Application of soluble organic carbon fertilizer to flue-cured tobacco

Abstract

The invention discloses application of a soluble organic carbon fertilizer to flue-cured tobaccos, which is characterized in that the soluble organic carbon fertilizer is applied to promote the growth and development of the flue-cured tobaccos, improve the physiological and biochemical characteristics of the flue-cured tobaccos and improve the quality of the flue-cured tobaccos, and the application amount of the soluble organic carbon fertilizer is 300kg / hm < 2 > to 900kg / hm < 2 >; by applying the soluble organic carbon fertilizer, growth and development of the flue-cured tobacco can be promoted, stress resistance and carbon and nitrogen metabolism can be enhanced, specifically, agronomic traits and root morphology are improved, root activity is enhanced, photosynthesis is improved, the tissue structure is improved, activity of antioxidant enzymes and carbon and nitrogen metabolism enzymes is improved, the content of malondialdehyde and H2O2 is reduced, and meanwhile, the content of the flue-cured tobacco can be increased. The quality of the flue-cured tobacco can also be improved by applying the soluble organic carbon fertilizer, specifically, the appearance quality is improved, the sensory quality and chemical components are more coordinated, the organic acid content is increased, the effect of applying the soluble organic carbon fertilizer with the concentration of 600kg / hm < 2 > is optimal, and a basis can be provided for application and popularization of the organic carbon fertilizer on the tobacco.

Description

Technical Field

[0001] The invention relates to the technical field of organic carbon fertilizer application, and in particular to the application of soluble organic carbon fertilizer to flue-cured tobacco. Background Art

[0002] As an important cash crop, tobacco occupies an important position in my country's agricultural production and has a significant impact on national tax revenue and farmers' income. However, long-term reliance on traditional fertilizer application methods has led to a series of problems such as decreased soil fertility, soil compaction, and reduced organic matter content. These problems not only limit the growth and development of flue-cured tobacco, but also affect the quality of tobacco leaves. Therefore, how to solve these problems by improving fertilizer application methods and selecting new fertilizers has become a research focus in the field of tobacco cultivation.

[0003] In recent years, the use of organic fertilizers has received more and more attention, especially soluble organic carbon fertilizers that can quickly dissolve and effectively supplement nutrients. As a new type of fertilizer, soluble organic carbon fertilizer is water-soluble and fast-acting. It is rich in organic carbon and other effective nutrients. Its effective carbon content is about 20 times that of ordinary organic fertilizers. It can effectively increase the soil organic matter content, improve soil structure, enhance crop resistance and promote crop growth in a short time.

[0004] In the research of other crops, the application of soluble organic carbon fertilizer has shown obvious effects. For example, studies have shown that the use of organic carbon fertilizer can improve the root development of plants, improve the efficiency of photosynthesis, enhance antioxidant capacity, and promote carbon and nitrogen metabolism, thereby improving the quality of crops. However, although organic carbon fertilizer has achieved good application results in other crops, the research on flue-cured tobacco is still relatively limited, especially its effects on the growth, stress resistance and quality improvement of flue-cured tobacco have not been systematically verified. To solve the above problems, we proposed an application of soluble organic carbon fertilizer on flue-cured tobacco. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides an application of a soluble organic carbon fertilizer to flue-cured tobacco. By conducting field tests on soluble organic carbon fertilizers with different application amounts, the effects on the growth, root development, photosynthesis, antioxidant capacity, carbon-nitrogen metabolic enzyme activity and quality of flue-cured tobacco are explored. The purpose is to provide a scientific basis for the application of soluble organic carbon fertilizers in flue-cured tobacco production, thereby promoting the efficient and healthy growth of flue-cured tobacco and improving its yield and quality, thereby solving the problems raised in the background technology.

[0006] The above technical objectives of the present invention are achieved through the following technical solutions:

[0007] The invention discloses an application of soluble organic carbon fertilizer to flue-cured tobacco, characterized in that the application of the soluble organic carbon fertilizer promotes the growth and development of flue-cured tobacco, improves the physiological and biochemical characteristics of flue-cured tobacco and improves the quality of flue-cured tobacco.

[0008] Furthermore, the application amount of the soluble organic carbon fertilizer is 300 kg / hm 2 Up to 900kg / hm 2 .

[0009] Furthermore, the soluble organic carbon fertilizer is a water-soluble fertilizer rich in effective carbon, and its effective carbon content is 20 times that of ordinary organic fertilizer.

[0010] Furthermore, the application of the soluble organic carbon fertilizer can promote the growth of the root system of flue-cured tobacco, increase the total root length, root surface area, root volume, the number of root tips, and improve the root activity.

[0011] Furthermore, the application of the soluble organic carbon fertilizer can improve the photosynthesis efficiency of flue-cured tobacco, increase the net photosynthetic rate, stomatal conductance and transpiration rate, and reduce the carbon dioxide concentration.

[0012] Furthermore, the application of the soluble organic carbon fertilizer can enhance the antioxidant capacity of flue-cured tobacco, increase the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and reduce malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 )content.

[0013] Furthermore, the application of the soluble organic carbon fertilizer can promote the activity of carbon-nitrogen metabolic enzymes in flue-cured tobacco, increase the activity of sucrose synthase (SS) and sucrose phosphate synthase (SPS), and optimize carbon-nitrogen metabolism.

[0014] Furthermore, the application of the soluble organic carbon fertilizer can improve the leaf tissue structure of flue-cured tobacco, which is manifested in the increase of leaf flesh thickness, palisade tissue thickness, and spongy tissue thickness.

[0015] Furthermore, the application of the soluble organic carbon fertilizer can improve the appearance quality, sensory quality and chemical composition of the flue-cured tobacco leaves, especially increase the organic acid content, which is manifested by the increase in the content of organic acids such as malonic acid, malic acid, and citric acid.

[0016] Furthermore, the application of the soluble organic carbon fertilizer can increase the sugar content, reducing sugar, and total sugar content of flue-cured tobacco, optimize the potassium-chlorine ratio and the sugar-alkali ratio, and improve the chemical composition and quality of tobacco leaves. The application of the soluble organic carbon fertilizer can increase the potassium and starch content of flue-cured tobacco, and make the potassium-chlorine ratio of flue-cured tobacco reach the standard of high-quality tobacco.

[0017] In summary, the present invention mainly has the following beneficial effects:

[0018] 1. The present invention promotes the growth and development of flue-cured tobacco by applying soluble organic carbon fertilizer, improves its root growth, significantly increases the total root length, root surface area, root volume and root tip number, thereby improving root activity and enhancing the nutrient absorption capacity of flue-cured tobacco. In addition, the application of soluble organic carbon fertilizer can also improve the photosynthesis efficiency of flue-cured tobacco, increase the net photosynthetic rate, stomatal conductance and transpiration rate, effectively reduce the carbon dioxide concentration, and provide a better environment for the healthy growth of flue-cured tobacco.

[0019] 2. The present invention significantly enhances the antioxidant capacity of flue-cured tobacco, promotes the activity of carbon-nitrogen metabolic enzymes, optimizes carbon-nitrogen metabolism, and enhances the stress resistance of flue-cured tobacco by applying soluble organic carbon fertilizers. The application of soluble organic carbon fertilizers also improves the tissue structure of flue-cured tobacco leaves, increases the thickness of mesophyll, palisade tissue and spongy tissue, helps to improve the appearance quality, sensory quality and chemical composition of flue-cured tobacco, especially the increase in organic acid content, thereby improving the overall quality of flue-cured tobacco. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is the influence diagram of the soluble organic carbon fertilizer of the present invention on the SPAD value;

[0021] Figure 2 It is a diagram of the anatomical structure of tobacco leaf tissue under the soluble organic carbon fertilizer of the present invention;

[0022] Figure 3 is a graph showing the effect of the soluble organic carbon fertilizer of the present invention on the activity of antioxidant enzymes;

[0023] Figure 4 is a graph showing the effect of the soluble organic carbon fertilizer of the present invention on the content of malondialdehyde and hydrogen peroxide;

[0024] Figure 5 This is a graph showing the effect of the soluble organic carbon fertilizer of the present invention on the activities of carbon metabolism enzymes in tobacco leaves;

[0025] Figure 6 The invention relates to the influence of the soluble organic carbon fertilizer on the activities of nitrogen metabolism enzymes in tobacco leaves. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0027] The following examples are used to illustrate the present invention, but they cannot be used to limit the scope of protection of the present invention. The conditions in the examples can be further adjusted according to specific conditions. Simple improvements to the method of the present invention under the premise of the concept of the present invention belong to the scope of protection claimed in the present invention.

[0028] Example

[0029] In order to verify the promoting effect of soluble organic carbon fertilizer on flue-cured tobacco, improve its physiological and biochemical characteristics and enhance the quality of tobacco leaves, the present invention conducted an experimental study by means of field trials, and the specific implementation steps are as follows:

[0030] 1. Materials and Methods

[0031] 1.1. Test materials and test site overview

[0032] The tested flue-cured tobacco variety was Zhongyan 100, and the tested material was 'Li Li Zhu' organic carbon fertilizer (functional bacteria ≥ 0.2 billion / g, effective carbon (AOC) ≥ 5.0%, organic matter ≥ 45.0%), purchased from Fujian Oasis Biochemical Co., Ltd.

[0033] The experiment was carried out in Likou Town, Jia County, Pingdingshan City, Henan Province (33.91°N, 113.26°E, 180m above sea level, average annual temperature 14.5℃). The test soil type was brown soil. The basic physical and chemical properties were organic matter 18.69g / kg, alkaline nitrogen 69.22mg / kg, available phosphorus 37.70mg / kg, available potassium 105.30mg / kg, and pH 6.82.

[0034] Experimental design

[0035] A completely randomized block design was used, and a total of four treatments were set up in the experiment, namely CK (conventional fertilization), T1 (conventional fertilization + soluble organic carbon fertilizer 300kg / hm 2 ), T2 (conventional fertilization + soluble organic carbon fertilizer 600kg / hm 2 ) and T3 (conventional fertilization + soluble organic carbon fertilizer 900kg / hm 2 ), three plots were set for each treatment, each plot was 66.7m 2 Each plot planted 110 tobacco plants, and the conventional fertilizer was tobacco-specific fertilizer with a nitrogen application rate of 52.5 kg / hm2. 2 , N:P 2 O 5 :K 2 O=1:1.5:3, soluble organic carbon fertilizer and base fertilizer are applied at the same time before ridge formation, floating seedlings are adopted, and transplanting is carried out on April 25, 2024. Field cultivation and management measures are implemented in accordance with local high-quality tobacco production technical specifications, and harvesting and curing will be completed in mid-September.

[0036] 1.3. Determination indicators and methods

[0037] 1.3.1. Agronomic traits survey

[0038] At 30, 45, 60, 75 and 90 days after flue-cured tobacco transplanting, three representative tobacco plants were selected from each plot and investigated according to the standard "Investigation and Measurement Methods for Agronomic Characteristics of Tobacco" (YC / T 142-2010).

[0039] 1.3.2. Root morphology and root activity determination

[0040] 60 days after flue-cured tobacco transplanting, the roots of each treatment were scanned with LA-2400 multi-parameter root scanner to measure the total root length, root surface area, root volume, average diameter and number of root tips. The root activity was determined by the improved triphenyltetrazolium chloride (TTC) method.

[0041] 1.3.3. Determination of tobacco leaf photosynthetic parameters

[0042] At 30, 45, 60, 75 and 90 days after transplanting, the SPAD values ​​were measured at six points, including the base, middle and tip of the middle functional leaves of each treatment, using a SPAD-502plus portable chlorophyll meter (Konica Minolta, Japan), and the average value was calculated. At 60 days after transplanting, the photosynthetic parameters of the middle leaves of each treatment, including net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) and intercellular carbon dioxide concentration (Ci), were measured using a Li-6400 portable photosynthetic meter.

[0043] 1.3.4. Leaf tissue structure observation

[0044] 90 days after transplanting the flue-cured tobacco, fresh leaves from the same position of the middle leaves of the tobacco plant were taken and cut into small pieces of 1 cm. After fixation with 4% paraformaldehyde solution, the target area of ​​the tissue was selected using an Eclipse Ci-L camera microscope for 100x imaging. After the imaging was completed, Image-Pro Plus 6.0 analysis software was used to uniformly use millimeters as the standard unit, select 100x, and measure the thickness of the upper epidermis, lower epidermis, leaf thickness, mesophyll thickness, palisade tissue thickness, and spongy tissue in each slice.

[0045] 1.3.5. Determination of tobacco leaf physiological indicators

[0046] 30, 60, and 90 days after transplanting, the representative middle leaves of each treatment were selected, and the part between the second and third veins at the base of the leaf was taken. The leaves were washed with distilled water, dried, and the veins were removed. The leaves were stored in liquid nitrogen for testing. The superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), nitrate reductase (NR), nitrite reductase (NIR), sucrose synthase (SS), sucrose phosphate synthase (SPS), malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 The kit was purchased from Beijing Solebow Technology Co., Ltd., and all operation steps were carried out strictly according to the kit instructions.

[0047] 1.3.6 Evaluation of the appearance quality of flue-cured tobacco leaves

[0048] After the tobacco leaves matured, the leaves collected in the test were individually braided and baked, and the appearance quality of the middle tobacco leaf samples after different treatments was evaluated in accordance with GB 2635-1992 "Flue-cured Tobacco".

[0049] 1.3.7. Sensory quality evaluation of flue-cured tobacco leaves

[0050] Based on the standard YC / T 138-1988 sensory evaluation method for tobacco and tobacco products, a 9-point sensory quality evaluation method for flue-cured tobacco was formed. Scores were given according to 10 individual indicators, including aroma quality, aroma quantity, concentration, softness, aftertaste, odor, irritation, strength, combustibility and ash content. The total score = (aroma quality + aroma quantity) × 2.3 + concentration × 1.5 + softness + aftertaste + odor + irritation.

[0051] 1.3.8. Determination of conventional chemical components of flue-cured tobacco leaves

[0052] The C3F of each treatment after curing was taken for testing, and the conventional chemical components of the cured tobacco leaves were determined with reference to YC / T 468-2013, YC / T 161-2002, YC / T 159-2002, YC / T 216-2013 and YC / T 166-2003.

[0053] Data processing and analysis

[0054] SPSS27.0 was used to analyze the data, and one-way analysis of variance (One-Way) and Duncan's multiple comparisons were used for significant difference tests. The significance level was P < 0.05, and GraphPad Prism10 was used to draw charts.

[0055] 2. Results and Analysis

[0056] 2.1. Effects of soluble organic carbon fertilizer on agronomic traits

[0057] The agronomic traits of different soluble organic carbon fertilizer application rates are shown in Table 1. At 30 days after tobacco transplanting, compared with the CK treatment, the agronomic traits of each soluble organic carbon fertilizer application treatment were improved, among which the plant height of T1, T2, and T3 treatments increased by 5.67%, 17.14%, and 7.86% respectively compared with the CK treatment; at 45 days after tobacco transplanting, compared with the CK treatment, the agronomic traits of T2 treatment increased the most, except for the maximum leaf width, the other indicators were significantly different from those of CK treatment; at 60 days after tobacco transplanting, except for the internode distance, the agronomic traits of T2 treatment were higher than those of CK treatment All of them were significantly improved, among which the plant height, stem girth and maximum leaf area increased by 49.41%, 43.02% and 52.63% compared with the CK treatment, respectively; 75 days after tobacco transplanting, compared with the CK treatment, with the increase of soluble organic carbon fertilizer application, the agronomic trait parameter values ​​of each treatment showed a trend of first increasing and then decreasing, with the T2 treatment having the largest value; 90 days after tobacco transplanting, compared with the CK treatment, the agronomic trait parameter values ​​of each treatment with soluble organic carbon fertilizer application increased, but the differences among the treatments were not obvious. Overall, the application of soluble organic carbon fertilizer can promote the growth of flue-cured tobacco, and the T2 treatment has the best growth.

[0058] Table 1 Effects of soluble organic carbon fertilizer on agronomic traits

[0059]

[0060]

[0061] Note: Different lowercase letters in the table indicate significant differences among treatments (P<0.05), the same below.

[0062] 2.2. Effects of soluble organic carbon fertilizer on root-related parameters

[0063] As shown in Table 2, compared with the CK treatment, the total root length, root surface area, root volume, average diameter and number of root tips of each treatment showed a trend of first increasing and then decreasing with the increase of soluble organic carbon fertilizer application. There were significant differences between T1, T2 and T3 and the CK treatment, and the T2 treatment was the largest and the CK treatment was the smallest. The total root length, root surface area, root volume, average diameter and number of root tips in the T2 treatment increased by 85.96%, 47.39%, 78.87%, 48.15% and 41.11% respectively compared with the CK treatment. Compared with the CK treatment, the root activity of each treatment with soluble organic carbon fertilizer was enhanced, among which the T2 treatment was the largest. The root activity of the T1, T2 and T3 treatments increased by 31.61%, 53.04% and 30.24% respectively compared with the CK treatment. It can be seen that the application of soluble organic carbon fertilizer can promote the growth and development of tobacco roots to a certain extent.

[0064] Table 2 Effects of soluble organic carbon fertilizer on root morphology

[0065]

[0066]

[0067] Effects of soluble organic carbon fertilizer on gas exchange parameters

[0068] 2.3.1. Effect of soluble organic carbon fertilizer on SPAD value

[0069] like Figure 1 (Note: Different lowercase letters in the figure column indicate significant differences among treatments (P<0.05). Figure 2-6 The SPAD values ​​of the treatments with soluble organic carbon fertilizers increased compared with the CK treatment at the same time, with the T2 treatment showing the highest value. At 90 days after transplanting, there was no significant difference in the SPAD values ​​among the treatments, and the values ​​were lower than those in other periods, indicating that chlorophyll began to degrade during this period and tobacco leaves gradually turned yellow.

[0070] 2.3.2. Effects of soluble organic carbon fertilizer on photosynthetic parameters

[0071] As shown in Table 3, compared with the CK treatment, the Pn, Gs and Tr of each treatment with soluble organic carbon fertilizer increased, with the T2 treatment showing the largest increase. There were significant differences between T2 and CK treatments, and the Pn, Gs and Tr of T2 treatment increased by 7.69%, 82.35% and 48.96% respectively compared with the CK treatment. The Ci was the largest in the CK treatment and the smallest in the T2 treatment. There were significant differences among the T1, T2, T3 and CK treatments, and the T1, T2 and T3 decreased by 40.03%, 61.99% and 47.17% respectively compared with the CK treatment.

[0072] Table 3 Effects of soluble organic carbon fertilizer on photosynthetic physiological parameters of tobacco

[0073]

[0074] 2.4. Effects of soluble organic carbon fertilizer on tobacco leaf tissue structure

[0075] like Figure 6 As shown, the basic anatomical structure of tobacco leaves treated differently is the same; the upper and lower epidermis are composed of a single layer of cells, with a layer of palisade tissue, 2 to 3 layers of spongy tissue, and glandular hairs on the epidermis.

[0076] As shown in Table 4, the thickness of the upper and lower epidermis was the thickest in T3 treatment and the thinnest in T1 treatment. Compared with the CK treatment, the thickness of leaves and mesophyll in the treatment with soluble organic carbon fertilizer increased, with the largest increase in T2 treatment, which increased by 47.21% and 53.31% respectively compared with the CK treatment. With the increase of soluble organic carbon fertilizer application, the thickness of palisade tissue and spongy tissue showed a trend of first increasing and then decreasing. The thickness of palisade tissue in T1, T2 and T3 treatments increased by 7.92%, 44.05% and 20.72% respectively compared with the CK treatment, and the thickness of spongy tissue in T1, T2 and T3 treatments increased by 27.75%, 65.67% and 4.42% respectively compared with the CK treatment.

[0077] Table 4 Effects of soluble organic carbon fertilizer on tobacco leaf tissue structure

[0078]

[0079] 2.5. Effect of soluble organic carbon fertilizer on tobacco leaf stress resistance

[0080] 2.5.1. Effects of soluble organic carbon fertilizer on antioxidant enzyme activities

[0081] Antioxidant enzyme activities under different soluble organic carbon fertilizer application rates Figure 2 As shown in the figure, 30 days after tobacco transplanting, compared with the CK treatment, the antioxidant enzyme activities of each treatment with soluble organic carbon fertilizer were increased; 60 days after tobacco transplanting, with the increase of soluble organic carbon fertilizer application, the antioxidant enzyme activity showed a trend of first increasing and then decreasing, with the highest in T2 treatment, which was significantly different from the CK treatment. The SOD, POD and CAT activities of T2 treatment were increased by 37.71%, 56.73% and 49.18% respectively compared with the CK treatment; 90 days after tobacco transplanting, compared with the CK treatment, the antioxidant enzyme activities of each treatment with soluble organic carbon fertilizer were increased, with the highest in T2 treatment.

[0082] 2.5.2. Effect of soluble organic carbon fertilizer on MDA and H 2 O 2 The influence of content

[0083] like Figure 3 As shown, compared with the CK treatment during the same period, the MDA and H202 contents of the treatments with soluble organic carbon fertilizer decreased to varying degrees, among which the T2 treatment had the largest decrease, and the difference between it and the CK treatment was significant. At 60 days after transplanting, the MDA and H202 contents of the T2 treatment were 29.39% and 34.81% lower than those of the CK treatment, respectively; at 90 days after transplanting, the MDA and H202 contents of each treatment were significantly higher than before.

[0084] 2.6. Effects of soluble organic carbon fertilizer on the activities of carbon metabolism enzymes in tobacco leaves

[0085] like Figure 4 As shown in the data, the application of soluble organic carbon fertilizer can increase the activity of SS and SPS. With the increase of the application amount of soluble organic carbon fertilizer, the SS activity showed a trend of first increasing and then decreasing, with the highest activity in T2 treatment. In each period, T2 was significantly increased by 27.75%, 30.11% and 32.32% compared with the CK treatment, respectively. The SPS activity showed a trend of first increasing and then decreasing with the increase of the application amount of soluble organic carbon fertilizer, with the highest activity in T2 treatment. However, there was no significant difference between the soluble organic carbon fertilizer application treatment and the CK treatment.

[0086] 2.7. Effects of soluble organic carbon fertilizer on the activities of nitrogen metabolic enzymes in tobacco leaves

[0087] like Figure 5 As shown, in the three periods, the NR and NIR activities of the treatment with soluble organic carbon fertilizer were higher than those of the CK treatment. During the same period, the NR and NIR activities of the T2 treatment were the highest, and there were significant differences between the T2 treatment and the CK treatment, but there were no significant differences between the T1, T3 and CK treatments. 60 days after tobacco transplanting, the NR activities of the T1, T2 and T3 treatments increased by 32.57%, 53.42% and 36.16% respectively compared with the CK treatment, and the NIR activities increased by 16.50%, 27.09% and 9.57% respectively compared with the CK treatment, and the NR and NIR activities of each treatment were higher than those in other periods.

[0088] 2.8. Effect of soluble organic carbon fertilizer on the appearance quality of flue-cured tobacco leaves

[0089] As shown in Table 5, the middle leaves of each treatment showed consistency in color, maturity, structure and chroma; in terms of identity, only the CK treatment showed a slightly thinner thickness, while the other three treatments showed a medium thickness; compared with the T1, T2 and T3 treatments, the CK treatment had less oil in the tobacco leaves after curing.

[0090] Table 5 Effect of soluble organic carbon fertilizer on the appearance quality of flue-cured tobacco leaves

[0091]

[0092]

[0093] 2.9. Effects of soluble organic carbon fertilizer on sensory quality of flue-cured tobacco leaves

[0094] As shown in Table 6, the total scores of sensory quality of flue-cured tobacco leaves treated with different soluble organic carbon fertilizer application rates were T2>T1>T3>CK, among which the aroma quality, aroma quantity, concentration, softness, aftertaste, irritation, combustibility and ash content of T2 treatment were higher than those of CK treatment, but the impurity score of T3 treatment was higher than that of CK treatment. In general, the sensory quality score of C3F grade flue-cured tobacco leaves treated with T2 was the highest, and the tobacco leaves had the best quality.

[0095] Table 6 Effects of soluble organic carbon fertilizer on sensory quality of flue-cured tobacco leaves

[0096]

[0097] 2.10. Effects of soluble organic carbon fertilizer on conventional chemical components of flue-cured tobacco leaves

[0098] As shown in Table 7, the total sugar and reducing sugar contents of the treatments with soluble organic carbon fertilizers were increased, among which the total sugar and reducing sugar contents of T2 treatment were significantly increased by 23.14% and 19.16% respectively compared with those of CK treatment; the potassium and starch contents of T2 and T3 treatments were significantly increased compared with those of CK treatment, with T2 treatment having the highest content, which was increased by 33.54% and 38.85% respectively compared with CK treatment; the application of soluble organic carbon fertilizers can significantly increase the potassium-chlorine ratio and sugar-alkali ratio, among which the potassium-chlorine ratio of T2 treatment is within the range of high-quality tobacco.

[0099] Table 7 Effects of soluble organic carbon fertilizer on conventional chemical components of flue-cured tobacco leaves

[0100]

[0101]

[0102] 2.11. Effect of soluble organic carbon fertilizer on organic acid content in flue-cured tobacco leaves

[0103] As shown in Table 8, a total of 12 organic acids were detected in the flue-cured tobacco leaves, among which the contents of 9 organic acids were higher in the treatment with soluble organic carbon fertilizer than in the CK treatment. The contents of malonic acid, succinic acid, malic acid and citric acid in the treatment with soluble organic carbon fertilizer were significantly higher than those in the CK treatment. The contents of 9 organic acids in the T2 treatment were significantly higher than those in the CK treatment, among which the largest difference was malic acid, which was 9.70 mg / g higher than that in the CK treatment, followed by citric acid, which was 1.86 mg / g higher than that in the CK treatment, followed by oxalic acid, malonic acid, linoleic acid, succinic acid, tetradecanoic acid, vanillic acid and eicosanoic acid. The total amount of organic acids in the T1, T2 and T3 treatments were 5.70, 13.84 and 5.51 mg / g higher than that in the CK treatment, respectively.

[0104] Table 8 Effect of soluble organic carbon fertilizer on organic acid content in flue-cured tobacco leaves

[0105]

[0106]

[0107] 3. Conclusion

[0108] Compared with the control, the application of soluble organic carbon fertilizer can promote the growth and development of flue-cured tobacco, enhance stress resistance and carbon and nitrogen metabolism, which is manifested in improving agronomic traits, root morphology, root activity, photosynthesis, tissue structure, antioxidant enzymes, carbon and nitrogen metabolism enzyme activities, and reducing malondialdehyde and H 2 O 2 At the same time, applying soluble organic carbon fertilizer can also improve the quality of flue-cured tobacco, specifically by improving the appearance quality, making the sensory quality and chemical composition more coordinated, and increasing the organic acid content. 2 The soluble organic carbon fertilizer has the best effect, which can provide a basis for the promotion and application of organic carbon fertilizer on tobacco.

[0109] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the technical solution of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An application of a soluble organic carbon fertilizer to flue-cured tobacco, characterized in that: The application of the soluble organic carbon fertilizer can promote the growth and development of flue-cured tobacco, improve the physiological and biochemical characteristics of flue-cured tobacco and enhance the quality of flue-cured tobacco.

2. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application rate of the soluble organic carbon fertilizer is 300kg / hm 2 Up to 900kg / hm 2 .

3. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The soluble organic carbon fertilizer is a water-soluble fertilizer rich in effective carbon, and its effective carbon content is 20 times that of ordinary organic fertilizer.

4. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application of the soluble organic carbon fertilizer can promote the growth of the root system of flue-cured tobacco, increase the total root length, root surface area, root volume, the number of root tips, and improve the activity of the root system.

5. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application of the soluble organic carbon fertilizer can improve the photosynthesis efficiency of flue-cured tobacco, increase the net photosynthesis rate, stomatal conductance and transpiration rate, and reduce the carbon dioxide concentration.

6. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application of the soluble organic carbon fertilizer can enhance the antioxidant capacity of flue-cured tobacco, improve the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and reduce the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2).

7. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application of the soluble organic carbon fertilizer can promote the activity of carbon-nitrogen metabolic enzymes in flue-cured tobacco, increase the activity of sucrose synthase (SS) and sucrose phosphate synthase (SPS), and optimize carbon-nitrogen metabolism.

8. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application of the soluble organic carbon fertilizer can improve the leaf tissue structure of flue-cured tobacco, which is manifested in the increase of leaf flesh thickness, palisade tissue thickness and spongy tissue thickness.

9. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application of the soluble organic carbon fertilizer can improve the appearance quality, sensory quality and chemical composition of the flue-cured tobacco leaves, especially increase the organic acid content, which is manifested by the increase in the content of organic acids such as malonic acid, malic acid, and citric acid.

10. The use of the soluble organic carbon fertilizer on flue-cured tobacco according to claim 1, characterized in that: The application of the soluble organic carbon fertilizer can increase the sugar, reducing sugar and total sugar content of flue-cured tobacco, optimize the potassium-chlorine ratio and the sugar-alkali ratio, and improve the chemical composition and quality of tobacco leaves. The application of the soluble organic carbon fertilizer can increase the potassium and starch content of flue-cured tobacco and make the potassium-chlorine ratio of flue-cured tobacco reach the standard of high-quality tobacco.

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