Device for regulating and controlling macrophage culture

Abstract

The invention relates to the technical field of cell culture, in particular to a regulation and control macrophage culture device which comprises a case and a base, the base is fixedly mounted at the front end of the case, a rotating assembly is arranged in the base, a culture bottle is placed in the rotating assembly, the lower end of the culture bottle is connected with a bottom plate, and the bottom plate is fixedly connected with the base. A lifting mechanism is arranged on the upper side of the culture bottle, and a supply mechanism is arranged in the case. Through the arrangement of the rotating assembly, a culture medium containing cytokines or other substances are automatically supplemented into the culture bottle through the storage tank, and the servo motor is started to drive the tray to rotate the culture bottle, so that the culture medium or stimulating factors are distributed more uniformly through centrifugal force; and the servo motor has a forward and reverse rotation mode and different rotation gears, so that the mixing effect is further improved, insufficient local mixing caused by unidirectional rotation is avoided, and the consistency of the culture environment is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and particularly to a macrophage culture device for regulation. Background Art

[0002] Macrophages are a type of white blood cell present in tissues, differentiated from monocytes, which in turn originate from precursor cells in the bone marrow. Macrophages and monocytes both belong to phagocytic cells and participate in the processes of non-specific and specific immune defenses in vertebrates. Their main functions include phagocytosing cell debris and pathogens in the form of fixed or free cells, and at the same time being able to activate lymphocytes or other immune cells to produce an immune response against pathogens. As a type of immune cell, macrophages have multiple functions and are key objects of research in cell phagocytosis, cellular immunity, and molecular immunology. However, macrophages belong to a non-replicating cell population, are easily induced to differentiate by external conditions during in vitro culture, and can only survive for 2 - 3 weeks even under suitable conditions, making it difficult to survive for a long time. This cell culture device provides a suitable growth environment for macrophages, avoiding external condition stimuli while maintaining an ideal state of macrophages. Given that macrophages are very different from other cells in terms of morphological structure, culture characteristics, and the required microenvironment, traditional culture devices cannot provide suitable culture conditions for macrophages, such as precise mechanical support, appropriate nutrient supply modes, and simulation of the in vivo immune microenvironment, making it difficult to meet the needs of large-scale macrophage culture, especially not meeting the requirements of macrophage-based tissue engineering. Therefore, this urgently promotes the research and development of a culture device specifically for macrophages.

[0003] Currently, the difficulty in culturing macrophages in vitro lies in that existing macrophages need to continuously supplement some special cytokines to maintain their characteristics, including normal and activated states, etc. However, after the addition of the cytokine-containing medium, due to the fixed position during the addition of the medium, it is easy for the cytokine-containing medium to show uneven distribution after being added to the culture flask, resulting in differences in the contact of macrophages at different positions with cytokines, and thus easily causing growth differences in macrophages. In addition, cytokines need to be supplemented at a high frequency, but the current manual experimental operations increase the probability of cell contamination. For this reason, we propose a culture device for regulating macrophages to solve the above problems. Summary of the Invention

[0004] The present invention is committed to providing a novel macrophage culture device for regulation, focusing on two key challenges existing in the background art: one is the uneven distribution situation generated after adding cytokines to the culture device, and the other is the contamination risk caused by high-frequency manual operations.

[0005] To achieve the above object, the present invention provides the following technical solution: A macrophage culture regulation device, comprising a chassis and a base. The front end of the chassis is fixedly installed with a base. A rotation assembly is arranged inside the base. A culture bottle is placed inside the rotation assembly. The lower end of the culture bottle is connected to a bottom plate. A lifting mechanism is arranged above the culture bottle. A supply mechanism is arranged inside the chassis;

[0006] The lifting mechanism includes a second fixing groove, an electric telescopic rod, a connecting plate, a first connecting rod, a bottle cap and a guide rod. A second fixing groove is opened inside the chassis. The electric telescopic rod is installed inside the second fixing groove. The upper end of the electric telescopic rod is fixedly connected to the connecting plate. The bottom of the connecting plate is rotatably connected to the first connecting rod. The lower end of the first connecting rod is fixedly connected to the bottle cap. The bottle cap is located directly above the culture bottle;

[0007] The supply mechanism includes a storage tank, a filtering assembly, a delivery pump and a first connecting pipe. Two groups of storage tanks are fixedly installed above the connecting plate inside the chassis through four columns. A filtering assembly is installed on the upper side inside the storage tank. Delivery pumps are respectively installed at the bottoms of the two groups of storage tanks inside the four columns. A first connecting pipe is arranged inside the delivery pump. One end of the first connecting pipe is connected to the storage tank. The other end of the first connecting pipe is connected to a second connecting pipe. The lower end of the second connecting pipe is connected to the bottle cap.

[0008] Preferably, the rotation assembly includes a first fixing groove, a servo motor, a tray and a positioning groove. A first fixing groove is opened inside the base. The bottom of the base is fixedly installed with a servo motor. The upper output end of the servo motor is fixedly connected to the tray. A positioning groove is opened at the center position of the tray. The diameter of the positioning groove is adapted to the bottom plate.

[0009] Preferably, two groups of guide rods are symmetrically installed around the electric telescopic rod inside the second fixing groove. Two holes adapted to the guide rods are opened inside the connecting plate. The inner diameter of the first connecting rod is adapted to the diameter of the culture bottle.

[0010] Preferably, a fixing assembly is arranged inside the tray. The fixing assembly includes a first movable groove, a second connecting rod, an insertion block, a pull rod, a first spring and a card slot. Two groups of first movable grooves are symmetrically opened inside the tray. The second connecting rods are respectively movably connected inside the two groups of first movable grooves. The inner sides of the second connecting rods are fixedly connected to the insertion blocks. The outer sides of the second connecting rods are fixedly connected to the pull rods. A first spring is arranged inside the first movable groove. The two ends of the first spring respectively abut against the second connecting rod and the inner wall of the first movable groove. Two groups of card slots are symmetrically opened inside the bottom plate. The diameter of the card slot is adapted to the insertion block. Two groups of third fixing grooves are symmetrically opened inside the base.

[0011] Preferably, a guiding assembly is provided on the outer side of the first connecting rod. The guiding assembly includes an external thread, a movable ring, a third connecting rod, a protective assembly, and a connecting ring. An external thread is provided on the outer surface of the first connecting rod. A movable ring is movably connected to the outer side of the first connecting rod. Two groups of third connecting rods are fixedly connected to the left and right ends of the movable ring. A connecting ring is provided on the outer side of the third connecting rod. The connecting ring is arranged at an angle of 45 degrees. Two groups of the second connecting pipes pass through the connecting ring and are connected to the bottle cap. The inner wall of the connecting ring is smooth. A protective assembly is provided between the third connecting rod and the connecting ring.

[0012] Preferably, the protective assembly includes a second movable groove, a slider, a fourth connecting rod, a gasket, and a second spring. A second movable groove is provided in the third connecting rod. A slider is movably connected in the second movable groove. A fourth connecting rod is fixedly connected to the outer side of the slider. The outer side of the fourth connecting rod is fixedly connected to the connecting ring. A second spring is provided in the second movable groove.

[0013] Preferably, the slider is spherical. A gasket is provided between the second spring and the slider. The diameter of the gasket is adapted to the inner diameter of the second movable groove.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0015] 1. Through the setting of the rotating assembly, the present invention automatically supplements the culture medium or other substances containing cytokines from the storage tank into the culture bottle. Before entering the culture bottle, it first passes through the filtering assembly provided at the replenishment port of the storage tank to prevent impurities and pollutants from entering the culture system, especially microorganisms or undissolved particulate matter that may be introduced during the replenishment process. Then, the servo motor is started to drive the tray to rotate the culture bottle, so that the culture medium containing cytokines is more evenly distributed by centrifugal force. Moreover, the servo motor has forward and reverse rotation modes and different rotation gears, further improving the mixing effect and avoiding uneven local mixing caused by single-direction rotation, ensuring the consistency of the culture environment.

[0016] 2. Through the setting of the fixing assembly, the culture bottle is inserted into the positioning groove through the bottom plate, and the insertion block in the positioning groove fixes the bottom plate. The electric telescopic rod drives the connecting plate and the bottle cap to move up and down, which is convenient for fixing and disassembling the culture bottle. The detachable structural design facilitates separating the culture bottle from the tray and the bottle cap. Such a separation mode is beneficial for taking out the culture bottle separately for observation under a microscope to conveniently monitor the cell growth state in real time on the one hand; on the other hand, it is convenient for regularly cleaning the cells and replacing the fresh culture medium, and collecting the cells at the end of the culture.

[0017] 3. With the provision of the guiding component, when the culture bottle rotates, it drives the first connecting rod to rotate, causing the outer movable ring to rotate through the external thread on the surface of the first connecting rod. Under the guidance of the two connecting rings, the second connecting pipe is guided to prevent entanglement between the two second connecting pipes. Moreover, as the culture bottle continues to rotate, the connecting ring drives the second connecting pipe to move into the second movable groove through the slider, preventing the second connecting pipe from being damaged due to excessive pulling force. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following-described drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0019] Figure 1 is a front view structural schematic diagram of the present invention;

[0020] Figure 2 is a structural schematic diagram of the lifting mechanism and the supply mechanism of the present invention;

[0021] Figure 3 is a structural schematic diagram of the rotating component of the present invention;

[0022] Figure 4 is of the present invention Figure 1 is a schematic cross-sectional view of the structure of the base in the present invention;

[0023] Figure 5 is a structural schematic diagram of the guiding component of the present invention;

[0024] Figure 6 is of the present invention Figure 4 is a partially enlarged schematic diagram of A in the present invention;

[0025] Figure 7 is of the present invention Figure 5 is a partially enlarged schematic diagram of B in the present invention.

[0026] In the figure: 1. Chassis; 2. Base; 3. Rotating assembly; 31. First fixing groove; 32. Servo motor; 33. Tray; 34. Positioning groove; 4. Culture bottle; 5. Lifting mechanism; 51. Second fixing groove; 52. Electric telescopic rod; 53. Connecting plate; 54. First connecting rod; 55. Bottle cap; 56. Guide rod; 6. Supply mechanism; 61. Storage tank; 62. Filter assembly; 63. Delivery pump; 64. First connecting pipe; 65. Second connecting pipe; 7. Bottom plate; 8. Fixing assembly; 81. First movable groove; 82. Second connecting rod; 83. Insert block; 84. Pull rod; 85. First spring; 86. Card slot; 9. Third fixing groove; 10. Guide assembly; 101. External thread; 102. Movable ring; 103. Third connecting rod; 104. Protection assembly; 1041. Second movable groove; 1042. Slide block; 1043. Fourth connecting rod; 1044. Gasket; 1045. Second spring; 105. Connecting ring. Detailed implementation mode

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] Please refer to Figures 1-7 , an embodiment provided by the present invention: A macrophage culture device for regulation, including a chassis 1 and a base 2. A base 2 is fixedly installed at the front end of the chassis 1. A rotating assembly 3 is arranged inside the base 2. A culture bottle 4 is placed inside the rotating assembly 3. The lower end of the culture bottle 4 is connected to a bottom plate 7. A lifting mechanism 5 is arranged above the culture bottle 4. A supply mechanism 6 is arranged inside the chassis 1; the culture bottle 4 is made of transparent glass material, which is convenient to be removed and observed under a microscope.

[0029] The lifting mechanism 5 includes a second fixing groove 51, an electric telescopic rod 52, a connecting plate 53, a first connecting rod 54, a bottle cap 55 and a guide rod 56. A second fixing groove 51 is opened inside the chassis 1. An electric telescopic rod 52 is installed inside the second fixing groove 51. The upper end of the electric telescopic rod 52 is fixedly connected to a connecting plate 53. The bottom of the connecting plate 53 is rotatably connected to a first connecting rod 54. The lower end of the first connecting rod 54 is fixedly connected to a bottle cap 55. The bottle cap 55 is located directly above the culture bottle 4. A sealing ring is arranged inside the bottle cap 55; by starting the electric telescopic rod 52, the connecting plate 53 is driven to move downward inside the second fixing groove 51, and the bottle cap 55 at the bottom of the first connecting rod 54 is covered on the culture bottle 4. The bottle cap 55 is also made of transparent plastic material.

[0030] The supply mechanism 6 includes a storage tank 61, a filtration component 62, a delivery pump 63, and a first connecting pipe 64. On the upper side of the connecting plate 53, two groups of storage tanks 61 are fixedly installed inside the chassis 1 through four columns. A filtration component 62 is installed on the upper side inside the storage tank 61. Delivery pumps 63 are respectively installed at the bottoms of the two groups of storage tanks 61 inside the four columns. A first connecting pipe 64 is arranged inside the delivery pump 63. One end of the first connecting pipe 64 is connected to the storage tank 61, and the other end of the first connecting pipe 64 passes through the inside of the connecting plate 53 and is connected to a second connecting pipe 65. The lower end of the second connecting pipe 65 is connected to the bottle cap 55. The culture medium is supplemented internally through the supplement port at the storage tank 61. The culture medium enters the storage tank 61 through the filtration component 62, preventing impurities from entering the culture system and avoiding introducing microorganisms or undissolved particulate matter during the supplementation process. Start the delivery pump 63, and the culture medium in the storage tank 61 flows into the second connecting pipe 65 through the first connecting pipe 64, and then enters the inside of the culture bottle 4 through the second connecting pipe 65.

[0031] Through the settings of the rotation component 3, the lifting mechanism 5, and the supply mechanism 6, this device solves the problems that uneven distribution of cytokines will cause significant differences in the amount of cytokines obtained by macrophages in different regions of the culture device, seriously interfering with the normal growth rhythm of macrophages; the pollution risk brought by manual high-frequency operation may cause the culture system to be invaded by exogenous microorganisms, destroying the cell growth environment, and ultimately resulting in uneven growth conditions of macrophages and making it difficult to achieve a stable culture goal. The culture device designed by the present invention can effectively solve the above problems of uneven distribution and pollution risk through innovative structural and functional designs, strongly guarantee the accuracy and stability of the macrophage culture process, and greatly promote the further development of macrophage-related research and applications.

[0032] Further, the rotation component 3 includes a first fixing groove 31, a servo motor 32, a tray 33, and a positioning groove 34. The servo motor 32: The external dimensions are generally between 60 - 120 mm, and the length is between 70 - 120 mm. A first fixing groove 31 is formed inside the base 2. A servo motor 32 is fixedly installed at the bottom of the base 2. A control switch is arranged on the front side of the base 2. The servo motor 32 is electrically connected to the control switch. The three control switches respectively correspond to different gears of the servo motor 32. Adjust the appropriate rotation speed according to the type of culture and experimental requirements. The upper output end of the servo motor 32 is fixedly connected to a tray 33. A positioning groove 34 is formed at the central position of the tray 33. The diameter of the positioning groove 34 is adapted to the bottom plate 7. As Figure 3As shown, this structure is used to start the servo motor 32 by controlling the switch. The servo motor 32 drives the tray 33, causing the culture bottle 4 in the positioning groove 34 to rotate forward and backward, further improving the mixing effect, avoiding local insufficient mixing caused by one-way rotation, and at the same time preventing the second connecting pipe 65 from being pulled due to continuous one-way rotation, affecting the service life of the second connecting pipe 65.

[0033] Furthermore, two groups of guide rods 56 are symmetrically installed in the second fixing groove 51 with the electric telescopic rod 52 as the center. Two holes adapted to the guide rods 56 are opened in the connecting plate 53. The inner diameter of the first connecting rod 54 is adapted to the diameter of the culture bottle 4. As Figure 2 shown, this structure is used to improve the stability of the device by driving the connecting plate 53 to move along the guide rods 56 when the electric telescopic rod 52 is started through the arrangement of the guide rods 56.

[0034] Furthermore, a fixing component 8 is arranged in the tray 33. The fixing component 8 includes a first movable groove 81, a second connecting rod 82, an insertion block 83, a pull rod 84, a first spring 85, and a clamping groove 86. Two groups of first movable grooves 81 are symmetrically opened in the tray 33. Two second connecting rods 82 are respectively movably connected in the two groups of first movable grooves 81. An insertion block 83 is fixedly connected to the inner side of the second connecting rod 82. The upper surface of the insertion block 83 is arc-shaped. A pull rod 84 is fixedly connected to the outer side of the second connecting rod 82. A first spring 85 is arranged in the first movable groove 81. The two ends of the first spring 85 respectively abut against the second connecting rod 82 and the inner wall of the first movable groove 81. Two groups of clamping grooves 86 are symmetrically opened in the interior of the bottom plate 7. The diameter of the clamping groove 86 is adapted to the insertion block 83. Two groups of third fixing grooves 9 are symmetrically opened in the base 2. As Figure 6 shown, this structure is used to fix the culture bottle 4 through the arrangement of the fixing component 8. When fixing the culture bottle 4, the bottom plate 7 of the culture bottle 4 is inserted into the positioning groove 34. When inserting the bottom plate 7, due to the downward force, the insertion blocks 83 on both sides are pushed to drive the second connecting rods 82 to move into the first movable grooves 81, and at the same time, the first spring 85 is compressed, causing the first spring 85 to deform. After the bottom plate 7 is completely inserted into the positioning groove 34, under the restoring action of the first spring 85, the insertion block 83 is inserted into the clamping groove 86 to limit the bottom plate 7, thereby fixing the culture bottle 4. When disassembling, only need to pull the pull rod 84 to move towards the third fixing groove 9, so that the insertion block 83 is disengaged from the clamping groove 86, cancel the locking of the bottom plate 7, and the culture bottle 4 can be taken out.

[0035] Further, a guiding component 10 is arranged on the outer side of the first connecting rod 54. The guiding component 10 includes an external thread 101, a movable ring 102, a third connecting rod 103, a protection component 104 and a connecting ring 105. An external thread 101 is provided on the outer surface of the first connecting rod 54. A movable ring 102 is movably connected to the outer side of the first connecting rod 54. Two groups of third connecting rods 103 are fixedly connected to the left and right ends of the movable ring 102. A connecting ring 105 is arranged on the outer side of the third connecting rod 103. The connecting ring 105 is arranged at an angle of forty-five degrees inclined in the rotation direction, which can better guide the second connecting pipe 65. Two groups of second connecting pipes 65 pass through the connecting ring 105 and are connected to the bottle cap 55. And the inner wall of the connecting ring 105 is smooth, so that the second connecting pipe 65 can slide smoothly in the connecting ring 105. The upper and lower openings of the connecting ring 105 are rounded to avoid abrasion of the second connecting pipe 65. A protection component 104 is arranged between the third connecting rod 103 and the connecting ring 105. As Figure 5 shown, this structure is used to drive the bottle cap 55 to rotate by the culture bottle 4 after the bottle cap 55 and the culture bottle 4 are closed. The movable ring 102 on the surface of the first connecting rod 54 is rotated through the external thread 101. While rotating, the two groups of connecting rings 105 on both sides guide the second connecting pipe 65 to avoid knotting or winding during rotation.

[0036] Further, the protection component 104 includes a second movable groove 1041, a slider 1042, a fourth connecting rod 1043, a gasket 1044 and a second spring 1045. A second movable groove 1041 is opened in the third connecting rod 103. A slider 1042 is movably connected in the second movable groove 1041. A fourth connecting rod 1043 is fixedly connected to the outer side of the slider 1042. The outer side of the fourth connecting rod 1043 is fixedly connected to the connecting ring 105. A second spring 1045 is arranged in the second movable groove 1041. As Figure 7 shown, this structure is used to continuously rotate the bottle cap 55, so that the second connecting pipe 65 drives the slider 1042 to move into the second movable groove 1041 through the connecting ring 105 and the fourth connecting rod 1043, and squeezes the second spring 1045 to shorten the distance between the two groups of second connecting pipes 65, avoiding excessive pulling force. When flipping, the second connecting pipe 65 returns to its original position under the action of the connecting ring 105 and the second spring 1045.

[0037] Further, the slider 1042 is spherical. A gasket 1044 is arranged between the second spring 1045 and the slider 1042. The diameter of the gasket 1044 is adapted to the inner diameter of the second movable groove 1041. As Figure 7As shown, this structure is used to utilize the frictional force between the spherical slider 1042 and the second movable groove 1041, and can absorb forces in all directions, enabling the fourth connecting rod 1043 to still slide within the second movable groove 1041 even when not vertically stressed, avoiding jamming. While the slider 1042 slides, it pushes the gasket 1044 to squeeze the second spring 1045, making the force on the second spring 1045 uniform.

[0038] Working principle: When in use, directly place the device in an incubator for use. As Figure 1 and Figure 6 shown, first fix the culture bottle 4 through the bottom plate 7. Insert the bottom plate 7 at the bottom of the culture bottle 4 into the positioning groove 34. When inserting the bottom plate 7, with a downward force, it pushes the insertion blocks 83 on both sides to drive the second connecting rod 82 to move into the first movable groove 81, and at the same time squeeze the first spring 85, causing the first spring 85 to deform. After the bottom plate 7 is completely inserted into the positioning groove 34, under the restoring force of the first spring 85, the insertion blocks 83 are inserted into the card slots 86 to limit the bottom plate 7, thereby fixing the culture bottle 4. As Figure 2 shown, then start the electric telescopic rod 52. The electric telescopic rod 52 drives the connecting plate 53 to move downward along the guide rod 56 to close the bottle cap 55 at the bottom of the first connecting rod 54 with the culture bottle 4. When inputting the culture medium or other substances, start the delivery pump 63. Through the first connecting pipe 64 in the delivery pump 63, the culture medium filtered by the filtering component 62 in the storage tank 61 is introduced into the second connecting pipe 65 and flows into the culture bottle 4 through the second connecting pipe 65. As Figure 3 shown, start the servo motor 32 through the control switch. The servo motor 32 drives the tray 33 to rotate the culture bottle 4 in the positioning groove 34. As Figure 5 and Figure 7 shown, since the top of the first connecting rod 54 is rotatably connected to the connecting plate 53, the culture bottle 4 drives the bottle cap 55 to rotate. When the first connecting rod 54 rotates, the movable ring 102 rotates through the external thread 101 on its surface. While rotating, it guides the second connecting pipe 65 through the two sets of connecting rings 105 on both sides to avoid knotting or winding during rotation. As the bottle cap 55 continues to rotate, the second connecting pipe 65 drives the slider 1042 to move into the second movable groove 1041 through the connecting ring 105 and the fourth connecting rod 1043, pushing the gasket 1044 to squeeze the second spring 1045, shortening the distance between the two second connecting pipes 65 and avoiding excessive pulling force. During flipping, the second connecting pipe 65 returns to its original position under the action of the connecting ring 105 and the second spring 1045. When it is necessary to detect the inside of the culture bottle 4, as Figure 2 shown, first start the electric telescopic rod 52 to drive the connecting plate 53 to separate the bottle cap 55 from the culture bottle 4. As Figure 4and Figure 6 As shown in Figure 6 , when disassembling the culture bottle 4, only need to pull the pull rod 84 to move towards the third fixing groove 9, so that the insertion block 83 disengages from the clamping groove 86, cancel the locking of the bottom plate 7, and then the culture bottle 4 can be taken out. The above is the whole working principle of the present invention.

[0039] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claimed claim.

Claims

1. A macrophage culture device, comprising a housing (1) and a base (2), characterized in that: A base (2) is fixedly mounted at the front end of the chassis (1), a rotating assembly (3) is arranged inside the base (2), a culture bottle (4) is placed inside the rotating assembly (3), a bottom plate (7) is connected to the lower end of the culture bottle (4), a lifting mechanism (5) is arranged on the upper side of the culture bottle (4), and a supply mechanism (6) is arranged inside the chassis (1); The lifting mechanism (5) comprises a second fixed groove (51), an electric telescopic rod (52), a connecting plate (53), a first connecting rod (54), a bottle cap (55) and a guide rod (56); the chassis (1) is provided with a second fixed groove (51); the electric telescopic rod (52) is installed in the second fixed groove (51); the upper end of the electric telescopic rod (52) is fixedly connected to the connecting plate (53); the bottom of the connecting plate (53) is rotatably connected to the first connecting rod (54); the lower end of the first connecting rod (54) is fixedly connected to the bottle cap (55); the bottle cap (55) is located directly above the culture bottle (4); The replenishing mechanism (6) comprises a storage tank (61), a filter assembly (62), a delivery pump (63) and a first connecting pipe (64); the upper side of the connecting plate (53) is located in the chassis (1) and is fixedly mounted with two groups of storage tanks (61) via four columns; the upper side of the storage tanks (61) is mounted with a filter assembly (62); the bottoms of the two groups of storage tanks (61) are located in the four columns and are respectively mounted with delivery pumps (63); a first connecting pipe (64) is arranged in the delivery pump (63); one end of the first connecting pipe (64) is connected to the storage tank (61); the other end of the first connecting pipe (64) is connected to the second connecting pipe (65); the lower end of the second connecting pipe (65) is connected to the bottle cap (55).

2. The macrophage culture device according to claim 1, characterized in that: The rotating assembly (3) comprises a first fixing groove (31), a servo motor (32), a tray (33) and a positioning groove (34); the first fixing groove (31) is provided in the base (2); the servo motor (32) is fixedly mounted at the bottom of the base (2); the tray (33) is fixedly connected to the upper output end of the servo motor (32); a positioning groove (34) is provided at the center of the tray (33); and the diameter of the positioning groove (34) is adapted to the bottom plate (7).

3. The macrophage culture device according to claim 1, characterized in that: Two groups of guide rods (56) are symmetrically installed in the second fixing groove (51) with the electric telescopic rod (52) as the center, and two groups of holes matched with the guide rods (56) are opened in the connecting plate (53). The inner diameter of the first connecting rod (54) is matched with the diameter of the culture bottle (4).

4. The macrophage culture device according to claim 2, characterized in that: The tray (33) is provided with a fixing assembly (8), the fixing assembly (8) comprising a first movable groove (81), a second connecting rod (82), an insert block (83), a pull rod (84), a first spring (85) and a slot (86), two groups of first movable grooves (81) are symmetrically provided in the tray (33), the two groups of first movable grooves (81) are respectively movably connected to the second connecting rod (82), and the inner side of the second connecting rod (82) is fixedly connected to the insert block (83). ), a pull rod (84) is fixedly connected to the outer side of the second connecting rod (82), a first spring (85) is arranged in the first movable groove (81), two ends of the first spring (85) are respectively against the inner walls of the second connecting rod (82) and the first movable groove (81), two groups of slots (86) are symmetrically opened inside the bottom plate (7), the diameter of the slots (86) is adapted to the plug block (83), and two groups of third fixed slots (9) are symmetrically opened inside the base (2).

5. The macrophage culture device according to claim 1, characterized in that: A guide assembly (10) is arranged on the outer side of the first connecting rod (54), and the guide assembly (10) comprises an external thread (101), a movable ring (102), a third connecting rod (103), a protective assembly (104) and a connecting ring (105). The outer surface of the first connecting rod (54) is provided with an external thread (101), and the outer side of the first connecting rod (54) is movably connected with a movable ring (102). Two groups of third connecting rods (103) are fixedly connected to the left and right ends of the movable ring (102). A connecting ring (105) is arranged on the outer side of the third connecting rod (103), and the connecting ring (105) is inclined at an angle of forty-five degrees. Two groups of the second connecting pipes (65) pass through the connecting ring (105) and are connected to the bottle cap (55), and the inner wall of the connecting ring (105) is smooth. A protective assembly (104) is arranged between the third connecting rod (103) and the connecting ring (105).

6. The macrophage culture device according to claim 5, characterized in that: The protection component (104) comprises a second movable groove (1041), a slider (1042), a fourth connecting rod (1043), a gasket (1044) and a second spring (1045); the second movable groove (1041) is provided in the third connecting rod (103); the slider (1042) is movably connected in the second movable groove (1041); the outer side of the slider (1042) is fixedly connected to the fourth connecting rod (1043); the outer side of the fourth connecting rod (1043) is fixedly connected to the connecting ring (105); and the second spring (1045) is provided in the second movable groove (1041).

7. The macrophage culture device according to claim 6, characterized in that: The slider (1042) is spherical, and a gasket (1044) is provided between the second spring (1045) and the slider (1042), and the diameter of the gasket (1044) is matched with the inner diameter of the second movable groove (1041).

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