Culture device for enhancing treatment of osteoarthritis by using mesenchymal stem cells

Abstract

The invention discloses a culture device for enhancing treatment of osteoarthritis by using mesenchymal stem cells, and relates to the technical field of mesenchymal stem cell culture. The top of the box body is opened and is provided with a box cover for covering, and the side surface of the box body is provided with a box door for opening and closing; a mixing box, a movable liquid dropping structure and a placing table are sequentially arranged in the box body from top to bottom; the box cover is provided with a through discharging port I and a through discharging port II, and the mixing box is connected with the discharging port I and the discharging port II; a discharging pipe is arranged at the bottom of the mixing box, and an electromagnetic valve is arranged on the discharging pipe; the movable liquid dropping structure is fixedly arranged in the box body, the movable liquid dropping structure is provided with a liquid dropping pipe, the top of the liquid dropping pipe is provided with a connecting pipe connected with the discharging pipe, and the bottom of the liquid dropping pipe is provided with a micro flow meter. According to the culture device for enhancing the treatment of the osteoarthritis by the mesenchymal stem cells, the prepared culture solution can be fully stirred and uniformly mixed, and the mesenchymal stem cells can be cultured by accurately dripping the set required culture solution in a timed and quantitative manner.

Description

Technical Field

[0001] The present invention relates to the technical field of mesenchymal stem cell culture, and more specifically, it relates to a culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells. Background Art

[0002] Osteoarthritis (OA) is the most common joint disease mainly characterized by damage to articular cartilage, and ultimately resulting in articular cartilage degeneration, fibrosis, rupture, defect and damage to the entire articular surface, involving the entire joint tissue. Mesenchymal stem cells are a type of pluripotent stem cells, which have all the common characteristics of stem cells, namely self-renewal and multi-directional differentiation ability. It is also the most widely used in clinical applications. When combined with hematopoietic stem cells, it can improve the success rate of transplantation and accelerate hematopoietic reconstruction. Mesenchymal stem cells are clinically applied to solve a variety of hematological diseases, cardiovascular diseases, liver cirrhosis, nervous system diseases, repair of partial resection injury of knee meniscus, etc. At present, the treatment of osteoarthritis still mainly focuses on joint replacement. Under specific induction conditions in vitro or in a specific in vivo environment, bone marrow mesenchymal stem cells can differentiate into tissues such as bone, cartilage, fat, muscle, bone marrow stroma, tendon and ligament. With the development of tissue engineering and the in-depth study of mesenchymal stem cells, the technology of directional differentiation of bone marrow mesenchymal stem cells into cartilage tissue has matured.

[0003] The utility model with the existing publication number CN209537507U discloses a culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells, including an incubator and a culture solution providing device; on the opposite inner walls of the incubator, a first clamping device and a second clamping device are respectively arranged from top to bottom in sequence. A first enzyme-labeled plate is clamped between the two first clamping devices, and a second enzyme-labeled plate is clamped between the two second clamping devices. Its beneficial effects are: by setting the incubator, the purpose of culturing mesenchymal stem cells can be achieved; by setting the first clamping device and the second clamping device, the purpose of clamping the enzyme-labeled plate for culturing mesenchymal stem cells can be achieved; by setting the culture solution providing device, the purpose of providing culture solution can be achieved.

[0004] However, the mixing uniformity of the culture solution of this device has certain limitations. In addition, directly spraying the culture solution from the first branch pipe or the second branch pipe will cause some culture solution not to accurately enter the corresponding wells of the enzyme-labeled plate, resulting in waste of the culture solution and uneven reception of the culture solution by mesenchymal stem cells in each well. At the same time, the amount of the culture solution entering the corresponding wells of the enzyme-labeled plate cannot be accurately controlled, which may cause the failure of culturing mesenchymal stem cells. Therefore, to solve the above technical problems, the present invention provides a culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells. Summary of the Invention

[0005] The object of the present invention is to provide a culture device for enhancing the treatment of osteoarthritis by mesenchymal stem cells, which can uniformly mix the culture solution and quantitatively and accurately add it into the corresponding wells of an enzyme-linked immunosorbent assay (ELISA) plate for culturing mesenchymal stem cells.

[0006] The above technical object of the present invention is achieved through the following technical solutions: A culture device for enhancing the treatment of osteoarthritis by mesenchymal stem cells, comprising a box body, the top of the box body is open and provided with a coverable box cover, and a switchable box door is provided on the side of the box body; inside the box body, there are successively arranged from top to bottom a mixing box, a moving dropping structure, and a placement table; the box cover is provided with a penetrating material feeding port one and material feeding port two, and the mixing box is connected to the material feeding port one and material feeding port two; a discharge pipe is provided at the bottom of the mixing box, and an electromagnetic valve is provided on the discharge pipe; the moving dropping structure is fixedly arranged inside the box body, the moving dropping structure is provided with a dropping pipe, the top of the dropping pipe is provided with a connecting pipe connected to the discharge pipe, and a micro flowmeter is provided at the bottom of the dropping pipe; the placement table is provided with a control box and a placement area for placing an ELISA plate; a display and control device is provided on the front of the box door.

[0007] The present invention is further configured as follows: The top of the mixing box is provided with a feeding port one, a feeding port two, and a sunken installation groove, the feeding port one and the feeding port two are respectively connected to the material feeding port one and material feeding port two of the box cover; a stirring motor is arranged in the installation groove, the output end of the stirring motor is provided with a rotating rod two, the rotating rod two penetrates through the top of the mixing box, and the rotating rod two is provided with stirring rods.

[0008] The present invention is further configured as follows: An installation ring is arranged inside the box body, and a plurality of support blocks are arranged on the inner wall of the box body on the same horizontal plane, the installation ring is fixedly arranged on the support blocks, and the mixing box is detachably installed on the installation ring.

[0009] The present invention is further configured as follows: The moving dropping structure includes a moving table, a moving plate, an external motor, and two parallel threaded rods; the external motor is arranged outside the box body, the output end of the external motor is provided with a rotating rod one, the rotating rod penetrates through the box body and is provided with a runner; the two parallel threaded rods penetrate through the moving table and are threadedly connected to the moving table, the two ends of the threaded rods are rotatably connected to the box body, a runner is arranged near the end of one end of the threaded rod close to the external motor, and a belt is arranged between the runners of the two parallel threaded rods and the runner of the rotating rod one to connect them, so that the rotating rod one drives the two threaded rods to rotate through the belt; a meshing groove is arranged on the top of the moving table, and a tooth surface is arranged in the meshing groove; sliding grooves are arranged on both sides of the meshing groove, and sliding rods are arranged in the sliding grooves; the moving plate is located on the top of the moving table and is meshingly and slidably connected to the moving table; a clamping structure is arranged on the side of the moving plate for clamping the dropping pipe.

[0010] The present invention is further configured as follows: a through engaging opening is provided in the middle of the moving plate, an in-built motor is provided on the top of the moving plate, a gear is provided at the output end of the in-built motor, and the gear is located within the engaging opening and meshes with the tooth surface within the engaging groove of the moving table; a sliding seat is provided at the bottom of the moving seat, and the sliding seat is slidably connected to the sliding rod; a housing is further provided on the top of the moving plate to protect the in-built motor and the gear; a groove is provided on the side surface of the moving plate, and the clamping structure includes a moving clamp body, a fixed clamp body and a second turning handle. The moving clamp body and the fixed clamp body are located within the groove, and the second turning handle is located on the side surface adjacent to the groove and is provided with a rotating shaft passing through the moving plate and threadedly connected to the moving clamp body.

[0011] The present invention is further configured as follows: a rotating seat is provided at the bottom of the box body, and the placing table is installed on the rotating seat; fixing seats are oppositely provided on the top of the placing table, and a moving groove is provided below the placing table and within the fixing seats; the fixing seats are provided with a first turning handle having a threaded portion, and the first turning handle is provided with a pressing member, and the pressing member is located within the moving groove.

[0012] The present invention is further configured as follows: a magnet is provided on the side surface of the placing table, and the box door is made of a metallic material.

[0013] The present invention is further configured as follows: the pressing member includes symmetrically arranged moving push rods, a threaded hole member is provided on the top of the moving push rod and is threadedly connected to the first turning handle, and a pressing plate is provided at the end of the moving push rod.

[0014] The present invention is further configured as follows: ear plates are oppositely provided on the side surface of the box body near the top, and supporting feet are provided at the bottom of the box body.

[0015] The present invention is further configured as follows: a handle is provided on the top of the box cover, and cover bodies one and two for covering are respectively provided at the first material discharge opening and the second material discharge opening.

[0016] In summary, the present invention has the following beneficial effects: 1. The culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells of the present invention fully stirs the raw materials in the mixing tank through a stirring motor driving a stirring rod, making the mixture more uniform. 2. The culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells of the present invention enables the dropper tube to accurately drop the culture medium into each culture unit on the ELISA plate through the movement of the mobile dropping structure. 3. The culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells of the present invention has a placement table installed on a rotating seat, enabling the placement table to rotate, which is convenient for taking the ELISA plate. By installing magnets on the side of the placement table and making the cabinet door of metal material. After the cabinet door is closed, the rotation of the placement table can be restricted by the magnets to prevent the placement table from rotating, and at the same time, the cabinet door can be tightly closed. 4. The culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells of the present invention is provided with a micro flow meter at the bottom of the dropper tube, and the micro flow meter can accurately monitor and control the amount of nutrient solution dropped each time, enabling the set amount of culture medium to be dropped regularly and quantitatively. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic diagram of the culture device in an embodiment of the present invention;

[0018] Figure 2 is a schematic diagram of the interior of the culture device in an embodiment of the present invention;

[0019] Figure 3 is a front view of the internal structure of the culture device in an embodiment of the present invention;

[0020] Figure 4 is a schematic diagram of the mixing tank structure of the culture device in an embodiment of the present invention;

[0021] Figure 5 is a schematic diagram of the mobile dropping structure of the culture device in an embodiment of the present invention;

[0022] Figure 6 is a schematic diagram of the moving plate of the mobile dropping structure in an embodiment of the present invention;

[0023] Figure 7 is a schematic diagram of the placement table of the culture device in an embodiment of the present invention;

[0024] Figure 8 is a schematic diagram of the extrusion member in an embodiment of the present invention.

[0025] In the figure: 1. Box body; 2. Ear plate; 3. Box cover; 4. Box door; 5. Display and control device; 6. Cover body one; 7. Cover body two; 8. External motor; 9. Mixing box; 10. Installation ring; 11. Placing table; 12. Enzyme-labeled plate; 13. Magnet; 14. Rotating handle one; 15. Dropping tube; 16. Control box; 17. Connecting pipe; 18. Support block; 19. Electromagnetic valve; 20. Rotating seat; 21. Shell; 22. Rotating rod one; 23. Moving table; 24. Stirring motor; 25. Rotating rod two; 26. Stirring rod; 27. Feeding port one; 28. Feeding port two; 29. Installation groove; 30. Built-in motor; 31. Gear; 32. Moving plate; 33. Threaded rod; 34. Slide bar; 35. Meshing groove; 36. Belt; 37. Sliding seat; 38. Moving clamping body; 39. Fixed clamping body; 40. Rotating handle two; 41. Meshing port; 42. Extrusion part; 43. Fixed seat; 44. Moving groove; 45. Handle; 46. Support leg; 47. Micro flowmeter; 421. Moving push rod; 422. Threaded hole part; 423. Extrusion plate. Detailed implementation mode

[0026] The following will further elaborate on the present invention in conjunction with the attached Figure 1-8 drawings.

[0027] Embodiment: A culture device for enhancing the treatment of osteoarthritis with mesenchymal stem cells, as Figure 1 - Figure 8 shown, includes a box body 1. The top of the box body 1 is open and is provided with a box cover 3 that is closed. The side of the box body 1 is provided with an openable and closable box door 4. Inside the box body 1, there are successively arranged from top to bottom a mixing box 9, a mobile dropping structure, and a placing table 11. The box cover 3 is provided with a through feeding port one and feeding port two. The mixing box 9 is connected to the feeding port one and feeding port two. The bottom of the mixing box 9 is provided with a discharge pipe, and an electromagnetic valve 19 is provided on the discharge pipe. The mobile dropping structure is fixedly arranged inside the box body 1. The mobile dropping structure is provided with a dropping tube 15. The top of the dropping tube 15 is provided with a connecting pipe 17 connected to the discharge pipe, and the bottom of the dropping tube 15 is provided with a micro flowmeter 47. The placing table 11 is provided with a control box 16 and a placing area for placing an enzyme-labeled plate 12. The front of the box door 4 is provided with a display and control device 5. On the side of the box body 1 near the top, there are relatively arranged ear plates 2, and support legs 46 are arranged at the bottom of the box body 1. The top of the box cover 3 is provided with a handle 45, and the feeding port one and feeding port two are respectively provided with cover bodies one 6 and cover bodies two 7 that are closed.

[0028] By adopting the above technical solution, the top of the box body 1 is opened and a box cover 3 that can be closed is provided. The box cover 3 is tightly and sealingly connected to the box body 1. By providing the closable box cover 3, it is convenient to clean and maintain the mixing box 9 of the culture device in the later stage. A box door 4 that is rotatably connected and opened and closed is provided on the side of the box body 1. The enzyme-labeled plate 12 cultured with mesenchymal stem cells is taken and placed into the box body 1 through the box door 4. The placement table 11 is installed at the bottom inside the box body 1 and is used to fixedly place the enzyme-labeled plate 12 cultured with mesenchymal stem cells. The mobile dropping structure is arranged between the placement table 11 and the mixing box 9. A dropping tube 15 is clamped on the mobile dropping structure. Through the movement of the mobile dropping structure, the dropping tube 15 can accurately drop the culture solution into each culture unit on the enzyme-labeled plate 12. A display and control device 5 is provided on the front of the box door 4. The display and control device 5 includes a display and several function buttons, which can display information such as culture time, temperature and humidity, and at the same time, the amount of the culture solution dropped each time can be controlled and set through the function buttons. During use, the enzyme-labeled plate 12 cultured with mesenchymal stem cells is fixedly placed on the placement table 11, and the raw materials for preparing the culture solution are added into the mixing box 9 through the feeding port 1 and feeding port 2 of the box cover 3 and stirred and mixed evenly. The dropping amount and the interval time of the nutrient solution are set through the display and control device 5. Under the operation of the mobile dropping structure, the dropping tube 15 drops the solution into each culture unit of the enzyme-labeled plate 12. The micro flowmeter 47 can accurately monitor and control the amount of the nutrient solution dropped each time. The feeding port 1 and the feeding port 2 are respectively provided with closable cover bodies 1 and 2, which can effectively prevent pollutants in the air from entering the mixing box 9 and contaminating the prepared culture solution. A control circuit board is provided in the control box 16 to control various motors, the display and control device 5, the solenoid valve 19 and the micro flowmeter 47.

[0029] On the top of the mixing box 9, there are a feeding port 1 27, a feeding port 2 28 and a sunken installation groove 29. The feeding port 1 27 and the feeding port 2 28 are respectively connected to the feeding port 1 and the feeding port 2 of the box cover 3; a stirring motor 24 is provided in the installation groove 29. A rotating rod 2 25 is provided at the output end of the stirring motor 24. The rotating rod 2 25 penetrates through the top of the mixing box 9, and a stirring rod 26 is provided on the rotating rod 2 25. An installation ring 10 is provided inside the box body 1. A number of support blocks 18 are provided on the inner wall of the box body 1 on the same horizontal plane. The installation ring 10 is fixedly arranged on the support blocks 18, and the mixing box 9 is detachably installed on the installation ring 10.

[0030] By adopting the above technical solution, the mixing tank 9 is connected to the discharge port one and the discharge port two of the tank cover 3 through the feed port one 27 and the feed port two 28 at the top respectively to add the prepared raw materials into the mixing tank 9, and the stirring rod 26 is driven by the stirring motor 24 to be fully stirred and evenly mixed. A number of fixedly connected support blocks 18 are arranged at intervals on the inner wall of the box body 1 on the same horizontal plane, the mounting ring 10 is fixedly connected to the top of the support block 18, and the mixing tank 9 is detachably mounted on the top of the mounting ring 10 by screws. It is convenient to disassemble, clean and repair the mixing tank 9 in the later stage. A discharge pipe is provided at the bottom of the mixing tank 9, and an electromagnetic valve 19 is provided on the discharge pipe. The electromagnetic valve 19 is used to control the downward flow of the culture solution in the mixing tank 9 into the drip pipe 15.

[0031] The moving drip structure includes a moving table 23, a moving plate 32, an external motor 8 and two parallel threaded rods 33; the external motor 8 is arranged outside the box body 1, a rotating rod one 22 is provided at the output end of the external motor 8, the rotating rod one 22 penetrates through the box body 1 and is provided with a runner; the two parallel threaded rods 33 penetrate through the moving table 23 and are threadedly connected with the moving table 23, the two ends of the threaded rod 33 are rotatably connected to the box body 1, a runner is provided near one end of the threaded rod 33 close to the external motor 8, and a belt 36 is provided between the runners of the two parallel threaded rods 33 and the runner of the rotating rod one 22 for connection, and the rotating rod one 22 drives the two threaded rods 33 to rotate through the belt 36; a meshing groove 35 is provided at the top of the moving table 23, and a tooth surface is provided in the meshing groove 35; sliding grooves are provided on both sides of the meshing groove 35, and sliding rods 34 are provided in the sliding grooves; the moving plate 32 is located on the top of the moving table 23 and is meshingly and slidably connected with the moving table 23; a clamping structure is provided on the side surface of the moving plate 32, and the clamping structure is used to clamp the drip pipe 15.

[0032] A through meshing port 41 is provided in the middle of the moving plate 32, an internal motor 30 is provided at the top of the moving plate 32, a gear 31 is provided at the output end of the internal motor 30, and the gear 31 is located in the meshing port 41 and meshes with the tooth surface in the meshing groove 35 of the moving table 23; a sliding seat 37 is provided at the bottom of the moving seat, and the sliding seat 37 is slidably connected with the sliding rod 34; a housing 21 is also provided at the top of the moving plate 32 to protect the internal motor 30 and the gear 31; a groove is provided on the side surface of the moving plate 32, and the clamping structure includes a moving clamp body 38, a fixed clamp body 39 and a rotating handle two 40. The moving clamp body 38 and the fixed clamp body 39 are located in the groove, and the rotating handle two 40 is located on the adjacent side surface of the groove and is provided with a rotating shaft passing through the moving plate 32 and threadedly connected with the moving clamp body 38.

[0033] By adopting the above technical solution, the top of the mobile station 23 is provided with an engaging groove 35 and a sliding groove. The moving plate 32 is installed on the top of the mobile station 23 and is engaged and slidably connected with the mobile station 23. Two parallel threaded rods 33 penetrate through the mobile station 23 and are threadedly connected with the mobile station 23. One end of each threaded rod 33 close to the external motor 8 is provided with a runner near the end. The output end of the external motor 8 is provided with a first rotating rod 22. The first rotating rod 22 penetrates through the box body 1 and is provided with a runner. The runners of the first rotating rod 22 and the two parallel threaded rods 33 are respectively connected by a belt 36. During operation, the external motor 8 operates to drive the first rotating rod 22 to rotate, and drives the two parallel threaded rods 33 to rotate through the runners and the belt 36, so that the mobile station 23 moves back and forth left and right on the threaded rods 33, realizing the movement of the position of the drip tube 15 to drip liquid on the culture units of different microtiter plates 12.

[0034] The top of the moving plate 32 is provided with an internal motor 30 to drive a gear 31 to rotate. The gear 31 meshes with the mobile station 23, so that the moving plate 32 can move back and forth on the top of the mobile station 23, changing the position of the drip tube 15. The side surface of the moving plate 32 is provided with a groove and a clamping structure. The movable clamping body 38 and the fixed clamping body 39 are located in the groove. The fixed clamping body 39 is fixedly connected with the moving plate 32. The second rotating handle 40 is located on the side surface adjacent to the groove and is provided with a rotating shaft that penetrates through the moving plate 32 and is threadedly connected with the movable clamping body 38. By rotating the second rotating handle 40, the movable clamping body 38 connected by threads approaches or moves away from the fixed clamping body 39, completing the clamping of the drip tube 15.

[0035] The bottom of the box body 1 is provided with a rotating seat 20, and the placing table 11 is installed on the rotating seat 20; opposite fixing seats 43 are provided on the top of the placing table 11, and a moving groove 44 is provided below the placing table 11 and located below the fixing seats 43; the fixing seats 43 are provided with a rotatable threaded first rotating handle 14, and the first rotating handle 14 is provided with an extrusion member 42, and the extrusion member 42 is located in the moving groove 44. A magnet 13 is provided on the side surface of the placing table 11, and the box door 4 is made of a metal material. The extrusion member 42 includes symmetrically arranged moving push rods 421. The top of the moving push rod 421 is provided with a threaded hole member 422 that is threadedly connected with the first rotating handle 14, and the end of the moving push rod 421 is provided with an extrusion plate 423.

[0036] By adopting the above technical solution, the placement table 11 is installed on the rotating base 20 so that the placement table 11 can rotate, facilitating the picking and placing of the ELISA plate 12. Fixed seats 43 are oppositely arranged on one side of the placement area at the top of the placement table 11, and a first rotating handle 14 rotatably connected to the fixed seat 43 is provided. An extrusion member 42 is threadedly connected to the first rotating handle 14, and a moving groove 44 is provided below the fixed seat 43 to install the extrusion member 42 in the moving groove 44. During use, the ELISA plate 12 is placed in the placement area, and then the first rotating handle 14 is rotated to push the extrusion member 42 out of the moving groove 44, and the extrusion plate 423 squeezes and fixes the side surface of the ELISA plate 12, preventing displacement of the ELISA plate 12 and causing the dropper 15 to be unable to accurately drop liquid, resulting in the failure of mesenchymal stem cell culture. A magnet 13 is provided on the side surface of the placement table 11, and the box door 4 is made of a metal material. Since the placement table 11 is installed on the rotating base 20, external factors may cause the placement table 11 to rotate during the mesenchymal stem cell culture, making the dropper 15 unable to accurately drop the culture medium. By installing a magnet 13 on the side surface of the placement table 11 and making the box door 4 of a metal material, after the box door 4 is closed, the rotation of the placement table 11 can be restricted by the magnet 13 to prevent the placement table 11 from rotating, and at the same time, the box door 4 can be tightly closed.

[0037] Working principle: When the culture device for enhancing the culture of mesenchymal stem cells for treating osteoarthritis of the present invention is in use, the placement table 11 is rotated to place the ELISA plate 12 cultured with mesenchymal stem cells in the placement area of the placement table 11. After rotating the placement table 11 back, the first rotating handle 14 is rotated to push out the extrusion member 42, and the extrusion plate 423 squeezes and fixes the side surface of the ELISA plate 12 to prevent it from moving. The box door 4 is closed, and the raw materials for preparing the culture medium are added into the mixing box 9 through the first feeding port and the second feeding port. The stirring motor 24 is started to stir evenly. The dropping amount of the culture medium per time and the time interval for the next dropping are set through the display and control device 5 on the box door 4. The control box 16 controls the external motor 8 to move the moving table 23 left and right to change the position of the dropper 15, controls the internal motor to move the moving plate 32 back and forth on the moving table 23 to change the position of the dropper 15, and changes the position of the clamped dropper 15 through the moving dropping structure to accurately drop the culture medium into the culture unit of the ELISA plate 12. The micro flowmeter 47 can accurately monitor and control the amount of the nutrient solution dropped each time, enabling the set amount of the culture medium to be dropped regularly and quantitatively.

[0038] This specific embodiment is only an explanation of the present invention and is not a limitation of the present invention. Those skilled in the art can make modifications to this embodiment without creative contributions according to needs after reading this specification, but as long as it is within the scope of the claims of the present invention, it is protected by the patent law.

Claims

1. A culture device for enhancing mesenchymal stem cells for treating osteoarthritis, characterized by: The invention comprises a box body (1), the top of the box body (1) is open and provided with a box cover (3) that covers the box body, and the side of the box body (1) is provided with a box door (4) that opens and closes; the interior of the box body (1) is provided with a mixing box (9), a movable dripping structure and a placement table (11) in order from top to bottom; the box cover (3) is provided with a first discharge port and a second discharge port that penetrate through the box, and the mixing box (9) is connected to the first discharge port and the second discharge port; the bottom of the mixing box (9) is provided with a discharge pipe, and the discharge pipe is provided with a An electromagnetic valve (19); the mobile dripping structure is fixedly arranged inside the box body (1), the mobile dripping structure is provided with a dripping tube (15), the top of the dripping tube (15) is provided with a connecting tube (17) connected to the discharge tube, and the bottom of the dripping tube (15) is provided with a micro flow meter (47); the placement table (11) is provided with a control box (16) and a placement area, and the placement area is used to place the ELISA plate (12); the front of the box door (4) is provided with a display and control device (5).

2. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 1, characterized in that: The top of the mixing box (9) is provided with a first feed port (27), a second feed port (28) and a sunken installation groove (29), wherein the first feed port (27) and the second feed port (28) are respectively connected to the first discharge port and the second discharge port of the box cover (3); a stirring motor (24) is arranged in the installation groove (29), and a second rotating rod (25) is arranged at the output end of the stirring motor (24), wherein the second rotating rod (25) passes through the top of the mixing box (9), and the second rotating rod (25) is provided with a stirring rod (26).

3. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 2, characterized in that: A mounting ring (10) is provided inside the box body (1), a plurality of support blocks (18) are provided on the same horizontal plane of the inner wall of the box body (1), the mounting ring (10) is fixedly arranged on the support blocks (18), and the mixing box (9) is detachably mounted on the mounting ring (10).

4. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 1, characterized in that: The mobile dripping structure comprises a mobile platform (23), a mobile plate (32), an external motor (8) and two parallel threaded rods (33); the external motor (8) is arranged on the outside of the box (1); a rotating rod (22) is provided at the output end of the external motor (8); the rotating rod (22) passes through the box (1) and is provided with a rotating wheel; the two parallel threaded rods (33) pass through the mobile platform (23) and are threadedly connected to the mobile platform (23); both ends of the threaded rod (33) are rotatably connected to the box (1); one end of the threaded rod (33) close to the external motor (8) is provided with a rotating wheel; the two parallel threaded rods (33) pass through the mobile platform (23) and are threadedly connected to the mobile platform (23); both ends of the threaded rod (33) are rotatably connected to the box (1); one end of the threaded rod (33) close to the external motor (8) is provided with a rotating wheel; A belt (36) is provided between the rotating wheel of the threaded rod (33) and the rotating wheel of the rotating rod (22), and the rotating rod (22) drives the two threaded rods (33) to rotate through the belt (36); a meshing groove (35) is provided on the top of the moving platform (23), and a tooth surface is provided in the meshing groove (35); sliding grooves are provided on both sides of the meshing groove (35), and a sliding bar (34) is provided in the sliding groove; the moving plate (32) is located on the top of the moving platform (23) and is meshed and slidably connected with the moving platform (23); a clamping structure is provided on the side of the moving plate (32), and the clamping structure is used to clamp the drip tube (15).

5. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 4, characterized in that: A through meshing opening (41) is provided in the middle of the movable plate (32); a built-in motor (30) is provided on the top of the movable plate (32); a gear (31) is provided at the output end of the built-in motor (30); the gear (31) is located in the meshing opening (41) and meshes with the tooth surface in the meshing groove (35) of the movable platform (23); a sliding seat (37) is provided at the bottom of the movable seat; the sliding seat (37) is slidably connected to the slide bar (34); a shell (21) is also provided on the top of the movable plate (32) to protect the built-in motor (30) and the gear (31); a groove is provided on the side of the movable plate (32); the clamping structure comprises a movable clamping body (38), a fixed clamping body (39) and a second rotating handle (40); the movable clamping body (38) and the fixed clamping body (39) are located in the groove; the second rotating handle (40) is located on the side adjacent to the groove and is provided with a rotating shaft that passes through the movable plate (32) and is threadedly connected to the movable clamping body (38).

6. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 1, characterized in that: A rotating seat (20) is provided at the bottom of the box body (1), and the placement platform (11) is installed on the rotating seat (20); a fixed seat (43) is provided at the top of the placement platform (11), and a movable groove (44) is provided below the fixed seat (43) on the placement platform (11); the fixed seat (43) is provided with a rotating threaded handle (14), and the rotating handle (14) is provided with an extrusion component (42), and the extrusion component (42) is located in the movable groove (44).

7. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 6, characterized in that: A magnet (13) is provided on the side of the placement table (11), and the box door (4) is made of metal material.

8. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 6, characterized in that: The extrusion member (42) comprises a symmetrically arranged moving push rod (421), the top of which is provided with a threaded hole member (422) threadedly connected to a turning handle (14), and the end of the moving push rod (421) is provided with an extrusion plate (423).

9. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 1, characterized in that: The side of the box body (1) is provided with ear plates (2) near the top, and the bottom of the box body (1) is provided with supporting feet (46).

10. The culture device for enhancing mesenchymal stem cells for treating osteoarthritis according to claim 1, characterized in that: A handle (45) is provided on the top of the box cover (3), and the first discharge port and the second discharge port are respectively provided with a cover body 1 (6) and a cover body 2 (7) for covering.

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