PDX model tumor specimen implantation device

Abstract

The invention relates to the technical field of specimen implantation devices, in particular to a PDX model tumor specimen implantation device. After an operator fixes a mouse on an operating table, the device is placed on the operating table and is close to the mouse; the implanting cylinder is rotated to be aligned with the to-be-implanted position of the mouse, then the position and angle of the implanting cylinder are locked through the first locking component, and then the implanting cylinder and the piston rod are synchronously pushed to embed the tip into the to-be-implanted position of the mouse; in the pushing process, the pointed end punctures the skin of the mouse to form a wound, and synchronously drives the implanting cylinder to extend into the body of the mouse; the piston rod is pulled out, then the pointed end on the piston head is screwed off, the plug is installed on the piston head in a threaded mode, then the piston head is embedded into the implanting cylinder again, the specimen is placed into the implanting cylinder through the feeding port, then the piston rod is manually pushed, and therefore the specimen is implanted into the mouse body. After implantation is completed, the implantation cylinder is pulled out of the mouse body; the whole operation process is safer, more convenient and more efficient.

Description

Technical Field

[0001] The present invention relates to the technical field of specimen implantation devices, and in particular to a PDX model tumor specimen implantation device. Background Art

[0002] Patient-derived xenograft (PDX) is an animal model that reproduces the biological characteristics and microenvironment of the patient's tumor by directly transplanting the patient's tumor tissue into immunodeficient mice. Compared with other models (such as cell, organoid, and cell-derived xenograft animal models), PDX models can highly retain the inherent characteristics of patient tumors, including tumor heterogeneity, genomic stability, and correlation with clinical drug response; currently, the industry mostly constructs PDX models through surgery, which requires a certain degree of incision of the mouse skin or peritoneum, and the specimen is implanted into the mouse with handheld forceps and then sutured. The whole process is cumbersome, time-consuming, labor-intensive, and traumatic, and it is easy to cause the death of mice due to surgery-related complications during or after surgery. Summary of the invention

[0003] The main purpose of the present invention is to provide a PDX model tumor specimen implantation device, which aims to solve the problem that the current process of constructing a PDX model through surgery is relatively cumbersome, time-consuming, labor-intensive and traumatic.

[0004] To achieve the above purpose, the technical solution proposed by the present invention is:

[0005] A PDX model tumor specimen implantation device comprises an implantation tube, a piston head, a piston rod, a plug, a tip, a connecting frame, a support frame, a universal ball, a first locking component, a support column and a base; the two ends of the implantation tube are open; the piston head is connected to one end of the piston rod; the tip is detachably connected to the end of the piston head away from the piston rod; the tip is conical, and the maximum diameter of the tip is consistent with the diameter of the piston head; the piston head is slidably embedded in the implantation tube; the end of the piston rod away from the tip extends out of the open end of the implantation tube; the implantation tube close to the tip One side of the pointed tip is provided with a feed port connected to the interior of the implant tube; the outer wall of the implant tube is connected to a feed tube covering the feed port; the plug is detachably connected to the end of the piston head facing away from the piston rod; the implant tube is detachably connected to the connecting frame; the connecting frame is slidably connected to the support frame; the universal ball is connected to the support frame; the universal ball is movably connected to the top of the support column; the first locking component is arranged on the support column to lock the universal ball, thereby locking the angle of the implant tube relative to the support column; the base is arranged at the bottom of the support column.

[0006] Preferably, a first connecting block and a first inner cavity below the first connecting block are formed on the top of the support column; a second connecting block is arranged inside the support column below the first inner cavity; a second inner cavity is formed on the support column below the second connecting block; an arc-shaped through hole is formed through the first connecting block; and the universal ball is movably embedded in the arc-shaped through hole.

[0007] Preferably, the inner wall of the arc-shaped through hole is in close contact with the outer wall of the universal ball; the first connecting block includes a first outer wall and a second outer wall parallel to each other; the two ends of the arc-shaped through hole pass through the first outer wall and the second outer wall respectively; the first outer wall and the second outer wall are symmetrical about the center of the universal ball; the center of the universal ball is on the vertical center axis of the arc-shaped through hole; the top of the universal ball extends upward from the first outer wall; the bottom of the universal ball extends downward from the second outer wall, and the bottom of the universal ball is in the first inner cavity.

[0008] Preferably, the first locking component includes a locking block, a first spring, a first sliding rod and a second sliding rod; the locking block and the first spring are both arranged in the first inner cavity; the first sliding rod and the second sliding rod are both slidably penetrated by the second connecting block; the first sliding rod is parallel to the second sliding rod, and the first sliding rod and the second sliding rod are symmetrical about the center of the universal ball; one end of the first sliding rod is connected to the locking block, and the other end of the first sliding rod extends into the second inner cavity; one end of the first spring is connected to the side of the second connecting block facing the first connecting block, and the other end of the first spring is connected to the side of the locking block away from the universal ball; the first spring is always in a compressed state; a groove is provided on the side of the locking block facing the universal ball; the elastic force of the first spring causes the groove to fit and abut against the bottom of the universal ball to fix the universal ball.

[0009] Preferably, the first locking component also includes a connecting plate and an electromagnet; the connecting plate and the electromagnet are both located in the second inner cavity; the first slide bar and the second slide bar are both connected to the connecting plate; the connecting plate is parallel to the first outer wall; the electromagnet is arranged on the side of the connecting plate away from the second connecting block; the electromagnet is used to attract the connecting plate; when the electromagnet attracts the connecting plate, the locking block and the universal ball are separated; the first spring is located between the first slide bar and the second slide bar.

[0010] Preferably, it also includes a control switch and a battery; the control switch is arranged on the outer wall of the support column; the battery is arranged in the second inner cavity; the battery is used to supply power to the electromagnet; the control switch is used to control the start and stop of the electromagnet.

[0011] Preferably, it also includes a second locking component; the connecting frame includes a first connecting arm, a second connecting arm, a third sliding rod and a fourth sliding rod; the first connecting arm is parallel to the second connecting arm; the third sliding rod is parallel to the fourth sliding rod; the two ends of the third sliding rod are respectively connected to the first connecting arm and the second connecting arm; the two ends of the fourth sliding rod are respectively connected to the first connecting arm and the second connecting arm; a first through hole is opened in the middle of the first connecting arm, and a second through hole is opened in the middle of the second connecting arm; the first through hole and the second through hole are directly opposite to each other; the first connecting arm is perpendicular to the third sliding rod; the implant tube is used to pass through the first through hole and the second through hole in sequence; the second locking component is used to fix the implant tube to the connecting frame.

[0012] Preferably, the support frame includes a connecting rod, a third connecting block, a fourth connecting block and a fifth connecting block; the fourth connecting block and the fifth connecting block are respectively connected to the two ends of the third connecting block; the fourth connecting block and the fifth connecting block are opposite to each other; one end of the connecting rod is connected to the side of the third connecting block away from the fourth connecting block; the other end of the connecting rod is connected to the universal ball; the third sliding rod is slidably provided in the fourth connecting block; the fourth sliding rod is slidably provided in the fifth connecting block.

[0013] Preferably, it also includes a reinforcement rod, a ring, a threaded rod and a screw cap; the third connecting block is also connected to a third connecting arm; the third connecting arm is parallel to the third sliding rod; the central axis of the implant tube is parallel to the third sliding rod; the ring is slidably mounted on the support column; one end of the reinforcement rod is universally hinged to the end of the third connecting arm away from the third connecting block; the other end of the reinforcement rod is universally hinged to the outer wall of the ring; the ring is provided with a threaded hole; the threaded rod is screwed in the threaded hole; the screw cap is provided at the end of the threaded rod away from the support column; the threaded rod is used to abut the outer wall of the support column to fix the ring.

[0014] Preferably, the second locking component includes a first connecting column, a second connecting column, a rotating shaft, a fifth sliding rod, a second spring, a first baffle and a second baffle; the outer wall of the implant tube is sleeved with an annular baffle; the first connecting column and the second connecting column are both arranged on the top of the first connecting arm; the first connecting column is parallel to the second connecting column; the two ends of the rotating shaft are rotatably connected to the first connecting column and the second connecting column respectively; the rotating shaft is perpendicular to the third sliding rod, and the rotating shaft is perpendicular to the supporting column; the fifth sliding rod is slidably penetrated by the rotating shaft, and the fifth sliding rod is perpendicular to the rotating shaft; The first baffle plate and the second baffle plate are respectively vertically connected to the two ends of the fifth slide bar, and the first baffle plate and the second baffle plate are respectively located on both sides of the rotating shaft; the second spring is sleeved on the fifth slide bar; one end of the second spring is connected to the rotating shaft, and the other end of the second spring is connected to the second baffle plate; the second spring is always in a compressed state; the elastic force of the second spring makes the first baffle plate tend to abut against the rotating shaft; the first baffle plate is used to abut against the annular baffle plate, so that the annular baffle plate abuts against the first connecting arm, so as to fix the implant tube to the connecting frame.

[0015] Compared with the prior art, the present invention has at least the following beneficial effects:

[0016] The PDX model tumor specimen implantation device proposed in the present invention can implant specimens into mice more safely, conveniently and quickly. When operating the device, the operator first fixes the mouse behind the operating table, and then places the device on the operating table close to the mouse. The implantation tube is rotated to align the implantation tube with the position to be implanted in the mouse, and then the position and angle of the implantation tube are locked by the first locking component. Then, the implantation tube and the piston rod are synchronously pushed to embed the tip into the position to be implanted in the mouse (because the connecting frame is slidably connected to the supporting frame, the implantation tube can be pushed forward or backward while maintaining the angle of the implantation tube relative to the supporting column). The position to be implanted in the mouse can be the abdominal cavity or subcutaneous. During the pushing process, the tip will pierce the The mouse skin is broken to form a wound, and the implant tube is simultaneously driven into the mouse body; then the movement of the implant tube is stopped and the piston rod is pulled back, and then the pointed head on the piston head is unscrewed, and the plug thread is installed on the piston head, and then the piston head is re-embedded in the implant tube, and the piston head is located behind the feed port, and then the specimen is placed into the implant tube through the feed port, and then the piston rod is manually pushed to push the specimen forward, thereby pushing the specimen out of the end of the implant tube that is in the mouse body to be implanted into the mouse body; after the implantation is completed, the implant tube and the piston rod are pulled in reverse to pull the implant tube out of the mouse body; the entire operation process is safer, more convenient and more efficient than the traditional PDX model tumor specimen implantation operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0018] Figure 1 This is a schematic structural diagram of an embodiment of a PDX model tumor specimen implantation device proposed by the present invention;

[0019] Figure 2 for Figure 1 A magnified schematic diagram of the details in the middle;

[0020] Figure 3 for Figure 1 A magnified schematic diagram of the details at B in the middle;

[0021] Figure 4 This is a schematic diagram of the structure of the implantation tube of one embodiment of the PDX model tumor specimen implantation device proposed by the present invention;

[0022] Figure 5 This is a schematic structural diagram of a connecting frame and a supporting frame of an embodiment of a PDX model tumor specimen implantation device proposed by the present invention;

[0023] Figure 6 This is a schematic structural diagram of the piston rod and tip-related components of an embodiment of the PDX model tumor specimen implantation device proposed by the present invention;

[0024] Figure 7 This is a schematic structural diagram of the piston rod and plug-related components of an embodiment of the PDX model tumor specimen implantation device proposed by the present invention.

[0025] Description of reference numerals:

[0026] 110, implant tube; 120, piston rod; 130, end cap; 140, pointed head; 150, first connecting arm; 160, second connecting arm; 170, third slide bar; 180, fourth slide bar; 190, fourth connecting block; 210, fifth connecting block; 220, third connecting block; 230, third connecting arm; 240, connecting rod; 250, universal ball; 260, supporting column; 270, base; 280, reinforcing rod; 290, collar; 310, threaded rod; 320, screw cap; 330, feeding barrel; 340, annular baffle; 350, first connecting column; 360, rotating shaft; 370, fifth slide bar; 380, second spring; 390, first baffle; 410, second baffle; 420, plug; 430, piston head; 440, rubber sleeve; 450, feeding port; 460, arc-shaped through hole; 470, first connecting block; 480, first outer wall; 490, second outer wall; 510, first inner cavity; 520, second inner cavity; 530, second connecting block; 540, locking block; 550, groove; 560, first spring; 570, first slide bar; 580, second slide bar; 590, connecting plate; 610, electromagnet.

[0027] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0030] In addition, in the present invention, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0031] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] In addition, the technical solutions between the various embodiments of the present invention can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0033] The present invention provides a PDX model tumor specimen implantation device.

[0034] As attached Figure 1 -Attached Figure 7 As shown, in one embodiment of a PDX model tumor specimen implantation device proposed by the present invention, the PDX model tumor specimen implantation device includes an implantation tube 110, a piston head 430, a piston rod 120, a plug 420, a tip 140, a connecting frame, a support frame, a universal ball 250, a first locking component, a support column 260 and a base 270; both ends of the implantation tube 110 are open; the piston head 430 is connected to one end of the piston rod 120; the tip 140 is detachably connected to the end of the piston head 430 away from the piston rod 120; the tip 140 is conical, and the maximum diameter of the tip 140 is consistent with the diameter of the piston head 430 (because the size of the tumor specimen is generally 5-8mm, and the size of the mouse incision is generally 1cm, the maximum diameter of the tip 140 in this embodiment is 5-8mm, and the maximum diameter of the tip 140 is consistent with the diameter of the implantation tube 1 10 has the same inner diameter); the piston head 430 is slidably embedded in the implant tube 110; the end of the piston rod 120 away from the tip 140 extends out of the opening of the implant tube 110; a feed port 450 connected to the interior of the implant tube 110 is provided on the side of the implant tube 110 close to the tip 140; the outer wall of the implant tube 110 is connected to the feed tube 330 with the feed port 450 covered; the plug 420 can be detachably connected to the end of the piston head 430 away from the piston rod 120; the implant tube 110 is detachably connected to the connecting frame; the connecting frame is slidably connected to the support frame; the universal ball 250 is connected to the support frame; the universal ball 250 is movably connected to the top of the support column 260; the first locking component is arranged on the support column 260 to lock the universal ball 250, thereby locking the angle of the implant tube 110 relative to the support column 260; the bottom of the support column 260 is provided with a base 270.

[0035] Specifically, as attached Figure 1 , 4 , 6 and 7, a threaded hole (not shown) is provided at one end of the piston head 430 away from the piston rod 120, a first threaded rod (not numbered) capable of being screwed into the threaded hole is provided at the flat end of the pointed head 140, and a second threaded rod (not numbered) capable of being screwed into the threaded hole is provided at one side of the plug 420; an end of the piston rod 120 extending out of the implant cylinder 110 is detachably provided with an end cap 130; the piston rod 120 is also sleeved with a rubber sleeve 440; the rubber sleeve 440 is slidably abutted against the inner wall of the implant cylinder 110; when the pointed head 140 just extends out of one end of the implant cylinder (as shown in the attached Figure 4 As shown in FIG. 1 , the rubber sleeve 440 is located on a side of the feed inlet 450 close to the end cap 130 (ie, the feed inlet 450 is located between the piston head 430 and the rubber sleeve 440 ); both the piston head 430 and the rubber sleeve 440 are made of rubber.

[0036] The PDX model tumor specimen implantation device proposed in the present invention can implant specimens into mice more safely, conveniently and quickly. When operating the device, the operator first fixes the mouse on the operating table, and then places the device on the operating table close to the mouse. The implantation tube 110 is rotated to align the implantation tube 110 with the position to be implanted in the mouse, and then the position and angle of the implantation tube 110 are locked by the first locking component. Then, the implantation tube 110 and the piston rod 120 are synchronously pushed to embed the pointed tip 140 into the position to be implanted in the mouse (because the connecting frame is slidably connected to the supporting frame, the implantation tube 110 can be pushed forward or backward while maintaining the angle of the implantation tube 110 relative to the supporting column 260). The position to be implanted in the mouse can be the abdominal cavity or subcutaneous. During the pushing process, the pointed tip 140 will pierce the mouse skin to form a wound, and synchronously drive the implantation tube 110 to extend into the small mouse body; then stop moving the implant cylinder 110 and pull out the piston rod 120 backwards, then unscrew the pointed head 140 on the piston head 430, and thread the plug 420 on the piston head 430, and then reinsert the piston head 430 into the implant cylinder 110, and make the piston head 430 behind the feed port 450, and then put the specimen into the implant cylinder 110 through the feed port 450, and then manually push the piston rod 120 to push the specimen forward, and then push the specimen out of the end of the implant cylinder 110 that is in the mouse body to be implanted into the mouse body; after the implantation is completed, pull the implant cylinder 110 and the piston rod 120 in the opposite direction to pull the implant cylinder 110 out of the mouse body; the entire operation process is safer, more convenient and more efficient than the traditional PDX model tumor specimen implantation operation, because the pointed head is used to pierce the mouse, the incision is smaller than that of traditional surgery.

[0037] In addition, if attached Figure 2As shown, a first connecting block 470 is formed on the top of the support column 260, and a first inner cavity 510 is located below the first connecting block 470; a second connecting block 530 is arranged inside the support column 260 below the first inner cavity 510; a second inner cavity 520 is formed on the support column 260 below the second connecting block 530; an arc-shaped through hole 460 is opened through the first connecting block 470; and the universal ball 250 is movably embedded in the arc-shaped through hole 460.

[0038] At the same time, the inner wall of the arc through hole 460 is in close contact with the outer wall of the universal ball 250; the first connection block 470 includes a first outer wall 480 and a second outer wall 490 which are parallel to each other; the two ends of the arc through hole 460 respectively penetrate the first outer wall 480 and the second outer wall 490; the first outer wall 480 and the second outer wall 490 are symmetrical with the center of the universal ball 250; the center of the universal ball 250 is located on the vertical central axis of the arc through hole 460; the top of the universal ball 250 extends upward from the first outer wall 480; the bottom of the universal ball 250 extends downward from the second outer wall 490, and the bottom of the universal ball 250 is located in the first inner cavity 510. Through the above technical solution, the specific structure of the universal ball 250 universally connected to the support column 260 is improved.

[0039] In addition, the first locking component includes a locking block 540, a first spring 560, a first slide bar 570 and a second slide bar 580; the locking block 540 and the first spring 560 are both arranged in the first inner cavity 510; the first slide bar 570 and the second slide bar 580 are both slidably penetrated in the second connecting block 530; the first slide bar 570 is parallel to the second slide bar 580, and the first slide bar 570 and the second slide bar 580 are symmetrical about the center of the universal ball 250; one end of the first slide bar 570 is connected to the locking block 540, and the other end of the first slide bar 570 extends into the second inner cavity 520; one end of the second slide bar 580 is connected to the locking block 540, and the other end of the first slide bar 570 extends into the second inner cavity 520; Connected to the locking block 540, the other end of the second sliding rod 580 extends into the second inner cavity 520; one end of the first spring 560 is connected to the side of the second connecting block 530 facing the first connecting block 470, and the other end of the first spring 560 is connected to the side of the locking block 540 facing away from the universal ball 250; the first spring 560 is always in a compressed state; a groove 550 is provided on the side of the locking block 540 facing the universal ball 250 (a rubber anti-slip layer is provided in the groove 550); the elastic force of the first spring 560 makes the groove 550 fit and abut against the bottom of the universal ball 250 to fix the universal ball 250.

[0040] At the same time, the first locking component also includes a connecting plate 590 and an electromagnet 610; the connecting plate 590 and the electromagnet 610 are both in the second inner cavity 520; the first slide bar 570 and the second slide bar 580 are both connected to the connecting plate 590; the connecting plate 590 is parallel to the first outer wall 480; the electromagnet 610 is arranged on the side of the connecting plate 590 away from the second connecting block 530; the electromagnet 610 is used to attract the connecting plate 590; when the electromagnet 610 attracts the connecting plate 590, the locking block 540 and the universal ball 250 are separated; the first spring 560 is between the first slide bar 570 and the second slide bar 580. The PDX model tumor specimen implantation device also includes a control switch and a battery; the control switch is arranged on the outer wall of the support column 260; the battery is arranged in the second inner cavity 520; the battery is used to power the electromagnet 610; the control switch is used to control the start and stop of the electromagnet 610.

[0041] Through the above technical scheme, the structure and function of the first locking component are further improved; when it is necessary to adjust the angle of the implant tube 110 relative to the support column 260 to align with the position to be implanted in the mouse, the electromagnet 610 is started by the control switch, thereby attracting the connecting plate 590 to separate the locking block 540 and the universal ball 250, and the angle of the implant tube 110 relative to the support column 260 can be normally rotated in a universal direction; after the adjustment is completed, the electromagnet 610 is stopped by the control switch, and under the action of the first spring 560, the locking block 540 abuts against the bottom of the universal ball 250 (specifically, the groove 550 abuts against the bottom of the universal ball 250) to fix the universal ball 250, thereby fixing the angle of the implant tube 110 relative to the support column 260.

[0042] In addition, if attached Figure 1 and attached Figure 5 As shown, the PDX model tumor specimen implantation device also includes a second locking component; the connecting frame includes a first connecting arm 150, a second connecting arm 160, a third sliding rod 170 and a fourth sliding rod 180; the first connecting arm 150 is parallel to the second connecting arm 160; the third sliding rod 170 is parallel to the fourth sliding rod 180; both ends of the third sliding rod 170 are respectively connected to the first connecting arm 150 and the second connecting arm 160; both ends of the fourth sliding rod 180 are respectively connected to the first connecting arm 150 and the second connecting arm 160; a first through hole is opened in the middle of the first connecting arm 150, and a second through hole is opened in the middle of the second connecting arm 160; the first through hole and the second through hole are directly opposite to each other; the first connecting arm 150 is perpendicular to the third sliding rod 170; the implant tube 110 is used to pass through the first through hole and the second through hole in sequence; the second locking component is used to fix the implant tube 110 to the connecting frame.

[0043] At the same time, the support frame includes a connecting rod 240, a third connecting block 220, a fourth connecting block 190 and a fifth connecting block 210; the fourth connecting block 190 and the fifth connecting block 210 are respectively connected to the two ends of the third connecting block 220; the fourth connecting block 190 and the fifth connecting block 210 are opposite to each other; one end of the connecting rod 240 is connected to the side of the third connecting block 220 away from the fourth connecting block 190; the other end of the connecting rod 240 is connected to the universal ball 250; the third sliding rod 170 is slidably penetrated through the fourth connecting block 190; the fourth sliding rod 180 is slidably penetrated through the fifth connecting block 210.

[0044] Through the above technical solution, the specific structure of the support frame and the connecting frame is improved. Since the third slide bar 170 is slidably penetrated in the fourth connecting block 190; the fourth slide bar 180 is slidably penetrated in the fifth connecting block 210, the connecting frame can slide as a whole relative to the support frame, thereby driving the implant tube 110 to move axially, so that the pointed head 140 can be embedded in the body of the mouse for specimen implantation.

[0045] In addition, if attached Figure 1 As shown, the PDX model tumor specimen implantation device also includes a reinforcement rod 280, a ring 290, a threaded rod 310 and a screw cap 320; the third connecting block 220 is also connected to the third connecting arm 230; the third connecting arm 230 is parallel to the third sliding rod 170; the central axis of the implant tube 110 is parallel to the third sliding rod 170; the ring 290 is slidably mounted on the support column 260; one end of the reinforcement rod 280 is universally hinged to the end of the third connecting arm 230 away from the third connecting block 220; the other end of the reinforcement rod 280 is universally hinged to the outer wall of the ring 290; the ring 290 is provided with a threaded hole; the threaded rod 310 is screwed in the threaded hole; the end of the threaded rod 310 away from the support column 260 is provided with a screw cap 320; the threaded rod 310 is used to abut the outer wall of the support column 260 to fix the ring 290.

[0046] Through the above technical solution, when adjusting the angle of the implant tube 110 relative to the support column 260, loosen the threaded rod 310 so that the ring 290 can slide freely vertically relative to the support column 260. Because the reinforcement rod 280, the ring 290 and the third connecting arm 230 are all universally hinged, the reinforcement rod 280 will not limit the rotation of the implant tube 110; when the angle adjustment of the implant tube 110 is completed, tighten the threaded rod 310 so that the threaded rod 310 abuts against the outer wall of the support column 260 to fix the position of the ring 290, thereby providing a more stable support effect for the support frame, the connecting frame and the implant tube 110 through the reinforcement rod 280.

[0047] At the same time, if attached Figure 3As shown, the second locking component includes a first connecting column 350, a second connecting column, a rotating shaft 360, a fifth sliding rod 370, a second spring 380, a first baffle 390 and a second baffle 410; the outer wall of the implant tube 110 is sleeved with an annular baffle 340; the first connecting column 350 and the second connecting column are both arranged at the top of the first connecting arm 150; the first connecting column 350 is parallel to the second connecting column; the two ends of the rotating shaft 360 are respectively rotatably connected to the first connecting column 350 and the second connecting column; the rotating shaft 360 is perpendicular to the third sliding rod 170, and the rotating shaft 360 is perpendicular to the support column 260; the fifth sliding rod 370 is slidably penetrated by the rotating shaft 360, and the fifth sliding rod 370 is perpendicular to the rotating shaft 3 60; the first baffle plate 390 and the second baffle plate 410 are respectively vertically connected to the two ends of the fifth slide bar 370, and the first baffle plate 390 and the second baffle plate 410 are respectively located on both sides of the rotating shaft 360; the second spring 380 is sleeved on the fifth slide bar 370; one end of the second spring 380 is connected to the rotating shaft 360, and the other end of the second spring 380 is connected to the second baffle plate 410; the second spring 380 is always in a compressed state; the elastic force of the second spring 380 makes the first baffle plate 390 have a tendency to abut against the rotating shaft 360; the first baffle plate 390 is used to abut against the annular baffle plate 340, so that the annular baffle plate 340 abuts against the first connecting arm 150, so as to fix the implant tube 110 to the connecting frame.

[0048] Through the above technical solution, the specific structure of the second locking component is improved. When the implant tube 110 needs to be locked and fixed to the connecting frame, the implant tube 110 is first passed through the first through hole and the second through hole in sequence, and the annular baffle 340 is close to and abuts against the first connecting arm 150, and then the second baffle 410 is pushed and the fifth sliding rod 370 is rotated to make the first baffle 390 on the side of the annular baffle 340 away from the first connecting arm 150, and then the second baffle 410 is released. Under the action, the first baffle plate 390 abuts against the annular baffle plate 340, so that the annular baffle plate 340 is tightly pressed against the first connecting arm 150, thereby fixing the implant tube 110 to the connecting frame; when it is necessary to loosen and disassemble the implant tube 110 (for example, to clean the implant tube 110), the second baffle plate 410 is pushed again and the fifth slide bar 370 is rotated, so that the first baffle plate 390 no longer abuts against the annular baffle plate 340, and is above the annular baffle plate 340, and the implant tube 110 can be pulled out from the connecting frame.

[0049] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A PDX model tumor specimen implantation device, characterized in that: It comprises an implant tube, a piston head, a piston rod, a plug, a tip, a connecting frame, a supporting frame, a universal ball, a first locking component, a supporting column and a base; both ends of the implant tube are open; the piston head is connected to one end of the piston rod; the tip is detachably connected to the end of the piston head away from the piston rod; the tip is conical, and the maximum diameter of the tip is consistent with the diameter of the piston head; The piston head is slidably embedded in the implant tube; the end of the piston rod away from the tip extends out of the opening of the implant tube; a feed port connected to the inside of the implant tube is opened on the side of the implant tube close to the tip; the outer wall of the implant tube is connected with a feed tube covering the feed port; the plug is detachably connected to the end of the piston head away from the piston rod; the implant tube is detachably connected to the connecting frame; the connecting frame is slidably connected to the support frame; the universal ball is connected to the support frame; the universal ball is movably connected to the top of the support column; the first locking component is arranged on the support column to lock the universal ball, thereby locking the angle of the implant tube relative to the support column; the base is arranged at the bottom of the support column.

2. A PDX model tumor specimen implantation device according to claim 1, characterized in that: A first connecting block and a first inner cavity below the first connecting block are formed on the top of the support column; a second connecting block is arranged inside the support column below the first inner cavity; a second inner cavity is formed on the support column below the second connecting block; an arc-shaped through hole is formed through the first connecting block; and the universal ball is movably embedded in the arc-shaped through hole.

3. A PDX model tumor specimen implantation device according to claim 2, characterized in that: The inner wall of the arc-shaped through hole is in close contact with the outer wall of the universal ball; the first connecting block includes a first outer wall and a second outer wall which are parallel to each other; the two ends of the arc-shaped through hole pass through the first outer wall and the second outer wall respectively; the first outer wall and the second outer wall are symmetrical with respect to the center of the universal ball; the center of the universal ball is located on the vertical central axis of the arc-shaped through hole; the top of the universal ball extends upward from the first outer wall; the bottom of the universal ball extends downward from the second outer wall, and the bottom of the universal ball is located in the first inner cavity.

4. A PDX model tumor specimen implantation device according to claim 3, characterized in that: The first locking component includes a locking block, a first spring, a first sliding rod and a second sliding rod; the locking block and the first spring are both arranged in the first inner cavity; the first sliding rod and the second sliding rod are both slidably penetrated by the second connecting block; the first sliding rod is parallel to the second sliding rod, and the first sliding rod and the second sliding rod are symmetrical about the center of the universal ball; one end of the first sliding rod is connected to the locking block, and the other end of the first sliding rod extends into the second inner cavity; one end of the first spring is connected to the side of the second connecting block facing the first connecting block, and the other end of the first spring is connected to the side of the locking block away from the universal ball; the first spring is always in a compressed state; a groove is provided on the side of the locking block facing the universal ball; the elastic force of the first spring causes the groove to fit and abut against the bottom of the universal ball to fix the universal ball.

5. A PDX model tumor specimen implantation device according to claim 4, characterized in that: The first locking component also includes a connecting plate and an electromagnet; the connecting plate and the electromagnet are both located in the second inner cavity; the first slide bar and the second slide bar are both connected to the connecting plate; the connecting plate is parallel to the first outer wall; the electromagnet is arranged on the side of the connecting plate away from the second connecting block; the electromagnet is used to attract the connecting plate; when the electromagnet attracts the connecting plate, the locking block and the universal ball are separated; the first spring is located between the first slide bar and the second slide bar.

6. A PDX model tumor specimen implantation device according to claim 5, characterized in that: It also includes a control switch and a battery; the control switch is arranged on the outer wall of the support column; the battery is arranged in the second inner cavity; the battery is used to supply power to the electromagnet; the control switch is used to control the start and stop of the electromagnet.

7. A PDX model tumor specimen implantation device according to claim 2, characterized in that: It also includes a second locking component; the connecting frame includes a first connecting arm, a second connecting arm, a third sliding rod and a fourth sliding rod; the first connecting arm is parallel to the second connecting arm; the third sliding rod is parallel to the fourth sliding rod; the two ends of the third sliding rod are respectively connected to the first connecting arm and the second connecting arm; the two ends of the fourth sliding rod are respectively connected to the first connecting arm and the second connecting arm; a first through hole is opened in the middle of the first connecting arm, and a second through hole is opened in the middle of the second connecting arm; the first through hole and the second through hole are directly opposite to each other; the first connecting arm is perpendicular to the third sliding rod; the implant tube is used to pass through the first through hole and the second through hole in sequence; the second locking component is used to fix the implant tube to the connecting frame.

8. A PDX model tumor specimen implantation device according to claim 7, characterized in that: The support frame includes a connecting rod, a third connecting block, a fourth connecting block and a fifth connecting block; the fourth connecting block and the fifth connecting block are respectively connected to the two ends of the third connecting block; the fourth connecting block and the fifth connecting block are opposite to each other; one end of the connecting rod is connected to the side of the third connecting block away from the fourth connecting block; the other end of the connecting rod is connected to the universal ball; the third sliding rod is slidably provided in the fourth connecting block; the fourth sliding rod is slidably provided in the fifth connecting block.

9. A PDX model tumor specimen implantation device according to claim 8, characterized in that: It also includes a reinforcement rod, a ring, a threaded rod and a screw cap; the third connecting block is also connected to a third connecting arm; the third connecting arm is parallel to the third sliding rod; the central axis of the implant tube is parallel to the third sliding rod; the ring is slidably mounted on the support column; one end of the reinforcement rod is universally hinged to the end of the third connecting arm away from the third connecting block; the other end of the reinforcement rod is universally hinged to the outer wall of the ring; the ring is provided with a threaded hole; the threaded rod is screwed in the threaded hole; the screw cap is provided at the end of the threaded rod away from the support column; the threaded rod is used to abut the outer wall of the support column to fix the ring.

10. A PDX model tumor specimen implantation device according to claim 7, characterized in that: The second locking component includes a first connecting column, a second connecting column, a rotating shaft, a fifth sliding rod, a second spring, a first baffle and a second baffle; the outer wall of the implant tube is provided with an annular baffle; the first connecting column and the second connecting column are both arranged on the top of the first connecting arm; the first connecting column is parallel to the second connecting column; the two ends of the rotating shaft are rotatably connected to the first connecting column and the second connecting column respectively; the rotating shaft is perpendicular to the third sliding rod, and the rotating shaft is perpendicular to the supporting column; the fifth sliding rod is slidably arranged on the rotating shaft, and the fifth sliding rod is perpendicular to the rotating shaft; the first The baffle plate and the second baffle plate are respectively vertically connected to the two ends of the fifth slide bar, and the first baffle plate and the second baffle plate are respectively located on both sides of the rotating shaft; the second spring is sleeved on the fifth slide bar; one end of the second spring is connected to the rotating shaft, and the other end of the second spring is connected to the second baffle plate; the second spring is always in a compressed state; the elastic force of the second spring makes the first baffle plate tend to abut against the rotating shaft; the first baffle plate is used to abut against the annular baffle plate, so that the annular baffle plate abuts against the first connecting arm, so as to fix the implant tube to the connecting frame.

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