Lipid-like wrapping system as well as preparation method and application thereof

Abstract

The invention provides a lipid-like wrapping system as well as a preparation method and application thereof. The lipid-like coating system is prepared from the following components in percentage by weight: 0.1000 to 0.5000 percent of sodium hyaluronate, 2.0000 to 6.0000 percent of glycerol, 0.5000 to 2.0000 percent of butanediol, 0.1000 to 1.0000 percent of ammonium acryloyldimethyl taurate / VP copolymer, 0.0100 to 0.1000 percent of tremella sporocarp extract, 0.0100 to 0.2000 percent of ceramide NP, 1.0000 to 3.0000 percent of coconut oil alcohol-octoate / caprate, 0.5000 to 3.0000 percent of caprylic acid / capric triglyceride, 3.0000 to 5.0000 percent of simmondsia chinensis seed oil, 1.0000 to 5.0000 percent of butyrospermum parkii butter, 0.1000 to 4.0000 percent of sunflower seed oil and the balance of water. The cosmetic composition is prepared from the following components in percentage by weight: 1.0000 to 2.5000 percent of cetostearyl olivate, 0.5000 to 1.0000 percent of sorbitan olivate, 0.2000 to 0.4000 percent of stearyl alcohol, 0.1000 to 1.0000 percent of dioctyl dodecanol lauroyl glutamate, 1.0000 to 3.0000 percent of squalane, 0.5000 to 1.5000 percent of behenyl alcohol, 0.6000 to 1.0000 percent of white beeswax, 0.5000 to 1.0000 percent of phytosterol oleate, 0.0080 to 0.0100 percent of phytosterols, 0.3000 to 0.7000 percent of sunflower seed oil unsaponifiable matter, 0.5000 to 1.0000 percent of panthenol, 0.2000 to 0.7000 percent of dipropylene glycol, 0.1000 to 0.3000 percent of raspberry ketone, 0.0200 to The composition comprises the following components in percentage by weight: 0.0035 to 0.0065 percent of rosemary leaf extract, 0.0500 to 0.2000 percent of bisabolol, 0.0010 to 0.0030 percent of citric acid and the balance of deionized water. The lipid-like wrapping system provided by the invention has better air permeability and moisture retention.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and specifically, to a lipidoid encapsulation system, a preparation method thereof, and an application thereof. Background Art

[0002] Liposomes are ultramicro spherical vesicles formed by phospholipid bilayers. Phospholipid molecules have hydrophilic and lipophilic groups, which are regularly arranged in an aqueous phase to form a bilayer spherical structure. Water-soluble components can be encapsulated in the aqueous phase, and lipophilic components can be located between the bilayer lipid membranes. Therefore, liposomes can encapsulate both lipophilic molecules and water-soluble molecules at the same time. However, the structure of liposomes is mainly composed of phospholipids. During normal storage, phospholipid bonds are easily oxidized, generating phospholipid oxidation toxicity, and may also cause leakage of the encapsulated substances; at the same time, the price of phospholipids is high, which is not conducive to the encapsulation of low-cost substances.

[0003] The emergence of lipidoids overcomes these problems. Compared with liposomes, lipidoids are not only stable themselves, but also can increase the stability of the contents, and have diversity in structural characteristics (composition, fluidity, and size), and can be designed according to requirements. For example, it allows hydrophilic groups to contact the surface and can incorporate hydrophilic groups into the bilayer to change their in vivo behavior; lipidoids can also be further processed to delay the release of the contents, such as emulsifying the aqueous dispersion of lipidoids in a non-aqueous phase to regulate the release rate of the contents.

[0004] The skin thickness of infants and young children is only one-tenth of that of adult skin, and their stratum corneum has not yet developed maturely. This makes their skin cells have a higher water content but are prone to dehydration; they have a higher active permeability ability, but the skin barrier function is relatively weak. In view of this, developing a moisturizing product that is both safe and can effectively protect the delicate skin of infants has become an important problem that the industry urgently needs to solve. Summary of the Invention

[0005] Aiming at the defects in the prior art, the purpose of the present invention is to provide a lipidoid encapsulation system, a preparation method thereof, and an application thereof.

[0006] The technical solution of the present invention is as follows: The first aspect of the present invention provides a lipid - encapsulated system, and the lipid - encapsulated system comprises raw materials in the following mass fractions: 0.1000 - 0.5000 wt.% sodium hyaluronate, 2.0000 - 6.0000 wt.% glycerol, 0.5000 - 2.0000 wt.% butanediol, 0.1000 - 1.0000 wt.% acryloyldimethyltaurate / VP copolymer, 0.0100 - 0.1000 wt.% Tremella fuciformis Berk. fruit body extract, 0.0100 - 0.2000 wt.% ceramide NP, 1.0000 - 3.0000 wt.% coco - caprylate / caprate, 0.5000 - 3.0000 wt.% caprylic / capric triglyceride, 3.0000 - 5.0000 wt.% jojoba seed oil, 1.0000 - 5.0000 wt.% shea butter, 0.1000 - 4.0000 wt.% sunflower seed oil, 1.0000 - 2.5000 wt.% cetyl stearyl olivate, 0.5000 - 1.0000 wt.% sorbitan olivate, 0.2000 - 0.4000 wt.% stearyl alcohol, 0.1000 - 1.0000 wt.% dicaprylyl lauroyl glutamate, 1.0000 - 3.0000 wt.% squalane, 0.5000 - 1.5000 wt.% behenyl alcohol, 0.6000 - 1.0000 wt.% white beeswax, 0.5000 - 1.000 wt.% phytosteryl oleate, 0.0080 - 0.0100 wt.% phytosterols, 0.3000 - 0.7000 wt.% sunflower seed oil unsaponifiables, 0.5000 - 1.0000 wt.% panthenol, 0.2000 - 0.7000 wt.% dipropylene glycol, 0.1000 - 0.3000 wt.% raspberry ketone, 0.0200 - 0.2000 wt.% caprylyl glycol, 0.0200 - 0.0500 wt.% ethylhexylglycerin, 0.0035 - 0.0045 wt.% rosemary leaf extract, 0.0500 - 0.2000 wt.% bisabolol, 0.0010 - 0.0030 wt.% citric acid, and the balance is deionized water.

[0007] The second aspect of the present invention provides a preparation method of the above - mentioned lipid - encapsulated system, and the preparation method comprises the following steps: 1), Weigh the raw materials in the formula amount, and the raw materials include phase A raw materials, phase B raw materials, phase C raw materials, phase D raw materials, phase E raw materials, and phase F raw materials; The phase A raw materials include deionized water, sodium hyaluronate, glycerol, butanediol, acryloyldimethyltaurate / VP copolymer, and Tremella fuciformis Berk. fruit body extract; The phase B raw materials include: ceramide NP, coco - caprylate / caprate, and caprylic / capric triglyceride; The raw materials of Phase C include: jojoba seed oil, shea butter, sunflower seed oil, cetearyl olivate, sorbitan olivate, stearyl alcohol, dicaprylyl lauroyl glutamate, squalane, behenyl alcohol, white beeswax, phytosteryl oleate, phytosterols, and sunflower seed oil unsaponifiables; The raw materials of Phase D include: panthenol and deionized water; The raw materials of Phase E include: dipropylene glycol, raspberry ketone, caprylyl glycol, ethylhexylglycerin, sunflower seed oil, rosemary leaf extract, and bisabolol; The raw materials of Phase F include: citric acid and deionized water.

[0008] 2) Pretreatment: Place the raw materials of Phase B in a container, heat up to 80 - 90 °C, and stir until dissolved evenly to obtain the pre-dissolved Phase B; Place the raw materials of Phase D in another container, heat up to 40 - 50 °C, and stir until dissolved evenly to obtain the pre-dissolved Phase D; Place the raw materials of Phase F in another container, stir until dissolved evenly to obtain the pre-dissolved Phase F; 3) Put the components in Phase A into an emulsifying pot, heat up to 75 - 85 °C, and stir and disperse until evenly dispersed; 4) Put the components in Phase C into an oil phase pot, heat up to 75 - 85 °C, then add the pre-dissolved Phase B, and stir and disperse until evenly dispersed; 5) Keep the temperature at 75 - 85 °C, filter the materials in the oil phase pot into the emulsifying pot, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 2800 - 3000 revolutions per minute until emulsified evenly; 6) Lower the temperature of the emulsifying pot to 40 - 45 °C, add the pre-dissolved Phase D, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 7) Keep the temperature of the emulsifying pot at 40 - 45 °C, add the raw materials of Phase F, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 8) Lower the temperature to below 38 °C, stop stirring, inspect, filter, and discharge to obtain the lipid - based encapsulation system.

[0009] Further, in step 3), stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 2000 - 3000 revolutions per minute for 5 - 20 minutes; In step 4), stir at 20 - 40 revolutions per minute for 10 - 30 minutes.

[0010] Further, in the raw materials of Phase A, the mass ratio of deionized water, sodium hyaluronate, glycerol, butanediol, acryloyldimethyltaurate / VP copolymer, and Tremella fuciformis Berk. fruit body extract is 70.0445:0.1010:4.0000:1.0000:0.5000:0.0200.

[0011] Further, in the raw materials of Phase B, the mass ratio of ceramide NP, coco-caprylate / caprate, and caprylic / capric triglyceride is 0.1010:2.0000:1.5000.

[0012] Further, in the raw materials of Phase C, the mass ratio of stearyl alcohol, dicaprylyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, shea butter, jojoba seed oil, phytosteryl oleate, phytosterols, sunflower seed oil, white beeswax, squalane, behenyl alcohol, and sunflower seed oil unsaponifiables is 0.3000:0.7000:1.2000:0.8000:3.0000:4.0000:0.5910:0.0090:3.0000:0.8000:2.0000:1.0000:0.5000.

[0013] Further, in the raw materials of Phase D, the mass ratio of panthenol and deionized water is 0.8000:0.8000.

[0014] Further, in the raw materials of Phase E, the mass ratio of dipropylene glycol, raspberry ketone, caprylyl glycol, ethylhexylglycerin, sunflower seed oil, rosemary leaf extract, and bisabolol is 0.6500:0.2000:0.1200:0.0300:0.1045:0.0055:0.1010.

[0015] Further, in the raw materials of Phase F, the mass ratio of citric acid and deionized water is 0.0025:0.0200.

[0016] The third aspect of the present invention provides the application of the lipid encapsulation system in the preparation of infant and toddler moisturizing skin cream products, and the lipid encapsulation system is the lipid encapsulation system described in the first aspect of the present invention or the lipid encapsulation system prepared by the preparation method described in the second aspect of the present invention.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. The lipidoid encapsulation system provided by the present invention changes the traditional spherical liquid crystal liposome mainly composed of phospholipids. This system combines lamellar liquid crystal with cholesterol to form a new type of lipidosome. This combination method not only significantly improves the stability of the system, effectively solves the toxicity problem caused by phospholipid oxidation, but also the lamellar liquid crystal structure is similar to the skin sebum structure, and the two have good compatibility. When combined with more hydrophobic cholesterol, it forms a skin protection layer, which is beneficial to strengthening the skin barrier and plays a good protective role.

[0018] 2. The preparation method of the lipidoid encapsulation system provided by the present invention innovatively uses the dual liquid crystal technology, that is, a liquid crystal emulsion system is used to encapsulate and construct nutrients for children's skin elements. The liquid crystal emulsion system consists of two liquid crystal sources: one is the biomimetic liquid crystal of olive oil source liquid, its structure is a crystal structure, similar to the structure of human skin sebum, with strong skin affinity, and there is a large amount of bound water between the lamellae. These waters are not easy to volatilize, forming a water storage layer on the skin surface, which can keep the skin moist for a long time; the other is the liquid crystal of ceramide-like. The human skin is formed by stratum corneum cells and intercellular lipids. Ceramide occupies most of the intercellular lipids in the stratum corneum cells and forms a lamellar liquid crystal structure with cholesterol and fatty acids, etc. It is an important active ingredient constituting the skin barrier function. The liquid crystal system constructed with ceramide-like can strengthen and guarantee the barrier repair effect of the formula.

[0019] 3. The embedding of the two liquid crystals in the lipidoid encapsulation system provided by the present invention is complementary, enhancing the stability and uniformity of the liquid crystal morphology. At the same time, the special structure of the lamellar liquid crystal enables the active ingredients in the encapsulated content to be slowly released, ensuring that the effect of the product can last longer. Description of the Drawings

[0020] By reading the detailed description of the non-restrictive embodiments with reference to the following drawings, other features, purposes and advantages of the present invention will become more obvious: Figure 1 It is a schematic diagram of the polarized light microscope observation result of the lipidoid encapsulation system provided by the experimental example of the present invention; Figure 2 It is a schematic diagram of the release behavior standard curve results of Vc ethyl ether and Ve acetate in different release media provided by the experimental example of the present invention ( Figure 2 in which a is: the standard curve of Vc ethyl ether in pH 7.4 phosphate buffer solution; Figure 2 in which b is: the standard curve of Ve acetate in ethanol); Figure 3 It is a schematic diagram of the release behavior results of Vc ethyl ether and Ve acetate in the lipidoid encapsulation system provided by the experimental example of the present invention; Figure 4 It is a schematic diagram of the water sealing test result of the lipidoid encapsulation system provided by the experimental example of the present invention; Figure 5 Schematic diagram of the RNA sequencing heat map results of three complexes provided by the experimental examples of the present invention; Figure 6 Schematic diagram of the GO enrichment analysis results of three complexes provided by the experimental examples of the present invention ( Figure 6 In a, it is a schematic diagram of the results of the down-regulation of genes in the GO enrichment analysis of lipid inclusion bodies; Figure 6 In b, it is a schematic diagram of the results of the up-regulation of genes in the GO enrichment analysis of lipid inclusion bodies; Figure 6 In c, it is a schematic diagram of the results of the down-regulation of genes in the GO enrichment analysis of the double liquid crystal emulsion system; Figure 6 In d, it is a schematic diagram of the results of the up-regulation of genes in the GO enrichment analysis of the double liquid crystal emulsion system; Figure 6 In e, it is a schematic diagram of the results of the GO enrichment analysis of multi-moisturizing molecules); Figure 7 Schematic diagram of the KEGG pathway analysis results of three complexes provided by the experimental examples of the present invention; Figure 8 Schematic diagram of the RTCA experiment results of three complexes provided by the experimental examples of the present invention; Figure 9 Schematic diagram of the experimental results of the gene expression levels of three complexes provided by the experimental examples of the present invention. Detailed implementation manners

[0021] The present invention will be described in detail below with reference to specific embodiments. The following embodiments will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several changes and improvements can still be made, and these all belong to the protection scope of the present invention.

[0022] First embodiment This embodiment provides a lipid inclusion system and its preparation method. The lipid inclusion system in this embodiment includes the following raw materials in mass fractions: 70.8645 wt.% deionized water, 0.1010 wt.% sodium hyaluronate, 4.0000 wt.% glycerin, 1.0000 wt.% butanediol, 0.5000 wt.% acryloyldimethyltaurate / VP copolymer, 0.0200 wt.% Tremella fuciformis Berk. fruit body extract, 0.1010 wt.% ceramide NP, 2.0000 wt.% coco-caprylate / caprate, 1.5000 wt.% caprylic / capric triglyceride, 4.0000 wt.% jojoba seed oil, 3.0000 wt.% shea butter, 3.1045 wt.% sunflower seed oil, 1.2000 wt.% cetearyl olivate, 0.8000 wt.% sorbitan olivate, 0.3000 wt.% stearyl alcohol, 0.7000 wt.% dicaprylyl lauroyl glutamate, 2.0000 wt.% squalane, 1.0000 wt.% behenyl alcohol, 0.8000 wt.% white beeswax, 0.5910 wt.% phytosteryl oleate, 0.0090 wt.% phytosterols, 0.5000 wt.% sunflower seed oil unsaponifiables, 0.8000 wt.% panthenol, 0.6500 wt.% dipropylene glycol, 0.2000 wt.% raspberry ketone, 0.1200 wt.% caprylyl glycol, 0.0300 wt.% ethylhexylglycerin, 0.0055 wt.% Rosmarinus officinalis leaf extract, 0.1010 wt.% bisabolol, 0.0025 wt.% citric acid.

[0023] The preparation method of the lipid-coated system in this example includes the following steps: 1), Weigh the raw materials in the formula amount, classify the raw materials into A-phase raw materials, B-phase raw materials, C-phase raw materials, D-phase raw materials, E-phase raw materials, and F-phase raw materials; The A-phase raw materials include deionized water, sodium hyaluronate, glycerin, butanediol, acryloyldimethyltaurate / VP copolymer, and Tremella fuciformis Berk. fruit body extract, and the mass ratio is 70.0445:4.0000:1.0000:0.0700:0.0310; The B-phase raw materials include: ceramide NP, coco-caprylate / caprate, caprylic / capric triglyceride, and the mass ratio is 0.1010:2.0000:1.5000; The raw materials of Phase C include: stearyl alcohol, dicaprylyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, shea butter, jojoba seed oil, phytosteryl oleate, phytosterols, sunflower seed oil, white beeswax, squalane, behenyl alcohol, unsaponifiables of sunflower seed oil, and the mass ratio is 0.3000:0.7000:1.2000:0.8000:3.0000:4.0000:0.5910:0.0090:3.0000:0.8000:2.0000:1.0000:0.5000; The raw materials of Phase D include: panthenol, deionized water, and the mass ratio is 0.8000:0.8000; The raw materials of Phase E include: dipropylene glycol, raspberry ketone, caprylyl glycol, ethylhexylglycerin, sunflower seed oil, rosemary leaf extract, bisabolol, and the mass ratio is 0.6500:0.2000:0.1200:0.0300:0.1045:0.0055:0.1010; The raw materials of Phase F include: citric acid, deionized water, and the mass ratio is 0.0025:0.0200.

[0024] 2) Pretreatment: Place the raw materials of Phase B in a container, heat up to 80 - 90 °C, and stir until dissolved evenly to obtain the pre-dissolved Phase B; Place the raw materials of Phase D in another container, heat up to 40 - 50 °C, and stir until dissolved evenly to obtain the pre-dissolved Phase D; Place the raw materials of Phase F in another container, and stir until dissolved evenly to obtain the pre-dissolved Phase F; 3) Put the components in Phase A into an emulsifying pot, heat up to 75 - 85 °C, and stir and disperse until evenly dispersed; 4) Put the components in Phase C into an oil phase pot, heat up to 75 - 85 °C, then add the pre-dissolved Phase B, and stir and disperse until evenly dispersed; 5) Keep the temperature at 75 - 85 °C, filter the materials in the oil phase pot into the emulsifying pot, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 2800 - 3000 revolutions per minute until emulsified evenly; 6) Lower the temperature of the emulsifying pot to 40 - 45 °C, add the pre-dissolved Phase D, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 7) Keep the temperature of the emulsifying pot at 40 - 45 °C, add the raw materials of Phase F, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 8) Lower the temperature to below 38 °C, stop stirring, inspect, filter, and discharge to obtain the lipid - encapsulated system.

[0025] In step 3), stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 2000 - 3000 revolutions per minute for 5 - 20 minutes; In step 4), stir at 20 - 40 revolutions per minute for 10 - 30 minutes.

[0026] Second Embodiment This embodiment provides a lipid - based encapsulation system and its preparation method. The lipid - based encapsulation system in this embodiment comprises raw materials with the following mass fractions: 0.1000 wt.% sodium hyaluronate, 2.0000 wt.% glycerol, 0.5000 wt.% butylene glycol, 0.1000 wt.% acryloyldimethyltaurate / VP copolymer, 0.0100 wt.% Tremella fuciformis Berk. fruit body extract, 0.0100 wt.% ceramide NP, 1.0000 wt.% coco - caprylate / caprate, 0.5000 wt.% caprylic / capric triglyceride, 3.0000 wt.% jojoba seed oil, 1.0000 wt.% shea butter, 0.2000 wt.% sunflower seed oil, 1.0000 wt.% cetearyl olivate, 0.5000 wt.% sorbitan olivate, 0.2000 wt.% stearyl alcohol, 0.1000 wt.% dicaprylyl lauroyl glutamate, 1.0000 wt.% squalane, 0.5000 wt.% behenyl alcohol, 0.6000 wt.% white beeswax, 0.5000 wt.% phytosteryl oleate, 0.0080 wt.% phytosterols, 0.3000 wt.% sunflower seed oil unsaponifiables, 0.5000 wt.% panthenol, 0.2000 wt.% dipropylene glycol, 0.1000 wt.% raspberry ketone, 0.0200 wt.% caprylyl glycol, 0.0200 wt.% ethylhexylglycerin, 0.0035 wt.% rosemary leaf extract, 0.0500 wt.% bisabolol, 0.0010 wt.% citric acid, and the balance is deionized water.

[0027] The preparation method of the lipid - based encapsulation system in this embodiment comprises the following steps: 1), Weigh the raw materials in the formulated amounts, classify the raw materials into phase A raw materials, phase B raw materials, phase C raw materials, phase D raw materials, phase E raw materials, and phase F raw materials; Phase A raw materials include deionized water, sodium hyaluronate, glycerol, butylene glycol, acryloyldimethyltaurate / VP copolymer, and Tremella fuciformis Berk. fruit body extract, and the mass ratio is 87.3595:0.1000:2.0000:0.5000:0.0100:0.0100; Phase B raw materials include: ceramide NP, coco - caprylate / caprate, caprylic / capric triglyceride, and the mass ratio is 0.0100:1.0000:0.5000; The raw materials of Phase C include: stearyl alcohol, dicaprylyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, shea butter, jojoba seed oil, phytosteryl oleate, phytosterols, sunflower seed oil, white beeswax, squalane, behenyl alcohol, and sunflower seed oil unsaponifiables, with a mass ratio of 0.2000:0.1000:1.0000:0.5000:1.0000:3.0000:0.5000:0.0080:0.1000:0.6000; The raw materials of Phase D include: panthenol and deionized water, with a mass ratio of 0.5000:0.5000; The raw materials of Phase E include: dipropylene glycol, raspberry ketone, caprylyl glycol, ethylhexylglycerin, sunflower seed oil, rosemary leaf extract, and bisabolol, with a mass ratio of 0.2000:0.1000:0.0200:0.0200:0.1000:0.0035:0.0500; The raw materials of Phase F include: citric acid and deionized water, with a mass ratio of 0.0010:0.0080.

[0028] 2) Pretreatment: Place the raw materials of Phase B in a container, heat up to 80 - 90 °C, and stir until dissolved and homogeneous to obtain the pre-dissolved Phase B; Place the raw materials of Phase D in another container, heat up to 40 - 50 °C, and stir until dissolved and homogeneous to obtain the pre-dissolved Phase D; Place the raw materials of Phase F in another container, and stir until dissolved and homogeneous to obtain the pre-dissolved Phase F; 3) Put the components in Phase A into an emulsifying pot, heat up to 75 - 85 °C, and stir and disperse until evenly dispersed; 4) Put the components in Phase C into an oil phase pot, heat up to 75 - 85 °C, then add the pre-dissolved Phase B, and stir and disperse until evenly dispersed; 5) Keep the temperature at 75 - 85 °C, filter the materials in the oil phase pot into the emulsifying pot, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 2800 - 3000 revolutions per minute until emulsified evenly; 6) Cool down the temperature in the emulsifying pot to 40 - 45 °C, add the pre-dissolved Phase D, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 7) Keep the temperature in the emulsifying pot at 40 - 45 °C, add the raw materials of Phase F, stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 8) Cool down to below 38 °C, stop stirring, inspect, filter, and discharge to obtain the lipid - encapsulated system.

[0029] In step 3), stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 2000 - 3000 revolutions per minute for 5 - 20 minutes; In step 4), stir at 20 - 40 revolutions per minute for 10 - 30 minutes.

[0030] Third Embodiment This embodiment provides a lipid - encapsulated system and its preparation method. The lipid - encapsulated system in this embodiment comprises raw materials with the following mass fractions: 0.5000 wt.% sodium hyaluronate, 6.0000 wt.% glycerol, 2.0000 wt.% butylene glycol, 1.0000 wt.% acryloyldimethyltaurate / VP copolymer, 0.1000 wt.% Tremella fuciformis Berk. fruit body extract, 0.2000 wt.% ceramide NP, 3.0000 wt.% coco - caprylate / caprate, 3.0000 wt.% caprylic / capric triglyceride, 5.0000 wt.% jojoba seed oil, 5.0000 wt.% shea butter, 4.0000 wt.% sunflower seed oil, 2.5000 wt.% cetearyl olivate, 1.0000 wt.% sorbitan olivate, 0.4000 wt.% stearyl alcohol, 1.0000 wt.% dicaprylyl lauroyl glutamate, 3.0000 wt.% squalane, 1.5000 wt.% behenyl alcohol, 1.0000 wt.% white beeswax, 1.0000 wt.% phytosteryl oleate, 0.0100 wt.% phytosterols, 0.7000 wt.% sunflower seed oil unsaponifiables, 1.0000 wt.% panthenol, 0.7000 wt.% dipropylene glycol, 0.3000 wt.% raspberry ketone, 0.2000 wt.% caprylyl glycol, 0.0500 wt.% ethylhexylglycerin, 0.0065 wt.% rosemary leaf extract, 0.2000 wt.% bisabolol, 0.0030 wt.% citric acid, and the balance is deionized water.

[0031] The preparation method of the lipid - encapsulated system in this embodiment comprises the following steps: 1), Weigh the raw materials in the formula amounts, classify the raw materials into A - phase raw materials, B - phase raw materials, C - phase raw materials, D - phase raw materials, E - phase raw materials, and F - phase raw materials; The A - phase raw materials include deionized water, sodium hyaluronate, glycerol, butylene glycol, acryloyldimethyltaurate / VP copolymer, and Tremella fuciformis Berk. fruit body extract, and the mass ratio is 54.8065:0.5000:6.0000:2.0000:1.0000:0.1000; The B - phase raw materials include: ceramide NP, coco - caprylate / caprate, and caprylic / capric triglyceride, and the mass ratio is 0.2000:3.0000:3.0000; The raw materials of Phase C include: stearyl alcohol, dicaprylyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, shea butter, jojoba seed oil, phytosterol oleate, phytosterols, sunflower seed oil, white beeswax, squalane, behenyl alcohol, unsaponifiables of sunflower seed oil, and the mass ratio is 0.4000:1.0000:2.5000:0.8000:5.0000:5.0000:1.0000:0.0100:3.5000:1.0000:3.0000:1.5000:0.7000; The raw materials of Phase D include: panthenol, deionized water, and the mass ratio is 1.0000:1.0000; The raw materials of Phase E include: dipropylene glycol, raspberry ketone, caprylyl glycol, ethylhexylglycerin, sunflower seed oil, rosemary leaf extract, bisabolol, and the mass ratio is 0.7000:0.3000:0.2000:0.0500:0.5000:0.0065:0.2000; The raw materials of Phase F include: citric acid, deionized water, and the mass ratio is 0.0030:0.0240.

[0032] 2), Pretreatment: Place the raw materials of Phase B in a container, heat up to 80 - 90 °C, and stir until dissolved evenly to obtain the pre-dissolved Phase B; Place the raw materials of Phase D in another container, heat up to 40 - 50 °C, and stir until dissolved evenly to obtain the pre-dissolved Phase D; Place the raw materials of Phase F in another container, and stir until dissolved evenly to obtain the pre-dissolved Phase F; 3), Put each component in Phase A into an emulsifying pan, heat up to 75 - 85 °C, and stir and disperse until evenly dispersed; 4), Put each component in Phase C into an oil phase pan, heat up to 75 - 85 °C, then add the pre-dissolved Phase B, and stir and disperse until evenly dispersed; 5), Keep the temperature at 75 - 85 °C, filter the materials in the oil phase pan into the emulsifying pan, stir at 20 - 40 revolutions per minute, stir for 10 - 30 minutes, and homogenize at 2800 - 3000 revolutions per minute until emulsified evenly; 6), Cool down the temperature of the emulsifying pan to 40 - 45 °C, add the pre-dissolved Phase D, stir at 20 - 40 revolutions per minute, stir for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 7), Keep the temperature of the emulsifying pan at 40 - 45 °C, add the raw materials of Phase F, stir at 20 - 40 revolutions per minute, stir for 10 - 30 minutes, and homogenize at 1000 - 1200 revolutions per minute until evenly dispersed; 8), Cool down to below 38 °C, stop stirring, inspect, filter, and discharge to obtain the lipid - encapsulated system.

[0033] In step 3), stir at 20 - 40 revolutions per minute for 10 - 30 minutes, and homogenize at 2000 - 3000 revolutions per minute for 5 - 20 minutes; In step 4), stir at 20 - 40 revolutions per minute for 10 - 30 minutes.

[0034] Application Example This application example provides the application of the lipid - based encapsulation system in the preparation of infant moisturizing skin cream products. In this application example, the lipid - based encapsulation system and its preparation method refer to the above - mentioned embodiments.

[0035] Experimental Example Through the following experiments in this experimental example, it is proved that the lipid - based encapsulation system provided by the first embodiment can achieve the sustained release of the content through the encapsulation of lamellar liquid crystals, ensuring the safer and more durable use of the product.

[0036] 1.1 Observation with a Polarizing Microscope Take a small amount of the lipid - based encapsulation system and place it on a glass slide. Press it with a coverslip until it becomes semi - transparent, and then observe the microscopic structure of the sample under a polarizing microscope (Leica DM4 P upright polarizing microscope). The observation results are as Figure 1 shown. The lipid - based encapsulation system shows an obvious Maltese cross structure under the polarizing microscope, indicating that the lipid - based encapsulation system in this experimental example has a lamellar liquid crystal structure.

[0037] 1.2 Sustained Release Performance Test The sustained release of the lipid - based encapsulation system was tested by the dynamic dialysis method. The operation process is as follows: Weigh 2 g of each of the lipid - based encapsulation systems containing 2 wt.% 3 - O - ethyl ascorbic acid (Vc ethyl ether) and 2 wt.% tocopheryl acetate (Ve acetate) respectively and place them in dialysis bags (RC, MD34 - 3500D). Tie the dialysis bags and place them in 200 mL of release medium. Stir magnetically at 25 °C with a rotation speed of 50 - 100 rpm. Sampling is carried out at different time points, 5 mL each time, and at the same time, 5 mL of release medium is replenished. After filtering the sampled solution through a 0.45 μm needle - type filter, use an ultraviolet spectrophotometer to measure the concentration of Vc ethyl ether or Ve acetate in the release medium, and calculate its cumulative release rate. Select pH7.4 phosphate buffer as the release medium for Vc ethyl ether and ethanol as the release medium for Ve acetate. The standard curves of Vc ethyl ether and Ve acetate were measured by an ultraviolet spectrophotometer (Algilent Cary 60 UV - Vis, 280 nm) respectively, as Figure 2 shown.

[0038] Layered liquid crystals are mainly composed of amphiphilic surfactant bilayers and interlayer bound water, so they can encapsulate both hydrophilic and lipophilic active substances. Among them, water-soluble active substances are mainly encapsulated in the water channel region between the amphiphilic surfactant layers, while lipophilic active substances are mainly concentrated in the hydrophobic region of the surfactant bilayers and the encapsulated oil phase. Therefore, the sustained-release behavior of the lipid-based encapsulation system was characterized by water-soluble Vc ethyl ether and lipophilic Ve acetate respectively.

[0039] Using pH 7.4 phosphate buffer as the release medium, the cumulative sustained-release rate of Vc ethyl ether was studied, and using ethanol as the release medium, the cumulative release rate of Ve acetate was studied. The results are as Figure 3 shown (the cumulative release standard curves of Vc ethyl ether and Ve acetate were fitted by the first-order kinetic equation, and the regression coefficients (R2) of the fitting equations were 0.985 and 0.995 respectively, indicating that the release of Vc ethyl ether and Ve acetate is a diffusion-dominated process). Compared with Vc ethyl ether, the lipid-based encapsulation system has a more obvious sustained-release effect on lipophilic Ve acetate. From 0 to 100 min, the release of Ve acetate is relatively fast, and the cumulative release rate reaches 49% at 90 min. From 100 to 420 min, the release rate of the active substance begins to slow down, and the maximum value of about 75% can be reached at about 420 min. Compared with Ve acetate, the release rate of Vc ethyl ether is faster, reaching 50% cumulative release at 30 min and the maximum release value of 100% at 120 min. It is speculated that part of the water-soluble Vc ethyl ether exists in the external water phase, and the other part is encapsulated in the bound water between the amphiphilic surfactant layers. This shows that the lipid-based encapsulation system in this experimental example has a good sustained-release effect on the release of the lipid components contained therein.

[0040] 1.3 Sealing test Imitating the grease sealing experiment, 20.0000 g of deionized water was placed in a 50 mL centrifuge tube. After sealing with filter paper, 0.2000 g of petrolatum and 0.2000 g of the lipid-based encapsulation system (Cream) were evenly applied to the sealing part respectively. After being placed in an oven at 50 °C for 17 hours, the weight change of water before and after was measured. The results are as Figure 4 shown. Compared with the Petrolatum group with strong sealing property, the Cream group in this experimental example allows water vapor to pass through, but compared with the Blank group (blank sample, without any treatment on the seal), the Cream group can reduce water evaporation. This shows that the lipid-based encapsulation system in this experimental example not only has a breathable effect during use, but also can reduce the loss of skin moisture, having a certain degree of moisturizing effect. This characteristic enables the lipid-based encapsulation system to form a protective lipophilic film on the skin and strengthen the skin barrier.

[0041] 1.4 RNA Sequencing and Analysis RNA in cells was extracted using the TRIzol method. Library construction and RNA sequencing were performed by Shanghai OE Biotech Co., Ltd. The DESeq2 software was used to analyze the gene expression differences in the lipid-coated system. False discovery rate (FDR) < 0.05 and absolute fold change ≥ 2 were used as parameters to identify differentially expressed genes. Molecular function, cellular component, and biological process are the three components of Gene Ontology (GO), a globally standardized gene classification system. GO terms with p-value < 0.05 were defined as significantly enriched GO terms among DEGs (differentially expressed genes). According to the pathway enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG), compared with the whole genome background, DEGs were significantly enriched in metabolic or signal transduction pathways. A p-value < 0.05 was considered statistically significant. Gene Set Enrichment Analysis (GSEA) was performed using GSEA (v4.1.0) and MSigDB to determine whether a set of genes showed significant differences in certain GO keywords or KEGG pathways. GO terms and KEGG pathways meeting the following requirements were considered different between the two groups: |NES| > 1, NOM p-value < 0.05, FDR q-value < 0.25.

[0042] In this experiment, three complexes in the lipid-coated system were extracted and named multi-moisturizing molecule, dual liquid crystal emulsion system, and lipid inclusion, respectively.

[0043] The multi-moisturizing molecule includes: sodium hyaluronate, tremella fruiting body extract, glycerin, butylene glycol, and panthenol.

[0044] The dual liquid crystal emulsion system includes: stearyl alcohol, dicaprylyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, phytosteryl oleate, phytosterols, squalane, and jojoba oil.

[0045] The lipid inclusion includes: sunflower seed oil, jojoba oil, shea butter, white beeswax, caprylic / capric triglyceride, ceramide NP, squalane, phytosteryl oleate, and glycerin.

[0046] 1.4.1 Differential Gene Analysis The heatmap results of RNA sequencing are as Figure 5As shown, significant expression changes occurred in multiple genes related to skin barrier function in the three complexes (multi-moisturizing molecules, lipid vesicles, and double liquid crystal emulsion systems). Among the upregulated genes, the expressions of FGF19 and SMAD5 increased, and these genes are related to skin repair and enhanced barrier function. In addition, the upregulation of CD244 and CRLF1 implies enhanced immune regulation function. A series of genes related to inflammatory responses and cellular stress were downregulated. The downregulation of SERPINA3 and RASGRP1 may indicate the inhibition of the inflammatory pathway, reduction of skin inflammation levels, and thus improvement of barrier stability. In addition, the downregulation of the expressions of STPG2 and ZSCAN5B may be related to the inhibition of cellular stress responses. The differential expressions of these genes indicate that the lipid vesicle system provided by the present invention improves the skin barrier function by promoting skin barrier repair, enhancing cellular functions, and alleviating inflammatory responses.

[0047] 1.4.2 GO Enrichment Analysis The results of the GO enrichment analysis are as Figure 6 shown.

[0048] The genes significantly enriched in the lipid inclusions and bicontinuous cubic emulsion system are mainly related to the pathways associated with extracellular matrix and cell adhesion. These pathways include extracellular region, extracellular space, cell adhesion, collagen-containing extracellular matrix, and basement membrane. These changes indicate that the lipid inclusion system provided by the present invention helps to improve the structural stability and repair ability of the skin barrier by promoting the remodeling of the extracellular matrix and enhancing cell-cell adhesion. On the other hand, the genes significantly downregulated are mainly enriched in the pathways related to inflammation and immune response, including inflammatory response to wounding, Toll-like receptor signaling pathway, peptidoglycan immune receptor activity, and cellular response to transforming growth factor beta stimulus. The downregulation of these pathways indicates that the lipid inclusion system provided by the invention helps to maintain the balanced state of the barrier by reducing inflammation and immune responses and alleviating the inflammatory burden of the skin. The genes of the multi-moisturizing molecules are significantly enriched in multiple pathways related to cell function and signal regulation. These pathways include Peyer’s patch development, protein-arginine deiminase activity, intracellular cAMP-activated cation channel activity, and positive regulation of hepatic stellate cell activation. These changes indicate that the lipid inclusion system provided by the present invention affects signal transduction, cell activation, and immune regulation processes.

[0049] 1.4.3 KEGG Enrichment Analysis The results of KEGG pathway analysis are as Figure 7As shown, the differentially expressed genes of the three complexes were significantly enriched in multiple key signaling pathways and metabolic pathways. These pathways include the cAMP signaling pathway, the MAPK signaling pathway, and glycerophospholipid metabolism, indicating that the lipid-based encapsulation system provided by the present invention can promote the proliferation and differentiation of keratinocytes, enhance the function of tight junctions, further accelerate the repair and stabilization of the barrier, and regulate the generation of important components of the skin barrier lipid layer, improving the water-locking ability and structural integrity of the barrier. The genes were also significantly enriched in cytokine-cytokine receptor interaction and T cell receptor signaling pathway, indicating that the lipid-based encapsulation system provided by the present invention optimizes the immune defense ability of the barrier by regulating pro-inflammatory and anti-inflammatory signals and enhancing the skin immune surveillance function.

[0050] 1.5 RTCA experiment In this experiment, three complexes were extracted from the lipid-based encapsulation system and named multi-moisturizing molecules, dual liquid crystal emulsion system, and lipid-based inclusion body respectively.

[0051] The multi-moisturizing molecules include: sodium hyaluronate, Tremella fuciformis fruiting body extract, glycerol, butanediol, and panthenol.

[0052] The dual liquid crystal emulsion system includes: stearyl alcohol, diisooctyl dodecyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, phytosteryl oleate, phytosterols, squalane, and jojoba oil.

[0053] The lipid-based inclusion body includes: sunflower seed oil, jojoba oil, shea butter, white beeswax, caprylic / capric triglyceride, ceramide NP, squalane, phytosteryl oleate, and glycerol.

[0054] Human immortalized keratinocytes HaCaT were cultured in DMEM medium supplemented with 10% FBS and 1% penicillin-streptomycin solution (100 µg / mL) and passaged after 24 hours of culture in a cell incubator (37 °C, 5% CO 2 ) and inoculated with logarithmic-phase HaCaT cells on the E-Plate detection plate of the Real-Time Cell Analyzer (RTCA) SP instrument (1×10 4Cells / well), wait for 12 hours. After the cells adhered to the wall, aspirate and discard the culture medium, and add culture media containing the samples to be tested at different concentrations (the concentrations of the three complexes are 0.0150 wt.%, 0.03125 wt.%, 0.0625 wt.%, 0.1250 wt.%, 0.2500 wt.%, 0.5000 wt.%, 1.0000 wt.% respectively), and monitor the cell index every 10 minutes.

[0055] NC refers to the control group, and no treatment is performed on HaCaT cells.

[0056] The RTCA cell proliferation curve shows the effect of the lipid-coated system on HaCaT cells. The results are as Figure 8 shown. There is no significant difference in cell proliferation between the three complexes and the control group. This result indicates that the lipid-coated system provided by the present invention has no cytotoxicity to HaCaT cells, and the lipid-coated system provided by the present invention has good safety.

[0057] 1.6 Gene expression level test In this experiment, three complexes in the lipid-coated system were extracted and named multi-moisturizing molecules, dual liquid crystal emulsion system, and lipid-coated inclusion respectively.

[0058] The multi-moisturizing molecules include: sodium hyaluronate, Tremella fuciformis Berk. fruit body extract, glycerol, butylene glycol, and panthenol.

[0059] The dual liquid crystal emulsion system includes: stearyl alcohol, diisooctyl dodecyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, phytosterol oleate, phytosterols, squalane, and jojoba oil.

[0060] The lipid-coated inclusion includes: sunflower seed oil, jojoba oil, shea butter, white beeswax, caprylic / capric triglyceride, ceramide NP, squalane, phytosterol oleate, and glycerol.

[0061] HaCaT cells were seeded in 6-well plates (3×10 5Cells / well), after culturing for 24 hours, aspirate and discard the culture medium, wash the cells with PBS solution, then aspirate and discard the PBS, and add culture media containing the test samples at different concentrations (the concentrations of the multi-moisture molecules are 0.0150 wt.%, 0.0310 wt.%, and 0.06250 wt.% respectively, and the concentrations of the lipid vesicles and the bicontinuous cubic liquid crystal emulsion system are 0.0150 wt.%, 0.1250 wt.%, and 1.0000 wt.% respectively). After culturing for 24 hours, collect the cells and extract the total cellular RNA by the TRIzol method, and detect the RNA purity and concentration using NanoDrop. Synthesize cDNA using a reverse transcription kit, use the relevant primers and qPCR premix, and perform gene phenotype detection on a fluorescence quantitative PCR instrument. The details of the gene primers are shown in Table 1.

[0062] The qPCR results are as Figure 9 shown, NC refers to the control group, where no treatment is performed on HaCaT cells. Compared with the control group, KRT5, E-cadherin, and Occludin in the three complexes are all increased to a certain extent. Among them, the upregulation of KRT5 indicates enhanced activity of basal keratinocytes, which helps to promote skin regeneration. The increased expression of E-cadherin suggests improved cell-cell adhesion ability, which helps to maintain the integrity of the epidermal structure. The increase in Occludin indicates enhanced tight junctions, which helps to improve the skin barrier function. The above results indicate that the lipid vesicle system provided by the present invention can potentially improve the skin barrier function to a certain extent by enhancing epidermal cell connections and promoting basal cell activity.

[0063] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the above specific embodiments, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention. Without conflict, the embodiments of the present application and the features in the embodiments can be combined arbitrarily.

Claims

1. A lipid encapsulation system, characterized in that: The lipid encapsulation system comprises the following raw materials in mass fractions: 0.1000-0.5000wt.% sodium hyaluronate, 2.0000-6.0000wt.% glycerol, 0.5000-2.0000wt.% butanediol, 0.1000-1.0000wt.% ammonium acryloyldimethyltaurate / VP copolymer, 0.0100-0.1000wt.% Tremella fruiting body extract, 0.0100-0.2000wt.% ceramide NP, 1.0000-3.000wt.% 0wt.% Coconut oil-caprylate / caprate, 0.5000-3.0000wt.% Caprylic / capric triglyceride, 3.0000-5.0000wt.% Jojoba seed oil, 1.0000-5.0000wt.% Shea butter, 0.1000-4.0000wt.% Sunflower seed oil, 1.0000-2.5000wt.% Cetearyl olive oil ester, 0.5000-1.0000wt.% Sorbitan olive oil ester, 0.2000-0.4000wt.% wt.% stearyl alcohol, 0.1000-1.0000wt.% dioctyldodecyl lauroyl glutamate, 1.0000-3.0000wt.% squalane, 0.5000-1.5000wt.% behenyl alcohol, 0.6000-1.0000wt.% white beeswax, 0.5000-1.0000wt.% phytosterol oleate, 0.0080-0.0100wt.% phytosterols, 0.3000-0.7000wt.% sunflower seed oil unsaponifiable matter, 0.50 00-1.0000wt.% panthenol, 0.2000-0.7000wt.% dipropylene glycol, 0.1000-0.3000wt.% raspberry ketone, 0.0200-0.2000wt.% caprylyl glycol, 0.0200-0.0500wt.% ethylhexylglycerin, 0.0035-0.0065wt.% rosemary leaf extract, 0.0500-0.2000wt.% bisabolol, 0.0010-0.0030wt.% citric acid, and the balance is deionized water.

2. The method for preparing the lipid encapsulation system according to claim 1, characterized in that: The preparation method comprises the following steps: 1) Weigh the raw materials in the formula, which are divided into phase A raw materials, phase B raw materials, phase C raw materials, phase D raw materials, phase E raw materials, and phase F raw materials; The phase A raw materials include deionized water, sodium hyaluronate, glycerin, butylene glycol, ammonium acryloyldimethyltaurate / VP copolymer, and Tremella fuciformis fruiting body extract; The phase B raw materials include: ceramide NP, coconut oil alcohol-caprylate / caprate, caprylic / capric triglyceride; The phase C raw materials include: jojoba seed oil, avocado tree butter, sunflower seed oil, cetearyl olivate, sorbitan olivate, stearyl alcohol, dioctyldodecyl lauroyl glutamate, squalane, behenyl alcohol, white beeswax, phytosteryl oleate, phytosterols, and unsaponifiable matter of sunflower seed oil; The phase D raw materials include: panthenol and deionized water; The raw materials of phase E include: dipropylene glycol, raspberry ketone, caprylyl glycol, ethylhexylglycerin, sunflower seed oil, rosemary leaf extract, and bisabolol; The phase F raw materials include: citric acid and deionized water; 2) Pretreatment: Place the phase B raw material in a container, heat it to 80-90°C, and stir until it is evenly dissolved to obtain pre-dissolved phase B; Place the phase D raw materials in another container, heat to 40-50°C, and stir until evenly dissolved to obtain pre-dissolved phase D; Place the raw materials of phase F in another container and stir until dissolved evenly to obtain pre-dissolved phase F; 3) Add all components in phase A into an emulsifying pot, heat to 75-85°C, and stir until they are evenly dispersed; 4) Put all the components in phase C into the oil phase pot, heat it to 75-85°C, then add the pre-dissolved phase B, stir and disperse until it is evenly dispersed; 5) Keep the temperature at 75-85℃, filter the materials in the oil phase pot into the emulsification pot, stir at 20-40 rpm for 10-30 minutes, and homogenize at 2800-3000 rpm until the emulsification is uniform; 6) Cool the emulsifier to 40-45°C, add the pre-dissolved phase D, stir at 20-40 rpm for 10-30 minutes, and homogenize at 1000-1200 rpm until evenly dispersed; 7) Keep the emulsifying pot at 40-45°C, add the F phase material, stir at 20-40 rpm, stir for 10-30 minutes, and homogenize at 1000-1200 rpm until evenly dispersed; 8) Cooling to below 38° C., stopping stirring, inspecting, filtering, and discharging to obtain the lipid encapsulation system.

3. The preparation method according to claim 2, characterized in that: In step 3), the mixture is stirred at 20-40 rpm for 10-30 minutes, and homogenized at 2000-3000 rpm for 5-20 minutes; in step 4), the mixture is stirred at 20-40 rpm for 10-30 minutes.

4. The preparation method according to claim 2, characterized in that: In the raw materials of phase A, the mass ratio of deionized water, sodium hyaluronate, glycerin, butylene glycol, ammonium acryloyldimethyltaurate / VP copolymer, and Tremella fuciformis fruiting body extract is 70.0445:0.1010:4.0000:1.0000:0.5000:0.0200.

5. The preparation method according to claim 2, characterized in that: In the phase B raw material, the mass ratio of ceramide NP, coconut oil alcohol caprylate / caprate, and caprylic / capric triglyceride is 0.1010:2.0000:1.5000.

6. The preparation method according to claim 2, characterized in that: Among the raw materials of phase C, the mass ratios of stearyl alcohol, dioctyldodecyl lauroyl glutamate, cetearyl olivate, sorbitan olivate, avocado butter, jojoba seed oil, phytosteryl oleate, phytosterols, sunflower seed oil, white beeswax, squalane, behenyl alcohol, and unsaponifiable matter of sunflower seed oil are 0.3000: 0.7000: 1.2000: 0.8000: 3.0000: 4.0000: 0.5910: 0.0090: 3.0000: 0.8000: 2.0000: 1.0000: 0.5000.

7. The preparation method according to claim 2, characterized in that: In the phase D raw material, the mass ratio of panthenol to deionized water is 0.8000:0.8000.

8. The preparation method according to claim 2, characterized in that: In the raw materials of phase E, the mass ratio of dipropylene glycol, raspberry ketone, caprylyl glycol, ethylhexylglycerin, sunflower seed oil, rosemary leaf extract, and bisabolol is 0.6500:0.2000:0.1200:0.0300:0.1045:0.0055:0.1010.

9. The preparation method according to claim 2, characterized in that: In the F phase raw material, the mass ratio of citric acid to deionized water is 0.0025:0.0200.

10. Application of a lipid encapsulation system in the preparation of a moisturizing cream product for infants and young children, characterized in that: The lipid-like encapsulation system is the lipid-like encapsulation system according to claim 1 or the lipid-like encapsulation system prepared according to the preparation method according to any one of claims 2-9.

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