Soybean seed dressing aid and preparation method thereof

Abstract

The invention discloses a soybean seed dressing aid and a preparation method thereof, and mainly relates to the technical field of fertilizers. The raw materials comprise alginate oligosaccharide, a chelated molybdenum composition, boric acid or borax, a surfactant and water, and the chelated molybdenum composition is prepared by performing chelation reaction on citric acid, composite sugar alcohol and ammonium molybdate and then mixing with EDTA-Mo (Ethylene Diamine Tetraacetic Acid-Mo). The method has the beneficial effects that through an alginate oligosaccharide-boron molybdenum synergistic system, soybean seed germination can be effectively promoted, the soybean emergence speed is increased, the soybean emergence rate is increased, and soybean root development is promoted; and bad symptoms such as low emergence rate, irregular emergence and germ malformation after emergence caused by seed dressing agents or bad weather can be effectively relieved, so that the purposes of cultivating strong seedlings, improving the stress resistance of the seedlings, stabilizing the yield and improving the quality are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and specifically to a soybean seed dressing assistant and a preparation method thereof. Background Art

[0002] In the process of modern agricultural planting, leguminous crops such as soybeans and peanuts often adopt the method of seed dressing during sowing to prevent the occurrence of soil pests and diseases. However, the use of seed dressing agents has had an adverse impact on the emergence effect of leguminous crops to a certain extent, resulting in problems such as reduced crop emergence rate, uneven emergence, and deformed germ after emergence, which has seriously affected the later growth and development of crops. Moreover, in recent years, due to the frequent occurrence of extreme weather such as extreme high temperature, low temperature, hail, drought, and flood caused by global warming, the normal growth and development of crops have been affected, and in severe cases, crop production has been reduced or even the crops have failed. At present, in the process of agricultural production, increasing the crop emergence rate, cultivating strong seedlings, promoting the downward growth of crop roots, and increasing root biomass are the prerequisites for ensuring crop yields.

[0003] In the existing technical means, adding microbial inoculants, rooting powders or applying rooting fertilizers, microbial inoculants and other fertilizer products after emergence, and spraying plant regulators such as gibberellic acid and brassinolide on the leaves are the main means to prevent pests and diseases of leguminous crops, promote the rooting of soybeans, and boost seedling growth. However, the former only plays a role in preventing pests and diseases to a certain extent, and has an insignificant effect on improving the emergence rate. Moreover, unreasonable dosage will also inhibit crop emergence; while the latter is only carried out after the emergence of soybeans, and cannot effectively solve the problems of uneven emergence and deformed seedlings that have already occurred. Summary of the Invention

[0004] The purpose of the present invention is to provide a soybean seed dressing assistant and a preparation method thereof. Through the alginate oligosaccharide-boron-molybdenum synergistic system, it can not only effectively promote the germination of soybean seeds, improve the emergence speed of soybeans, increase the emergence rate of soybeans, and promote the root development of soybeans; but also effectively alleviate the adverse symptoms such as low emergence rate, uneven emergence, and deformed germ after emergence caused by seed dressing agents or bad weather, thereby achieving the purpose of cultivating strong seedlings, improving the stress resistance of seedlings, and stabilizing and improving yields.

[0005] To achieve the above object, the present invention is realized through the following technical solutions:

[0006] A soybean seed dressing assistant, the raw materials of which include alginate oligosaccharide, chelated molybdenum composition, boric acid or borax, surfactant, and water. The chelated molybdenum composition is formed by chelating citric acid, compound sugar alcohol and ammonium molybdate, and then mixing with EDTA-Mo.

[0007] The alginate oligosaccharide is produced by one-step enzymatic hydrolysis of sodium alginate using alginate lyase enzymatic hydrolysis technology, with a degree of polymerization of 2-4, a molecular weight of 800-1000 Da, and an alginate oligosaccharide content of ≥95%.

[0008] The alginate oligosaccharide is composed of at least one of sorbitol, erythritol, xylitol, lactitol and mannitol in a ratio of 2-4:1.

[0009] In the chelation reaction, the ratio of citric acid, complex sugar alcohol and ammonium molybdate is 0.1-0.15:0.25-0.45:0.9-1.2.

[0010] The reaction pH value range of the chelation reaction is 3.8-4.5, the reaction temperature is 65-75 °C, and the reaction time is 40-60 min.

[0011] The surfactant is a composition of AEO-3 and AEO-15, and the ratio is 1:1.5-2.

[0012] When the raw material is boric acid, before use, boric acid and water are first dissolved completely at a ratio of 1:8-10 at 50-60 °C, the pH is adjusted to 4.5-5.5, and then mixed and stirred evenly with the alginate oligosaccharide for standby.

[0013] It is prepared by the following method:

[0014] S1, at room temperature, add 10-20 parts of boric acid to 80-200 parts of water, heat to 50-60 °C, continuously stir for 5-10 min, after complete dissolution, adjust the pH to 4.5-5.5, add 90-100 parts of alginate oligosaccharide, and continuously stir for 10-15 min until completely dissolved to obtain solution A;

[0015] S2, at room temperature, add 15-22.5 parts of citric acid and 135-180 parts of ammonium molybdate to 260-360 parts of water, pressurize and heat to 65-75 °C, maintain this temperature and continuously stir for 20-30 min; adjust the pH to 3.8-4.5, add 37.5-67.5 parts of complex sugar alcohol, maintain this temperature and continue to stir for 20-30 min; add 10-20 parts of EDTA-Mo, continue to stir for 5-10 min and then stop heating to obtain solution B;

[0016] S3, add solution A to solution B, add 200-300 parts of water, adjust the pH to 3.8-4.8, heat to 40-50 °C, and continuously stir for 10-20 min;

[0017] S4, stop heating, add 5-10 parts of surfactant while stirring, continuously stir for 10-15 min, and then stand still and fill to obtain this seed dressing adjuvant product.

[0018] In specific applications, this seed dressing adjuvant is used after being mixed evenly with leguminous seeds at a dressing amount of 10-60 g / 100 kg, and / or is sprayed on the leaves after emergence after being diluted 1500-3000 times.

[0019] A preparation method of a soybean seed dressing adjuvant, comprising: obtaining liquid A with boric acid or borax and fuco-oligosaccharide as raw materials; after chelating citric acid, compound sugar alcohol and ammonium molybdate, and mixing with EDTA-Mo to obtain a chelated molybdenum composition, which is used as liquid B; S3, mixing liquid A, liquid B and water, and then mixing in a surfactant to obtain the product.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] This adjuvant is mainly composed of high-purity fuco-oligosaccharide, supplemented with trace elements such as boron and molybdenum, and a seed dressing adjuvant for leguminous crops is obtained through a citric acid-sugar alcohol chelation reaction. Its advantages are as follows:

[0022] First, the degree of polymerization of the fuco-oligosaccharide used is 2-4 sugars, and the molecular weight is 800-1000Da. Combining with boron element can not only rapidly promote seed germination, induce the synthesis of plant IAA, stimulate crop growth, and promote the formation of crop roots; but also enhance the stress resistance of soybeans. As a signal molecule, fuco-oligosaccharide can activate the jasmonic acid and salicylic acid signal pathways of crops, improve the ability of leguminous crops such as soybeans to cope with abiotic stresses such as phytotoxicity, drought, and cold, and make up for the deficiencies of the prior art in slow emergence, uneven emergence, and poor root development of soybeans.

[0023] Second, through the chelation reaction of citric acid, compound sugar alcohol and ammonium molybdate, and compounding with EDTA-Mo, the content of hexavalent molybdenum can be increased, and the proportion of pentavalent molybdenum content can be reduced, so that the chelated molybdenum composition system is more stable and is not likely to fail due to reactions such as flocculation and precipitation with the seed dressing agent. At the same time, using citric acid and compound sugar alcohol as chelating agents for ammonium molybdate can accelerate the transfer and distribution of molybdenum elements in the soybean seedlings after emergence, and improve the utilization efficiency of molybdenum elements by soybeans. Solve the problem of increased labor cost of foliar spraying after soybean emergence in the prior art.

[0024] Third, the fuco-oligosaccharide-boron molybdenum synergistic system has higher stability and can participate in the synthesis of nitrate reductase in crops more efficiently, thereby improving the absorption, conversion and protein synthesis efficiency of nitrogen elements by crops, promoting the formation and development of root nodules of leguminous crops, enhancing the nitrogen fixation ability of crops, and strengthening the plants.

[0025] Fourth, compared with existing products, this seed dressing adjuvant is more convenient, safe and efficient for seed dressing. When used together with the seed dressing agent, no additional water is required, which simplifies the seed dressing process for growers. The seed dressing application rate is safe within the range of 10-60g / 100kg seeds, and the emergence rate, emergence speed and root development status after emergence are all significantly improved. It can also be used for other leguminous crops such as peanuts and beans. Description of the Drawings

[0026] Appendix Figure 1It is a photo of the soybean germination effect in the experiment of Example 1.

[0027] Appendix Figure 2 It is a photo of the soybean seedling emergence effect in the experiment of Example 1. Detailed implementation mode

[0028] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by this application.

[0029] For the instruments, reagents, materials, etc. involved in the following embodiments, unless otherwise specified, they are all conventional instruments, reagents, materials, etc. existing in the prior art and can be obtained through regular commercial channels. For the experimental methods, detection methods, etc. involved in the following embodiments, unless otherwise specified, they are all conventional experimental methods, detection methods, etc. existing in the prior art.

[0030] Example 1: Seed dressing adjuvant

[0031] The seed dressing adjuvant of this example is obtained by the following method:

[0032] (1) At room temperature, add 10 parts of boric acid to 160 parts of water, heat to 50 °C, stir continuously for 10 min, adjust the pH to 5.0 after complete dissolution, add 90 parts of fucoidan oligosaccharide, and continue to stir for 10 min until completely dissolved to obtain Solution A;

[0033] (2) At room temperature, add 15 parts of citric acid and 180 parts of ammonium molybdate to 360 parts of water, pressurize and heat to 75 °C, and keep stirring at this temperature for 30 min; adjust the pH to 4.5, add 67.5 parts of compound sugar alcohol, and continue to stir at this temperature for 30 min; add 10 parts of EDTA-Mo, continue to stir for 5 min and then stop heating to obtain Solution B;

[0034] (3) Add Solution A to Solution B, add 300 parts of water, adjust the pH to 4.3, heat to 40 °C, and stir continuously for 20 min;

[0035] (4) Stop heating, add 5 parts of surfactant while stirring, continue to stir for 10 min, and then stand still and fill to obtain this seed dressing adjuvant product. The surfactant is 32 parts of AEO-3 and 153 parts of AEO-15.

[0036] The pH regulator used in the above preparation is potassium hydroxide or sodium hydroxide.

[0037] Based on the above raw materials and methods, the following tests are carried out on the obtained seed dressing adjuvant, and the specific process and results are recorded as follows:

[0038] Test effect 1 - Soybean germination test

[0039] This test was conducted in the biological products laboratory of Shandong Shengbang Luye Chemical Co., Ltd. on November 10, 2024. Using soybean seeds as test materials, the seed dressing germination test method was adopted to observe the germination rate of soybean seeds and the number of germinated buds > 1 cm. A total of 6 treatments were set in the test. CK was the treatment with only the seed dressing agent, T-1 was the treatment with the seed dressing agent + a commercially available competing seed dressing fertilizer product, and T-2 - T-5 were the treatments with the seed dressing agent + different dosages of Example 1. The specific treatments and test results are shown in Table 1, and the dosages of the seed dressing agent in each treatment are the same. The test results show ( Figure 1 ), when the dosage of Example 1 is in the range of 10 - 40 g / 100 kg, the germination effect after soybean seed dressing is significantly better than that of CK and commercially available competitors, and there are slight differences in the effects among the dosages, but not significant. The germination rate of the T-2 - T-5 treatments increased significantly at 7 days, and the number of germinated buds > 1 cm showed an overall increasing trend.

[0040] Table 1 Soybean germination test of Example 1

[0041]

[0042] Example 2: Seed dressing adjuvant

[0043] The seed dressing adjuvant of this example is obtained by the following method:

[0044] (1) At room temperature, add 15 parts of boric acid to 160 parts of water, heat to 60 °C, stir continuously for 50 min, adjust the pH to 4.8 after complete dissolution, add 100 parts of fucoidan oligosaccharide, and continue to stir for 15 min until completely dissolved to obtain Solution A;

[0045] (2) At room temperature, add 20 parts of citric acid and 160 parts of ammonium molybdate to 320 parts of water, pressurize and heat to 75 °C, and keep stirring at this temperature for 20 min; adjust the pH to 4.2, add 55 parts of compound sugar alcohol, and continue to stir at this temperature for 20 min; add 15 parts of EDTA-Mo, continue to stir for 10 min and then stop heating to obtain Solution B;

[0046] (3) Add Solution A to Solution B, add 280 parts of water, adjust the pH to 4.5, heat to 50 °C, and stir continuously for 10 min;

[0047] (4) Stop heating, add 10 parts of surfactant while stirring, continue to stir for 15 min, and then stand still and fill to obtain this seed dressing adjuvant product. The surfactant is 4 parts of AEO-3 and 6 parts of AEO-15.

[0048] For the seed dressing adjuvant obtained based on the above raw materials and methods, the following tests were carried out, and the specific process and results are recorded as follows:

[0049] Test effect 2 - Soybean emergence test

[0050] This test was carried out in the greenhouse of Shandong Shengbang Luye Chemical Co., Ltd. on November 18, 2024. Using soybean seeds as test materials, the pot experiment method was adopted to observe the emergence rate and growth of soybean seeds. A total of 6 treatments were set up in the test. C1 was the treatment with the seed dressing agent alone, C2 was the seed dressing agent + the commercially available competing seed dressing fertilizer product, and C3 - C7 were the treatments with different dosages of the seed dressing agent + this patented adjuvant product. The specific treatments and test results are shown in Table 2. The test results show ( Figure 2 ), when the dosage of Example 1 is in the range of 10 - 40 g / 100 kg, the emergence effect of soybean seeds 14 days after seed dressing is significantly better than that of C1 and the commercially available competing products, and with the increase of dosage, the plant height of soybeans shows an increasing trend, which is significantly increased by 20.6 - 39.2% compared with the C1 control.

[0051] Table 2 Soybean germination test of Example 2

[0052]

Claims

1. A soybean seed dressing adjuvant, characterized in that The raw materials include brown algae oligosaccharide, chelated molybdenum composition, boric acid or borax, surfactant and water. The chelated molybdenum composition is prepared by chelating citric acid, complex sugar alcohol and ammonium molybdate and then mixing with EDTA-Mo.

2. A soybean seed dressing adjuvant according to claim 1, characterized in that: The brown algae oligosaccharide is produced by one-step enzymatic hydrolysis of sodium alginate as raw material using alginate lyase enzymatic hydrolysis technology, with a polymerization degree of 2-4, a molecular weight of 800-1000Da, and a brown algae oligosaccharide content of ≥95%.

3. A soybean seed dressing adjuvant according to claim 1, characterized in that: The brown algae oligosaccharide is composed of at least one of sorbitol, erythritol, xylitol and lactitol and mannitol in a ratio of 2 to 4:

1.

4. A soybean seed dressing adjuvant according to claim 1, characterized in that: In the chelating reaction, the ratio of citric acid, complex sugar alcohol and ammonium molybdate is 0.1-0.15:0.25-0.45:0.9-1.

2.

5. A soybean seed dressing adjuvant according to claim 1, characterized in that: The reaction pH value of the chelating reaction is in the range of 3.8 to 4.5, the reaction temperature is 65 to 75° C., and the reaction time is 40 to 60 minutes.

6. A soybean seed dressing adjuvant according to claim 1, characterized in that: The surfactant is a composition of AEO-3 and AEO-15, and the ratio thereof is 1:1.5-2.

7. A soybean seed dressing adjuvant according to claim 1, characterized in that: When the raw material is boric acid, the boric acid and water are first completely dissolved at 50-60° C. in a ratio of 1:8-10, and the pH is adjusted to 4.5-5.5, and then mixed with brown algae oligosaccharides and stirred evenly for use.

8. A soybean seed dressing adjuvant according to claim 1, characterized in that: Prepared by the following method: S1, add 10-20 parts of boric acid to 80-200 parts of water at room temperature, heat to 50-60°C, stir continuously for 5-10 minutes, adjust the pH to 4.5-5.5 after complete dissolution, add 90-100 parts of brown algae oligosaccharide, stir continuously for 10-15 minutes until complete dissolution, and use this as liquid A; S2, adding 15-22.5 parts of citric acid and 135-180 parts of ammonium molybdate to 260-360 parts of water at room temperature, heating under pressure to 65-75°C, maintaining this temperature and stirring for 20-30 minutes; adjusting the pH to 3.8-4.5, adding 37.5-67.5 parts of complex sugar alcohol, maintaining this temperature and stirring for 20-30 minutes; adding 10-20 parts of EDTA-Mo, continuing to stir for 5-10 minutes and then stopping heating, which is liquid B; S3, adding liquid A to liquid B, adding 200-300 parts of water, adjusting the pH to 3.8-4.8, heating to 40-50°C, and stirring continuously for 10-20 minutes; S4, stop heating, add 5 to 10 parts of surfactant while stirring, continue stirring for 10 to 15 minutes, let it stand and fill it to obtain the seed dressing auxiliary product.

9. A soybean seed dressing adjuvant according to claim 1, characterized in that: In specific application, the seed dressing adjuvant is mixed evenly with leguminous seeds at a seed dressing amount of 10-60g / 100kg and / or diluted 1500-3000 times for foliar spraying after emergence.

10. A method for preparing a soybean seed dressing adjuvant, characterized in that: The preparation method is as follows: liquid A is obtained by using boric acid or borax and brown algae oligosaccharide as raw materials; citric acid, complex sugar alcohol and ammonium molybdate are subjected to chelation reaction, and then mixed with EDTA-Mo to obtain a chelated molybdenum composition, which is used as liquid B; S3, liquid A, liquid B and water are mixed, and then a surfactant is added to obtain the product.

Images