Intelligent regulation and control cell adherence promotion device

Abstract

The invention discloses an intelligent regulation and control cell adherence promotion device which comprises a cell culture box and a culture dish, an accessory box is mounted on one side of the cell culture box, a box door is rotatably mounted on the front side of the cell culture box, a placement table is mounted on the bottom surface in the cell culture box, and an electric push rod is mounted at the top of the cell culture box; an electric push rod is arranged in the accessory box, an output end of the electric push rod slidably extends into the cell culture box, a front regulation and control assembly is arranged at the output end of the electric push rod, and a first pump box and a buffer solution tank are arranged in the accessory box. A buffer solution supplementing gun head, a solution supply pump in a first pump box and a corresponding intelligent flow valve can be automatically triggered in time, supplementing liquid is added into a corresponding culture dish, the interior of the culture dish reaches the needed PH value, and therefore the PH value in the culture dish is automatically regulated in real time, and cell attachment is promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and particularly to an intelligent regulation cell adhesion promotion device. Background Art

[0002] Promoting cell adhesion is an important link in the process of cell culture, which has a crucial impact on cell growth, proliferation and differentiation.

[0003] After retrieval, as disclosed in a cell adhesion promoting electromagnetic device with Chinese patent number CN 217868922 U, the electromagnetic device includes a culture dish with cell culture medium inside; the cell membranes on the surface of the cells in the culture medium carry charges; the culture dish is placed at the central axis of the electromagnetic induction coil; the magnetic field direction at the central axis of the electromagnetic induction coil is vertical; the magnetic field of the electromagnetic induction coil applies a downward electromagnetic force to the cells in the culture medium to accelerate the sedimentation of the cells to the bottom of the culture dish; the utility model can accelerate the cell adhesion speed in the culture dish.

[0004] Again, as disclosed in a device for promoting cell adhesion and growth and its use method with Chinese patent number CN 113699045 A, the device includes: a cell culture equipment, a tray supporting the cell culture equipment, an electret located between the cell culture equipment and the tray, and a sealing ring for sealing the electret between the cell culture equipment and the tray; the bottom wall thickness of the cell culture equipment is 0.1 to 0.5 mm. The present invention also provides a use method of the device for promoting cell adhesion and growth. The device for promoting cell adhesion and growth of the present invention has a simple structure, high production efficiency, convenient use, does not change the components of the culture medium, has little influence on the physiological state of cells, and has broad application prospects.

[0005] However, in the process of culturing cells with the above two cell adhesion promotion devices, it is not convenient to monitor the pH value of the culture medium in real time, and at the same time, when the pH value of the culture medium changes, it is not convenient to monitor and automatically adjust the pH value of the culture medium in real time, thus affecting the cell adhesion success rate.

[0006] Therefore, it is very necessary to invent an intelligent regulation cell adhesion promotion device. Summary of the Invention

[0007] The purpose of the present invention is to provide an intelligent regulation cell adhesion promotion device to solve the problem that in the process of culturing cells for adhesion, it is not convenient to monitor the pH value of the culture medium in real time, and at the same time, when the pH value of the culture medium changes, it is not convenient to monitor and automatically adjust the pH value of the culture medium in real time, thus affecting the cell adhesion success rate.

[0008] To achieve the above object, the present invention provides the following technical solution: An intelligent regulation cell adhesion promotion device: including a cell culture incubator and a culture dish, wherein: A fitting box is fixedly installed on one side of the cell culture incubator, a box door is rotatably installed on the front side of the cell culture incubator, a placement table for placing the culture dish is detachably and fixedly installed on the bottom surface inside the cell culture incubator, an electric push rod is detachably and fixedly installed on the top of the cell culture incubator, the output end of the electric push rod slides into the interior of the cell culture incubator, a pre-regulation component is detachably and fixedly installed at the output end of the electric push rod, the pre-regulation component is located above the placement table, the culture dishes are evenly placed on the placement table, a pump box one and a buffer solution tank are detachably and fixedly installed inside the fitting box, and the pump box one is located above the buffer solution tank;

[0009] Preferably, the pre-regulation component includes a lower support disc, a plurality of pH detection probes and a plurality of buffer solution replenishment gun heads are evenly and fixedly installed on the lower support disc, the number of pH detection probes is the same as the number of buffer solution replenishment gun heads, the pH detection probes are outside the buffer solution replenishment gun heads, the heads of the pH detection probes and the buffer solution replenishment gun heads both extend from the bottom surface of the lower support disc, and the center of the upper surface of the lower support disc is detachably and fixedly connected to the output end of the electric push rod;

[0010] Preferably, the culture dish includes a bottom shell and an upper cover, and the bottom shell and the upper cover are connected by plugging;

[0011] Preferably, an integrated positioning table is arranged on one side inside the bottom shell, a monitoring hole is opened from top to bottom in the positioning table, a liquid inlet hole is opened on one side of the bottom of the positioning table, and the liquid inlet hole is communicated with the bottom shell and the monitoring hole;

[0012] Preferably, an inlet corresponding to the monitoring hole is opened on one side of the surface of the upper cover, a liquid supply hole is opened at the center of the surface of the upper cover, an atomizing nozzle is fixedly installed at the center of the bottom surface of the upper cover, the liquid inlet of the atomizing nozzle is communicated with the liquid supply hole, the pH detection probe is inserted into the corresponding monitoring hole through the inlet, and the buffer solution replenishment gun head is inserted into the corresponding liquid supply hole; A plugging device for plugging the inlet and the liquid supply hole is rotatably installed outside the upper surface of the upper cover;

[0013] Preferably, the liquid inlet end of the liquid supply pump inside the pump box one is fixedly communicated with the buffer solution tank, and the liquid outlet end of the liquid supply pump inside the pump box one is fixedly communicated with each buffer solution replenishment gun head through an intelligent flow valve;

[0014] Preferably, a control box is fixedly installed on the fitting box;

[0015] Preferably, the cell incubator, the electric push rod, the pH detection probe, the buffer solution replenishing nozzle, the liquid supply pump and the intelligent flow valve are connected to the intelligent control system inside the control box.

[0016] Preferably, a second pump box, a third pump box, a fourth pump box, a carbon dioxide tank, an oxygen tank and a disinfectant solution tank are fixedly installed inside the accessory box. The second pump box is above the carbon dioxide tank, the third pump box is above the oxygen tank, and the fourth pump box is above the disinfectant solution tank. The liquid inlet end of the liquid supply pump inside the second pump box is fixedly communicated with the carbon dioxide tank. The liquid inlet end of the liquid supply pump inside the third pump box is fixedly communicated with the oxygen tank. The liquid inlet end of the liquid supply pump inside the fourth pump box is fixedly communicated with the disinfectant solution tank. The liquid outlet ends of the liquid supply pumps inside the second pump box and the third pump box are both communicated with the inside of the cell incubator. The liquid outlet end of the liquid supply pump inside the fourth pump box is communicated with the placement table.

[0017] Preferably, carbon dioxide detection sensors, oxygen sensors, carbon dioxide replenishing nozzles and oxygen replenishing nozzles are uniformly and fixedly installed on one side inside the cell incubator. An exhaust channel is arranged on one side of the cell incubator, and an exhaust fan and an electromagnetic valve are fixedly installed in the exhaust channel. The carbon dioxide replenishing nozzle is fixedly communicated with the liquid outlet end of the liquid supply pump inside the second pump box through a pipeline and an intelligent flow valve. The oxygen replenishing nozzle is fixedly communicated with the liquid outlet end of the liquid supply pump inside the third pump box through a pipeline and an intelligent flow valve. The carbon dioxide detection sensors, the oxygen sensors, the exhaust fan and the electromagnetic valve are connected to the intelligent control system inside the control box.

[0018] Preferably, a plurality of placement cavities for placing culture dishes are uniformly formed on the upper surface of the placement table. A protrusion is arranged on one side inside the placement cavity. A disinfection cavity is formed in the placement cavity of the placement table. The number of the disinfection cavities is the same as that of the pH detection probes, and the disinfection cavity is fixedly communicated with the liquid outlet end of the liquid supply pump inside the fourth pump box through a pipeline. The culture dish is clamped with the protrusion. A drain pipe is arranged on one side of the placement table and is communicated with each disinfection cavity. An electromagnetic valve II is fixedly installed on the drain pipe. The electromagnetic valve II is connected to the intelligent control system inside the control box.

[0019] Preferably, a hollow wire routing plate is fixedly installed above the lower support plate. One side of the hollow wire routing plate close to the accessory box is uniformly and fixedly installed with a first wire pipe and a second wire pipe. The probe control wire of the pH detection probe passes through the hollow wire routing plate and the first wire pipe and is connected to the intelligent control system inside the control box. The gun head control wire of the buffer solution replenishing nozzle passes through the hollow wire routing plate and the second wire pipe and is connected to the intelligent control system inside the control box. The probe control wire of the pH detection probe and the gun head control wire of the buffer solution replenishing nozzle both adopt spiral elastic wires.

[0020] Preferably, a positioning groove is formed on one side below the outside of the bottom case, and the positioning groove is snap-fitted with the corresponding protrusion.

[0021] Preferably, two limiting holes are formed on the surface of the positioning platform, and the two limiting holes are evenly distributed on both sides of the monitoring hole. The limiting holes are connected to the upper cover in a plug-and-play manner.

[0022] Preferably, two positioning posts are fixedly installed on one side of the top surface inside the upper cover, and the two positioning posts are evenly distributed on both sides of the inlet. The positioning posts are connected to the corresponding limiting holes in a plug-and-play manner.

[0023] Preferably, the plugging device includes an elastic sheet and a rotating shaft. One end of the elastic sheet is fixedly installed with a plugging cap head one for plugging the liquid supply hole, and the other end of the elastic sheet is fixedly installed with a plugging cap head two for plugging the inlet; the center of the surface of the elastic sheet is fixedly connected to the top end of the rotating shaft, and the bottom end of the rotating shaft is rotatably connected to the upper surface of the upper cover. The rotating shaft is located between the inlet and the liquid supply hole; the plugging cap head one is connected to the liquid supply hole in a plug-and-play manner; the plugging cap head two is connected to the inlet in a plug-and-play manner.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0025] Through the overall setting of the present invention, when culturing cells adherently, the pH value inside the culture dish can be monitored in real time by the pH detection probe. Once it is found that the pH value inside the culture dish changes, such as when the pH value inside the culture dish does not reach the required value, the buffer solution replenishing gun head, the liquid supply pump inside the pump box one, and the corresponding intelligent flow valve can be automatically triggered in time to add replenishing liquid into the corresponding culture dish, so that the pH value inside the culture dish reaches the required value, thereby realizing real-time automatic regulation of the pH value inside the culture dish and promoting cell adhesion. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the separation structure of the pre-regulation component and the culture dish of the present invention.

[0027] Figure 2 It is a schematic diagram of the contact structure of the pre-regulation component and the culture dish of the present invention.

[0028] Figure 3 It is a schematic diagram of the placement platform structure of the present invention.

[0029] Figure 4 It is a schematic diagram of the pre-regulation component structure of the present invention.

[0030] Figure 5 It is a schematic diagram of the plugging structure of the plugging device of the present invention.

[0031] Figure 6 It is a schematic diagram of the opened structure of the plugging device of the present invention.

[0032] Figure 7 It is a schematic diagram of the explosion structure of the petri dish of the present invention.

[0033] Figure 8 It is a schematic diagram of the partial enlarged structure at position A of the present invention.

[0034] Figure 9 It is a schematic diagram of the partial enlarged structure at position B of the present invention.

[0035] In the figure:

[0036] Cell incubator 1, accessory box 2, box door 3, placement table 4, placement cavity 41, protrusion 42, disinfection cavity 43, electric push rod 5, pre-regulation component 6, lower support disk 61, PH value detection probe 62, buffer solution replenishment gun head 63, hollow wire tray 64, wire pipe one 65, wire pipe two 66, probe control wire 67, gun head control wire 68, petri dish 7, bottom shell 71, upper cover 72, positioning table 73, monitoring hole 74, limiting hole 75, liquid inlet hole 76, positioning groove 77, entrance 78, liquid supply hole 79, positioning column 70, carbon dioxide detection sensor 8, oxygen sensor 9, carbon dioxide replenishment spray head 10, oxygen replenishment spray head 11, external discharge channel 12, pump box one 13, pump box two 14, pump box three 15, pump box four 16, buffer solution tank 17, carbon dioxide tank 18, oxygen tank 19, disinfectant solution tank 20, control box 21, elastic sheet 01, plugging cap head one 02, plugging cap head two 03, rotating shaft 04, atomizing spray head 05. Specific embodiments

[0037] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0038] Example:

[0039] As shown in the appendix Figures 1-9 shown

[0040] An intelligent regulation cell adhesion promotion device provided by the present invention includes a cell incubator 1 and a culture dish 7, wherein: a fitting box 2 is fixedly installed on one side of the cell incubator 1, and a box door 3 is rotatably installed on the front side of the cell incubator 1, so that during the adherent culture of cells, the cell incubator 1 can be selectively sealed and aseptically cultured through the box door 3 alone. A placement table 4 for placing the culture dish 7 is detachably and fixedly installed on the bottom surface inside the cell incubator 1, so as to limit the culture dish 7 and ensure the stability of the contact and separation between the culture dish 7 and the pre-regulation component 6. An electric push rod 5 is detachably and fixedly installed on the top of the cell incubator 1, so as to drive the pre-regulation component 6 to contact or separate from the culture medium inside the culture dish 7 through the electric push rod 5. The output end of the electric push rod 5 slides into the cell incubator 1, and the output end of the electric push rod 5 is detachably and fixedly installed with a pre-regulation component 6, so as to monitor the pH value inside the culture dish 7 by the pre-regulation component 6. The pre-regulation component 6 is located above the placement table 4, and the culture dishes 7 are evenly placed on the placement table 4. A pump box one 13 and a buffer tank 17 are detachably and fixedly installed inside the fitting box 2. The pump box one 13 is located above the buffer tank 17, so as to provide a buffer solution for adjusting the pH value to the culture dish 7 and promote cell adhesion, thereby ensuring the success rate of cell adhesion;

[0041] Relevant parameters of the cell incubator 1:

[0042] Adopt electronically controlled APT.line(R) inner cavity preheating technology and high-efficiency refrigeration system;

[0043] Temperature range: -5°C to 100°C (when room temperature ≤ 20°C);

[0044] Digital temperature setting, with an accuracy of 0.1°C; MP program controller, having 2 programs (each with 10 segments) or can be switched to 1 program (20 segments); the interval time of each segment of a single program can be adjusted to a maximum of 99:59 hours or 999:59 hours;

[0045] Weekly program timing function;

[0046] Independent adjustable temperature safety device, class 3.1 (DIN 12880), with visual and audible alarms;

[0047] The box door 3 is made of a glass door;

[0048] In this embodiment, before regulation, the component 6 includes a lower support plate 61 to support the pH detection probe 62 and the buffer solution replenishing nozzle 63, and ensure the stability of the pH detection probe 62 and the buffer solution replenishing nozzle 63 during operation. A number of pH detection probes 62 and a number of buffer solution replenishing nozzles 63 are evenly and fixedly installed on the lower support plate 61, so as to detect the pH value inside the culture dish 7 in real time through the pH detection probe 62, and according to the monitoring data of the pH detection probe 62, spray the corresponding replenishing solution into the culture dish 7 through the buffer solution replenishing nozzle 63. The number of pH detection probes 62 is the same as the number of buffer solution replenishing nozzles 63. The pH detection probe 62 is on the outside of the buffer solution replenishing nozzle 63. The heads of the pH detection probe 62 and the buffer solution replenishing nozzle 63 both extend from the bottom surface of the lower support plate 61. It should be noted that the length of the pH detection probe 62 is greater than the length of the buffer solution replenishing nozzle 63. When the electric push rod 5 is fully extended, the pH detection probe 62 will enter the corresponding monitoring hole 74, and the buffer solution replenishing nozzle 63 will enter the corresponding liquid supply hole 79. The center of the upper surface of the lower support plate 61 is detachably and fixedly connected to the output end of the electric push rod 5;

[0049] Relevant parameters of the pH detection probe 62:

[0050] Measurement range: pH 0 to 14.00, so that the instrument can measure a wide range from acidic to alkaline.

[0051] Resolution: 0.01pH, so that the instrument can accurately measure small changes in pH value.

[0052] Accuracy: 0.1PH, so that the instrument measures more accurately.

[0053] Temperature compensation range: 0 - 99°C automatic compensation, so that the instrument can automatically adjust the measurement result according to the change of temperature to ensure accuracy.

[0054] Power supply: It can be a 9V power adapter or a built-in battery, or AC220V or DC24V, depending on the specific model and design of the instrument.

[0055] In addition, there are some other important parameters and technical features, such as:

[0056] Input impedance: ≥1×10^12Ω, indicating that the high input impedance of the instrument can reduce measurement errors.

[0057] Stability: ≤0.01PH / 3h, indicating that the change in pH value of the instrument within 3 hours does not exceed 0.01PH, ensuring the stability of measurement.

[0058] Ambient temperature and humidity: It is required to be used within a certain temperature and humidity range to ensure the normal operation of the instrument and measurement accuracy. For example, the relative humidity should not be greater than 85%, and the ambient temperature is between 5 - 60 °C.

[0059] Degree of protection: Such as IP65 and IP68, so that the instrument has a certain ability to prevent water and dust, thus adapting to different working environments;

[0060] In this embodiment, the culture dish 7 includes a bottom shell 71 and an upper cover 72. The bottom shell 71 and the upper cover 72 are connected by plugging. The culture dish 7 is circular as a whole and is made of plastic or glass material;

[0061] Cleaning steps for glass culture dish 7:

[0062] 1. Soaking: New or used glassware should be soaked in clean water first to soften and dissolve the attachments. New glassware should be simply scrubbed with tap water before use, and then soaked in 5% hydrochloric acid overnight; Used glassware often adheres to a large amount of protein and grease, which is not easy to scrub off after drying, so it should be immediately immersed in clean water for scrubbing after use.

[0063] 2. Scrubbing: Put the soaked glassware into the detergent water and scrub it repeatedly with a soft brush. Do not leave any dead corners and prevent damage to the smoothness of the surface of the ware. Wash the scrubbed glassware clean and dry it for acid immersion.

[0064] 3. Acid immersion: Acid immersion is to soak the above-mentioned ware into the cleaning solution, also known as acid solution, to remove the possible residual substances on the surface of the ware through the strong oxidation of the acid solution. The acid immersion should not be less than six hours, usually overnight or longer. Be careful when putting in and taking out the ware.

[0065] 4. Rinsing: The ware after scrubbing and acid immersion must be rinsed thoroughly with water. Whether the ware after acid immersion is rinsed clean directly affects the success or failure of cell culture. Manually wash the ware after acid immersion, and each piece of ware should be repeatedly "filled with water - emptied" at least 15 times, and finally soaked and washed with double-distilled water 2 - 3 times, and then dried or baked and packaged for standby.

[0066] In this embodiment, an integrated positioning platform 73 is provided on one side inside the bottom shell 71. The positioning platform 73 is provided with a monitoring hole 74 from top to bottom, so as to provide a separate insertion space for the pH detection probe 62, preventing the pH detection probe 62 from damaging the cells inside the bottom shell 71 after being inserted into the bottom shell 71. A liquid inlet hole 76 is provided on one side of the bottom of the positioning platform 73. The liquid inlet hole 76 is communicated with the bottom shell 71 and the monitoring hole 74, so that the culture medium inside the bottom shell 71 can enter the monitoring hole 74 and contact the pH detection probe 62;

[0067] In this embodiment, an inlet 78 corresponding to the monitoring hole 74 is provided on one side of the upper cover 72. After the upper cover 72 is covered on the bottom case 71, it is convenient for the pH detection probe 62 to be inserted into the monitoring hole 74. A liquid supply hole 79 is provided at the center of the upper surface of the upper cover 72 to communicate with the buffer solution replenishing nozzle 63, so as to provide buffer solution for the atomizing nozzle 05 through the buffer solution replenishing nozzle 63. The atomizing nozzle 05 is fixedly installed at the center of the bottom surface of the upper cover 72, so that the buffer solution is evenly sprayed on the culture medium inside the bottom case 71 through the atomizing nozzle 05. The liquid inlet of the atomizing nozzle 05 communicates with the liquid supply hole 79. The pH detection probe 62 is inserted into the corresponding monitoring hole 74 through the inlet 78, and the buffer solution replenishing nozzle 63 is inserted into the corresponding liquid supply hole 79. A plugging device for plugging the inlet 78 and the liquid supply hole 79 is rotatably installed outside the upper surface of the upper cover 72 to plug or open the inlet 78 and the liquid supply hole 79.

[0068] In this embodiment, the liquid inlet end of the liquid supply pump inside the first pump box 13 is fixedly communicated with the buffer solution tank 17, and the liquid outlet end of the liquid supply pump inside the first pump box 13 is fixedly communicated with each buffer solution replenishing nozzle 63 through an intelligent flow valve, so as to convey the required buffer solution from the buffer solution tank 17 to the buffer solution replenishing nozzle 63 through the liquid supply pump and the flow valve.

[0069] In this embodiment, a control box 21 is fixedly installed on the accessory box 2.

[0070] In this embodiment, the cell culture box 1, the electric push rod 5, the pH detection probe 62 and the buffer solution replenishing nozzle 63, the liquid supply pump and the intelligent flow valve are connected to the intelligent control system inside the control box 21.

[0071] In this embodiment, a second pump box 14, a third pump box 15, a fourth pump box 16, a carbon dioxide tank 18, an oxygen tank 19 and a disinfectant solution tank 20 are also fixedly installed inside the accessory box 2. The second pump box 14 is above the carbon dioxide tank 18, the third pump box 15 is above the oxygen tank 19, and the fourth pump box 16 is above the disinfectant solution tank 20. The liquid inlet end of the liquid supply pump inside the second pump box 14 is fixedly communicated with the carbon dioxide tank 18. The liquid inlet end of the liquid supply pump inside the third pump box 15 is fixedly communicated with the oxygen tank 19. The liquid inlet end of the liquid supply pump inside the fourth pump box 16 is fixedly communicated with the disinfectant solution tank 20. The liquid outlet ends of the liquid supply pumps inside the second pump box 14 and the third pump box 15 are both communicated with the inside of the cell culture box 1 to adjust the oxygen content and carbon dioxide content inside the cell culture box 1. The liquid outlet end of the liquid supply pump inside the fourth pump box 16 is communicated with the placing table 4 to provide the required disinfectant solution for the placing table 4.

[0072] In this embodiment, a carbon dioxide detection sensor 8, an oxygen sensor 9, a carbon dioxide replenishment nozzle 10, and an oxygen replenishment nozzle 11 are uniformly and fixedly installed on one side inside the cell incubator 1, so as to detect the oxygen content and carbon dioxide content inside the cell incubator 1 through the carbon dioxide detection sensor 8 and the oxygen sensor 9. When the carbon dioxide and oxygen inside the cell incubator 1 are lower than the required dosage, the required carbon dioxide and oxygen can be replenished into the cell incubator 1 through the carbon dioxide replenishment nozzle 10 and the oxygen replenishment nozzle 11. And an exhaust channel 12 is provided on one side of the cell incubator 1. An exhaust fan and an electromagnetic valve are fixedly installed in the exhaust channel 12. When the carbon dioxide and oxygen inside the cell incubator 1 are greater than the required dosage, the excess carbon dioxide and oxygen inside the cell incubator 1 can be discharged through the exhaust fan; A pipeline and an intelligent flow valve are fixedly connected between the carbon dioxide replenishment nozzle 10 and the liquid outlet end of the liquid supply pump inside the pump box two 14; A pipeline and an intelligent flow valve are fixedly connected between the oxygen replenishment nozzle 11 and the liquid outlet end of the liquid supply pump inside the pump box three 15; The carbon dioxide detection sensor 8, the oxygen sensor 9, the exhaust fan, and the electromagnetic valve are connected to the intelligent control system inside the control box 21.

[0073] The fan speed of the exhaust fan is adjustable (0 to 100%);

[0074] In this embodiment, a number of placement cavities 41 for placing the culture dishes 7 are uniformly formed on the upper surface of the placement table 4, so as to provide a separate placement space for the culture dishes 7. And a protrusion 42 is provided on one side inside the placement cavity 41, so as to limit the culture dish 7 and ensure the stability of the culture dish 7 in the placement cavity 41, so that the culture dish 7 can accurately dock with the pre-regulation component 6. A disinfection cavity 43 is formed in the placement cavity 41 of the placement table 4, so as to convey the disinfectant inside the disinfectant tank 20 to the disinfection cavity 43 through the liquid supply pump inside the pump box four 16 to disinfect the pH detection probe 62; The number of the disinfection cavities 43 is the same as the number of the pH detection probes 62, and the disinfection cavities 43 are fixedly connected to the liquid outlet end of the liquid supply pump inside the pump box four 16 through pipelines; The culture dish 7 is snap-connected with the protrusion 42; A drain pipe is provided on one side of the placement table 4, and the drain pipe is communicated with each disinfection cavity 43. An electromagnetic valve two is fixedly installed on the drain pipe, so as to discharge the used disinfectant inside the disinfection cavity 43; The electromagnetic valve two is connected to the intelligent control system inside the control box 21.

[0075] In this embodiment, a hollow wire routing plate 64 is fixedly installed above the lower support plate 61. On one side of the hollow wire routing plate 64 close to the accessory box 2 above, a first wire pipe 65 and a second wire pipe 66 are evenly and fixedly installed, so as to conduct centralized wire routing for the probe control line 68 of the buffer solution replenishing nozzle 63, making it more beautiful and tidy and preventing entanglement. The probe control line 67 of the pH detection probe 62 passes through the hollow wire routing plate 64 and the first wire pipe 65 and is connected to the intelligent control system inside the control box 21. The probe control line 68 of the buffer solution replenishing nozzle 63 passes through the hollow wire routing plate 64 and the second wire pipe 66 and is connected to the intelligent control system inside the control box 21. The probe control line 67 of the pH detection probe 62 and the probe control line 68 of the buffer solution replenishing nozzle 63 both adopt spiral elastic wires, so as to be able to stretch and contract when the front component 6 moves up and down before regulation, preventing breakage.

[0076] In this embodiment, a positioning groove 77 is provided on one side below the outside of the bottom shell 71, and the positioning groove 77 is clamped with the corresponding protrusion 42, so as to ensure the stability of the bottom shell 71 placed in the placement cavity 41 and ensure that the monitoring hole 74 can correspond to the corresponding pH detection probe 62.

[0077] In this embodiment, two limiting holes 75 are provided on the surface of the positioning table 73, and the two limiting holes 75 are evenly distributed on both sides of the monitoring hole 74. The limiting holes 75 are connected to the upper cover 72 in a plug-and-play manner, so as to limit the connection method between the upper cover 72 and the bottom shell 71, prevent the upper cover 72 from rotating, and enable the pH detection probe 62 to be inserted into the corresponding monitoring hole 74 through the inlet 78.

[0078] In this embodiment, two positioning posts 70 are fixedly installed on one side of the top surface inside the upper cover 72, and the two positioning posts 70 are evenly distributed on both sides of the inlet 78. The positioning posts 70 are connected to the corresponding limiting holes 75 in a plug-and-play manner, so as to perform positioning connection between the upper cover 72 and the bottom shell 71 and ensure the stability between the upper cover 72 and the bottom shell 71.

[0079] In this embodiment, the plugging device includes an elastic sheet 01 and a rotating shaft 04. One end of the elastic sheet 01 is fixedly installed with a first plugging cap head 02 for plugging the liquid supply hole 79, and the other end of the elastic sheet 01 is fixedly installed with a second plugging cap head 03 for plugging the inlet 78. Through the arrangement of the elastic sheet 01, the first plugging cap head 02 and the second plugging cap head 03 can be disassembled and assembled with the inlet 78 and the liquid supply hole 79; the center of the surface of the elastic sheet 01 is fixedly connected to the top end of the rotating shaft 04, and the bottom end of the rotating shaft 04 is rotatably connected to the upper surface of the upper cover 72. Through the arrangement of the rotating shaft 04, the loss of the first plugging cap head 02 and the second plugging cap head 03 can be prevented. At the same time, after the first plugging cap head 02 and the second plugging cap head 03 are opened, the first plugging cap head 02 and the second plugging cap head 03 can be staggered from the inlet 78 and the liquid supply hole 79 to prevent interference when the pH detection probe 62 and the buffer solution replenishing gun head 63 enter the inlet 78 and the liquid supply hole 79. The rotating shaft 04 is located between the inlet 78 and the liquid supply hole 79; the first plugging cap head 02 is connected to the liquid supply hole 79 in a pluggable manner; the second plugging cap head 03 is connected to the inlet 78 in a pluggable manner.

[0080] When performing adherent culture on cells, place the cells in the corresponding culture dish 7, and open the first plugging cap head 02 and the second plugging cap head 03 to stagger the first plugging cap head 02 and the second plugging cap head 03 from the inlet 78 and the liquid supply hole 79;

[0081] Then, place the culture dish 7 containing the cells in the corresponding placement cavity 41 in sequence, and close the box door 3; drive the front regulating assembly 6 to descend through the electric push rod 5. When the electric push rod 5 is fully extended, the pH detection probe 62 on the front regulating assembly 6 will enter the monitoring hole 74 through the inlet 78, and the buffer solution replenishing gun head 63 will enter the corresponding liquid supply hole 79;

[0082] So that the pH detection probe 62 can monitor the pH value inside the culture dish 7 in real time. When the pH value inside the culture dish 7 is lower than the required pH value, the corresponding replenishing solution is sprayed into the culture dish 7 through the buffer solution replenishing gun head 63 and the atomizing nozzle 05, so that the pH value inside the culture dish 7 reaches the required pH value, thereby promoting the cells to adhere inside the culture dish 7;

[0083] When the pH value inside the culture dish 7 is greater than the required pH value, the excess carbon dioxide and oxygen inside the cell culture box 1 can be discharged through the exhaust fan. At the same time, the carbon dioxide detection sensor 8 and the oxygen sensor 9 can detect the oxygen content and carbon dioxide content inside the cell culture box 1. When the carbon dioxide and oxygen inside the cell culture box 1 are lower than the required dosage, the required carbon dioxide and oxygen can be replenished into the cell culture box 1 through the carbon dioxide replenishing nozzle 10 and the oxygen replenishing nozzle 11.

[0084] All kinds of components used in this application document are standard parts. The specific connection methods of each part adopt conventional means such as threads, bolts, and nesting that are mature in the prior art. Each structure adopts conventional materials in the prior art, and no specific description will be made here.

[0085] In summary, for this intelligent cell adhesion promotion device with regulation, through the overall setting, it can monitor the pH value inside the culture dish in real time through the pH detection probe. Once it is found that the pH value inside the culture dish is relatively low, it can automatically trigger the buffer solution replenishment gun head, the liquid supply pump inside the first pump box, and the corresponding intelligent flow valve in a timely manner to add replenishing liquid to the corresponding culture dish, so that the pH value inside the culture dish reaches the required value, thereby realizing real-time automatic regulation of the pH value inside the culture dish and promoting cell adhesion.

[0086] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intelligent device for promoting cell adhesion, characterized in that: The invention comprises a cell culture box (1) and a culture dish (7), wherein: an accessory box (2) is fixedly installed on one side of the cell culture box (1); a box door (3) is rotatably installed on the front side of the cell culture box (1); a placement table (4) for placing the culture dish (7) is detachably fixedly installed on the bottom surface of the cell culture box (1); an electric push rod (5) is detachably fixedly installed on the top of the cell culture box (1); the output end of the electric push rod (5) slides into the cell culture box (1); a front regulating component (6) is detachably fixedly installed on the output end of the electric push rod (5); the front regulating component (6) is located above the placement table (4); the culture dishes (7) are evenly placed on the placement table (4); a pump box (13) and a buffer tank (17) are detachably fixedly installed inside the accessory box (2); the pump box (13) is located above the buffer tank (17); The front-regulating component (6) comprises a lower supporting plate (61), on which a plurality of pH value detection probes (62) and a plurality of buffer replenishing gun heads (63) are evenly and fixedly mounted, the heads of the pH value detection probes (62) and the buffer replenishing gun heads (63) both protrude from the bottom surface of the lower supporting plate (61), and the upper surface of the lower supporting plate (61) is detachably fixedly connected to the output end of the electric push rod (5); The culture dish (7) comprises a bottom shell (71) and an upper cover (72), and the bottom shell (71) and the upper cover (72) are connected by plugging and unplugging; An integrated positioning platform (73) is provided on one side of the bottom shell (71), a monitoring hole (74) is provided on the positioning platform (73) from top to bottom, a liquid inlet hole (76) is provided on one side of the bottom of the positioning platform (73), and the liquid inlet hole (76) is connected to the bottom shell (71) and the monitoring hole (74); An inlet (78) corresponding to the monitoring hole (74) is provided on one side of the surface of the upper cover (72), a liquid supply hole (79) is provided on the surface of the upper cover (72), an atomizing nozzle (05) is fixedly installed on the bottom surface of the upper cover (72), the liquid inlet of the atomizing nozzle (05) is connected to the liquid supply hole (79), the pH value detection probe (62) is inserted into the corresponding monitoring hole (74) through the inlet (78), and the buffer solution replenishing gun head (63) is inserted into the corresponding liquid supply hole (79); a plugging device for blocking the inlet (78) and the liquid supply hole (79) is rotatably installed on the outside of the upper surface of the upper cover (72); The liquid inlet end of the liquid supply pump inside the pump box 1 (13) is fixedly connected to the buffer tank (17), and the liquid outlet end of the liquid supply pump inside the pump box 1 (13) is fixedly connected to each buffer replenishing gun head (63) through an intelligent flow valve; A control box (21) is fixedly mounted on the accessory box (2); The cell culture box (1), the electric push rod (5), the pH value detection probe (62), the buffer replenishing gun head (63), the liquid supply pump and the intelligent flow valve are connected to the intelligent control system inside the control box (21).

2. The intelligent cell adhesion promoting device according to claim 1, characterized in that: The accessory box (2) is also fixedly installed with a pump box 2 (14), a pump box 3 (15), a pump box 4 (16), a carbon dioxide tank (18), an oxygen tank (19) and a disinfectant tank (20), and the pump box 2 (14) is located above the carbon dioxide tank (18), the pump box 3 (15) is located above the oxygen tank (19), and the pump box 4 (16) is located above the disinfectant tank (20); the liquid inlet end of the liquid supply pump inside the pump box 2 (14) is fixedly connected to the carbon dioxide tank (18); the liquid inlet end of the liquid supply pump inside the pump box 3 (15) is fixedly connected to the oxygen tank (19); the liquid inlet end of the liquid supply pump inside the pump box 4 (16) is fixedly connected to the disinfectant tank (20); the liquid outlet ends of the liquid supply pumps inside the pump box 2 (14) and the pump box 3 (15) are both connected to the inside of the cell culture box (1); and the liquid outlet end of the liquid supply pump inside the pump box 4 (16) is connected to the placement table (4).

3. The intelligent cell adhesion promoting device according to claim 2, characterized in that: A carbon dioxide detection sensor (8), an oxygen sensor (9), a carbon dioxide supplement nozzle (10) and an oxygen supplement nozzle (11) are evenly and fixedly installed on one side of the cell culture box (1), and an external exhaust channel (12) is provided on one side of the cell culture box (1), in which an external exhaust fan and an electromagnetic valve are fixedly installed; the carbon dioxide supplement nozzle (10) is fixedly connected to the liquid outlet of the internal liquid supply pump of the pump box second (14) through a pipeline and an intelligent flow valve; the oxygen supplement nozzle (11) is fixedly connected to the liquid outlet of the internal liquid supply pump of the pump box third (15) through a pipeline and an intelligent flow valve; the carbon dioxide detection sensor (8), the oxygen sensor (9), the external exhaust fan and the electromagnetic valve are connected to the intelligent control system inside the control box (21).

4. The intelligent control cell adhesion promoting device according to claim 1, characterized in that: The upper surface of the placement table (4) is evenly provided with a plurality of placement cavities (41) for placing culture dishes (7), and a protrusion (42) is provided on one side of the placement cavity (41). A disinfection cavity (43) is provided in the placement cavity (41) of the placement table (4); the disinfection cavity (43) is fixedly connected to the liquid outlet end of the liquid supply pump inside the pump box (16) through a pipeline; and the culture dish (7) is clamped with the protrusion (42).

5. The intelligent control cell adhesion promoting device according to claim 1, characterized in that: A hollow wiring tray (64) is fixedly installed above the lower support plate (61), and a wire tube 1 (65) and a wire tube 2 (66) are evenly fixedly installed on one side of the hollow wiring tray (64) close to the accessory box (2); a probe control line (67) of the pH value detection probe (62) passes through the hollow wiring tray (64) and the wire tube 1 (65) to be connected to the intelligent control system inside the control box (21); a gun tip control line (68) of the buffer replenishing gun tip (63) passes through the hollow wiring tray (64) and the wire tube 2 (66) to be connected to the intelligent control system inside the control box (21).

6. The intelligent control cell adhesion promoting device according to claim 4, characterized in that: A positioning groove (77) is provided on one side of the lower exterior of the bottom shell (71), and the positioning groove (77) is clamped with the corresponding protrusion (42).

7. The intelligent control cell adhesion promoting device according to claim 6, characterized in that: Two limiting holes (75) are provided on the surface of the positioning platform (73), and the two limiting holes (75) are evenly distributed on both sides of the monitoring hole (74), and the limiting holes (75) are connected to the upper cover (72) by plugging and unplugging.

8. The intelligent control cell adhesion promoting device according to claim 7, characterized in that: Two positioning columns (70) are fixedly mounted on one side of the top surface inside the upper cover (72), and the two positioning columns (70) are evenly distributed on both sides of the entrance (78), and the positioning columns (70) are pluggable connected to the corresponding limiting holes (75).

9. The intelligent control cell adhesion promoting device according to claim 1, characterized in that: The plugging device comprises an elastic sheet (01) and a rotating shaft (04), and a plugging cap head 1 (02) for plugging the liquid supply hole (79) is fixedly mounted on one end of the elastic sheet (01), and a plugging cap head 2 (03) for plugging the inlet (78) is fixedly mounted on the other end of the elastic sheet (01); the elastic sheet (01) is fixedly connected to the top end of the rotating shaft (04), and the bottom end of the rotating shaft (04) is rotatably connected to the upper surface of the upper cover (72), and the rotating shaft (04) is located between the inlet (78) and the liquid supply hole (79); the plugging cap head 1 (02) is connected to the liquid supply hole (79) by plugging and unplugging; the plugging cap head 2 (03) is connected to the inlet (78) by plugging and unplugging.

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