Testing method of imaging equipment and fluorescent gel for testing imaging equipment
By using an adsorption combination of fluorescent gel and imaging equipment, the problem of unstable liquid fluorescent environment in gravity-free space is solved, and stable test objects and accurate test results are achieved.
Patent Information
- Application Number
- CN202510031554.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-08
- Publication Date
- 2025-05-30
AI Technical Summary
In the prior art, the liquid fluorescent environment is unstable in the gravity-free space, and the influence of gravity causes the loss of fluorescent solution, which cannot be used as a stable test object.
By using fluorescent gel as the test object and cooperating with the imaging equipment to overcome the gravity influence of the fluorescent environment itself, and avoid discreteness through its own viscous polymerization, thus forming a stable test object.
It realizes the stability of the test object in a gravity-free environment, improves the accuracy of the test results, and is suitable for testing at different angles.
Smart Images
Figure CN120063662A_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of multiphoton imaging technology, and particularly to a test method for an imaging device and a fluorescent gel for testing an imaging device. Background Art
[0002] Multiphoton imaging technology is a non-linear optical imaging technology and belongs to the category of generalized fluorescence microscopes. On the focal plane formed by the excitation light, the fluorescent material responds to the excitation light and releases fluorescent signals. After these fluorescent signals are detected, a fluorescent image at the focal plane can be formed.
[0003] In order to test whether a fluorescence microscope including a multiphoton microscopy imaging device meets the design performance, or to perform imaging comparison between different devices, or to perform regular calibration or maintenance on the devices that have left the factory, it is necessary to use the device to be tested to perform imaging tests on standard samples, and evaluate the performance of the imaging device or locate the problems of the imaging device through the imaging results. To ensure the accuracy of the test results, the standard sample should have an appropriate concentration of fluorescent substance, a fixed distribution of fluorescent substance, and be stable and reliable. Therefore, how to form a stable standard sample as the test object is a technical problem that needs to be solved urgently by those skilled in the art. Summary of the Invention
[0004] In view of this, the embodiments of this application provide a test method for an imaging device and a fluorescent gel for testing an imaging device. A stable test object is realized through the fluorescent gel to overcome the deficiencies of the prior art.
[0005] In a first aspect, the embodiments of this specification provide a test method for an imaging device. The imaging device is based on multiphoton microscopy imaging technology. The method includes: determining a fluorescent gel based on the test task of the imaging device, where the fluorescent gel is configured as a homogeneous colloid formed by a target fluorescent material and a gel medium. In response to the adsorption cooperation between the fluorescent gel and the imaging device, adjusting the focal plane position of the imaging device. When the focal plane position is at the target focusing position, determining the fluorescent test image at the target focusing position to determine a set of fluorescent test images at multiple target focusing positions, where the target focusing position corresponds to the fluorescent test image one by one. Determining the test result of the test task based on the structural characteristics of the fluorescent gel, multiple target focusing positions, and the set of fluorescent test images, where the structural characteristics at least include the uniformity characteristics of the fluorescent gel.
[0006] In a second aspect, the embodiments of this specification provide a fluorescent gel for testing an imaging device. The fluorescent gel is configured as a homogeneous colloid formed by a target fluorescent material and a gel medium. The fluorescent gel is used as a test object to be adsorbed and cooperated with the imaging device to perform the test method as in the first aspect.
[0007] The embodiments of the present application provide a method for testing an imaging device and a fluorescent gel for testing an imaging device. Through the adsorption cooperation between the fluorescent gel and the imaging device, the fluorescent gel can overcome the influence of its own gravity on the test based on the adsorption force on the imaging device, and at the same time can polymerize based on its own viscosity to avoid dispersion in a zero-gravity environment, thereby forming a relatively stable test object. In addition, the fluorescent gel itself is a well-mixed homogeneous colloid, and the concentration of the fluorescent material in the fluorescent gel is constant, improving the accuracy of the test results. Description of the Drawings
[0008] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as limiting the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without creative efforts.
[0009] Figure 1 It is an exemplary scenario diagram of the imaging device test environment provided by some embodiments of the present application.
[0010] Figure 2 It is an exemplary flowchart of the imaging device test method provided by some embodiments of the present application.
[0011] Figure 3 It is an exemplary flowchart of a method for determining a target focusing position provided by some embodiments of the present application.
[0012] Figure 4 It is an exemplary flowchart of another method for determining a target focusing position provided by some embodiments of the present application. Detailed Embodiments
[0013] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope protected by the present application.
[0014] Application Overview:
[0015] In the related art, the test object of an imaging device is generally constructed based on a fluorescent solution. For example, by means of sample preparation, a fluorescent solution can be used as a microscopic object to construct a glass slide as a test object. For another example, a container can be used to hold the fluorescent solution as a test object. Among them, the fluorescent solution can be formed by a fluorescent material and a liquid medium, and the fluorescent material can present a preset concentration in the fluorescent solution.
[0016] When performing the test, the test object and the imaging device can be assembled accordingly, and multiphoton microscopic imaging can be performed to obtain the corresponding test image. Among them, the test image can be used to determine some parameters of the imaging device. For example, the excitation rate of the imaging device to the fluorescent material and the standard brightness at the concentration can be determined according to the brightness of the test image (such as the brightness of the corresponding fluorescent signal). Therefore, in actual use, the actual concentration and distribution of the fluorescent material in the imaging object can be inferred based on the actual brightness of the fluorescent signal and the standard brightness.
[0017] As the application scenarios of imaging equipment become more complex, the liquid fluorescent environment in the above-mentioned related technologies is difficult to adapt to the imaging requirements of specific scenarios, and may be unstable, resulting in unstable test results. The above-mentioned fluorescent solution has at least the following technical problems:
[0018] First, when in a zero-gravity space (such as a space station), the shape and movement trajectory of the fluorescent solution in the zero-gravity space cannot be controlled, and it does not meet the stability requirements of the test object and cannot be used as a test object.
[0019] Second, the liquid fluorescent environment is affected by its own gravity, and the imaging device can only be set above the fluorescent solution during testing. When the liquid fluorescent environment is located above the imaging device, the liquid fluorescent environment will be lost due to its own gravity. For contact imaging devices, especially medical multiphoton imaging devices, it is often necessary to contact the imaging object at different angles, and the liquid fluorescent environment cannot be tested in this case and cannot be used as a test object.
[0020] In view of the above problems, the embodiments of the present application provide a method for testing an imaging device and a fluorescent gel for testing an imaging device. The fluorescent gel forms a fluorescent environment, and the fluorescent gel can be adsorbed with the medium component of the imaging device, thereby overcoming the gravity of the fluorescent environment itself based on the adsorption force of the adsorption, thereby forming a relatively stable test object. In addition, the fluorescent gel itself is a fully mixed uniform colloid, and the concentration of the fluorescent material in the fluorescent gel is constant, which improves the accuracy of the test results. Various non-limiting embodiments of the present application will be specifically described below with reference to the accompanying drawings.
[0021] Exemplary Application Scenarios:
[0022] Figure 1 It is an application scenario diagram of the imaging device testing process provided in some embodiments of the present application.
[0023] like Figure 1As shown, in application scenario 100 of the imaging device testing process, it may include an imaging device 110 and a fluorescent gel 120. Among them, the fluorescent gel 120 is adhesively cooperated with the imaging device 110, and the fluorescent gel 120 is used as the test object of the imaging device 110 for testing.
[0024] The imaging device 110 may be an imaging device based on multi-photon microscopy technology. Among them, the imaging device 110 can emit excitation light to the imaging object, so that the excitation light forms a focal plane that satisfies the excitation conditions of the imaging object at a preset position. The fluorescent material located in the focal plane is excited by the excitation light and emits a fluorescent signal, thereby forming a fluorescent image. Among them, the fluorescent image can reflect the distribution of the fluorescent material in the imaging object at the focal plane. Thus, the internal structure of the imaging object can be determined through the fluorescent image.
[0025] In some embodiments, the imaging device 110 may be a contact imaging device. That is, when performing multi-photon microscopy, the imaging device 110 is in contact with the imaging object, and the focal plane is adjusted to the inside of the imaging object to obtain a fluorescent image that reflects the internal structure of the imaging object.
[0026] The fluorescent gel 120 may be a homogeneous colloid of a target fluorescent material and a gel medium. Among them, the colloid may mean that the fluorescent gel 120 may be a semi-solid material with viscosity and a certain fluidity. The homogeneous colloid may mean that the distribution of the target fluorescent material in the fluorescent gel 120 is uniform, that is, within the tolerance range, the concentration of the target fluorescent material is a fixed value in the fluorescent gel 120.
[0027] The fluorescent gel 120 may be a colloid in an amorphous state and can be coated on the imaging window during use. In some embodiments, considering that the fluorescent gel provided in this application is generally used as the test environment of a two-photon microscope, its coating thickness is generally 3-5 mm.
[0028] The target fluorescent material may refer to the fluorescent material in the fluorescent material that is adapted to the imaging device 110. For example, the target fluorescent material may be a fluorescent material corresponding to the excitation light type of the imaging device 110. In some embodiments, the target fluorescent material can be determined according to the excitation light type of the imaging device 110. For example, the imaging device 110 can be realized based on non-linear laser scanning such as two-photon, three-photon, Raman, etc., and the target fluorescent material can be a fluorescent material with better imaging effect in the corresponding technology. In some embodiments, the target fluorescent material can also be determined according to the fluorescent material in the imaging object to be actually imaged subsequently. For example, the target fluorescent material may be the same as or similar to the fluorescent material in the imaging object (such as the same or similar fluorescent signal band).
[0029] The gel medium may refer to a material medium that forms a gel. In some embodiments, any semi-solid material that is light-transmissive and has viscosity and a certain fluidity may be used as the gel medium. For example, the gel medium may be a silicone gel.
[0030] In some embodiments, the gel medium can be selected or doped according to the imaging object so that the refractive index of the fluorescent gel 120 corresponds to the refractive index of the imaging object in the test task (such as being the same within the tolerance range). In this way, the imaging condition of the fluorescent gel 120 can better feedback the imaging condition in the actual test, thereby determining more accurate adjustment parameters to improve the accuracy of the imaging device.
[0031] like Figure 1 As shown, the fluorescent gel 120 can be matched with the imaging device 110 by adsorption. The fluorescent gel 120 can be used as a test object and matched with the imaging device 110 according to the matching relationship between a conventional imaging object and the imaging device 110. For example, for a contact imaging device 110, the fluorescent gel 120 can be directly applied to the imaging window of the imaging device 110 (such as applied to the outside of the imaging window 113).
[0032] In some embodiments, the fluorescent gel 120 can also be adsorbed with the imaging device 110 based on the accommodating cavity 130. The fluorescent gel 120 fills the accommodating cavity 130, thereby limiting the flow of the fluorescent gel 120 and improving the stability of the fluorescent gel 120.
[0033] In some embodiments, considering that contact imaging devices are generally based on low-light environment imaging, the housing cavity 130 can be prepared based on light-shielding materials. Among them, low-light environment imaging can refer to that the imaging device 110 does not receive external ambient light during imaging. The housing cavity prepared based on light-shielding materials can block ambient light to form a low-light environment. In some embodiments, the housing cavity 130 and the imaging device 110 can be connected based on a mechanical structure, thereby improving the stability of the fluorescent gel.
[0034] When performing the test, the imaging device 110 can release excitation light to the fluorescent gel 120 and adjust the focal plane of the excitation light so that the fluorescent material releases a fluorescent signal to form a corresponding test image, thereby determining the test result. For more information about the test process, see Figure 2 and its related contents.
[0035] In some embodiments, the imaging device 110 is constructed based on a solid-state elastic medium, and specifically may include an imaging module 111, a solid-state elastic medium 112, and an imaging window 113. Among them, the imaging module 111 is used to emit excitation light, the solid-state elastic medium 112 matches the refractive index of the imaging object to improve imaging accuracy, and the imaging window 113 is used to contact the imaging object. Thus, the focal plane of the excitation light can be adjusted by adjusting the position of the imaging module 111, so that the focal plane position is the target focusing position.
[0036] For the aforementioned imaging device 110 based on a solid-state elastic medium, it may be necessary to determine the cooperation state between the imaging module 111 and the solid-state elastic medium 112 during imaging. Only when in a preset cooperation state can the imaging device 110 image normally. Then, the present application also provides a method for determining the cooperation state between the imaging module 111 and the solid-state elastic medium 112.
[0037] Exemplary Test Methods:
[0038] Figure 2 It is an exemplary flowchart of the imaging device testing method provided by some embodiments of the present application.
[0039] In some embodiments, the imaging device testing method P200 can be executed by a processing device. For example, a processor integrated in an imaging device (such as the imaging device 110) can execute P200 according to user settings.
[0040] As Figure 2 shown, P200 may include the following steps:
[0041] S210, determine a fluorescent gel based on the test task of the imaging device.
[0042] In S210, the imaging device can be the imaging device undergoing the test (such as the imaging device 110). The fluorescent gel can be used as the test object for this test. For specific details about the imaging device and the fluorescent gel, reference can be made to Figure 1 and its related descriptions. Details will not be elaborated here.
[0043] The test task can be at least one test content to be executed in this test. In some embodiments, the test task can be characterized as a test of the performance parameters of the imaging device. For example, the test task can include tests of the excitation light transmittance and excitation rate of the imaging device.
[0044] In some embodiments, the test task can include multiple test purposes during the test process, and each test target can correspond to different performance parameters. For example, the test task can include one or more combinations of multiple test contents such as display uniformity test, imaging position test, imaging range test, and cooperation relationship test.
[0045] In some embodiments, the test task can be determined according to the test content required in practice. For example, the test content can be directly set by the user. As another example, the test content to be tested can be determined based on the imaging object for subsequent imaging. Exemplarily, parameters to be calibrated in subsequent imaging can be determined based on the imaging object, thereby determining the test task.
[0046] In some embodiments, determining the fluorescent gel based on the test task can be embodied as determining the standard concentration of the target fluorescent material in the fluorescent gel based on the test task, thereby determining the fluorescent gel. In some embodiments, the type of the target fluorescent material and / or the type of the gel medium can also be determined based on the imaging method and the imaging object in the test task. For example, the refractive index of the gel medium can be within the tolerance range consistent with the refractive index of the imaging method.
[0047] S220. In response to the adsorption fit between the fluorescent gel and the imaging device, adjust the focal plane position of the imaging device.
[0048] In some embodiments, after determining the fluorescent gel, the executor can perform the adsorption fit between the fluorescent gel and the imaging device, and then execute S220.
[0049] The adsorption fit can refer to making the fluorescent gel adhere to the imaging window of the imaging device based on the viscosity of the fluorescent gel itself, thereby realizing the fit between the imaging device and the fluorescent gel. Based on the adsorption fit between the fluorescent gel and the imaging device, the influence of its own gravity can be overcome, enabling the imaging device to perform tests at any angle. In addition, based on the adsorption fit, the fluorescent gel will not detach from the imaging device in a zero-gravity environment, thus ensuring the stability of the test object in different test scenarios.
[0050] In some embodiments, the aforementioned S220 can be executed by adjusting the position of the imaging-related device (such as the position of the imaging module). For details, reference can be made to Figure 3 、 Figure 4 and its related descriptions.
[0051] S230. When the focal plane position is at the target focusing position, determine the fluorescent test image at the target focusing position to determine the set of fluorescent test images at multiple target focusing positions.
[0052] The focusing position can refer to the position where the focal plane is located. The target focusing position can refer to the focusing position selected during the test. In some embodiments, the target focusing position can be determined according to the test task and is a focusing position that can directly or indirectly reflect the test result. For example, the test result (such as the imaging range) can be directly determined based on multiple target focusing positions. As another example, the test result (such as the display uniformity) can be determined according to the fluorescent test image corresponding to the target focusing position.
[0053] In some embodiments, the target focus position can be determined according to the test task. Among them, the target focus position can be a focus position that meets the requirements of the test task. For example, when the test task includes a display uniformity test, the test task requires imaging inside the test object (fluorescent gel), then the target focus position can include the position where the focal plane is located within the fluorescent gel, and this position can be denoted as the first focus position. For another example, when the test task includes an imaging position test of the imaging device, the test task requires detecting parameters at the position where the focal plane meets the imaging device and the fluorescent gel (such as the position parameter of the imaging module in the imaging device), and this position can be denoted as the second focus position.
[0054] The fluorescence test image can refer to the fluorescence image obtained by performing multi - photon imaging at the target focus position. In some embodiments, when testing multiple positions, multiple target focus positions and corresponding multiple fluorescence test images can be determined. Among them, the target focus position and the fluorescence test image are in one - to - one correspondence, and the multiple fluorescence test images can be denoted as a fluorescence test image set.
[0055] In some embodiments, the fluorescence test image can be determined based on the target focus position. That is, when the focal plane is focused on the target focus position, the fluorescence signal collected at this position can form the corresponding fluorescence test image. In some embodiments, the fluorescence test image can also be used as a detection index to determine the target focus position.
[0056] In some embodiments, the multi - photon microscopy imaging technology generally uses a scanning method to image at each target focus position, that is, it can scan each position in the focal plane to obtain the fluorescence signal at the corresponding position, thereby constructing a fluorescence test image. Among them, the fluorescence test image can be composed of multiple pixels, and each pixel can characterize the fluorescence signal intensity corresponding to the corresponding position in the focal plane through a gray value.
[0057] In some embodiments, the fluorescence test image is similar to the conventional imaging process of the multi - photon microscopy imaging technology. For details, reference can be made to Figure 3 and its related descriptions.
[0058] S240. Determine the test result of the test task based on the structural characteristics of the fluorescent gel, multiple target focus positions, and the fluorescence test image set.
[0059] The structural characteristics of the fluorescent gel can refer to the characteristics that the fluorescence image formed based on the aforementioned fluorescent gel should possess. Among them, the structural characteristics at least include the uniformity characteristics of the fluorescent gel. That is, the corresponding fluorescence image should be uniform (the gray value in the image is uniform).
[0060] The test result may refer to the test result of the imaging device based on the test task. Among them, the test result can generally be characterized as the performance parameters of the imaging device corresponding to the test task and / or the evaluation of the performance parameters. For example, when performing the excitation condition test, the test result may include the detected value of the excitation rate of the imaging device and whether the detected value of the excitation rate meets the imaging requirements.
[0061] In some embodiments, the test result may be performed based on whether the fluorescence test image conforms to the structural characteristics of the fluorescent gel. Among them, based on the uniformity of the aforementioned fluorescent gel, the test result may at least include whether the fluorescence signal (presented as the gray value in the image) in the fluorescence test image is evenly distributed.
[0062] In some embodiments, there may be different test requirements for different target focusing positions, that is, the position requirements and image requirements can be determined based on the test task and the structural characteristics of the fluorescent gel. For the target focusing position that meets the position requirements, the test result is determined based on the fluorescence test image corresponding to the target focusing position and the test requirements.
[0063] Among them, the image requirements include at least one of the gray value requirements, the gray value distribution requirements, the image requirements, and the gel network characteristics.
[0064] Specifically, the gray value requirement can reflect the requirement of the imaging device for the excitation rate, that is, the imaging device needs to excite the fluorescence signal of the fluorescent gel and meet certain intensity requirements.
[0065] The gray value distribution requirement can reflect the requirement for the flatness of the focal plane of the imaging device. That is, when the focal plane is not flat, the gray value distribution will be uneven at a specific focusing position, especially at the corners of the image.
[0066] The gel network characteristic requirement may refer to that the fluorescence test image needs to show the gel network of the fluorescent gel, which can reflect the resolution ability of the imaging device. Among them, there may be a macromolecular polymer structure in the fluorescent gel, and this structure can be denoted as the gel network. In a possible case, the target fluorescent material cannot enter the gel network, so that the network structure appears in the corresponding fluorescence test image. Thus, when the gel network characteristic can be resolved in the fluorescence test image, it can be determined that the resolution ability of the imaging device meets the requirements.
[0067] In some embodiments, different image requirements may be adopted for target focusing positions at different locations. In addition, the test task may include the calibration of the target focusing position. For details, see Figure 4 and its related descriptions.
[0068] In some embodiments, during the actual testing process, more complex testing tasks can also be constructed based on the aforementioned position requirements and image requirements. For example, the gel network feature requirements at different imaging positions can be tested to determine the maximum imaging range within which the gel network can be resolved by this imaging device.
[0069] In the aforementioned testing method, through the adsorption cooperation between the fluorescent gel and the imaging device, the fluorescent gel can overcome the influence of its own gravity on the test based on the adsorption force on the imaging device. At the same time, it can avoid dispersion in a zero-gravity environment based on its own viscosity polymerization, thereby forming a relatively stable test object. In addition, the fluorescent gel itself is a well-mixed homogeneous colloid, and the concentration of the fluorescent material in the fluorescent gel is constant, improving the accuracy of the test results.
[0070] A Method for Determining the Target Focus Position:
[0071] Figure 3 It is an exemplary flowchart of a method for determining a target focusing position provided by some embodiments of the present application.
[0072] In some embodiments, Figure 3 The process P300 shown can be executed by a processing device. For example, a processor integrated in an imaging device (such as imaging device 110) can execute P300 according to user settings.
[0073] As Figure 3 shown, P300 can include the following steps:
[0074] S310: Adopt a step-by-step adjustment strategy to gradually adjust the position scale of the focal plane position along the imaging direction from the initial position.
[0075] In some embodiments, the imaging device can adjust the focal plane position through the position scale. Then, during the test, similar to actual imaging, a step-by-step control method can be adopted to gradually adjust the focal plane position along the imaging direction. Among them, the specific adjustment interval can be adjusted according to actual needs. In multi-photon microscopy technology, the interval for each adjustment is generally at the μm level, and the entire range is generally at the mm level.
[0076] S320: In response to the change in the position scale, determine the candidate focusing position and the candidate fluorescence image corresponding to the current position scale to determine a set of multiple candidate focusing positions and their corresponding candidate fluorescence images.
[0077] In some embodiments, during the process of adjusting the position scale mentioned above, when the position scale changes, scanning imaging can be performed at this scale to obtain the candidate fluorescence image corresponding to the current position scale. Among them, the corresponding candidate focusing position can be represented by the corresponding position scale. The candidate focusing positions determined in this way can be in one-to-one correspondence with the candidate fluorescence images.
[0078] S330. Determine the fluorescence test image sets at and for multiple target focusing positions from multiple candidate focusing positions.
[0079] In some embodiments, tests within the range of the imaging device can be completed based on the foregoing test method. Thus, multiple candidate focusing positions are determined. And the target focusing positions and their fluorescence test images are determined from the multiple candidate focusing positions through each candidate fluorescence image or candidate focusing position, so as to determine multiple target focusing positions and their fluorescence test image sets.
[0080] In some embodiments, this application mainly relates to three types of target focusing positions, specifically the first focusing position, the second focusing position, and the third focusing position. Then the foregoing target focusing positions can be one or a combination of more than one of the first focusing position, the second focusing position, and the third focusing position.
[0081] The first focusing position may refer to the target focusing position where the focal plane position is inside the fluorescent gel. In some embodiments, the first focusing position may be the position where this application mainly conducts tests. Then during the test, the first focusing position can be determined from the multiple target focusing positions, and the first fluorescence image corresponding to the first focusing position is determined. Then, based on the first fluorescence image, the image gray-scale distribution is determined. Based on the uniformity feature and the image gray-scale distribution, the test result of the display uniformity test in the test task is determined.
[0082] In some embodiments, the first focusing position can also be tested based on the foregoing gray-scale value requirements, gel network feature requirements, etc., so as to determine the excitation rate and resolution ability of the imaging device at the first focusing position.
[0083] The second focusing position may refer to the target focusing position where the focal plane position is at the interface between the fluorescent gel and the imaging device.
[0084] In some embodiments, when the foregoing imaging device is an imaging device for a low-light environment, a step change will occur at the second focusing position. That is, before the focal plane position reaches the second focusing position, the imaging device cannot structure an external light source, so there is no fluorescence signal. When reaching the second focusing position, the focal plane formed by the excitation light of the imaging device just contacts the fluorescent gel, thus generating a fluorescence signal. Thus, before the second focusing position, there is no fluorescence signal. There is a fluorescence signal after the second focusing position. Thus, the second focusing position can be identified based on this step signal.
[0085] In some embodiments, the position scale can be converted into the depth inside the imaging object based on the second focusing position. That is, the reference position scale of the focal plane position at the second focusing position can be determined, and the reference position scale is used as the test result of the imaging reference position in the test task.
[0086] The third focusing position may refer to a target focusing position that reflects the maximum adjustment amount of the focal plane position. Among them, the maximum adjustment amount may refer to the maximum adjustment amount restricted externally, that is, the imaging device cannot further move the focal plane position along the imaging direction due to its own limitations. The maximum adjustment amount may also be the maximum test range of the test task, that is, there is no test significance beyond this range.
[0087] Based on the foregoing third focusing position, the limit position scale of the focal plane position at the third focusing position can be determined, and then based on the reference position scale and the limit position scale, the test result of the imaging range in the test task can be determined. Among them, the reference position scale and the limit position scale can be subtracted to determine the actual measurement range, and compared with the requirements of the imaging range in the test task, so as to determine the test result.
[0088] In some embodiments, based on the foregoing second focusing position and the third focusing position, the foregoing first focusing position can be determined. That is, the first focusing position is between the second focusing position and the third focusing position.
[0089] In some embodiments, considering Figure 1 the assembly situation shown, the test method provided in this application can be applied to an imaging device using a solid-state elastic medium. Based on this imaging device, the target focusing position can be determined based on the following method.
[0090] Figure 4 is an exemplary flowchart of another method for determining the target focusing position provided in some embodiments of this application.
[0091] As Figure 4 shown, P400 may include the following steps:
[0092] S410. Adjust the position scale of the imaging module to adjust the focal plane position until the imaging module is in contact fit with the solid-state elastic medium.
[0093] In some embodiments, for Figure 1 the imaging device shown, the position of its imaging module can be controlled by a displacement stage inside the imaging device. Before the test, it is necessary to control the position (i.e., the position scale) of the imaging module so that the imaging module is in contact fit with the fluorescent gel. For details, please refer to the relevant application documents of this application.
[0094] Among them, when the imaging module is in contact fit with the solid-state elastic medium, the solid-state elastic medium completely covers the light port diameter of the imaging module. That is, the excitation light released by the imaging module all passes through the solid-state elastic medium and then exits from the imaging window.
[0095] It should be noted that in practical applications, when the imaging module is in contact with the solid elastic medium, the focal plane position is generally inside the imaging device. When the focal plane position is inside the imaging device, it can be determined that the wrong solid elastic medium has been selected / the solid elastic medium is unqualified. For details, reference can be made to the related technology of this application.
[0096] S420. Adjust the focal plane position and obtain the position scale and image features of the current focusing position.
[0097] In some embodiments, the foregoing S420 is similar to the foregoing P300 and generally can be achieved by stepwise adjusting the focal plane position. Optionally, considering the specific position of the foregoing target focusing position, in S420, a complete imaging scan may not be performed, and only a local scan is executed to obtain image features.
[0098] S430. Determine a plurality of target focusing positions and a set of fluorescence test images based on the position features and / or image features.
[0099] In some embodiments, the target focusing position can be determined based on the foregoing image features and / or position features.
[0100] Specifically, in response to a step change in the image features at the current focusing position, the current focusing position is denoted as the second focusing position and configured as the target focusing position to obtain a second fluorescence image at the second focusing position.
[0101] In response to the position scale being unable to change along the depth direction at the current focusing position, the current focusing position is denoted as the third focusing position and configured as the target focusing position to obtain a third fluorescence image at the third focusing position.
[0102] In response to the position scale being between the second focusing position and the third focusing position, the current focusing position is denoted as the first focusing position and configured as the target focusing position to obtain a first fluorescence image at the first focusing position.
[0103] In some embodiments, considering that different imaging depths may affect the fluorescence image, when performing tests, the first focusing positions at different depths can be selected. That is, at least two test depth ranges can be determined according to the test task. The focusing depth of the current focusing position is determined based on the second focusing position. In response to the focusing depth being within the test depth range, the current focusing position is used as the first focusing position within the test depth range to obtain the first focusing positions and their first fluorescence images within each test depth range. Generally, two first focusing positions can be selected, one of which is close to the second focusing position and the other is close to the third focusing position.
[0104] Any combination of the above optional technical solutions can form an optional embodiment of the present application, which will not be elaborated one by one here.
[0105] Those of ordinary skill in the art can realize that the units and algorithm steps of each example described in combination with the embodiments disclosed herein can be implemented by electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are executed in a hardware or software manner depends on the specific application and design constraints of the technical solution. Professional technicians can use different methods to implement the described functions for each specific application, but such implementation should not be considered to exceed the scope of the present application.
[0106] Those skilled in the art can clearly understand that for the convenience and simplicity of description, the specific working processes of the systems, devices, and units described above can refer to the corresponding processes in the foregoing method embodiments, which will not be elaborated here.
[0107] In several embodiments provided by the present application, it should be understood that the disclosed systems, devices, and methods can be implemented in other ways. For example, the device embodiments described above are merely illustrative. For example, the division of the units is only a logical function division. In actual implementation, there may be other division methods. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the displayed or discussed couplings or direct couplings or communication connections to each other can be through some interfaces. The indirect couplings or communication connections of the devices or units can be in electrical, mechanical, or other forms.
[0108] The units described as separate components may or may not be physically separated. The components displayed as units may or may not be physical units, that is, they can be located in one place, or distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment.
[0109] In addition, the functional units in each embodiment of the present application can be integrated into one processing unit, or each unit can exist physically alone, or two or more units can be integrated into one unit.
[0110] If the above-mentioned functions are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art or a part of this technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods described in various embodiments of this application. The aforementioned storage medium includes: various media such as USB flash drives, mobile hard disks, read-only memories (ROM, Read-Only Memory), random access memories (RAM, Random Access Memory), magnetic disks, or optical discs that can store program check codes.
[0111] It should be noted that in the description of this application, the terms "first", "second", "third", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In addition, in the description of this application, unless otherwise specified, the meaning of "a plurality of" is two or more.
[0112] The above are only the preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent replacements, etc. made within the spirit and principle of this application shall be included within the protection scope of this application.
Claims
1. A method for testing an imaging device, characterized in that: The imaging device is based on multiphoton microscopy technology, and the method comprises: determining a fluorescent gel based on a test task of the imaging device, wherein the fluorescent gel is configured as a uniform colloid formed by a target fluorescent material and a gel medium; In response to the fluorescent gel being adsorbed and mated with the imaging device, adjusting the focal plane position of the imaging device; When the focal plane position is located at a target focus position, determining a fluorescence test image at the target focus position to determine a set of fluorescence test images at multiple target focus positions, wherein the target focus positions correspond to the fluorescence test images one by one; The test result of the test task is determined based on the structural features of the fluorescent gel, the multiple target focus positions, and the set of fluorescent test images, wherein the structural features at least include uniformity features of the fluorescent gel.
2. The testing method according to claim 1, characterized in that: The determining of the test result of the test task based on the structural features of the fluorescent gel, the target focus position and the fluorescent test image set includes: Determine a first focus position from the multiple target focus positions, and determine a first fluorescent image corresponding to the first focus position, wherein a focal plane position of the first focus position is inside the fluorescent gel; determining image grayscale distribution based on the first fluorescent image; Based on the uniformity feature and the grayscale distribution of the image, a test result of the display uniformity test in the test task is determined.
3. The testing method according to claim 1, characterized in that: The determining of the test result of the test task based on the structural features of the fluorescent gel, the target focus position and the fluorescent test image set includes: Determine a second focus position from the plurality of target focus positions, and determine a reference position scale of the focal plane position at the second focus position, wherein the focal plane position of the second focus position is where the fluorescent gel and the imaging device cooperate; The reference position scale is used as a test result of the imaging reference position in the test task.
4. The testing method according to claim 3, characterized in that: The determining of the test result of the test task based on the structural features of the fluorescent gel, the target focus position and the set of fluorescent test images includes: Determining a third focus position from the plurality of target focus positions based on the reference position scale, and determining a limit position scale of the focal plane position at the third focus position, wherein the third focus position reflects a maximum adjustment amount of the focal plane position; A test result of the imaging range in the test task is determined based on the reference position scale and the limit position scale.
5. The testing method according to any one of claims 2 to 4, characterized in that: When the focal plane position is located at a target focus position, determining a fluorescence test image at the target focus position to determine a set of fluorescence test images at multiple target focus positions, comprises: Adopting a step-by-step adjustment strategy, the position scale of the focal plane position is gradually adjusted from the initial position along the imaging direction; In response to the position scale change, determining a candidate focus position and a candidate fluorescence image corresponding to the current position scale to determine a plurality of candidate focus positions and a set of candidate fluorescence images corresponding thereto, wherein the candidate focus positions correspond to the candidate fluorescence images one by one; The plurality of target focus positions and the set of fluorescence test images thereof are determined from the plurality of candidate focus positions, wherein the plurality of target focus positions include one or more combinations of a first focus position, a second focus position and a third focus position.
6. The testing method according to claim 1, characterized in that: The imaging device includes an imaging module, a solid elastic medium, and an imaging window. When the focal plane is located at a target focus position, before determining a fluorescence test image at the target focus position to determine a set of fluorescence test images at multiple target focus positions, the method further includes: The position scale of the imaging module is adjusted to adjust the focal plane position until the imaging module is in contact with the solid elastic medium, wherein when the imaging module is in contact with the solid elastic medium, the solid elastic medium completely covers the optical path aperture of the imaging module.
7. The testing method according to claim 6, characterized in that: When the focal plane position is located at a target focus position, determining a fluorescence test image at the target focus position to determine a set of fluorescence test images at multiple target focus positions, comprises: Adjusting the focal plane position and obtaining the position scale and image features of the current focal position; Determine the plurality of target focus positions and the set of fluorescent test images based on the position feature and / or the image feature; wherein, in response to a stepwise change of the image feature at the current focus position, the current focus position is recorded as a second focus position and configured as the target focus position, so as to acquire a second fluorescent image at the second focus position; In response to the position scale being unable to change along the depth direction at the current focus position, recording the current focus position as a third focus position and configuring it as the target focus position, so as to acquire a third fluorescent image at the third focus position; In response to the position scale being between the second focus position and the third focus position, the current focus position is recorded as the first focus position and configured as the target focus position to acquire a first fluorescent image at the first focus position.
8. The testing method according to claim 7, characterized in that: In response to the position scale being between the second focus position and the third focus position, recording the current focus position as the first focus position and configuring it as the target focus position to acquire a first fluorescent image at the first focus position, comprising: Determining at least two test depth ranges according to the test task; determining a focus depth of a current focus position based on the second focus position; In response to the focus depth being within the test depth range, a current focus position is used as a first focus position within the test depth range to obtain a first focus position within each of the test depth ranges and a first fluorescent image thereof.
9. The testing method according to claim 1, characterized in that: The determining of the test result of the test task based on the structural features of the fluorescent gel, the multiple target focus positions and the set of fluorescent test images includes: Determining position requirements and image requirements based on the test task and the structural characteristics of the fluorescent gel, wherein the image requirements include at least one of gray value requirements, gray value distribution requirements, and gel network feature requirements; For the target focus position that meets the position requirement, the test result is determined based on the fluorescent test image corresponding to the target focus position and the test requirement.
10. A fluorescent gel for testing imaging equipment, characterized in that: The fluorescent gel is configured as a uniform colloid formed by a target fluorescent material and a gel medium, and the fluorescent gel is used as a test object to be adsorbed and matched with an imaging device to perform the test method according to any one of claims 1 to 9.