Extraction device and application

Abstract

The invention provides an extraction device and application, the extraction device comprises a negative pressure assembly and a positive pressure assembly, the negative pressure assembly is used for applying negative pressure to an outlet end of a filter container, and the positive pressure assembly is used for applying positive pressure to an inlet end of the filter container. By arranging the negative pressure assembly and the positive pressure assembly, the extraction device can flexibly change the pressure applying mode according to different requirements of different links in the product extraction process, so that the use effect of the extraction device is improved. The invention relates to the field of product extraction.

Description

Technical Field

[0001] The present application relates to the field of product extraction, in particular to an extraction device and its application. Background Art

[0002] There is a need to separate products and reaction solutions in biological experiments. For example, after generating nucleic acids through an enzymatic reaction, it is necessary to extract the products from the mixture for the next experimental process, such as generating linearized DNA from circular DNA digestion and then generating RNA from in vitro transcription of linearized DNA.

[0003] Generally, products can be extracted by centrifugation and magnetic bead methods. However, the centrifugation method has the problem of being unsuitable for batch preparation, while the magnetic bead method has the problem of high cost. To balance low cost and batch production, some extraction devices in related technologies use the method of applying pressure to clean impurities and elute products.

[0004] However, the current extraction devices have relatively single functions and are difficult to meet the differentiated requirements of the impurity cleaning process and the product elution process, resulting in less than ideal use effects.

[0005] Specifically, in some related technologies, positive pressure is applied for cleaning and elution. When pressurizing, the pressure source needs to be connected to the inlet of the container. However, during the cleaning process, it is necessary to repeatedly add cleaning liquid to the container. Therefore, the pressure source and the container need to be repeatedly connected and disconnected, which not only reduces the cleaning efficiency but also increases the wear of the connection part.

[0006] In some related technologies, negative pressure is applied for cleaning and elution. When pressurizing, the pressure source needs to be connected to the outlet of the container. However, during the elution process, the products are also discharged from the outlet, and some products may be sucked away by the pressure source, resulting in a reduction in the collected products and a decrease in experimental efficiency. Summary of the Invention

[0007] The present application aims to solve at least one of the technical problems existing in the prior art. For this purpose, the present application provides an extraction device and its application. The extraction device can apply pressure to the filtration container in different ways, thereby improving the use effect of the extraction device.

[0008] According to the extraction device provided by the present application, it includes a negative pressure component and a positive pressure component. The negative pressure component is used to apply negative pressure to the outlet end of the filtration container, and the positive pressure component is used to apply positive pressure to the inlet end of the filtration container.

[0009] According to the extraction device provided by the present application, it has at least the following technical effects: By configuring the negative pressure component and the positive pressure component, the extraction device can flexibly change the way of applying pressure according to the differentiated requirements of different links in the product extraction process, thereby improving the use effect of the extraction device.

[0010] According to some embodiments of the present application, the extraction device includes a first tray, and a plurality of first placement holes are formed in the first tray, and the first placement holes are used for placing the filter container.

[0011] According to some embodiments of the present application, the negative pressure assembly is formed with a negative pressure hole, and the negative pressure hole is used for accommodating the outlet end of the filter container.

[0012] According to some embodiments of the present application, the first placement holes penetrate through the first tray, the depth of the first placement holes is less than the height of the filter container, and the outlet end of the filter container penetrates out of the first tray to extend into the negative pressure hole.

[0013] According to some embodiments of the present application, the diameter of the negative pressure hole is greater than the diameter of the part of the filter container inserted into the negative pressure hole.

[0014] According to some embodiments of the present application, at least one of the negative pressure assembly and the first tray includes a first positioning structure, and the first positioning structure is used for positioning the relative positions of the negative pressure assembly and the first tray.

[0015] According to some embodiments of the present application, the negative pressure assembly is formed with a first receiving groove, the first tray can be embedded in the first receiving groove, and the side wall of the first receiving groove serves as the first positioning structure.

[0016] According to some embodiments of the present application, the negative pressure assembly is formed with a first air distribution chamber, and each of the negative pressure holes is communicated with the first air distribution chamber, and the first air distribution chamber is used for communicating with an external air source.

[0017] According to some embodiments of the present application, the negative pressure assembly includes a first seat body and a second seat body, the second seat body is detachably arranged on the first seat body, the negative pressure hole is located in the second seat body, and the second seat body and the first seat body surround and define the first air distribution chamber.

[0018] According to some embodiments of the present application, at least one of the first seat body and the second seat body includes a second positioning structure, and the second positioning structure is used for positioning the relative positions of the first seat body and the second seat body.

[0019] According to some embodiments of the present application, the first seat body is formed with a second receiving groove, the second seat body can be embedded in the second receiving groove, and the side wall of the second receiving groove serves as the second positioning structure.

[0020] According to some embodiments of the present application, the negative pressure assembly includes a first sealing member, and the first sealing member is arranged between the first seat body and the second seat body to seal the connection part between the first seat body and the second seat body.

[0021] According to some embodiments of the present application, the extraction device includes a second tray for carrying the first tray. The second tray is formed with a plurality of second placement holes for placing a collection container, and the collection container is used to receive the liquid from the filtration container.

[0022] According to some embodiments of the present application, the first placement hole penetrates through the first tray, and the depth of the first placement hole is less than the height of the filtration container. The outlet end of the filtration container penetrates through the first tray to extend into the collection container.

[0023] According to some embodiments of the present application, at least one of the second tray and the first tray includes a third positioning structure for positioning the relative positions of the second tray and the first tray.

[0024] According to some embodiments of the present application, the second tray is formed with a third receiving groove into which the first tray can be inserted, and the side wall of the third receiving groove serves as the third positioning structure.

[0025] According to some embodiments of the present application, at the bottom of the first tray, the diameter of the second placement hole is greater than the diameter of the collection container to avoid the collection container.

[0026] According to some embodiments of the present application, the second placement hole penetrates through the second tray, and the depth of the second placement hole is less than the height of the collection container, and the collection container penetrates through the second tray.

[0027] According to some embodiments of the present application, the negative pressure assembly is formed with a negative pressure hole for accommodating the collection container that penetrates through the second tray, and the diameter of the negative pressure hole is greater than the diameter of the part of the collection container inserted into the negative pressure hole.

[0028] According to some embodiments of the present application, there is a first gap between the outer wall of the filtration container and the inner wall of the collection container, a second gap between the outer wall of the collection container and the inner wall of the second placement hole, and a third gap between the outer wall of the collection container and the inner wall of the negative pressure hole. The filtration container, the collection container, the first gap, the second gap, and the third gap are sequentially connected to form a negative pressure elution gas path.

[0029] According to some embodiments of the present application, there is a fourth gap between the outer wall of the collection container and the inner wall of the first placement hole, and the fourth gap communicates with the negative pressure elution gas path.

[0030] According to some embodiments of the present application, the negative pressure assembly includes a first positioning structure for positioning the relative positions of the negative pressure assembly and the second tray.

[0031] According to some embodiments of the present application, the positive pressure assembly is formed with positive pressure holes for communicating with the inlet end of the filtration container.

[0032] According to some embodiments of the present application, the positive pressure holes, the filtration container, and the collection container are sequentially communicated to form a positive pressure elution gas path.

[0033] According to some embodiments of the present application, the extraction device further has a negative pressure elution gas path, which coincides with the positive pressure elution gas path in the filtration container and the collection container.

[0034] According to some embodiments of the present application, at least one of the positive pressure assembly and the first tray includes a fourth positioning structure for positioning the relative positions of the positive pressure assembly and the first tray.

[0035] According to some embodiments of the present application, the positive pressure assembly is formed with a fourth receiving groove into which the first tray can be inserted, and the side wall of the fourth receiving groove serves as the fourth positioning structure.

[0036] According to some embodiments of the present application, the extraction device includes a second seal disposed between the positive pressure assembly and the first tray for sealing the connection portion between the positive pressure assembly and the first tray.

[0037] According to some embodiments of the present application, an annular seal groove is formed on the outer periphery of each of the positive pressure holes, and the second seal is disposed in the seal groove.

[0038] According to some embodiments of the present application, the positive pressure assembly is formed with a second gas distribution chamber, and each of the positive pressure holes is communicated with the second gas distribution chamber, and the second gas distribution chamber is used for communicating with an external gas source.

[0039] According to some embodiments of the present application, the positive pressure assembly includes a first cover and a second cover, the second cover is detachably disposed on the first cover, the positive pressure holes are located on the second cover, and the second cover and the first cover enclose and define the second gas distribution chamber.

[0040] According to some embodiments of the present application, at least one of the first cover and the second cover includes a fifth positioning structure for positioning the relative positions of the first cover and the second cover.

[0041] According to some embodiments of the present application, a fifth receiving groove is formed on the first cover body, the second cover body can be embedded in the fifth receiving groove, and the side wall of the fifth receiving groove serves as the fifth positioning structure.

[0042] According to some embodiments of the present application, the positive pressure assembly includes a third seal, which is disposed between the first cover body and the second cover body to seal a connection portion between the first cover body and the second cover body.

[0043] The extraction platform provided in the present application includes the extraction device provided in the present application.

[0044] According to some embodiments of the present application, the extraction platform also includes a movable seat and a first driving member, the negative pressure assembly is arranged on the movable seat, and the first driving member can drive the movable seat to move horizontally so that the negative pressure assembly is aligned or staggered with the positive pressure assembly in the vertical direction.

[0045] According to some embodiments of the present application, the extraction platform further includes a position sensor, which is used to detect the position of the negative pressure component.

[0046] According to some embodiments of the present application, the extraction platform further includes a second driving member, and the second driving member is used to drive the positive pressure component to approach or move away from the negative pressure component.

[0047] According to some embodiments of the present application, the extraction platform further includes a guide member extending in a vertical direction, and the positive pressure assembly is connected to the guide member.

[0048] The product extraction method provided by the present application comprises:

[0049] Applying negative pressure to the filter container into which the cleaning liquid is added to perform cleaning;

[0050] Positive pressure is applied to the filtration container into which the eluent is added to perform elution.

[0051] According to some embodiments of the present application, negative pressure is applied to a filter container to which a cleaning liquid is added, comprising: placing the filter container in a first tray; placing the first tray in a negative pressure assembly; adding the cleaning liquid to the filter container; and applying negative pressure to an outlet end of the filter container by a negative pressure hole of the first tray.

[0052] According to some embodiments of the present application, positive pressure is applied to the filter container to which the eluent is added, comprising: placing the collecting container in a second tray; placing the second tray on the negative pressure assembly; placing the first tray on the second tray; adding the eluent to the filter container; pressing the first tray with the positive pressure assembly; and applying positive pressure to the inlet end of the filter container through the positive pressure hole of the positive pressure assembly.

[0053] The product extraction method provided by the present application includes:

[0054] Applying negative pressure to the filtration container added with a cleaning solution for cleaning;

[0055] Placing the filtration container in a collection container, and applying negative pressure to the filtration container added with an eluent for elution.

[0056] According to some embodiments of the present application, placing the filtration container in a collection container and applying negative pressure to the filtration container added with an eluent includes: putting the filtration container into a first tray; putting the collection container into a second tray; placing the second tray on the negative pressure assembly; placing the first tray on the second tray; adding the eluent to the filtration container; applying negative pressure to the outlet end of the filtration container through the negative pressure holes of the first tray.

[0057] According to some embodiments of the present application, after adding the eluent to the filtration container, the product extraction method includes: pressing the first tray with a positive pressure assembly; applying positive pressure to the inlet end of the filtration container through the positive pressure holes of the positive pressure assembly.

[0058] The extraction platform provided by the present application includes the extraction device provided by the present application. The product extraction method provided by the present application can be implemented based on the extraction device provided by the present application. Therefore, the experimental equipment and the product extraction method correspondingly have the beneficial effects provided by the extraction device, which will not be elaborated herein. Description of the Drawings

[0059] The drawings are used to provide a further understanding of the technical solutions disclosed in the present application, and constitute a part of the specification. Together with the embodiments disclosed in the present application, they are used to explain the technical solutions of the present disclosure, and do not constitute a limitation to the technical solutions disclosed in the present application.

[0060] Figure 1 It is a schematic structural diagram of the extraction device according to the embodiment of the present application;

[0061] Figure 2 It is a schematic connection diagram of the extraction device according to the embodiment of the present application when applying negative pressure;

[0062] Figure 3 It is an explosion diagram of the extraction device according to the embodiment of the present application when applying negative pressure;

[0063] Figure 4 It is a sectional view of the extraction device according to the embodiment of the present application when applying negative pressure;

[0064] Figure 5 It is a schematic connection diagram of the extraction device according to the embodiment of the present application when applying positive pressure;

[0065] Figure 6 It is a schematic diagram of an explosion when the extraction device of the embodiment of the present application applies positive pressure;

[0066] Figure 7 It is a schematic cross-sectional view of the extraction device of the embodiment of the present application when applying positive pressure;

[0067] Figure 8 It is a schematic cross-sectional view of the extraction device of the embodiment of the present application when applying negative pressure for elution;

[0068] Figure 9 is Figure 8 a partial enlarged view of part A of

[0069] Figure 10 It is a schematic cross-sectional view of the extraction device of the embodiment of the present application when applying negative pressure and positive pressure simultaneously for elution;

[0070] Figure 11 It is a schematic diagram of a partial structure of the extraction platform of the embodiment of the present application, which shows the installation relationship between the extraction device and the movable seat.

[0071] Reference numerals:

[0072] First tray 1100, first placement hole 1110, stepped hole 1111;

[0073] Second tray 1200, second placement hole 1210, third positioning structure 1220;

[0074] Negative pressure assembly 1300, second seat body 1310, negative pressure hole 1311, first positioning structure 1312, first seat body 1320, second positioning structure 1321, first gas distribution chamber 1330, first seal 1340;

[0075] Positive pressure assembly 1400, second cover body 1410, positive pressure hole 1411, fourth positioning structure 1412, first cover body 1420, fifth positioning structure 1421, second gas distribution chamber 1430, third seal 1440;

[0076] Second seal 1510;

[0077] Movable seat 1610, first driving member 1620, position sensor 1630;

[0078] Second driving member 1710, guiding member 1720;

[0079] First gap 1810, second gap 1820, third gap 1830, fourth gap 1840;

[0080] Filter container 1910, collection container 1920, flanging structure 1921. Detailed implementation manners

[0081] The embodiments of the present application will be described in detail below. The examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain the present application, and should not be construed as a limitation to the present application.

[0082] In the description of the present application, it should be understood that the orientation or positional relationship indicated by terms such as up, down, front, back, left, right, etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0083] In the description of the present application, the meaning of "several" is one or more, the meaning of "multiple" is more than two, and understandings such as "greater than", "less than", "exceeding", etc. do not include the recited number, and understandings such as "above", "below", "within", etc. include the recited number. If there is a description of "first", "second", etc., it is only for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features or implicitly indicating the sequence relationship of the indicated technical features.

[0084] In the description of the present application, unless otherwise clearly defined, terms such as "set", "installed", "connected", etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present application in combination with the specific content of the technical solution.

[0085] In the description of this specification, the description referring to terms such as "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic descriptions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0086] There is a need to separate products (such as nucleic acids, proteins, etc.) and reaction solutions in biological experiments. In some related technologies, a filtration container is used for separation. A filtration membrane that specifically adsorbs products is provided inside the filtration container. When the reaction solution passes through the filtration membrane, the products will be stably adsorbed on the filtration membrane, while other substances (such as by-products) in the reaction solution will not be adsorbed.

[0087] Specifically, other substances need to be removed first. By adding a cleaning solution to the filtration container for cleaning, other substances can be removed, leaving the purified product on the filtration membrane.

[0088] Next, the product and the filtration membrane need to be separated. By adding an eluent to the filtration container for elution, the eluent can eliminate the adsorption between the product and the filtration membrane, thereby carrying away the product. Collecting the eluent that passes through the filtration membrane can obtain the product, which is convenient for subsequent other experimental operations.

[0089] Generally, pressure is applied to make the liquid (reaction liquid, cleaning solution, eluent, etc.) pass through the filtration container. However, the extraction device in the related art has a relatively single function, usually only capable of applying positive pressure or only capable of applying negative pressure, and it is difficult to meet the different requirements of the impurity cleaning link and the product elution link.

[0090] Therefore, the present application provides an extraction device that can be used to apply both positive pressure and negative pressure, thereby improving the use effect of the extraction device.

[0091] Refer to Figure 1 According to the extraction device provided by the embodiment of the first aspect of the present application, it includes a negative pressure component 1300 and a positive pressure component 1400. The negative pressure component 1300 is used to apply negative pressure to the outlet end of the filtration container 1910, and the positive pressure component 1400 is used to apply positive pressure to the inlet end of the filtration container 1910.

[0092] By configuring the negative pressure component and the positive pressure component, the extraction device can flexibly change the way of applying pressure according to the different requirements of different links in the product extraction process, thereby improving the use effect of the extraction device.

[0093] For example, when cleaning the filtration container 1910, it is often necessary to repeat the process multiple times to ensure the purification effect of the product. At this time, the negative pressure component 1300 can be selected to apply negative pressure to the filtration container 1910. During the application of negative pressure, the positive pressure component 1400 is away from the filtration container 1910, and the inlet end of the filtration container 1910 is always in an open state, so as to facilitate adding the cleaning solution into the filtration container 1910 and improve the operation efficiency of the extraction device.

[0094] When eluting the filtration container 1910, in order to avoid the negative pressure component 1300 sucking away the product, the positive pressure component 1400 can be selected to apply positive pressure to the filtration container 1910. During the application of positive pressure, the negative pressure component 1300 stops generating negative pressure, so as to avoid the product being sucked away by negative pressure and reduce the waste of the product.

[0095] All in all, the extraction device of the present application has a better use effect.

[0096] Optionally, the first tray 1100 can be used to place the filtration container 1910.

[0097] Specifically, the first tray 1100 is formed with a plurality of first placement holes 1110 for placing the filtration container 1910. The filtration containers 1910 can be placed on the first tray 1100 in batches, and the negative pressure assembly 1300 applies negative pressure to the filtration containers 1910 in batches, and the positive pressure assembly 1400 applies positive pressure to the filtration containers 1910 in batches, so as to achieve batch extraction of multiple filtration containers 1910 and further improve the operating efficiency of the extraction device.

[0098] Optionally, the collection container 1920 can be used to receive the product in the elution process, and the second tray 1200 can be used to place the collection container 1920.

[0099] Specifically, the second tray 1200 is used to carry the first tray 1100. The second tray 1200 is formed with a plurality of second placement holes 1210 for placing the collection container 1920, and the collection container 1920 is used to receive the liquid from the filtration container 1910.

[0100] In this way, the product generated during elution will be directly collected by the collection container 1920 and will not come into contact with the negative pressure assembly 1300, which not only avoids contamination of the product by the negative pressure assembly 1300 but also avoids the product remaining on the negative pressure assembly 1300, thereby improving the collection rate of the product.

[0101] It should be noted that the first tray 1100 and the second tray 1200 can be part of the extraction device (that is, the extraction device includes the first tray 1100 and the second tray 1200), or the first tray 1100 and the second tray 1200 can also be consumables sold separately and used in conjunction with the extraction device. This application does not limit this.

[0102] In this embodiment, taking the extraction device including the first tray 1100 and the second tray 1200 as an example, the extraction device will be further introduced.

[0103] At this time, optionally, the negative pressure assembly 1300 can be used to carry either the first tray 1100 or the second tray 1200, and the first tray 1100 and the second tray 1200 can be separated from each other, and the first tray 1100, the second tray 1200 and the negative pressure assembly 1300 can also be separated from each other. When negative pressure needs to be applied, the first tray 1100 can be directly placed on the negative pressure assembly 1300. When positive pressure needs to be applied, the second tray 1200 can be first placed on the negative pressure assembly 1300, and then the first tray 1100 can be placed on the second tray 1200.

[0104] Obviously, this also facilitates the loading and unloading of the filtration container 1910 using the first tray 1100 and the loading and unloading of the collection container 1920 using the second tray 1200. Additionally, the extraction device may include a plurality of first trays 1100 and a plurality of second trays 1200.

[0105] It can be understood that the negative pressure assembly 1300 has a negative pressure hole 1311, and a negative pressure is applied through the negative pressure hole 1311. The negative pressure hole 1311 can be indirectly or directly connected to the outlet end of the filtration container 1910.

[0106] For example, the outlet end of the filtration container 1910 can be received within the first placement hole 1110. When the first tray 1100 is placed on the negative pressure assembly 1300, the negative pressure hole 1311 and the first placement hole 1110 are in communication, and then the outlet end of the filtration container 1910 is connected through the first placement hole 1110.

[0107] At this time, there is a risk of the discharged liquid contaminating the first placement hole 1110, and it may further contaminate the outer surface of the filtration container 1910, ultimately causing cross - contamination between different experiments.

[0108] To this end, optionally, the negative pressure hole 1311 is configured to receive the outlet end of the filtration container 1910.

[0109] That is to say, after the first tray 1100 is placed on the negative pressure assembly 1300, the outlet end of the filtration container 1910 extends into the negative pressure hole 1311, thereby minimizing the process of the liquid from leaving the filtration container 1910 to entering the negative pressure hole 1311 and avoiding contamination of the first placement hole 1110 by the liquid.

[0110] Exemplarily, referring to Figure 2 、 Figure 3 and Figure 4 , the outlet end of the filtration container 1910 can be designed to penetrate through the first tray 1100 to extend into the negative pressure hole 1311.

[0111] Specifically, the first placement hole 1110 penetrates through the first tray 1100, the depth of the first placement hole 1110 is less than the height of the filtration container 1910, and the outlet end of the filtration container 1910 can penetrate through the first tray 1100 to extend into the negative pressure hole 1311.

[0112] At this time, the thickness of the first tray 1100 can be designed with reference to the depth of the first placement hole 1110, so it also helps to reduce the thickness of the first tray 1100 and make the extraction device lighter.

[0113] Of course, the filtration container 1910 can also extend into the negative pressure hole 1311 without penetrating through the first tray 1100.

[0114] Exemplarily, the negative pressure assembly 1300 may have a raised connecting post. The connecting post is hollow to form a negative pressure hole 1311. The connecting post is inserted into the first placement hole 1110, such that the filter container 1910 located within the first placement hole 1110 extends into the negative pressure hole 1311. However, at this time, the thickness of the first tray 1100 is often relatively large (it needs to be greater than the height of the filter container 1910).

[0115] Optionally, referring to Figure 4 , the diameter of the negative pressure hole 1311 is greater than the diameter of the portion of the filter container 1910 inserted into the negative pressure hole 1311, thereby helping to prevent the surfaces of the filter container 1910 and the negative pressure hole 1311 from coming into contact. By reducing the contact between the surfaces of the filter container 1910 and the negative pressure hole 1311, even if the negative pressure hole 1311 is contaminated, it is not easy to re - contaminate the filter container 1910.

[0116] Optionally, referring to Figure 4 , in the vertical direction, the outlet end of the filter container 1910 is aligned with the pressure outlet of the negative pressure hole 1311 (that is, the outlet that communicates with an external air source to generate negative pressure). In this way, the liquid leaving the filter container 1910 can vertically drip under the action of gravity, reach the pressure outlet unobstructed and be discharged from the negative pressure hole 1311, thereby reducing the risk of contamination of other surfaces of the negative pressure hole 1311 (especially the side surface close to the filter container 1910).

[0117] To further reduce the risk of contact between the surfaces of the filter container 1910 and the negative pressure hole 1311, optionally, at least one of the negative pressure assembly 1300 and the first tray 1100 includes a first positioning structure 1312, and the first positioning structure 1312 is used to position the relative positions of the negative pressure assembly 1300 and the first tray 1100.

[0118] The first positioning structure 1312 can make the filter container 1910 and the negative pressure hole 1311 more accurately aligned, avoiding contact caused by assembly errors.

[0119] Exemplarily, referring to Figure 3 and Figure 4 , the negative pressure assembly 1300 is formed with a first receiving groove, and the first tray 1100 can be embedded in the first receiving groove. The side wall of the first receiving groove serves as the first positioning structure 1312.

[0120] Of course, other conventional methods such as positioning pins can also be used to achieve the positioning of the first tray 1100 on the negative pressure assembly 1300. Other feasible positioning structures can refer to relevant existing technologies and will not be elaborated here.

[0121] It can be understood that, referring to Figure 2 and Figure 3, To improve efficiency, cleaning and elution are often carried out in batches. That is, a plurality of first placement holes 1110 can be arranged in an array on the first tray 1100, and a filter container 1910 is installed in each first placement hole 1110. The negative pressure assembly 1300 applies negative pressure to all the filter containers 1910 on the first tray 1100 simultaneously.

[0122] Correspondingly, the base 1300 also needs to be provided with a plurality of negative pressure holes 1311 capable of accommodating the filter containers 1910. For example, Figure 3 as shown in

[0123] the negative pressure holes 1311 are arranged in an array at the bottom of the first receiving groove. If the pressure of each negative pressure hole 1311 is controlled individually, the structure of the negative pressure assembly 1300 will be complicated, which is not conducive to maintenance and use.

[0124] Optionally, the negative pressure assembly 1300 includes a first seat body 1320 and a second seat body 1310. The second seat body 1310 is detachably arranged on the first seat body 1320. The negative pressure holes 1311 are located in the second seat body 1310. The second seat body 1310 and the first seat body 1320 surround and define the first air distribution chamber 1330. By disassembling the first seat body 1320 and the second seat body 1310, the wall surface of the first air distribution chamber 1330 can be exposed, so as to facilitate thorough cleaning of the negative pressure assembly 1300, avoid liquid remaining in the negative pressure holes 1311 and the first air distribution chamber 1330, and cause pollution during the next experiment. In addition, the detachable design is also beneficial to the processing of the first air distribution chamber 1330.

[0125] Optionally, the negative pressure assembly 1300 includes a first sealing member 1340. The first sealing member 1340 is arranged between the first seat body 1320 and the second seat body 1310 to seal the connection part between the first seat body 1320 and the second seat body 1310. The first sealing member 1340 can enhance the sealing effect between the first seat body 1320 and the second seat body 1310, and avoid the occurrence of pressure leakage or liquid overflow.

[0126] The first sealing member 1340 can use a sealing gasket, a sealing ring, a sealant, etc. Exemplarily, referring to Figure 3 and Figure 4, the first seal 1340 is an O-ring. The second seat body 1310 is formed with a seal groove, and the O-ring is placed in the seal groove. The depth of the seal groove is less than the diameter of the O-ring, so that the O-ring is squeezed by the first seat body 1320 to produce a sealing effect.

[0127] In addition, positioning can also be performed between the first seat body 1320 and the second seat body 1310 to ensure the accuracy of the connection relationship.

[0128] Specifically, at least one of the first seat body 1320 and the second seat body 1310 includes a second positioning structure 1321, and the second positioning structure 1321 is used to position the relative positions of the first seat body 1320 and the second seat body 1310.

[0129] Referring to Figure 4 , exemplarily, the first seat body 1320 is formed with a second receiving groove, and the second seat body 1310 can be embedded in the second receiving groove, and the side wall of the second receiving groove serves as the second positioning structure 1321.

[0130] Of course, other conventional methods such as positioning pins can also be used to achieve the positioning of the first seat body 1320 on the second seat body 1310. Other feasible positioning structures can refer to the relevant prior art and will not be elaborated here.

[0131] When positive pressure is applied, the liquid discharged from the filter container 1910 is received by the collection container 1920. Optionally, the collection container 1920 is used to accommodate the outlet end of the filter container 1910.

[0132] That is to say, when the first tray 1100 is placed on the second tray 1200, the outlet end of the filter container 1910 extends into the collection container 1920, thus minimizing the process of the liquid from leaving the filter container 1910 to entering the collection container 1920, ensuring that the product can be fully collected by the collection container 1920 without waste.

[0133] Exemplarily, referring to Figure 5 、 Figure 6 and Figure 7 , the outlet end of the filter container 1910 can be designed to penetrate through the first tray 1100 to extend into the collection container 1920.

[0134] At this time, the thickness of the first tray 1100 can be designed with reference to the depth of the first placement hole 1110, so it also helps to reduce the thickness of the first tray 1100 and make the extraction device lighter.

[0135] Of course, the filter container 1910 may not penetrate through the first tray 1100.

[0136] Exemplarily, when the first tray 1100 is placed on the second tray 1200, the collection container 1920 extends into the first placement hole 1110, so that the outlet end of the filter container 1910 extends into the collection container 1920. However, at this time, the thickness of the first tray 1100 is often relatively large (it needs to be greater than the height of the filter container 1910).

[0137] It can be understood that positioning is also required between the first tray 1100 and the second tray 1200, so that the first placement hole 1110 and the second placement hole 1210 can be accurately aligned, and the filter container 1910 and the collection container 1920 can be accurately docked.

[0138] Optionally, at least one of the second tray 1200 and the first tray 1100 includes a third positioning structure 1220, and the third positioning structure 1220 is used to position the relative positions of the second tray 1200 and the first tray 1100.

[0139] Exemplarily, the second tray 1200 is formed with a third receiving groove, and the first tray 1100 can be embedded in the third receiving groove, and the side wall of the third receiving groove serves as the third positioning structure 1220.

[0140] It should be noted that, in order to simplify the design of the first tray 1100, the first positioning structure 1312 and the third positioning structure 1220 can be designed to contact and position the same part of the first tray 1100. For example, referring to Figure 3 and Figure 6 , the first receiving groove and the third receiving groove adopt the same shape design, and both the first positioning structure 1312 and the third positioning structure 1220 achieve positioning by contacting the side surface of the first tray 1100.

[0141] Of course, other conventional methods such as positioning pins can also be used to achieve the positioning of the first tray 1100 on the second tray 1200. Other feasible positioning structures can refer to the relevant prior art and will not be elaborated here.

[0142] Optionally, referring to Figure 7 , at the bottom of the first tray 1100, the diameter of the second placement hole 1210 is larger than the diameter of the collection container 1920 to avoid the collection container 1920. That is to say, when the first tray 1100 is placed on the second tray 1200, the first tray 1100 does not press on the collection container 1920, and the second tray 1200 contacts the first tray 1100 and bears the force of the first tray 1100 to avoid damaging the collection container 1920.

[0143] To reduce the thickness of the second tray 1200 and lighten its weight, optionally, the second placement hole 1210 penetrates through the second tray 1200. The depth of the second placement hole 1210 is less than the height of the collection container 1920, and the collection container 1920 penetrates through the second tray 1200.

[0144] Furthermore, the negative pressure hole 1311 can be used to accommodate the collection container 1920 that penetrates through the second tray 1200. That is to say, the negative pressure hole 1311 plays a role of avoidance and accommodation when positive pressure is applied. By enriching the function of the negative pressure hole 1311, it is possible to avoid additionally setting other structures on the negative pressure component 1300 to receive the second tray 1200 with the collection container 1920 placed thereon, which helps to simplify the structural design of the extraction device.

[0145] Optionally, the diameter of the negative pressure hole 1311 is larger than the diameter of the part of the collection container 1920 inserted into the negative pressure hole 1311, so as to prevent the wall surface of the negative pressure hole 1311 from contacting the surface of the collection container 1920. Since there is a certain distance between the wall surface of the negative pressure hole 1311 and the surface of the collection container 1920, even if the wall surface of the negative pressure hole 1311 is accidentally contaminated, the contaminants are not likely to contaminate the collection container 1920 again.

[0146] Taking advantage of the distance left between the wall surface of the negative pressure hole 1311 and the surface of the collection container 1920, when the collection container 1920 is placed in the negative pressure hole 1311, the negative pressure hole 1311 can also be used for negative pressure elution of the filtration container 1910, and the collection container 1920 is used to collect the elution product (eluent).

[0147] Specifically, please refer to Figure 8 and Figure 9 , there is a first gap 1810 between the outer wall of the filtration container 1910 and the inner wall of the collection container 1920, a second gap 1820 between the outer wall of the collection container 1920 and the inner wall of the second placement hole 1210, and a third gap 1830 between the outer wall of the collection container 1920 and the inner wall of the negative pressure hole 1311. The filtration container 1910, the collection container 1920, the first gap 1810, the second gap 1820, and the third gap 1830 are sequentially connected to form a negative pressure elution gas path. As shown in Figure 9 , the dotted line path in the figure represents the negative pressure elution gas path. Through the settings of the first gap 1810, the second gap 1820, and the third gap 1830, an air flow path can be formed between the filtration container 1910 and the negative pressure hole 1311. When an external air source evacuates the negative pressure hole 1311, the vacuum effect can act on the filtration container 1910 through the negative pressure elution gas path to realize the product elution operation in the filtration container 1910, and the eluent can be collected by the collection container 1920, avoiding the waste caused by the air source sucking away the eluent.

[0148] Optionally, there is a fourth gap 1840 between the outer wall of the collection container 1920 and the inner wall of the first placement hole 1110, and the fourth gap 1840 communicates with the negative pressure elution gas path. In order to enable the collection container 1920 to be held in the second placement hole 1210 of the second tray 1200, a flanging structure 1921 is provided on the outer periphery of the opening of the collection container 1920. When the collection container 1920 is placed in the second placement hole 1210, the flanging structure 1921 is located at the outer edge of the second placement hole 1210 and protrudes from the surface of the second placement hole 1210. When the first tray 1100 is placed on the surface of the second tray 1200, a stepped hole 1111 for avoiding the flanging structure 1921 is provided in the first placement hole 1110, and a fourth gap 1840 is formed between the inner wall of the stepped hole 1111 and the flanging structure 1921 of the collection container 1920. In this way, the fourth gap 1840 can communicate with the first gap 1810 and the second gap 1820 respectively, so as to form a negative pressure elution gas path. The vacuum pumping effect of the external air source on the negative pressure hole 1311 can act on the collection container 1920 and the filtration container 1910 through the third gap 1830, the second gap 1820, the fourth gap 1840, and the first gap 1810 in turn, realizing the negative pressure elution effect.

[0149] It can be understood that in order to accurately place the second tray 1200 on the negative pressure assembly 1300, the second tray 1200 also needs to be positioned. Optionally, the first positioning structure 1312 can also be used to position the relative positions of the negative pressure assembly 1300 and the second tray 1200.

[0150] It can be understood that the positive pressure assembly 1400 has a positive pressure hole 1411, and the positive pressure hole 1411 communicates with the inlet end of the filtration container 1910, so as to apply positive pressure to the filtration container 1910. When applying positive pressure, the positive pressure assembly 1400 needs to accurately cover the filtration container 1910 to prevent pressure leakage.

[0151] Optionally, please refer to Figure 10 , the positive pressure hole 1411, the filtration container 1910, and the collection container 1920 are sequentially communicated to form a positive pressure elution gas path. Figure 10 The dotted line path in represents the positive pressure elution gas path. The positive pressure assembly 1400 can apply air pressure to the filtration container 1910 by using the positive pressure elution gas path, and the eluted product can enter the collection container 1920 under positive pressure, thus completing elution and collection.

[0152] Optionally, the negative pressure elution gas path and the positive pressure elution gas path may partially overlap. Specifically, the negative pressure elution gas path and the positive pressure elution gas path are arranged to coincide in the filter container 1910 and the collection container 1920. In this way, positive pressure elution, negative pressure elution, or both positive pressure elution and negative pressure elution can be implemented in the extraction device. On the one hand, the same extraction device can be used to perform positive pressure elution or negative pressure elution respectively, which can improve the versatility and practicality of the extraction device. During the elution stage, positive pressure or negative pressure elution can be flexibly selected according to actual needs, and the solution does not need to be transferred to other devices, thus improving the convenience of operating the extraction device. On the other hand, when a relatively large elution pressure needs to be applied to the liquid, by simultaneously applying positive pressure and negative pressure, a greater pressure can be obtained, which helps to accelerate the elution process and improve the operation efficiency.

[0153] Optionally, at least one of the positive pressure assembly 1400 and the first tray 1100 includes a fourth positioning structure 1412, and the fourth positioning structure 1412 is used to position the relative positions of the positive pressure assembly 1400 and the first tray 1100.

[0154] Exemplarily, referring to Figure 7 , the positive pressure holes 1411 are located on the bottom surface of the positive pressure assembly 1400, and the bottom surface of the positive pressure assembly 1400 presses against the top surface of the first tray 1100, so that the positive pressure holes 1411 communicate with the filter container 1910.

[0155] At this time, the positive pressure assembly 1400 may be formed with a fourth receiving groove, and the first tray 1100 can be embedded in the fourth receiving groove, and the side wall of the fourth receiving groove serves as the fourth positioning structure 1412.

[0156] Of course, other conventional methods such as positioning pins can also be used to achieve the positioning of the positive pressure assembly 1400 on the first tray 1100. Other feasible positioning structures can refer to the relevant existing technologies and will not be elaborated here.

[0157] Optionally, the extraction device may further include a second seal 1510, and the second seal 1510 is arranged between the positive pressure assembly 1400 and the first tray 1100, and the second seal 1510 is used to seal the connection part between the positive pressure assembly 1400 and the first tray 1100. Specifically, referring to Figure 7 , an annular seal groove can be formed on the outer periphery of each positive pressure hole 1411, and the second seal 1510 is arranged in the seal groove, so as to provide a corresponding second seal 1510 for each positive pressure hole 1411. Alternatively, a seal groove surrounding all the positive pressure holes 1411 can also be formed, and multiple positive pressure holes 1411 share the same second seal 1510, as long as it is ensured that the pressure does not leak.

[0158] It is understandable that the positive pressure component 1400 also needs to be provided with a plurality of positive pressure holes 1411 that can communicate with the filter container 1910. For example, Figure 6 , Figure 7 the bottom surface of the positive pressure component 1400 shown in is provided with positive pressure holes 1411 in an array. If the pressure of each positive pressure hole 1411 is controlled individually, the structure of the positive pressure component 1400 will be complicated, which is not conducive to maintenance and use.

[0159] To simplify the design of the positive pressure component 1400, optionally, the positive pressure component 1400 is formed with a second air distribution chamber 1430. Each positive pressure hole 1411 communicates with the second air distribution chamber 1430 respectively, and the second air distribution chamber 1430 is used to communicate with an external air source.

[0160] By relaying through the second air distribution chamber 1430, multiple positive pressure holes 1411 can be controlled at one time, thereby simplifying the air flow distribution method.

[0161] Optionally, the positive pressure component 1400 includes a first cover 1420 and a second cover 1410. The second cover 1410 is detachably arranged on the first cover 1420. The positive pressure holes 1411 are located on the second cover 1410, and the second cover 1410 and the first cover 1420 surround and define the second air distribution chamber 1430. The split design is conducive to the processing of the second air distribution chamber 1430.

[0162] Optionally, the positive pressure component 1400 includes a third seal 1440. The third seal 1440 is arranged between the first cover 1420 and the second cover 1410 to seal the connection part between the first cover 1420 and the second cover 1410. The third seal 1440 can enhance the sealing effect between the first cover 1420 and the second cover 1410 and avoid pressure leakage.

[0163] The third seal 1440 can use a sealing gasket, a sealing ring, a sealant, etc. Exemplarily, referring to Figure 7 , the third seal 1440 is a sealing ring. The second cover 1410 is formed with a sealing groove, and the sealing ring is placed in the sealing groove. The depth of the sealing groove is less than the diameter of the sealing ring, so that the sealing ring is extruded by the first cover 1420 to generate a sealing effect.

[0164] In addition, the first cover 1420 and the second cover 1410 can also be positioned to ensure the accuracy of the connection relationship.

[0165] Specifically, at least one of the first cover 1420 and the second cover 1410 includes a fifth positioning structure 1421, and the fifth positioning structure 1421 is used to position the relative positions of the first cover 1420 and the second cover 1410.

[0166] Referring toFigure 7 Exemplarily, a fifth receiving groove is formed in the first cover body 1420, and the second cover body 1410 can be embedded in the fifth receiving groove. The side wall of the fifth receiving groove serves as the fifth positioning structure 1421.

[0167] Of course, other conventional methods such as positioning pins can also be used to achieve the positioning of the first cover body 1420 on the second cover body 1410. Other feasible positioning structures can refer to the relevant existing technologies and will not be elaborated here.

[0168] During the actual experiments related to extracting products, this application noticed that due to the unsatisfactory effect of a single cleaning, impurities may still remain in the filtration container 1910, and multiple cleanings are often required, that is, the operations of adding cleaning liquid and applying pressure are alternately performed.

[0169] However, when cleaning is performed by applying positive pressure, the positive pressure assembly 1400 needs to cover the inlet end of the filtration container 1910, that is, to isolate the inlet end of the filtration container 1910 from the external environment, so that positive pressure can be applied through the positive pressure hole 1411. When adding cleaning liquid, the positive pressure assembly 1400 needs to be removed to expose the inlet end, resulting in a relatively cumbersome operation process.

[0170] In addition, when eluting by applying negative pressure, the eluent may be directly drained through the negative pressure hole 1311 under the action of negative pressure, resulting in insufficient collection of the product.

[0171] Therefore, this application also provides a product extraction method, which can at least simplify the operation process and improve the collection rate of the product.

[0172] In one embodiment, according to the product extraction method provided by the embodiment of this application, it includes:

[0173] Applying negative pressure to the filtration container 1910 containing cleaning liquid for cleaning;

[0174] Applying positive pressure to the filtration container 1910 containing eluent for elution.

[0175] Since negative pressure is applied during cleaning, the inlet end of the filtration container 1910 can be kept open at this time, facilitating the addition of cleaning liquid to the filtration container 1910 for multiple rounds of cleaning; since positive pressure is applied during elution, the outlet end of the filtration container 1910 does not need to be connected to a negative pressure channel (such as the negative pressure hole 1311) at this time, facilitating the collection of eluent at the outlet end. Therefore, the product extraction method is at least used to simplify the operation process and improve the collection rate of the product.

[0176] The product extraction method can be implemented by the extraction device of this application.

[0177] Taking the extraction device according to the embodiment of the first aspect of the present application as an example, applying negative pressure to the filtration container 1910 filled with the cleaning liquid may include:

[0178] Place the filtration container 1910 into the first tray 1100;

[0179] Place the first tray 1100 on the negative pressure assembly 1300;

[0180] Add the cleaning liquid to the filtration container 1910;

[0181] Apply negative pressure to the outlet end of the filtration container 1910 through the negative pressure holes 1311 of the first tray 1100.

[0182] At this time, the positive pressure assembly 1400 is away from the negative pressure assembly 1300, and the inlet end of the filtration container 1910 is open, facilitating the addition of the cleaning liquid.

[0183] Optionally, the operations of adding the cleaning liquid and applying negative pressure can be alternately performed to improve the cleaning effect.

[0184] Applying positive pressure to the filtration container 1910 filled with the eluent may include:

[0185] Place the collection container 1920 into the second tray 1200;

[0186] Place the second tray 1200 on the negative pressure assembly 1300;

[0187] Place the first tray 1100 on the second tray 1200;

[0188] Add the eluent to the filtration container 1910;

[0189] The positive pressure assembly 1400 presses on the first tray 1100;

[0190] Apply positive pressure to the inlet end of the filtration container 1910 through the positive pressure holes 1411 of the positive pressure assembly 1400.

[0191] At this time, the outlet end of the filtration container 1910 is docked with the collection container 1920, and the eluent leaving the filtration container 1910 is collected by the collection container 1920, facilitating the improvement of the product collection rate.

[0192] Please refer to Figure 8 and Figure 9 , in another embodiment, according to the product extraction method provided by the embodiment of the present application, it includes:

[0193] Apply negative pressure to the filtration container 1910 filled with the cleaning liquid for cleaning;

[0194] Place the filtration container 1910 on the collection container 1920, and apply negative pressure to the filtration container 1910 filled with the eluent for elution.

[0195] By applying negative pressure during cleaning, the inlet end of the filter container 1910 can be kept open, facilitating the addition of cleaning liquid into the filter container 1910 for multiple rounds of cleaning. By placing the filter container 1910 in the collection container 1920, when performing negative pressure elution on the filter container 1910, the collection container 1920 can be used to collect the eluate, preventing the eluate product from being drawn away by the pressure source, improving the collection rate of the product, and thus enhancing the experimental efficiency.

[0196] Taking the extraction device according to the embodiment of the first aspect of the present application as an example, when the filter container 1910 is placed in the collection container 1920 and negative pressure is applied to the filter container 1910 containing the eluate, it may include:

[0197] Put the filter container 1910 into the first tray 1100;

[0198] Put the collection container 1920 into the second tray 1200;

[0199] Place the second tray 1200 on the negative pressure assembly 1300;

[0200] Place the first tray 1100 on the second tray 1200;

[0201] Add eluate to the filter container 1910;

[0202] The negative pressure hole 1311 of the first tray 1100 applies negative pressure to the outlet end of the filter container 1910.

[0203] In this way, by utilizing the mutual cooperation of the first tray 1100 and the second tray 1200,

[0204] It can be understood that a negative pressure passage can be formed between the filter container 1910 and the negative pressure hole 1311. As Figure 9 shown, the dashed arrow shows the gas path diagram when the external gas source applies negative pressure to the filter container 1910. The vacuuming effect of the external gas source can act on the filter container 1910 through the collection container 1920, enabling the reaction product to be eluted in the filter container 1920 and collected by the collection container 1920. That is, the collection container 1920 does not affect the negative pressure effect of the external gas source on the filter container 1910.

[0205] Optionally, please refer to Figure 10 , while performing negative pressure elution on the filter container 1910, positive pressure can also be applied to the filter container 1910 simultaneously to improve the elution efficiency. Specifically, after adding eluate to the filter container 1910, the product extraction method includes:

[0206] The positive pressure assembly 1400 presses on the first tray 1100;

[0207] The positive pressure hole 1411 of the positive pressure assembly 1400 applies positive pressure to the inlet end of the filter container 1910 .

[0208] Figure 10 The dashed arrow in the middle shows the air path when the external air source applies negative pressure to the filter container 1910, and the dotted arrow shows the air path when the external air source applies positive pressure to the filter container 1910. In this way, positive pressure is applied to the filter container 1910 using the positive pressure component 1400, and negative pressure is applied to the filter container 1910 using the negative pressure component 1300. Under the superposition of positive pressure and negative pressure, the filter container 1910 can obtain a greater pressure difference with the reference air pressure (such as atmospheric pressure), thereby achieving a better elution effect. Compared with the case of applying only positive pressure or only negative pressure, applying positive pressure and negative pressure at the same time can shorten the elution time of the filter container 1910 and improve the elution efficiency. Or in other experimental scenarios, for example, in the situation where a large pressure difference is required to achieve elution, the simultaneous application of positive pressure and negative pressure in this embodiment can reduce the power requirements for the positive pressure air source or the negative pressure air source.

[0209] It can be understood that the extraction device of the present application can be operated manually or automatically. In order to facilitate the automation of the extraction process, the present application further provides an extraction platform including the extraction device, and the extraction platform is suitable for automated extraction.

[0210] Reference Figure 11 In addition to the extraction device, the extraction platform also includes a movable seat 1610 and a first driving member 1620. The negative pressure assembly 1300 is arranged on the movable seat 1610. The first driving member 1620 can drive the movable seat 1610 to move horizontally so that the negative pressure assembly 1300 is aligned or staggered with the positive pressure assembly 1400 in the vertical direction.

[0211] When loading and unloading or other operations are required, the negative pressure assembly 1300 and the positive pressure assembly 1400 are staggered, so that the negative pressure assembly 1300 is exposed to the outside, which is convenient for placing the first tray 1100 and the second tray 1200 and performing related operations. When pressure needs to be applied, the negative pressure assembly 1300 and the positive pressure assembly 1400 are aligned, and the positive pressure assembly 1400 is pressed toward the negative pressure assembly 1300, thereby pressing the first tray 1200 and the second tray 1300 between the two to ensure the stability of the connection relationship so as to reliably apply pressure.

[0212] The movable seat 1610 can drive the negative pressure assembly 1300 to move by moving, rotating, etc. Figure 11 In the embodiment, the movable seat 1610 is arranged on the screw rod, and the first driving member 1620 is a rotating motor. The first driving member 1620 drives the screw rod to rotate, so that the movable seat 1610 moves along the screw rod.

[0213] In addition, in order to determine the location of the negative pressure assembly 1300, the extraction platform may further include a position sensor 1630 for detecting the position of the negative pressure assembly 1300. The position sensor 1630 may use sensor types commonly used in related technologies such as photoelectric sensors, grating scales, distance sensors, etc., which will not be elaborated here.

[0214] Optionally, the extraction platform further includes a second driving member 1710 for driving the positive pressure assembly 1400 to approach or move away from the negative pressure assembly 1300.

[0215] Optionally, the extraction platform further includes a guide member 1720 extending in the vertical direction, and the positive pressure assembly 1400 is connected to the guide member 1720.

[0216] It should be noted that the extraction platform may include multiple extraction devices to facilitate the design of parallel extraction steps, that is, when a part of the extraction devices perform the elution operation of the current batch of filtration containers 1910, another part of the extraction devices perform the cleaning operation of the next batch of filtration containers 1910, thereby improving the extraction efficiency.

[0217] In some alternative embodiments, the functions / operations mentioned in the block diagrams may not occur in the order mentioned in the operation diagrams. For example, depending on the functions / operations involved, two consecutively shown blocks may actually be executed substantially simultaneously or the blocks can sometimes be executed in the reverse order. In addition, the embodiments presented and described in the flowcharts of the present application are provided by way of example for the purpose of providing a more comprehensive understanding of the technology. The disclosed methods are not limited to the operations and logical flows presented herein. Alternative embodiments are contemplated where the order of various operations is changed and where sub-operations described as part of a larger operation are executed independently.

[0218] Although the embodiments of the present application have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present application. The scope of the present application is defined by the claims and their equivalents.

Claims

1. An extraction device, characterized in that: include: A negative pressure assembly, used to apply negative pressure to the outlet end of the filter container; The positive pressure assembly is used to apply positive pressure to the inlet end of the filter container.

2. The extraction device according to claim 1, characterized in that The extraction device comprises a first tray, wherein the first tray is formed with a plurality of first placement holes, and the first placement holes are used for placing the filter container.

3. The extraction device according to claim 2, characterized in that The negative pressure assembly is formed with a negative pressure hole, and the negative pressure hole is used to receive the outlet end of the filter container.

4. The extraction device according to claim 3, characterized in that The first placement hole passes through the first tray, the depth of the first placement hole is less than the height of the filter container, and the outlet end of the filter container passes through the first tray to extend into the negative pressure hole.

5. The extraction device according to claim 4, characterized in that: The negative pressure hole has a diameter greater than a diameter of a portion of the filter container inserted into the negative pressure hole.

6. The extraction device according to claim 2, characterized in that: At least one of the negative pressure assembly and the first tray comprises a first positioning structure, and the first positioning structure is used to locate the relative positions of the negative pressure assembly and the first tray.

7. The extraction device according to claim 6, characterized in that The negative pressure assembly is formed with a first receiving groove, the first tray can be embedded in the first receiving groove, and the side wall of the first receiving groove serves as the first positioning structure.

8. The extraction device according to claim 3, characterized in that: The negative pressure assembly is formed with a first air distribution chamber, and each of the negative pressure holes is communicated with the first air distribution chamber respectively, and the first air distribution chamber is used to connect to an external air source.

9. The extraction device according to claim 8, characterized in that The negative pressure assembly includes a first seat body and a second seat body, the second seat body is detachably arranged on the first seat body, the negative pressure hole is located in the second seat body, and the second seat body and the first seat body surround and define the first air distribution chamber.

10. The extraction device according to claim 9, characterized in that At least one of the first base and the second base includes a second positioning structure, and the second positioning structure is used to locate the relative positions of the first base and the second base.

11. The extraction device according to claim 10, characterized in that The first seat body is formed with a second receiving groove, the second seat body can be embedded in the second receiving groove, and the side wall of the second receiving groove serves as the second positioning structure.

12. The extraction device according to claim 9, characterized in that The negative pressure assembly includes a first seal, which is disposed between the first seat body and the second seat body to seal a connection portion between the first seat body and the second seat body.

13. The extraction device according to claim 2, characterized in that: The extraction device comprises a second tray, the second tray is used to carry the first tray, the second tray is formed with a plurality of second placement holes, the second placement holes are used to place a collection container, and the collection container is used to receive the liquid from the filtering container.

14. The extraction device according to claim 13, characterized in that The first placement hole passes through the first tray, the depth of the first placement hole is smaller than the height of the filter container, and the outlet end of the filter container passes through the first tray to extend into the collection container.

15. The extraction device according to claim 13, characterized in that At least one of the second tray and the first tray includes a third positioning structure, and the third positioning structure is used to locate the relative position of the second tray and the first tray.

16. The extraction device according to claim 15, characterized in that The second tray is formed with a third receiving groove, the first tray can be embedded in the third receiving groove, and the side wall of the third receiving groove serves as the third positioning structure.

17. The extraction device according to claim 16, characterized in that At the bottom of the first tray, the diameter of the second placement hole is larger than the diameter of the collection container so as to avoid the collection container.

18. The extraction device according to claim 13, characterized in that The second placement hole passes through the second tray, the depth of the second placement hole is less than the height of the collection container, and the collection container passes through the second tray.

19. The extraction device according to claim 18, characterized in that The negative pressure assembly is formed with a negative pressure hole, which is used to accommodate the collection container passing through the second tray, and the diameter of the negative pressure hole is larger than the diameter of the portion of the collection container inserted into the negative pressure hole.

20. The extraction device according to claim 19, characterized in that There is a first gap between the outer wall of the filtration container and the inner wall of the collection container, a second gap between the outer wall of the collection container and the inner wall of the second placement hole, and a third gap between the outer wall of the collection container and the inner wall of the negative pressure hole. The filtration container, the collection container, the first gap, the second gap and the third gap are connected in sequence to form a negative pressure elution gas path.

21. The extraction device according to claim 20, characterized in that A fourth gap is provided between the outer wall of the collecting container and the inner wall of the first placement hole, and the fourth gap is connected to the negative pressure elution gas path.

22. The extraction device according to claim 13, characterized in that The negative pressure assembly includes a first positioning structure, and the first positioning structure is used to locate the relative positions of the negative pressure assembly and the second tray.

23. The extraction device according to claim 2, characterized in that The positive pressure assembly is formed with a positive pressure hole, and the positive pressure hole is used to communicate with the inlet end of the filter container.

24. The extraction device according to claim 23, characterized in that The positive pressure hole, the filter container and the collection container are connected in sequence to form a positive pressure elution gas path.

25. The extraction device according to claim 24, characterized in that The extraction device further comprises a negative pressure elution gas path, and the negative pressure elution gas path and the positive pressure elution gas path are arranged to overlap in the filtering container and the collecting container.

26. The extraction device according to claim 23, characterized in that At least one of the positive pressure assembly and the first tray includes a fourth positioning structure, and the fourth positioning structure is used to locate the relative positions of the positive pressure assembly and the first tray.

27. The extraction device according to claim 26, characterized in that The positive pressure assembly is formed with a fourth receiving groove, the first tray can be embedded in the fourth receiving groove, and the side wall of the fourth receiving groove serves as the fourth positioning structure.

28. The extraction device according to claim 27, characterized in that The extraction device includes a second sealing member, which is disposed between the positive pressure component and the first tray, and is used to seal a connection portion between the positive pressure component and the first tray.

29. The extraction device according to claim 28, characterized in that An annular sealing groove is formed on the outer periphery of each of the positive pressure holes, and the second sealing member is arranged in the sealing groove.

30. The extraction device according to claim 23, characterized in that The positive pressure assembly is formed with a second air distribution chamber, and each of the positive pressure holes is communicated with the second air distribution chamber respectively, and the second air distribution chamber is used to connect to an external air source.

31. The extraction device according to claim 30, characterized in that The positive pressure assembly includes a first cover body and a second cover body, the second cover body is detachably arranged on the first cover body, the positive pressure hole is located in the second cover body, and the second cover body and the first cover body surround and define the second air distribution chamber.

32. The extraction device according to claim 31, characterized in that At least one of the first cover body and the second cover body includes a fifth positioning structure, and the fifth positioning structure is used to locate the relative positions of the first cover body and the second cover body.

33. The extraction device according to claim 32, characterized in that The first cover body is formed with a fifth receiving groove, the second cover body can be embedded in the fifth receiving groove, and the side wall of the fifth receiving groove serves as the fifth positioning structure.

34. The extraction device according to claim 31, characterized in that The positive pressure assembly includes a third seal, which is disposed between the first cover body and the second cover body to seal a connection portion between the first cover body and the second cover body.

35. An extraction platform, characterized in that: The extraction platform comprises the extraction device according to any one of claims 1 to 34.

36. The extraction platform according to claim 35, characterized in that The extraction platform also includes a movable seat and a first driving member, the negative pressure component is arranged on the movable seat, and the first driving member can drive the movable seat to move horizontally so that the negative pressure component is aligned or staggered with the positive pressure component in the vertical direction.

37. The extraction platform according to claim 36, characterized in that The extraction platform further includes a position sensor, and the position sensor is used to detect the position of the negative pressure component.

38. The extraction platform according to claim 36, characterized in that The extraction platform further includes a second driving member, which is used to drive the positive pressure component to approach or move away from the negative pressure component.

39. The extraction platform according to claim 38, characterized in that The extraction platform further comprises a guide member extending in a vertical direction, and the positive pressure assembly is connected to the guide member.

40. A product extraction method, characterized in that: include: Applying negative pressure to the filter container into which the cleaning liquid is added to perform cleaning; Positive pressure is applied to the filtration container into which the eluent is added to perform elution.

41. The product extraction method according to claim 40, characterized in that Apply negative pressure to the filter container with the cleaning solution added, including: The filter container is placed in a first tray; The first tray is placed in the negative pressure assembly; Adding the cleaning liquid into the filtering container; The negative pressure hole of the first tray applies negative pressure to the outlet end of the filter container.

42. The product extraction method according to claim 41, characterized in that Applying positive pressure to the filtration container into which the eluent is added comprises: The collection container is placed in the second tray; The second tray is placed on the negative pressure assembly; The first tray is placed on the second tray; Adding the eluent into the filtration container; The positive pressure assembly presses the first tray; The positive pressure hole of the positive pressure assembly applies positive pressure to the inlet end of the filter container.

43. A product extraction method, characterized in that: include: Applying negative pressure to the filter container into which the cleaning liquid is added to perform cleaning; The filter container is placed in a collection container, and negative pressure is applied to the filter container into which the eluent is added to perform elution.

44. The product extraction method according to claim 43, characterized in that The filtering container is placed in the collecting container, and negative pressure is applied to the filtering container to which the eluent is added, comprising: The filter container is placed in a first tray; The collection container is placed in the second tray; The second tray is placed on the negative pressure assembly; The first tray is placed on the second tray; Adding the eluent into the filtration container; The negative pressure hole of the first tray applies negative pressure to the outlet end of the filter container.

45. The product extraction method according to claim 44, characterized in that After the eluent is added to the filtration container, the product extraction method comprises: The positive pressure assembly presses the first tray; The positive pressure hole of the positive pressure assembly applies positive pressure to the inlet end of the filter container.

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