Device and method for detecting antibacterial property of antibacterial coating

Abstract

The invention relates to the technical field of coating detection, in particular to an antibacterial property detection device and method for an antibacterial coating, and the device comprises a main body which comprises a cavity with an opening at one end, and the cavity is provided with a bottom surface for placing a to-be-detected sample contained in the cavity from the opening; at least part of the spraying assembly is arranged in the cavity, and the spraying direction of the spraying assembly faces the bottom face; the ultraviolet lamp is arranged in the cavity, and the light direction of the ultraviolet lamp faces the bottom surface; and the detection assembly is at least partially arranged in the cavity. According to the technical scheme provided by the invention, a to-be-detected sample is placed on the bottom surface, a culture solution of fluorescent protein escherichia coli is sprayed, the to-be-detected sample is used as a matrix to culture escherichia coli, fluorescent protein is excited by an ultraviolet lamp, and the antibacterial property of the antibacterial coating is quantitatively detected according to the fluorescence intensity detected by the detection assembly.

Description

Technical Field

[0001] The present invention relates to the technical field of coating detection, and particularly to an antibacterial property detection device and a detection method for antibacterial coatings. Background Art

[0002] Coatings refer to a class of liquid or solid materials that can form a thin film on the surface of an object to provide protection, decoration, or other special functions under certain conditions. Since most early coatings were mainly made from vegetable oils, they were also called paints. With the rapid development of social economy, people have further requirements for their living and residential environments and no longer satisfy with the single decorative function of coatings. They hope that coatings have various new functions, especially expect coatings to have functions such as mildew and antibacterial resistance.

[0003] Currently, in the existing technology, most enterprises are unable to conduct antibacterial property tests by themselves. For the developed products, they need to rely on professional testing institutions for testing. The testing costs are relatively high and the time is relatively long, resulting in an extended product R & D time and increased R & D costs. Summary of the Invention

[0004] The purpose of the present invention is to provide an antibacterial property detection device and a detection method for antibacterial coatings, aiming to solve the problems that the antibacterial property test costs of existing antibacterial coatings are relatively high and the time is relatively long.

[0005] To achieve the above purpose, the present invention provides an antibacterial property detection device for antibacterial coatings, including:

[0006] A main body, including a cavity with an open end, and the cavity has a bottom surface for placing a sample to be tested placed into the cavity from the opening;

[0007] A spraying assembly, at least partially disposed in the cavity, and the spraying direction of the spraying assembly faces the bottom surface for spraying a culture solution of fluorescent protein Escherichia coli onto the bottom surface;

[0008] An ultraviolet lamp, disposed in the cavity, and the light direction of the ultraviolet lamp faces the bottom surface; and,

[0009] A detection assembly, at least partially disposed in the cavity, for detecting the light intensity in the cavity.

[0010] Optionally, the antibacterial property detection device for antibacterial coatings further includes a controller, and the controller can control the spraying time of the spraying assembly, the wavelength of the ultraviolet lamp, and the irradiation time of the ultraviolet lamp.

[0011] Optionally, the wavelength range of the ultraviolet lamp is 200 - 500 nm.

[0012] Optionally, the detection assembly includes:

[0013] A light-sensitive module, disposed in the cavity, for converting an optical signal into an electrical signal;

[0014] An adjustment module, connected to the light-sensitive module, for adjusting the light flux entering the light-sensitive module; and,

[0015] A calculation module, electrically connected to the light-sensitive module, for calculating the light intensity according to the electrical signal.

[0016] Optionally, the detection component includes an image sensor, and the image sensor is disposed in the cavity.

[0017] Optionally, the spraying component includes:

[0018] A nozzle, disposed in the cavity, and the spraying direction of the nozzle faces the bottom surface; and,

[0019] A tank body, communicating with the nozzle.

[0020] The present invention further provides a method for detecting the antibacterial property of an antibacterial coating, using the antibacterial property detection device of the antibacterial coating as described above, including the following steps:

[0021] S10. Place the cured plate coated with the coating on the bottom surface of the cavity, wherein the side coated with the coating faces away from the bottom surface;

[0022] S20. Turn on the ultraviolet lamp, adjust the wavelength range of the ultraviolet lamp to 200 - 300 nm, and irradiate the coating of the plate for 30 - 60 min;

[0023] S30. Spray the culture solution of Escherichia coli with fluorescent protein onto the coating of the plate through the spraying component;

[0024] S40. Place the antibacterial property detection device in an environment of 30 - 40 °C and culture for 4 - 24 h;

[0025] S50. Turn on the ultraviolet lamp, and irradiate the coating of the plate for 3 - 5 min to excite the fluorescent protein to emit fluorescence;

[0026] S60. Detect the fluorescence intensity on the plate, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity.

[0027] Optionally, in step S30, the Escherichia coli with fluorescent protein includes Escherichia coli with green fluorescent protein; and / or,

[0028] The concentration of the culture solution of Escherichia coli with fluorescent protein is 10 6 ~10 8 / mL.

[0029] Optionally, step S60 includes:

[0030] S601. After the ultraviolet lamp is turned off, turn on the detection component to convert the optical signal in the cavity into an electrical signal;

[0031] S602. Calculate the fluorescence intensity in the cavity based on the electrical signal;

[0032] S603. Evaluate the antibacterial effect of the antibacterial coating based on the fluorescence intensity.

[0033] Optionally, in step S601, the detection component is turned on for 10 - 30 s.

[0034] In the technical solution provided by the present invention, the sample to be tested is placed on the bottom surface, and the culture solution of fluorescent protein Escherichia coli is sprayed. Using the sample to be tested as a matrix to culture Escherichia coli, and exciting the fluorescent protein with an ultraviolet lamp. According to the fluorescence intensity measured by the detection component, the antibacterial performance of the antibacterial coating is quantitatively detected. Description of the Drawings

[0035] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on the structures shown in these drawings.

[0036] Figure 1 Schematic diagram of an antibacterial property detection device for an antibacterial coating provided by the present invention;

[0037] Figure 2 Fluorescent physical map of the experimental group and the control group in Embodiment 6 of the present invention.

[0038] Explanation of the reference numerals in the drawings

[0039] Label Name Label Name 100 Antibacterial property detection device for antibacterial coating 2 Nozzle 1 Main body 3 Ultraviolet lamp 11 Opening 4 Cover

[0040] The realization, functional features, and advantages of the object of the present invention will be further described in conjunction with the embodiments with reference to the drawings. Detailed Embodiments

[0041] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention.

[0042] It should be noted that for those not specified with specific conditions in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For reagents or instruments without indicating the manufacturer, they are all conventional products that can be obtained through commercial purchase. In addition, the meaning of "and / or" appearing throughout the text includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, or solution B, or the solution where both A and B are satisfied simultaneously. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0043] In the current prior art, most enterprises are unable to conduct antibacterial performance tests by themselves. For the developed products, they need to rely on professional testing institutions for testing. The testing costs are relatively high and the time is relatively long, resulting in an extended product R & D time and increased R & D costs.

[0044] In view of this, please refer to Figure 1 , the present invention provides an antibacterial property detection device 100 for antibacterial coatings, comprising: a main body 1 including a cavity with an opening 11 at one end, the cavity having a bottom surface for placing a sample to be tested placed into the cavity from the opening 11; a spraying assembly, at least partially disposed in the cavity, the spraying direction of the spraying assembly facing the bottom surface for spraying a culture solution of Escherichia coli with fluorescent protein onto the bottom surface; an ultraviolet lamp 3 disposed in the cavity, the light direction of the ultraviolet lamp 3 facing the bottom surface; and a detection assembly, at least partially disposed in the cavity for detecting the light intensity in the cavity.

[0045] It should be noted that the spraying assembly includes at least one spraying outlet, and multiple spraying outlets can be provided; the detection assembly can use a light intensity sensor as the detection component or a camera as the detection component, as long as it can detect the fluorescence emitted by the fluorescent protein; the fluorescent protein of Escherichia coli with fluorescent protein can be green fluorescent protein, or red fluorescent protein, or yellow fluorescent protein or cyan fluorescent protein, as long as it can emit fluorescence after absorbing light of a specific wavelength. Specifically, in some embodiments of the present invention, green fluorescent protein is used, which can emit green fluorescence of 509 nm under ultraviolet light of 395 nm.

[0046] In the technical solution provided by the present invention, the sample to be tested is placed on the bottom surface, and the culture solution of fluorescent protein Escherichia coli is sprayed. The Escherichia coli is cultured with the sample to be tested as the matrix, and the fluorescent protein is excited by the ultraviolet lamp 3. According to the fluorescence intensity measured by the detection component, the antibacterial performance of the antibacterial coating is quantitatively detected. The greater the measured light intensity, the more Escherichia coli is cultured, and the lower the antibacterial performance of the antibacterial coating. On the contrary, the smaller the measured light intensity, the higher the antibacterial performance of the antibacterial coating, thereby realizing the quantitative detection of the antibacterial performance of the antibacterial coating.

[0047] In addition, the antibacterial property detection device 100 of the antibacterial coating further includes a cover body 4, which is arranged on the main body 1 and can cover the opening 11. With such a setting, the cover body 4 can cover the opening 11 so that ambient light does not enter the cavity from the opening 11, reducing the influence of ambient light on the detection result.

[0048] Furthermore, the antibacterial property detection device 100 of the antibacterial coating further includes a controller, which can control the spraying time of the spraying component, the wavelength of the ultraviolet lamp 3, and the irradiation time of the ultraviolet lamp 3.

[0049] With such a setting, the controller can adjust the spraying time according to the size of the bottom surface and the number of spraying outlets in the spraying component, reducing the error caused by manual addition of the culture solution and avoiding the reduction of detection accuracy; it can also adjust the wavelength and irradiation time of the ultraviolet lamp 3 before spraying to improve the sterilization effect, and adjust the wavelength and irradiation time of the ultraviolet lamp 3 after culturing for a period of time to excite the fluorescent protein to emit fluorescence, so that the antibacterial property detection device can automatically complete the antibacterial detection, reducing manual operation and improving the operation convenience.

[0050] Furthermore, the wavelength range of the ultraviolet lamp 3 is 200-500 nm. By setting the wavelength range of the ultraviolet lamp 3 at 200-300 nm, the sterilization effect of the ultraviolet lamp 3 can be improved. Specifically, in some embodiments of the present invention, the wavelength of the ultraviolet lamp 3 during sterilization is set at 250-260 nm; by setting the wavelength range of the ultraviolet lamp 3 at 300-500 nm, the fluorescent protein can be excited to emit fluorescence. Specifically, in some embodiments of the present invention, the wavelength of the ultraviolet lamp 3 during excitation is set at 390-400 nm.

[0051] Furthermore, the detection component includes: a photosensitive module, which is arranged in the cavity and is used to convert the optical signal into an electrical signal; an adjustment module, which is connected to the photosensitive module and is used to adjust the light flux entering the photosensitive module; and a calculation module, which is electrically connected to the photosensitive module and is used to calculate the light intensity according to the electrical signal.

[0052] It should be noted that the photosensitive module can be a single photoelectric sensor or an image sensor formed by multiple photoelectric sensors. When the photosensitive module is set as an image sensor, the antibacterial performance of the coating can be evaluated according to the fluorescence protein image on the sample to be measured; the adjustment module can be a filter or a lens. The filter can absorb light of some wavelengths and only allow light of a specific wavelength to pass through. The lens can adjust the focal length or aperture. One of the filter and the lens can be selected, or both can be set at the same time. When both are set at the same time, the focal length and aperture can be adjusted and the light intensity within a specific wavelength range can be detected only, reducing the physical error caused by inconsistent parameters when detecting different samples. At the same time, detecting the light intensity within a specific wavelength range can avoid the detection error caused by stray light, thus avoiding the reduction of detection accuracy.

[0053] By using the photosensitive module and the calculation module in cooperation, the fluorescence emitted by the fluorescent protein can be converted into an electrical signal, and the light intensity of the fluorescence can be calculated. The greater the light intensity, the more Escherichia coli are cultured, and the lower the antibacterial performance of the antibacterial coating; by using the photosensitive module and the adjustment module in cooperation, the focal length can be adjusted according to the parameters of the sample to be measured so that the focal length is equal to the distance between the photosensitive module and the surface of the sample to be measured, reducing the detection error caused by different thicknesses of the sample to be measured and improving the detection accuracy. Also, a suitable filter can be selected according to the wavelength of the fluorescence excited by the fluorescent protein to reduce the detection error caused by ambient light and further improve the detection accuracy.

[0054] Furthermore, the detection component includes an image sensor, and the image sensor is arranged in the cavity. With such a setting, the optical image on the photosensitive surface of the image sensor is converted into an electrical signal that is in a corresponding proportional relationship with the optical image. By analyzing the electrical signal, the signal intensity of the optical image can be obtained, and the distribution of fluorescent protein Escherichia coli on the surface of the coating can be obtained, facilitating the analysis of the change of the antibacterial performance intensity of the coating at different positions on the substrate.

[0055] Furthermore, the spraying component includes: a nozzle 2 arranged in the cavity, and the spraying direction of the nozzle 2 faces the bottom surface; and a tank body communicating with the nozzle 2. It can be understood that multiple nozzles 2 can be set to increase the spraying area, speed up the spraying speed, and reduce the detection error caused by large time differences in spraying different samples and inconsistent culture durations. With such a setting, the culture solution of fluorescent protein Escherichia coli can be added to the tank body and then sprayed onto the bottom surface through the nozzle 2, thereby reducing manual operation and improving the convenience of operation.

[0056] The present invention also provides a method for detecting the antibacterial property of an antibacterial coating, which uses the antibacterial property detection device of the antibacterial coating as described above, and includes the following steps: S10. Place the cured plate coated with the coating on the bottom surface of the cavity, wherein the side coated with the coating faces away from the bottom surface; S20. Turn on the ultraviolet lamp, adjust the wavelength range of the ultraviolet lamp to 200-300 nm, and irradiate the bottom surface for 30-60 min; S30. Spray the culture solution of Escherichia coli with fluorescent protein onto the coating of the plate through the spraying assembly; S40. Place the antibacterial property detection device in an incubator at 30-40 °C for 4-24 h; S50. Turn on the ultraviolet lamp and irradiate the coating of the plate for 3-5 min to excite the fluorescent protein to emit fluorescence; S60. Detect the fluorescence intensity on the coating of the plate and the surface of the bottom surface, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity.

[0057] It should be noted that in step S20, the irradiation duration of the ultraviolet lamp can be 30 min, 40 min, 50 min or 60 min; in step S40, the culture temperature can be 30 °C, 35 °C, 37 °C or 40 °C, and the culture time can be 4 h, 6 h, 12 h or 24 h; in step S50, the wavelength range of the ultraviolet lamp covers the fluorescence wavelength excited by the Escherichia coli with fluorescent protein, and the wavelength of the ultraviolet lamp can be 400 nm, 420 nm, 440 nm, 460 nm, 480 nm or 500 nm. Specifically, in some embodiments of the present invention, green fluorescent protein labeling is used, and fluorescence can be excited under ultraviolet light of 395 nm. In step S50, the wavelength range of the ultraviolet lamp is set to 390-400 nm.

[0058] By first sterilizing with an ultraviolet lamp, culturing Escherichia coli with fluorescent protein, and then exciting the fluorescent protein to emit fluorescence, the contact between the human body and the sample to be tested or the bacterial culture solution during the detection process is reduced, the error caused by manual operation is reduced, the detection accuracy is improved, and the operation convenience is improved at the same time; by adjusting the wavelength range of the ultraviolet lamp to 200-300 nm and irradiating the coating of the plate for 30-60 min in step S20, the sterilization effect of the ultraviolet lamp can be improved, and the effect of complete sterilization can be achieved after 30-60 min, avoiding the influence of miscellaneous bacteria in the device on the detection result and the reduction of detection accuracy.

[0059] Further, in step S30, the Escherichia coli with fluorescent protein includes Escherichia coli with green fluorescent protein; and / or, the concentration of the culture solution of Escherichia coli with fluorescent protein is 10 6 ~10 8 / mL. By using Escherichia coli with green fluorescent protein, green fluorescence can be excited under ultraviolet light of 390-400 nm; by using a concentration of 10 6 ~10 8The Escherichia coli culture solution of fluorescent protein at [X] / mL can cultivate a large number of Escherichia coli in a short time, so that the coating with strong antibacterial property can significantly inhibit the increase in the number of Escherichia coli, while the coating with weak antibacterial property has poor inhibitory effect, thereby improving the discrimination of different samples to be detected.

[0060] Further, step S60 includes: S601. After the ultraviolet lamp is turned off, turn on the detection component to convert the optical signal in the cavity into an electrical signal; S602. Calculate the fluorescence intensity in the cavity according to the electrical signal; S603. Evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity. By converting the fluorescence emitted by the fluorescent protein into an electrical signal and calculating the light intensity of the fluorescence, the greater the measured light intensity, the more Escherichia coli are cultivated, and the lower the antibacterial performance of the antibacterial coating. On the contrary, the smaller the measured light intensity, the higher the antibacterial performance of the antibacterial coating, thus realizing the quantitative detection of the antibacterial performance of the antibacterial coating.

[0061] Further, in step S601, the opening duration of the detection component is 10 - 30 s. With such a setting, the fluorescence duration excited by the Escherichia coli of fluorescent protein can be completely detected, and the antibacterial effect can be evaluated from the perspective of the fluorescence duration, improving the accuracy and comprehensiveness of the antibacterial performance detection.

[0062] The technical solution of the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0063] Example 1

[0064] Example 1 provides a method for detecting the antibacterial property of an antibacterial coating, including the following steps:

[0065] Use the inorganic antibacterial decorative coating of Guangzhou Boyingte New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it, control the thickness of the cured coating layer to be 0.1 mm as the experimental group, place the cured stainless steel plate on the bottom surface of the antibacterial property detection device, and close the lid;

[0066] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water, heat it to 100 °C and cool to form a culture medium. Inoculate the Escherichia coli strain with green fluorescent protein on the culture medium and cultivate it at 37 °C for 18 h. Then, add the cultivated Escherichia coli strain to the dilution solution (containing 0.5% beef extract, 1% agar, 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL and mix evenly, and add it to the tank;

[0067] Note: There are some placeholders like "[X]" and " 6 ", " 8 " in the original text which seem to be incomplete or have some specific context not fully provided. The translation is done as accurately as possible based on the available text.Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm and irradiate the coating on the stainless steel plate. After 40 min, turn off the ultraviolet lamp;

[0068] Open the nozzle and spray the culture solution of green fluorescent protein Escherichia coli in the tank onto the coating on the stainless steel plate for 20 s;

[0069] Place the antibacterial property detection device in an incubator at 37 °C for 6 h;

[0070] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm and irradiate the coating on the stainless steel plate for 3 min;

[0071] Turn off the ultraviolet lamp, detect the fluorescence intensity on the surface of the stainless steel plate through a photoelectric sensor, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0072] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0073] Example 2

[0074] Example 2 provides a method for detecting the antibacterial property of an antibacterial coating, including the following steps:

[0075] Use the inorganic antibacterial decorative coating of Guangzhou Boyingte New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it. Control the thickness of the cured coating layer to be 0.02 mm as the experimental group. Place the cured stainless steel plate on the bottom surface of the antibacterial property detection device and close the lid;

[0076] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water, heat it to 100 °C and cool it to form a culture medium. Inoculate the green fluorescent protein Escherichia coli strain onto the culture medium and incubate it at 37 °C for 18 h. Then, add the cultured Escherichia coli strain to the diluent (containing 0.5% beef extract, 1% agar, 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL and mix evenly, then add it to the tank;

[0077] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm and irradiate the coating on the stainless steel plate. After 40 min, turn off the ultraviolet lamp;

[0078] Open the nozzle and spray the culture solution of green fluorescent protein Escherichia coli in the tank onto the coating on the stainless steel plate for 20 s;

[0079] Place the antibacterial property detection device in an incubator at 37 °C for 6 h;

[0080] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm and irradiate the coating on the stainless steel plate for 3 min;

[0081] Turn off the ultraviolet lamp, detect the fluorescence intensity on the surface of the stainless steel plate through a photoelectric sensor, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0082] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0083] Example 3

[0084] Example 3 provides a method for detecting the antibacterial property of an antibacterial coating, including the following steps:

[0085] Use the inorganic antibacterial decorative coating of Guangzhou Boyingte New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it. Control the thickness of the cured coating layer to be 0.1 mm as the experimental group. Place the cured stainless steel plate on the bottom surface of the antibacterial property detection device, and close the lid;

[0086] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water, heat it to 100 °C and cool it to form a culture medium. Inoculate the Escherichia coli strain with green fluorescent protein on the culture medium and culture it at 37 °C for 18 h. Then, add the cultured Escherichia coli strain to the diluent (containing 0.5% beef extract, 1% agar, 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL and mix evenly, then add it to the tank;

[0087] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm and irradiate the coating on the stainless steel plate for 40 min, then turn off the ultraviolet lamp;

[0088] Open the nozzle and spray the culture solution of Escherichia coli with green fluorescent protein in the tank onto the coating on the stainless steel plate for 20 s;

[0089] Place the antibacterial property detection device in an incubator at 37 °C for 4 h;

[0090] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm and irradiate the coating on the stainless steel plate for 3 min;

[0091] Turn off the ultraviolet lamp, detect the fluorescence intensity on the surface of the stainless steel plate through a photoelectric sensor, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0092] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0093] Example 4

[0094] Example 4 provides a method for detecting the antibacterial property of an antibacterial coating, comprising the following steps:

[0095] Use the inorganic antibacterial decorative coating of Guangzhou Boyint New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it. Control the thickness of the cured coating layer to be 0.1 mm as the experimental group. Place the cured stainless steel plate on the bottom surface of the antibacterial property detection device, and close the lid;

[0096] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water, heat it to 100 °C and cool it to form a culture medium. Inoculate the Escherichia coli strain with green fluorescent protein onto the culture medium and culture it at 37 °C for 18 h. Then, add the cultured Escherichia coli strain to the dilution solution (containing 0.5% beef extract, 1% agar, 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL and mix evenly, then add it to the tank;

[0097] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm and irradiate the coating on the stainless steel plate for 40 min, then turn off the ultraviolet lamp;

[0098] Open the nozzle and spray the culture solution of Escherichia coli with green fluorescent protein in the tank onto the coating on the stainless steel plate for 20 s;

[0099] Place the antibacterial property detection device in an incubator at 37 °C for 24 h;

[0100] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm and irradiate the coating on the stainless steel plate for 3 min;

[0101] Turn off the ultraviolet lamp, detect the fluorescence intensity on the surface of the stainless steel plate through a photoelectric sensor, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0102] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0103] Example 5

[0104] Example 5 provides a method for detecting the antibacterial property of an antibacterial coating, comprising the following steps:

[0105] Use the inorganic antibacterial decorative coating of Guangzhou Boyint New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it. Control the thickness of the cured coating layer to be 0.1 mm as the experimental group. Place the cured stainless steel plate on the bottom surface of the antibacterial property detection device, and close the lid;

[0106] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water. Heat it to 100 °C and then cool it to form a culture medium. After inoculating the Escherichia coli strain with green fluorescent protein onto the culture medium and culturing it at 37 °C for 18 h, add the cultured Escherichia coli strain to a diluent (containing 0.5% beef extract, 1% agar, and 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL, then mix evenly and add it to the said tank;

[0107] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm and irradiate the coating on the stainless steel plate for 40 min, then turn off the ultraviolet lamp;

[0108] Open the nozzle and spray the culture solution of Escherichia coli with green fluorescent protein in the said tank onto the coating on the stainless steel plate for 20 s;

[0109] Place the antibacterial property detection device at 37 °C and culture it for 6 h;

[0110] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm and irradiate the coating on the stainless steel plate for 5 min;

[0111] Turn off the ultraviolet lamp, detect the fluorescence intensity on the surface of the stainless steel plate through a photoelectric sensor, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0112] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0113] Example 6

[0114] Example 6 provides a method for detecting the antibacterial property of an antibacterial coating, including the following steps:

[0115] Use the inorganic antibacterial decorative coating of Guangzhou Boyinte New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it. Control the thickness of the cured coating layer to be 0.1 mm as the experimental group. Place the cured stainless steel plate on the bottom surface of the antibacterial property detection device and close the lid;

[0116] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water. Heat it to 100 °C and then cool it to form a culture medium. After inoculating the Escherichia coli strain with green fluorescent protein onto the culture medium and culturing it at 37 °C for 18 h, add the cultured Escherichia coli strain to a diluent (containing 0.5% beef extract, 1% agar, and 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL, then mix evenly and add it to the said tank;

[0117] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm and irradiate the coating on the stainless steel plate. After 60 minutes, turn off the ultraviolet lamp;

[0118] Open the nozzle and spray the culture solution of Escherichia coli with green fluorescent protein in the tank onto the coating on the stainless steel plate for 20 s;

[0119] Place the antibacterial property detection device in an incubator at 37 °C for 6 h;

[0120] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm and irradiate the coating on the stainless steel plate for 3 min;

[0121] Turn off the ultraviolet lamp, take pictures of the coating surface with a camera, detect the fluorescence intensity on the surface of the stainless steel plate, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0122] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0123] Comparative Example 1

[0124] Comparative Example 1 provides a method for detecting the antibacterial property of an antibacterial coating, including the following steps:

[0125] Use the inorganic antibacterial decorative coating of Guangzhou Boyingte New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it. Control the thickness of the cured coating layer to be 0.1 mm as the experimental group. Place the cured stainless steel plate on the bottom surface of the antibacterial property detection device and close the lid;

[0126] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water, heat it to 100 °C and cool to form a culture medium. Inoculate the Escherichia coli strain with green fluorescent protein into the culture medium and incubate it at 37 °C for 18 h. Then, add the cultured Escherichia coli strain to a diluent (containing 0.5% beef extract, 1% agar, 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL and mix evenly, then add it to the tank;

[0127] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm and irradiate the coating on the stainless steel plate for 20 minutes, then turn off the ultraviolet lamp;

[0128] Open the nozzle and spray the culture solution of Escherichia coli with green fluorescent protein in the tank onto the coating on the stainless steel plate for 20 s;

[0129] Place the antibacterial property detection device in an incubator at 37 °C for 6 h;

[0130] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm, and irradiate the coating on the stainless steel plate for 3 minutes;

[0131] Turn off the ultraviolet lamp, detect the fluorescence intensity on the surface of the stainless steel plate through a photoelectric sensor, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0132] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0133] Comparative Example 2

[0134] Comparative Example 2 provides a method for detecting the antibacterial property of an antibacterial coating, including the following steps:

[0135] Use the inorganic antibacterial decorative coating of Guangzhou Boyingte New Materials Co., Ltd., evenly coat it on a 304 stainless steel plate of 100*100*0.2 mm and cure it. Control the thickness of the cured coating layer to be 0.1 mm as the experimental group. Place the cured stainless steel plate on the bottom surface of the antibacterial property detection device, and close the lid;

[0136] Prepare a solution with beef extract, agar, sodium chloride, peptone, and water, heat it to 100 °C and cool to form a culture medium. Inoculate the Escherichia coli strain with green fluorescent protein on the culture medium and culture it at 37 °C for 18 h. Then, add the cultured Escherichia coli strain to the diluent (containing 0.5% beef extract, 1% agar, 0.85% sodium chloride) and dilute it to 10 6 ~10 8 / mL and mix evenly, then add it to the tank;

[0137] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 254 nm, irradiate the coating on the stainless steel plate for 40 minutes, and then turn off the ultraviolet lamp;

[0138] Open the nozzle and spray the culture solution of Escherichia coli with green fluorescent protein in the tank onto the coating of the stainless steel plate for 20 s;

[0139] Place the antibacterial property detection device in an incubator at 37 °C for 1 h;

[0140] Turn on the ultraviolet lamp, adjust the wavelength of the ultraviolet lamp to 395 nm, irradiate the coating on the stainless steel plate for 3 minutes;

[0141] Turn off the ultraviolet lamp, detect the fluorescence intensity on the surface of the stainless steel plate through a photoelectric sensor, and evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity;

[0142] Take a 304 stainless steel plate of 100*100*0.2 mm as a control and repeat the above steps.

[0143] According to the detection data of Examples 1-5 and Comparative Examples 1-2, record the initial fluorescence intensity and the fluorescence duration within 30 s of the experimental group and the control group in each example or comparative example, and calculate the antibacterial rate. The detection results of Examples 1-5 and Comparative Examples 1-2 are shown in Table 1. Among them, the antibacterial rate = (initial fluorescence intensity of the control group - initial fluorescence intensity of the experimental group) / initial fluorescence intensity of the control group.

[0144] Table 1 Detection Results of Examples 1-5 and Comparative Examples 1-2

[0145]

[0146] It can be seen from Table 1 that the antibacterial rates of Example 1 and Examples 3-6 are all between 66% and 68%, while the antibacterial rate of Example 2 is 53.13%. This is because the thickness of the coating layer in Example 2 is 0.02 mm, while the thicknesses of the coating layers in Example 1 and Examples 3-6 are all 0.1 mm. Therefore, the antibacterial effect of Example 2 is slightly lower than that of Example 1 and Examples 3-6.

[0147] The difference between Comparative Example 1 and the example is only that in the sterilization stage of Comparative Example 1, the ultraviolet lamp irradiation duration is 20 min, while the ultraviolet lamp irradiation duration of Example 1 is 40 min. The difference in the fluorescence duration of the experimental group and the control group in Comparative Example 1 is not significant, and the antibacterial rate of Comparative Example 1 is 19.55%, which is significantly lower than the antibacterial rate of Example 1, 67.97%. This shows that the short ultraviolet irradiation time fails to completely sterilize, resulting in a reduction in the accuracy of quantitatively detecting the antibacterial performance.

[0148] The difference between Comparative Example 2 and the example is only that the culture duration of Comparative Example 2 is 1 hour, while the culture duration of Example 1 is 6 hours. The antibacterial rate in Comparative Example 2 is 9.22%, which has a significant difference from the detection result of Example 1. This shows that the difference in the number of Escherichia coli cultures is not significant due to the too short culture time, and an accurate antibacterial effect cannot be obtained.

[0149] According to the physical pictures of the experimental group and the control group taken by the camera in Example 6 as Figure 2 shown, it Figure 2 is not difficult to see that the fluorescence intensity of the control group is significantly higher than that of the experimental group, indicating that the antibacterial performance of the experimental group is stronger than that of the control group; in addition, the fluorescence intensity in the central area of the experimental group is slightly lower than that in the edge area, indicating that in the experimental group, the antibacterial performance of the antibacterial coating in the central area of the stainless steel plate is stronger than that in the edge area.

[0150] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. All equivalent structural transformations made under the inventive concept of the present invention by using the content of the specification and drawings of the present invention, or directly / indirectly applied to other related technical fields are included in the patent protection scope of the present invention.

Claims

1. An antibacterial property detection device for an antibacterial coating, characterized in that: include: The main body comprises a cavity with an opening at one end, wherein the cavity has a bottom surface for placing a sample to be tested placed in the cavity through the opening; A spray component is at least partially disposed in the cavity, the spray component sprays toward the bottom surface, and is used to spray the culture solution of fluorescent protein Escherichia coli onto the bottom surface; An ultraviolet lamp is disposed in the cavity, and the light direction of the ultraviolet lamp is toward the bottom surface; and a detection component is at least partially disposed in the cavity and is used to detect the light intensity in the cavity.

2. The antibacterial property detection device of the antibacterial coating according to claim 1, characterized in that: The antibacterial property detection device of the antibacterial coating also includes a controller, which can control the spraying time of the spraying component, the wavelength of the ultraviolet lamp and the irradiation time of the ultraviolet lamp.

3. The antibacterial property detection device of the antibacterial coating according to claim 1, characterized in that: The wavelength range of the ultraviolet lamp is 200-500nm.

4. The antibacterial property detection device of the antibacterial coating according to claim 1, characterized in that: The detection component comprises: A photosensitive module, disposed in the cavity, for converting optical signals into electrical signals; an adjusting module, connected to the photosensitive module, and used for adjusting the light flux entering the photosensitive module; and The calculation module is electrically connected to the photosensitive module and is used to calculate the light intensity according to the electrical signal.

5. The antibacterial property detection device of the antibacterial coating according to claim 1, characterized in that: The detection component includes an image sensor, and the image sensor is arranged in the cavity.

6. The antibacterial property detection device of the antibacterial coating according to claim 1, characterized in that: The spray assembly comprises: a nozzle, disposed in the cavity, the spraying direction of the nozzle being toward the bottom surface; and, The tank body is connected to the nozzle.

7. A method for detecting the antibacterial property of an antibacterial coating, using the antibacterial property detection device of an antibacterial coating as claimed in any one of claims 1 to 6, characterized in that: The following steps are involved: S10, placing the cured plate coated with the paint on the bottom surface of the cavity, wherein the side coated with the paint faces away from the bottom surface; S20, turning on the ultraviolet lamp, adjusting the wavelength of the ultraviolet lamp to 200-300 nm, and irradiating the coating of the plate for 30-60 minutes; S30, spraying the culture solution of fluorescent protein Escherichia coli onto the coating of the plate through the spray component; S40, culturing the antibacterial detection device at 30-40° C. for 2-4 hours; S50, turning on the ultraviolet lamp to irradiate the coating of the plate for 3 to 5 minutes to excite the fluorescent protein to emit fluorescence; S60, detecting the fluorescence intensity on the surface of the plate, and evaluating the antibacterial effect of the antibacterial coating according to the fluorescence intensity.

8. The method for detecting the antibacterial property of the antibacterial coating according to claim 6, wherein: In step S30, the fluorescent protein Escherichia coli includes green fluorescent protein Escherichia coli; and / or, The concentration of fluorescent protein Escherichia coli culture solution is 10 6 ~10 8 / mL.

9. The method for detecting the antibacterial property of the antibacterial coating according to claim 6, wherein: Step S60 includes: S601, after the ultraviolet lamp is turned off, the detection component is turned on to convert the optical signal in the cavity into an electrical signal; S602, calculating the fluorescence intensity in the cavity according to the electrical signal; S603. Evaluate the antibacterial effect of the antibacterial coating according to the fluorescence intensity.

10. The method for detecting the antibacterial property of the antibacterial coating according to claim 9, characterized in that: In step S601, the detection component is turned on for 10 to 30 seconds.

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