Snake oil Pickering emulsion capable of efficiently removing fishy smell as well as preparation process and application of snake oil Pickering emulsion

Abstract

The invention relates to the technical field of snake oil fishy smell removal and emulsion preparation, in particular to a snake oil Pickering emulsion capable of efficiently removing fishy smell as well as a preparation process and application of the snake oil Pickering emulsion. According to the preparation method, the snake oil is subjected to deodorization treatment by adopting a beta-molecular sieve, and the stable Pickering single emulsion loaded with the snake oil is constructed by utilizing OSAS (Octenyl Succinate) / SC (Sodium caseinate) compound particles. The Pickering emulsion shows excellent performance in the aspects of pH, ionic strength, thermal stability, centrifugal stability and the like, a new carrier is provided for delivery of fat-soluble functional components, the snake oil deodorization technology is high in practicability and wide in application prospect, and diversified application of snake oil in the fields of food, medicine and cosmetics is expected to be increased through implementation of a project.

Description

Technical Field

[0001] The present invention relates to the technical field of snake oil deodorization and emulsion preparation, and particularly relates to a highly deodorized snake oil Pickering emulsion, its preparation process and application. Background Art

[0002] The main component of snake oil is fatty acids, among which the content of unsaturated fatty acids is relatively high. In particular, eicosatetraenoic acid is significantly increased compared with warm-blooded animals, and is higher than the content in fish and amphibians (7%-9%). It has excellent compatibility and complementarity with the physiological growth characteristics of human skin, has strong affinity and permeability to skin mucosa, is easily absorbed by the skin, and is suitable for making beauty and skin care cosmetics. However, snake oil is extremely easy to oxidize and not easy to preserve, and has high viscosity and strong fishy smell, which limits its application. In addition, the snake oil content in the cream-like snake oil products used on the market is low, and flavors are mostly used to cover up the fishy smell of snake oil, further weakening the efficacy of snake oil and increasing the cost.

[0003] Pickering emulsion, also called Pickering emulsion, is a new type of emulsion system that uses solid particles to replace surfactants or amphiphilic polymers to stabilize the oil-water interface. Its stabilization mechanism is to form a stable interfacial film on the surface of the dispersed-phase droplets by solid particles, and effectively prevent the coalescence of emulsion droplets by forming steric hindrance at the oil-water interface. Compared with the emulsion formed by traditional surfactants as stabilizers, Pickering emulsion has low emulsifier dosage, high biocompatibility, and basically no irritation and toxicity. In addition, the interfacial stabilization effect of Pickering emulsion can effectively inhibit lipid oxidation and reduce the contact between unsaturated lipids and pro-oxidants and free radicals in the aqueous phase, thus weakening the oxidation reaction. Pickering emulsion has many advantages, such as encapsulating and transporting flavor substances and active substances, and delaying their degradation rate. By encapsulating flavor substances in Pickering emulsion, their bad smell can be effectively covered up, and the sensory properties of food can be improved, which has great application potential in industries such as cosmetics, food, and medicine.

[0004] Starch is one of the most commonly used polysaccharides for stabilizing Pickering emulsions. It is biocompatible and biodegradable, inexpensive, edible, non-irritating and non-toxic. Among a series of starch derivatives, octenyl succinate starch ester (OSAS) has become increasingly popular among scholars due to its strong emulsifying ability. OSAS is the product of the esterification reaction between the hydroxyl groups in the starch molecule and octenyl succinic anhydride (OSA). The introduced hydrophobic octenyl group and hydrophilic carboxyl group give OSAS amphiphilicity. The multi-branched polymer structure and high molar mass of OSAS also contribute to its adsorption at the oil-water interface to form a spatial barrier. However, since the esterification reaction mainly occurs on the particle surface, the internal hydrophobic chain is difficult to contact the oil phase when stabilizing the interface, which reduces the emulsification efficiency of OSAS. The strong rigidity and large size of OSAS also limit its continuous adsorption at the interface, which is not conducive to the long-term stability of the emulsion.

[0005] Therefore, if What Efficiently removing the smell of snake oil and constructing a stable Pickering emulsion are still technical problems that need to be solved urgently in this field. Summary of the invention

[0006] The technical problem to be solved by the present invention is: how to develop an efficient process for removing the smell of snake oil and construct a stable snake oil system based on Pickering emulsion, so as to break through the application bottleneck of snake oil in the field of medicine and health care and expand the diversified application scenarios of snake oil in the fields of food, medicine and cosmetics. Therefore, the present invention provides a Pickering emulsion for removing the smell of snake oil and its preparation process and application.

[0007] The present invention solves the above technical problems through the following technical solutions: The invention provides a preparation process of an efficient deodorized snake oil Pickering emulsion. The process comprises the following steps: using a beta-molecular sieve to deodorize the snake oil, and using octenyl succinate starch ester (OSAS) / sodium caseinate (SC) composite particles to construct a stable snake oil-loaded Pickering single emulsion.

[0008] The present invention uses β-molecular sieve to remove the fishy smell of snake oil, thereby achieving efficient fishy smell removal of snake oil, and constructs a stable Pickering single emulsion loaded with snake oil through OSAS / SC composite particles. By embedding snake oil in the Pickering emulsion, lipid oxidation can be effectively reduced.

[0009] As one of the preferred embodiments of the present invention, the snake oil is selected from any one of the snake oils of Bungarus fasciatus, Garcinia humilis, Agkistrodon acutus, Cobra and Elaphe dione, and is more preferably Bungarus fasciatus oil.

[0010] As one of the preferred embodiments of the present invention, in the step of removing the fishy smell of snake oil using octyl β-zeolite, the mass ratio of snake oil to β-zeolite is 15:1 - 5:1, more preferably 10:1.

[0011] As one of the preferred embodiments of the present invention, in the step of removing the fishy smell of snake oil using octyl β-zeolite, snake oil and β-zeolite are stirred and reacted at 30 - 50 °C for 0.5 - 5 h; more preferably, they are stirred and reacted at 35 °C for 1 h.

[0012] As one of the preferred embodiments of the present invention, for the deodorized snake oil, its optimal performance indicators are as follows: acid value 0.84 ± 0.067 mg / g, peroxide value 0.080 ± 0.004 g / 100g, conjugated diene value 2.613 ± 0.006 gL / 100g, conjugated triene value 0.8843 ± 0.001 gL / 100g. For the sample deodorized using β-zeolite, the content of alcohol volatile substances is 0.21 mg / kg, the content of other substances is 0.36 mg / kg, the content of ketone, acid, and ester substances is not higher than 0.05 mg / kg, and the hydrocarbon substance composition is similar to that of the original snake oil, reaching 1.11 mg / kg.

[0013] As one of the preferred embodiments of the present invention, the preparation method of the OSAS / SC composite particles is as follows: equal volumes of OSAS solution and SC solution are mixed at a constant speed to obtain a composite solution, and the composite solution is centrifuged and freeze-dried to obtain OSAS / SC composite particles; More preferably, in the composite solution, the composite ratio of OSAS to SC is 1:1 - 15:1, more preferably 1:1; And / or, in the composite solution, the concentration of OSAS is 6% (w / v); And / or, the degree of substitution of OSA starch is 0.024, 0.03, 0.037, more preferably 0.037; And / or, the pH of the composite solution is 4 - 5.

[0014] More preferably, during the preparation of the OSAS / SC composite particles, the constant speed mixing speed is 20 - 40 rpm; And / or, the centrifugation conditions are 3,000 g, 5 min; And / or, the freeze-drying conditions are -40 °C, 0.9 Pa.

[0015] As one of the preferred embodiments of the present invention, the particle size of the OSAS / SC composite particles is 107.07 ± 3.10 nm, the PDI is 0.52 ± 0.03, and the potential is -10.17 ± 1.80 mV.

[0016] As one of the preferred embodiments of the present invention, an aqueous solution of OSAS / SC composite particles is used as the aqueous phase, and deodorized snake oil is used as the oil phase. After mixing the aqueous phase and the oil phase, a primary emulsion is prepared by shear stirring at a certain temperature, and then a snake oil Pickering emulsion is obtained by ultrasonic treatment; More preferably, in the aqueous phase, the concentration of OSAS / SC composite particles is 2.0 wt%-10.0 wt%, more preferably 8.0 wt%; And / or, the pH of the aqueous phase is 4-5, more preferably 4; And / or, the volume fraction of the oil phase is 50.0-85.0%, more preferably 75%; And / or, the temperature is 10-25 °C; And / or, the condition parameters of shear stirring are 12000 r / min-15000 r / min; And / or, the ultrasonic power is 200-300 W, more preferably 250 W; the ultrasonic condition is ultrasonic for 6 s and stop for 3 s, and ultrasonic for a total of 5 min.

[0017] As one of the preferred embodiments of the present invention, for the snake oil Pickering emulsion, its optimal performance indexes are: emulsion particle size 22.18 ± 2.47 μm, Zeta potential 10.40 ± 0.61 mV, centrifugal water holding rate ≥60%, and it can withstand pH (2-8), ionic strength (0-200 mmoL / L NaCl) and high temperature (90 °C) environment.

[0018] The present invention also provides a highly deodorized snake oil Pickering emulsion prepared by any of the above preparation methods.

[0019] The present invention also provides an application of a highly deodorized snake oil Pickering emulsion prepared by any of the above preparation methods in the fields of cosmetics, food, healthcare products, etc.

[0020] In the present invention, sodium caseinate (SC) is a salt of casein. Casein is a safe and harmless thickening agent and emulsifying stabilizer, and has good viscosity and protein-specific foaming and gas-retaining properties. However, in practical applications, sodium caseinate usually needs to be combined with a variety of traditional emulsifiers to jointly maintain the stability of the emulsion. By compounding SC with OSAS and preparing a Pickering emulsion with deodorized snake oil, the present invention can successfully improve the stability of the snake oil emulsion.

[0021] The present invention has the following advantages compared with the existing snake oil deodorization technology: The present invention uses OSA starch / sodium caseinate composite particles as stabilizers to successfully construct a stable Pickering emulsion loaded with snake oil. By optimizing the oil phase volume fraction and particle concentration, an emulsion with a smaller particle size and a uniform microstructure is obtained. This emulsion exhibits excellent performance in terms of pH, ionic strength, thermal stability, and centrifugal stability, has a wide range of application prospects, provides a new carrier for the delivery of fat-soluble functional components of snake oil Pickering emulsion, and also expands the diversified application scenarios of snake oil in the fields of food, medicine, and cosmetics. Description of the Drawings

[0022] Figure 1 It is the XPS characterization results of β-molecular sieve before and after deodorization in Example 1.

[0023] Figure 2 It is the XRD pattern of β-molecular sieve before and after deodorization in Example 1.

[0024] Figure 3 It is the FT-IR diagram of β-molecular sieve before and after deodorization in Example 1.

[0025] Figure 4 It is a schematic diagram of the wettability of OSAS / SC composite solution under different compound ratios / pH values / substitution degrees in Example 2.

[0026] Figure 5 It is the particle size potential diagram of the emulsions obtained under the preparation conditions with an oil phase volume of 30% and 75% respectively, taking the stability ability of Pickering emulsions with snake oil as the oil phase using different composite particles as emulsifiers as an index in Example 3.

[0027] Figure 6 It is the appearance diagram of the emulsions obtained under the preparation conditions with an oil phase volume of 30% and 75% respectively, taking the stability ability of Pickering emulsions with snake oil as the oil phase using different composite particles as emulsifiers as an index in Example 3.

[0028] Figure 7 It is a schematic diagram of the preparation conditions of the composite using the composite conditions of DS = 0.037 of OSAS, a compound ratio of 1:1, and a pH value of 4 as the preparation conditions of the composite in Example 4, and the characterization of the composite prepared under this condition.

[0029] Figure 8 It is a schematic diagram of the appearance of Pickering emulsions stabilized by OSAS / SC composites at different oil phase volume fractions at different storage times in Example 5.

[0030] Figure 9It is the particle size and potential diagram of the Pickering emulsion stabilized by the OSAS / SC complex at different oil phase volumes in Example 5.

[0031] Figure 10 It is the rheological property diagram of the Pickering emulsion stabilized by the OSAS / SC complex at different oil phase volume fractions in Example 5.

[0032] Figure 11 It is the schematic diagram of the appearance of the Pickering emulsion stabilized by the OSAS / SC complex at different particle concentrations in Example 6 at different storage times.

[0033] Figure 12 It is the rheological property diagram of the Pickering emulsion stabilized by the OSAS / SC complex at different particle concentrations in Example 6.

[0034] Figure 13 It is the optical microscope micrograph of the dispersion behavior of the Pickering emulsion stabilized by the OSAS / SC complex in different dissociating agents in Example 7.

[0035] Figure 14 It is the particle size and Zeta potential diagram of the Pickering emulsion stabilized by the OSAS / SC complex at different pH values / ionic strengths in Example 8.

[0036] Figure 15 It is the schematic diagram of the appearance of the Pickering emulsion stabilized by the OSAS / SC complex at different pH values / ionic concentrations in Example 8.

[0037] Figure 16 It is the schematic diagram of the particle size and Zeta potential of the Pickering emulsion stabilized by the OSAS / SC complex at different temperatures in Example 9.

[0038] Figure 17 It is the appearance diagram of the Pickering emulsion stabilized by the OSAS / SC complex at different temperatures in Example 9.

[0039] Figure 18 It is the schematic diagram of the centrifugal stability of the Pickering emulsion stabilized by the OSAS / SC complex at different particle concentrations in Example 10.

[0040] Figure 19 It is the diagram of the in vitro antioxidant results of the Pickering emulsion in Example 11. Detailed implementation manners

[0041] In order to better clarify and understand the purpose, process scheme and advantages of the present invention, the technical scheme and implementation method of the present invention are further described clearly, completely and in detail by specific examples and in combination with the accompanying drawings. It should be noted that the embodiments described in the present invention are implemented on the premise of the technical scheme of the present invention, and detailed implementation methods and specific operation processes are given, but they are only part of the embodiments of the present invention, not all of the embodiments. The specific implementation methods described are limited to explaining and interpreting the present invention, and do not limit the present invention. Based on the embodiments in the present invention, all other implementation methods obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention. The experimental methods and conditions used in the embodiments of the present invention are conventional methods and conventional conditions unless otherwise specified. The materials, reagents or instruments, devices, etc. used in the embodiments, unless otherwise specified, are conventional substances or equipment known to those skilled in the art and can be obtained from commercial channels or prepared by conventional methods. The reaction conditions embodied in the content of the present invention can all achieve the reaction and obtain the product with the expected effect. Due to space limitations, some embodiments are listed below to further illustrate the advantages of the technical scheme of the present invention.

[0042] Example 1

[0043] This embodiment uses β-molecular sieve to remove the fishy smell of Bungarus serpentis oil purchased and boiled from a snake farm.

[0044] Snake oil:β-molecular sieve = 10:1 (w / w), react at 35°C with stirring for 1 h, stop the reaction, and centrifuge.

[0045] Physical and chemical index detection: The relative density is determined according to the relative density determination method 0601 of the fourth general rule of the 2020 edition of the Pharmacopoeia of the People's Republic of China; the acid value and saponification value are determined according to the fat and fatty oil determination method 0713 of the fourth general rule of the 2020 edition of the Pharmacopoeia of the People's Republic of China. Determination of conjugated diene value (CD) and conjugated triene value (CT): Accurately weigh 0.25 g of oil sample in a 25 mL volumetric flask, add isooctane to dilute and dissolve and make up to volume, use isooctane as a blank, and measure the absorbance A at 232 nm and 270 nm. Calculate according to the following formula:

[0046] Where: A is the absorbance of the sample at 232 nm and 270 nm; m is the sample mass / g; l is the light path of the cuvette / cm.

[0047] Analysis of fatty acid composition: Weigh 0.2 g of the homogeneous sample (accurate to 0.1 mg, containing about 100 mg - 200 mg of fat) and transfer it into a 50 mL flat-bottom flask. Add 8 mL of 0.5 mol / L sodium hydroxide methanol solution, connect the reflux condenser, and reflux on a water bath at 80°C ± 1°C until the oil droplets disappear. Add 10 mL of 14% boron trifluoride methanol solution from the upper end of the condenser, continue to reflux in a water bath at 80°C ± 1°C for 2 min, cool, accurately add 8 mL of n-hexane, continue to reflux for 1 min, then add 20 mL of saturated sodium chloride aqueous solution, and let it stand for stratification. Pipette 5 mL of the upper n-hexane extraction solution into a 25 mL test tube, add 3 g - 5 g of anhydrous sodium sulfate, shake for 1 min and then let it stand for 5 min. Inject 1 μL of the upper layer test solution into the gas chromatograph; GC-MS conditions: GC conditions: The chromatographic column is HP-FFAP (30 m × 0.25 mm × 0.25 μm); Temperature programming: The initial temperature is 70°C, maintained for 2 min, programmed to rise at a rate of 4 °C / min to 240°C, and maintained for 24 min; Injection port: 220°C; Carrier gas: Helium; Gas flow rate: 1.0 mL / min. MS conditions: Electron impact ionization source (EI); Ion source temperature: 250°C, interface temperature 240°C; Solvent delay 4 min; Scanning mode is full scan; Mass spectrometry scanning range: m / z 33 - 450. Record the chromatographic peaks and calculate the content of each fatty acid methyl ester by peak area normalization method.

[0048] Analysis of volatile components: Add 1 mL of 0.5 μg / mL 2-ethyl-1-hexanol to 4.5 g of the sample as an internal standard and equilibrate at 80°C for 10 minutes. Extract and adsorb with a 65 μm DVB / PDMS solid-phase microextraction head for 20 min and desorb for 2 min; GC-MS conditions: GC conditions: The chromatographic column is DB-5MS (30 m × 0.25 mm × 0.25 μm), injection volume 1 mL, split ratio 20:1, injection port temperature 250°C, temperature programming: The initial temperature is 35°C and maintained for 3 min, heated to 100°C at a rate of 10 °C / min, maintained for 0.5 min, heated to 250°C at a rate of 5 °C / min, and maintained for 2 min; MS conditions: Ion source EI, electron energy 70 eV, ion source temperature 200°C; Full scan mode; Mass scanning range 30 - 650 m / z; Qualitative analysis method: First remove the silicon-containing substances of column bleed, then select those with a matching degree greater than 75 (maximum 100), refer to the NIST 2020 spectral library, and combine with the mass spectrometry diagram for qualitative analysis.

[0049] FT-IR: The characteristic groups of OSA starch, SC and composite samples were determined by a Fourier transform infrared spectroscopy (FT-IR) instrument. The dry samples were mixed with KBr in a 1:100 (w / w) ratio in an agate mortar, ground and then pressed into tablets, with blank KBr as the control. The test wavelength was 4000 - 500 cm -1 , and the resolution was 4 cm -1 . The number of scans was 32 times.

[0050] XRD: The crystal structure of the composite was determined using an X-ray diffractometer (XRD). The crystal structure of the composite particles was determined by the X-ray diffractometer. The scanning speed was 2 ° / min, the diffraction angle range was set at 5 ° - 40 °, and the step size (△2θ) was set at 0.02 °.

[0051] XPS: By using a focused monochromatic Al-K source as the incident ray (hv = 1486 eV). The obtained XPS data were corrected with the binding energy of C1s (284.5 eV) as the standard energy, and the XPS data were fitted and analyzed using XPS Peak 4.1 software.

[0052] Table 1 shows the physical and chemical index results of snake oil before and after deodorization with β-molecular sieve. As can be seen from the table, the acid value of snake oil has changed significantly after deodorization, indicating that the free fatty acids in the deodorized snake oil have significantly decreased. The peroxide value, conjugated diene value and conjugated triene value of the deodorized snake oil have increased slightly, but the increase range is small, which has little impact on the quality of snake oil.

[0053] Table 1 Physical and chemical index results of snake oil before and after deodorization with β-molecular sieve

[0054] Table 2 is an analysis table of the fatty acid composition of snake oil before and after deodorization with β-molecular sieve. As can be seen from the table, the content and difference of the fatty acid composition are small after deodorizing snake oil with β-molecular sieve.

[0055] Table 2 Analysis table of the fatty acid composition of snake oil before and after deodorization with β-molecular sieve

[0056] Table 3 is the quantitative analysis table of the volatile components of snake oil before and after deodorization with β - molecular sieve. It can be seen that the total content of volatile substances in the sample after treatment with β - molecular sieve decreases significantly, especially the content of aldehyde substances shows a significant decrease. In the original snake oil sample without treatment, the total relative internal standard mass fraction of volatile components detected is 22.70 mg / kg, among which the content of aldehyde substances can reach 8.10 mg / kg. The content of aldehyde substances in the sample after treatment with β - molecular sieve decreases significantly, which is 0.45 mg / kg for β - molecular sieve treatment, 0.21 mg / kg for alcohol substances, 0.36 mg / kg for other substances, and the content of ketone, acid, and ester substances is less than 0.05 mg / kg.

[0057] Table 3 Quantitative analysis table of the volatile components of snake oil before and after deodorization with β - molecular sieve

[0058] Figure 1 is the XPS characterization result of β - molecular sieve before and after deodorization in Example 1. Figure 1 In parts A - C is the XPS spectrum of β - molecular sieve before deodorization, Figure 1 In parts D - F is the XPS spectrum sample of β - molecular sieve before deodorization. By comprehensive comparison, it may adsorb compounds containing functional groups such as amine, ketone, aldehyde, and ester on the surface. Figure 2 is the XRD spectrum of β - molecular sieve before and after deodorization in Example 1. The crystal structure of β - molecular sieve remains intact before and after deodorization without being damaged. Figure 3 is the FT - IR diagram of β - molecular sieve before and after deodorization in Example 1. The infrared characteristic peaks of the samples before and after deodorization are similar, indicating that the crystal structure of β - molecular sieve remains intact before and after deodorization without being damaged. By using means such as XRD, FT - IR, and XPS to characterize β - molecular sieve before and after deodorization, it is found that the crystal structure of β - molecular sieve remains intact before and after deodorization without being damaged. The snake oil deodorized by β - molecular sieve in this example is applied to the following examples.

[0059] Example 2

[0060] This example is to prepare OSAS / SC composite particles using octenyl succinic anhydride starch ester (OSAS) and sodium caseinate (SC).

[0061] Weigh a certain amount of OSA starch (starch substitution degree DS = 0.024, 0.03, and 0.037) and sodium caseinate and disperse them separately in deionized water. Subsequently, stir at a rate of 60 r / min for 2 h and let it stand to fully hydrate, and prepare solutions with corresponding concentrations. When in use, mix the OSA starch with SC solutions of different concentrations at a constant volume and constant speed (20 - 40 rpm) to obtain composite solutions with different OSAS / SC composite ratios (r = 1:1, 3:1, 6:1, 9:1, 12:1, 15:1, m / m). The concentration of OSA starch in the composite solution is fixed at 6% (w / v). The pH value (pH = 3 - 9) of the composite solution is adjusted with different concentrations of HCl or NaOH (0.01 mol / L - 2 mol / L) to ensure the minimum dilution effect. The OSAS / SC composite is prepared by centrifuging the composite solution (centrifugation conditions: 3000 g, 10 min) and freeze-drying (-40 °C, 0.9 Pa). The yield (%) of the composite is determined by calculating the ratio of the mass of the dried composite to the total mass of OSA starch and SC added in the initial composite solution. The freeze-dried particles of the OSAS / SC composite are used to prepare the following Pickering emulsions.

[0062] Zeta potential and average particle size determination: The Zeta-potential, average particle size, and polydispersity index (PDI) of the composite solution are measured using a Malvern Zetasizer Nano ZS90 particle size analyzer. Before measurement, the composite solution is diluted and shaken with deionized water at a ratio of 1:100 (v / v) to avoid the multiple scattering effect. During measurement, 2 mL of the sample to be measured is taken and measured at 25 °C, and each sample is measured in parallel three times.

[0063] Measurement of the three-phase contact angle of the composite particles: The three-phase contact angle of the composite is measured by a video optical contact angle measuring instrument ( θow ) for measurement and analysis. It is immersed in a glass colorimetric dish filled with soybean oil. Using the sessile drop method, 5 μL of deionized water is slowly dropped onto the surface of the cylindrical slice using a high-precision syringe. After equilibration for 4 s, the droplet image is captured using a high-speed camera, and θow and its change over time are obtained by fitting the Laplace-Young equation. Each sample is measured in parallel three times.

[0064] Data analysis: All experiments are independently repeated more than 3 times, and the results are expressed as mean ± standard deviation.

[0065] The yields, particle sizes, and Zeta - potentials of the OSAS / SC complexes are shown in Table 4. The yields under different degrees of substitution are in the order of: DS = 0.037 > DS = 0.024 > DS = 0.030. The yields under 6 compounding ratios are in the order of: 15:1 > 6:1 > 9:1 > 12:1 > 1:1 > 3:1. The yields under 7 pH values are in the order of pH 6 > pH 9 > pH 5 > pH 3 > pH 4 > pH 8 > pH 7.

[0066] Table 4 Results of the yields, particle sizes, PDI, and Zeta potentials of the OSAS / SC complexes under different compounding ratios / degrees of substitution / pH values (n = 3)

[0067] For OSA starches with different degrees of substitution θow are 34.35°, 31.10°, and 30.35° respectively, and they are extremely hydrophilic. After electrostatic complexation with SC, the complexes θow increase. Figure 4 is a schematic diagram of the wettability of the OSAS / SC complex solution under different compounding ratios / pH values / degrees of substitution in Example 2. Different compounding ratios have a greater impact on the θow OSAS / SC complexes. When the compounding ratio is 1:1, the contact angle of the complex is the largest at 63.05°. The pH value also has a certain impact on the complex θow When pH = 3, which is less than the isoelectric point of SC, SC flocculates, the SC content decreases, and the relative content of OSA increases, θow decreases. When pH = 5, the Zeta potential increases significantly and the particle size decreases significantly; when the pH further increases, both OSA starch and SC carry negative charges, and electrostatic repulsion is likely to occur, leading to an increase in particle size; when the pH is less than the isoelectric point (pI = 4.6), sodium caseinate carries a positive charge, and OSA starch is partially deprotonated at pH = 4 and has a negative charge on the surface. The electrostatic attraction between positive and negative charges between OSA starch and SC drives the combination of the two to form a complex; when the pH further decreases, the charge intensity weakens, the number of positive and negative charges in the solution is similar, the electrostatic repulsion between SC decreases, and flocculation occurs, resulting in a decrease in yield. As the mass concentration of OSA starch increases, the negative charge number of the Zeta potential of the OSAS / SC composite solution shows a decreasing trend in different amplitudes. At the same time, the PDI of the OSAS / SC solution gradually increases, and the particle size distribution becomes more uneven. When the compounding ratio of OSA starch to SC reaches 1:1, its particle size is the smallest, reaching 197.57 ± 3.17 nm, and the absolute value of the Zeta potential is the largest at - 35.17 ± 2.45 mV.

[0068] Combined with the particle size, potential, and contact angle results of OSAS / SC complexes under the above different compounding ratios / pH values / degree of substitution, the compounding conditions with a DS of 0.037 for OSA starch, a compounding ratio of 1:1, and pH values of 4 and 5 were selected as the preparation conditions for further screening of the complexes.

[0069] Example 3

[0070] In this example, the method of Example 2 was used to prepare OSAS / SC complex particles (pH = 4, 5). The preparation principle was to characterize the particle size and potential of different complex particles (pH = 4, 5) and use their ability to stabilize Pickering emulsions with snake oil as the oil phase as an index. The preparation conditions with oil phase volumes Φ of 30% and 75% were selected respectively. By characterizing the particle size, potential, and appearance structure of the obtained emulsions, the two complexes were further screened. Table 5 shows the yield, particle size, PDI, and Zeta potential results of OSAS / SC complexes (pH = 4, 5). When the pH of the OSAS / SC complex was 4, the yield reached 33.13 ± 4.23%, the minimum particle size reached 107.07 ± 3.10 nm, the PDI was 0.52 ± 0.03, and the Zeta potential reached -10.17 ± 1.80 mV.

[0071] Table 5 Yield, particle size, PDI, and Zeta potential results of OSAS / SC complexes (pH = 4, 5) in Example 3

[0072] The particle size and potential results of Pickering emulsions are as Figure 5 shown. When pH = 4 and Φ = 75%, the particle size of the emulsion was the smallest; the absolute value of the Zeta potential of the two emulsions prepared using the composite particles OSAS / SC (pH = 5) was significantly higher than that of the emulsions prepared using the composite particles OSAS / SC (pH = 4), reaching -28.6 ± 0.3 mV, and the result was related to the Zeta potential of the complex particle solution. Attached Figure 6 is the appearance diagram of the 4 emulsions in this example after standing for 24 h. In the figure, A - OSAS / SC complex (pH = 4) (Φ = 30%); B - OSAS / SC complex (pH = 4) (Φ = 75%); C - OSAS / SC complex (pH = 5) (Φ = 30%); D - OSAS / SC complex (pH = 5) (Φ = 75%). It can be clearly found that the two emulsions prepared using the composite particles OSAS / SC (pH = 5) showed stratification and oil phase precipitation, and the emulsion stability was poor. When pH = 4 and Φ = 75%, the minimum particle size of the emulsion could reach 79.36 ± 1.97 μm, and the emulsion had good stability and did not stratify after standing at room temperature for 24 h.

[0073] Combined with the above results, the substitution degree of OSA starch was finally selected as 0.037, the compound ratio was 1:1, and the pH value was 4 as the preparation conditions of the complex. The complex prepared under these conditions was used as an emulsifier to stabilize the Pickering emulsion with snake oil as the oil phase.

[0074] Example 4

[0075] In this example, the OSAS / SC composite particles (substitution degree DS = 0.037, pH = 4, compound ratio 1:1) were used as the preparation conditions of the complex, and they were characterized.

[0076] Determination of characteristic groups and crystal structure of composite particles: The characteristic groups of OSA starch, SC and composite samples were determined by a Fourier transform infrared spectroscopy (FT-IR) instrument. The dry samples and KBr were mixed and ground in an agate mortar at a ratio of 1:100 (w / w), and then pressed into tablets, with blank KBr as the control. The test wavelength was 4000 - 500 cm -1 , the resolution was 4 cm -1 , and the number of scans was 32 times. The crystal structure of the complex was determined by an X-ray diffractometer (XRD). The composite particle powder was equilibrated in a dryer containing saturated sodium chloride solution for one week, and the crystal structure of the composite particles was determined by the X-ray diffractometer. The scanning speed was 2 ° / min, the diffraction angle range was set from 5° to 40°, and the step size (△2θ) was set to 0.02°.

[0077] Observation of the microscopic morphology of composite particles: The surface morphology of the composite was observed by a scanning electron microscope (SEM). The sample was fixed with conductive glue, and a gold coating with a thickness less than 10 nm was sprayed on its surface, and then characterized and analyzed using a scanning electron microscope, with a magnification of 2000.

[0078] The results of the characteristic groups and crystal structure of the composite are as Figure 7 shown. Appendix Figure 7 Part A is the FT-IR diagram of the composite, and the results show that sodium caseinate and OSA starch interacted to form a relatively stable OSAS / SC binary complex, rather than a simple physical mixture. Appendix Figure 7 Part B is the XRD pattern of OSA, SC and the OSAS / SC composite. It can be seen from the figure that an amorphous complex with intermolecular interaction was formed between OSA and SC in the composite, which is consistent with the above FT-IR results.

[0079] Appendix Figure 7The CE parts in the middle are the SEM results of OSAS, SC and OSAS / SC composites. The morphological and structural characteristics of the samples were observed by SEM. In the SEM images of OSAS, SC and OSAS / SC composites, OSAS showed an uneven spherical shape with micropores inside; SC was an irregular particle with obvious wrinkles on the surface; both OSAS / SC composite particles showed a lamellar network structure, in which the lamellar layers were connected by micropores, proving that OSAS and SC interacted with each other.

[0080] Example 5

[0081] This example is OSAS / SC composite particles (substitution degree DS=0.037, pH=4, composite ratio 1:1), and the method for stably loading snake oil is to screen the appearance and particle size of the snake oil Pickering emulsion by the oil phase volume fraction, and explore the effect of the oil phase volume fraction on the formation of Pickering emulsion.

[0082] Preparation of Pickering emulsion stabilized by OSA starch / SC composite particles: The OSAS / SC complex was prepared by the method in Example 2, and the OSAS / SC complex solution was obtained by lyophilization and dilution with deionized water, and the pH value was adjusted to 4. The complex solution was mixed with snake oil, and sheared and stirred at 12000 r / min for 3 min at room temperature to prepare a primary emulsion. Under the condition of ultrasonic power of 250 W, the primary emulsion was ultrasonicated for 5 min, the ultrasound was turned on for 6 s, and turned off for 3 s to obtain a snake oil Pickering emulsion. When investigating the effect of the oil phase volume fraction (Φ=5.0%-85.0%, v / v) on the emulsion, c was fixed at 3.0 wt%, and the total volume of the emulsion was set to 60 mL.

[0083] In the Pickering emulsion prepared in this study, during the emulsification process, the OSAS / SC complex was adsorbed from the aqueous phase to the surface of fat globules, preventing the aggregation of small fat globules and the generation of large fat globules, thereby maintaining the stability of the emulsion. Figure 9 : is the particle size potential diagram of the Pickering emulsion stabilized by the OSAS / SC complex at different oil phase volumes in Example 5. Different oil phase volumes have an effect on the average particle size D of the emulsion [4,3] It has a greater impact. When Φ≥75%, the emulsion particle size is significantly reduced, which is related to the high oil phase ratio causing the emulsion to transform from O / W type to W / O type emulsion.

[0084] Attached Figure 8This is the appearance diagram of the Pickering emulsion stabilized by the OSA / SC composite at different oil phase volume fractions in this example. In the figure, A is the appearance diagram of the Pickering emulsion stabilized by the OSA / SC composite at different oil phase volume fractions (stored for 3 days); B is the appearance diagram of the Pickering emulsion stabilized by the OSA / SC composite at different oil phase volume fractions (stored for 5 days); C is the appearance diagram of the Pickering emulsion stabilized by the OSA / SC composite at different oil phase volume fractions (stored for 7 days); D is the appearance diagram of the Pickering emulsion stabilized by the OSA / SC composite at different oil phase volume fractions (stored for 15 days). From the appearance diagrams of the Pickering emulsion stabilized by the OSAS / SC composite at different storage times under different oil phase volume fractions, it can be found that when Φ = 75%, the emulsion remains stable, and no cream separation phenomenon occurs after 2 weeks of storage. Figure 9 is the particle size potential diagram of the Pickering emulsion stabilized by the OSAS / SC composite at different oil phase volumes in Example 5. Different oil phase volumes have a greater impact on the average particle size D of the emulsion [4,3] When Φ ≥ 75%, the particle size of the emulsion decreases significantly, which is related to the fact that a high oil phase ratio causes the emulsion to change from an O / W type to a W / O type emulsion. Figure 10 is the rheological property diagram of the Pickering emulsion stabilized by the OSAS / SC composite at different oil phase volume fractions in Example 5. Except for Φ = 75%, as the shear frequency increases, the emulsion gradually evolves from a weak elastic structure (G′ > G′′) to a viscous fluid (G′ < G′′), indicating that the internal gel structure of the emulsion is unstable. In summary, Φ = 75% is selected as the optimal oil phase volume.

[0085] Example 6

[0086] In this example, the OSAS / SC composite particles (degree of substitution DS = 0.037, pH = 4, composite ratio 1:1) are used. The method for stably loading snake oil is to screen the appearance and particle size of the snake oil Pickering emulsion through the concentration of the OSAS / SC composite particles. When investigating the influence of the composite particle concentration (c = 0.2% - 10.0%, wt%) on the emulsion, Φ is fixed at 40%, and the total volume of the emulsion is set to 60 mL.

[0087] Appendix Figure 11This is the appearance diagram of Pickering emulsions stabilized by OSA / SC complexes at different particle concentrations in this example. In the figure, A - Appearance diagram of Pickering emulsions stabilized by OSA / SC complexes at different particle concentrations (stored for 3 days); B - Appearance diagram of Pickering emulsions stabilized by OSA / SC complexes at different particle concentrations (stored for 5 days); C - Appearance diagram of Pickering emulsions stabilized by OSA / SC complexes at different particle concentrations (stored for 7 days); D - Appearance diagram of Pickering emulsions stabilized by OSA / SC complexes at different particle concentrations (stored for 15 days). Table 6 shows the particle size and zeta-potential results of Pickering emulsions stabilized by OSAS / SC complexes at different particle concentrations in Example 6.

[0088] Table 6 Particle size and zeta-potential results of Pickering emulsions stabilized by OSAS / SC complexes at different particle concentrations

[0089] The results show that the stability of the emulsion increases with the increase in particle concentration. By investigating the particle size, Zeta-potential, and appearance morphology of Pickering emulsions stabilized by OSAS / SC complexes at different particle concentrations, it was found that the particle size of the Pickering emulsion stabilized by 0.2% OSAS / SC complex was 32.67 ± 4.90 μm. When the particle concentration was further increased to 0.5 wt%, the particle size of the emulsion droplets decreased to 3.26 ± 0.08 μm. With the further increase of c, the oil droplet size became smaller. The rheological property results of Pickering emulsions at different particle concentrations are shown in Figure 12 . All emulsions showed similar shear-thinning behavior, and the apparent viscosity and viscoelastic moduli (G′ and G′′) were positively correlated with the complex particle concentration. However, all emulsions showed a viscous fluid structure (G′ < G′′), and the internal gel structure of the emulsion was unstable. Further research was carried out on Pickering emulsions with an oil phase volume of 75% at different particle concentrations. When c = 8%, the G′ of the emulsion was much higher than its corresponding G′′, and the two moduli were hardly affected by the shear frequency, indicating that the emulsion formed a highly elastic gel network structure. Combining the rheological properties of Pickering emulsions at different particle concentrations and the emulsion stability at different storage times, the particle concentration of c = 8% was finally selected as the optimal concentration for emulsion preparation.

[0090] Example 7

[0091] This example examines the effect of the dispersion of OSAS / SC complex-stabilized Pickering emulsions in different dissociating agents. After mixing the emulsion with different dissociating agents, including deionized water, urea (6 mol / L), sodium chloride (100 mmol / L), and SDS (1%, w / v), at a ratio of 1:9 (w / w), the mixture was allowed to stand at 25 °C for 1 h, and then the dispersion of the emulsion was observed. Subsequently, it was vortexed at low speed for 20 s, and the microstructure of the emulsion was observed using an optical microscope.

[0092] Figure 13 It is a microscopic schematic diagram of the optical microscope of the dispersion behavior of OSAS / SC complex-stabilized Pickering emulsions in different dissociating agents in Example 7. By exploring the interaction force and flocculation structure between the droplets of OSAS / SC complex-stabilized Pickering emulsions with different dissociating agents, it can be obtained that the flocculation state of the emulsion droplets after dilution with NaCl changes significantly, indicating that the interaction degree between the droplets through electrostatic interaction is relatively high on the basis of flocculation.

[0093] Example 8

[0094] This example is used to detect the effects of pH and ionic strength on OSAS / SC complex-stabilized Pickering emulsions.

[0095] To investigate the effect of pH on the stability of Pickering emulsions, the pH of the Pickering emulsions was adjusted to 3.0, 4.0, 5.0, 6.0, 7.0, and 9.0 using 1 mol / L HCl solution or NaOH solution, respectively. After refrigerating at 4 °C for 5 h, the appearance, droplet size, and Zeta potential of the emulsion were recorded.

[0096] Figure 14 It is a graph of the particle size and Zeta potential of OSAS / SC complex-stabilized Pickering emulsions at different pH values / ionic strengths in Example 8. Figure 15It is a schematic diagram of the appearance of Pickering emulsions stabilized by OSAS / SC complexes at different pH values / ion concentrations in Example 8. The results show that the Pickering emulsions stabilized by OSAS / SC complexes have a certain responsiveness to pH changes and certain ionic stability. When the pH value of the emulsion ranges from 3.0 to 5.0, as the potential further decreases from -1.2 mV to -21.67 mV, the repulsive force between the droplets increases, the stability of the emulsion increases, and the droplet size decreases significantly. The droplet size of the Pickering emulsion decreases from 390.67 μm to 1.38 μm. When the pH value further increases, the droplet size of the Pickering emulsion significantly increases; when pH = 5.0 and 6.0, the emulsion remains stable and presents a uniform and stable appearance; when pH ≥ 7, both OSA starch and SC carry negative charges, and the excessive electrostatic repulsive force may cause the complex structure to become loose and the emulsion stability to decrease. This result shows that the Pickering emulsions stabilized by OSAS / SC complexes have a certain responsiveness to pH changes.

[0097] To investigate the effect of ionic strength on the stability of Pickering emulsions, different concentrations of NaCl (0 mmol / L, 30 mmol / L, 60 mmol / L, 90 mmol / L, 120 mmol / L, 150 mmol / L, and 200 mmol / L) were mixed with the Pickering emulsions at a ratio of 1:1 (V / V), and after refrigerating at 4 °C for 24 h, the appearance, droplet size, and Zeta potential of the emulsions were recorded. Figure 14 Part B shows the changes in the droplet size and potential of the emulsions at different ionic strengths. When the NaCl concentration increases from 0 mmol / L to 200 mmol / L, the droplet size of the emulsion increases slowly. When the NaCl concentration reaches 120 mmol / L, the increase in ionic concentration has little effect on the droplet size and potential of the emulsion. This result is related to the shielding effect of salt ions on the surface potential of the complex and droplets. As the NaCl concentration continuously increases, the potential of the emulsion droplets decreases, the electrostatic repulsive force between the droplets weakens, and partial coalescence of the emulsion droplets occurs, resulting in an increase in droplet size. However, when the ionic concentration further increases, the effect on the droplet size and potential of the emulsion is smaller.

[0098] Example 9

[0099] This example is used to test the effect of different temperatures on the Pickering emulsions stabilized by OSAS / SC complexes.

[0100] By storing the Pickering emulsion of Example 5 (c = 8%, Φ = 75%) at different temperatures (-4 °C, 25 °C, 37 °C, 60 °C, and 90 °C) for 30 min, the effect of temperature on the emulsion stability was explored.

[0101] Figure 16 It is a schematic diagram of the particle size and Zeta potential of Pickering emulsions stabilized by OSAS / SC complexes at different temperatures in Example 9. Figure 17 It is a picture of the appearance of Pickering emulsions stabilized by OSAS / SC complexes at different temperatures in Example 9. There was no obvious change in the appearance of the Pickering emulsions stored at different temperatures, and no demulsification occurred. This result indicates that the Pickering emulsions stabilized by OSAS / SC complexes have good temperature flexibility.

[0102] Example 10

[0103] In this example, the centrifugal stability of Pickering emulsions stabilized by OSAS / SC complexes at different particle concentrations (c = 3 - 10%, Φ = 75%) in Example 6 was detected. 15 g of freshly prepared Pickering emulsion was placed in a 50 mL centrifuge tube and centrifuged at 4000 g for 15 min at 4°C. The change in the appearance of the emulsion after centrifugation was used as the basis for judging its centrifugal stability. At the same time, the centrifugal stability of the emulsion was further analyzed in combination with WHC. After centrifugation, the upper oil phase, the lower water phase, and the middle emulsion were weighed respectively, and the WHC of the emulsion was calculated according to the following formula:

[0104] W in the formula T is the total mass of the sample, and W F is the mass of the water released after centrifugation.

[0105] Centrifugation accelerates the creaming process of the emulsion and forces the droplets to aggregate. After centrifugation, excess water is discharged from the emulsion, creating a tight encapsulation condition. Phase separation occurred in all the emulsions after centrifugation. The lower layer of the centrifuge tube was water, and the upper layer was the emulsion after water separation, but no oil leakage caused by demulsification was found. Figure 18 Part A of is a schematic diagram of the centrifugal stability of Pickering emulsions stabilized by OSAS / SC complexes at different particle concentrations in Example 10, Figure 18 Part B of is a graph of the water-holding capacity of Pickering emulsions stabilized by OSAS / SC complexes at different particle concentrations in Example 10. The water-holding capacity of all emulsions reached more than 60%, and the stable structure of the emulsion could retain more water.

[0106] Example 11

[0107] This example detects the in vitro antioxidant activity of the Pickering emulsion ((c = 8%, Φ = 75%)) in Example 5. Using a DPPH free radical scavenging ability kit, prepare each test solution and working solution according to the requirements of the test kit. The sample was diluted 4-fold with absolute ethanol. The control group was 400 μL of the sample added to 600 μL of absolute ethanol; the measurement group was 400 μL of the sample added to 600 μL of the DPPH working solution; the blank group was 400 μL of absolute ethanol added to 600 μL of the DPPH working solution, and mixed well. Leave it to stand in the dark at room temperature (25 °C) for 30 min, centrifuge at 4000 r / min for 5 min, take the supernatant, measure its OD value at 517 nm, and calculate the DPPH free radical scavenging rate (%) according to the following formula:

[0108] Using a DPPH free radical scavenging ability kit, prepare each test solution and working solution according to the requirements of the test kit. The sample was diluted 4-fold with absolute ethanol and 20 μL of the peroxidase working solution was added to each well. Add 10 μL of distilled water, 10 μL of standard solutions with different concentrations, and the diluted sample solution to be tested (diluted 4-fold) to the blank well, standard well, and measurement well respectively, shake gently, and add 170 μL of the ABTS working solution to each well and shake well. React at room temperature for 6 min, measure the absorbance at a wavelength of 405 nm using a microplate reader, and obtain the total antioxidant capacity of the Pickering emulsion and deodorized cobra oil using the standard curve. Figure 19 Part A is the result graph of the DPPH free radical scavenging ability of the Pickering emulsion in Example 11, Figure 19 Part B is the result graph of the total antioxidant capacity of the Pickering emulsion in Example 11. The results show that the antioxidant capacity of the Pickering emulsion is higher than that of the original snake oil. The total antioxidant capacity of the Pickering emulsion is 0.66 mM, equivalent to 0.66 mM of Trolox. The total antioxidant capacity of the original snake oil decreased slightly, being 0.45 mM of Trolox. The in vitro antioxidant experiment proves that snake oil has a certain antioxidant capacity, and its antioxidant capacity can be further improved after being prepared into a Pickering emulsion.

[0109] Through the above experiments, it was proved that β-molecular sieve was used to remove the fishy smell of snake oil, and OSAS / SC was prepared as stable particles. Pickering emulsion was prepared by ultrasonic assistance. The changes in the particle size, appearance and microstructure of the emulsion were studied at different OSA / SSC concentrations and different oil phase volume fractions. It was found that when the oil phase volume fraction was constant, when the OSAS / SC concentration increased to 8.0%, the particle size decreased to 22.18 ± 2.47 μm; when the OSAS / SC concentration was constant, as the oil phase volume fraction increased, the particle size increased and the emulsion layer became thicker. As the oil phase volume fraction increased to 75% and stored at room temperature for 15 days, the emulsion remained stable and no cream separation or demulsification and stratification occurred. The microstructure of the Pickering single emulsion stabilized by composite particles was studied by optical microscopy. It was found that the emulsion droplets were round and closely connected, and OSAS / SC was firmly adsorbed to the oil-water interface. The pH / ionic strength stability / thermal stability / centrifugal stability of the Pickering single emulsion stabilized by composite particles was explored. The results showed that the Pickering emulsion had good temperature flexibility, high stability to ionic strength and certain responsiveness to pH changes. In the centrifugal stability test, it was found that the water holding capacity of the emulsion reached more than 60%, and the stable structure of the emulsion could retain more water.

Claims

1. A process for preparing a highly effective and fishy-free snake oil Pickering emulsion, characterized in that: The process comprises the following steps: using beta-molecular sieve to remove the smell of snake oil, and using octenyl succinate starch ester OSAS / sodium caseinate SC composite particles to construct a Pickering single emulsion loaded with snake oil.

2. The preparation process of a highly effective deodorized snake oil Pickering emulsion according to claim 1, characterized in that: The snake oil is selected from any one of the snake oils of Bungarus fasciatus, Garcinia humilis, Agkistrodon acutus, Cobra and Elaphe dione.

3. The preparation process of a highly effective deodorized snake oil Pickering emulsion according to claim 1, characterized in that: In the step of removing the smell of snake oil by using β-molecular sieve, the mass ratio of snake oil to β-molecular sieve is 10:0.1-10, and the snake oil and β-molecular sieve are stirred and reacted at 30-50° C. for 0.5-5h.

4. The preparation process of a highly effective deodorized snake oil Pickering emulsion according to claim 3, characterized in that: In the step of removing the smell of snake oil by using β-molecular sieve, the mass ratio of snake oil to β-molecular sieve is 10:1, and the snake oil and β-molecular sieve are stirred and reacted at 35° C. for 1 hour.

5. The preparation process of a highly effective deodorized snake oil Pickering emulsion according to claim 1, characterized in that: The preparation method of the OSAS / SC complex particles is as follows: equal volumes of OSAS solution and SC solution are mixed at a constant speed to obtain a complex solution, and the complex solution is centrifuged and freeze-dried to obtain the OSAS / SC complex particles.

6. The preparation process of a highly effective deodorized snake oil Pickering emulsion according to claim 5, characterized in that: In the composite solution, the composite ratio of OSAS and SC is 1-15:1; and / or, in the composite solution, the concentration of OSAS is 6% (w / v); and / or, the degree of substitution of OSA starch is 0.024, 0.03, 0.037; And / or, the pH of the composite solution is 4-5.

7. The preparation process of a highly effective deodorized snake oil Pickering emulsion according to claim 1, characterized in that: The aqueous solution of OSAS / SC composite particles was used as the water phase, and the deodorized snake oil was used as the oil phase. The water phase and the oil phase were mixed, and then sheared and stirred at a certain temperature to prepare a primary emulsion, and then the snake oil Pickering emulsion was obtained by ultrasound.

8. The preparation process of a highly effective deodorized snake oil Pickering emulsion according to claim 7, characterized in that: In the aqueous phase, the concentration of OSAS / SC composite particles was 0.6 wt%-10.0wt%; and / or, the pH of the aqueous phase is 4-5; and / or, the volume fraction of the oil phase is 50.0-85.0%; And / or, the ultrasonic power is 200-300 W, more preferably 250 W; the ultrasonic condition is ultrasonic for 6 seconds and stop for 3 seconds, for a total of 5 minutes.

9. An efficient deodorized snake oil Pickering emulsion prepared according to the preparation process according to any one of claims 1 to 8.

10. Use of the highly effective and deodorized snake oil Pickering emulsion prepared by the preparation process according to any one of claims 1 to 8 in the fields of cosmetics, food, and pharmaceutical health products.

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