In-vivo patch clamp perfusion device

Abstract

The invention belongs to the technical field of patch clamp perfusion dosing, and particularly relates to an in-vivo patch clamp perfusion device which comprises a frame body, an adjusting piece, a liquid medicine tube and a combiner box, the adjusting piece is arranged on a hanging frame of the frame body, a clamping plate for clamping the liquid medicine tube is arranged on a shell of the adjusting piece, the clamping plate is rotationally arranged on a movable seat arranged on a screw rod in a sliding mode, and the movable seat is arranged on the frame body. The moving seat abuts against the supporting spring. The adjustable frame body and the adjusting part are used for installing and fixing the liquid medicine pipe for temporarily storing the liquid medicine, in the using process, the height of the adjusting part and the height of the clamped liquid medicine pipe are directly adjusted on the frame body, meanwhile, the height of the liquid medicine pipe can be finely adjusted on the adjusting part, and therefore the liquid medicine pipe can be conveniently adjusted. The height of the liquid medicine tube is adjusted in real time in the liquid medicine discharging process, so that it is guaranteed that the liquid level in the liquid medicine tube is always located at the proper position, it is avoided that perfusion is affected along with descending of the liquid level of liquid medicine discharging, stable and smooth perfusion is guaranteed, and the perfusion quality and efficiency are improved.

Description

Technical Field

[0001] The invention relates to the technical field of patch clamp perfusion drug delivery, in particular to an in vivo patch clamp perfusion device. Background Art

[0002] Patch clamp, also known as single-channel current recording technology, uses a special glass micropipette to adsorb on the cell surface to form a 10-100 seal, also known as giant resistance sealing. The isolated small patch has an area of ​​μm and only a few ion channels. The patch is then voltage clamped to measure the current of pA (10 to the negative 12th power ampere) generated by the opening of a single ion channel. This channel opening is a random process.

[0003] In the prior art, in patch clamp experiments, drugs need to be provided through a perfusion drug delivery device to facilitate the experiment. The existing patch clamp drug delivery device mainly uses gravity perfusion during the drug delivery process. However, the existing gravity perfusion will affect the perfusion flow rate as the drug solution continues to decrease, which will have a certain impact on the experimental process and is not convenient to use. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies of the prior art, the present invention provides an in vivo patch clamp perfusion device, which solves the problem that during the gravity perfusion process, as the drug solution continues to decrease, the perfusion flow rate is affected, which will have a certain impact on the experimental process and is inconvenient to use.

[0006] (II) Technical solution

[0007] In order to achieve the above-mentioned purpose, the present invention specifically adopts the following technical solutions:

[0008] An in vivo patch clamp perfusion device comprises a frame, an adjusting member, a drug liquid tube and a junction box, wherein the adjusting member is arranged on a hanging frame of the frame, a clamping plate for clamping the drug liquid tube is arranged on a shell of the adjusting member, the clamping plate is rotatably arranged on a movable seat slidably arranged on a screw rod, the movable seat abuts against a supporting spring, and the drug liquid tube is connected to the junction box through a liquid outlet pipe, the junction box is arranged on a connecting frame of the frame, and a drug administration tube is arranged on the junction box.

[0009] Furthermore, a hook is fixedly connected to the outer wall of the rear part of the shell, the hook is matched with the bracket, and the upper end of the screw is fixedly connected to the shell, the support spring is sleeved on the screw, the lower end of the support spring abuts against the adjustment block threadedly connected to the screw, and the movable seat is slidably arranged in the inner cavity of the shell, and a through hole for sliding and inserting the screw is opened on the movable seat;

[0010] The moving seat slides against the inner wall of the housing. A rubber inner lining is fixedly bonded to the surface of the clamping plate arranged on the moving seat and in contact with the liquid medicine tube. A connecting shaft seat integrally formed on one side of the clamping plate is rotatably connected to the moving seat through a rotating shaft, and a supporting spring plate is arranged between the clamping plate and the moving seat.

[0011] Further, a second lead screw is arranged in the cavity inside the housing. The two ends of the second lead screw are rotatably connected to the housing through bearings and are in transmission connection with a second servo motor. The second lead screw is in threaded connection with a threaded sleeve arranged on the sliding seat. Sliding grooves formed on both sides of the sliding seat are slidably connected to sliding rails arranged on the inner wall of the housing, and the sliding seat is slidably arranged in the inner cavity of the housing.

[0012] Further, a second sliding sleeve integrally arranged on the hanging rack is slidably connected to the support column. A fixed threaded sleeve arranged between the second sliding sleeve and the hanging rack is in threaded connection with the first lead screw. The lower end of the support column is fixedly connected to the base, and the upper end is fixedly connected to the top rack. Both ends of the first lead screw are rotatably connected to the base and the top rack at corresponding positions through bearings. The top end of the first lead screw is in transmission connection with a first servo motor on the top rack;

[0013] One end of the connecting frame is fixedly connected to the manifold box, and a first sliding sleeve integrally arranged at the other end is slidably connected to the support column. A movable threaded sleeve arranged on the connecting frame is in threaded connection with the first lead screw. The movable threaded sleeve is rotatably connected to the connecting frame through a bearing, and a driven gear fixedly connected to the movable threaded sleeve is meshed with a driving gear. The driving gear is fixedly connected to an adjustment motor, and the adjustment motor is fixedly connected to the connecting frame;

[0014] A controller arranged on the upper part of the base is electrically connected to the first servo motor, the adjustment motor, and the second servo motor.

[0015] Further, one end of the liquid outlet pipe is fixedly connected to a dropper arranged at the lower part of the liquid medicine tube, and the other end is fixedly connected to a metering solenoid valve arranged at a corresponding position on the manifold box. A liquid outlet solenoid valve arranged at the lower part of the manifold box is fixedly connected to a medicine delivery pipe, and a solenoid valve is arranged on the medicine delivery pipe.

[0016] (III) Beneficial effects

[0017] Compared with the prior art, the present invention provides an in-vivo patch clamp perfusion device, which has the following beneficial effects:

[0018] In the present invention, an adjustable frame and adjusting members are used to install and fix a liquid medicine tube for temporarily storing liquid medicine. During use, the adjusting members and the height of the clamped liquid medicine tube are directly adjusted on the frame. At the same time, the height of the liquid medicine tube can also be finely adjusted on the adjusting members, and the height of the liquid medicine tube can be adjusted in real time during the external discharge of the liquid medicine, so as to ensure that the liquid level inside the liquid medicine tube is always at an appropriate position, avoid affecting perfusion due to the decrease of the liquid level during the external discharge of the liquid medicine, ensure the stable and smooth progress of perfusion, improve the quality and efficiency of perfusion, and the overall structure is simple and easy to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic structural diagram of the present invention;

[0020] Figure 2 is a schematic structural diagram of the frame in the present invention;

[0021] Figure 3 is a schematic structural diagram of the adjusting member in the first embodiment of the present invention;

[0022] Figure 4 is a schematic structural diagram of the adjusting member in the second embodiment of the present invention;

[0023] Figure 5 is an exploded view of the adjusting member in the second embodiment of the present invention.

[0024] In the figure: 1, frame; 101, base; 102, support column; 103, first lead screw; 104, connecting frame; 1041, first sliding sleeve; 1042, movable thread sleeve; 1043, driven gear; 1044, driving gear; 1045, adjusting motor; 105, hanging frame; 1051, second sliding sleeve; 1052, fixed thread sleeve; 106, top frame; 107, first servo motor; 108, controller; 2, adjusting member; 201, housing; 202, screw; 203, adjusting block; 204, support spring; 205, moving seat; 206, clamping plate; 207, rubber inner lining; 208, sliding seat; 2081, threaded sleeve; 2082, chute; 209, second lead screw; 210, second servo motor; 211, slide rail; 212, support spring plate; 3, liquid medicine tube; 301, drip nozzle; 302, liquid outlet pipe; 4, confluence box; 401, metering solenoid valve; 402, liquid outlet solenoid valve; 403, medicine delivery pipe. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0025] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0026] Embodiment 1

[0027] As Figure 1 、 Figure 2 and Figure 3 shown, a patch clamp perfusion device in vivo proposed in an embodiment of the present invention includes a frame 1, an adjusting member 2, a liquid medicine tube 3, and a confluence box 4. An adjusting member 2 is arranged on the hanging rack 105 of the frame 1. A clamping plate 206 for clamping the liquid medicine tube 3 is arranged on the housing 201 of the adjusting member 2. The clamping plate 206 is rotatably arranged on a moving seat 205 slidably arranged on a screw rod 202. The moving seat 205 abuts against a support spring 204, which is convenient for stably installing the liquid medicine tube 3 on the frame 1 by using the adjusting member 2, thereby fixedly installing the liquid medicine tube 3. During use, the height of the adjusting member 2 and the clamped liquid medicine tube 3 can be directly adjusted on the frame 1. At the same time, the height of the liquid medicine tube 3 can be finely adjusted on the adjusting member 2, and the height of the liquid medicine tube 3 can be adjusted in real time during the external discharge of the liquid medicine, so as to ensure that the liquid level inside the liquid medicine tube 3 is always at an appropriate position, avoid the influence on perfusion caused by the decrease of the liquid level as the liquid medicine is discharged, ensure the stable and smooth progress of perfusion, improve the quality and efficiency of perfusion, and the liquid medicine tube 3 is communicated with the confluence box 4 through a liquid outlet pipe 302. The confluence box 4 is arranged on the connecting rack 104 of the frame 1. A medicine delivery pipe 403 is arranged on the confluence box 4, which can effectively confluence the liquid medicine and then discharge the liquid medicine, thus facilitating the experiment.

[0028] As Figure 1 and Figure 3As shown, in some embodiments, a hook is fixedly connected to the outer wall of the rear part of the housing 201. The hook is arranged in cooperation with the hanging bracket 105, which is convenient for stably installing the entire adjusting member 2 on the hanging bracket 105 to ensure stable cooperation between them. The upper ends of the screw rods 202 are fixedly connected to the housing 201. The support springs 204 are sleeved on the screw rods 202. The lower ends of the support springs 204 abut against the adjusting blocks 203 threadedly connected to the screw rods 202. The moving seat 205 is slidably arranged in the inner cavity of the housing 201. A through hole for slidably inserting the screw rod 202 is formed in the moving seat 205. During use, the support spring 204 provides stable and effective support for the moving seat 205 and ensures that the moving seat 205 can smoothly slide up and down on the screw rod 202. When the liquid medicine tube 3 is installed on the adjusting member 2 and liquid medicine is injected, under the action of gravity, the support spring 204 will be compressed. And as the liquid medicine slowly drains out subsequently, the weight of the liquid medicine tube 3 continuously becomes smaller, and the support spring 204 will push the moving seat 205 to slide on the screw rod 202. After the liquid level in the liquid medicine tube 3 drops as the liquid medicine drains out, the support spring 204 pushes the liquid medicine tube 3 to move on the screw rod 202, so that the liquid level remains at the same height as the initial liquid level, reducing the impact of liquid medicine drainage on perfusion, ensuring stable and smooth perfusion, improving the perfusion efficiency. At the same time, when the liquid level height in the liquid medicine tube 3 does not meet the perfusion requirements, directly use auxiliary tools to rotate the adjusting block 203 on the screw rod 202, and then use the adjusting block 203 to drive the support spring 204 and the moving seat 205 to move together on the screw rod 202, so that the height of the liquid medicine tube 3 is finely adjusted, and the liquid level height is at a suitable position for perfusion;

[0029] The moving seat 205 slidably abuts against the inner wall of the housing 201 to ensure that the moving seat 205 can smoothly slide in the housing 201 to complete the position adjustment operation and ensure smooth use. A rubber lining 207 is fixedly bonded to the surface of the clamping plate 206 arranged on the moving seat 205 and in contact with the liquid medicine tube 3, which is convenient for increasing the friction between the clamping plate 206 and the liquid medicine tube 3 and ensuring that the clamping plate 206 can stably clamp and fix the liquid medicine tube 3, achieving stable installation and fixation of the liquid medicine tube 3. One side of the clamping plate 206 is integrally formed with a connecting shaft seat that is rotatably connected to the moving seat 205 through a rotating shaft. And a support spring plate 212 is arranged between the clamping plate 206 and the moving seat 205 to ensure stable support force is provided for the clamping plate 206, so that the clamping plate 206 can stably clamp the liquid medicine tube 3, fix it in place, and then stably supply liquid medicine for perfusion operation.

[0030] As Figure 1 and Figure 2As shown, in some embodiments, the second sliding sleeve 1051 integrally provided on the hanging bracket 105 is slidably connected to the support column 102, facilitating the stable and smooth movement of the hanging bracket 105 on the frame body 1, achieving stable and smooth debugging operations. The fixed threaded sleeve 1052 provided between the second sliding sleeve 1051 and the hanging bracket 105 is threadedly connected to the first lead screw 103. The lower end of the support column 102 is fixedly connected to the base 101, and the upper end is fixedly connected to the top frame 106. Both ends of the first lead screw 103 are rotatably connected to the base 101 and the top frame 106 at corresponding positions through bearings. The top end of the first lead screw 103 is drivingly connected to the first servo motor 107 on the top frame 106, facilitating the operation of the first servo motor 107 to drive the first lead screw 103 to rotate. The first lead screw 103 drives the hanging bracket 105 to move on the frame body 1 through the fixed threaded sleeve 1052, completing the height debugging operation of the hanging bracket 105. Thus, before perfusion, according to the actual usage requirements, the height of the liquid medicine tube 3 is debugged so that the liquid level height in the liquid medicine tube 3 is at an appropriate position, improving the perfusion efficiency;

[0031] One end of the connecting bracket 104 is fixedly connected to the confluence box 4, and the first sliding sleeve 1041 integrally provided at the other end is slidably connected to the support column 102, facilitating the stable and smooth movement of the connecting bracket 104 on the frame body 1, achieving stable and smooth debugging operations, thereby driving the confluence box 4 to move and adjusting the height of the confluence box 4, improving the speed and efficiency of the external discharge and supply of the liquid medicine. The movable threaded sleeve 1042 arranged on the connecting bracket 104 is threadedly connected to the first lead screw 103, facilitating the stable connection of the connecting bracket 104 and the first lead screw 103, and realizing stable threaded transmission between the two during the adjustment process. The movable threaded sleeve 1042 is rotatably connected to the connecting bracket 104 through a bearing, and the driven gear 1043 fixedly connected to the movable threaded sleeve 1042 is meshed with the driving gear 1044. The driving gear 1044 is fixedly connected to the adjustment motor 1045, and the adjustment motor 1045 is fixedly connected to the connecting bracket 104. When it is necessary to adjust the height of the connecting bracket 104 on the frame body 1, the adjustment motor 1045 is turned on. Through the cooperation of the driving gear 1044 and the driven gear 1043, the movable threaded sleeve 1042 is driven to rotate, and then the connecting bracket 104 moves on the first lead screw 103 and the support column 102, completing the height debugging operation. At the same time, when the first lead screw 103 operates to drive the hanging bracket 105 to adjust the height, the adjustment motor 1045 also operates accordingly, driving the movable threaded sleeve 1042 to rotate on the first lead screw 103, thereby ensuring that the connecting bracket 104 does not move on the first lead screw 103 and the support column 102, avoiding interference between them and achieving stable operation and use.

[0032] As Figure 1As shown, in some embodiments, one end of the liquid outlet pipe 302 is fixedly connected to the nozzle 301 provided at the lower part of the liquid medicine pipe 3, and the other end is fixedly connected to the metering solenoid valve 401 provided at the corresponding position on the confluence box 4, facilitating the smooth diversion of the liquid medicine in the liquid medicine pipe 3 into the confluence box 4. At the same time, the flow rate of the liquid medicine is measured during the diversion process, so as to conveniently understand the perfusion situation in real time. The liquid outlet solenoid valve 402 provided at the lower part of the confluence box 4 is fixedly connected with a medicine delivery pipe 403, and a solenoid valve is arranged on the medicine delivery pipe 403, facilitating the smooth discharge of the liquid medicine in the confluence box 4 from the medicine delivery pipe 403, achieving the smooth supply of the liquid medicine and ensuring the stable use of the device.

[0033] Among them, the controller 108 arranged on the upper part of the base 101 is electrically connected to the first servo motor 107, the adjustment motor 1045, and the second servo motor 210, and is also electrically connected to the metering solenoid valve 401, the liquid outlet solenoid valve 402, and the solenoid valve on the medicine delivery pipe 403. Therefore, during the use process, various signals are received in real time, the signals are processed, and then each component of the entire device is controlled to achieve continuous and effective stable perfusion, ensuring the stable operation and use of the entire device.

[0034] Embodiment Two

[0035] As Figure 1 、 Figure 4 and Figure 5As shown in the figure, on the basis of the first embodiment, a second lead screw 209 is arranged in the cavity inside the housing 201. Both ends of the second lead screw 209 are rotatably connected to the housing 201 through bearings and are in transmission connection with the second servo motor 210, facilitating the smooth rotation of the second lead screw 209 on the housing 201 when the second servo motor 210 operates, and then driving the sliding seat 208 to slide inside the housing 201 to complete the height adjustment of the clamping plate 206. The clamped liquid medicine tube 3 moves together with the clamping plate 206 to complete the height adjustment. Moreover, the second lead screw 209 is threadedly connected to the threaded sleeve 2081 arranged on the sliding seat 208. The sliding grooves 2082 opened on both sides of the sliding seat 208 are slidably connected to the sliding rails 211 arranged on the inner wall of the housing 201, and the sliding seat 208 is slidably arranged in the inner cavity of the housing 201, facilitating the stable threaded connection of the second lead screw 209 and the sliding seat 208 together, realizing the stable threaded transmission between the two. During the driving process, the sliding seat 208 moves stably and smoothly inside the housing 201 to complete the debugging operation. During use, as the liquid medicine inside the liquid medicine tube 3 is drained away, during the process of the liquid level dropping, after the controller 108 receives the liquid medicine flow information of the metering solenoid valve 401 corresponding to the liquid medicine tube 3, it processes the signal, calculates the speed of the liquid level dropping through the specification of the liquid medicine tube 3, and then controls the corresponding second servo motor 210 to operate, driving the sliding seat 208 to slide inside the housing 201 through the second lead screw 209. Then the corresponding liquid medicine tube 3 moves together, completing the height adjustment of the liquid medicine tube 3, so that the liquid level height inside the liquid medicine tube 3 is kept consistent with the initial liquid level height, ensuring that the gravity perfusion flow rate of the liquid medicine is not affected, achieving the continuous and stable supply of the liquid medicine, and improving the perfusion efficiency.

[0036] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An in vivo patch clamp perfusion device, comprising a frame (1), an adjusting member (2), a drug liquid tube (3) and a junction box (4), characterized in that: An adjusting member (2) is mounted on the hanger (105) of the frame (1); a clamp (206) for clamping the medicine liquid tube (3) is mounted on the shell (201) of the adjusting member (2); the clamp (206) is rotatably mounted on a movable seat (205) slidably mounted on a screw rod (202); the movable seat (205) abuts against a support spring (204); the medicine liquid tube (3) is connected to a junction box (4) via a liquid outlet pipe (302); the junction box (4) is mounted on a connecting frame (104) of the frame (1); and a medicine delivery tube (403) is mounted on the junction box (4).

2. The in vivo patch clamp perfusion device according to claim 1, characterized in that: A hook is fixedly connected to the outer wall of the rear part of the shell (201), and the hook is arranged in cooperation with the hanger (105). The upper end of the screw rod (202) is fixedly connected to the shell (201), and the support spring (204) is sleeved on the screw rod (202). The lower end of the support spring (204) abuts against the adjustment block (203) threadedly connected to the screw rod (202), and the movable seat (205) is slidably arranged in the internal cavity of the shell (201), and a through hole is opened on the movable seat (205) for slidingly inserting the screw rod (202).

3. The in vivo patch clamp perfusion device according to claim 2, characterized in that: The movable seat (205) is slidably abutted against the inner wall of the shell (201); a clamping plate (206) arranged on the movable seat (205) is fixedly bonded with a rubber lining (207) on one side in contact with the medicine liquid tube (3); a connecting shaft seat integrally formed on one side of the clamping plate (206) is rotatably connected to the movable seat (205) via a rotating shaft; and a supporting spring plate (212) is arranged between the clamping plate (206) and the movable seat (205).

4. The in vivo patch clamp perfusion device according to claim 1, characterized in that: A second screw rod (209) is arranged in the cavity inside the housing (201), and both ends of the second screw rod (209) are rotatably connected to the housing (201) through bearings and are transmission-connected to the second servo motor (210), and the second screw rod (209) is threadedly connected to a threaded sleeve (2081) arranged on the sliding seat (208), and the sliding grooves (2082) opened on both sides of the sliding seat (208) are slidably connected to the sliding rails (211) arranged on the inner wall of the housing (201), and the sliding seat (208) is slidably arranged in the internal cavity of the housing (201).

5. The in vivo patch clamp perfusion device according to claim 1, characterized in that: A second sliding sleeve (1051) integrally arranged on the hanging bracket (105) is slidably connected to the support column (102), a fixed threaded sleeve (1052) arranged between the second sliding sleeve (1051) and the hanging bracket (105) is threadedly connected to the first screw rod (103), and the lower end of the support column (102) is fixedly connected to the base (101), and the upper end is fixedly connected to the top frame (106), and both ends of the first screw rod (103) are rotatably connected to the base (101) and the top frame (106) at corresponding positions through bearings, and the top end of the first screw rod (103) is drivingly connected to the first servo motor (107) on the top frame (106).

6. The in vivo patch clamp perfusion device according to claim 5, characterized in that: One end of the connecting frame (104) is fixedly connected to the junction box (4), and the other end of the first sliding sleeve (1041) is integrally arranged and slidably connected to the support column (102), and a movable threaded sleeve (1042) arranged on the connecting frame (104) is threadedly connected to the first screw rod (103), the movable threaded sleeve (1042) is rotatably connected to the connecting frame (104) through a bearing, and a driven gear (1043) fixedly connected to the movable threaded sleeve (1042) is meshedly connected to a driving gear (1044), the driving gear (1044) is fixedly connected to an adjusting motor (1045), and the adjusting motor (1045) is fixedly connected to the connecting frame (104).

7. The in vivo patch clamp perfusion device according to claim 5, characterized in that: The controller (108) disposed on the upper portion of the base (101) is electrically connected to the first servo motor (107), the adjustment motor (1045) and the second servo motor (210).

8. The in vivo patch clamp perfusion device according to claim 1, characterized in that: One end of the liquid outlet pipe (302) is fixedly connected to a drip nozzle (301) arranged at the lower part of the liquid medicine pipe (3), and the other end is fixedly connected to a metering electromagnetic valve (401) arranged at a corresponding position on the junction box (4); the liquid outlet electromagnetic valve (402) arranged at the lower part of the junction box (4) is fixedly connected to a drug delivery pipe (403), and a solenoid valve is arranged on the drug delivery pipe (403).

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