Monascus purpureus PL-HQ8 with high yield of compound enzyme and exopolysaccharide and application of monascus purpureus PL-HQ8

Abstract

The invention relates to the technical field of microorganisms, and particularly discloses monascus purpureus PL-HQ8 with high yield of compound enzyme and exopolysaccharide and application of monascus purpureus PL-HQ8. The monascus purpureus PL-HQ8 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.41598, and the preservation time is November 15, 2024; the invention also provides a fermentation broth, a fermentation supernatant, a compound enzyme preparation, an exopolysaccharide extract and a bacterial suspension prepared from the monascus purpureus PL-HQ8. The monascus purpureus PL-HQ8 provided by the invention can secrete various enzymes and exopolysaccharides, and when the monascus purpureus PL-HQ8 is applied to animal feed, the digestion and absorption capability of animal intestinal tracts on nutrient substances can be enhanced, the animal immunity, the anti-inflammatory and anti-virus capability and the feed utilization rate can be improved, so that the breeding benefits of animals are increased.

Description

Technical Field

[0001] The present application relates to the field of microbial technology, and particularly to a Monascus purpureus PL-HQ8 with high yields of composite enzymes and exopolysaccharides and its applications. Background Art

[0002] As a traditional Chinese medicine, Monascus has a long application history; in recent years, with the development of biotechnology, the research on Monascus has been gradually deepened. In particular, various physiologically active metabolites produced during the fermentation process of Monascus purpureus have attracted wide attention. These metabolites not only have important applications in the pharmaceutical field, but are also widely used in industries such as food fermentation, wine brewing, pigment production, and traditional Chinese medicine manufacturing. The diversity and functionality of Monascus make it an important and indispensable microbial resource in modern industry.

[0003] In order to improve the quality and yield of Monascus fermentation products, researchers have adopted various methods to optimize the fermentation process. Commonly used means include screening excellent strains, adjusting fermentation conditions, adding auxiliary materials, etc.; in addition, some studies have modified Monascus through gene editing technology to enhance its enzyme production ability and the types and contents of metabolites. Although the above methods have improved the yield of Monascus fermentation products to a certain extent, the existing Monascus strains may show unstable states during the fermentation process due to the influence of environmental factors, resulting in large fluctuations in product quality; especially in terms of enzyme production performance and exopolysaccharide production ability, the performance of existing strains is often unsatisfactory, which limits the wide application and development potential of Monascus in industrial production.

[0004] Therefore, it is of great significance to screen out a Monascus purpureus strain with excellent enzyme production performance and exopolysaccharide production ability. Summary of the Invention

[0005] In order to overcome the problem of poor enzyme production performance and exopolysaccharide production ability of existing Monascus purpureus, the present application provides a Monascus purpureus PL-HQ8 with high yields of composite enzymes and exopolysaccharides and its applications.

[0006] In the first aspect, the Monascus purpureus PL-HQ8 provided by the present application adopts the following technical solution: A Monascus purpureus, wherein the Monascus purpureus is Monascus purpureus ( Monascus purpureus) PL-HQ8, which is deposited in the China General Microbiological Culture Collection Center (CGMCC), with the deposit number of CGMCC No. 41598, and the deposit date is November 15, 2024.

[0007] Monascus purpureus PL-HQ8 provided in this application was screened from red yeast rice in Gutian, Fujian. It can secrete various enzymes such as acidic protease, neutral protease, α-amylase, glucoamylase, pectinase, xylanase, β-mannanase, cellulase, etc. and extracellular polysaccharides. Using Monascus purpureus PL-HQ8 provided in this application as a probiotic strain in feed production can, on the one hand, improve the immunity, anti-inflammatory and antiviral abilities of animals and protect animal health; on the other hand, it can also enhance the digestive and absorption ability of animal intestines to nutrients, improve the utilization rate of feed by animals, and greatly increase the breeding efficiency.

[0008] The cultural characteristics of Monascus purpureus PL-HQ8 provided in this application are as Figure 2 shown. The colony is round and membranous with obvious wrinkles and a raised middle. The edge of the mycelium is white. The colony is white at the initial stage, with aerial hyphae on the surface, pink in the middle stage, and gradually turns purple-red in the later stage, and dark red on the back. The morphological characteristics of Monascus purpureus PL-HQ8 are as Figure 3 shown. The diameter of Monascus purpureus hyphae is 2-7 μm, branched. The ascocarp is ellipsoidal, with a diameter of 20-50 μm. Numerous asci are scattered in the ascocarp. The ascospores are oval or spherical. The conidia are produced from the hyphae and are round.

[0009] In the second aspect, this application provides a fermentation broth, and the fermentation broth includes Monascus purpureus PL-HQ8.

[0010] Optionally, the preparation method of the above fermentation broth includes the following steps: (1) Inoculate a single colony of Monascus purpureus PL-HQ8 on a malt agar slant medium, and culture it at 28-35 °C for 4-7 d until the spores are mature; then wash the mature spores with an appropriate amount of physiological saline, put them into an Erlenmeyer flask containing glass beads, shake on a shaker for 2-4 h to disperse the spores; filter the spore suspension with four layers of gauze, dilute it with an appropriate amount of physiological saline, count the spore concentration with a hemocytometer, and adjust the final spore concentration to 10 7 -10 8 cells / mL to obtain a spore suspension; (2) Inoculate the spore suspension into the seed medium at a volume ratio of 5-10%, and culture it at 28-32 °C and 150-200 r / min for 72-96 h to obtain a seed culture solution.

[0011] (3) Inoculate the seed culture solution into the fermentation medium at a volume ratio of 5%-10%. Load 8-10 L of the fermentation medium into a 20 L fermentation tank, sterilize it at 121 °C for 20 min, cool the temperature to 28-32 °C, and the fermentation tank pressure is 0.05 MPa; the initial fermentation control conditions are: temperature 28-30 °C, initial pH of the fermentation medium 5.5-6.0, and air flow rate 0.4-0.6 m 3 / h, stirring speed 250 - 300 r / min, maintaining dissolved oxygen at 20 - 30%; during fermentation, adjust pH by automatically adding ammonia water dropwise to control pH at 5.5 - 6.0; when fermenting for 5 d, adjust the stirring speed to 200 - 250 r / min, and end fermentation when fermenting for 6 d to obtain Monascus purpureus PL - HQ8 fermentation broth.

[0012] In a third aspect, the present application provides a fermentation supernatant, which is the upper clarified liquid obtained after centrifuging the fermentation broth.

[0013] In a fourth aspect, the present application provides a complex enzyme preparation, which includes Monascus purpureus PL - HQ8, the fermentation broth or the fermentation supernatant.

[0014] Optionally, the preparation method of the complex enzyme preparation includes the following steps: centrifuge the Monascus purpureus PL - HQ8 fermentation broth at 8000 - 10000 r / min for 20 - 30 min, and take the supernatant; add ammonium sulfate powder of 70% to the supernatant under ice - bath conditions, precipitate at 4 ± 2 °C for 10 - 12 h, and take the precipitate; redissolve the precipitate with Tris - HCl buffer solution and dialyze at 4 ± 2 °C to obtain the Monascus purpureus PL - HQ8 complex enzyme preparation.

[0015] Optionally, the complex enzyme preparation contains one or more enzymes among acid protease, neutral protease, α - amylase, glucoamylase, pectinase, xylanase, β - mannanase, and cellulase.

[0016] In a fifth aspect, the present application provides an extracellular polysaccharide extract, which is prepared from the fermentation broth or the fermentation supernatant.

[0017] Optionally, the preparation method of the extracellular polysaccharide extract includes the following steps: (1) Centrifuge the Monascus purpureus PL - HQ8 fermentation broth at 8000 - 10000 r / min for 10 - 15 min, discard the precipitate, collect the supernatant to obtain the Monascus purpureus PL - HQ8 fermentation supernatant; (2) Then, slowly add trichloroacetic acid with a concentration of 700 - 900 mg / mL to the fermentation supernatant of Monascus purpureus PL-HQ8 to make its final concentration 40 ± 5 mg / mL. Let it stand at 4 ± 2 °C for 6 - 10 h, then centrifuge at 8000 - 10000 r / min for 10 - 15 min, discard the precipitate, and collect the supernatant. Then, add absolute ethanol to the supernatant according to a volume ratio of 1:(3.5 - 4.5), mix well, place it at 4 ± 2 °C for precipitation for 20 - 24 h, then centrifuge at 8000 - 10000 r / min for 10 - 15 min, and collect the precipitate at the bottom. Finally, dissolve the precipitate in distilled water, centrifuge at 8000 - 10000 r / min for 10 - 15 min, discard the insoluble precipitate at the bottom, and collect the supernatant, which is the extracellular polysaccharide extract of Monascus purpureus PL-HQ8.

[0018] In the sixth aspect, the present application provides a bacterial suspension, and the bacterial suspension includes Monascus purpureus PL-HQ8.

[0019] In the seventh aspect, the present application provides the use of Monascus purpureus PL-HQ8, fermentation broth, fermentation supernatant, composite enzyme preparation, extracellular polysaccharide extract or bacterial suspension in animal feed or animal feed additives.

[0020] In summary, the present application has the following beneficial effects: 1. The present application provides a Monascus purpureus Monascus purpureus) PL-HQ8, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number CGMCC No. 41598, and the preservation time is November 15, 2024.

[0021] 2. The Monascus purpureus PL-HQ8 provided by the present application can secrete various enzymes such as acid protease, neutral protease, α-amylase, glucoamylase, pectinase, xylanase, β-mannanase, and cellulase. Using the Monascus purpureus PL-HQ8 provided by the present application as a probiotic strain in feed, the enzymes produced by Monascus purpureus PL-HQ8 can promote the rupture of the cell walls of the components in animal feed, improve the degradation rate of cell walls, promote the full digestion and absorption of animal nutrients, and thus improve the growth performance of animals and increase the breeding efficiency.

[0022] 3. The Monascus purpureus PL-HQ8 provided by the present application can secrete extracellular polysaccharides, and the yield of extracellular polysaccharides is high. Using the above Monascus purpureus PL-HQ8 as a probiotic strain in feed, the extracellular polysaccharides produced by it have good antioxidant properties, and thus can enhance the immunity, anti-inflammatory and antiviral abilities of animals, protect the health of animals, and thus reduce the breeding cost and improve the breeding efficiency. Description of the Drawings

[0023] Figure 1It is the phylogenetic tree of 18S rDNA of Monascus purpureus PL-HQ8; Figure 2 It is the culture characteristic diagram of Monascus purpureus PL-HQ8; Figure 3 It is the morphological characteristic diagram of Monascus purpureus PL-HQ8. Specific implementation manners

[0024] The following further elaborates on the present application in conjunction with the accompanying drawings and embodiments. It should be specifically noted that: for those conditions not specified in the following embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. Except for special instructions, the raw materials used in the following embodiments can all be obtained from ordinary commercial sources.

[0025] The present application provides a Monascus purpureus PL-HQ8, which is screened from red yeast rice in Gutian, Fujian. Through 18S rDNA identification, this strain is Monascus purpureus ( Monascus purpureus) , named Monascus purpureus PL-HQ8, is preserved in the China General Microbiological Culture Collection Center (CGMCC), with the preservation number CGMCC No. 41598, and the preservation time is: November 15, 2024.

[0026] The culture media and raw material sources involved in the embodiments of the present application are as follows: Composition of the enrichment medium: malt extract powder 130.0 g / L, chloramphenicol 0.1 g / L; Composition of the malt agar medium: malt extract powder 130.0 g / L, chloramphenicol 0.1 g / L, agar 15.0 g / L; Composition of the seed medium: malt extract powder 130.0 g / L, potassium dihydrogen phosphate 1.0 g / L, magnesium sulfate 0.5 g / L, ferric sulfate 0.01 g / L; Composition of the fermentation medium: malt extract powder 50.0 g / L, peptone 20.0 g / L, yeast extract powder 10.0 g / L, potassium dihydrogen phosphate 2.0 g / L, magnesium sulfate 0.5 g / L, ferric sulfate 0.01 g / L, manganese sulfate 0.006 g / L, zinc sulfate 0.006 g / L.

[0027] The following further elaborates on the present application in conjunction with the embodiments, performance detection tests and the description of the accompanying drawings. Example 1 Isolation, screening and identification of the strain

[0028] (1) Isolation and screening: Use Fujian Gutian red yeast rice as the sample to screen Monascus; take 1 g of sample in 100 mL of enrichment medium, fully shake at 180 r / min and 30°C for 24 h, take the dilution to spread on malt agar plate, invert and culture at 30°C for 7-10 days to observe the growth of colonies; pick a single colony and perform plate streak purification for more than 3 times to obtain a purified strain; The culturing characteristics of the purified strains are as follows: Figure 2 As shown in the figure, the colony is circular and membrane-like with obvious wrinkles and a bulge in the middle. The edge of the mycelium is white. The colony is white in the early stage, with aerial hyphae on the surface, light red in the middle stage, and gradually turns purple-red in the late stage. The back is dark red. Figure 3 As shown, the diameter of the hyphae is 2-7um, branched, and septate. The cleistothecia are ellipsoidal, with a diameter of 20-50um. There are many asci scattered in the cleistothecia. The ascospores are ovoid or spherical. The conidia are produced by the hyphae and are round. (2) Identification: The purified strain was inoculated into enrichment medium and cultured at 30°C for 24 h for morphological identification and 18SrDNA molecular biological identification. The strain was sequenced and identified by Beijing Liuhe BGI Technology Co., Ltd. The sequence results were compared with the sequences in Genbank by Blast analysis, and a phylogenetic tree was constructed using Mega 6.0 software, such as Figure 1 .

[0029] Figure 1 The results showed that the purified strain was homologous to the Monascus purpureus published by NCBI. Combined with the traditional morphological characteristics, the isolated bacteria was further identified as Monascus purpureus, and Monascus purpureus was named PL-HQ8. Monascus purpureus PL-HQ8 was deposited in the General Microbiology Center of China Microbiological Culture Collection Administration, with the deposit number: CGMCC No.41598 and the deposit date: November 15, 2024. Example 2

[0030] Embodiment 2 provides a Monascus PL-HQ8 fermentation broth, and its preparation method comprises the following steps: (1) A single colony of Monascus PL-HQ8 was inoculated into a malt agar slant medium and cultured at 30°C for 5 days until the spores matured. The mature spores were then washed with an appropriate amount of physiological saline and placed in a triangular flask containing glass beads. The spores were shaken on a shaker for 2 hours to break up the spores. The spore suspension was filtered through four layers of gauze, diluted with an appropriate amount of physiological saline, and the spore concentration was counted using a hemocytometer. The final spore concentration was adjusted to 10 8 / mL, and a spore suspension of Monascus PL-HQ8 was obtained; (2) Inoculate the spore suspension of Monascus PL-HQ8 into the seed culture medium at a volume ratio of 8%, and culture at 30°C and 180 r / min for 96 h to prepare a seed culture solution; (3) Charge 10 L of fermentation medium into a 20-L fermenter, sterilize it at 121 °C for 20 min, cool the temperature to 28 - 30 °C, inoculate the seed culture solution into the fermenter at a volume ratio of 8%, and keep the pressure in the fermenter at 0.05 MPa; the initial fermentation control conditions are: temperature 28 - 30 °C, initial pH of the fermentation medium 5.5 - 6.0, air flow rate 0.4 - 0.6 m 3 / h, stirring speed 280 r / min, and keep the dissolved oxygen at 25%; during fermentation, adjust the pH by automatically adding ammonia water, and control the pH at 5.5 - 6.0; on the 5th day of fermentation, adjust the stirring speed to 230 r / min, and end the fermentation on the 6th day of fermentation to obtain the Monascus purpureus PL-HQ8 fermentation broth. Example 3

[0031] Example 3 provides a Monascus purpureus PL-HQ8 complex enzyme preparation, and its preparation method includes the following steps: Centrifuge the Monascus purpureus PL-HQ8 fermentation broth obtained in Example 2 at 10,000 r / min for 20 min, and take the supernatant; add 70% ammonium sulfate powder to the supernatant under ice bath conditions, precipitate at 4 °C for 12 h, and take the precipitate; redissolve the precipitate with Tris-HCl buffer solution and dialyze at 4 °C to obtain the Monascus purpureus PL-HQ8 complex enzyme preparation. Comparative Example 1

[0032] Comparative Example 1 provides a Monascus purpureus Mp-42 fermentation broth, and its preparation method includes the following steps: (1) Inoculate the Mp-42 strain into the starch separation medium, culture it at 30 °C for 7 d, punch out 6 mycelial cakes with a 0.9-cm puncher and inoculate them into 50 mL of seed medium, and culture them on a shaker at 180 r / min for 3 d; then wash the mature spores with an appropriate amount of normal saline, put them into a triangular flask containing glass beads, shake on a shaker for 2 h to disperse the spores; filter the spore suspension with four layers of gauze, dilute it with an appropriate amount of normal saline, count the spore concentration with a hemocytometer, and adjust the final spore concentration to 10 8 cells / mL to obtain the Monascus purpureus Mp-42 spore suspension; (2) Inoculate the Monascus purpureus Mp-42 spore suspension into the fermentation medium at a volume ratio of 8%, and culture it on a shaker at 30 °C and 180 r / min for 7 d to obtain the Monascus purpureus Mp-42 fermentation broth. Comparative Example 2

[0033] Comparative Example 2 provides a Monascus purpureus Mp-42 complex enzyme preparation, and its preparation method includes the following steps: The Monascus purpureus PL-HQ8 fermentation broth obtained in Comparative Example 1 was centrifuged at 8000 r / min for 10 min, and the supernatant was taken; 70% ammonium sulfate powder was added to the supernatant under ice bath conditions, and precipitation was carried out at 4 °C for 12 h, and the precipitate was taken; the precipitate was redissolved with Tris-HCl buffer solution and dialyzed at 4 °C to obtain the Monascus purpureus Mp-42 complex enzyme preparation. Detection of enzyme production performance

[0034] The enzyme activities of protease, α-amylase, glucoamylase, cellulase, β-mannanase, pectinase, and xylanase in the complex enzyme preparations obtained in Example 3 and Comparative Example 2 were detected, and the detection results are shown in Table 1 below.

[0035] The determination of protease activity was carried out according to the protease activity determination method of GB / T 28715-2012; the α-amylase activity was detected according to the α-spectrophotometry method in DB 13T1095-2009 for enzyme preparations for feed; the glucoamylase activity was detected according to the general test method for industrial enzyme preparations of QB / T1803-1993; the cellulase activity was detected according to the spectrophotometry method in the determination of cellulase activity in NY / T 912-2004 for feed additives; the β-mannanase activity was detected according to the determination method of β-mannanase activity in GB / T 36861-2018 for feed additives; the pectinase activity was detected according to the method for determining pectinase activity in pectinase preparations of QB 1502-92; the xylanase activity was detected according to the xylanase activity spectrophotometry method in GB / T 23874-2009 for feed additives.

[0036] Table 1 Detection results of the activities of various enzymes in the complex enzyme preparations obtained in Example 3 and Comparative Example 2

[0037] According to the detection results in Table 1, it can be seen that the Monascus purpureus PL-HQ8 provided in this application can simultaneously produce acid protease, neutral protease, α-amylase, glucoamylase, pectinase, xylanase, β-mannanase, and cellulase through one fermentation; the glucoamylase enzyme activity of the obtained complex enzyme preparation is as high as 1513 U / mL, the pectinase enzyme activity is as high as 929 U / mL, and the acid protease enzyme activity is as high as 356.8 U / mL. Therefore, it shows that the Monascus purpureus PL-HQ8 provided in this application can be used as a probiotic strain in feed, promote the degradation of feed, improve the absorption of feed nutrients by animals, improve the growth performance of animals, and thus increase the breeding efficiency. Example 4

[0038] Example 4 provides an extracellular polysaccharide extract of Monascus purpureus PL-HQ8, and its preparation method includes the following steps: (1) Centrifuge the Monascus purpureus PL-HQ8 fermentation broth obtained in Example 2 at 10,000 r / min for 10 min, discard the precipitate, collect the supernatant, and obtain the Monascus purpureus PL-HQ8 fermentation supernatant; (2) Then slowly add trichloroacetic acid with a concentration of 800 mg / mL to the Monascus purpureus PL-HQ8 fermentation supernatant to make its final concentration 40 mg / mL, let it stand at 4 °C for 8 h, then centrifuge at 10,000 r / min for 10 min, discard the precipitate, and collect the supernatant; then add absolute ethanol to the supernatant according to a volume ratio of 1:4, mix well and place it at 4 °C for precipitation for 24 h, then centrifuge at 10,000 r / min for 10 min, and collect the precipitate at the bottom; finally dissolve the precipitate in distilled water, centrifuge at 10,000 r / min for 10 min, discard the insoluble precipitate at the bottom, and collect the supernatant, which is the extracellular polysaccharide extract of Monascus purpureus PL-HQ8. Comparative Example 3

[0039] Comparative Example 3 provides an extracellular polysaccharide extract of Monascus purpureus Mp-42.

[0040] The above extracellular polysaccharide extract of Monascus purpureus Mp-42 is prepared from the Monascus purpureus Mp-42 fermentation broth provided in Comparative Example 2, and the preparation method is the same as that of Example 4. Performance detection

[0041] Detect the extracellular polysaccharide production performance and antioxidant performance of the extracellular polysaccharide extracts obtained in Example 4 and Comparative Example 3, and the results are shown in Table 2 below.

[0042] (1) Detect the extracellular polysaccharide content in the extracellular polysaccharide extract by the phenol-sulfuric acid method; (2) Antioxidant performance: Centrifuge the extracellular polysaccharide solution at 10,000 r / min for 10 min to retain the precipitate, dry it at 80 °C, weigh it, and then prepare a polysaccharide solution with a concentration of 0.5 mg / mL with purified water for the following detection tests; Determination of the activity of scavenging DPPH free radicals: Take 2 mL of the polysaccharide solution and add 2 mL of DPPH solution (concentration is 1×10 - 4 mol / L), mix well, measure its absorbance Ai at 517 nm after reacting at room temperature for 30 min; at the same time, measure the absorbance A0 of the mixture of 2 mL of DPPH solution + 2 mL of ethanol and the absorbance Aj of the mixture of 2 mL of polysaccharide solution + 2 mL of ethanol. Each experiment is done in parallel 3 times and take the average value. The calculation formula for the DPPH free radical scavenging rate is: DPPH free radical scavenging rate (%) = (1 - (Ai - Aj) / A0) × 100% Determination of the activity of scavenging hydroxyl radicals: Take 1 mL of 8.8 mmol / L salicylic acid, and successively add 1 mL of PBS buffer solution and 1 mL of polysaccharide solution. Mix well, then add 1 mL of 9 mmol / L FeSO 4 and 1 mL of 9 mmol / L H 2 O 2 . React at 37 °C for 60 min, take the supernatant, and then detect the absorbance value A i at 510 nm; measure the absorbance value A 0 with 1 mL of distilled water replacing the polysaccharide solution; replace H 2 O 2 with distilled water to measure the absorbance value A j . Each experiment is performed in parallel 3 times, and the average value is taken. The calculation formula for the hydroxyl radical scavenging rate is: Hydroxyl radical scavenging rate (%) = ((1 - (Ai - Aj) / A0) × 100% Scavenging superoxide anion radicals: Take 4.5 mL of 0.05 mol / L Tris-HCl buffer solution (pH = 8.2), preheat it in a water bath at 25 °C for 20 min, and add 4.5 mL of polysaccharide solution and 0.5 mL of 25 mmol / L pyrogallol solution respectively; mix well, react in a water bath at 25 °C for 5 min, then add 1 mL of 8 mol / L HCl to terminate the reaction, let it stand for 10 min, and measure the absorbance value A i at 299 nm; measure the absorbance value A 0 with 1 mL of distilled water replacing the polysaccharide solution; measure the absorbance value A j with 0.5 mL of 10 mmol / L HCl replacing the pyrogallol solution. Each experiment is performed in parallel 3 times, and the average value is taken. The calculation formula for the superoxide anion radical scavenging rate is: Superoxide anion radical scavenging rate (%) = (1 - (Ai - Aj) / A0) × 100% Table 2 Detection results of the extracellular polysaccharide content in the extracellular polysaccharide extracts obtained in Example 4 and Comparative Example 3

[0043] According to the detection results in Table 2, it can be seen that the ability of Monascus purpureus PL-HQ8 provided by this application to produce extracellular polysaccharides is significantly better than that of the existing Monascus purpureus Mp-42. The scavenging ability (antioxidant ability) of Monascus purpureus PL-HQ8 against DPPH radicals, hydroxyl radicals and superoxide radicals is also better than that of Monascus purpureus Mp-42. Therefore, Monascus purpureus PL-HQ8 provided by this application can produce extracellular polysaccharides, has good antioxidant effects, and when used in feed, can improve the immunity of animals, protect the health of animals, and improve the breeding efficiency.

[0044] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made thereto based on the present invention, which will be obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of the present invention claimed.

Claims

1. A Monascus purpurogenum, characterized in that: The Monascus purpurogenum is Monascus purpurogenum ( Monascus purpureus PL-HQ8 is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC), with the deposit number CGMCC No. 41598 and the deposit date is November 15, 2024.

2. A fermentation broth, characterized in that: The fermentation broth comprises the Monascus purpurogenum PL-HQ8 according to claim 1.

3. A fermentation supernatant, characterized in that: The fermentation supernatant is the upper clear liquid obtained after centrifuging the fermentation broth according to claim 2.

4. A composite enzyme preparation, characterized in that: The composite enzyme preparation comprises the Monascus purpurogenum PL-HQ8 according to claim 1, the fermentation broth according to claim 2 or the fermentation supernatant according to claim 3.

5. The complex enzyme preparation according to claim 4 is characterized in that: The complex enzyme preparation comprises one or more enzymes selected from the group consisting of acidic protease, neutral protease, α-amylase, saccharifying enzyme, pectinase, xylanase, β-mannanase and cellulase.

6. An extracellular polysaccharide extract, characterized in that The extracellular polysaccharide extract is prepared from the fermentation broth according to claim 2 or the fermentation supernatant according to claim 3.

7. A bacterial suspension, characterized in that: The bacterial suspension comprises the Monascus purpurogenum PL-HQ8 described in claim 1.

8. Use of the Monascus purpurogenum PL-HQ8 according to claim 1, the fermentation broth according to claim 2, the fermentation supernatant according to claim 3, the complex enzyme preparation according to claim 4 or 5, the extracellular polysaccharide extract according to claim 6 or the bacterial suspension according to claim 7 in animal feed or animal feed additives.

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