A kind of suspension pesticide for preventing and treating asparagus stem blight and its preparation method

Abstract

The present application relates to the field of fungal pesticide preparation, in particular to a kind of suspension pesticide for preventing and treating asparagus stem blight and preparation method thereof.The suspension pesticide provided by the present application can effectively prevent and treat asparagus stem blight, and the control effect can reach 94.95%-98.99%.The microorganism combination in the suspension pesticide provided by the present application can prevent pathogenic bacteria from further invading plant body, and improve the disease resistance of plant at the same time;Manganese-zinc maneb can effectively kill fungi and reduce the number of pathogenic bacteria;Myristica and acanthopanax mixed extract can provide nutrition for microorganisms, and also inhibit the growth of pathogenic bacteria.The present application first combines microorganisms, chemical drugs and myristica and acanthopanax mixed extract, and finds that the three have the best effect in a specific ratio, and the synergistic effect between the three is proved by examples.It can be seen that the present application provides a new prevention and treatment method, which can reduce the amount of chemical agent and reduce environmental pollution.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of fungal pesticide formulations, in particular to a suspension pesticide for preventing and treating asparagus stem blight and a preparation method thereof. BACKGROUND

[0002] Asparagus officinalis Linn., also known as asparagus, belongs to Liliaceae and Asparagus. The root of asparagus is 2-3 mm thick; the stem is smooth, and the upper part often bends down in the later stage, with relatively weak branches; the leafy branches of asparagus are in clusters every 3-6, nearly flat cylindrical, slightly sharp, thin, and often slightly curved, 5-30 mm long and 0.3-0.5 mm thick; the scale-like leaf base has a thorny short distance or nearly no distance; the berry is 7-8 mm in diameter, red when ripe, with 2-3 seeds; the flowering period is from May to June, and the fruiting period is from September to October; the tender shoots can be used for food. Asparagus has high nutritional value, can moisten the lungs, relieve cough and expectorant, and has the function of inhibiting tumor growth, etc., and is known as the "King of Vegetables" on the international market. In recent years, with the expansion of asparagus cultivation area, asparagus stem blight disease has become more and more serious year by year, which seriously affects the yield and quality of asparagus.

[0003] Asparagus stem blight is also known as the "cancer" of asparagus, and its main symptoms are concentrated in the stem, lateral branches or leaves, with the characteristics of fast onset, easy transmission and serious loss. Once asparagus is infected, it is easy to spread in a large area, leading to the sudden yellowing and wilting of the whole asparagus plant, and the ineffective output of asparagus products. The main pathogenic bacteria of asparagus stem blight is Phomopsis asparagi. At present, the prevention and treatment of asparagus stem blight mainly uses chemical pesticides such as carbendazim and chlorothalonil, but long-term use of such chemical pesticides can easily lead to drug resistance of the pathogenic bacteria, and cannot effectively prevent and treat asparagus stem blight. Therefore, it is necessary to develop a pesticide formulation with better prevention and treatment effect. SUMMARY

[0004] The purpose of the present application is to provide a suspension pesticide for preventing and treating asparagus stem blight and a preparation method thereof, so as to solve the problems existing in the prior art. The suspension pesticide for preventing and treating asparagus stem blight provided by the present application can effectively prevent and treat asparagus stem blight.

[0005] To achieve the above-mentioned purpose, the present application provides the following solutions:

[0006] The present application provides a suspension pesticide for preventing and treating asparagus stem blight, which comprises effective component 1, effective component 2, effective component 3 and auxiliary materials.

[0007] The effective component 1 is inactivated fermentation liquor of Microdochium nivale and inactivated fermentation liquor of Bacillus pumilus; the effective component 2 is mancozeb; the effective component 3 is mixed extraction liquid of Myristica and Acanthopanax; and the auxiliary material is humectant, dispersant, tackifier, anti-freezing agent and organic solvent.

[0008] Preferably, the mass ratio of the effective component 1, the effective component 2, the effective component 3 and the auxiliary material is 1:(3-5):(4-7):1.

[0009] The mass ratio of the inactivated fermentation liquor of Microdochium nivale and the inactivated fermentation liquor of Bacillus pumilus in the effective component 1 is 1:1.

[0010] The mass ratio of the humectant, the dispersant, the tackifier, the anti-freezing agent and the organic solvent is 3:2:1:1:2.

[0011] Preferably, the preparation method of the mixed extraction liquid of Myristica and Acanthopanax is as follows: Myristica and Acanthopanax are mixed according to a mass ratio of 1:1, and then ethanol extraction is performed to obtain the mixed extraction liquid of Myristica and Acanthopanax.

[0012] Preferably, the humectant is alkylphenol polyoxyethylene ether; the dispersant is sodium tripolyphosphate; the tackifier is polypropylene glycol; the anti-freezing agent is ethylene glycol; and the organic solvent is dipropylene glycol methyl ether.

[0013] The application provides a preparation method of the above-mentioned suspension pesticide, and comprises the following steps:

[0014] The effective component 1, the effective component 2, the effective component 3 and the auxiliary material are mixed to obtain the suspension pesticide.

[0015] The application provides an application of the above-mentioned suspension pesticide in prevention and treatment of asparagus stem blight.

[0016] The application provides a method for preventing and treating asparagus stem blight, and comprises the following steps:

[0017] The above-mentioned suspension pesticide is diluted and then sprayed on the surface of asparagus plants.

[0018] Preferably, the dilution multiple is 500 times.

[0019] Preferably, the use amount of the obtained suspension diluent after dilution is 30 L / acre.

[0020] The application discloses the following technical effects:

[0021] The suspension pesticide provided by the application can effectively prevent and treat asparagus stem blight, and the prevention and treatment efficiency can reach 94.95%-98.99%. The microorganism combination in the suspension pesticide can prevent the pathogenic bacteria from further invading the plant body, and improve the disease resistance of the plant; the mancozeb can effectively kill the fungi and reduce the number of pathogenic bacteria; the mixed extract of myristica and acanthopanax can provide nutrition for the microorganism, and also can inhibit the growth of the pathogenic bacteria. The microorganism, the chemical medicine and the mixed extract of myristica and acanthopanax are combined for the first time in the application, it is found that the three have the best effect in a specific ratio, and the synergistic effect between the three is proved by the examples. Therefore, the application provides a new prevention and treatment method, the use amount of the chemical pesticide can be reduced, and the environmental pollution can be reduced. DETAILED DESCRIPTION

[0022] The various exemplary embodiments of the application will now be described in detail, which should not be considered as limiting the application, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the application.

[0023] It should be understood that the terms described in the application are only for describing the specific embodiments, and are not used to limit the application. In addition, for the numerical range in the application, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intermediate value in the stated range, and any other stated value or intermediate value in the stated range is also included in the application. The upper limit and the lower limit of these smaller ranges can be independently included or excluded from the range.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, the preferred methods and materials are described. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials in connection with which the documents are cited. In case of conflict between the content of the specification and that of any document incorporated herein by reference, the content of the specification prevails.

[0025] Various modifications and changes can be made to the specific embodiments of the application described herein without departing from the scope or spirit of the application. Other embodiments of the application will be apparent to those of ordinary skill in the art from the description and examples presented herein. The description and examples are illustrative of the application and are not intended to limit the scope of the application.

[0026] As used herein, the terms "comprise", "comprising", "include", "including", "have", "having" and the like are open-ended terms that are intended to mean "including but not limited to".

[0027] The strains and components used in the present application are obtained by routine purchase by the skilled in the art, and the methods used in the present application are well known to the skilled in the art, unless otherwise specified.

[0028] Preparation Example

[0029] Streptomyces microflavus SHBCC D60726 is purchased from Wuhan Wallner Biological Technology Co., Ltd., and the item number is WN-BZ42238;

[0030] Preparation of inactivated fermentation broth of Streptomyces microflavus SHBCC D60726: after the activated Streptomyces microflavus SHBCC D60726 is inoculated into 2xYT liquid medium (16 g of protein peptone, 10 g of yeast powder, 5 g of NaCl, and tap water to 1000 mL, natural pH), fermentation culture is carried out to obtain a fermentation broth, and the effective viable cell count in the fermentation broth is 1x10 7 CFU / mL; wherein the inoculation amount is 5%, the fermentation culture time is 72 h, and the temperature is 30℃; then the fermentation broth is inactivated (high-pressure steam inactivation at 115℃ for 15 min) to obtain the inactivated fermentation broth of Streptomyces microflavus SHBCC D60726, which is referred to as the inactivated fermentation broth of Streptomyces microflavus.

[0031] Bacillus pumilus is purchased from Cangzhou Kang Microbial Technology Co., Ltd.;

[0032] Preparation of inactivated fermentation broth of Bacillus pumilus: after the activated Bacillus pumilus is inoculated into PD medium, fermentation culture is carried out to obtain a fermentation broth, and the effective viable cell count in the fermentation broth is 2.1x10 7 CFU / mL; wherein the inoculation amount is 10%, the fermentation culture time is 72 h, the temperature is 35℃, and the rotation speed is 220 rpm; then the fermentation broth is inactivated (high-pressure steam inactivation at 115℃ for 15 min) to obtain the inactivated fermentation broth of Bacillus pumilus.

[0033] Bacillus velezensis is purchased from Gray Algae Biological Technology Co., Ltd., and the item number is HZB136119;

[0034] Preparation of inactivated fermentation broth of Bacillus velezensis: after the activated Bacillus velezensis is inoculated into broth medium, fermentation culture is carried out to obtain a fermentation broth, and the effective viable cell count in the fermentation broth is 2.3x10 7 CFU / mL; wherein the inoculation amount is 10%, the fermentation culture time is 72 h, the temperature is 35℃, and the rotation speed is 220 rpm; then the fermentation broth is inactivated (high-pressure steam inactivation at 115℃ for 15 min) to obtain the inactivated fermentation broth of Bacillus velezensis.

[0035] Bacillus amyloliquefaciens is purchased from Liaoning Huaxing Biological Technology Co., Ltd.;

[0036] Preparation of Bacillus amyloliquefaciens inactivated fermentation broth: the activated Bacillus amyloliquefaciens was inoculated into LB medium for fermentation culture to obtain a fermentation broth, and the effective viable cell number in the fermentation broth was 1.8 x 10 7 CFU / mL; wherein the inoculation amount was 10%, the fermentation culture time was 72 h, the temperature was 35°C, and the rotation speed was 220 rpm; then the fermentation broth was inactivated (high-pressure steam inactivation at 115°C for 15 min) to obtain the Bacillus amyloliquefaciens inactivated fermentation broth.

[0037] Preparation of mixed extract of Myristica and Acanthopanax: Myristica and Acanthopanax were crushed, mixed according to a mass ratio of 1:1 to obtain a mixed powder; the obtained mixed powder was mixed with 75% (volume percentage) ethanol according to a mass-volume ratio of 1 g:12 mL. Extraction was performed at 45°C for 2 h, filtration was performed, ethanol was recovered, and the obtained filtrate was the mixed extract of Myristica and Acanthopanax.

[0038] The above inactivated fermentation broth and the mixed extract of Myristica and Acanthopanax were used in subsequent examples.

[0039] Example 1

[0040] The components of the suspension for preventing and treating asparagus stem blight are as follows:

[0041] Effective component 1 is Bacillus amyloliquefaciens inactivated fermentation broth and Bacillus pumilus inactivated fermentation broth, and the mass ratio of the two is 1:1;

[0042] Effective component 2 is mancozeb;

[0043] Effective component 3 is the mixed extract of Myristica and Acanthopanax;

[0044] The adjuvant is alkylphenol polyoxyethylene ether (wetting agent), sodium tripolyphosphate (dispersant), polypropylene glycol (viscosity increasing agent), ethylene glycol (anti-freezing agent), and dipropylene glycol methyl ether (organic solvent); the mass ratio of alkylphenol polyoxyethylene ether, sodium tripolyphosphate, polypropylene glycol, ethylene glycol, and dipropylene glycol methyl ether is 3:2:1:1:2

[0045] The mass ratio of effective component 1, effective component 2, effective component 3, and the adjuvant is 1:3:5:1.

[0046] The preparation method of the suspension for preventing and treating asparagus stem blight is as follows:

[0047] The effective component 2, the effective component 3, and the adjuvant were mixed, and then the effective component 1 was added to obtain the suspension for preventing and treating asparagus stem blight.

[0048] Example 2

[0049] The same as in Example 1, except that the mass ratio of the active ingredient 1, the active ingredient 2, the active ingredient 3 and the adjuvant is 1:5:4:1.

[0050] Example 3

[0051] The same as in Example 1, except that the mass ratio of the active ingredient 1, the active ingredient 2, the active ingredient 3 and the adjuvant is 1:4:6:1.

[0052] Example 4

[0053] The same as in Example 1, except that the mass ratio of the active ingredient 1, the active ingredient 2, the active ingredient 3 and the adjuvant is 1:4:7:1.

[0054] Comparative Example 1

[0055] The same as in Example 1, except that the active ingredient 1 is inactivated fermentation broth of Microdochium aestivum and inactivated fermentation broth of Bacillus velezensis, and the mass ratio of the two is 1:1.

[0056] Comparative Example 2

[0057] The same as in Example 1, except that the active ingredient 1 is inactivated fermentation broth of Microdochium aestivum and inactivated fermentation broth of Bacillus amyloliquefaciens, and the mass ratio of the two is 1:1.

[0058] Comparative Example 3

[0059] The same as in Example 1, except that the mass ratio of the active ingredient 1, the active ingredient 2, the active ingredient 3 and the adjuvant is 2:3:5:1.

[0060] Comparative Example 4

[0061] The same as in Example 1, except that the mass ratio of the active ingredient 1, the active ingredient 2, the active ingredient 3 and the adjuvant is 1:1:1:1.

[0062] Comparative Example 5

[0063] The same as in Example 1, except that the active ingredient 2 is omitted.

[0064] Comparative Example 6

[0065] The same as in Example 1, except that the active ingredient 1 is omitted.

[0066] Comparative Example 7

[0067] The same as in Example 1, except that the active ingredient 3 is omitted.

[0068] Comparative Example 8

[0069] The same as in Example 1, except that the active ingredient 2 and the active ingredient 3 are omitted.

[0070] Comparative Example 9

[0071] The same as Example 1, except that active ingredient 1 and active ingredient 3 are omitted.

[0072] Comparative Example 10

[0073] The same as Example 1, except that active ingredient 1 and active ingredient 2 are omitted.

[0074] Experimental Example

[0075] In order to verify the efficacy of the suspending formulations prepared in Examples 1-4 and Comparative Examples 1-10 in preventing and treating asparagus stem blight, a prevention and treatment test of asparagus stem blight was carried out. Asparagus plantations planted in 2022 and with initial onset of stem blight in 2023 were selected, the variety was "Jinggang 701", and equal-area regions were randomly selected, with 50 m 2 , each formulation was repeated 3 times, and a water control group was set up, and the spraying of each region was as follows:

[0076] The suspending formulations prepared in Examples 1-4 and Comparative Examples 1-10 were diluted 500 times and sprayed on the surface of asparagus plants; the dosage of the diluted suspension was 30 L / acre, and spraying was carried out every 15 days, a total of 3 times, and the disease prevention effect before and 45 days after spraying was recorded, 50 plants were randomly selected in each test area, the number of plants at each level was recorded, and the disease index and control effect were calculated, and the disease index and control effect calculation formula is as follows:

[0077] Disease index = ∑(number of plants at each level x corresponding level value) / (total number of plants surveyed x 4) x 100; according to "DB13T1342-2010 Asparagus Stem Blight Grading Standard" for asparagus grading;

[0078] Control effect = (disease index of control area - disease index of treatment area) / disease index of control area x 100%.

[0079] The number of plants at each level of different treatments is shown in Table 1, and the disease index and control effect are shown in Table 2.

[0080] Table 1 Number of plants at each level of different treatments (plants)

[0081]

[0082] Table 2 Disease index and control effect statistics

[0083]

[0084] It can be known from Table 1 and Table 2 that the control effect of the suspension pesticide provided by the application can reach 94.95%-98.99% compared with the control, which shows that the suspension pesticide provided by the application can effectively control asparagus stem blight. Meanwhile, the strains used in the application are screened, and it is found that when micro-white Streptomyces and Bacillus pumilus are combined, the control effect can be improved; the proportion of the effective components is screened, and it is found that after changing the amount of each effective component, the efficacy of the three effective components will be reduced, and the effect will be significantly reduced. Meanwhile, in order to prove the synergistic effect among the three, the comparative examples (Comparative Example 6-Comparative Example 10) in which one component or two components are omitted are set, and the disease index and the control effect are investigated, although the control effect is also increased compared with the control group, but it is still far lower than the control effect of Example 1-Example 4.

[0085] As can be known from the above, the suspension pesticide provided by the application can effectively control asparagus stem blight, and the control effect can reach 94.95%-98.99%. The microorganism, chemical medicine and mixed extract of Myristica and Acanthopanax senticosus are combined for the first time in the application, and it is found that the three have the best effect under a specific proportion, and the synergistic effect among the three is proved by the examples. Therefore, the application provides a new control method, which can reduce the amount of chemical agents and reduce environmental pollution.

[0086] The above-described examples only describe the preferred modes of the application, and do not limit the scope of the application, and various modifications and improvements of the technical solutions of the application made by those skilled in the art without departing from the design spirit of the application shall fall within the protection scope determined by the claims of the application.

Claims

1. A suspension agent for controlling asparagus stem blight, characterized in that, The effective component 1, the effective component 2, the effective component 3 and the auxiliary material are mixed to obtain the suspension pesticide. The effective component 1 is inactivated fermentation liquor of Microtetraena pervelata and inactivated fermentation liquor of Bacillus pumilus; the effective component 2 is mancozeb; the effective component 3 is mixed extraction of Myristica and Acanthopanax senticosus; the auxiliary material is humectant, dispersant, tackifier, anti-freezing agent and organic solvent; The mass ratio of the effective component 1, the effective component 2, the effective component 3 and the auxiliary material is 1:(3-5):(4-7):1; The mass ratio of the inactivated fermentation liquor of Microtetraena pervelata and the inactivated fermentation liquor of Bacillus pumilus in the effective component 1 is 1:1; The mass ratio of the humectant, the dispersant, the tackifier, the anti-freezing agent and the organic solvent is 3:2:1:1:2; The preparation method of the mixed extraction of Myristica and Acanthopanax senticosus is as follows: Myristica and Acanthopanax senticosus are mixed according to a mass ratio of 1:1, and then ethanol extraction is performed to obtain the mixed extraction of Myristica and Acanthopanax senticosus.

2. The suspension pharmaceutical formulation of claim 1, wherein, The humectant is alkylphenol polyoxyethylene ether; the dispersant is sodium tripolyphosphate; the tackifier is polypropylene glycol; the anti-freezing agent is ethylene glycol; and the organic solvent is dipropylene glycol methyl ether.

3. The method of producing a suspension pharmaceutical preparation according to claim 1 or 2, characterized by, The method comprises the following steps: The effective component 1, the effective component 2, the effective component 3 and the auxiliary material are mixed to obtain the suspension pesticide.

4. The suspension pesticide of claim 1 or 2 is used for preventing and treating stem blight of asparagus.

5. A method of controlling stem blight of asparagus, characterized by, The method comprises the following steps: The suspension pesticide of claim 1 or 2 is diluted and then sprayed on the surface of asparagus plants.

6. The method of claim 5, wherein, The dilution multiple is 500.

7. The method of claim 5, wherein, The use amount of the obtained suspension diluent after dilution is 30 L / acre.

Images