Blood sample analyzer and detection method thereof

Abstract

The invention discloses a blood sample analyzer and a detection method thereof.In the blood sample analyzer, a control unit is used for measuring parameters of red blood cells / platelets of a sample through an impedance detection unit to obtain an impedance measurement value; measuring parameters of red blood cells / platelets of the sample through a forward scattering channel and a medium-angle scattering channel of the optical detection unit to obtain an optical measurement value, the scattering angle of scattered light of the forward scattering channel being 1-12 degrees, and the scattering angle of scattered light of the medium-angle scattering channel being 5-20 degrees; based on the impedance measurement and the optical measurement, an actual measurement of a parameter of the red blood cells / platelets of the sample is obtained. The blood sample analyzer does not need to carry out fluorescent staining on the sample when carrying out optical detection on the sample, the cost is relatively low, the impedance measurement value and the optical measurement value of the parameters of red blood cells / platelets are mutually corrected / supplemented, and the accuracy of parameter measurement is improved.

Description

Technical Field

[0001] This application relates to the field of in vitro diagnostic technologies, and particularly to a blood sample analyzer and its detection method. Background Art

[0002] A blood sample analyzer is an instrument that can detect cells in blood. For example, a blood sample analyzer can classify and count cells such as white blood cells, red blood cells, platelets, nucleated red blood cells, and reticulocytes in blood.

[0003] When a blood sample analyzer detects a blood sample, there may be interference between small red blood cell particles and large platelet particles. To distinguish between small red blood cell particles and large platelet particles, in related technologies, fluorescent dyes are usually used to stain cells. However, the fluorescent dye solution has high requirements for the instrument and cost. Summary of the Invention

[0004] This application provides a blood sample analyzer and its detection method to solve the technical problem in the prior art that, to distinguish between small red blood cell particles and large platelet particles, fluorescent dyes are usually used to stain cells, but the fluorescent dye solution has high requirements for the instrument and cost.

[0005] To solve the above technical problem, a technical solution adopted by this application is: to provide a blood sample analyzer, which includes: an impedance detection unit, an optical detection unit, and a control unit. Among them, the impedance detection unit is used to perform impedance detection on the sample; the optical detection unit includes at least a forward scatter channel and a mid-angle scatter channel; the control unit is connected to the impedance detection unit and the optical detection unit, and the control unit is used to: measure the red blood cell / platelet parameters of the sample through the impedance detection unit to obtain an impedance measurement value; measure the red blood cell / platelet parameters of the sample through the forward scatter channel and the mid-angle scatter channel of the optical detection unit to obtain an optical measurement value, where the scattering angle of the scattered light in the forward scatter channel is 1 to 12 degrees, and the scattering angle of the scattered light in the mid-angle scatter channel is 5 to 20 degrees; based on the impedance measurement value and the optical measurement value, obtain the actual measurement value of the red blood cell / platelet parameters of the sample.

[0006] Further, the red blood cell / platelet parameters of the sample include: the number of red blood cell / platelet particles, and the control unit is used to: correct the optical measurement value of the number of red blood cell / platelet particles by the impedance detection value of the number of red blood cell / platelet particles to obtain the actual measurement value of the number of red blood cell / platelet particles.

[0007] Further, the control unit is further configured to: identify the red blood cell particles and platelet particles in the sample according to the specificities of the red blood cell particles and platelet particles under the scattered light in the forward scatter channel and the medium angle scatter channel.

[0008] Further, the control unit is further configured to: when it is confirmed that the deviation between the impedance measurement value of the number of red blood cell / platelet particles and the optical measurement value of the number of red blood cell / platelet particles is greater than a first preset deviation, use the optical measurement value of the number of red blood cell / platelet particles as the actual measurement value of the number of red blood cell / platelet particles.

[0009] Further, the parameters of the red blood cells / platelets in the sample include: the special parameters of the red blood cells / platelets, where the special parameters of the red blood cells / platelets include at least one of the mean corpuscular volume, the number of hypochromic red blood cells, the number of hyperchromic red blood cells, and the mean platelet volume. The control unit is configured to: correct the optical measurement value of the special parameters of the red blood cells / platelets through the impedance measurement value of the special parameters of the red blood cells / platelets to obtain the actual measurement value of the special parameters of the red blood cells / platelets.

[0010] Further, the control unit is further configured to: when it is confirmed that the deviation between the impedance measurement value of the special parameters of the red blood cells / platelets and the optical measurement value of the special parameters of the red blood cells / platelets is greater than a second preset deviation, use the impedance measurement value of the special parameters of the red blood cells / platelets as the actual measurement value of the special parameters of the red blood cells / platelets.

[0011] Further, the parameters of the red blood cells / platelets in the sample include: the number of platelet particles. The control unit is further configured to: measure the number of platelet particles in the sample through the impedance detection unit to obtain the impedance measurement value of the number of platelet particles with a volume less than a preset value; measure the number of platelet particles in the sample through the forward scatter channel and the medium angle scatter channel in the optical detection unit to obtain the optical measurement value of the number of platelet particles with a volume greater than or equal to the preset value; based on the impedance measurement value of the number of platelet particles with a volume less than the preset value and the optical measurement value of the number of platelet particles with a volume greater than or equal to the preset value, obtain the actual measurement value of the number of platelet particles in the sample.

[0012] Further, the range of the preset value is: 9fL - 11fL.

[0013] Further, the optical detection unit further includes: a high angle scatter channel and a fluorescence channel, and the range of the scattering angle of the scattered light in the high angle scatter channel and the fluorescence channel is 60 - 120 degrees.

[0014] To solve the above technical problems, another technical solution adopted in this application is: to provide a detection method for a blood sample analyzer. Based on the blood sample analyzer in any of the above embodiments, the detection method includes: measuring the parameters of red blood cells / platelets in the sample through an impedance detection unit to obtain an impedance measurement value; measuring the parameters of red blood cells / platelets in the sample through the forward scatter channel and the medium angle scatter channel of the optical detection unit to obtain an optical measurement value, where the scattering angle of the scattered light in the forward scatter channel is 1 to 12 degrees, and the scattering angle of the scattered light in the medium angle scatter channel is 5 to 20 degrees; based on the impedance measurement value and the optical measurement value, obtaining the actual measurement value of the parameters of red blood cells / platelets in the sample.

[0015] Advantages of this application: Different from the prior art, the blood sample analyzer in this application includes: an impedance detection unit, an optical detection unit, and a control unit. The control unit is used for: measuring the parameters of red blood cells / platelets in the sample through the impedance detection unit to obtain an impedance measurement value; measuring the parameters of red blood cells / platelets in the sample through the forward scatter channel and the medium angle scatter channel of the optical detection unit to obtain an optical measurement value; based on the impedance measurement value and the optical measurement value, obtaining the actual measurement value of the parameters of red blood cells / platelets in the sample. When the blood sample analyzer in this application detects the sample, it does not need to stain the cells in the sample, has low requirements for the instrument, and saves costs; by measuring the parameters of red blood cells / platelets in the sample through the forward scatter channel and the medium angle scatter channel, it can better distinguish the particles of red blood cells and platelets in the sample and improve the accuracy of optical detection; in addition, the impedance detection value and the optical measurement value correct / supplement each other, which can improve the accuracy of measuring the parameters of red blood cells / platelets. Description of the Drawings

[0016] In order to more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments of this application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0017] Figure 1 It is a schematic framework diagram of an embodiment of a blood sample analyzer provided by this application;

[0018] Figure 2 is Figure 1 a schematic structural diagram of an embodiment of the optical detection unit shown;

[0019] Figure 3 It is a schematic flow diagram of an embodiment of a detection method for a blood sample analyzer provided by this application;

[0020] Figure 4 It is a schematic diagram of the distribution curve of the scattered light intensity of red blood cell and platelet particles at different angles;

[0021] Figure 5 It is a schematic diagram of the particle distribution of blood cells and platelets in the forward scattering channel and the medium angle scattering channel;

[0022] Figure 6 It is a schematic diagram of the curve of the number of platelet particles with a volume greater than or equal to 10 fL detected by the optical detection unit;

[0023] Figure 7 It is a schematic diagram of the curve of the number of platelet particles with a volume less than 10 fL detected by the impedance detection unit;

[0024] Figure 8 It is a schematic diagram of the curve of the actual measured value of the number of platelet particles by the blood sample analyzer. Detailed implementation manners

[0025] To make the above objects, features, and advantages of the present application more obvious and understandable, the following will describe the detailed implementation manners of the present application in conjunction with the accompanying drawings. It can be understood that the specific embodiments described herein are only used to explain the present application, rather than limiting the present application. Additionally, it should be noted that for the sake of description, only the parts related to the present application are shown in the accompanying drawings rather than all the structures. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present application.

[0026] The terms "first", "second", etc. in the present application are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units is not limited to the listed steps or units, but optionally further includes steps or units not listed, or optionally further includes other steps or units inherent to these processes, methods, products, or devices.

[0027] Referring to "embodiments" in this context means that the specific features, structures, or characteristics described in connection with the embodiments can be included in at least one embodiment of the present application. The phrase appears in various places in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0028] The present application first provides a blood sample analyzer. When detecting a blood sample, the blood sample analyzer can effectively distinguish the particles of microerythrocytes and the particles of large platelets without staining the cells in the blood sample. The detection method is simple and the cost is relatively low. In addition, by retaining the optical and impedance detection channels and performing relevant correction / supplementation on the detection data of the two detection channels, the blood sample analyzer of the present application can improve the accuracy of sample parameter detection.

[0029] Please refer to Figure 1 as shown Figure 1 FIG. is a schematic structural diagram of an embodiment of the blood sample analyzer provided by the present application. Specifically, the blood sample analyzer 10 includes: an impedance detection unit 11, an optical detection unit 12, and a control unit 13.

[0030] The impedance detection unit 11 includes an impedance detection channel, and the impedance detection unit 11 performs impedance detection on the sample through this impedance detection channel. The impedance detection unit 11 passes cells through a gemstone hole of a specific size in sequence. When the cells pass through the gemstone hole, it causes a change in the impedance parameter of the gemstone hole, and then the characteristics of the cells are identified. The structure of the impedance detection unit 11 is within the scope that can be understood by those skilled in the art and will not be elaborated here.

[0031] The impedance detection unit 11 can at least perform parameter tests on red blood cells and platelets in the sample. For example, the impedance detection unit 11 can be used to count the particles of red blood cells and platelets in the sample to obtain the number of particles of red blood cells and platelets. The impedance detection unit 11 can also be used to detect special parameters such as mean corpuscular volume, mean platelet volume, the number of hyperchromic red blood cells, and the number of hypochromic red blood cells. Further, the impedance detection unit 11 can also be used to perform parameter tests on white blood cells in the sample.

[0032] The optical detection unit 12 is used to perform optical detection on the sample. As Figure 2 shown Figure 2 is Figure 1 a schematic structural diagram of an embodiment of the optical detection unit shown. In this embodiment, the optical detection unit 12 includes a forward light component 121, a forward scatter component 122, a medium-angle side scatter component 123, a high-angle side scatter component 124, and a side fluorescence component 125. Among them, the forward light component 121 and the forward scatter component 122 form a forward scatter channel, the forward light component 121 and the medium-angle side scatter component 123 form a medium-angle scatter channel, the forward light component 121 and the high-angle side scatter component 124 form a high-angle scatter channel, and the forward light component 121 and the side fluorescence component 125 form a fluorescence channel. That is, Figure 2In the illustrated embodiment, the optical detection unit 12 includes four optical detection channels. In other embodiments, the optical detection unit 12 may further include other optical detection channels. It can be understood that in other embodiments, the optical detection unit 12 may also include only: a forward scatter channel and a medium angle scatter channel, which can be specifically selected and set according to the actual detection items.

[0033] Specifically, the forward scatter channel can reflect the size information of cells. The medium angle scatter channel can characterize the internal fine structure and particulate matter of cells through the conversion of Mie scattering. The high angle scatter channel characterizes the complexity of the internal structure of cells. The fluorescence channel can reflect the content of substances such as DNA (DeoxyriboNucleic Acid) and RNA (Ribonucleic Acid) in cells that can be stained by fluorescent dyes.

[0034] Further, in the present application, the scattering angle of the scattered light in the forward scatter channel is 1 to 12 degrees. In the optical detection unit 12 with higher requirements, the scattering angle of the scattered light in the forward scatter channel can be 2 to 3 degrees. The scattering angle of the scattered light in the medium angle scatter channel is 5 to 20 degrees. The scattering angle of the scattered light in the high angle scatter channel is 60 to 120 degrees. The scattering angle of the scattered light in the fluorescence channel is generally 60 to 120 degrees. The fluorescence channel and the high angle scatter channel can be beam signals of the same angle, and this beam signal is subjected to characteristic screening of wavelength or other characteristics through an optical processing device.

[0035] The control unit 13 is connected to the impedance detection unit 11 and the optical detection unit 12. The control unit 13 can control the impedance detection unit 11 to perform impedance detection on the sample, and the control unit 13 can control the optical detection unit 12 to perform optical detection on the sample. The blood sample analyzer 10 in the present application is provided with both the impedance detection unit 11 and the optical detection unit 12 at the same time, so that the detection results of the two can be mutually corrected / supplemented, thereby improving the accuracy of sample detection of the blood sample analyzer 10.

[0036] Based on the blood sample analyzer 10 of any of the above embodiments, the present application provides a detection method for a blood sample analyzer. Please refer to Figure 3 as shown, the detection method includes:

[0037] S11: Measuring the parameters of red blood cells / platelets in the sample through the impedance detection unit to obtain an impedance measurement value.

[0038] The control unit 13 controls the impedance detection unit 11 to perform impedance detection on the parameters of red blood cells / platelets in the sample to obtain an impedance measurement value of the parameters of red blood cells / platelets in the sample.

[0039] Among them, the parameters of red blood cells / platelets in the sample may include: the number of red blood cell particles, the number of platelet particles, and the special parameters of red blood cells / platelets. The special parameters of red blood cells / platelets include at least one of the following: mean corpuscular volume, mean platelet volume, the number of hyperchromic red blood cells, and the number of hypochromic red blood cells.

[0040] For example, the control unit 13 can control the impedance detection unit 11 to detect the number of red blood cell / platelet particles to obtain the impedance measurement value of the number of red blood cell / platelet particles. The control unit 13 can also detect the mean corpuscular volume through the impedance detection unit 11 to obtain the impedance measurement value of the mean corpuscular volume of the sample.

[0041] The blood sample analyzer 10 of the present application retains the impedance method for detecting cell parameters and can be used to make up for the test errors caused by the cell's own morphology in optical detection.

[0042] S12: Measure the parameters of red blood cells / platelets in the sample through the forward scatter channel and the medium angle scatter channel of the optical detection unit to obtain optical measurement values. Among them, the scattering angle of the scattered light in the forward scatter channel is 1 to 12 degrees, and the scattering angle of the scattered light in the medium angle scatter channel is 5 to 20 degrees.

[0043] The control unit 13 controls the optical detection unit 12 to measure the parameters of red blood cells / platelets in the sample through the forward scatter channel and the medium angle scatter channel therein to obtain the optical measurement values of the parameters of red blood cells / platelets in the sample.

[0044] Furthermore, before the optical detection of red blood cells in the sample, spheroidization treatment with a special reagent can be performed to improve the accuracy of optical detection.

[0045] For example, the control unit 13 can control the optical detection unit 12 to detect the number of red blood cell / platelet particles to obtain the optical measurement value of the number of red blood cell / platelet particles. The control unit 13 can also detect the cells through the optical detection unit 12 to obtain the optical measurement value of the mean corpuscular volume of the sample.

[0046] In the present application, the blood sample analyzer 10 mainly detects the parameters of red blood cells and platelets through the forward scatter channel and the medium angle scatter channel. In the optical detection method of blood samples, the particles of small-volume red blood cells and platelets both belong to small-volume blood particles, and their scattered light signals on the optical detection unit 12 are weak. Such as Figure 4As shown, the scattered light intensity is mainly concentrated in the range of 0 to 15 degrees. In the traditional optical detection scheme, the high-angle scattering channel of 60 degrees to 120 degrees is used to detect cell particles to better reflect the signal of the internal complexity of the particles. However, for the differentiation of small-volume red blood cells and platelets, it has the characteristics of low signal amplitude and being not easy to distinguish. That is, the existing methodology uses the forward and 90-degree side-scattered signals to detect particles, and the particle signals overlap, making it impossible to distinguish the particles of small red blood cells and platelets.

[0047] In this application, a medium-angle scattering channel of 5 to 20 degrees is added, and the number of red blood cell / platelet particles is detected through the forward scattering channel and the medium-angle scattering channel. The particles of small red blood cells and large platelets can be specifically identified by the scattered light of the red blood cell and platelet particles under the forward scattering channel and the medium-angle scattering channel. Figure 5 It can be seen that there is an obvious signal angle between the particles of red blood cells and platelets in the forward and medium angles, and the particle groups do not overlap. Therefore, it is possible to effectively distinguish the particles of small red blood cells and large platelets.

[0048] Therefore, in this application, by using the forward scattering channel and the medium-angle scattering channel to optically detect the number of red blood cell / platelet particles, a relatively accurate optical measurement value can be obtained.

[0049] S13: Based on the impedance measurement value and the optical measurement value, obtain the actual measurement value of the red blood cell / platelet parameters of the sample.

[0050] After obtaining the impedance measurement value and the optical measurement value of the red blood cell / platelet parameters, the control unit 13 complements / corrects the above impedance measurement value and optical measurement value to obtain the actual measurement value of the red blood cell / platelet parameters of the sample.

[0051] In the above embodiment, when the blood sample analyzer 10 detects the sample, it is not necessary to stain the cells in the sample. The detection method is simple and the cost is low. The optical detection unit 12 detects the number of cell particles through the forward scattering channel and the medium-angle scattering channel, which can effectively distinguish the particles of small red blood cells and large platelets and improve the accuracy of optical detection. Moreover, by retaining the optical detection channel and the impedance detection channel to respectively perform optical detection and impedance detection on the sample, and mutually correcting / supplementing the measurement values of the two detection methods, the accuracy of the sample detection of the blood sample analyzer 10 can be improved.

[0052] In this application, the control unit 13 can correct the impedance detection value of the particle number of red blood cells / platelets based on the optical measurement value of the particle number of red blood cells / platelets in the sample to obtain the actual measurement value of the particle number of red blood cells / platelets. The control unit 13 can correct the optical measurement value of the special parameter of red blood cells / platelets based on the impedance measurement value of the special parameter of red blood cells / platelets to obtain the actual measurement value of the special parameter of red blood cells / platelets. For example, the control unit 13 corrects the optical measurement value of the mean corpuscular volume of the sample based on the impedance measurement value of the mean corpuscular volume of the sample to obtain the actual measurement value of the mean corpuscular volume of the sample.

[0053] Specifically, when it is confirmed that the deviation between the impedance measurement value and the optical measurement value of the particle number of red blood cells / platelets in the sample is greater than the first preset deviation, the optical measurement value of the particle number of red blood cells / platelets is used as the actual measurement value of the particle number of red blood cells / platelets. The first preset deviation can be 10%, 8%, 12%, etc., and can be specifically set according to actual needs.

[0054] For example, when the optical detection value of the particle number of red blood cells in the sample is A1 and the impedance measurement value is A2, if the deviation between the two is more than 10%, the actual measurement value of the particle number of red blood cells in the sample can be taken as A1. When the optical detection value of the particle number of platelets is B1 and the impedance measurement value is B2, if the deviation between the two is more than 10%, the actual measurement value of the particle number of platelets can be taken as B1.

[0055] In this application, the optical detection unit 12 uses the medium-angle scattering channel and the forward scattering channel to detect the cell particle number, and can effectively distinguish between two particle cells, red blood cells and platelets. The optical measurement value is relatively accurate. Therefore, using the optical measurement value obtained by the optical detection unit 12 as the actual measurement value of the particle number of red blood cells / platelets has high accuracy. Therefore, the optical detection channel can be used to correct the influence of small red blood cell particles on large platelet particles in the impedance detection channel.

[0056] Furthermore, when the control unit 13 confirms that the deviation between the impedance measurement value and the optical measurement value of the special parameter of red blood cells / platelets in the sample is greater than the second preset deviation, the impedance measurement value of the special parameter of red blood cells / platelets is used as the actual measurement value of the special parameter of red blood cells / platelets. The second preset deviation can be 10%, 8%, 12%, etc., and can be specifically set according to actual needs.

[0057] For example, when the optical measurement value of the mean corpuscular volume of the sample is C1 and the impedance measurement value is C2, if the deviation between the two is more than 10%, the actual measurement value of the mean corpuscular volume of the sample can be taken as C2.

[0058] When measuring the mean corpuscular volume of a sample using an optical detection channel, affected by the morphology of red blood cells, the accuracy of the obtained optical measurement value is not high. At this time, using the impedance measurement value obtained by the impedance detection unit 11 to correct the optical measurement value can improve the accuracy of the actual measurement value of the mean corpuscular volume of the sample.

[0059] In the method for detecting the particle number of platelets, the detection principle of the impedance method is to detect the magnitude of the electrical impedance when blood cells pass through a small hole, separate platelets from other blood cells, and achieve the classification and counting of platelets. When the blood contains particles (small red blood cells or red blood cell fragments) with a volume similar to that of platelets, the impedance method will be interfered. For the optical detection channel, in the non-hemolysis scheme, the medium-angle scattering channel is used to detect platelet particles. Since the particles of small platelets are easily affected by blood shadow fragments, interference occurs. Therefore, in some other embodiments, to achieve accurate platelet counting, through the medium-angle scattering channel, accurate counting of large-volume platelets is achieved, and this optical measurement value is combined with the result of counting small-volume platelets in the impedance channel, thereby achieving high-accuracy counting of platelet particles.

[0060] Specifically, the control unit 13 measures the particle number of platelets in the sample through the impedance detection unit 11 to obtain the impedance measurement value of the particle number of platelets with a volume less than a preset value; measures the particle number of platelets in the sample through the forward scattering channel and the medium-angle scattering channel in the optical detection unit 12 to obtain the optical measurement value of the particle number of platelets with a volume greater than or equal to the preset value; based on the impedance measurement value of the particle number of platelets with a volume less than the preset value and the optical measurement value of the particle number of platelets with a volume greater than or equal to the preset value, the actual measurement value of the particle number of platelets in the sample is obtained. Among them, the range of the preset value can be: 9fL - 11fL. For example, the preset value can be 9fL, 10fL, or 11fL, etc. In this way, the impedance detection channel and the optical detection channel can more accurately detect the particle number of platelets within their respective volume ranges.

[0061] For example, as Figures 6 - 8 shown, the optical detection unit 12 and the impedance detection unit 11 perform platelet (platelet, PLT) detection. Taking the platelet volume of 10fL as the critical point, when the platelet volume is greater than 10fL, the optical measurement value of the particle number of platelets in the optical detection channel is used; when the platelet volume is less than or equal to 10fL, the impedance measurement value of the particle number of platelets in the impedance detection channel is taken. The data of the two detection channels are recombined to form a count value of the particle number of platelets with higher accuracy.

[0062] When the blood sample analyzer 10 in this application detects a sample, it does not need to stain the cells in the sample, has lower requirements for the instrument, and saves costs; the optical detection unit 12 measures the parameters of red blood cells / platelets in the sample through the forward scattering channel and the medium angle scattering channel, and can better distinguish the particles of red blood cells and platelets in the sample, improving the accuracy of optical detection; in addition, the impedance detection value and the optical measurement value of the parameters of red blood cells / platelets in the sample are corrected / supplemented with each other, which can improve the accuracy of the measurement of the parameters of red blood cells / platelets.

[0063] The above are only the implementation manners of this application, and do not limit the patent scope of this application accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of this application, or directly or indirectly applied in other related technical fields, shall be similarly included in the patent protection scope of this application.

Claims

1. A blood sample analyzer, characterized in that, the blood sample analyzer includes: an impedance detection unit for performing impedance detection on a sample; an optical detection unit including at least a forward scatter channel and a medium angle scatter channel; a control unit connected to the impedance detection unit and the optical detection unit, and the control unit is configured to: measure the parameters of red blood cells / platelets in the sample through the impedance detection unit to obtain an impedance measurement value; measure the parameters of red blood cells / platelets in the sample through the forward scatter channel and the medium angle scatter channel of the optical detection unit to obtain an optical measurement value, wherein the scattering angle of the scattered light in the forward scatter channel is 1 to 12 degrees, and the scattering angle of the scattered light in the medium angle scatter channel is 5 to 20 degrees; obtain an actual measurement value of the parameters of red blood cells / platelets in the sample based on the impedance measurement value and the optical measurement value.

2. The blood sample analyzer according to claim 1, characterized in that, the parameters of red blood cells / platelets in the sample include: the number of red blood cell / platelet particles, and the control unit is configured to: correct the impedance detection value of the number of red blood cell / platelet particles by the optical measurement value of the number of red blood cell / platelet particles to obtain an actual measurement value of the number of red blood cell / platelet particles.

3. The blood sample analyzer according to claim 2, characterized in that, the control unit is further configured to: identify the red blood cell particles and platelet particles in the sample according to the specificities of the red blood cell particles and the platelet particles under the scattered light in the forward scatter channel and the medium angle scatter channel.

4. The blood sample analyzer according to claim 2, characterized in that, the control unit is further configured to: when it is confirmed that the deviation between the impedance measurement value of the number of red blood cell / platelet particles and the optical measurement value of the number of red blood cell / platelet particles is greater than a first preset deviation, use the optical measurement value of the number of red blood cell / platelet particles as the actual measurement value of the number of red blood cell / platelet particles.

5. The blood sample analyzer according to claim 1, characterized in that, the parameters of red blood cells / platelets in the sample include: special parameters of red blood cells / platelets, wherein the special parameters of red blood cells / platelets include at least one of mean corpuscular volume, the number of hypochromic red blood cells, the number of hyperchromic red blood cells, and mean platelet volume, and the control unit is configured to: correct the optical measurement value of the special parameters of red blood cells / platelets by the impedance measurement value of the special parameters of red blood cells / platelets to obtain an actual measurement value of the special parameters of red blood cells / platelets.

6. The blood sample analyzer according to claim 5, characterized in that, The control unit is further configured to: when it is confirmed that the deviation between the impedance measurement value of the special parameter of the red blood cells / platelets and the optical measurement value of the special parameter of the red blood cells / platelets is greater than a second preset deviation, use the impedance measurement value of the special parameter of the red blood cells / platelets as the actual measurement value of the special parameter of the red blood cells / platelets.

7. The blood sample analyzer according to claim 1, wherein, the parameters of the red blood cells / platelets in the sample include: the particle number of platelets, and the control unit is further configured to: measure the particle number of platelets in the sample through the impedance detection unit to obtain an impedance measurement value of the particle number of platelets with a volume less than a preset value; measure the particle number of platelets in the sample through the forward scatter channel and the medium angle scatter channel in the optical detection unit to obtain an optical measurement value of the particle number of platelets with a volume greater than or equal to the preset value; obtain an actual measurement value of the particle number of platelets in the sample based on the impedance measurement value of the particle number of platelets with a volume less than the preset value and the optical measurement value of the particle number of platelets with a volume greater than or equal to the preset value.

8. The blood sample analyzer according to claim 7, wherein, the range of the preset value is: 9 fL - 11 fL.

9. The blood sample analyzer according to claim 1, wherein, the optical detection unit further includes: a high angle scatter channel and a fluorescence channel, and the range of the scattering angle of the scattered light in the high angle scatter channel and the fluorescence channel is 60 to 120 degrees.

10. A detection method for a blood sample analyzer, wherein, based on the blood sample analyzer according to any one of claims 1-9, the detection method includes: measuring the parameters of the red blood cells / platelets in the sample through the impedance detection unit to obtain an impedance measurement value; measuring the parameters of the red blood cells / platelets in the sample through the forward scatter channel and the medium angle scatter channel of the optical detection unit to obtain an optical measurement value, wherein the scattering angle of the scattered light in the forward scatter channel is 1 to 12 degrees, and the scattering angle of the scattered light in the medium angle scatter channel is 5 to 20 degrees; obtaining an actual measurement value of the parameters of the red blood cells / platelets in the sample based on the impedance measurement value and the optical measurement value.

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