A radio-stimulated surgical suture for promoting peripheral nerve healing, and its preparation method and application

Abstract

The present invention relates to the technical field of peripheral nerve injury suturing, and specifically discloses a radio-stimulated surgical suture for promoting peripheral nerve healing, and its preparation method and application. The radio-stimulated surgical suture comprises a Mo core layer and a PLGA coating. The preparation method of the radio-stimulated surgical suture is as follows: using metallic Mo to form the core layer, ejecting a PLGA spinning solution through a spinneret, and curing it in a coagulation bath to form a film, and forming a uniformly coated PLGA coating on the Mo core layer by wet spinning technology to obtain a radio-stimulated surgical suture for promoting peripheral nerve healing. The radio-stimulated surgical suture prepared by the present invention obtains energy from an external electromagnetic field through electromagnetic induction and capacitive coupling, and forms a stable electric field locally between the suture and the wound, thereby acting on the damaged tissue. The suture can promote neuronal axon growth, Schwann cell migration, etc., and improve the efficiency of nerve tissue regeneration.

Description

Technical Field

[0001] The present invention relates to the technical field of peripheral nerve injury suturing, and more particularly to a radio-stimulated surgical suture for promoting peripheral nerve healing, and a preparation method and application thereof. Background Art

[0002] Peripheral nerve injury (PNI) is a common clinical problem worldwide. Approximately one million people suffer peripheral nerve damage each year. This injury can be caused by a variety of factors, including traumatic accidents, tumor damage, and surgical injury. Although numerous studies have proposed various treatment options, clinical treatment for large lesions and long-segment peripheral nerve injuries remains a significant challenge. How to effectively repair these injuries and restore their function remains a major issue in global medicine.

[0003] In the human body, bioelectricity is a fundamental component of the organism. Cell membrane potential plays a crucial role in regulating the cell cycle, migration, proliferation, and differentiation. Action potentials determine cellular excitability and play a central role in nerve cells. Specifically, bioelectricity regulates a variety of biological processes, such as the exchange of information between synapses and neurons and the function of neural tissue cells. With the in-depth study of natural bioelectricity and biopiezoelectric phenomena, electroactive biomaterials, as an interdisciplinary subject at the intersection of molecular chemistry and physics, engineering materials, biology, and medicine, have gradually become a research hotspot. These materials, including biocompatible conductive materials and piezoelectric materials, have made significant progress in the design and preparation of biomaterial scaffolds. Studies have shown that low-frequency pulsed electrical stimulation can significantly promote the regeneration of peripheral nerve injuries. Providing electrical stimulation to promote nerve repair through these electroactive biomaterials has enormous application potential.

[0004] However, due to the diversity of PNI sites and the long functional reconstruction cycle, existing electrical stimulation devices and electrodes face many limitations in clinical applications and may cause serious complications, including inflammation, immune rejection, and pain. In addition, most existing electrical stimulation schemes rely on percutaneous electrode wires to transmit electrical stimulation signals to the injured area. This method not only has a high risk of infection, but also requires a second surgery to remove the implanted electrodes after tissue repair is completed, which greatly limits its clinical application.

[0005] To address these issues, researchers have proposed many innovative solutions, particularly in the development of wireless electrical stimulation systems. Currently, the exploration of wireless electrical stimulation systems that do not require external power supplies or percutaneous electrode leads has become a research hotspot. However, current wearable or implantable electrical stimulation systems still face numerous technical limitations. For example, most systems can only provide simple electrical pulse stimulation with fixed intensity and frequency, lacking the ability to synchronize with physiological states, thus affecting the repair effect. Furthermore, most of these electrical stimulation systems rely on specific energy supply devices. For example, the absorbable patch electrode reported by Lee et al. requires an external power supply. To address this shortcoming, Kim et al. adopted a noninvasive approach, developing a patch electrode that uses ultrasound to transmit vibrations, thereby generating electrical stimulation through the principle of triboelectricity. However, during treatment, an ultrasound probe is still required to power the device at the wound site, thus not fundamentally resolving these issues. These issues limit their application in neural function reconstruction.

[0006] Patent publication number CN110170068A discloses a multifunctional medical suture and its preparation method, comprising a suture body, a multi-layer adhesive coating, and drug-loaded nanoparticles. By loading sustained-release nanoparticles with various drugs, this medical suture can enhance the healing of tissues such as tendons, muscles, and epidermis, while also effectively suppressing early inflammatory responses and alleviating pain later in the injury. However, for suturing nerve tissue, this suture cannot provide a stable drug release concentration for tissue healing, nor can it provide the electrical cues necessary to guide the regeneration process, making it difficult to increase the regeneration rate of peripheral nerves after injury. Summary of the Invention

[0007] In order to solve the shortcomings of existing medical sutures used for suturing nerve tissue, such as being unable to provide a stable drug release concentration for tissue healing, and being unable to provide the electrical clue stimulation necessary for the regeneration process of nerve tissue, and being difficult to increase the regeneration rate after peripheral nerve injury, the present invention provides a radio-stimulated surgical suture for promoting peripheral nerve healing, and its preparation method and application. The suture comprises a Mo core layer and a PLGA coating, which can not only provide electrical stimulation to promote tissue healing, but also gradually degrade in the body without the need for additional removal. The core working principle of the suture of the present invention is to utilize the difference in dielectric constant between the suture and human tissue, and under the action of an external electromagnetic field (such as the electric field released by electronic devices such as mobile phones and computers), form an electric potential difference between the suture and human tissue, thereby generating controllable electrical stimulation to promote the healing of nerve tissue.

[0008] The present invention provides a radio-stimulated surgical suture for promoting peripheral nerve healing, which adopts the following technical solutions:

[0009] A radio frequency stimulation surgical suture for promoting peripheral nerve healing comprises a Mo core layer and a PLGA coating.

[0010] Preferably, the radio stimulation principle is the human body coupling principle of the external electromagnetic field.

[0011] Preferably, the Mo core layer is used to provide electrical stimulation and improve the mechanical strength and electrical conductivity of the suture.

[0012] Preferably, the PLGA coating is used to enhance biocompatibility, reduce inflammatory responses caused by metal ion release, and regulate the degradation rate of the suture.

[0013] A method for preparing a radio-stimulated surgical suture for promoting peripheral nerve healing comprises the following steps:

[0014] Metal Mo filament is used as the Mo core layer, PLGA spinning solution is ejected through a spinneret and solidified into a film in a coagulation bath, and a uniformly coated PLGA coating is formed on the Mo core layer through wet spinning technology to obtain a wireless radio stimulation surgical suture for promoting peripheral nerve healing.

[0015] Preferably, the PLGA spinning solution is prepared by mixing PLGA and hexafluoroisopropanol.

[0016] Preferably, the mass fraction of the PLGA spinning solution is 4-20%.

[0017] Preferably, the mass fraction of the PLGA spinning solution is 12%.

[0018] Preferably, the diameter of the radiofrequency stimulation surgical suture for promoting peripheral nerve healing is 50-200 μm, which is suitable for microsurgical suturing procedures.

[0019] Preferably, the diameter of the Mo core layer is 30-70 μm.

[0020] Preferably, the diameter of the Mo core layer is 50 μm.

[0021] Preferably, the process parameters of the wet spinning are as follows: single-hole nozzle, pore size 140-160 μm, and drafting speed 5-8 r / min.

[0022] Preferably, the process parameters of the wet spinning are as follows: single-hole nozzle, pore size 150 μm, and drawing speed 6 r / min.

[0023] Preferably, the coagulation bath is 90-98% ethanol, and the draw ratio is 1-1.2.

[0024] Preferably, the coagulation bath is 95% ethanol and the draw ratio is 1.

[0025] Application of a radio-stimulated surgical suture for promoting peripheral nerve healing in the repair of peripheral nerve injuries.

[0026] Preferably, the application method is as follows: using radio stimulation surgical sutures for promoting peripheral nerve healing to suture the damaged part of the peripheral nerve, and then providing electrical stimulation under the action of an external electromagnetic field to promote the recovery and regeneration of nerve tissue.

[0027] Preferably, the peripheral nerve injuries include facial nerve, brachial plexus, sciatic nerve, etc.

[0028] Preferably, the process of achieving radio stimulation in vivo by the radio stimulation surgical suture for promoting peripheral nerve healing is to generate 2-8V electrical stimulation under the action of an external electromagnetic field to promote nerve axon growth, myelin regeneration and neurovascular regeneration.

[0029] In summary, the present invention has the following beneficial effects:

[0030] The radio-stimulation surgical sutures prepared by the present invention for promoting peripheral nerve healing obtain energy from an external electromagnetic field through electromagnetic induction and capacitive coupling, and form a stable electric field locally on the suture and wound, thereby acting on the damaged tissue. The sutures of the present invention can promote the growth of neuronal axons, the migration of Schwann cells, etc., and improve the efficiency of nerve tissue regeneration. Compared with traditional implantable electrical stimulation devices, the human-coupled radio-stimulation surgical sutures of the present invention do not require an additional implantable power supply, and can provide energy only through an external electromagnetic field (such as the electric field released by electronic devices such as mobile phones and computers), which simplifies the clinical application process and has broad application prospects in peripheral nerve repair.

[0031] The present invention utilizes Mo's excellent conductivity, good mechanical properties, and reliable biosafety to improve the transmission efficiency of electrical stimulation and enhance the electrical regulation of nerves or other tissues. Mo has high strength and toughness. Compared with other degradable metals (such as magnesium), Mo has more stable mechanical properties and is less prone to breakage or damage, which helps maintain the structural integrity and long-term stability of radiofrequency stimulation surgical sutures. Low doses of Mo element have good biocompatibility.

[0032] The present invention utilizes PLGA coating to improve biocompatibility, reduce inflammatory reactions that may be caused by metal ions, regulate the degradation rate of Mo, reduce the potential toxicity caused by metal release, enhance mechanical strength, reduce the risk of suture breakage, improve electrical stimulation stability, and optimize the function of electrical stimulation surgical sutures. The surgical sutures of the present invention can naturally degrade after completing the repair task, avoiding secondary surgery for removal. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1This is a schematic diagram of the principle of the radio-stimulation surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention generating electrical stimulation to human tissue under the action of an external electromagnetic field.

[0034] Figure 2 This is a principle circuit diagram of the radio-stimulation surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention, which generates electrical stimulation to human tissue under the action of an external electromagnetic field.

[0035] Figure 3 Schematic diagram of the radio-stimulated surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention.

[0036] Figure 4 This is a diagram of the surgical process of using the radio-stimulated surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention for peripheral nerve suturing.

[0037] Figure 5 These are the results of sampling, HE staining, and toluidine blue staining of the facial nerve of a rat sutured with surgical sutures prepared in Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 of the present invention.

[0038] Figure 6 These are immunofluorescence images of structural protein expression in rat facial nerves sutured with surgical sutures prepared in Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 of the present invention.

[0039] Figure 7 This is a graph showing the effect of the radio-stimulated surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention on the survival rate of cells cultured in vitro.

[0040] Figure 8 This is a comparison of HE staining images of the main organs of rats and healthy rats after the radio-frequency stimulation surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention was used in rats.

[0041] Figure 9 This is a graph showing the range of voltage generated in vitro by the radio-stimulated surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention as a function of the distance from the electromagnetic field emission source.

[0042] Figure 10 This is a graph showing the change trend of the voltage generated by the radio-stimulated surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention during its degradation in vivo as a function of the degradation time.

[0043] Figure 11 This is a comparison chart of the maximum load-bearing capacity of the radio-stimulated surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention and commercially available surgical sutures under static tensile conditions.

[0044] Figure 12 This is a comparison chart of the stiffness of the radio-stimulated surgical suture for promoting peripheral nerve healing prepared in Example 1 of the present invention and commercially available surgical sutures. DETAILED DESCRIPTION

[0045] Refer to the attached Figure 1-12 The present invention will be further described in detail below with reference to the embodiments.

[0046] The sources of raw materials used in the examples of the present invention are as follows:

[0047] Metal Mo is molybdenum wire purchased from Wuxi Shengshida New Materials Co., Ltd.

[0048] Poly(lactic-co-glycolic acid) (PLGA) was purchased from Nature Works, USA.

[0049] Hexafluoroisopropanol was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0050] Ethanol was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0051] Commercially available surgical sutures (suture needles with thread; models: O3 / 8, 9-0) were purchased from Ningbo Medical Suture Needle Co., Ltd.

[0052] Commercially available nylon suture (9-0) was purchased from Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.

[0053] Commercially available absorbable suture samples (9-0) were purchased from Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.

[0054] Example 1

[0055] A radio-stimulation surgical suture for promoting peripheral nerve healing, comprising a Mo core layer and a PLGA coating;

[0056] A method for preparing a radio-stimulated surgical suture for promoting peripheral nerve healing comprises the following steps:

[0057] At room temperature, PLGA and hexafluoroisopropanol were mixed to prepare a PLGA spinning solution with a mass fraction of 12%;

[0058] Using molybdenum wire as the Mo core layer, the PLGA spinning solution was ejected through a spinneret and solidified into a film in a coagulation bath. A uniform PLGA coating was formed on the Mo core layer by wet spinning technology, resulting in a 120 μm diameter wireless radio-stimulated surgical suture for promoting peripheral nerve healing.

[0059] The wet spinning process parameters are as follows: Czochralski wet spinning, using a glass cavity with an interchangeable spinning head as a container, pouring the biodegradable polymer PLGA spinning solution into the glass cavity and fixing it on the spinning table, the Mo core layer entering from the top and being drawn out from the metal spinning needle at the bottom of the cavity, a single-hole nozzle with an aperture of 150 μm, room temperature, and a drawing speed of 6 r / min.

[0060] The coagulation bath was 95% ethanol. The drawn molybdenum wire was treated in the coagulation bath and then cleaned in a room temperature water bath. The drawing ratio was 1.

[0061] A method for applying a radio-stimulated surgical suture for promoting peripheral nerve healing is as follows:

[0062] The rat facial nerve transection model was established by severing the main trunk of the rat facial nerve. The transected facial nerve of the rat was sutured using radio-induced sutures. The rats were exposed to an adjustable electromagnetic field for 2 hours daily, allowing the sutures to electrically stimulate the transected nerve of the rat to promote nerve tissue growth.

[0063] Example 2

[0064] A radio-stimulation surgical suture for promoting peripheral nerve healing, comprising a Mo core layer and a PLGA coating;

[0065] A method for preparing a radio-stimulated surgical suture for promoting peripheral nerve healing comprises the following steps:

[0066] At room temperature, PLGA and hexafluoroisopropanol were mixed to prepare a PLGA spinning solution with a mass fraction of 4%;

[0067] Using molybdenum wire as the Mo core layer, the PLGA spinning solution was ejected through a spinneret and solidified into a film in a coagulation bath. A uniform PLGA coating was formed on the Mo core layer by wet spinning technology, resulting in a 100 μm diameter wireless radio-stimulated surgical suture for promoting peripheral nerve healing.

[0068] The wet spinning process parameters are as follows: Czochralski wet spinning, using a glass cavity with an interchangeable spinning head as a container, pouring the biodegradable polymer PLGA spinning solution into the glass cavity and fixing it on the spinning table, the Mo core layer entering from the top and being drawn out from the metal spinning needle at the bottom of the cavity, a single-hole nozzle with an aperture of 150 μm, room temperature, and a drawing speed of 8 r / min.

[0069] The coagulation bath was 90% ethanol. The drawn molybdenum wire was treated in the coagulation bath and then cleaned in a room temperature water bath. The drawing ratio was 1.2.

[0070] Example 3

[0071] A radio-stimulation surgical suture for promoting peripheral nerve healing, comprising a Mo core layer and a PLGA coating;

[0072] A method for preparing a radio-stimulated surgical suture for promoting peripheral nerve healing comprises the following steps:

[0073] At room temperature, PLGA and hexafluoroisopropanol were mixed to prepare a PLGA spinning solution with a mass fraction of 20%.

[0074] Using molybdenum wire as the Mo core layer, the PLGA spinning solution was ejected through a spinneret and solidified into a film in a coagulation bath. A uniform PLGA coating was formed on the Mo core layer by wet spinning technology, resulting in a 150 μm diameter wireless radio-stimulated surgical suture for promoting peripheral nerve healing.

[0075] The wet spinning process parameters are as follows: Czochralski wet spinning, using a glass cavity with an interchangeable spinning head as a container, pouring the biodegradable polymer PLGA spinning solution into the glass cavity and fixing it on the spinning table, the Mo core layer entering from the top and being drawn out from the metal spinning needle at the bottom of the cavity, a single-hole nozzle with an aperture of 150 μm, room temperature, and a drawing speed of 5 r / min.

[0076] The coagulation bath was 98% ethanol. The drawn molybdenum wire was treated in the coagulation bath and then cleaned in a room temperature water bath. The drawing ratio was 1.1.

[0077] Comparative Example 1

[0078] A method for applying a radio-stimulated surgical suture for promoting peripheral nerve healing is as follows:

[0079] The transected facial nerve of rats was sutured using the radio-frequency stimulation surgical suture for promoting peripheral nerve healing in Example 1. The rats were raised under conventional conditions without being exposed to electromagnetic fields.

[0080] Comparative Example 2

[0081] A method for applying commercially available surgical sutures is as follows:

[0082] The transected facial nerve of the rats was sutured using commercially available surgical sutures (suture needles with thread; models: O3 / 8, 9-0; purchased from Ningbo Medical Suture Needle Co., Ltd.). The rats were housed under conventional conditions and were not exposed to electromagnetic fields.

[0083] Comparative Example 3

[0084] A method for applying commercially available surgical sutures is as follows:

[0085] The transected facial nerves of rats were sutured using commercially available surgical sutures (suture needles with thread; models: O3 / 8, 9-0; purchased from Ningbo Medical Suture Needle Co., Ltd.), and the rats were exposed to an adjustable electromagnetic field for 2 h daily for electromagnetic field exposure.

[0086] Experiment 1: Effect of radio-stimulated surgical sutures on the healing of transverse nerve tissue in rats

[0087] The healing of the cross-section nerve tissue of the rats treated with the sutures in Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3 was observed after 1 month, and the expression of the main structural and functional proteins was observed. The specific results are shown in Table 1 and the attached Figure 5-6 .

[0088] Table 1:

[0089]

[0090] From Table 1 and Appendix Figure 5-6 The data show that: after regular exposure to electromagnetic fields for electrical stimulation treatment, the transected facial nerves of rats were sutured using the radio-stimulated surgical sutures for promoting peripheral nerve healing in Example 1 of the present invention. The recovery rate of nerve tissue was significantly accelerated, the maturity of regenerated nerve tissue was higher, and the therapeutic effect of facial nerve injury in rats after treatment was good. In Comparative Example 1, the transected facial nerves of rats were sutured using the radio-stimulated surgical sutures for promoting peripheral nerve healing in Example 1. However, since the rats were not exposed to electromagnetic fields, the electrical stimulation treatment effect could not be produced on the wound, the nerve maturity was poor, and the final therapeutic effect was poor. In Comparative Example 2, commercially available surgical sutures (suture needles with thread; model: O3 / 8, 9-0; purchased from Ningbo Medical Suture Needle Co., Ltd.) were used to suture the transected facial nerve of rats. In Comparative Example 3, commercially available surgical sutures (suture needles with thread; model: O3 / 8, 9-0; purchased from Ningbo Medical Suture Needle Co., Ltd.) were used to suture the transected facial nerve of rats and an electric field was applied. The experimental results showed that the tissue recovery effect and neuronal maturity of Comparative Examples 2 and 3 were not as good as those in Example 1, indicating that Comparative Examples 2 and 3 could not generate electrical stimulation treatment at the wound, so the nerve tissue regeneration was slow and the therapeutic effect was not good.

[0091] Attachment Figure 5 HE staining of nerve tissue sections showed that compared with the different treatment groups, the nerve tissue in Example 1 was less inflamed, the nerve tissue area accounted for a higher proportion, and the nerve fibers were arranged more neatly and orderly, while the other treatment groups had obvious inflammation at the nerve transection site, a smaller proportion of nerve tissue area, and disordered arrangement of nerve fibers.

[0092] Experiment 2: Effect of radiofrequency stimulation of surgical sutures on the survival rate of cells cultured in vitro

[0093] PC12 cells were cultured in vitro, and the radio-stimulated surgical suture for promoting peripheral nerve healing in Example 1 was added to the cell culture medium. The mass ratio of radio-stimulated surgical suture to culture medium was controlled to be 1:9. After culturing for 24 hours, the culture medium was discarded, and live and dead cells were stained using a cell viability kit. The live / dead cell ratio was observed using a fluorescence microscope. The specific results are shown in the attached Figure 7 .

[0094] By the attached Figure 7 It can be seen that after co-culturing cells with radio-stimulated surgical sutures, the number of dead cells (red) is very low compared to the number of living cells (green).

[0095] Experiment 3: Comparison of HE staining images of the main organs of rats treated with radio-stimulated surgical sutures and healthy rats without any treatment

[0096] Two months after the facial nerve of rats was transected using radio-stimulation surgical sutures, the rats were killed and their hearts, livers, spleens, lungs, and kidneys were removed. After fixation with paraformaldehyde, paraffin sections were prepared and HE-stained. The sections were compared with the HE-stained sections of the main organs of healthy rats without any treatment to observe whether the metabolites of radio-stimulation surgical sutures in the body would be toxic to the main organs of the rats. The specific results are shown in the attached Figure 8 .

[0097] By the attached Figure 8 It can be seen that the main organs of rats treated with facial nerve transection surgery using radiofrequency stimulation did not show inflammation or other lesions, and the organ structure was basically consistent with that of healthy rats.

[0098] Experiment 4: The influence of the voltage generated by radio-stimulated surgical sutures in vitro and their distance from the electromagnetic field emission source

[0099] Prepare several samples of radio-stimulated surgical sutures. Use an electromagnetic field generator with a stable output frequency as the magnetic field source. Suture samples were sewn into rats, leaving a section of suture for connection to an oscilloscope. The rats were placed at 10 cm, 20 cm, 30 cm, 40 cm, and 50 cm from the transmitter. The peak voltage induced on the suture at each distance was recorded using the oscilloscope. Each experiment was repeated three times, and the mean value was taken. See the attached data for specific results. Figure 9 .

[0100] By the attached Figure 9 It can be seen that the voltage induced by the radio stimulation suture gradually decreases as the distance from the electromagnetic field source increases. Figure 9The results showed that the maximum voltage (approximately 7.8 V) could be sensed on the suture at a distance of 10 cm from the transmitter, while the induced voltage dropped to a minimum (approximately 1.6 V) at a distance of 50 cm. This result suggests that wireless stimulation sutures have significant sensing response within 50 cm and are suitable for clinical tissue suturing environments with a certain depth.

[0101] Experiment 5: Effect of the Voltage and Degradation Time Generated by Radio-Stimulated Surgical Sutures During Degradation in Vivo

[0102] The radio-stimulated sutures were implanted in the subcutaneous tissue of rats. At different time points over 70 days, the animals were anesthetized and the suture residue was exposed. The sutures were excited by an external electromagnetic field. Electrodes were connected to both ends of the sutures, and the voltage generated by the sutures was recorded using an oscilloscope. The peak value of the induced voltage was recorded and compared with the initial value. The specific results are shown in the attached figure. Figure 10 .

[0103] By the attached Figure 10 It can be seen that: as the implantation time increases, the suture induced voltage does not change significantly compared with the initial level, suggesting that its metabolic rate is slower than the nerve healing rate, which can ensure a stable therapeutic effect during the nerve recovery period.

[0104] Experiment 6: Comparison of Mechanical Properties of Radio-Stimulated Surgical Sutures and Commercially Available Surgical Sutures

[0105] Prepare samples of radio-stimulated surgical sutures, commercially available nylon sutures (models: O3 / 8, 9-0, Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.), and commercially available absorbable sutures (models: O3 / 8, 9-0, Shanghai Pudong Jinhuan Medical Supplies Co., Ltd.). Tensile tests were performed using an electronic tensile tester. The maximum tensile strength, elongation at break, and elastic modulus of each suture were recorded. Detailed results are shown in the attached table. Figure 11-12 .

[0106] By the attached Figure 11-12 It can be seen that: Figure 11 The results showed that the maximum tensile strength of radio-stimulated surgical sutures was significantly higher than that of two commercially available sutures, indicating that they have good mechanical support during the suturing process. Figure 12 Further results showed that its elastic modulus and elongation at break were comparable to those of two commercially available sutures, demonstrating good toughness and tensile adaptability. Overall, the results suggest that the embedded conductive component in the suture does not affect its basic mechanical properties, making it suitable for clinical suture use.

[0107] This specific embodiment is merely an explanation of the present invention and is not intended to limit the present invention. After reading this specification, those skilled in the art may make non-creative modifications to this embodiment as needed. However, as long as such modifications are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A radio-stimulated surgical suture for promoting peripheral nerve healing, characterized in that: It comprises a Mo core layer and a PLGA coating, wherein the Mo core layer has a diameter of 30-70 μm, and the PLGA coating is formed by uniformly coating the Mo core layer with PLGA spinning solution through wet spinning technology; The radio-stimulation surgical suture for promoting peripheral nerve healing is used to suture the damaged part of the peripheral nerve, and then provides electrical stimulation under the action of an external electromagnetic field to promote the recovery and regeneration of nerve tissue.

2. A method for preparing a radio-assisted surgical suture for promoting peripheral nerve healing according to claim 1, characterized in that: The following steps are involved: Metal Mo is used as the Mo core layer, the PLGA spinning solution is ejected through a spinneret and solidified into a film in a coagulation bath. A uniformly coated PLGA coating is formed on the Mo core layer through wet spinning technology to obtain a wireless radio-stimulated surgical suture for promoting peripheral nerve healing.

3. The method for preparing a radio-stimulated surgical suture for promoting peripheral nerve healing according to claim 2, wherein: The PLGA spinning solution is prepared by mixing PLGA and hexafluoroisopropanol; the mass fraction of the PLGA spinning solution is 4-20%.

4. The method for preparing a radio-assisted surgical suture for promoting peripheral nerve healing according to claim 2, wherein: The diameter of the radio frequency stimulation surgical suture for promoting peripheral nerve healing is 50-200 μm.

5. The method for preparing a radio-assisted surgical suture for promoting peripheral nerve healing according to claim 2, wherein: The process parameters of the wet spinning are as follows: single-hole nozzle, hole diameter 140-160 μm, and drafting speed 5-8 r / min.

6. The method for preparing a radio-assisted surgical suture for promoting peripheral nerve healing according to claim 2, wherein: The coagulation bath contains 90-98% ethanol by volume, and the draft ratio is 1-1.

2.

7. A use of the radio-stimulated surgical suture for promoting peripheral nerve healing according to claim 1, characterized in that: Used for repair of peripheral nerve damage.

8. The use of a radio-stimulated surgical suture for promoting peripheral nerve healing according to claim 7, characterized in that: The peripheral nerve injuries include facial nerve, brachial plexus and sciatic nerve.

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