Povidone-iodine solution adopting lipidosome wrapping technology and preparation method of povidone-iodine solution

Abstract

A povidone-iodine solution adopting a liposome wrapping technology belongs to the technical field of medicine preparation, and comprises povidone-iodine, a humectant, a buffer agent, a stabilizer, a thickener, a liposome raw material, a surfactant and pure water. The preparation method of the povidone-iodine solution by adopting the liposome wrapping technology comprises two processes of liposome raw material preparation and povidone-iodine solution preparation, and the liposome raw material preparation and povidone-iodine solution preparation respectively comprise five processes. Under the combined action of related raw materials and a preparation process, when an obtained finished product is applied to disinfection operation of dairy cow breasts and the like, waste caused by iodine sublimation volatilization and oxidation is reduced, instantaneous sterilization and long-acting antibacterial effects are guaranteed, pollution of external environment to the breasts can be isolated, breast skin can be nourished for a long time, and the breast health care effect is improved. The skin cannot be stimulated, and the subsequent breast cleaning process is facilitated, so that a favorable technical support is provided for the health of dairy cow breasts and the like. In conclusion, the method has a good application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid medicines, in particular to a povidone iodine solution using liposome encapsulation technology and a preparation method thereof. Background Art

[0002] Povidone iodine is a complex of polyvinylpyrrolidone and iodine, and is a non-ionic iodophor with active iodine as the main bactericidal component. It has a strong killing effect on bacteria, viruses, fungi, molds, and spores. Currently, it is the most widely used and safest antibacterial disinfectant, with characteristics such as high efficiency, broad spectrum, small irritation, no toxic side effects, no residue, and no drug resistance. It is a veterinary medicine widely used in ranch breeding.

[0003] With the improvement of ranch management level and considering from the perspective of food safety, in recent years in the aquaculture field, there has been a trend towards using low-concentration iodine medicated bath solutions. The test results from multiple parties also show that an effective iodine concentration of about 700 ppm is sufficient to kill common mastitis pathogens such as cows within 30 seconds; however, products with low iodine content have problems such as unstable iodine components and easy sublimation and oxidation. Especially for low-iodine products added with emollient components, the thermal stability of their effective iodine content is even worse. After being applied to the medicated bath of cow teats, etc., it cannot effectively solve the skin health problems of cow teats, etc. Summary of the Invention

[0004] In order to overcome the drawbacks described in the background art due to the limitations of the composition and preparation process of the existing povidone iodine bath solution applied to the disinfection of cow udders, etc., the present invention provides a povidone iodine solution using liposome encapsulation technology and a preparation method thereof, which, under the combined action of relevant raw materials and preparation processes, when the obtained finished product is applied to the disinfection operation of cow udders, etc., reduces the waste caused by iodine sublimation, volatilization, and oxidation, ensures instantaneous bactericidal and long-term antibacterial effects, can isolate the pollution of the external environment to the udder, nourish the udder skin persistently, will not cause irritation to the skin, and is conducive to the subsequent cleaning process of the udder, thereby providing a favorable technical support for the health of cow udders, etc.

[0005] The technical solution adopted by the present invention to solve its technical problems is: A povidone iodine solution using liposome encapsulation technology, comprising povidone iodine, a humectant, a buffer, a stabilizer, a thickener, liposome raw materials, a surfactant, and pure water; the amount of povidone iodine is 2%-2.5%, the amount of the humectant is 5%-15%, the amount of the buffer is 1%-3%, the amount of the stabilizer is 0.5%-1%, the amount of the thickener is 0.2%-0.6%, the amount of the liposome raw materials is 2%-5%, the amount of the surfactant is 2%-5%, and the amount of pure water is 11%-32%; the humectant is at least one of diglycerol, glycerol, butanediol, sorbitol, betaine, PCA-NA; the buffer is at least one of sodium citrate, sodium lactate, sodium phosphate, sodium ascorbate; the stabilizer is at least one of potassium iodide, potassium chloride, sodium chloride; the thickener is at least one of cellulose, scleroglucan, polyethylene glycol; the surfactant is one of cocoamidopropyl betaine, dodecyldimethylamine oxide, fatty alcohol polyoxyethylene ether; the liposome raw materials are composed of lecithin, phytosterol, and an emulsifier; the surfactant is one of Tween 80, Span 60, poloxamer.

[0006] Furthermore, the povidone iodine is of pharmaceutical grade, the humectant, buffer, thickener, and liposome raw materials are of food grade, and the stabilizer and surfactant are of AR reagent grade.

[0007] Furthermore, the lecithin is any one of soy lecithin, hydrogenated soy lecithin, egg yolk lecithin, preferably egg yolk lecithin.

[0008] Furthermore, the phytosterol is any one of β-sitosterol, stigmasterol, ergosterol.

[0009] A preparation method of povidone iodine solution using liposome encapsulation technology, including two processes: liposome raw material preparation and povidone iodine solution preparation; the liposome raw material preparation includes the following steps, S1: In a high-pressure homogenization reactor, add pure water, diglycerol, glycerol, lecithin, phytosterol, and Tween raw materials respectively, and then heat; S2: Start the stirrer equipped with the high-pressure homogenization reactor, and the stirrer stirs and mixes various raw materials in the reactor; S3: Start the high-pressure homogenization mechanism equipped with the high-pressure homogenization reactor, and various raw materials pass through the narrow gaps of the dispersion unit of the high-pressure homogenization mechanism under pressure. The pressure inside various raw materials drops to form a supersonic flow rate, and the particles inside various raw materials collide, cavitate, and leak, forming a complete homogeneous state; S4: The external circulation pump of the high-pressure homogenization reactor pumps the raw materials in the reactor out from the lower end and inputs them from the upper end of the reactor. The above process is repeated multiple times to avoid unevenness of various raw materials up and down in the reactor and improve the mixing effect of various raw materials; S5: After various homogenizations and stirrings are completed, cool to obtain liposome raw materials, and discharge and inspect for later use; the povidone iodine solution preparation includes the following steps, A1: Add pure water, humectant, buffer, and stabilizer to the reactor, start the stirrer of the reactor, and the stirrer stirs various raw materials until they are completely dissolved; A2: Add thickener to the reactor and continue to stir to ensure that the thickener is completely swollen, and then cool to room temperature; A3: Add povidone iodine to the reactor and continue to stir until povidone iodine is completely dispersed; A4: Add liposome raw materials and surfactant to the reactor, and the stirring shaft of the stirrer continues to stir until the raw materials are evenly dispersed to obtain a povidone iodine solution using liposome encapsulation technology; A5: Release the povidone iodine solution obtained in step A4 from the reactor, discharge and inspect, and fill.

[0010] Further, in step S1, the heating temperature is 50 - 80 °C; in step S2, the rotational speed of the stirring shaft of the stirrer is 10 - 20 rpm.

[0011] Further, in step A1, the rotational speed of the stirring shaft of the stirrer is 10 - 20 rpm.

[0012] Further, in step A2, the rotational speed of the stirring shaft of the stirrer is 10 - 30 rpm, heat to 75 - 85 °C during stirring, and maintain for 25 - 35 min.

[0013] Further, in step A3, the stirring time is 8 - 12 min.

[0014] Further, in step A4, the rotational speed of the stirring shaft of the stirrer is 20 - 30 rpm, and the stirring time is 8 - 12 min.

[0015] The beneficial effects of the present invention compared with the prior art are as follows: (1) Since liposomes are used to encapsulate povidone iodine, iodine can be effectively stabilized, reducing the waste caused by the sublimation, volatilization and oxidation of iodine, optimizing the use efficiency of iodine, and comparable to the bactericidal effect of high-concentration iodine; (2) The free iodine content in the liposome-encapsulated povidone iodine is the same as that in the high-content povidone iodine solution (reaching dozens of ppm), ensuring the instantaneous bactericidal effect during application; (3) The iodine encapsulated in the liposome will aggregate and fuse as the zeta potential of the external environment of the liposome decreases, thereby releasing active iodine to supplement the insufficient active iodine in the solution, making the povidone iodine solution have a long-term antibacterial effect; (4) During the aggregation and fusion of the liposomes, the skin care and nourishing components therein are released, which synergize with the bionic skin film components in the povidone iodine solution to gradually form a breathable and water-locking protective film on the surface of the cow's teat skin, etc., isolating the pollution of the external environment, and the skin care components in the povidone iodine solution and the liposomes can nourish the skin persistently: (5) The liposome raw materials themselves also originate from natural products, with high safety and good compatibility with the cow's skin, etc., and will not cause irritation to the skin: (6) During the drying process, the povidone iodine solution can form a breathable and skin-friendly film outside the cow's udder, has good water solubility, and is easily removed during the next water cleaning process of the cow's udder, achieving a high cleaning effect. The present invention can provide favorable technical support for the health of the cow's udder, etc. In summary, the present invention has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a flowchart of a preparation method of a povidone iodine solution using a liposome encapsulation technique. DETAILED DESCRIPTION OF THE INVENTION

[0017] A povidone iodine solution using liposome encapsulation technology, comprising povidone iodine, a humectant, a buffer, a stabilizer, a thickener, liposome raw materials, a surfactant, and pure water; the amount of povidone iodine is 2% - 2.5%, the amount of the humectant is 5% - 15%, the amount of the buffer is 1% - 3%, the amount of the stabilizer is 0.5% - 1%, the amount of the thickener is 0.2% - 0.6%, the amount of the liposome raw materials is 2% - 5%, the amount of the surfactant is 2% - 5%, and the amount of pure water is 11% - 32%; the humectant is at least one of diglycerol, glycerol, butanediol, sorbitol, betaine, PCA-NA; the buffer is at least one of sodium citrate, sodium lactate, sodium phosphate, sodium ascorbate; the stabilizer is at least one of potassium iodide, potassium chloride, sodium chloride; the thickener is at least one of cellulose, scleroglucan, polyethylene glycol; the surfactant is one of cocoamidopropyl betaine, dodecyldimethylamine oxide, fatty alcohol polyoxyethylene ether; the liposome raw materials are composed of lecithin, phytosterol, and an emulsifier; the surfactant is one of Tween 80, Span 60, poloxamer.

[0018] The povidone iodine is of pharmaceutical grade, the humectant, buffer, thickener, liposome raw materials, etc. are of food grade, and the stabilizer and surfactant are of AR reagent grade. The lecithin is any one of soybean lecithin, hydrogenated soybean lecithin, egg yolk lecithin, preferably egg yolk lecithin. The phytosterol is any one of β-sitosterol, stigmasterol, ergosterol.

[0019] Figure 1As shown in the figure, a preparation method of povidone iodine solution using liposome encapsulation technology includes two processes: liposome raw material preparation and povidone iodine solution preparation. The liposome raw material preparation includes the following steps: (1): In a 50L high-pressure homogenization reactor, add the aqueous phase moisturizing components composed of various raw materials (where pure water is 20kg, diglycerol is 9kg, and glycerol is 6kg) and the oil phase phospholipid components composed of various raw materials (where lecithin is 6kg, phytosterol is 6kg, and Tween 80 is 3kg), and heat to 65°C; (2): Start the stirrer equipped with the high-pressure homogenization reactor. The stirrer stirs and mixes the aqueous phase moisturizing components and the oil phase phospholipid components composed of various raw materials in the reactor. The rotational speed of the stirrer shaft is 10-20 rpm; (3): Start the stirrer equipped with the high-pressure homogenization reactor. The stirrer stirs and mixes various raw materials in the reactor. Under pressure, various raw materials pass through the narrow gaps of the dispersion unit of the high-pressure homogenization mechanism, and the pressure inside various raw materials drops to form a supersonic flow rate. The particles inside various raw materials collide, cavitate, and leak, forming a complete homogeneous state; (4): The external circulation pump of the high-pressure homogenization reactor pumps the raw materials in the reactor out from the lower end and inputs them from the upper end of the reactor. The above process is repeated multiple times (the time is 30 minutes) to avoid unevenness of various raw materials up and down in the reactor and improve the mixing effect of various raw materials; (5): After various homogenization stirrings are completed, cool (cool to below 30°C) to obtain liposome raw materials, and discharge and inspect them for later use. The povidone iodine solution preparation includes the following steps: (1): Taking a 1000L reactor as an example, according to the formula process, add 812kg of pure water to the reactor, add moisturizing agents (where glycerol is 70kg), buffering agents (sodium lactate is 15kg), and stabilizers (where potassium iodide is 2kg, potassium chloride is 1kg, and sodium chloride is 5kg), start stirring, start the stirrer of the reactor, and the stirrer (the rotational speed of the stirrer shaft is 10-20 rpm) stirs various raw materials until they are completely dissolved; (2): Add the thickening agent (sclerotium gum is 3kg) to the reactor and continue stirring. The rotational speed of the stirrer shaft is 30 rpm. During stirring, heat to 80°C and maintain for 30 minutes to ensure that the thickening agent is completely swollen, and then cool to room temperature; (3): Add povidone iodine (22kg) to the reactor and continue stirring for 10 minutes until the povidone iodine is completely dispersed; (4): Add the liposome raw materials (30kg) and surfactants (where dodecyldimethylamine oxide is 20kg and fatty alcohol polyoxyethylene ether is 20kg) to the reactor. The rotational speed of the stirrer shaft is 30 rpm, and stir for 10 minutes until the raw materials are evenly dispersed to obtain the povidone iodine solution using liposome encapsulation technology; (5): Release the povidone iodine solution obtained in step A4 from the reactor, discharge and inspect it, and then fill it into containers.

[0020] Figure 1As shown, the present invention is mainly used for disinfecting the udders of dairy cows, etc. The prepared povidone iodine solution using liposome encapsulation technology can improve the stability of the povidone iodine solution and optimize the skin care effect. The specific background technical principle is as follows. Liposomes are usually closed spherical structures with a bilayer concentric closed central water cavity formed by self-assembly of lipids. Lipid encapsulation technology is a commonly used drug delivery system. By encapsulating a drug (the povidone iodine solution in the present invention) in the lipid layer, it can increase the stability and biological activity of the encapsulated drug. Liposomes can regulate the release rate and targeting by changing the lipid composition and structure, thereby improving the efficacy of the drug. In application, the intensity of the repulsive electrostatic interaction between naturally charged colloidal particles is generally measured by a Zeta potential meter to evaluate the surface charge of liposomes. If all the particles in the system have a large negative or positive Zeta potential, they will tend to repel each other, so there will be no tendency to aggregate. Generally, it is considered that particles with an absolute Zeta potential greater than 30 mV are stable. The particle size and its distribution of liposomes are directly related to the stability of the preparation (the liquid medicine encapsulated inside), affecting the behavior and disposition of liposomes in the body, and are important parameters for evaluating the stability of liposomes. High-quality liposomes should have a relatively stable particle size and distribution, with a normal distribution of particle size and a narrow distribution range. The Zeta potential is a measure of the strength of the repulsive or attractive interaction between particles in the system. An appropriate zeta potential can reduce the aggregation and fusion of liposomes and has a stabilizing effect between liposomes and drugs. In the breeding industry, the skin health problem of dairy cow udders is ultimately a problem of the skin barrier. The skin barrier generally refers to a brick-wall structure composed of a sebum membrane and the stratum corneum, which is equivalent to being composed of "bricks + mortar". Among them, the bricks are keratinocytes, and the mortar is the intercellular lipid of the stratum corneum cells. Sebum is a lipid present between keratinocytes that has the functions of moisturizing, protecting the skin barrier, and preventing the penetration of harmful substances, that is, the "mortar" in the "brick-wall" structure system of the stratum corneum, and is an important barrier of the skin. The natural moisturizing factors in the sebum membrane can moisturize the skin, prevent the loss of skin moisture, prevent the entry of external harmful substances into the skin, and inhibit the growth of bacteria on the skin surface. The povidone iodine solution prepared by the liposome encapsulation technology based on the bionic skin technology in the present invention has liposomes that simulate the sebum layer of dairy cows in terms of composition and structure, and at the same time supplement the essential lipids of the skin stratum corneum, which can reduce water loss, promote the absorption of active ingredients, effectively lock in skin moisture and nutrients, improve the water content and softness of the stratum corneum, and gradually repair and strengthen the barrier in the long term.

[0021] As shown in Table 1, the povidone iodine solution prepared by the present invention using liposome encapsulation technology was experimentally measured to have a povidone iodine solution concentration of 2%, a pH value of 3.80, and a povidone iodine content of 11.26% of the labeled amount (in the veterinary pharmacopoeia, the available iodine content should be 8.5% - 12.0% of the labeled amount). According to the accelerated test method (Disinfection Technical Specification, 54°C, 14D), the povidone iodine content was measured to be 10.36% of the labeled amount, meeting the requirements of the veterinary pharmacopoeia. Compared with the 2% povidone iodine solution prepared by the traditional method, the thermal stability was significantly improved.

[0022] In the above table, the good stability of the present invention was illustrated. The original content was 11.26%, and after the thermal stability experiment at 54°C for 14 days, it decreased to 10.36%, a decrease of 0.9%. The decrease rate was (11.26 - 10.36) / 11.26 = 7.99%. Generally speaking, the smaller the specification number of the povidone iodine solution, the relatively worse the stability. The traditional povidone iodine solution only requires bactericidal action, has a simple composition, and relatively better stability. Nowadays, general products usually add some skin-moisturizing ingredients, such as glycerin, lanolin, trehalose, etc. However, after adding the skin-moisturizing ingredients, the thermal stability becomes significantly worse.

[0023] The bactericidal and antibacterial experiments were compared between the povidone iodine solution prepared by the present invention using liposome encapsulation technology and the commercially available 7.5% povidone iodine solution. The test results are shown in Table 2 (in Table 2, (Sample No. 001 is 2% povidone iodine solution, and Sample No. 002 is the commercially available 7.5% povidone iodine solution)):

[0024] As can be seen from Table 2, the bactericidal and antibacterial effects of the povidone iodine solution prepared by the present invention using liposome encapsulation technology and the commercially available 7.5% povidone iodine solution on various pathogenic bacteria in the cow udder are basically the same, and can effectively meet the application of bactericidal and antibacterial.

[0025] The long-term antibacterial test experiments were compared between the povidone iodine solution prepared by the present invention using liposome encapsulation technology and the commercially available 7.5% povidone iodine solution. The test results are shown in Table 3:

[0026] As can be seen from Table 3, the 2% povidone iodine solution sample prepared by the present invention using liposome encapsulation technology has a continuous antibacterial effect within 30 minutes, while the commercially available 7.5% sample has an unstable continuous antibacterial effect within 30 minutes; the 2.0% povidone iodine solution sample of the present invention has a continuous antibacterial effect within 60 minutes, while the commercially available 7.5% sample has an unstable continuous antibacterial effect within 60 minutes.

[0027] Figure 1As shown, the finished product obtained by the present invention has the following advantages when applied to disinfection operations such as dairy cow udders. Since liposomes are used to encapsulate povidone iodine, it can effectively stabilize iodine, reduce the waste caused by the sublimation, volatilization and oxidation of iodine, optimize the use efficiency of iodine, and rival the bactericidal effect of high-concentration iodine; the free iodine content in liposome-encapsulated povidone iodine is the same as that in a high-content povidone iodine solution (reaching dozens of ppm), ensuring the instantaneous bactericidal effect during application; the iodine encapsulated in the liposomes will aggregate and fuse as the zeta potential of the external environment of the liposomes decreases, thereby releasing active iodine to supplement the insufficient active iodine in the solution, making the povidone iodine solution have a long-term antibacterial effect; during the aggregation and fusion of the liposomes, the skin-nourishing components therein are released, which synergize with the bionic skin film components in the povidone iodine solution to gradually form a breathable and water-locking protective film on the surface of the dairy cow teat skin, etc., isolating the pollution of the external environment, and the skin-nourishing components in the povidone iodine solution and the liposomes can nourish the skin persistently: the liposome raw materials themselves also originate from natural products, with high safety and good compatibility with dairy cow skin, etc., and will not cause irritation to the skin: during the drying process, the povidone iodine solution can form a breathable and skin-friendly film outside the dairy cow udder, has good water solubility, and is easily removed during the next water cleaning process of the dairy cow udder, achieving a high cleaning effect. The present invention can provide favorable technical support for the health of dairy cow udders, etc.

[0028] The foregoing has shown and described the basic principles, main features and advantages of the present invention. For those skilled in the art, it is obvious that the present invention is limited to the details of the above-described exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, in any regard, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, it is intended to embrace all changes falling within the meaning and scope of the equivalent elements of the claims in the present invention.

[0029] In addition, it should be understood that although this specification is described in accordance with the embodiments, not every embodiment only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in the embodiments can also be appropriately combined to form other embodiments understandable to those skilled in the art.

Claims

1. A povidone-iodine solution using liposome encapsulation technology, characterized in that: The invention comprises povidone iodine, a moisturizing agent, a buffer, a stabilizer, a thickener, a liposome raw material, a surfactant and pure water; the povidone iodine content is 2%-2.5%, the moisturizing agent content is 5%-15%, the buffer content is 1%-3%, the stabilizer content is 0.5%-1%, the thickener content is 0.2%-0.6%, the liposome raw material content is 2%-5%, the surfactant content is 2%-5% and the pure water content is 11%-32%; the moisturizing agent is diglycerol, glycerol, butylene glycol, sorbitol, betaine, PCA- The invention relates to a novel nanostructured carbon foam comprising: a ...

2. The povidone-iodine solution using liposome encapsulation technology according to claim 1, characterized in that: Povidone iodine is of pharmaceutical grade, humectant, buffer, thickener, and liposome raw material are of food grade, and stabilizer and surfactant are of AR reagent grade.

3. The povidone-iodine solution using liposome encapsulation technology according to claim 1, characterized in that: The lecithin may be any one of soybean lecithin, hydrogenated soybean lecithin, and egg yolk lecithin, and egg yolk lecithin is preferred.

4. The povidone-iodine solution using liposome encapsulation technology according to claim 1, characterized in that: The phytosterol is any one of β-sitosterol, stigmasterol and ergosterol.

5. The method for preparing a povidone-iodine solution using liposome encapsulation technology according to any one of claims 1 to 4, characterized in that: The method comprises two processes: preparation of liposome raw materials and preparation of povidone iodine solution. The preparation of liposome raw materials comprises the following steps: S1: adding pure water, diglycerol, glycerol, lecithin, phytosterol and Tween raw materials to a high-pressure homogenization reactor respectively, and then heating; S2: starting a stirrer provided with the high-pressure homogenization reactor, and the stirrer stirs and mixes various raw materials in the reactor; S3: starting a high-pressure homogenization mechanism provided with the high-pressure homogenization reactor, and various raw materials pass through a narrow gap of a dispersion unit of the high-pressure homogenization mechanism under a pressurized state, and the pressure in the various raw materials decreases to form an ultrasonic flow rate, and particles in the various raw materials collide, cavitate and leak to form a completely homogenized state; S4: an external circulation pump of the high-pressure homogenization reactor extracts the raw materials in the reactor from the lower end and inputs them from the upper end of the reactor, and the above process is repeated for multiple times to avoid various raw materials in the reactor. The upper and lower parts are uneven inside and outside, thereby improving the mixing effect of various raw materials; S5: after the various homogenized stirring is completed, the liposome raw material is cooled, and the discharged material is inspected for standby use; the preparation of the povidone iodine solution comprises the following steps: A1: pure water, humectant, buffer, and stabilizer are added to the reactor, and the stirrer of the reactor is turned on, and the stirrer stirs the various raw materials until the various raw materials are completely dissolved; A2: the thickener is added to the reactor and continued to be stirred to ensure that the thickener is completely swollen, and then cooled to room temperature; A3: povidone iodine is added to the reactor and continued to be stirred until the povidone iodine is completely dispersed; A4: the liposome raw material and the surfactant are added to the reactor, and the stirring shaft of the stirrer continues to stir until the raw materials are evenly dispersed to obtain the povidone iodine solution of liposome encapsulation technology; A5: the povidone iodine solution obtained in step A4 is discharged from the reactor, and the discharged material is inspected and filled.

6. The method for preparing a povidone-iodine solution using liposome encapsulation technology according to any one of claim 5, characterized in that: In step S1, the heating temperature is 50-80°C; in step S2, the stirring shaft speed of the stirrer is 10-20 rpm.

7. The method for preparing a povidone-iodine solution using liposome encapsulation technology according to any one of claim 5, characterized in that: In step A1, the stirring shaft of the stirrer rotates at a speed of 10-20 rpm.

8. The method for preparing a povidone-iodine solution using liposome encapsulation technology according to any one of claim 5, characterized in that: In step A2, the stirring shaft speed of the stirrer is 10-30 rpm, and the mixture is heated to 75-85° C. during stirring for 25-35 min.

9. The method for preparing a povidone-iodine solution using liposome encapsulation technology according to any one of claim 5, characterized in that: In step A3, the stirring time is 8-12 min.

10. The method for preparing a povidone-iodine solution using liposome encapsulation technology according to any one of claim 5, characterized in that: In step A4, the stirring shaft speed of the stirrer is 20-30 rpm, and the stirring time is 8-12 min.

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