Camel milk-derived exosome freeze-drying and preparation method, medium and system

Abstract

The invention provides a camel milk-derived exosome freeze-drying and preparation method, a medium and a system.The preparation method comprises the steps that a chymosin solution is prepared and subjected to chymosin emulsification, cell debris is removed to collect a camel milk middle layer, pH is adjusted to obtain a middle layer solution, the chymosin solution is added into the middle layer solution for chymosin incubation, and the camel milk-derived exosome freeze-drying solution is obtained; and centrifuging, filtering and freeze-drying the incubated mixed solution to obtain the camel milk exosome freeze-dried powder. The method has the advantages of being high in extraction efficiency, high in purity, easy and convenient to operate and the like, and research and application of the camel milk source exosome can be promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical development of camel milk, and specifically, to a freeze-drying method, preparation method, medium and system for camel milk-derived exosomes. Background Art

[0002] Exosomes can be secreted by various cells. As an important carrier for intercellular communication, they can transfer various specific biomolecules such as nucleic acids, proteins, enzymes, and lipids to recipient cells and play an important role in the regulation of biological processes. The prerequisite for studying the components inside exosomes is to extract them with high quality. Due to the small particle size of exosomes and their mixing with various other biomolecules, it is difficult to separate and purify them, which hinders further research on them.

[0003] Camel milk is known as the "desert platinum". In addition to being rich in nutrients, camel milk also has the advantages of small fat globules, easy digestion, and blood sugar reduction. In addition, it also contains a variety of bioactive factors and lysozyme, and these nutrients have the effects of sterilizing and inhibiting bacteria and enhancing immune function. In order to give full play to the medical value of camel milk, exosomes derived from camel milk are extracted to exert their nano-level biological regulation effect.

[0004] Currently, the preparation methods for milk-derived exosomes are mostly based on differential centrifugation, with complex extraction steps and relatively low extraction efficiency.

[0005] This situation has to a certain extent restricted the in-depth development of research on camel milk exosomes and their popularization in practical applications. Summary of the Invention

[0006] The present invention provides a freeze-drying preparation method for camel milk-derived exosomes to solve the technical problem of low efficiency in centrifugally extracting exosomes from camel milk in the prior art.

[0007] To this end, the specific technical solution adopted by the present invention is as follows:

[0008] A freeze-drying preparation method for camel milk-derived exosomes, comprising the following steps,

[0009] Prepare a rennet solution and perform rennet emulsification, remove cell debris to collect the middle layer of camel milk and adjust the pH to obtain a middle layer solution, add the rennet solution to the middle layer solution for rennet incubation, and centrifuge, filter, and freeze-dry the incubated mixture to obtain freeze-dried powder of camel milk exosomes.

[0010] In a possible design, the steps of preparing the chymosin solution and performing chymosin emulsification are specifically as follows: Prepare according to the following ratio. Dissolve 0.035 mg of chymosin in 1 L of sodium chloride solution with a mass concentration of 1% to prepare a chymosin solution with a concentration of 0.035 mg / L. Place the chymosin solution in a water bath at 37 ± 0.5 °C for activation for 30 min.

[0011] In a possible design, the steps of removing cell debris to collect the middle layer of camel milk and adjusting the pH to obtain the middle layer solution are specifically as follows: Centrifuge the camel milk at 4 °C and 2000 r / min for 15 min using a refrigerated centrifuge, collect the middle layer, add glacial acetic acid solution to the collected middle layer, and adjust the pH value to 6.0 ± 0.05 to obtain the middle layer solution.

[0012] In a possible design, the steps of adding the chymosin solution to the middle layer solution for chymosin incubation are specifically as follows: Take the activated chymosin solution and add it to the middle layer solution after adjusting the pH value, and mix thoroughly; Incubate the mixture in a water bath at 37 ± 0.5 °C for 30 - 35 min.

[0013] In a possible design, in the steps of adding the chymosin solution to the middle layer solution for chymosin incubation, the following ratio is used: 2.5 ml of chymosin solution needs to be added per 50 ml of camel milk.

[0014] In a possible design, in the steps of removing cell debris to collect the middle layer of camel milk and adjusting the pH to obtain the middle layer solution, the mass concentration of the glacial acetic acid solution is 10%.

[0015] The present invention also provides a camel milk-derived exosome, which is extracted by using the aforementioned freeze-drying preparation method.

[0016] The present invention also provides a computer-readable storage medium, which stores computer-executable instructions for causing a computer to execute the aforementioned freeze-drying preparation method of camel milk-derived exosomes.

[0017] The present invention also provides a freeze-drying preparation system for camel milk-derived exosomes, including a memory, a control processor, and a computer program stored on the memory and executable on the control processor. The control processor executes the program to implement the aforementioned freeze-drying preparation method of camel milk-derived exosomes.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] 1. Yield improvement: By reducing the extraction of impurities and residual cells, this method can improve the yield of exosomes and obtain more target products.

[0020] 2. Quality improvement: Since the exosome isolation and purification technology from camel milk can effectively remove impurities and cell residues in exosomes, thus obtaining a purer exosome product, its quality is greatly improved.

[0021] 3. Precision improvement: By optimizing the separation and purification process, this method can obtain a more accurate exosome product, further improving the precision of exosome isolation and purification.

[0022] 4. Efficiency improvement: Compared with traditional exosome extraction methods, the freeze-drying preparation technology of camel milk-derived exosomes has the advantages of simple operation, without the need to use complex equipment and reagents such as a group of centrifuge tubes, thus improving the extraction efficiency.

[0023] 5. Facilitating storage and transportation: Compared with traditional methods, the volume of exosomes is greatly reduced after freeze-drying by this method, making it convenient to carry and transport. At the same time, due to its stable solid form, the loss and risk during transportation are reduced, and the transportation cost is lowered.

[0024] The preparation method of the present invention is simple to operate and easy to realize industrial production.

[0025] Through specific centrifugation, filtration and freeze-drying steps, the yield of camel milk-derived exosomes can be increased. Through experimental comparison, compared with the traditional freeze-drying method, after storage at -20°C for 168 days, the exosomes extracted by this method can still maintain a large number of vesicle structures and quantities.

[0026] Based on the above points, this technology has the advantages of high extraction efficiency, high purity, simple operation, etc., and will help to promote the research and application of exosomes from camel milk. Brief Description of the Drawings

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present application. For those skilled in the art, without creative efforts, other drawings can be obtained according to these drawings.

[0028] Figure 1 It is a schematic diagram of TEM detection for an embodiment of the present invention;

[0029] Figure 2 It is a schematic diagram of TEM detection for a control example;

[0030] Figure 3 It is a schematic diagram of NTA detection for an embodiment of the present invention;

[0031] Figure 4 It is a schematic diagram of NTA detection for a control example;

[0032] Figure 5 Schematic diagram of the NTA detection results of the embodiments of the present invention;

[0033] Figure 6 Schematic diagram of the NTA detection results of the control example;

[0034] Figure 7 Schematic diagram of the WB detection results of the embodiments and control example of the present invention;

[0035] Figure 8 Schematic diagram of the NTA detection of the control example after storage at -20°C for three months and dissolved in equal proportion;

[0036] Figure 9 Schematic diagram of the NTA detection of the embodiments of the present invention after storage at -20°C for three months and dissolved in equal proportion;

[0037] Figure 10 Process flow chart of the embodiments of the present invention. Detailed implementation manners

[0038] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Usually, the components of the embodiments of the present invention described and illustrated herein can be arranged and designed in various different configurations.

[0039] Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0040] To further illustrate each embodiment, the present invention provides accompanying drawings, which are part of the disclosure of the present invention. They are mainly used to illustrate the embodiments and can be combined with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, those of ordinary skill in the art should be able to understand other possible implementation manners and the advantages of the present invention. The components in the drawings are not drawn to scale, and similar component symbols are usually used to represent similar components.

[0041] It should be noted that, without conflict, the features in the embodiments of the present invention can be combined with each other.

[0042] As Figure 10 shown, the freeze-drying preparation method of camel milk-derived exosomes of the present invention includes the following steps:

[0043] Step S1, Prepare chymosin solution: Dissolve 0.035 mg of chymosin in 1 L of sodium chloride solution with a mass concentration of 1% to prepare a chymosin solution with a concentration of 0.035 mg / L.

[0044] Step S2, Activate chymosin solution: Place the chymosin solution in a water bath at 37 ± 0.5 °C for 30 min for activation. After multiple experiments, it is verified that the activated chymosin solution is relatively stable and will not form liquid stratification under this condition.

[0045] Step S3, Centrifuge camel milk and collect the middle layer: Centrifuge 50 mL of camel milk at 4 °C and 2000 r / min for 15 min using a refrigerated centrifuge, and carefully collect the middle layer with a sterile pipette, avoiding disturbing other layers. Through this step, a specific material layer can be effectively separated. After repeated experiments, the average volume of the collected middle layer is 28 ± 3 mL.

[0046] Step S4, Adjust pH value: Add 10% glacial acetic acid solution to the collected middle layer and adjust the pH value to 6.0 ± 0.05. At this pH value, it is beneficial for the subsequent steps. After repeated experiments, the pH value of the adjusted solution is highly stable, with a deviation not exceeding ±0.03.

[0047] Step S5, Mix the solutions and incubate: Take 2.5 mL of the activated chymosin solution and add it to the solution obtained in Step S4, and mix thoroughly. Incubate the mixture in a water bath at 37 ± 0.5 °C for 30 - 35 min.

[0048] Step S6, Centrifuge the mixed solution again: Centrifuge the incubated mixed solution at 2000 r / min for 20 min at 4 °C. This step can remove the precipitate.

[0049] Step S7, Centrifuge the supernatant: Centrifuge the supernatant from Step S6 at 20000 r / min for 30 min at 4 °C. This step can remove macromolecules.

[0050] Step S8, Filter the supernatant: Filter the supernatant obtained in Step S7 using a 2 - µm syringe filter. This step can remove large vesicles.

[0051] Step S9, Freeze - dry to remove water: Freeze - dry the supernatant obtained in Step S8 at 50 °C and 0.35 mBar. The quality of the freeze - dried product is stable, and the water content is reduced to 0.001. This step can remove the liquid.

[0052] Step S10, Collect the freeze - dried powder: Centrifuge the precipitate obtained in Step S9 at 20000 r / min for 15 min at 4 °C, and finally collect the freeze - dried powder of camel milk exosomes. This step can remove salt deposits.

[0053] The present invention also provides an exosome derived from camel milk, which is obtained by using the aforementioned freeze-drying preparation method.

[0054] The present invention also provides a computer-readable storage medium storing computer-executable instructions for causing a computer to execute the aforementioned freeze-drying preparation method of camel milk-derived exosomes.

[0055] The present invention also provides a freeze-drying preparation system for camel milk-derived exosomes, including a memory, a control processor, and a computer program stored on the memory and executable on the control processor. The control processor executes the program to implement the aforementioned freeze-drying preparation method of camel milk-derived exosomes.

[0056] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0057] 1. Yield improvement: By reducing the extraction of impurities and residual cells, this method can improve the yield of exosomes and obtain more target products.

[0058] 2. Quality improvement: Since the separation and purification technology of exosomes derived from camel milk can effectively remove impurities and cell residues in exosomes, thus obtaining a more pure exosome product, its quality is greatly improved.

[0059] 3. Precision improvement: By optimizing the separation and purification process, this method can obtain a more accurate exosome product, further improving the precision of exosome separation and purification.

[0060] 4. Efficiency improvement: Compared with the traditional exosome extraction method, the freeze-drying preparation technology of camel milk-derived exosomes has the advantages of simple operation, without the need to use complex equipment and reagents such as a group of centrifuge tubes, thus improving the extraction efficiency.

[0061] 5. Facilitating storage and transportation: Compared with the traditional method, the volume of exosomes is greatly reduced after freeze-drying by this method, which is convenient for carrying and transportation. At the same time, due to its stable solid form, the loss and risk during transportation are reduced, and the transportation cost is lowered.

[0062] Based on the above points, this technology has the advantages of high extraction efficiency, high purity, and simple operation, which will contribute to promoting the research and application of exosomes derived from camel milk.

[0063] Control example:

[0064] Conventional freeze-drying method: Take 1.8 mL of milk and centrifuge it at 2000×g for 10 minutes at room temperature to remove milk fat globules. Then, take about 1 mL of the middle layer liquid and centrifuge it again at 10000×g for 30 minutes to remove impurities, obtaining 0.8 mL of supernatant, which is whey. Add an equal volume of PBS to 400 μL of whey and mix well, then add 400 μL of reagent and incubate for 30 minutes. Centrifuge at 10000×g for 10 minutes, and the exosomes precipitate at the bottom of the tube. Place the exosome sample in a vacuum freeze-dryer for freeze-drying treatment to sublime the water in the sample under a vacuum environment to achieve the purpose of drying.

[0065] Experimental analysis:

[0066] 1. TEM detection of exosomes

[0067] Drop the resuspensions of exosomes extracted by two different methods onto three 300-mesh copper grids respectively, and place them in a fume hood to stand and dry. Drop phosphotungstic acid dye on the sealing film, invert the copper grid with the sample on the sealing film, and stain for 1.5 min. Immediately wash the copper grid with ddH 2 2O, dry it, and place it under TEM. Observe the morphology, diameter size, and membrane structure of exosomes under the conditions of 80 - 120 kV.

[0068] As Figure 1-2 shown, the TEM detection results: To determine the quantity and structure of camel milk-derived exosomes, count the exosomes with cup-shaped structures using TEM. The results are as Figure 1-2 shown. As Figure 1-2 can be seen, the shape of the exosomes extracted by the chymosin-assisted method is more in line with the cup-shaped morphology, and the double-layer membrane structure is clearer. The structures of the exosomes obtained by the conventional freeze-drying method are damaged to varying degrees.

[0069] 2. NTA detection

[0070] As Figure 3-6 shown, to determine the particle size and concentration of camel milk-derived exosomes, use NTA for particle size detection. Turn on the nano particle size and Zeta potential analyzer, and start the Zetasizer software after the laser is stable. Pipette 10 μL of the exosome solution extracted by two different methods, dilute it to 1 mL with PBS filtered through a 0.1 μm needle filter, mix well with a vortex mixer, and then slowly inject the sample into the sample cell with a syringe. Measure the particle size of the exosomes and record the detection results (see Table 1). The temperature needs to be maintained at 23 - 37 °C during the measurement process.

[0071] Table 1 Peak Analysis (Concentration)

[0072] Diameter / nm Particles / mL FWHM / nm Percentage Ordinary freeze-drying method 147.2 3.7E+6 97.7 91.0 Chymosin freeze-drying method 108.3 3.1E+6 59.2 100.0

[0073] The extraction efficiency is compared in the following table:

[0074] Average yield (mg / L) Ordinary freeze-drying method 312.68±26.35 Chymosin freeze-drying method 498.42±31.26

[0075] like Figure 8-9 As shown, after being stored at -20°C for 3 months, the exosomes prepared in the embodiment of the present invention had more vesicle particles than those in the control example.

[0076] 3. WB detection

[0077] like Figure 7 As shown, according to Exo-IsolationTM Exosome Protein Extraction Kit Instructions for Use Extract exosome protein and use the Bio-Tech BCA Protein Quantification Kit to determine the exosome protein concentration. The mass of the exosome solution extracted by the two different methods was unified as 50ug for loading, separated by 10% SDS-PAGE (voltage 80V, changed to 120V when the band reached the separation gel), and then transferred to a PVDF membrane and blocked with TBST solution containing 5% skim milk powder at room temperature for 1h; primary antibodies CD63 (abcam, ab68418) (1:1000 dilution), CD81 (abcam, ab109201) (1:1000 dilution), TSG101 (abcam, ab133586) (1:1000 dilution) were added, and incubated at 4°C overnight; TBST was washed 3 times, 5min each time, and the corresponding secondary antibody (1:3000 dilution) was added, and incubated at room temperature for 1h; TBST was washed 3 times, 5min each time, and treated with ECL chemiluminescent solution, and developed and photographed with the Tianneng chemiluminescent imaging system.

[0078] Examples of computer-readable storage media include: read-only memory (ROM), programmable read-only memory (PROM), electrically erasable programmable read-only memory (EEPROM), random access memory (RAM), dynamic random access memory (DRAM), static random access memory (SRAM), flash memory, non-volatile memory, CD-ROM, CD-R, CD+R, CD-RW, CD+RW, DVD-ROM, DVD-R, DVD+R, DVD-RW, DVD+RW, DVD-RAM, BD-ROM, BD-R, BD-R LTH, BD-RE, Blu-ray or optical disc storage, hard disk drive (HDD), solid state drive (SSD), card memory (such as, multimedia card, secure digital (SD) card, or extreme digital (XD) card), magnetic tape, floppy disk, magneto-optical data storage media, optical data storage media, hard disk, solid state disk, and any other medium that is configured to store a computer program and any associated data, data files, and data structures in a non-transitory manner and to provide the computer program and any associated data, data files, and data structures to a processor or computer such that the processor or computer can execute the computer program. In one example, the computer program and any associated data, data files, and data structures are distributed across a networked computer system such that the computer program and any associated data, data files, and data structures are stored, accessed, and executed in a distributed fashion by one or more processors or computers.

[0079] Although the foregoing methods are illustrated and described as a series of acts for simplicity of explanation, it should be understood and appreciated that the methods are not limited by the order of the acts, since according to one or more embodiments, some acts may occur in different orders and / or concurrently with other acts not illustrated and described herein or other acts that would be understood by those skilled in the art. Those skilled in the art will further appreciate that the various illustrative logical blocks, modules, circuits, and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware, computer software, or combinations of both. To clearly illustrate this interchangeability of hardware and software, the various illustrative components, blocks, modules, circuits, and steps are described above in terms of their functionality. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the overall system. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present invention. The various illustrative logical blocks, modules, and circuits described in connection with the embodiments disclosed herein may be implemented using a general purpose processor, a digital signal processor (DSP), an application specific integrated circuit (ASIC), a field programmable gate array (FPGA) or other programmable logic device, discrete gate or transistor logic, discrete hardware components, or any combination thereof designed to perform the functions described herein. A general purpose processor may be a microprocessor, but in the alternative, the processor may be any conventional processor, battery compartment control board, micro battery compartment control board, or state machine. The processor may also be implemented as a combination of computing devices, such as a combination of a DSP and a microprocessor, multiple microprocessors, one or more microprocessors cooperating with a DSP core, or any other such configuration. The steps of a method or algorithm described in connection with the embodiments disclosed herein may be embodied directly in hardware, in a software module executed by a processor, or in a combination of both. The software module may reside in RAM memory, flash memory, ROM memory, EPROM memory, EEPROM memory, registers, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to the processor such that the processor can read information from, and write information to, the storage medium. In the alternative, the storage medium may be integral to the processor. The processor and the storage medium may reside in an ASIC. The ASIC may reside in a user terminal. In the alternative, the processor and the storage medium may reside as discrete components in a user terminal. In one or more exemplary embodiments, the described functionality may be implemented in hardware, software, firmware, or any combination thereof. If implemented in software as a computer program product, the functions may be stored on or transmitted via a computer readable medium as one or more instructions or code.A computer-readable medium includes both computer storage media and communication media, including any medium that facilitates the transfer of a computer program from one place to another. The storage media can be any available media accessible by a computer. By way of example and not limitation, such computer-readable media can include RAM, ROM, EEPROM, CD-ROM or other optical disk storage, magnetic disk storage or other magnetic storage devices, or any other medium that can be used to carry or store desired program code in the form of instructions or data structures and is accessible by a computer. Any connection is also properly termed a computer-readable medium. For example, if software is transmitted from a web site, a control computer, or other remote source using coaxial cable, fiber optic cable, twisted pair, DSL, or wireless technologies such as infrared, radio, and microwave, then the coaxial cable, fiber optic cable, twisted pair, DSL, or wireless technologies such as infrared, radio, and microwave are included in the definition of the medium. As used herein, disk and disc include compact disc (CD), laser disc, optical disc, digital versatile disc (DVD), floppy disk, and Blu-ray disc, where disk typically reproduces data magnetically, while disc reproduces data optically with a laser. Combinations of the above should also be included within the scope of computer-readable media.

[0080] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

[0081] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit them. Although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. These modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for preparing camel milk-derived exosomes by freeze-drying, characterized in that: The following steps are included: A rennet solution is prepared and rennet emulsification is performed, cell debris is removed to collect the camel milk middle layer and the pH is adjusted to obtain an middle layer solution, the rennet solution is added to the middle layer solution for rennet incubation, and the mixed solution after incubation is centrifuged, filtered, and freeze-dried to obtain camel milk exosome freeze-dried powder.

2. The method for preparing freeze-dried camel milk-derived exosomes according to claim 1, characterized in that: The steps of preparing the rennet solution and performing rennet emulsification are as follows: dissolving 0.035 mg of rennet in 1 L of a sodium chloride solution having a mass concentration of 1% to prepare a rennet solution having a concentration of 0.035 mg / L; Place the rennet solution in a 37±0.5℃ water bath and activate for 30 minutes.

3. The method for preparing freeze-dried camel milk-derived exosomes according to claim 1 or 2, characterized in that: The step of removing cell debris to collect the middle layer of camel milk and adjusting the pH to obtain the middle layer solution is as follows: centrifuging the camel milk at 4°C and 2000r / min for 15 minutes using a refrigerated centrifuge to collect the middle layer, adding glacial acetic acid solution to the collected middle layer, and adjusting the pH value to 6.0±0.05 to obtain the middle layer solution.

4. The method for preparing freeze-dried camel milk-derived exosomes according to claim 3, characterized in that: The step of adding the chymosin solution to the middle layer solution for chymosin incubation is as follows: taking the activated chymosin solution and adding it to the middle layer solution after adjusting the pH value, and mixing them thoroughly; incubating the mixed solution in a 37±0.5°C water bath for 30-35 minutes.

5. The method for preparing freeze-dried camel milk-derived exosomes according to claim 4, characterized in that: Step 1: Add the rennet solution to the middle layer solution for rennet incubation according to the following ratio: 2.5 ml of rennet solution is added to every 50 ml of camel milk.

6. The method for preparing freeze-dried camel milk-derived exosomes according to claim 3, characterized in that: The step is to remove cell debris to collect the camel milk middle layer and adjust the pH to obtain the middle layer solution, wherein the mass concentration of the glacial acetic acid solution is 10%.

7. The method for preparing freeze-dried camel milk-derived exosomes according to any one of claims 1, 2 or 4-6, characterized in that: The step is to centrifuge, filter and freeze-dry the incubated mixed solution to obtain camel milk exosome freeze-dried powder. Specifically, at 4°C, the incubated mixed solution is centrifuged at 2000r / min for 20min to obtain a supernatant, at 4°C, the supernatant obtained by centrifugation is centrifuged at 20000r / min for 30min, and then filtered with a 2um syringe filter. The filtered supernatant is freeze-dried at 50°C and 0.35mBar, and the obtained precipitate is centrifuged at 20000r / min for 15min at 4°C to finally collect the camel milk exosome freeze-dried powder.

8. A camel milk-derived exosome, characterized in that: The extract is obtained by extracting using the freeze-drying preparation method described in any one of claims 1 to 7.

9. A computer-readable storage medium, characterized in that: The computer-readable storage medium stores computer-executable instructions, and the computer-executable instructions are used to enable a computer to execute the camel milk-derived exosome freeze-drying preparation method as described in any one of claims 1 to 7.

10. A camel milk-derived exosome freeze-drying preparation system, characterized in that: It comprises a memory, a control processor and a computer program stored in the memory and executable on the control processor, wherein the control processor executes the program to implement the freeze-dried preparation method of camel milk-derived exosomes as described in any one of claims 1 to 7.

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