Fluorescence in-situ hybridization instrument probe bin temperature adjusting system and control method
By designing a fluorescent in-situ hybridizer probe chamber temperature control system including a condensation box, a temperature regulation device, a humidity regulation assembly and a control host, the problem of poor temperature and humidity adjustment in the probe chamber in the prior art is solved, and the rapid and accurate adjustment of the temperature and humidity in the probe chamber is achieved, and the accuracy and reliability of the experiment are improved.
Patent Information
- Application Number
- CN202510270827.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-07
- Publication Date
- 2025-06-06
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing fluorescence in-situ hybridizer probe chambers perform poorly in temperature and humidity adjustment, making it difficult to respond quickly to the temperature changes required during the experiment, resulting in inefficient experiments and reduced probe effectiveness.
A fluorescent in-situ hybridizer probe chamber temperature control system including a condensation box, a temperature regulating device, a humidity regulating assembly and a control host is designed to achieve rapid and accurate temperature and humidity adjustment through the circulating flow of the transmission medium and the use of a humidifier.
The rapid and precise adjustment of temperature and humidity in the probe chamber is achieved, ensuring the stability and effectiveness of the probe, and avoiding experimental errors and reduced probe effectiveness caused by unstable environmental conditions.
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Figure CN120098783A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of biological experiment reagent storage, and in particular to a temperature control system and control method for a probe chamber of a fluorescence in situ hybridization instrument. Background Art
[0002] Fully automatic fluorescence in situ hybridization is widely used in molecular biology research and clinical diagnosis. It achieves highly specific detection of specific nucleic acid sequences in samples through hybridization reaction between specific DNA or RNA probes and target sequences. With the advancement of science and technology, this type of instrument has played an important role in improving experimental accuracy and shortening experimental cycles, greatly promoting the development of the biomedical field.
[0003] At present, the performance of the probe chamber in the prior art in terms of temperature and humidity regulation is still unsatisfactory. Especially when storing open probe tubes, it is often necessary to quickly adjust the temperature and humidity to meet the actual experimental needs. Existing equipment often has difficulty in quickly adapting to the temperature changes required during the experiment, resulting in low experimental efficiency. For example, when a probe needs to be added, the equipment cannot quickly restore the probe to room temperature; and in other experimental steps, the probe cannot be lowered to the storage temperature and humidity in time. This delay not only affects the accuracy of the experimental results, but may also cause the effectiveness of the probe to decrease, thereby affecting the quality and reliability of the entire experiment.
[0004] Therefore, there is an urgent need for a new probe chamber that can accurately control temperature and humidity in a short period of time to meet the high requirements of modern biological experiments. Summary of the invention
[0005] In order to achieve rapid and efficient adjustment of the temperature and humidity in a probe chamber to improve the accuracy and reliability of biological experiments, the present application provides a probe chamber temperature adjustment system and control method for a fluorescence in situ hybridization instrument.
[0006] The present application provides a fluorescence in situ hybridization instrument probe chamber temperature control system adopts the following technical solution: A probe chamber temperature control system for a fluorescence in situ hybridization instrument, comprising: A condensation box, wherein a probe chamber for accommodating a plurality of probe tubes is provided in the condensation box; A temperature control device, the temperature control device comprising a temperature control component and a temperature detector, the temperature control component can input a transmission medium for cooling or heating the probe compartment into the probe compartment to maintain the temperature in the probe compartment, and the temperature detector is used to detect the temperature in the probe compartment; A humidity control component, the humidity control component includes a humidifier and a humidity detector, the humidifier is arranged in the probe compartment to increase the humidity in the probe compartment, and the humidity detector is used to detect the humidity in the probe compartment; The control host is electrically connected to the temperature control device and the humidity control component. The temperature and humidity in the probe chamber can be set through the control host. The temperature control device and the humidity control component can automatically adjust the temperature and humidity in the probe chamber according to the setting parameters of the control host.
[0007] By adopting the above technical solution, the probe compartment temperature control system of the fluorescence in situ hybridization instrument can achieve fast and accurate temperature and humidity adjustment, ensure the stability and effectiveness of the probe during storage, and avoid experimental errors and decreased probe effectiveness caused by unstable environmental conditions. Specifically, the probe compartment can accommodate multiple probe tubes at a time, ensuring the safe storage of the probes; the temperature control component can quickly respond to temperature change requirements through the circulation of the heat transfer medium, avoiding experimental errors caused by temperature fluctuations, and the temperature detector monitors the temperature changes in the probe compartment in real time to ensure accurate temperature control; the humidifier can increase the humidity of the probe compartment when necessary to prevent the probe from drying and inactivating, and the humidity detector monitors the humidity level in the probe compartment in real time to ensure accurate humidity management; the user can set the target temperature and humidity of the probe compartment through the control host, and the temperature control device and humidity control component will automatically adjust according to these set values, improving the system's automation and ease of operation.
[0008] Optionally, the temperature control component includes a heating refrigerator and a heat exchanger. The control host can control the heating refrigerator to heat or cool the transmission medium according to the external ambient temperature and the temperature set by itself, and transport the transmission medium to the heat exchanger. The heat exchanger is circulated with the probe chamber, and the heat exchanger can use the heat or cold of the transmission medium to heat or cool the air in the probe chamber.
[0009] By adopting the above technical solution, the temperature control component can quickly respond to temperature changes, effectively improving the speed and accuracy of temperature adjustment in the probe chamber. Specifically, the control host can accurately control the heating or cooling process of the transmission medium by the heating and cooling device according to the external ambient temperature and the set temperature parameters, and transport it to the heat exchanger; the heat exchanger is connected to the probe chamber in a loop, and the heat or cold of the transmission medium is used to efficiently adjust the air temperature in the probe chamber, ensuring that the temperature in the probe chamber quickly reaches and is maintained within the required range, significantly improving the accuracy and reliability of biological experiments.
[0010] Optionally, an automatic bin cover assembly is also included, which includes a bin cover and a driving member. The bin cover is hingedly connected to the condensation box to cover the opening of the probe bin. The driving member is electrically connected to the control host, and the control host can control the driving member to drive the bin cover to flip to open or close the probe bin.
[0011] By adopting the above technical solution, the automatic chamber cover assembly can automatically open or close the opening of the probe chamber under the command of the control host. This not only improves the convenience of operation, but also reduces the error and contamination risk caused by manual operation, ensures the stability and safety of the environment in the probe chamber, and further improves the accuracy and reliability of biological experiments.
[0012] Optionally, the automatic bin cover assembly further includes a magnetic coupler and a torque sensor, the magnetic coupler being electrically connected to the torque sensor, the bin cover being connected to a driving member via the magnetic coupler, the torque sensor being used to measure the torque of a rotating shaft hinged between the bin cover and the condensation box, and when the torque sensor measures that the torque of the rotating shaft hinged between the bin cover and the condensation box exceeds a preset value, the magnetic coupler disconnects the bin cover from the driving member.
[0013] By adopting the above technical solution, the combined use of the magnetic coupler and the torque sensor can effectively prevent the bin cover from damaging the equipment due to excessive resistance during the automatic opening or closing process. Specifically, when the torque sensor detects that the torque of the hinged shaft of the bin cover and the condensation box exceeds the preset value, the magnetic coupler will immediately disconnect the connection between the bin cover and the drive member, avoiding mechanical damage caused by excessive external force; this design not only improves the safety of the system, but also extends the service life of the equipment; at the same time, the mechanism ensures that the bin cover can stop moving in time when encountering an obstacle, further improving the safety and reliability of the operation; and when the power is off, the bin cover can be easily opened manually to transfer the probe tube.
[0014] Optionally, it also includes a support plate arranged in the probe chamber, the support plate divides the probe chamber into an upper chamber and a lower chamber; the humidity control component is arranged in the upper chamber, the lower chamber is filled with water, and the lower chamber is also provided with a heat exchange coil, the heat exchange coil is circulated and connected with the heat exchanger to heat or cool the water in the lower chamber through the heat exchanger; a plurality of accommodating holes for accommodating probe test tubes are opened on the support plate; when the probe test tube is inserted in the accommodating hole, the tube mouth end of the probe test tube is located in the upper chamber, and the end of the probe test tube away from the tube mouth is located in the lower chamber in contact with the water.
[0015] By adopting the above technical solution, the humidity control component can efficiently increase the humidity of the upper chamber, ensure the appropriate humidity environment required during the experiment, and reduce the volatilization of the probe; the heat exchange coil and the heat exchanger are circulated and connected so that the water in the lower chamber can be quickly heated or cooled, thereby achieving uniform and efficient temperature regulation of the probe test tube through a water bath; this method is faster and more stable than traditional air heating, avoiding local overheating or overcooling caused by uneven air, thereby improving the accuracy and reliability of the experimental results; in addition, since the upper chamber is mainly responsible for humidity control and the lower chamber is mainly responsible for temperature control, the two chambers do not interfere with each other, making the temperature and humidity control effect of the entire system more accurate and efficient.
[0016] Optionally, the support plate is located on one side of the lower chamber and is provided with a plurality of first flip plates for covering the accommodating holes, and the plurality of first flip plates correspond one-to-one to the plurality of accommodating holes; when the probe test tube is inserted into the accommodating hole, the first flip plate automatically opens the accommodating hole; when the probe test tube is taken out of the accommodating hole, the first flip plate automatically covers the accommodating hole.
[0017] By adopting the above technical solution, the first flip plate can automatically respond to the insertion and removal of the probe test tube; when the probe test tube is inserted into the receiving hole, the first flip plate will automatically open to allow the probe test tube to enter smoothly; and when the probe test tube is taken out of the receiving hole, the first flip plate will immediately automatically cover the receiving hole, effectively preventing dust and impurities in the external environment from entering the probe chamber, ensuring the cleanliness of the probe test tube and the accuracy of the experiment; at the same time, this design can also reduce the gas exchange inside and outside the probe chamber, help maintain a constant temperature and humidity in the probe chamber, and further improve the stability and reliability of the system.
[0018] Optionally, a water-absorbing sponge is provided at the opening of the accommodating hole at one end of the upper chamber, and the water-absorbing sponge is slidably abutted against the outer wall of the probe test tube to absorb moisture from the outer wall of the probe test tube when the probe test tube is taken out.
[0019] By adopting the above technical solution, when taking the probe test tube, the absorbent sponge can effectively absorb the moisture on the outer wall of the probe test tube to prevent moisture from dripping and polluting the environment in the upper chamber; at the same time, the moisture absorbed by the absorbent sponge will be gradually released into the upper chamber, which helps to maintain and increase the humidity of the upper chamber, ensuring that the humidity in the probe chamber is always within an appropriate range, further improving the stability and reliability of the experiment; in addition, because the absorbent sponge is in close sliding contact with the outer wall of the probe test tube, it can also reduce the possibility of external contaminants entering the lower chamber when the probe test tube is placed in the receiving hole.
[0020] Optionally, the compartment cover is provided with a plurality of taking holes, and the plurality of taking holes correspond one-to-one to the plurality of accommodating holes, and the reagents in the probe tube can be directly sucked out from the outside of the compartment cover or the probe tube can be taken out through the taking holes; a second flip plate is provided at one end of the taking hole located in the upper chamber; the second flip plate automatically closes the taking hole in a free state; the second flip plate can be flipped into the upper chamber to open the taking hole under the action of external force.
[0021] By adopting the above technical solution, the multiple taking holes on the bin cover can conveniently directly absorb the reagents in the probe test tubes at the corresponding positions from the outside or take out the probe test tubes without frequently opening the entire bin cover, thereby reducing the humidity loss caused by frequent opening of the cover; the second flip plate automatically closes the taking holes in a natural state, effectively preventing moisture in the environment from entering the probe bin, thereby reducing the humidity loss caused by directly opening the cover; when operation is required, the second flip plate can be easily opened by applying external force, making the operation more convenient and efficient; this design not only simplifies the experimental process, but also ensures the stability of the humidity in the probe bin, thereby improving the reliability and accuracy of the experimental results.
[0022] Optionally, a plurality of separation sleeves are provided between the taking hole and the accommodating hole, and the plurality of separation sleeves correspond to the accommodating holes one by one, one end of the separation sleeve is sealedly connected to the bin cover and connected to the accommodating hole, and the other end is sealedly abutted against the support plate; the separation sleeve is provided with a connecting hole connecting the inner cavity of the separation sleeve and the upper chamber; a rotating sleeve is threadedly connected to the taking hole, through which the reagent in the probe test tube can be directly sucked from the outside of the bin cover or the probe test tube can be taken out; rotating the rotating sleeve to move into the upper chamber can make the rotating sleeve abut the second flip plate to flip into the upper chamber to open the taking hole, and make the second flip plate cover the connecting hole to close the connecting hole.
[0023] By adopting the above technical solution, each probe tube has an independent moisturizing chamber at one end of the upper chamber. Specifically, the design of the separation sleeve ensures that a closed small environment is formed between each receiving hole and the corresponding access hole, which reduces the cross-contamination between different probe tubes to a certain extent. At the same time, the unique structural design of the rotating sleeve allows the operator to rotate the rotating sleeve to push open the second flip plate and smoothly open the access hole to facilitate the absorption or replacement of the reagent in the probe tube; in addition, when the access hole is opened, the second flip plate will automatically cover the connecting hole after being pushed open, further reducing the loss of humidity in the upper chamber, ensuring the stability of the humidity in the probe compartment, and improving the reliability and accuracy of the experiment.
[0024] Optionally, a method for controlling a temperature control system of a probe chamber of a fluorescence in situ hybridization instrument comprises the following steps: S1: Setting the target temperature and target humidity, and setting the target temperature and target humidity of the probe chamber (11) by controlling the host (4); S2: Detecting the current temperature and humidity, and detecting the current temperature and humidity in the probe chamber (11) in real time through a temperature detector (22) and a humidity detector (32); S3: adjusting the temperature. If the current temperature is higher than the target temperature, the cooling function in the temperature adjustment component (21) is activated to reduce the temperature in the probe chamber (11); if the current temperature is lower than the target temperature, the heating function in the temperature adjustment component (21) is activated to increase the temperature in the probe chamber (11); S4: adjusting the humidity. If the current humidity is lower than the target humidity, the humidification function in the humidity control component (3) is activated to increase the humidity in the probe chamber (11); if the current humidity is higher than the target humidity, the humidification function is stopped to allow natural evaporation and dehumidification; S5: Monitor and maintain a stable state, and continuously monitor the temperature and humidity in the probe chamber (11) to ensure that they reach and remain within a target range.
[0025] By adopting the above technical solution, the temperature and humidity in the probe chamber can be precisely controlled, significantly improving the reliability and accuracy of biological experiments; this method can quickly respond to environmental changes, adjust the temperature and humidity in the probe chamber in time, and avoid experimental errors caused by temperature and humidity fluctuations.
[0026] In summary, this application includes the following beneficial technical effects: 1. The probe compartment temperature control system of the fluorescence in situ hybridization instrument can achieve fast and accurate temperature and humidity adjustment, ensure the stability and effectiveness of the probe during storage, and avoid experimental errors and reduced probe effectiveness caused by unstable environmental conditions. Specifically, the probe compartment can accommodate multiple probe tubes at a time, ensuring the safe storage of the probes; the temperature control component can quickly respond to temperature changes through the circulation of the heat transfer medium, avoiding experimental errors caused by temperature fluctuations, and the temperature detector monitors the temperature changes in the probe compartment in real time to ensure precise temperature control; the humidifier can increase the humidity in the probe compartment when necessary to prevent the probe from drying out and inactivating, and the humidity detector monitors the humidity level in the probe compartment in real time to ensure precise humidity management; the user can set the target temperature and humidity of the probe compartment through the control host, and the temperature control device and humidity control component will automatically adjust according to these set values, improving the system's automation and ease of operation; 2. The combined use of the magnetic coupler and the torque sensor can effectively prevent the bin cover from damaging the equipment due to excessive resistance during the automatic opening or closing process. Specifically, when the torque sensor detects that the torque of the hinged shaft between the bin cover and the condensation box exceeds the preset value, the magnetic coupler will immediately disconnect the bin cover from the drive component, avoiding mechanical damage caused by excessive external force. This design not only improves the safety of the system, but also extends the service life of the equipment. At the same time, the mechanism ensures that the bin cover can stop moving in time when encountering an obstacle, further improving the safety and reliability of the operation. In addition, the bin cover can be easily opened manually to transfer the probe tube when the power is off. 3. Each probe tube has an independent moisturizing chamber at one end of the upper chamber. Specifically, the design of the separation sleeve ensures that a closed small environment is formed between each receiving hole and the corresponding access hole, which reduces the cross-contamination between different probe tubes to a certain extent. At the same time, the unique structural design of the rotating sleeve allows the operator to rotate the rotating sleeve to push open the second flip plate and smoothly open the access hole to facilitate the absorption or replacement of the reagent in the probe tube; in addition, when the access hole is opened, the second flip plate will automatically cover the connecting hole after being pushed open, further reducing the loss of humidity in the upper chamber, ensuring the stability of the humidity in the probe compartment, and improving the reliability and accuracy of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 It is a schematic diagram of the overall structure of Example 1 of the present application.
[0028] Figure 2 It is a schematic diagram of the internal structure of Example 1 of the present application.
[0029] Figure 3 It is a schematic diagram of the internal structure of Example 2 of the present application.
[0030] Figure 4 yes Figure 3 A partial enlarged view of part A.
[0031] Explanation of the reference numerals: 100, probe tube; 1, condensation box; 11, probe chamber; 111, upper chamber; 112, lower chamber; 2, temperature control device; 21, temperature control assembly; 211, heating refrigerator; 212, heat exchanger; 22, temperature detector; 3, humidity control assembly; 31, humidifier; 32, humidity detector; 4, control host; 5, automatic chamber cover assembly; 51, chamber cover; 511, taking hole; 52, driving member; 53, magnetic coupler; 54, torque sensor; 6, support plate; 61, receiving hole; 7, heat exchange coil; 8, first flip plate; 9, water-absorbing sponge; 10, second flip plate; 20, separation sleeve; 30, connecting hole; 40, rotating sleeve. DETAILED DESCRIPTION
[0032] The following combination Figure 1-Figure 4 This application is described in further detail.
[0033] The embodiments of the present application disclose a temperature control system and control method for a probe chamber of a fluorescence in situ hybridization instrument.
[0034] Example 1 Reference Figure 1 and Figure 2 In this embodiment, the probe chamber temperature control system of the fluorescence in situ hybridization instrument includes a condensation box 1, a temperature control device 2, a humidity control component 3, a control host 4 and an automatic chamber cover component 5. Among them, a probe chamber 11 for accommodating multiple probe tubes 100 is opened in the condensation box 1; multiple probe tubes 100 are arrayed and installed in the probe chamber 11. The condensation box 1 is a rectangular box with an upper opening, and is made of high-strength plastic material. The wall of the condensation box 1 is provided with a heat-insulating interlayer to enhance the heat-insulating performance of the condensation box 1. In other embodiments, the condensation box 1 can also be other shapes; the material of the condensation box 1 can also be aluminum alloy or stainless steel.
[0035] Reference Figure 1 and Figure 2 In this embodiment, the temperature control device 2 includes a temperature control component 21 and a temperature detector 22. The temperature control component 21 can input a transmission medium for cooling or heating the probe compartment 11 into the probe compartment 11 to maintain the temperature in the probe compartment 11. The temperature detector 22 is arranged in the probe compartment 11 to detect the temperature in the probe compartment 11; the humidity control component 3 includes a humidifier 31 and a humidity detector 32. The humidifier 31 is arranged in the probe compartment 11 to increase the humidity in the probe compartment 11, and the humidity detector 32 is arranged in the probe compartment 11 to detect the humidity in the probe compartment 11; the control host 4 is electrically connected to the temperature control device 2 and the humidity control component 3. The temperature and humidity in the probe compartment 11 can be set by the control host 4. The temperature control device 2 and the humidity control component 3 can automatically adjust the temperature and humidity in the probe compartment 11 according to the setting parameters of the control host 4.
[0036] Reference Figure 1 and Figure 2 In this embodiment, the temperature control component 21 includes a heating refrigerator 211 and a heat exchanger 212; the control host 4 can control the heating refrigerator 211 to heat or cool the transmission medium according to the external ambient temperature and the temperature set by itself, and transport the transmission medium to the heat exchanger 212. The heat exchanger 212 is circulated and connected with the probe chamber 11. The heat exchanger 212 uses the heat or cold of the transmission medium to circulate and heat or cool the air in the probe chamber 11; the heating refrigerator 211 can use common heating and cooling devices such as semiconductor heating refrigerators or small compressors, and the heat exchanger 212 can be a copper tube or aluminum fin heat exchanger 212.
[0037] The humidifier 31 in the humidity control component 3 can be an ultrasonic humidifier or a spray humidifier, and the humidity detector 32 can be a resistive humidity sensor or a capacitive humidity sensor; the ultrasonic humidifier generates tiny water droplets through high-frequency vibration, which are evenly distributed in the probe compartment 11 to increase the humidity; the resistive humidity sensor measures the moisture content in the air and feeds back humidity information to the control host 4.
[0038] The control host 4 can be an embedded microcomputer with a touch screen interface, which is convenient for users to set temperature and humidity parameters; the control host 4 can also integrate a data recording function to record the temperature and humidity changes in the probe chamber 11 in real time, which is convenient for subsequent analysis and tracing. In other embodiments, the heat exchanger 212 can also use a stainless steel coil; the humidifier 31 can also use an evaporative humidifier, and the humidity detector 32 can also use a photoelectric humidity sensor.
[0039] Reference Figure 1 and Figure 2 In this embodiment, the automatic bin cover assembly 5 includes a bin cover 51, a driving member 52, a magnetic coupler 53 and a torque sensor 54. The bin cover 51 is hingedly connected to the condensation box 1 to cover the opening of the probe bin 11. The material of the bin cover 51 is the same as that of the condensation box 1. The bin cover 51 is also provided with a thermal insulation interlayer to improve the thermal insulation performance; the driving member 52 is electrically connected to the control host 4, and the control host 4 can control the driving member 52 to drive the bin cover 51 to flip to open or close the probe bin 11; the driving member 52 is a motor, and the motor drives the bin cover 51 to rotate through gear transmission or belt transmission; for example, the motor can be a stepper motor or a servo motor. The stepper motor is suitable for low-speed and high-precision application scenarios, and the servo motor is suitable for high-speed response requirements; the communication between the driving member 52 and the control host 4 can be achieved through a wireless module or a wired interface to ensure the reliable transmission of the control signal.
[0040] The magnetic coupler 53 is electrically connected to the torque sensor 54, and the bin cover 51 is connected to the driving member 52 through the magnetic coupler 53. The torque sensor 54 is used to measure the torque of the rotating shaft hinged between the bin cover 51 and the condensation box 1. It should be pointed out that the magnetic coupler 53 is an existing mature technology, so it will not be described in detail here; when the torque sensor 54 measures that the torque of the rotating shaft hinged between the bin cover 51 and the condensation box 1 exceeds a preset value, the magnetic coupler 53 will disconnect the bin cover 51 from the driving member 52 to prevent excessive torque from damaging the equipment; this design effectively avoids equipment failures caused by accidental jamming or other abnormal conditions.
[0041] The implementation principle of this embodiment is as follows: the system realizes precise control of the temperature and humidity in the probe chamber 11 through the coordinated work of the temperature control device 2 and the humidity control component 3; the control host 4 automatically adjusts the working state of the heating and cooling device 211 according to the user's set parameters, so that the temperature in the probe chamber 11 quickly reaches the set value; at the same time, the humidifier 31 is turned on or off in time according to the feedback signal of the humidity detector 32 to ensure that the humidity in the probe chamber 11 is maintained within an appropriate range; this method not only improves the experimental efficiency, but also ensures the accuracy and reliability of the experimental results, and is particularly suitable for complex experimental conditions that require frequent opening and closing of the probe chamber 11; the introduction of the automatic chamber cover component 5 makes the opening and closing of the probe chamber 11 more convenient and safe, and the control host 4 automatically controls the action of the drive member 52 according to the needs of the experimental process to realize the rapid opening and closing of the chamber cover 51, significantly improving the operating speed and safety; the design of the magnetic coupler 53 and the torque sensor 54 increases the safety of the system and further improves the user experience and the reliability of the equipment.
[0042] Example 2 Reference Figure 3 and Figure 4 The difference between this embodiment 2 and the above-mentioned embodiment 1 is that: the probe chamber temperature control system of the fluorescence in situ hybridization instrument also includes a support plate 6 arranged in the probe chamber 11, and the support plate 6 divides the probe chamber 11 into an upper chamber 111 and a lower chamber 112; the humidity control component 3 is arranged in the upper chamber 111, and the lower chamber 112 is filled with water. The lower chamber 112 is also provided with a heat exchange coil 7, and the heat exchange coil 7 is connected to the heat exchanger 212 through a pipeline circulation to heat or cool the water in the lower chamber 112 through the heat exchanger 212; a plurality of accommodating holes 61 for accommodating probe tubes 100 are opened on the support plate 6; when the probe tube 100 is inserted into the accommodating hole 61, the nozzle end of the probe tube 100 is located in the upper chamber 111, and the end of the probe tube 100 away from the nozzle is located in the lower chamber 112 and in contact with water.
[0043] The support plate 6 can be made of high temperature resistant and corrosion resistant stainless steel material with a smooth surface and easy cleaning; the diameter of the receiving hole 61 on the support plate 6 is slightly larger than the outer diameter of the probe tube 100, so that the probe tube 100 can be easily inserted and removed; the inner wall of the receiving hole 61 is provided with a rubber gasket, which can not only fix the probe tube 100 but also prevent water seepage; the side of the support plate 6 is sealed against the inner wall of the probe chamber 11 to ensure the sealing of the upper chamber 111 and the lower chamber 112; The heat exchange coil 7 is made of brass to ensure that it has good thermal conductivity; the heat exchange coil 7 is completely immersed in the water in the lower chamber 112 and is located at the bottom of the lower chamber 112, so that the heat exchange coil 7 can heat the water in the lower chamber 112 as evenly as possible; the connecting pipe between the heat exchange coil 7 and the heat exchanger 212 should be made of high-pressure and temperature-resistant materials, such as silicone hoses or metal bellows.
[0044] Reference Figure 3 and Figure 4 In this embodiment, a plurality of first flip plates 8 for covering the receiving holes 61 are provided on one side of the support plate 6 located at the lower chamber 112, and the plurality of first flip plates 8 correspond to the plurality of receiving holes 61 one by one; the first flip plate 8 can be made of lightweight plastic or aluminum alloy material, and the surface is coated with an anti-stick coating to prevent the probe tube 100 or reagent from remaining; the first flip plate 8 is connected to the support plate 6 by a spring or a torsion spring, and when the probe tube 100 is inserted into the receiving hole 61, the probe tube 100 pushes the first flip plate 8 to flip and open the receiving hole 61; when the probe tube 100 is taken out of the receiving hole 61, the first flip plate 8 automatically resets to cover the receiving hole 61 under the action of the spring or the torsion spring.
[0045] Preferably, a water-absorbing sponge 9 is provided at the opening of the accommodating hole 61 at one end of the upper chamber 111 , and the water-absorbing sponge 9 slides against the outer wall of the probe test tube 100 to absorb moisture from the outer wall of the probe test tube 100 when the probe test tube 100 is taken out.
[0046] Reference Figure 3 and Figure 4 In this embodiment, a plurality of taking holes 511 are provided on the top of the compartment cover 51. The plurality of taking holes 511 correspond to the plurality of accommodating holes 61 one by one. The diameter of the taking holes 511 is larger than the diameter of the probe tube 100. The reagent in the probe tube 100 can be directly sucked from the outside of the compartment cover 51 or the probe tube 100 can be taken out through the taking holes 511. A second flip plate 10 is provided at one end of the taking hole 511 located in the upper chamber 111. The second flip plate 10 automatically closes the taking hole 511 in a free state. The second flip plate 10 Under the action of external force, the taking hole 511 can be flipped into the upper chamber 111 to open; the second flip plate 10 can also be made of lightweight plastic or aluminum alloy material, and the surface is coated with an anti-stick coating to prevent the probe tube 100 or reagent from remaining; the second flip plate 10 is also connected to the compartment cover 51 through a spring or a torsion spring. When it is necessary to absorb the reagent in the probe tube 100 or take the probe tube 100, the second flip plate 10 can be pushed open to operate, and after the operation is completed, the second flip plate 10 automatically resets and closes the taking hole 511.
[0047] A plurality of separation sleeves are provided between the taking hole 511 and the receiving hole 61, and the plurality of separation sleeves 20 correspond to the receiving hole 61 one by one. One end of the separation sleeve 20 is sealed and connected to the compartment cover 51 and communicates with the receiving hole 61, and the other end is sealed and abuts against the support plate 6 so that the inner cavities of the plurality of separation sleeves 20 form a plurality of independent moisturizing cavities, and each probe test tube 100 corresponds to a moisturizing cavity, which reduces the cross contamination between different probe test tubes 100 to a certain extent; the side wall of the separation sleeve 20 is provided with a connection between the upper chamber 111 and the moisturizing cavity The connecting hole 30; the taking hole 511 is threadedly connected with a rotating sleeve 40, through which the taking hole 511 can be entered to directly absorb the reagent in the probe tube 100 from the outside of the compartment cover 51 or to take out the probe tube 100; when the rotating sleeve 40 is rotated to move toward the interior of the upper chamber 111, the end of the rotating sleeve 40 abuts against the second flip plate 10 to flip it into the upper chamber 111 and then open the taking hole 511. At the same time, the second flip plate 10 covers the connecting hole 30 and closes the connecting hole 30.
[0048] The implementation principle of this embodiment 2 is as follows: the probe chamber 11 is divided into two independent parts, the upper chamber 111 and the lower chamber 112, by the support plate 6; the upper chamber 111 is mainly responsible for humidity control and the lower chamber 112 is mainly responsible for temperature control, and the two chambers do not interfere with each other; the opening of the probe tube 100 is in the upper chamber 111, and the humidity control component 3 is arranged in the upper chamber 111 to ensure the humidity of the reagent to the maximum extent and reduce the volatilization of the reagent; the lower chamber 112 is filled with water and heated or cooled by the heat exchange coil 7, and a part of the probe tube 100 is immersed in water, which can directly absorb or release heat. The advantage of this design is that it can make full use of the characteristics of water's large specific heat capacity and fast heat transfer to quickly adjust the temperature in the probe chamber 11; the design of the separation sleeve 20 enables each probe tube 100 to have an independent small space in the upper chamber 111, which to a certain extent alleviates the different Cross-contamination between probe test tubes 100; the design of the first flip plate 8 can reduce the heat loss of the lower chamber 112 when the probe test tube 100 is not placed in the accommodating hole 61; the design of the taking hole 511 and the second flip plate 10 allows the operator to directly absorb the reagent in the probe test tube 100 or take the probe test tube 100 from the outside of the compartment cover 51 without opening the compartment cover 51, and the second flip plate 10 will close the connecting hole 30 while opening the taking hole 511, so that the overall humidity loss of the upper chamber 111 can be reduced after opening the connecting hole 30; the design of the rotating sleeve 40 allows the switch size of the second flip plate 10 to be freely adjusted and locked; the absorbent sponge 9 can absorb the moisture on the outer wall of the probe test tube 100 when taking the probe test tube 100, and gradually release the moisture into the upper chamber 111, thereby maintaining and increasing the humidity of the upper chamber 111.
[0049] Example 3 Example 3 of the present application also discloses a method for controlling the temperature of a probe chamber of a fluorescence in situ hybridization instrument using the above-mentioned probe chamber temperature control system of the fluorescence in situ hybridization instrument, comprising the following steps: S1: Setting the target temperature and target humidity. The target temperature and target humidity of the probe chamber 11 are set by controlling the host 4 .
[0050] S2: Detect the current temperature and humidity. The current temperature and humidity in the probe chamber 11 are detected in real time by the temperature detector 22 and the humidity detector 32 .
[0051] S3: Adjust the temperature. If the current temperature is higher than the target temperature, start the cooling function in the temperature control component 21 to lower the temperature in the probe chamber 11 ; if the current temperature is lower than the target temperature, start the heating function in the temperature control component 21 to increase the temperature in the probe chamber 11 .
[0052] S4: Adjust the humidity. If the current humidity is lower than the target humidity, start the humidification function in the humidity control component 3 to increase the humidity in the probe chamber 11; if the current humidity is higher than the target humidity, stop the humidification function to allow natural evaporation and dehumidification.
[0053] S5: Monitor and maintain a stable state, and continuously monitor the temperature and humidity in the probe chamber 11 to ensure that they reach and remain within the target range.
[0054] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.
Claims
1. A probe chamber temperature control system for a fluorescence in situ hybridization instrument, characterized in that: include: A condensation box (1), wherein a probe chamber (11) for accommodating a plurality of probe tubes (100) is provided in the condensation box (1); A temperature control device (2), the temperature control device (2) comprising a temperature control component (21) and a temperature detector (22), the temperature control component (21) being capable of inputting a transmission medium for cooling or heating the probe chamber (11) into the probe chamber (11) to maintain the temperature in the probe chamber (11), and the temperature detector (22) being used to detect the temperature in the probe chamber (11); A humidity control component (3), the humidity control component (3) comprising a humidifier (31) and a humidity detector (32), the humidifier (31) being arranged in the probe chamber (11) for increasing the humidity in the probe chamber (11), and the humidity detector (32) being used for detecting the humidity in the probe chamber (11); A control host (4) is electrically connected to the temperature control device (2) and the humidity control component (3), and the temperature and humidity in the probe chamber (11) can be set via the control host (4). The temperature control device (2) and the humidity control component (3) can automatically adjust the temperature and humidity in the probe chamber (11) according to the setting parameters of the control host (4).
2. A probe chamber temperature control system for a fluorescence in situ hybridization instrument according to claim 1, characterized in that: The temperature control component (21) includes a heating refrigerator (211) and a heat exchanger (212). The control host (4) can control the heating refrigerator (211) to heat or cool the transmission medium according to the external environment temperature and the temperature set by itself, and transport the transmission medium to the heat exchanger (212). The heat exchanger (212) is cyclically connected to the probe chamber (11). The heat exchanger (212) can use the heat or cold of the transmission medium to heat or cool the air in the probe chamber (11).
3. A probe chamber temperature control system for a fluorescence in situ hybridization instrument according to claim 1, characterized in that: The invention also comprises an automatic bin cover assembly (5), wherein the automatic bin cover assembly (5) comprises a bin cover (51) and a driving member (52), wherein the bin cover (51) is hingedly connected to the condensation box (1) and is used to cover the opening of the probe bin (11), and the driving member (52) is electrically connected to the control host (4), and the control host (4) can control the driving member (52) to drive the bin cover (51) to flip so as to open or close the probe bin (11).
4. A probe chamber temperature control system for a fluorescence in situ hybridization instrument according to claim 3, characterized in that: The automatic bin cover assembly (5) further comprises a magnetic coupler (53) and a torque sensor (54), wherein the magnetic coupler (53) is electrically connected to the torque sensor (54), and the bin cover (51) is connected to the driving member (52) via the magnetic coupler (53). The torque sensor (54) is used to measure the torque of a rotating shaft hinged between the bin cover (51) and the condensation box (1), and when the torque sensor (54) measures that the torque of the rotating shaft hinged between the bin cover (51) and the condensation box (1) exceeds a preset value, the magnetic coupler (53) disconnects the bin cover (51) from the driving member (52).
5. A probe chamber temperature control system for a fluorescence in situ hybridization instrument according to claim 4, characterized in that: The invention also comprises a support plate (6) arranged in the probe chamber (11), wherein the support plate (6) divides the probe chamber (11) into an upper chamber (111) and a lower chamber (112); the humidity control component (3) is arranged in the upper chamber (111), the lower chamber (112) is filled with water, and a heat exchange coil (7) is also arranged in the lower chamber (112), wherein the heat exchange coil (7) is cyclically connected to the heat exchanger (212) so as to heat or cool the water in the lower chamber (112) through the heat exchanger (212); the support plate (6) is provided with a plurality of accommodating holes (61) for accommodating the probe test tube (100); when the probe test tube (100) is inserted into the accommodating hole (61), the tube mouth end of the probe test tube (100) is located in the upper chamber (111), and the end of the probe test tube (100) away from the tube mouth is located in the lower chamber (112) and contacts the water.
6. A probe chamber temperature control system for a fluorescence in situ hybridization instrument according to claim 5, characterized in that: The support plate (6) is provided with a plurality of first flip plates (8) for covering the receiving holes (61) on one side of the lower chamber (112), and the plurality of first flip plates (8) correspond to the plurality of receiving holes (61) one by one; when the probe test tube (100) is inserted into the receiving hole (61), the first flip plate (8) automatically opens the receiving hole (61); when the probe test tube (100) is taken out of the receiving hole (61), the first flip plate (8) automatically covers the receiving hole (61).
7. A probe chamber temperature control system for a fluorescence in situ hybridization instrument according to claim 6, characterized in that: A water-absorbing sponge (9) is provided at the opening of the receiving hole (61) at one end of the upper chamber (111), and the water-absorbing sponge (9) is in sliding contact with the outer wall of the probe test tube (100) to absorb moisture from the outer wall of the probe test tube (100) when the probe test tube (100) is taken out.
8. The probe chamber temperature control system of a fluorescence in situ hybridization instrument according to claim 5, characterized in that: The compartment cover (51) is provided with a plurality of taking holes (511), and the plurality of taking holes (511) correspond one-to-one to the plurality of accommodating holes (61). The reagent in the probe tube (100) can be directly sucked out from the outside of the compartment cover (51) or the probe tube (100) can be taken out through the taking holes (511); a second flip plate (10) is provided at one end of the taking hole (511) located in the upper chamber (111); the second flip plate (10) automatically closes the taking hole (511) in a free state; and the second flip plate (10) can flip over to open the taking hole (511) in the upper chamber (111) under the action of an external force.
9. A probe chamber temperature control system for a fluorescence in situ hybridization instrument according to claim 8, characterized in that: A plurality of separation sleeves (20) are provided between the taking hole (511) and the receiving hole (61), and the plurality of separation sleeves (20) correspond to the receiving holes (61) one by one. One end of the separation sleeve (20) is sealedly connected to the bin cover (51) and communicates with the receiving hole (61), and the other end is sealedly abutted against the support plate (6); the separation sleeve (20) is provided with a connecting hole (30) connecting the inner cavity of the separation sleeve (20) and the upper chamber (111); the taking hole (511) is internally threadedly connected to a rotating sleeve (40), the taking hole (511) can be entered through the inner cavity of the rotating sleeve (40) to directly absorb the reagent in the probe tube (100) or take the probe tube (100) from the outside of the chamber cover (51); rotating the rotating sleeve (40) to move toward the interior of the upper chamber (111) can make the rotating sleeve (40) abut against the second flip plate (10) to flip into the upper chamber (111) to open the taking hole (511), and make the second flip plate (10) cover the connecting hole (30) to close the connecting hole (30).
10. A method for controlling the temperature of a probe chamber of a fluorescence in situ hybridization instrument using the temperature control system of the probe chamber of a fluorescence in situ hybridization instrument according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: Setting the target temperature and target humidity, and setting the target temperature and target humidity of the probe chamber (11) by controlling the host (4); S2: Detecting the current temperature and humidity, and detecting the current temperature and humidity in the probe chamber (11) in real time through a temperature detector (22) and a humidity detector (32); S3: adjusting the temperature. If the current temperature is higher than the target temperature, the cooling function in the temperature adjustment component (21) is activated to reduce the temperature in the probe chamber (11); if the current temperature is lower than the target temperature, the heating function in the temperature adjustment component (21) is activated to increase the temperature in the probe chamber (11); S4: adjusting the humidity. If the current humidity is lower than the target humidity, the humidification function in the humidity control component (3) is activated to increase the humidity in the probe chamber (11); if the current humidity is higher than the target humidity, the humidification function is stopped to allow natural evaporation and dehumidification; S5: Monitor and maintain a stable state, and continuously monitor the temperature and humidity in the probe chamber (11) to ensure that they reach and remain within a target range.