An automated inoculation system for liquid edible fungi spawn production

Abstract

This invention provides an automated inoculation system for the production of liquid spawn for edible fungi. The system includes a liquid spawn production module, an inoculation module, and a control system. The liquid spawn production module encompasses a culture medium preparation unit, a sterilization unit, an inoculation unit, and a cultivation unit, automating the entire process from culture medium preparation to liquid spawn cultivation. It provides precise control at each stage, reducing the risk of contamination and ensuring stable spawn quality. The inoculation module consists of an inoculation robot, inoculation needles, and a culture container conveyor line. The inoculation robot precisely controls the inoculation needles to achieve uniform inoculation and improve inoculation efficiency. The control system controls the operation of each module based on data collected by sensors. Furthermore, the system has remote monitoring and operation capabilities. This invention automates the production and inoculation of liquid spawn for edible fungi, improving production efficiency and product quality, and offers significant economic benefits and promising applications.

Description

Technical Field

[0001] This invention relates to the field of edible fungi production technology, and more specifically, to an automated inoculation system for producing liquid edible fungi spawn. Background Technology

[0002] Edible fungi, as a type of agricultural product with high economic and nutritional value, have received widespread attention and popularity globally. In recent years, with the continuous growth of market demand, the edible fungi industry has developed rapidly. Liquid spawn technology, as a key technology in edible fungi cultivation, has gradually become a research hotspot and development direction in the field of edible fungi production due to its advantages of rapid germination, rapid growth, and uniform mycelial age. However, there are still many problems to be solved in the production and inoculation process of liquid spawn for edible fungi, which seriously restricts the further promotion and application of this technology.

[0003] Low automation is a significant problem in the production of liquid microbial cultures. Traditional methods rely heavily on manual weighing and mixing of culture media, which is not only labor-intensive and inefficient but also makes it difficult to ensure consistency and accuracy of composition across batches, thus affecting the growth and quality of the liquid cultures. During sterilization, the control of key parameters such as temperature, pressure, and time is often imprecise, leading to incomplete or over-sterilization. Incomplete sterilization results in contamination by other microorganisms, while over-sterilization can destroy nutrients in the culture medium and reduce the viability of the microorganisms. Inoculation is typically performed in open or semi-open environments, and manual inoculation inevitably introduces external microorganisms, increasing the risk of contamination. Furthermore, the lack of effective automated equipment makes it impossible to guarantee the efficiency and accuracy of the inoculation process.

[0004] During the cultivation stage, existing cultivation equipment has limited capabilities in monitoring and regulating environmental parameters. Environmental factors such as temperature, humidity, and oxygen content are crucial for the growth of liquid microbial cultures, but traditional cultivation equipment often cannot control these parameters in real time and with precision, leading to an unstable growth environment that affects the growth rate and quality of the cultures. Furthermore, manual operation makes it difficult to achieve continuous and detailed monitoring of the cultivation process, hindering the timely detection and handling of abnormalities, further reducing the success rate of liquid microbial culture production.

[0005] In the inoculation process, existing inoculation methods suffer from uneven inoculation amounts. During manual inoculation, due to differences in the skill level and experience of operators, it is difficult to ensure a consistent inoculation amount at each inoculation point. This leads to inconsistent growth rates and significant yield variations in the edible fungi, affecting the quality and economic benefits of the final product. Furthermore, manual inoculation is slow and cannot meet the needs of large-scale production, thus limiting the large-scale development of the edible fungi industry.

[0006] Existing liquid spawn production and inoculation technologies for edible fungi suffer from numerous shortcomings, including low automation, high risk of contamination by other microorganisms, uneven inoculation rates, and low production efficiency. There is an urgent need to develop a more advanced, efficient, and stable automated production and inoculation system to promote the sustainable development of the edible fungi industry. Therefore, we propose an automated production and inoculation system for liquid spawn of edible fungi. Summary of the Invention

[0007] The purpose of this invention is to address the problems raised in the existing background technology. To achieve the above-mentioned objective, this invention provides the following technical solution: an automated production and inoculation system for liquid edible fungi spawn, comprising a liquid spawn production module, an inoculation module, and a control system; the liquid spawn production module includes a culture medium preparation unit, a sterilization unit, an inoculation unit, and a cultivation unit; the culture medium preparation unit is used to prepare the culture medium, the sterilization unit is used to sterilize the culture medium, the inoculation unit is used to inoculate the mother culture into the sterilized culture medium, and the cultivation unit is used to cultivate the liquid spawn;

[0008] The inoculation module includes an inoculation robot, an inoculation needle, and a culture container conveyor line. The inoculation robot is used to control the inoculation needle to inoculate the cultured liquid culture into the culture container, and the culture container conveyor line is used to transport the culture container to be inoculated. The control system is used to control the operation of the liquid culture production module and the inoculation module.

[0009] As a preferred technical solution of the present invention, the culture medium preparation unit includes a raw material storage tank, a metering device, a mixing tank, and a conveying pipeline. The raw material storage tank is used to store culture medium raw materials, the metering device is used to accurately measure the amount of each raw material, the mixing tank is used to mix the raw materials to form a culture medium, and the conveying pipeline is used to convey the mixed culture medium to the sterilization unit.

[0010] As a preferred technical solution of the present invention, the measuring device is a high-precision electronic scale with a precision of milligrams.

[0011] As a preferred technical solution of the present invention, the sterilization unit adopts a high temperature and high pressure sterilization device, which is equipped with multiple safety protection devices such as over-temperature protection and over-pressure protection.

[0012] As a preferred technical solution of the present invention, the inoculation unit includes a strain storage tank, an inoculation gun, and a sterile inoculation chamber. The strain storage tank is used to store the mother culture, and the inoculation gun inoculates the mother culture into the sterilized culture medium in the sterile inoculation chamber.

[0013] As a preferred technical solution of the present invention, the culture unit includes multiple culture tanks, each of which is equipped with a temperature sensor, a humidity sensor, an oxygen sensor and a stirring device. The control system adjusts the temperature, humidity and oxygen content in the culture tank according to the data fed back by the sensors. The stirring device is used to fully mix the culture medium and the inoculum.

[0014] As a preferred technical solution of the present invention, the inoculation robot is a six-axis industrial robot that controls the movement trajectory and inoculation depth of the inoculation needle.

[0015] As a preferred technical solution of the present invention, the control system includes a central processing unit, sensors, and actuators. The sensors are distributed in key parts of the liquid culture production module and the inoculation module, and are used to collect environmental parameters and equipment operating status information. The central processing unit issues control commands based on the collected information, and the actuators control the operation of each unit and device according to the control commands.

[0016] As a preferred technical solution of the present invention, the control system also has remote monitoring and operation functions, allowing operators to remotely monitor the system's operating status and make operational adjustments via the Internet.

[0017] As a preferred embodiment of the present invention, the inoculation needle is connected to the culture tank of the culture unit through an infusion tube, and the cultured liquid bacteria are transported to the inoculation needle.

[0018] Compared with existing technologies, the beneficial effects of this invention are as follows: The system of this invention automates the entire process of liquid culture production and inoculation. Starting with culture medium preparation, precise metering devices and automated mixing processes avoid the tediousness and errors of manual operation, enabling rapid and stable completion of culture medium preparation. Sterilization, inoculation, and cultivation are also automated, significantly shortening the production cycle. In the inoculation module, the inoculation robot continuously and efficiently completes the inoculation task, greatly improving speed compared to manual inoculation and significantly enhancing overall production efficiency.

[0019] In terms of quality stability, the system demonstrates significant advantages. The precise preparation of the culture medium ensures a consistent growth environment for the liquid culture, laying the foundation for cultivating high-quality strains. Multiple safety protections and precise parameter control in the sterilization unit effectively kill unwanted microorganisms and spores, reducing the risk of contamination. The inoculation process takes place in a sterile inoculation chamber, minimizing interference from external microorganisms. The culture unit monitors and adjusts environmental parameters in real time using sensors, providing stable and suitable conditions for strain growth, ensuring consistent quality, uniform bacterial age, and strong growth vigor of the liquid culture.

[0020] In terms of inoculation precision, the inoculation robot controls the movement trajectory and inoculation depth of the inoculation needle, ensuring a uniform inoculation amount in each culture container. This results in uniform development of the edible fungi during subsequent growth, reducing growth differences and improving the overall quality and yield of the product.

[0021] Furthermore, the remote monitoring and operation functions of the control system allow operators to monitor the system's operating status anytime, anywhere, promptly identify and resolve problems, and facilitate system maintenance and management. Moreover, the automated operation of the entire system reduces manual labor intensity and labor costs, resulting in significant economic benefits and promising market application prospects. Attached Figure Description

[0022] Figure 1 The system logic block diagram provided for this invention;

[0023] Figure 2 The system module unit block diagram provided by the present invention;

[0024] Figure 3 The system composition block diagram provided by the present invention;

[0025] Figure 4 A block diagram illustrating the characteristics of system units provided by this invention;

[0026] Figure 5 The system functional block diagram provided by the present invention. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0028] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely illustrates some embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the embodiments of the present invention can be combined with each other. It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0029] Example 1: An automated inoculation system for producing liquid edible fungi spawn, comprising a liquid spawn production module, an inoculation module, and a control system; the liquid spawn production module includes a culture medium preparation unit, a sterilization unit, an inoculation unit, and a cultivation unit; the culture medium preparation unit is used to prepare the culture medium, the sterilization unit is used to sterilize the culture medium, the inoculation unit is used to inoculate the mother culture into the sterilized culture medium, and the cultivation unit is used to cultivate the liquid spawn;

[0030] The inoculation module includes an inoculation robot, an inoculation needle, and a culture container conveyor line. The inoculation robot is used to control the inoculation needle to inoculate the cultured liquid culture into the culture container, and the culture container conveyor line is used to transport the culture containers to be inoculated. The control system is used to control the operation of the liquid culture production module and the inoculation module.

[0031] The culture medium preparation unit includes a raw material storage tank, a metering device, a mixing tank, and a conveying pipeline. The raw material storage tank is used to store the culture medium raw materials, the metering device is used to accurately measure the amount of each raw material, the mixing tank is used to mix the raw materials to form the culture medium, and the conveying pipeline is used to transport the mixed culture medium to the sterilization unit.

[0032] The measuring device is a high-precision electronic scale with an accuracy of milligrams.

[0033] The sterilization unit uses high-temperature and high-pressure sterilization equipment, which is equipped with multiple safety protection devices, including over-temperature protection and over-pressure protection.

[0034] The inoculation unit includes a culture storage tank, an inoculation gun, and a sterile inoculation chamber. The culture storage tank is used to store the mother culture, and the inoculation gun is used in the sterile inoculation chamber to inoculate the mother culture into the sterilized culture medium.

[0035] The cultivation unit includes multiple cultivation tanks, each equipped with a temperature sensor, a humidity sensor, an oxygen sensor, and a stirring device. The control system adjusts the temperature, humidity, and oxygen content inside the cultivation tank based on the data fed back from the sensors, and the stirring device is used to ensure that the culture medium and the inoculum are thoroughly mixed.

[0036] The inoculation robot is a six-axis industrial robot that controls the movement trajectory and inoculation depth of the inoculation needle.

[0037] The control system includes a central processing unit, sensors, and actuators. The sensors are distributed in key parts of the liquid culture production module and the inoculation module to collect environmental parameters and equipment operating status information. The central processing unit issues control commands based on the collected information, and the actuators control the operation of each unit and device according to the control commands.

[0038] The control system also has remote monitoring and operation functions, allowing operators to remotely monitor the system's operating status and make operational adjustments via the Internet.

[0039] The inoculation needle is connected to the culture tank of the culture unit through an infusion tube, which delivers the cultured liquid bacteria to the inoculation needle.

[0040] Python code for an automated inoculation system for liquid edible fungi spawn production:

[0041] import time

[0042] Liquid microbial culture production module

[0043] class LiquidStrainProduction:

[0044] def__init__(self):

[0045] self.culture_medium_prepared=False

[0046]

[0047] print("Please complete the sterilization process first.")

[0048] def culture_strain(self):

[0049] if self.inoculated:

[0050] print("Culturing liquid bacteria")

[0051] time.sleep(5) simulates the incubation time.

[0052] self.cultured = True

[0053] print("Liquid culture complete.")

[0054] else:

[0055] print("Please complete the vaccination process first.")

[0056] Inoculation module

[0057] class InoculationModule:

[0058] def__init__(self):

[0059] self.ready = False

[0060] def prepare_for_inoculation(self):

[0061] print("The vaccination module is preparing")

[0062] time.sleep(2) simulates preparation time

[0063] self.ready = True

[0064] print("The vaccination module is ready.")

[0065] def perform_inoculation(self,liquid_strain):

[0066] if self.ready and liquid_strain.cultured:

[0067] print("Initiating vaccination procedure")

[0068] time.sleep(3) simulates vaccination time

[0069] print("Vaccination operation completed.")

[0070]

[0071] Code explanation:

[0072] The LiquidStrainProduction class simulates the production of liquid microbial cultures, comprising four steps: culture medium preparation, sterilization, inoculation, and cultivation. Each step has a corresponding method, and flags are used to ensure that the operations are performed in sequence.

[0073] The `InoculationModule` class simulates inoculation and contains two methods: `Prepare` and `Execute`. Inoculation can only be performed when preparation is complete and the liquid culture is finished.

[0074] The ControlSystem class is a simulation control system responsible for coordinating the operation of the liquid culture production module and the inoculation module.

[0075] Main program: Creates a control system instance, sequentially calls the methods of the production process and the inoculation process, and completes the entire automated production inoculation process.

[0076] Example 2: An automated inoculation system for liquid edible fungi spawn production, comprising a liquid spawn production module, an inoculation module, and a control system. The liquid spawn production module includes a culture medium preparation unit, a sterilization unit, an inoculation unit, and a cultivation unit. The culture medium preparation unit includes a raw material storage tank, a metering device, a mixing tank, and a conveying pipeline. The raw material storage tank stores various culture medium raw materials, and the metering device measures the amount of each raw material, conveying it to the mixing tank for thorough mixing to form a homogeneous culture medium. The conveying pipeline transports the mixed culture medium to the sterilization unit. The sterilization unit employs high-temperature, high-pressure sterilization equipment, such as a high-pressure steam sterilizer. This equipment can thoroughly sterilize the culture medium, killing any contaminating microorganisms and spores. The temperature, pressure, and time parameters during the sterilization process are precisely controlled by the control system. The inoculation unit includes a spawn storage tank, an inoculation gun, and a sterile inoculation chamber. The spawn storage tank stores high-quality mother culture, and the inoculation gun inoculates the mother culture into the sterilized culture medium in the sterile inoculation chamber. The inoculation process is carried out in a sterile environment, effectively avoiding contamination by other microorganisms. Culture unit: It is equipped with multiple culture tanks, each containing a temperature sensor, humidity sensor, oxygen sensor, and stirring device.

[0077] The control system adjusts the temperature, humidity, and oxygen content environmental parameters inside the incubator in real time based on data feedback from sensors, providing suitable conditions for the growth of liquid microorganisms. The stirring device ensures thorough mixing of the culture medium and microorganisms, promoting their growth and reproduction. The control system includes a central processing unit (CPU), sensors, and actuators. Sensors are distributed at key locations in the liquid microorganism production and inoculation modules, collecting real-time environmental parameters such as temperature, pressure, humidity, and oxygen content, as well as equipment operating status information, and transmitting this information to the CPU. The CPU analyzes and processes the received information, issuing control commands according to a preset program. These commands then control the operation of each unit and device via actuators, achieving automated operation of the entire system. The metering device in the culture medium preparation unit uses a high-precision electronic scale with an accuracy of milligrams, ensuring accurate proportions of the culture medium raw materials.

[0078] The high-temperature and high-pressure sterilization equipment in the sterilization unit is equipped with multiple safety protection devices, such as over-temperature protection and over-pressure protection, to ensure the safety and reliability of the sterilization process.

[0079] The inoculation robot is a six-axis industrial robot with high flexibility and precision, capable of adapting to the inoculation needs of culture containers of different sizes and shapes.

[0080] The control system is also equipped with remote monitoring and operation functions, allowing operators to monitor the system's operating status anytime, anywhere via the Internet, and to perform remote operation and adjustments.

[0081] An automated production and inoculation system for liquid edible fungi spawn, including the operation process and functions of each module.

[0082] 1. Liquid culture production module

[0083] 1.1 Culture medium preparation unit

[0084] Raw material storage tank: for storing glucose, peptone, and agar culture medium raw materials.

[0085] Measuring device: A high-precision electronic scale (accurate to the milligram level) is used to automatically weigh raw materials according to the formula.

[0086] Mixing tank: Add the weighed raw materials to water and stir evenly to form a culture medium.

[0087] Delivery pipeline: The mixed culture medium is delivered to the sterilization unit through the pipeline.

[0088] 1.2 Sterilization Unit

[0089] The culture medium was sterilized using a high-temperature, high-pressure sterilization device (121℃, 0.1MPa).

[0090] The equipment is equipped with over-temperature protection (alarm and stop heating if the temperature exceeds 125℃) and over-pressure protection (pressure relief if the pressure exceeds 0.12MPa) to ensure safety.

[0091] 1.3 Inoculation Unit

[0092] Microbial culture storage tank: Used to store mother cultures that have passed inspection.

[0093] Sterile inoculation room: Provides a sterile environment to prevent contamination.

[0094] Inoculation gun: The mother culture is inoculated into the sterilized culture medium, and the operation is completed automatically by the control system.

[0095] 1.4 Culture Unit

[0096] It contains multiple stainless steel culture tanks, each containing:

[0097] Temperature sensor: monitors and reports temperature data.

[0098] Humidity sensor: monitors and reports humidity data.

[0099] Oxygen sensor: monitors and reports oxygen content.

[0100] Stirring device: to ensure that the culture medium and the bacterial strain are thoroughly mixed.

[0101] The control system adjusts the environmental parameters inside the incubator (such as temperature 28℃±1℃, humidity 90%±5%, and sufficient oxygen content) based on sensor data to ensure the normal growth of the liquid bacteria.

[0102] 2. Inoculation Module

[0103] 2.1 Inoculation Robot

[0104] A six-axis industrial robot is used to precisely control the movement trajectory and inoculation depth of the inoculation needle.

[0105] The inoculation needle is connected to the culture tank of the culture unit through an infusion tube to extract the cultured liquid bacteria.

[0106] 2.2 Culture Container Conveyor Line

[0107] Transport the culture containers (such as glass bottles or plastic bags) to the inoculation station.

[0108] The containers are identified by barcode scanning to ensure accurate dosage and injection location.

[0109] 2.3 Vaccination process

[0110] The inoculation robot controls the inoculation needle to insert into the culture container and inject a quantitative amount of liquid bacteria.

[0111] After injection, the conveyor line removes the inoculated container, ready for the next process.

[0112] 3. Control System

[0113] 3.1 Central Processing Unit

[0114] The core control unit receives sensor data and issues control commands.

[0115] Achieve fully automated control of the liquid culture production module and the inoculation module.

[0116] 3.2 Sensors

[0117] Distributed in key locations, they collect environmental parameters (such as temperature, humidity, and oxygen) and equipment operating status information in real time.

[0118] Data is transmitted to the central processing unit via wireless or wired means.

[0119] 3.3 Actuator

[0120] The central processing unit controls the operation of each unit device according to the instructions issued by the central processing unit (such as starting the stirring device and moving the conveyor line).

[0121] 3.4 Remote Monitoring and Operation

[0122] Operators can access the control system via the Internet to check the system's operating status.

[0123] It supports remote adjustment of parameters (such as temperature, humidity, and inoculation volume) to improve management efficiency.

[0124] 4. Workflow Example

[0125] Step 1: Culture medium preparation

[0126] The raw material storage tank is automatically fed, and the metering device weighs the raw materials.

[0127] The mixing tank is stirred to form a culture medium, which is then delivered to the sterilization unit through a pipeline.

[0128] Step 2: Sterilization

[0129] High-temperature and high-pressure sterilization equipment sterilizes the culture medium, which is then cooled for later use.

[0130] Step 3: Inoculate the mother animal

[0131] In a sterile inoculation room, the inoculation gun is used to inoculate the mother culture into the sterilized culture medium.

[0132] Step 4: Liquid culture

[0133] The environment inside the culture tank is adjusted to a suitable level (temperature 28℃, humidity 90%, sufficient oxygen), and the stirring device is continuously operated.

[0134] Cultivate the liquid culture until it matures.

[0135] Step 5: Inoculate into the culture container

[0136] The inoculation robot controls the inoculation needle to draw out the cultured liquid bacteria and inject it into the culture container to be inoculated.

[0137] The culture container conveyor line automatically completes the conveying and positioning.

[0138] Step 6: Remote Monitoring

[0139] Operators can check the system's operating status via computer or mobile phone and make remote adjustments when necessary.

[0140] High degree of automation: The entire process from culture medium preparation to inoculation is automated, reducing human intervention.

[0141] Precise control: High-precision electronic scales and six-axis robot equipment ensure operational accuracy.

[0142] High safety: The sterilization unit has multiple layers of protection, and the sterile inoculation room prevents contamination.

[0143] Remote management: Supports remote monitoring and operation via the Internet, improving management efficiency.

[0144] The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described in the present invention. Although the present invention has been described in detail with reference to the above embodiments, the present invention is not limited to the specific embodiments described above. Therefore, any modifications or substitutions to the present invention, and all technical solutions and improvements that do not depart from the spirit and scope of the invention, are covered within the scope of the claims of the present invention.

Claims

1. An automated inoculation system for producing liquid edible fungi spawn, characterized in that, The system includes a liquid culture production module, an inoculation module, and a control system. The liquid culture production module includes a culture medium preparation unit, a sterilization unit, an inoculation unit, and a culture unit. The culture medium preparation unit is used to prepare the culture medium, the sterilization unit is used to sterilize the culture medium, the inoculation unit is used to inoculate the mother culture into the sterilized culture medium, and the culture unit is used to culture the liquid culture. The inoculation module includes an inoculation robot, an inoculation needle, and a culture container conveyor line. The inoculation robot is used to control the inoculation needle to inoculate the cultured liquid culture into the culture container, and the culture container conveyor line is used to transport the culture container to be inoculated. The control system is used to control the operation of the liquid culture production module and the inoculation module. The culture medium preparation unit includes a raw material storage tank, a metering device, a mixing tank, and a conveying pipeline. The raw material storage tank is used to store culture medium raw materials, the metering device is used to accurately measure the amount of each raw material, the mixing tank is used to mix the raw materials to form the culture medium, and the conveying pipeline is used to convey the mixed culture medium to the sterilization unit. The measuring device is a high-precision electronic scale with an accuracy of milligrams. The sterilization unit uses a high-temperature and high-pressure sterilization device, which is equipped with multiple safety protection devices including over-temperature protection and over-pressure protection. The inoculation unit includes a culture storage tank, an inoculation gun, and a sterile inoculation chamber. The culture storage tank is used to store the mother culture, and the inoculation gun inoculates the mother culture into the sterilized culture medium in the sterile inoculation chamber. The culture unit includes multiple culture tanks, each equipped with a temperature sensor, a humidity sensor, an oxygen sensor, and a stirring device. The control system adjusts the temperature, humidity, and oxygen content inside the culture tank based on the data fed back by the sensors. The stirring device is used to ensure that the culture medium and the inoculum are fully mixed.

2. The automated inoculation system for liquid edible fungi spawn production according to claim 1, characterized in that, The inoculation robot is a six-axis industrial robot that controls the movement trajectory and inoculation depth of the inoculation needle.

3. The automated inoculation system for liquid edible fungi spawn production according to claim 1, characterized in that, The control system includes a central processing unit, sensors, and actuators. The sensors are distributed in key parts of the liquid culture production module and the inoculation module to collect environmental parameters and equipment operating status information. The central processing unit issues control commands based on the collected information, and the actuators control the operation of each unit and device according to the control commands.

4. The automated inoculation system for liquid edible fungi spawn production according to claim 3, characterized in that, The control system also has remote monitoring and operation functions, allowing operators to remotely monitor the system's operating status and make operational adjustments via the Internet.

5. The automated inoculation system for liquid edible fungi spawn production according to claim 1, characterized in that, The inoculation needle is connected to the culture tank of the culture unit through an infusion tube, which delivers the cultured liquid bacteria to the inoculation needle.

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