Cryoprecipitation blood coagulation factor preparation instrument and preparation method based on siphonage

Abstract

The invention belongs to the technical field of blood product equipment, and particularly relates to a cryoprecipitation blood coagulation factor preparation instrument based on a siphonage method and a preparation method. The cryoprecipitation blood coagulation factor preparation instrument based on the siphoning method comprises a rack, a water bath assembly, a siphoning assembly and a measuring assembly, the water bath assembly comprises a water bath tank; the measuring assembly comprises a weighing box and a weighing device; the siphon assembly comprises a siphon driving device and a siphon; the invention mainly aims to provide a device for realizing automatic production and preparation of cryoprecipitated blood coagulation factors, the device is based on a siphonage principle of a standard, plasma can be effectively protected, the efficacy of the plasma can be maintained, and the original content needing a large amount of manual operation or supervision is realized through automatic equipment, so that the production efficiency is greatly improved. The preparation difficulty of the cryoprecipitation blood coagulation factor is effectively reduced, the preparation speed is increased, meanwhile, the frequency of contact with unnecessary environments such as a human body in the preparation process is effectively reduced, and the isolation effect of a blood product is enhanced.

Description

Technical Field

[0001] The invention belongs to the technical field of blood product equipment, and in particular relates to a cold precipitation coagulation factor preparation instrument and preparation method based on a siphon method. Background Art

[0002] Cryoprecipitated coagulation factor is a commonly used blood component in clinical practice. It is a routine blood product in various blood stations (component departments) and is widely used in various medical scenarios.

[0003] According to the "Technical Regulations for Blood Station Operation" and other standards, the siphon method is usually used to prepare cold precipitated coagulation factors. The general operation is: place a fresh frozen plasma bag (Bag A) in a constant temperature water bath, and another empty bag (Bag B) is suspended outside the water bath, lower than the plasma bag, forming a certain height difference between the two bags, and then connected through a catheter to form a siphon channel. After the frozen plasma in the frozen plasma bag melts, it is siphoned into the B bag. When it melts to 40 to 50 ml of plasma and sediment, the catheter is closed to block the siphon, and then the plasma and sediment are mixed to make cold precipitated coagulation factors (Bag A). Due to the special requirements of plasma products, each plasma band needs to be independently isolated, resulting in a very low efficiency of the above preparation process, and a large amount of manpower is required to monitor the siphon transfer process at any time, resulting in low overall production efficiency and high production costs. Summary of the invention

[0004] The purpose of the present invention is to provide a special device for realizing automated cold precipitation to prepare cold precipitated coagulation factor, which is used to reduce the production cost of users such as blood stations (component departments) and improve the preparation efficiency of cold precipitated coagulation factor.

[0005] To achieve the above purpose, the present invention adopts the following technical solution.

[0006] A cold precipitation coagulation factor preparation instrument based on the siphon method, comprising: a frame 1, a water bath component 2, a siphon component 3, and a measuring component 4;

[0007] The water bath assembly 2 includes a water bath tank 20 disposed on the inner frame 10, a water outlet hole is disposed on the side wall of the water bath tank 20, and a water inlet hole is disposed at the bottom;

[0008] The measuring assembly 4 includes a plurality of weighing boxes 40 disposed at the lower front side of the water bath 20 and a weighing device located at the bottom of the weighing boxes 40;

[0009] The siphon assembly 3 includes a siphon driving device 30 disposed at the front side of the water bath 20, and a siphon tube 31 for connecting the frozen plasma bag and the transfer bag;

[0010] The siphon driving device 30 includes: a rear mounting plate 32, a mounting box 33, a pressure plate guide block 34, a movable pressure plate 35, a cam shaft 36, a fixed cut-off plate 37, a front mounting plate 38, a cut-off door 39, a movable cut-off plate 390, a locking rod 41, and an electric push rod 42;

[0011] The rear mounting plate 32 is fixed on the frame 1, and a mounting opening 32a is provided on the rear mounting plate 32. The mounting box 33 is located at the rear side of the rear mounting plate, and the front side of the mounting box 33 is open and buckled at the rear side of the mounting opening 32a.

[0012] Two pressure plate guide blocks 34 are symmetrically spliced ​​to form a boxed structure and fixed in the installation box 33. A plurality of horizontal guide grooves 34a are provided inside the pressure plate guide block 34. A pressure plate extension opening 34b is provided in front of the horizontal guide groove 34a. Camshaft mounting holes 34c are provided at the top and bottom of the pressure plate guide block 34.

[0013] The camshaft 36 is rotatably inserted into the camshaft mounting hole 34c, and a plurality of cam portions 36a are provided on the camshaft 36;

[0014] A plurality of movable pressing plates 35 are arranged horizontally and are sleeved on the cam shaft 36 in sequence from the top and face each cam portion 36a; a hole is provided on the rear side of the movable pressing plate 35 and is sleeved on the cam portion 36a, and both sides are inserted into the horizontal guide grooves 34a on the pressing plate guide block 34, and the front side extends from the pressing plate extension opening 34b to form a strip-shaped extrusion portion 35a;

[0015] The fixed cut-off plate 37 is fixed on the pressure plate guide block 34 and is located at the middle position of the cam shaft 36. The fixed cut-off plate 37 divides the movable pressure plate 35 into two parts, the upper and lower parts. The cam portion 36a can push the upper and lower parts of the movable pressure plate 35 to push the siphon tube 31 forward from top to bottom in sequence during the directional rotation of the cam shaft 36.

[0016] The front mounting plate 38 is a frame structure and is buckled on the front side of the mounting opening 32a. The cut-off door 39 and the lock rod 41 are respectively arranged on the left and right sides of the mounting plate 38. The cut-off door 39 is connected to the front mounting plate 38 through a torsion spring and a door shaft.

[0017] The front mounting plate 38 is provided with a vertical mounting groove 38a for mounting the locking rod 41, and the middle of the upper and lower sides thereof is provided with a siphon tube clamping groove 38b for inserting the siphon tube 31;

[0018] The lock rod 41 can be inserted into the vertical installation groove 38a movably up and down, a lock hook 41a is provided on the front side of the lock rod 41, and a lock hole 38c for the lock hook 41a to pass through is provided on the front side of the vertical installation groove 38a. One end of the lock rod 41 extends out of the vertical installation groove 38a to form an operating portion 41b, and a lock buckle 39a that can be engaged with the lock hook 41a is provided on the inner side of the cut-off door 39. A compression spring is also sleeved on the lock rod 41, and one end of the compression spring abuts against the front installation plate 38 and pushes the lock rod 41 to have a unidirectional movement tendency so that the lock hook 41a is connected with the lock buckle 39a.

[0019] The movable cut-off plate 390 is installed on the inner side of the cut-off door 39 through a rotating shaft, and the connection side is close to the connection side of the cut-off door 39 and the front mounting plate 38. A spring piece 50 is provided between the front side of the movable cut-off plate 390 and the rear side of the cut-off door 39. When the cut-off door 39 is closed, the spring piece 50 can push the movable cut-off plate 390 to swing forward and press against the fixed cut-off plate 37 to cut off the siphon tube 31.

[0020] The electric push rod 42 is fixed on the front mounting plate 38 and is located on the side where the locking rod 41 is located. The electric push rod 42 can push the movable cutting plate 390 to swing backward to make it disengage from the fixed cutting plate 37 and stop cutting.

[0021] As a further improvement or preferred embodiment of the aforementioned cold precipitation coagulation factor preparation instrument based on the siphon method, the siphon drive device 30 also includes: a camshaft drive motor 330 arranged on the rear side of the installation box 33, which is used to connect the output shaft of the camshaft drive motor 330 and the transmission gear set 331 of the camshaft 36.

[0022] A further improvement or preferred embodiment of the aforementioned cold precipitation coagulation factor preparation instrument based on the siphon method also includes a water circulation component 5, which includes a constant temperature water tank, a heat exchanger, a condenser, a compressor, a water inlet pump, and a circulating pump; the compressor is connected to the condenser and the cooling side of the heat exchanger through a refrigerant pipeline to form a refrigeration circuit; the water inlet hole is connected to the constant temperature water tank through a water pipe and a water inlet pump, and the water outlet hole is connected to the constant temperature water tank through a water pipe; the hot side of the heat exchanger is connected to the constant temperature water tank through a circulating pump and pipelines on both sides.

[0023] A further improvement or preferred embodiment of the aforementioned cold precipitation coagulation factor preparation instrument based on the siphon method, the inner frame 10 includes a first mounting surface 10a and a second mounting surface 10b formed by a plurality of horizontal support rods, the second mounting surface 10b is located below the front side of the first mounting surface 10a; the bottom support rods of the first mounting surface 10a form a first mounting cavity 10c, and the bottom support rods of the second mounting surface 10b form a second mounting cavity 10d, the outer panel frame 11 is composed of panels located at the front, back, left and right sides and a top panel, the top panel is provided with an installation window 10e facing the first mounting surface 10a and the second mounting surface 10b.

[0024] A further improvement or preferred embodiment of the aforementioned cold precipitation coagulation factor preparation instrument based on the siphon method is characterized in that the water bath 20 is a long strip trough structure, a water outlet box 22 is provided on the rear side thereof, a drain basket 21 is provided inside the water outlet box 22, the front wall of the water outlet box 22 is shared with the rear wall of the water bath 20, and a plurality of water outlet holes 22a are evenly provided on the wall, and water inlet holes 20a are evenly provided on the bottom of the water bath 20; the constant temperature water tank is located in the second installation cavity 10d, and the heat exchanger, condenser, compressor, water inlet pump, and circulating pump are located in the first installation cavity 10c.

[0025] A further improvement or preferred embodiment of the aforementioned cold precipitation coagulation factor preparation instrument based on the siphon method, a plurality of siphon drive devices 30 and weighing boxes 40 are arranged in a linear array along the length direction of the water bath 20, and correspond one to one; frozen plasma bags are evenly arranged in the drain basket 21 along the length direction of the water bath 20, and transfer bags are respectively provided in the weighing boxes 40, and the transfer bags are connected to the frozen plasma bags through siphon tubes 31.

[0026] A further improvement or preferred embodiment of the aforementioned cold precipitation coagulation factor preparation instrument based on the siphon method, the frame 1 is used to support the entire device, including an inner frame 10 internally formed by connecting multiple support rods and an outer plate frame 11 located outside the inner frame 10; usually the inner frame 10 is composed of a lightweight aluminum alloy pre-bracket spliced ​​by bolts and connecting parts, and the outer plate frame 11 is composed of sheet metal stamping welding or plastic plate splicing.

[0027] The present application also provides a method for using a cryoprecipitated coagulation factor preparation instrument based on a siphon method, comprising the following steps:

[0028] Step 1, preparing low-temperature water for cold precipitation through a water circulation component or other means, and introducing the low-temperature water into a water bath, and starting the water circulation in the water bath after the water temperature stabilizes;

[0029] Step 2, after placing the double bag into a weighing box, weigh and record the total weight; take out the plasma bag and the transfer bag from the weighing box, place the frozen plasma bag in a water tank, place the transfer bag in the weighing box, and clamp the siphon tube in the siphon tube clamping groove (38b) on the front mounting plate (38), so that the middle part of the siphon tube passes through each strip extrusion part (35a) and the fixed cut-off plate (37);

[0030] Step 3, close the cut-off door (39), lock the cut-off door (39) by means of the lock hook (41a) on the lock rod (41) and the lock buckle (39a) on the cut-off door (39), and then open the stop clamp between the two blood bags;

[0031] Step 4, waiting for the frozen plasma bag to initially melt, then driving the camshaft (36) to rotate through the camshaft drive motor (330) or the rotary drive element, driving each strip-shaped extrusion portion (35a) to squeeze the adsorption tube from top to bottom and release it from top to bottom, using low-pressure adsorption to make the melted components in the frozen plasma bag flow from top to bottom along the adsorption tube, repeating until the components reach the transfer bag, using the liquid's own weight and height difference to produce a siphon effect, and turning off the camshaft drive motor (330) or the rotary drive element;

[0032] Step 5, start the electric push rod (42) to push the movable cut-off plate (390) to swing backward to separate it from the fixed cut-off plate (37);

[0033] Step 6: Use a weighing device to monitor the mass of the components in the empty bag to determine whether enough cold precipitated coagulation factors have been separated. When enough cold precipitated coagulation factors have been collected, close the electric push rod (42), release the movable cut-off plate (390), and the spring (50) pushes the movable cut-off plate (390) to swing forward and press against the fixed cut-off plate (37) to cut off the siphon tube (31);

[0034] Step 7: Lock the plasma bag or the plasma bag catheter with the tube clamp provided with the plasma bag; take out the plasma bag with cold precipitation formed in the water bath and the plasma transfer bag with cold precipitation formed in the weighing box, and disconnect the heat seal.

[0035] A further improvement or preferred implementation step of the method for using the aforementioned cold precipitation coagulation factor preparation instrument is that the temperature of the low-temperature water is constant within the range of 4°C ± 2°C. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a front view of a cryoprecipitated coagulation factor preparation apparatus;

[0037] Figure 2 is a side sectional view of a cryoprecipitated coagulation factor preparation instrument;

[0038] Figure 3 This is a top view of the interior of the cryoprecipitated coagulation factor preparation instrument;

[0039] Figure 4 This is a schematic diagram of the interior of a cryoprecipitated coagulation factor preparation instrument;

[0040] Figure 5 It is an assembly diagram of the siphon drive device.

[0041] The reference numerals include:

[0042] Frame 1, inner frame 10, first mounting surface 10a, second mounting surface 10b, first mounting cavity 10c, second mounting cavity 10d, mounting window 10e, outer plate frame 11, water bath assembly 2, water bath tank 20, drain basket 21, water inlet 20a, water outlet box 22, water outlet 22a, siphon assembly 3, siphon drive device 30, siphon tube 31, rear mounting plate 32, mounting box 33, pressure plate guide block 34, movable pressure plate 35, camshaft 36, fixed cut-off plate 37, front mounting plate 38, cut-off door 39, movable cut-off plate 390, locking rod 41, electric push rod 42, mounting opening 32a, horizontal guide groove 34a, pressure plate extension opening 34b, camshaft mounting hole 34c, cam portion 36a, strip extrusion portion 35a, vertical mounting groove 38a, siphon tube slot 38b, locking hole 38c, lock buckle 39a, lock hook 41a, camshaft drive motor 330, transmission gear set 331, measuring component 4, weighing box 40, spring 50. DETAILED DESCRIPTION

[0043] The present invention is described in detail below in conjunction with specific embodiments.

[0044] The main purpose of the present invention is to provide a device for realizing the automated production and preparation of cold precipitated coagulation factors. The device is based on the standardized siphon method principle, can effectively protect plasma, maintain its effectiveness, and can realize the contents that originally required a large amount of manual operation or supervision through automated equipment, thereby effectively reducing the difficulty of preparing cold precipitated coagulation factors and improving their preparation speed. At the same time, it can effectively reduce the frequency of contact with non-essential environments such as the human body during the preparation process, thereby enhancing the isolation effect of blood products.

[0045] The main structure of the cryoprecipitated coagulation factor preparation instrument based on the siphon method of the present application includes:

[0046] Frame 1, water bath component 2, siphon component 3, measuring component 4, water circulation component 5, control component;

[0047] The frame 1 is used to support the whole equipment, including an inner frame 10 formed by connecting a plurality of support rods and an outer plate frame 11 located outside the inner frame 10; the inner frame 10 is usually formed by light aluminum alloy pre-frames spliced ​​by bolts and connectors, and the outer plate frame 11 is formed by sheet metal stamping welding or plastic plate splicing;

[0048] The inner frame 10 is formed by a plurality of horizontal support rods to form a first mounting surface 10a, the lower front side of the first mounting surface 10a forms a second mounting surface 10b, the bottom support rods of the first mounting surface 10a form a first mounting cavity 10c, and the bottom support rods of the second mounting surface 10b form a second mounting cavity 10d, the outer frame 11 is formed by panels located at the front, back, left and right sides and a top panel, and the top panel is provided with a mounting window 10e facing the first mounting surface 10a and the second mounting surface 10b;

[0049] The water circulation assembly includes a constant temperature water tank, a heat exchanger, a condenser, a compressor, a water inlet pump, and a circulating pump; the compressor is connected to the condenser and the cooling side of the heat exchanger through a refrigerant pipeline to form a refrigeration circuit; the water inlet hole is connected to the constant temperature water tank through a water pipe and a water inlet pump, and the water outlet hole is connected to the constant temperature water tank through a water pipe; the hot side of the heat exchanger is connected to the constant temperature water tank through a circulating pump and pipelines on both sides.

[0050] The inner frame 10 includes a first mounting surface 10a and a second mounting surface 10b formed by a plurality of horizontal support rods, and the second mounting surface 10b is located below the front side of the first mounting surface 10a; the bottom support rods of the first mounting surface 10a form a first mounting cavity 10c, and the bottom support rods of the second mounting surface 10b form a second mounting cavity 10d. The outer panel frame 11 is composed of panels located at the front, back, left and right sides and a top panel, and the top panel is provided with an installation window 10e facing the first mounting surface 10a and the second mounting surface 10b.

[0051] The water bath 20 is a long strip trough structure, with a water outlet box 22 on the rear side, a drain basket 21 inside, the front wall of the water outlet box 22 is shared with the rear wall of the water bath 20, and a number of water inlet holes 20a are evenly arranged on the wall, and water outlet holes 22a are evenly arranged on the bottom of the water bath 20; the constant temperature water tank is located in the second installation cavity 10d, and the heat exchanger, condenser, compressor, water inlet pump, and circulating pump are located in the first installation cavity 10c.

[0052] The measuring assembly 4 includes a plurality of weighing boxes 40 disposed at the lower front side of the water bath 20 and a weighing device located at the bottom of the weighing boxes 40;

[0053] The weighing box is used to hold the transfer bag. To ensure the sanitary isolation effect of the plasma bag, each weighing box is designed independently. The weighing device at the bottom of the weighing box is used to detect the total amount of plasma transferred to the transfer bag, so as to control the timing of siphon cut-off. The weighing device generally uses pressure sensors and other equipment or components to carefully measure the amount.

[0054] The siphon assembly 3 includes a siphon driving device 30 disposed at the front side of the water bath 20, and a siphon tube 31 for connecting the frozen plasma bag and the transfer bag;

[0055] The siphon driving device 30 includes: a rear mounting plate 32, a mounting box 33, a pressure plate guide block 34, a movable pressure plate 35, a cam shaft 36, a fixed cut-off plate 37, a front mounting plate 38, a cut-off door 39, a movable cut-off plate 390, a locking rod 41, and an electric push rod 42;

[0056] The siphon driving device 30 further includes: a camshaft driving motor 330 disposed at the rear side of the mounting box 33 , and a transmission gear set 331 for connecting the output shaft of the camshaft driving motor 330 and the camshaft 36 .

[0057] The rear mounting plate 32 is fixed on the frame 1, and a mounting opening 32a is provided on the rear mounting plate 32. The mounting box 33 is located at the rear side of the rear mounting plate, and the front side of the mounting box 33 is open and buckled at the rear side of the mounting opening 32a.

[0058] Two pressure plate guide blocks 34 are symmetrically spliced ​​to form a boxed structure and fixed in the installation box 33. A plurality of horizontal guide grooves 34a are provided inside the pressure plate guide block 34. A pressure plate extension opening 34b is provided in front of the horizontal guide groove 34a. Camshaft mounting holes 34c are provided at the top and bottom of the pressure plate guide block 34.

[0059] The camshaft 36 is rotatably inserted into the camshaft mounting hole 34c, and a plurality of cam portions 36a are provided on the camshaft 36;

[0060] A plurality of movable pressing plates 35 are arranged horizontally and are sleeved on the cam shaft 36 in sequence from the top and face each cam portion 36a; a hole is provided on the rear side of the movable pressing plate 35 and is sleeved on the cam portion 36a, and both sides are inserted into the horizontal guide grooves 34a on the pressing plate guide block 34, and the front side extends from the pressing plate extension opening 34b to form a strip-shaped extrusion portion 35a;

[0061] The fixed cut-off plate 37 is fixed on the pressure plate guide block 34 and is located at the middle position of the cam shaft 36, dividing the movable pressure plate 35 into two parts, the upper and lower parts. The cam portion 36a can push the upper and lower parts of the movable pressure plate 35 to push the siphon tube 31 forward from top to bottom in sequence during the directional rotation of the cam shaft 36;

[0062] The front mounting plate 38 is a frame structure and is buckled on the front side of the mounting opening 32a. The cut-off door 39 and the lock rod 41 are respectively arranged on the left and right sides of the mounting plate 38. The cut-off door 39 is connected to the front mounting plate 38 through a torsion spring and a door shaft.

[0063] The front mounting plate 38 is provided with a vertical mounting groove 38a for mounting the locking rod 41, and the middle of the upper and lower sides thereof is provided with a siphon tube clamping groove 38b for inserting the siphon tube 31;

[0064] The lock rod 41 can be inserted into the vertical installation groove 38a movably up and down, a lock hook 41a is provided on the front side of the lock rod 41, and a lock hole 38c for the lock hook 41a to pass through is provided on the front side of the vertical installation groove 38a. One end of the lock rod 41 extends out of the vertical installation groove 38a to form an operating portion 41b, and a lock buckle 39a that can be engaged with the lock hook 41a is provided on the inner side of the cut-off door 39. A compression spring is also sleeved on the lock rod 41, and one end of the compression spring abuts against the front installation plate 38 and pushes the lock rod 41 to have a unidirectional movement tendency so that the lock hook 41a is connected with the lock buckle 39a.

[0065] The movable cut-off plate 390 is installed on the inner side of the cut-off door 39 through a rotating shaft, and the connection side is close to the connection side of the cut-off door 39 and the front mounting plate 38. A spring piece 50 is provided between the front side of the movable cut-off plate 390 and the rear side of the cut-off door 39. When the cut-off door 39 is closed, the spring piece 50 can push the movable cut-off plate 390 to swing forward and press against the fixed cut-off plate 37 to cut off the siphon tube 31.

[0066] The electric push rod 42 is fixed on the front mounting plate 38 and is located on the side where the locking rod 41 is located. The electric push rod 42 can push the movable cutting plate 390 to swing backward to make it disengage from the fixed cutting plate 37 and stop cutting.

[0067] Multiple siphon drive devices 30 and weighing boxes 40 are arranged in a linear array along the length direction of the water bath 20, and correspond one to one; frozen plasma bags are evenly arranged in the drain basket 21 along the length direction of the water bath 20, and transfer bags are respectively provided in the weighing boxes 40, and the transfer bags are connected to the frozen plasma bags through siphon tubes 31.

[0068] It also includes a water circulation component 5, which includes a constant temperature water tank, a heat exchanger, a condenser, a compressor, a water inlet pump, and a circulating pump; the compressor is connected to the condenser and the cooling side of the heat exchanger through a refrigerant pipeline to form a refrigeration circuit; the water inlet hole is connected to the constant temperature water tank through a water pipe and a water inlet pump, and the water outlet hole is connected to the constant temperature water tank through a water pipe; the hot side of the heat exchanger is connected to the constant temperature water tank through a circulating pump and pipelines on both sides.

[0069] In the present application, return water is collected by a water outlet box connected to the water outlet hole, and uniform water outlet is achieved by connecting multiple water outlet pipes 60 from the water outlet box. At the same time, a water inlet box is arranged at the bottom of the water bath 20. The top of the water inlet box, i.e., the bottom of the water tank, is densely covered with water inlet holes. The water inlet box is connected to the water inlet pump through multiple water inlet pipes. The constant temperature effect in the water bath is improved by uniform water inlet and outlet.

[0070] The method of using the cold precipitation coagulation factor preparation instrument based on the siphon method is as follows:

[0071] Step 1, preparing low-temperature water for cold precipitation through a water circulation component or other means, and introducing the low-temperature water into a water bath, and starting the water circulation in the water bath after the water temperature stabilizes;

[0072] Step 2, after placing the double bag into a weighing box, weigh and record the total weight; take out the plasma bag and the transfer bag from the weighing box, place the frozen plasma bag in a water tank, place the transfer bag in the weighing box, and clamp the siphon tube in the siphon tube clamping groove (38b) on the front mounting plate (38), so that the middle part of the siphon tube passes through each strip extrusion part (35a) and the fixed cut-off plate (37);

[0073] Step 3, close the cut-off door (39), lock the cut-off door (39) by means of the lock hook (41a) on the lock rod (41) and the lock buckle (39a) on the cut-off door (39), and then open the stop clamp between the two blood bags;

[0074] Step 4, waiting for the frozen plasma bag to initially melt, then driving the camshaft (36) to rotate through the camshaft drive motor (330) or the rotary drive element, driving each strip-shaped extrusion portion (35a) to squeeze the adsorption tube from top to bottom and release it from top to bottom, using low-pressure adsorption to make the melted components in the frozen plasma bag flow from top to bottom along the adsorption tube, repeating until the components reach the transfer bag, using the liquid's own weight and height difference to produce a siphon effect, and turning off the camshaft drive motor (330) or the rotary drive element;

[0075] Step 5, start the electric push rod (42) to push the movable cut-off plate (390) to swing backward to separate it from the fixed cut-off plate (37);

[0076] Step 6: Use a weighing device to monitor the mass of the components in the empty bag to determine whether enough cold precipitated coagulation factors have been separated. When enough cold precipitated coagulation factors have been collected, close the electric push rod (42), release the movable cut-off plate (390), and the spring (50) pushes the movable cut-off plate (390) to swing forward and press against the fixed cut-off plate (37) to cut off the siphon tube (31);

[0077] Step 7: Lock the plasma bag or the plasma bag catheter with the tube clamp provided with the plasma bag; take out the plasma bag with cold precipitation formed in the water bath and the plasma transfer bag with cold precipitation formed in the weighing box, and disconnect the heat seal.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A cryoprecipitated coagulation factor preparation instrument based on the siphon method, characterized in that: include: A frame (1), a water bath assembly (2), a siphon assembly (3), and a measuring assembly (4); The water bath assembly (2) comprises a water bath tank (20) arranged on the inner frame (10), the water bath tank (20) has a water outlet hole on its side wall and a water inlet hole at its bottom; The measuring assembly (4) comprises a plurality of weighing boxes (40) arranged at the lower front side of the water bath (20) and a weighing device located at the bottom of the weighing boxes (40); The siphon assembly (3) comprises a siphon driving device (30) arranged at the front side of the water bath (20), and a siphon tube (31) for connecting the frozen plasma bag and the transfer bag; The siphon driving device (30) comprises: a rear mounting plate (32), a mounting box (33), a pressure plate guide block (34), a movable pressure plate (35), a cam shaft (36), a fixed cut-off plate (37), a front mounting plate (38), a cut-off door (39), a movable cut-off plate (390), a locking rod (41), and an electric push rod (42); The rear mounting plate (32) is fixed on the frame (1), and a mounting opening (32a) is provided on the rear mounting plate (32). The mounting box (33) is located at the rear side of the rear mounting plate, and the front side of the mounting box (33) is open and buckled at the rear side of the mounting opening (32a); Two pressure plate guide blocks (34) are symmetrically spliced ​​to form a boxed structure and fixed in the installation box (33); a plurality of horizontal guide grooves (34a) are provided inside the pressure plate guide block (34); a pressure plate extension opening (34b) is provided on the front side of the horizontal guide groove (34a); and camshaft mounting holes (34c) are provided on the top and bottom of the pressure plate guide block (34); The camshaft (36) is rotatably inserted into the camshaft mounting hole (34c), and a plurality of cam portions (36a) are provided on the camshaft (36); A plurality of movable pressing plates (35) are arranged horizontally and are sleeved on the cam shaft (36) in sequence from the top and face each cam portion (36a); a hole is provided on the rear side of the movable pressing plate (35) and is sleeved on the cam portion (36a), with both sides inserted into the horizontal guide groove (34a), and the front side extends out from the pressing plate extension opening (34b) to form a strip-shaped extrusion portion (35a); The fixed cut-off plate (37) is fixed on the pressure plate guide block (34) and is located at a middle position of the cam shaft (36). The fixed cut-off plate (37) divides the movable pressure plate (35) into two parts, an upper part and a lower part. When the cam shaft (36) rotates in a directional manner, the cam portion (36a) can push the upper and lower movable pressure plates (35) to push the siphon tube (31) forward in sequence from top to bottom; The front mounting plate (38) is a frame structure and is buckled on the front side of the mounting opening (32a). The cut-off door (39) and the lock rod (41) are respectively arranged on the left and right sides of the mounting plate (38). The cut-off door (39) is connected to the front mounting plate (38) through a torsion spring and a door shaft. A vertical mounting groove (38a) for mounting a locking rod (41) is provided on the front mounting plate (38), and a siphon tube clamping groove (38b) for inserting a siphon tube (31) is provided in the middle of the upper and lower sides thereof; The lock rod (41) can be inserted into the vertical installation groove (38a) movably up and down, a lock hook (41a) is provided on the front side of the lock rod (41), and a lock hole (38c) for the lock hook (41a) to pass through is provided on the front side of the vertical installation groove (38a), one end of the lock rod (41) extends out of the vertical installation groove (38a) to form an operating part (41b), and a lock buckle (39a) that can be engaged with the lock hook (41a) is provided on the inner side of the cut-off door (39), and a compression spring is also sleeved on the lock rod (41), one end of the compression spring abuts against one end of the front installation plate (38) to push the lock rod (41) to have a tendency of unidirectional movement so that the lock hook (41a) is connected with the lock buckle (39a); The movable cut-off plate (390) is installed on the inner side of the cut-off door (39) via a rotating shaft, and the connection side is close to the connection side of the cut-off door (39) and the front mounting plate (38). A spring sheet (50) is provided between the front side of the movable cut-off plate (390) and the rear side of the cut-off door (39). When the cut-off door (39) is closed, the spring sheet (50) can push the movable cut-off plate (390) to swing forward and press against the fixed cut-off plate (37) so that the siphon tube (31) is cut off. The electric push rod (42) is fixed on the front mounting plate (38) and is located on the side where the locking rod (41) is located. The electric push rod (42) can push the movable cut-off plate (390) to swing backwards so that it is separated from the fixed cut-off plate (37) and stops cutting.

2. The cold precipitation coagulation factor preparation instrument based on the siphon method according to claim 1, characterized in that: The siphon driving device (30) further comprises: a camshaft driving motor (330) arranged at the rear side of the mounting box (33), and a transmission gear set (331) for connecting the output shaft of the camshaft driving motor (330) and the camshaft (36).

3. The cold precipitation coagulation factor preparation instrument based on the siphon method according to claim 2, characterized in that: It also includes a water circulation component (5), which includes a constant temperature water tank, a heat exchanger, a condenser, a compressor, a water inlet pump, and a circulation pump; the compressor is connected to the condenser and the refrigeration side of the heat exchanger through a refrigerant pipeline to form a refrigeration circuit; the water inlet hole is connected to the constant temperature water tank through a water pipe and the water inlet pump, and the water outlet hole is connected to the constant temperature water tank through a water pipe; the hot side of the heat exchanger is connected to the constant temperature water tank through a circulation pump and pipelines on both sides.

4. The cold precipitation coagulation factor preparation instrument based on the siphon method according to claim 3, characterized in that: The inner frame (10) includes a first mounting surface (10a) and a second mounting surface (10b) formed by a plurality of horizontal support rods, wherein the second mounting surface (10b) is located below the front side of the first mounting surface (10a); the bottom support rods of the first mounting surface (10a) form a first mounting cavity (10c), and the bottom support rods of the second mounting surface (10b) form a second mounting cavity (10d); the outer panel frame (11) is composed of panels located at the front, back, left and right sides and a top panel, and the top panel is provided with a mounting window (10e) facing the first mounting surface (10a) and the second mounting surface (10b).

5. The cold precipitation coagulation factor preparation instrument based on the siphon method according to claim 4, characterized in that: The water bath (20) is a long strip trough structure, a water outlet box (22) is provided at the rear side thereof, a drain basket (21) is provided inside the water inlet box (22), the front wall surface of the water inlet box (22) is shared with the rear wall surface of the water bath (20), and a plurality of water outlet holes (22a) are evenly arranged on the wall surface, and water inlet holes (20a) are evenly arranged at the bottom of the water bath (20); the constant temperature water tank is located in the second installation cavity (10d), and the heat exchanger, condenser, compressor, water inlet pump, and circulation pump are located in the first installation cavity (10c).

6. The cold precipitation coagulation factor preparation instrument based on the siphon method according to claim 5, characterized in that: A plurality of siphon drive devices (30) and weighing boxes (40) are arranged in a linear array along the length direction of the water bath (20) and correspond one to one; frozen plasma bags are evenly arranged in the drain basket (21) along the length direction of the water bath (20), and transfer bags are respectively arranged in the weighing boxes (40), and the transfer bags are connected to the frozen plasma bags through the siphon tubes (31).

7. The cold precipitation coagulation factor preparation instrument based on the siphon method according to claim 1, characterized in that: The frame (1) is used to support the whole equipment, and includes an inner frame (10) formed by connecting a plurality of support rods and an outer plate frame (11) located outside the inner frame (10); usually, the inner frame (10) is formed by light aluminum alloy pre-frames spliced ​​by bolts and connectors, and the outer plate frame (11) is formed by sheet metal stamping welding or plastic plate splicing.

8. A method for using the siphon-based cryoprecipitation coagulation factor preparation apparatus according to claims 1 to 7, characterized in that: The steps include: Step 1, preparing low-temperature water for cold precipitation through a water circulation component or other means, and introducing the low-temperature water into a water bath, and starting the water circulation in the water bath after the water temperature stabilizes; Step 2, after placing the double bag into a weighing box, weigh and record the total weight; take out the plasma bag and the transfer bag from the weighing box, place the frozen plasma bag in a water tank, place the transfer bag in the weighing box, and clamp the siphon tube in the siphon tube clamping groove (38b) on the front mounting plate (38), so that the middle part of the siphon tube passes through each strip extrusion part (35a) and the fixed cut-off plate (37); Step 3, close the cut-off door (39), lock the cut-off door (39) by means of the lock hook (41a) on the lock rod (41) and the lock buckle (39a) on the cut-off door (39), and then open the stop clamp between the two blood bags; Step 4, waiting for the frozen plasma bag to initially melt, then driving the camshaft (36) to rotate through the camshaft drive motor (330) or the rotary drive element, driving each strip-shaped extrusion portion (35a) to squeeze the adsorption tube from top to bottom and release it from top to bottom, using low-pressure adsorption to make the melted components in the frozen plasma bag flow from top to bottom along the adsorption tube, repeating until the components reach the transfer bag, using the liquid's own weight and height difference to produce a siphon effect, and turning off the camshaft drive motor (330) or the rotary drive element; Step 5, start the electric push rod (42) to push the movable cut-off plate (390) to swing backward to separate it from the fixed cut-off plate (37); Step 6: Use a weighing device to monitor the mass of the components in the empty bag to determine whether enough cold precipitated coagulation factors have been separated. When enough cold precipitated coagulation factors have been collected, close the electric push rod (42), release the movable cut-off plate (390), and the spring (50) pushes the movable cut-off plate (390) to swing forward and press against the fixed cut-off plate (37) to cut off the siphon tube (31); Step 7: Lock the plasma bag or the plasma bag catheter with the tube clamp provided with the plasma bag; take out the plasma bag with cold precipitation formed in the water bath and the plasma transfer bag with cold precipitation formed in the weighing box, and disconnect the heat seal.

9. The method for using the cold precipitation coagulation factor preparation instrument based on the siphon method according to claim 8, characterized in that: The temperature of the low temperature water is kept constant within the range of 4°C±2°C.

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