance. After the elastic scraping blade 52 extends downward out of the limiting channel 41, it is in a J shape, which is convenient for scraping the bacterial strain sample. One side of the lower end of the vertical connecting bar 4 facing the bent elastic scraping blade 52 is provided with an inclined scraping head 42 that is downward and inclined toward the elastic scraping blade 52. Through the inclined scraping head 42, after the elastic scraping blade 52 retracts, the bacterial strain on it can be scraped off.

[0032] A second linear motor 6 is installed in the middle of the support back plate 11. The second linear motor 6 includes a second magnetic rail 61 installed on the support back plate 11 and arranged horizontally, and a second mover 62 moving along the second magnetic rail 61. The second mover 62 is connected with a vertically arranged vertical slide rail 7. The vertical slide rail 7 is slidably connected with a lifting slider 71. A vertically arranged vertical chute 72 with an open front side is arranged in the vertical slide rail 7. A vertical threaded shaft 73 is rotatably connected in the vertical chute 72. The lower end of the vertical slide rail 7 is installed with a driving motor 74. The power output shaft of the driving motor 74 is connected with one end of the vertical threaded shaft 73. The lifting slider 71 is provided with a threaded slide hole 75 threadedly connected with the vertical threaded shaft 73. On the front side of the support back plate 11, horizontal limiting slide rails 14 are arranged at both upper and lower ends of the vertical slide rail 7. Both ends of the vertical slide rail 7 are provided with a limiting slider 76 slidably connected with the corresponding horizontal limiting slide rail 14. The lifting slider 71 is connected with a sterilization collar 8 that can move to be coaxial with each placement sleeve 12, so that the sterilization collar 8 can cover the culture test tube. An electric heating ring 81 is arranged in the sterilization collar 8. The electric heating ring 81 is an annular infrared heating lamp tube, which can quickly generate high temperature for sterilization and disinfection.

[0033] Usage method: When activating and transferring the bacterial strain, first prepare a new test tube. When loading the prepared culture medium into the test tube, put the new test tube into one of the placement sleeves 12, and the second linear motor 6 and the driving motor 74 drive the electric heating ring 81 to move and cover the opening of the new test tube. The electric heating ring 81 is powered on to perform high-temperature sterilization and disinfection on the opening end of the new test tube;

[0034] Take the original test tube that needs to be transferred. After opening the sealing plug, drive the electric heating ring 81 to move and sleeved on the opening of the original test tube to perform high-temperature sterilization on the opening end of the original test tube, and the hand-held sealing plug is also sterilized through the electric heating ring 81;

[0035] Subsequently, driven by the first linear motor 2 and the telescopic motor 3, the lower end of the vertical connecting bar 4 is lifted into the original test tube. After reaching the colony position, the telescopic motor 5 drives the elastic scraping blade 52 to extend from the limiting channel 41 and bend into a J shape, and insert it into the colony. The telescopic motor 3 shortens to drive the vertical connecting bar 4 to drive the elastic scraping blade 52 to move upward, scraping away a part of the colony. Then drive the vertical connecting bar 4 to drive the elastic scraping blade 52 to move the colony into the new test tube. The telescopic motor 5 shortens to drive the elastic scraping blade 52 to retract into the limiting channel 41, and the colony is scraped into the new test tube by the inclined scraping head 42 to complete the transfer;

[0036] Finally, drive the electric heating ring 81 to move and sleeve the lower end of the vertical connecting bar 4, drive the elastic scraping blade 52 to extend, and use the electric heating ring 81 to fully sterilize the elastic scraping blade 52 to avoid subsequent cross-contamination. At the same time, the original test tube and the new test tube are also sterilized at high temperature by the electric heating ring 81, and the rubber plugs for sealing the original test tube and the new test tube are also sterilized by the electric heating ring 81 before sealing.

[0037] This specific embodiment is only an explanation of the present invention, and it is not a limitation of the present invention. Those skilled in the art can make modifications to this embodiment without creative contributions according to needs after reading this specification, but as long as it is within the scope of the claims of the present invention, it is protected by the patent law.

Claims

1. A preserved bacterial strain activation transfer device, comprising a base (1), characterized in that: A supporting back plate (11) is arranged upward at the rear side of the base (1); two placement sleeves (12) with upper ends opened are arranged at the upper end of the base (1) opposite to the front side of the supporting back plate (11); a top mounting platform (13) is arranged above the placement sleeves (12) at the upper end of the supporting back plate (11); a first linear motor (2) is installed at the lower end of the top mounting platform (13); the first linear motor (2) comprises a first magnetic rail (21) installed at the lower end of the top mounting platform (13) and arranged along its length direction, and a first mover (22) moving along the first magnetic rail (21); The first mover (22) is connected downwardly to a telescopic motor (3) arranged vertically, the telescopic shaft of the telescopic motor (3) is provided with a vertical connecting bar (4) vertically downwardly, a telescopic motor (5) arranged vertically is installed on one side of the vertical connecting bar (4), a flat limiting channel (41) with openings at both ends is provided at the lower end of the vertical connecting bar (4), the telescopic shaft of the telescopic motor (5) is connected downwardly to an elastic scraper (52) passing through the limiting channel (41), and the elastic scraper (52) extends downwardly out of the limiting channel (41) to form a J shape; A second linear motor (6) is installed in the middle of the support back plate (11), and the second linear motor (6) comprises a second magnetic rail (61) installed on the support back plate (11) and arranged horizontally, and a second mover (62) moving along the second magnetic rail (61), the second mover (62) is connected to a vertical slide rail (7) arranged vertically, the vertical slide rail (7) is slidably connected to a lifting slider (71), a driving motor (74) for driving the lifting slider (71) to slide is installed on the vertical slide rail (7), the lifting slider (71) is connected to a sterilization ring (8) that can be moved to be coaxial with each placement sleeve (12), and an electric heating ring (81) is arranged inside the sterilization ring (8).

2. The activation transfer device for preserved bacterial strains according to claim 1, characterized in that: The lower end of the telescopic motor (5) is connected to the elastic scraper (52) via a connector (51), and the connector (51) cannot enter the limiting channel (41).

3. The activation transfer device for preserved bacterial strains according to claim 1, characterized in that: A bevel scraper head (42) inclined downward and toward the elastic scraper (52) is provided on the side of the lower end of the vertical connecting strip (4) that is bent toward the elastic scraper (52).

4. The activation transfer device for preserved bacterial strains according to claim 1, characterized in that: The elastic scraper (52) is made of titanium alloy.

5. The activation transfer device for preserved bacterial strains according to claim 1, characterized in that: The vertical slide rail (7) is provided with a vertical slide groove (72) with a front opening, a vertical threaded shaft (73) is rotatably connected in the vertical slide groove (72), the driving motor (74) is installed at one end of the vertical slide rail (7) and its power output shaft is connected to one end of the vertical threaded shaft (73), and the lifting slider (71) is provided with a threaded slide hole (75) threadedly connected to the vertical threaded shaft (73).

6. The activation transfer device for preserved bacterial strains according to claim 1, characterized in that: The front side of the supporting back plate (11) is provided with horizontal limit rails (14) at both upper and lower ends of the vertical rails (7), and both ends of the vertical rails (7) are provided with limit sliders (76) slidably connected to the corresponding horizontal limit rails (14).

7. The activation transfer device for preserved bacterial strains according to claim 1, characterized in that: The electric heating ring (81) is a ring-shaped infrared heating lamp tube.

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