Cell biology experiment sample culture equipment

Abstract

The invention relates to the technical field of enzymology or microbiology devices, in particular to cell biology experiment sample culture equipment which comprises an equipment body, the equipment body at least comprises a box body, a door stop, a handle and a cover body, an observation window is formed in the door stop, the handle is fixedly connected to the surface of the door stop, the door stop is rotationally connected to one side of the box body, and the cover body is fixedly connected to the box body. The cover body is fixedly mounted on the peripheral side of the box body, a temperature controller, a placement body group and a controller are arranged in the box body, the controller controls the placement body group, and the temperature controller regulates and controls the internal temperature of the box body. The problem that in the prior art, due to the fact that the temperature error is large, the cell culture effect is poor, and even the failure rate is high is solved.

Description

Technical Field

[0001] This application relates to the technical field of enzymology or microbiology devices, and more particularly, to a cell biology experiment sample culture device. Background Art

[0002] Temperature directly affects the metabolic rate of cells. Within an appropriate temperature range, cells are metabolically active and can efficiently utilize nutrients for growth and reproduction. When the temperature is too high or too low, cell metabolism slows down, resulting in a reduced growth rate and even possible cell death.

[0003] The Chinese patent with the published patent name of "A Cell Biology Experiment Sample Culture Device" and the publication number CN212025377U can achieve cell culture, but it cannot precisely control the temperature, resulting in poor cell culture effects or even a high failure rate due to large temperature errors during cell culture. Summary of the Invention

[0004] The purpose of this application is to provide a cell biology experiment sample culture device, which solves the problem of poor cell culture effects or even a high failure rate due to large temperature errors in the prior art, and at the same time provides a device that can achieve full fusion of cultured cells with the surrounding nutrient solution.

[0005] The technical solution of this application:

[0006] This application provides a cell biology experiment sample culture device, including a device body, which at least includes a box body, a door stop, a handle, and a cover body. An observation window is opened on the door stop, the handle is fixedly connected to the surface of the door stop, the door stop is rotatably connected to one side of the box body, the cover body is fixedly installed on the periphery of the box body, and the box body includes a thermostat, a placement body group, and a controller. The controller controls the placement body group, and the thermostat regulates the temperature inside the box body.

[0007] In some embodiments, the thermostat at least includes a first thermal controller, a second thermal controller, and a third thermal controller, and a temperature precision control system is configured in each of the first thermal controller, the second thermal controller, and the third thermal controller.

[0008] In some embodiments, the temperature precision control system at least includes a temperature difference detection module, a hierarchical compensation module, a temperature recheck module, a warning module, and a processor; among them, when the temperature difference detection module detects that the temperature inside the box body is different from the set culture temperature, it will upload the temperature data to the processor, and the processor activates the hierarchical compensation module for temperature compensation. The temperature recheck module is data-connected to the hierarchical compensation module, and the temperature recheck module will perform a recheck on the compensated temperature. The warning module is data-connected to the temperature recheck module, and when the recheck result of the temperature recheck module is incorrect, the warning module will issue a warning prompt.

[0009] In some embodiments, the processor is data - connected to the warning module. When the processor receives abnormal data from the temperature difference detection module, it will control the warning module to execute a preliminary warning prompt.

[0010] In some embodiments, the hierarchical compensation module at least includes a temperature difference primary compensation module, a temperature difference secondary compensation module, and a temperature difference tertiary compensation module that are interconnected.

[0011] In some embodiments, the thermostat further at least includes a first heat transfer channel, a second heat transfer channel, and a third heat transfer channel that are arranged in parallel at intervals in sequence. Among them, the first heat transfer channel, the second heat transfer channel, and the third heat transfer channel respectively transfer the output temperatures of the first thermostat, the second thermostat, and the third thermostat.

[0012] In some embodiments, the placement body group at least includes a first placement body and a second placement body, and the first placement body is arranged parallel to the second placement body.

[0013] In some embodiments, a driving motor and a connecting seat for fixing the driving motor are arranged inside the cover. A driving rod is fixedly connected to the output end of the driving motor. The driving rod is fixedly connected to a main connecting rod. The main connecting rod is fixedly connected to the second placement body. The second placement body is fixedly connected to an auxiliary connecting rod, and the auxiliary connecting rod is fixedly connected to the first placement body.

[0014] In some embodiments, the main connecting rod includes a short rod and a long rod. One end of the short rod is fixedly connected to the driving rod, and the other end of the short rod is rotatably connected to the long rod.

[0015] In some embodiments, the controller includes a display screen, a control console, a first slide rail, and a second slide rail. Among them, the display screen is data - connected to the control console. The control console controls the driving motor. The first slide rail and the second slide rail cooperate to realize the pulling out and retracting of the controller relative to the box body.

[0016] The technical solution of this application at least has the following advantages and beneficial effects: The precise control of the temperature of the cell culture environment is achieved through the thermostat and the controller, and the temperature constancy in the cell culture environment is preferably realized, solving the problem in the prior art that the cell culture effect is poor or the failure rate is high due to large temperature errors. By setting the placement body group to swing the cell culture carrier, it is possible to ensure the fullest contact between the cells and the culture solution to the greatest extent, solving the problem in the prior art that the cultured cells cannot be fully fused with the surrounding nutrient solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a structural schematic diagram of this application;

[0018] Figure 2 is a structural schematic diagram inside this application;

[0019] Figure 3 Another structural schematic diagram inside this application;

[0020] Figure 4 Structural schematic diagram of the side of this application;

[0021] Figure 5 Another structural schematic diagram inside this application;

[0022] Figure 6 Structural schematic diagram of the temperature precision control system in this application.

[0023] In the figure: 100 - equipment body; 1 - box body; 2 - door stop; 3 - handle; 4 - cover body; 5 - observation window; 6 - controller; 7 - first thermal controller; 8 - second thermal controller; 9 - third thermal controller; 10 - first heat transfer channel; 11 - second heat transfer channel; 12 - third heat transfer channel; 13 - first placement body; 14 - second placement body; 15 - drive motor; 16 - connecting seat; 17 - drive rod; 18 - main connecting rod; 19 - auxiliary connecting rod; 20 - display screen; 21 - control console; 22 - first slide rail; 23 - second slide rail. Specific implementation manners

[0024] Next, the technical solutions in the embodiments of this application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of this application. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of this application.

[0025] Embodiment

[0026] Please refer to together Figures 1-6 , this application provides a cell biology experiment sample culture device, including an equipment body 100. The equipment body 100 at least includes a box body 1, a door stop 2, a handle 3, and a cover body 4. An observation window 5 is opened on the door stop 2. The handle 3 is fixedly connected to the surface of the door stop 2. The door stop 2 is rotatably connected to one side of the box body 1. The cover body 4 is fixedly installed on the periphery of the box body 1. The inside of the box body 1 includes a temperature controller, a placement body group, and a controller 6. The controller 6 controls the placement body group, and the temperature controller regulates the temperature inside the box body 1.

[0027] It should be noted that in this embodiment, the box body 1 is connected to the door stop 2, the handle 3, the cover body 4, the observation window 5, the temperature controller, the controller 6, and the placement body group. The cover body 4 and the door stop 2 are provided to achieve the airtightness of the entire cell biological culture environment. The handle 3 is used to open the entire box body 1 and take out and place the cultured cells. The observation window 5 is used to directly view the culture state of the cells in the airtight culture lens. The temperature controller and the controller 6 are used to accurately control the temperature of the cell culture environment, preferably achieving a constant temperature in the cell culture environment, and solving the problem in the prior art that due to a large temperature error, the effect of cell culture is poor or even the failure rate is high. Further, the placement body group is provided to swing the cell culture carrier, maximizing the guarantee of sufficient contact between the cells and the culture solution, and solving the problem in the prior art that the cultured cells and the surrounding nutrient solution cannot be fully fused.

[0028] Further, the temperature controller at least includes a first thermal controller 7, a second thermal controller 8, and a third thermal controller 9, and a temperature precision control system is configured in each of the first thermal controller 7, the second thermal controller 8, and the third thermal controller 9. The first thermal controller 7, the second thermal controller 8, and the third thermal controller 9 are used to achieve separate or synchronous temperature control and heat transfer, and thus can achieve multi-level temperature control, and also ensure that the temperature in the cell culture space can always be regulated, and at the same time ensure that sufficient heat can always be provided when heat is required.

[0029] Further, the temperature precision control system at least includes a temperature difference detection module, a grading compensation module, a temperature recheck module, a warning module, and a processor; wherein, when the temperature difference detection module detects that the temperature in the box body 1 is different from the set culture temperature, it will upload the temperature data to the processor, and the processor activates the grading compensation module to perform temperature compensation. The temperature recheck module is data-connected to the grading compensation module, and the temperature recheck module will perform a recheck on the compensated temperature. The warning module is data-connected to the temperature recheck module. When the recheck result of the temperature recheck module is incorrect, the warning module will issue a warning prompt. Through the temperature difference detection module, the grading compensation module, the temperature recheck module, the warning module, and the processor included in the temperature precision control system, the control and adjustment of the first thermal controller 7, the second thermal controller 8, and the third thermal controller 9 are achieved. Specifically, when the temperature recheck module issues a recheck result error signal, the temperature difference detection module performs temperature detection again, and the grading compensation module performs temperature compensation until the temperature in the cell culture space is adjusted to the specified temperature. The grading compensation module is connected to each of the first thermal controller 7, the second thermal controller 8, and the third thermal controller 9, and thus the temperature adjustment is achieved.

[0030] Further, the processor is data-connected to the warning module. When the processor receives abnormal data from the temperature difference detection module, it will control the warning module to execute a preliminary warning prompt. Through warning prompts of different levels, the staff can take corresponding measures in a targeted manner, enabling them to respond to problems in the shortest possible time, reducing the failure rate of cell culture, and at the same time reducing the cost of the entire work.

[0031] Further, the hierarchical compensation module at least includes a temperature difference first-level compensation module, a temperature difference second-level compensation module, and a temperature difference third-level compensation module that are interconnected. The temperature difference first-level compensation module is data-connected to the temperature difference second-level compensation module, the temperature difference first-level compensation module is data-connected to the temperature difference third-level compensation module, and the temperature difference second-level compensation module is data-connected to the temperature difference third-level compensation module, enabling multiple temperature combination methods to be available when abnormal temperature occurs, ensuring that all temperatures to be compensated can be compensated and adjusted, and further solving the problem in the prior art that due to large temperature errors, the effect of cell culture is poor or even the failure rate is high.

[0032] Further, the thermostat further at least includes a first heat transfer channel 10, a second heat transfer channel 11, and a third heat transfer channel 12 that are sequentially arranged at intervals and in parallel. Among them, the first heat transfer channel 10, the second heat transfer channel 11, and the third heat transfer channel 12 respectively transfer the output temperatures of the first thermostat 7, the second thermostat 8, and the third thermostat 9. The first heat transfer channel 10, the second heat transfer channel 11, and the third heat transfer channel 12 realize the supplementation or adjustment of the temperature in the cell culture space through multiple heat transfer paths. If there is only one heat transfer path, it is possible that the entire cell culture space cannot complete the temperature adjustment due to the failure of this path. Compared with only one path, the multiple heat transfer paths in this embodiment can work independently or simultaneously, ensuring that the temperature in the entire cell culture space can always be adjusted, and thus ensuring the normal progress of cell culture work.

[0033] Further, the placement body group at least includes a first placement body 13 and a second placement body 14, and the first placement body 13 is arranged parallel to the second placement body 14. By providing the first placement body 13 and the second placement body 14, more cell carriers can be placed, increasing the number of cells cultured, increasing the number of successfully cultured cells, providing a sufficient number of samples for trial and error for subsequent work after cell culture, and thus ensuring the normal progress of subsequent work, solving the problem in the prior art that although cell culture can be achieved, due to the excessive number of failed cultured cells and the small number of successful ones, there are not enough experimental samples for subsequent work, resulting in the inability to carry out subsequent work normally.

[0034] Further, a driving motor 15 and a connecting seat 16 for fixing the driving motor 15 are arranged inside the cover body 4. The output end of the driving motor 15 is fixedly connected with a driving rod 17. The driving rod 17 is fixedly connected with a main connecting rod 18. The main connecting rod 18 is fixedly connected with a second placing body 14. The second placing body 14 is fixedly connected with an auxiliary connecting rod 19. The auxiliary connecting rod 19 is fixedly connected with a first placing body 13. Specifically, the connection and fixation between the driving motor 15 and the box body 1 are realized through the connecting seat 16. During operation, the driving motor 15 rotates to drive the driving rod 17 to rotate, thereby driving the main connecting rod 18 to rotate around the driving rod 17, the second placing body 14 to swing, the auxiliary connecting rod 19 to swing, and the first placing body 13 to swing, realizing the synchronous swinging of the second placing body 14 and the first placing body 13, and further realizing the full contact between the cells and the culture medium.

[0035] Further, the main connecting rod 18 includes a short rod and a long rod. One end of the short rod is fixedly connected with the driving rod 17, and the other end of the short rod is rotatably connected with the long rod. Specifically, the end of the long rod away from the short rod is connected to the end of the side of the second placing body 14 close to the door stop 2. One end of the auxiliary connecting rod 19 is connected to the front section of the side of the second placing body 14, and the other end is connected to the front section of the side of the first placing body 13. The middle parts of both sides of the second placing body 14 and the first placing body 13 are respectively rotatably connected with the inner wall of the box body 1 through L-shaped connecting pieces.

[0036] Further, the controller 6 includes a display screen 20, a control console 21, a first slide rail 22, and a second slide rail 23. The display screen 20 is data-connected to the control console 21. The control console 21 controls the driving motor 15. The first slide rail 22 and the second slide rail 23 cooperate to realize the pulling out and retracting of the controller 6 relative to the box body 1. By pulling the controller 6 outwards or pushing it inwards along the first slide rail 22, the position of the controller 6 is changed.

[0037] Cell samples to be cultured are pre-placed in the first placing body 13 and the second placing body 14 in the equipment of this application. At this time, the door stop 2 is closed, and the construction of the culture environment is started through the controller 6. At this time, the driving motor 15 drives the driving rod 17, the driving rod 17 drives the main connecting rod 18, the main connecting rod 18 drives the second placing body 14, the second placing body 14 drives the auxiliary connecting rod 19, and the auxiliary connecting rod 19 drives the first placing body 13 to realize the front-back micro-angle swing of the first placing body 13 and the second placing body 14. Then, the temperature controller will also control the first thermal controller 7, the second thermal controller 8, and the third thermal controller 9 under the operation of the controller 6, and its control process needs to be realized through the temperature precision control system. Finally, the equipment body 100 realizes the culture of the cell culture sample through the temperature control inside the box body 1 and the swing of the first placing body 13 and the second placing body 14.

[0038] Although some specific embodiments of the present disclosure have been described in detail by way of examples, those skilled in the art should understand that the above examples are only for illustration purposes and not for limiting the scope of the present disclosure. Those skilled in the art should understand that the above embodiments can be modified or some technical features can be equivalently replaced without departing from the scope and spirit of the present disclosure. The scope of the present disclosure is defined by the appended claims.

Claims

1. A cell biology experimental sample culture device, characterized in that: The device comprises a device body (100), wherein the device body (100) comprises at least a box body (1), a door stop (2), a handle (3) and a cover body (4); an observation window (5) is provided on the door stop (2); the handle (3) is fixedly connected to the surface of the door stop (2); the door stop (2) is rotatably connected to one side of the box body (1); the cover body (4) is fixedly installed on the peripheral side of the box body (1); the box body (1) comprises a thermostat, a placement body group and a controller (6); the controller (6) controls the placement body group; and the thermostat regulates the internal temperature of the box body (1).

2. The culture device according to claim 1, characterized in that: The temperature controller comprises at least a first thermal controller (7), a second thermal controller (8) and a third thermal controller (9), and each of the first thermal controller (7), the second thermal controller (8) and the third thermal controller (9) is provided with a temperature precision control system.

3. The culture device according to claim 2, characterized in that: The temperature precision control system at least includes a temperature difference detection module, a graded compensation module, a temperature recheck module, a warning module and a processor; When the temperature difference detection module detects that the temperature in the box (1) is different from the set culture temperature, the temperature data will be uploaded to the processor, and the processor will activate the graded compensation module to perform temperature compensation. The temperature recheck module is data-connected to the graded compensation module, and the temperature recheck module will recheck the compensated temperature. The warning module is data-connected to the temperature recheck module, and when the recheck result of the temperature recheck module is wrong, the warning module will issue a warning prompt.

4. The culture device according to claim 3, characterized in that: The processor is connected to the warning module data, and when the processor receives abnormal data from the temperature difference detection module, it controls the warning module to execute a preliminary warning prompt.

5. The culture device according to claim 3, characterized in that: The hierarchical compensation module at least includes a primary temperature difference compensation module, a secondary temperature difference compensation module and a tertiary temperature difference compensation module which are interconnected.

6. The culture device according to claim 2, characterized in that: The temperature controller further comprises at least a first heat transfer channel (10), a second heat transfer channel (11) and a third heat transfer channel (12) which are arranged in parallel and spaced apart in sequence, wherein the first heat transfer channel (10), the second heat transfer channel (11) and the third heat transfer channel (12) respectively transmit the output temperatures of the first thermal controller (7), the second thermal controller (8) and the third thermal controller (9).

7. The culture device according to claim 1, characterized in that: The placement body group comprises at least a first placement body (13) and a second placement body (14), wherein the first placement body (13) is arranged parallel to the second placement body (14).

8. The culture device according to claim 7, characterized in that: A driving motor (15) and a connecting seat (16) for fixing the driving motor (15) are arranged in the cover body (4); the output end of the driving motor (15) is fixedly connected to a driving rod (17); the driving rod (17) is fixedly connected to a main connecting rod (18); the main connecting rod (18) is fixedly connected to the second placement body (14); the second placement body (14) is fixedly connected to an auxiliary connecting rod (19); and the auxiliary connecting rod (19) is fixedly connected to the first placement body (13).

9. The culture device according to claim 8, characterized in that: The main connecting rod (18) comprises a short rod and a long rod, one end of the short rod is fixedly connected to the driving rod (17), and the other end of the short rod is rotatably connected to the long rod.

10. The culture device according to claim 8, characterized in that: The controller (6) comprises a display screen (20), a control console (21), a first slide rail (22) and a second slide rail (23), wherein the display screen (20) is data-connected to the control console (21), the control console (21) controls the drive motor (15), and the first slide rail (22) and the second slide rail (23) cooperate to realize the pulling out and retracting of the controller (6) relative to the box (1).

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