Automatic detection device for cancer gene methylation markers

Abstract

The invention discloses an automatic detection device for cancer gene methylation markers, and relates to the technical field of cancer screening. The side end of the automatic nucleic acid extraction instrument is fixedly connected with a disinfection box; a deep hole plate is arranged in the disinfection box; the interior of the automatic nucleic acid extractor is fixedly connected with a first electric slide rail; a rotating rod is inserted into a hole in the deep hole plate through a first air cylinder, a clamping tooth belt is driven to rotate through a motor, a gear block is driven to rotate through rotation of the clamping tooth belt, the rotating rod and a cleaning brush are driven to rotate together through rotation of the gear block, the interior of the deep hole plate can be cleaned through rotation of the cleaning brush, and manual cleaning and disinfection are not needed; and after cleaning, a second electric sliding rail, a third electric sliding rail and a first air cylinder are matched to clean and disinfect the deep-hole plate row by row, the working process of cleaning and disinfecting the deep-hole plate is reduced, the labor cost and the time cost are reduced, and the cleaning efficiency is further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cancer screening, and particularly to an automated detection device for cancer gene methylation markers. Background Art

[0002] With the continuous increase in the global cancer incidence rate, early screening is of crucial significance for reducing cancer mortality and improving patient survival rate; cancer screening technologies have been continuously developed, from traditional imaging examinations and endoscopic examinations to modern molecular diagnostic technologies such as gene detection and liquid biopsy, providing more possibilities for early detection of cancer; liquid biopsy technology, due to its minimally invasive or even non-invasive advantages and good potential in detecting early cancer and pre-cancerous lesions, has brought new "problem-solving ideas" for cancer screening.

[0003] In modern cancer screening processes, the application of automated nucleic acid extractors has greatly improved the detection efficiency and accuracy; automated nucleic acid extractors can quickly and efficiently extract pure nucleic acids from various biological samples, providing high-quality templates for subsequent gene detection and molecular diagnosis; for example, the HONOR96NAE automated nucleic acid extractor of Huixiang Technology uses magnetic bead separation technology and can simultaneously purify 96 samples, which is widely used in institutions such as disease control centers, clinical disease diagnosis, third-party testing, scientific research, and universities.

[0004] However, the current automated nucleic acid extractors on the market have obvious deficiencies in the cleaning and disinfection of deep-well plates; deep-well plates need to be strictly cleaned and disinfected after each use to prevent cross-contamination between samples; but the existing cleaning and disinfection processes are often cumbersome and complex, requiring staff to wear strict protective clothing for operation, which not only increases the labor cost and time cost but also seriously affects the cleaning efficiency. Summary of the Invention

[0005] The purpose of the present invention is to provide an automated detection device for cancer gene methylation markers, so as to solve the technical problem that the existing cleaning and disinfection processes are often cumbersome and complex, requiring staff to wear strict protective clothing for operation, which not only increases the labor cost and time cost but also seriously affects the cleaning efficiency.

[0006] The technical problems to be solved by the present invention can be achieved through the following technical solutions:

[0007] An automated detection device for cancer gene methylation markers, comprising an automated nucleic acid extractor; a disinfection box is fixedly connected to the side end of the automated nucleic acid extractor; a deep well plate is arranged inside the disinfection box; a first electric slide rail is fixedly connected inside the automated nucleic acid extractor, and the deep well plate is slidably connected to the top end of the first electric slide rail; a drive box is arranged inside the disinfection box; a storage pool is formed inside the drive box; a drive cavity is formed below the storage pool inside the drive box; a motor is fixedly connected inside the drive box; a toothed belt is rotationally connected to the output end of the motor; a connecting column is arranged inside the drive box; the bottom end of the connecting column is rotationally connected to a rotating connecting block; a gear block is fixedly connected to the bottom end of the rotating connecting block; a rotating rod is arranged at the bottom end of the gear block; a cleaning brush is fixedly connected to the outer side wall of the rotating rod, and the cleaning brush is arranged above the deep well plate.

[0008] As a further scheme of the present invention: a second electric slide rail is fixedly connected to the inner side wall of the disinfection box; a third electric slide rail is slidably connected to the side end of the second electric slide rail; a first air cylinder is slidably connected to the bottom end of the third electric slide rail; the bottom end of the first air cylinder is fixedly connected to the drive box.

[0009] As a further scheme of the present invention: a disinfectant solution box is fixedly connected to the inner side wall of the disinfection box; a telescopic hose is fixedly connected to the top end of the disinfectant solution box; the other end of the telescopic hose is connected to the drive box.

[0010] As a further scheme of the present invention: a second air cylinder is fixedly connected to the inside of the disinfection box; a positioning plate is fixedly connected to the side end of the second air cylinder, and the positioning plate is arranged on one side of the deep well plate.

[0011] As a further scheme of the present invention: a drying shell is fixedly connected to the side end of the drive box; a plurality of sponge rods are installed at the bottom end of the drying shell.

[0012] As a further scheme of the present invention: an ultraviolet lamp is slidably connected to the bottom end of the third electric slide rail, and the ultraviolet lamp is arranged on one side of the drive box.

[0013] As a further scheme of the present invention: a valve is fixedly connected to the inside of the drive box, and the valve is arranged below the storage pool; an inner groove is formed inside the connecting column; a through groove is formed inside the rotating rod, and the through groove is communicated with the inner groove; a discharge groove is formed inside the rotating rod, and the discharge groove is communicated with the through groove.

[0014] As a further scheme of the present invention: the top end of the rotating rod is threadedly connected to the gear block, and the cleaning brush is arranged below the gear block.

[0015] Advantages of the present invention: Through the cooperation of the second electric slide rail, the third electric slide rail and the first cylinder, the drive box is moved above the deep well plate. The first cylinder inserts the rotating rod into the holes inside the deep well plate. The liquid inside the storage tank is conveyed to the rotating rod through the valve, and the liquid flows from the rotating rod into the deep well plate. The motor rotates the toothed belt, and the rotation of the toothed belt causes the gear block to rotate. The rotation of the gear block further causes the rotating rod and the cleaning brush to rotate together. The rotation of the cleaning brush can clean the inside of the deep well plate. Combined with the liquid discharged from the storage tank, it can improve the cleaning and disinfection operation of the deep well plate, eliminating the need for manual cleaning and disinfection. After cleaning, the cooperation of the second electric slide rail, the third electric slide rail and the first cylinder is used to clean and disinfect the deep well plate row by row, reducing the workflow of cleaning and disinfecting the deep well plate, reducing labor costs and time costs, and also improving the cleaning efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention will be further described below with reference to the drawings.

[0017] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 is a schematic diagram of the disinfection box structure of the present invention;

[0019] Figure 3 is a schematic diagram of the drying shell structure of the present invention;

[0020] Figure 4 is a schematic diagram of the drive box structure of the present invention;

[0021] Figure 5 is the present invention Figure 4 magnified view of part A;

[0022] In the figure: 1, automated nucleic acid extractor; 2, disinfection box; 3, deep well plate; 4, first electric slide rail; 5, second electric slide rail; 6, third electric slide rail; 7, first cylinder; 8, drive box; 9, disinfectant solution tank; 10, telescopic hose; 11, storage tank; 12, drive cavity; 13, motor; 14, toothed belt; 15, rotating rod; 16, cleaning brush; 17, connecting column; 18, rotating connection block; 19, gear block; 20, inner groove; 201, through groove; 21, discharge groove; 22, valve; 23, drying shell; 24, sponge rod; 25, second cylinder; 26, positioning plate; 27, ultraviolet lamp. DETAILED DESCRIPTION OF THE INVENTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0024] As Figures 1 - 5 shown, an automated detection device for cancer gene methylation markers includes an automated nucleic acid extractor 1; a disinfection box 2 is fixedly connected to the side end of the automated nucleic acid extractor 1; a deep well plate 3 is arranged inside the disinfection box 2; a first electric slide rail 4 is fixedly connected inside the automated nucleic acid extractor 1, and the deep well plate 3 is slidably connected to the top of the first electric slide rail 4; a drive box 8 is arranged inside the disinfection box 2; a storage pool 11 is formed inside the drive box 8; a drive cavity 12 is formed below the storage pool 11 inside the drive box 8; a motor 13 is fixedly connected inside the drive box 8; the output end of the motor 13 is rotationally connected to a toothed belt 14; there is a connecting column 17 inside the drive box 8; the bottom end of the connecting column 17 is rotationally connected to a rotary connecting block 18; the bottom end of the rotary connecting block 18 is fixedly connected to a gear block 19; a rotary rod 15 is arranged at the bottom end of the gear block 19; a cleaning brush 16 is fixedly connected to the outer side wall of the rotary rod 15, and the cleaning brush 16 is arranged above the deep well plate 3.

[0025] Through the cooperation of the second electric slide rail 5, the third electric slide rail 6 and the first cylinder 7, the drive box 8 is moved above the deep well plate 3. Through the first cylinder 7, the rotary rod 15 is inserted into the holes inside the deep well plate 3. The liquid inside the storage pool 11 is delivered to the rotary rod 15 through a valve 22. The liquid flows from the rotary rod 15 into the inside of the deep well plate 3. The motor 13 is used to rotate the toothed belt 14. The rotation of the toothed belt 14 causes the gear block 19 to rotate. The rotation of the gear block 19 further causes the rotary rod 15 and the cleaning brush 16 to rotate together. The rotation of the cleaning brush 16 can clean the inside of the deep well plate 3. The cooperation with the liquid discharged from the storage pool 11 can improve the cleaning and disinfection operation of the deep well plate 3. There is no need for manual cleaning and disinfection. After cleaning, the deep well plate 3 is cleaned and disinfected row by row through the cooperation of the second electric slide rail 5, the third electric slide rail 6 and the first cylinder 7, reducing the work process of cleaning and disinfecting the deep well plate 3, reducing the labor cost and time cost, and also improving the cleaning efficiency.

[0026] A second electric slide rail 5 is fixedly connected to the inner side wall of the disinfection box 2; a third electric slide rail 6 is slidably connected to the side end of the second electric slide rail 5; a first cylinder 7 is slidably connected to the bottom end of the third electric slide rail 6; the bottom end of the first cylinder 7 is fixedly connected to the drive box 8.

[0027] The position of the third electric slide rail 6 can be adjusted and moved by the second electric slide rail 5. The position of the first air cylinder 7 can be slidably adjusted by the third electric slide rail 6. The position height of the drive box 8 can be adjusted by the first air cylinder 7. The cooperation of the second electric slide rail 5, the third electric slide rail 6 and the first air cylinder 7 can move the drive box 8 above the deep hole plate 3 for cleaning and disinfection work without manual operation;

[0028] The inner side wall of the disinfection box 2 is fixedly connected with a disinfectant solution tank 9; the top end of the disinfectant solution tank 9 is fixedly connected with a telescopic hose 10; the other end of the telescopic hose 10 is connected with the drive box 8.

[0029] The disinfectant solution tank 9 stores 70% ethanol liquid inside. A water pump is provided inside the disinfectant solution tank 9. The water pump transports the liquid to the telescopic hose 10. The liquid enters the inside of the drive box 8 through the telescopic hose 10. The telescopic hose 10 can be telescoped and will not affect the position movement of the drive box 8. The disinfectant solution tank 9 can provide disinfectant water for the drive box 8;

[0030] The second air cylinder 25 is fixedly connected to the inside of the disinfection box 2; a positioning plate 26 is fixedly connected to the side end of the second air cylinder 25, and the positioning plate 26 is arranged on one side of the deep hole plate 3.

[0031] When the deep hole plate 3 is moved into the disinfection box 2 by the first electric slide rail 4, at this time, the positioning plate 26 is moved towards the deep hole plate 3 by the second air cylinder 25, and the deep hole plate 3 is clamped and fixed by the positioning plate 26, which is convenient for the cleaning and disinfection operation of the deep hole plate 3 and avoids the position change of the deep hole plate 3 during the disinfection process, affecting the cleaning and disinfection operation;

[0032] The side end of the drive box 8 is fixedly connected with a drying shell 23; a plurality of sponge rods 24 are installed at the bottom end of the drying shell 23.

[0033] The disinfectant solution is ejected into the inside of the deep hole plate 3 through the discharge groove 21. After the cleaning brush 16 cleans the deep hole plate 3, there will be some residual liquid inside the deep hole plate 3. When the rotating rod 15 cleans the next row of the deep hole plate 3, the drying shell 23 will move along with the drive box 8, and the sponge rod 24 will be inserted into the inside of the deep hole plate 3 to absorb the residual liquid. A motor 13 and a toothed belt 14 are also provided inside the drying shell 23. The sponge rod 24 can rotate, and the operation mode of the sponge rod 24 is the same as that of the rotating rod 15, and it rotates and operates through the motor 13 and the toothed belt 14;

[0034] The bottom end of the third electric slide rail 6 is slidably connected with an ultraviolet lamp 27, and the ultraviolet lamp 27 is arranged on one side of the drive box 8.

[0035] After the cleaning brush 16 disinfects the deep well plate 3, the position of the drive box 8 is moved away from the deep well plate 3 through the second electric slide rail 5, the third electric slide rail 6 and the first cylinder 7, and the ultraviolet lamp 27 is moved above the deep well plate 3. The deep well plate 3 is disinfected by the ultraviolet lamp 27, which can improve the disinfection effect on the deep well plate 3. After the deep well plate 3 is disinfected, the deep well plate 3 is moved into the automatic nucleic acid extractor 1 through the first electric slide rail 4;

[0036] A valve 22 is fixedly connected inside the drive box 8, and the valve 22 is arranged below the storage tank 11; an inner groove 20 is opened inside the connecting column 17; a through groove 201 is opened inside the rotating rod 15, and the through groove 201 communicates with the inner groove 20; a discharge groove 21 is opened inside the rotating rod 15, and the discharge groove 21 communicates with the through groove 201.

[0037] The valve 22 is used to control the disinfectant liquid inside the storage tank 11 to enter the inner groove 20. The disinfectant liquid enters the inside of the through groove 201 through the inner groove 20, and the through groove 201 discharges the disinfectant liquid into the deep well plate 3 through the discharge groove 21. The rotation of the rotating rod 15 can more easily throw the disinfectant liquid into the deep well plate 3, and cooperate with the rotation of the cleaning brush 16 for cleaning operations;

[0038] The top end of the rotating rod 15 is threadedly connected to the gear block 19, and the cleaning brush 16 is arranged below the gear block 19.

[0039] The rotating rod 15 and the gear block 19 are threadedly connected. The cleaning brush 16 on the rotating rod 15 can clean the deep well plate 3. Regular maintenance or replacement is required to ensure the cleaning effect of the drive box 8 on the deep well plate 3. Rotating the rotating rod 15 can disassemble it from the gear block 19.

[0040] Working principle of the present invention: When the deep well plate 3 needs to be cleaned after working inside the automated nucleic acid extractor 1, the deep well plate 3 is moved into the disinfection box 2 by the first electric slide rail 4. The automated nucleic acid extractor 1 communicates with the disinfection box 2. One side of the disinfection box 2 is provided with a sealing door, which can be closed during operation. The position of the third electric slide rail 6 can be adjusted and moved by the second electric slide rail 5. The position of the first cylinder 7 can be slidably adjusted by the third electric slide rail 6. The position height of the drive box 8 can be adjusted by the first cylinder 7. The cooperation of the second electric slide rail 5, the third electric slide rail 6 and the first cylinder 7 can move the drive box 8 above the deep well plate 3 for cleaning and disinfection work without manual operation. The internal of the disinfectant liquid tank 9 stores 70% ethanol liquid. A water pump is provided inside the disinfectant liquid tank 9. The water pump conveys the liquid to the telescopic hose 10. The liquid enters the internal of the drive box 8 through the telescopic hose 10. The telescopic hose 10 can be telescopic and will not affect the position movement of the drive box 8. The disinfectant liquid tank 9 can provide disinfectant water for the drive box 8. When the deep well plate 3 is moved into the disinfection box 2 by the first electric slide rail 4, at this time, the positioning plate 26 is moved towards the deep well plate 3 by the second cylinder 25, and the deep well plate 3 is clamped and fixed by the positioning plate 26, which is convenient for the cleaning and disinfection operation of the deep well plate 3 and avoids the position change of the deep well plate 3 during the disinfection process, affecting the cleaning and disinfection operation. The drive box 8 is moved above the deep well plate 3 through the cooperation of the second electric slide rail 5, the third electric slide rail 6 and the first cylinder 7. The rotating rod 15 is inserted into the holes inside the deep well plate 3 by the first cylinder 7. The liquid inside the storage pool 11 is conveyed to the rotating rod 15 through the valve 22. The liquid flows from the rotating rod 15 into the deep well plate 3. The motor 13 makes the toothed belt 14 rotate. The rotation of the toothed belt 14 makes the gear block 19 rotate. The rotation of the gear block 19 makes the rotating rod 15 and the cleaning brush 16 rotate together. The rotation of the cleaning brush 16 can clean the inside of the deep well plate 3. The cooperation with the liquid discharged from the storage pool 11 can improve the cleaning and disinfection operation of the deep well plate 3 without manual cleaning and disinfection. After cleaning, the deep well plate 3 is cleaned and disinfected row by row through the cooperation of the second electric slide rail 5, the third electric slide rail 6 and the first cylinder 7, reducing the working process of cleaning and disinfecting the deep well plate 3, reducing the labor cost and time cost, and also improving the cleaning efficiency. The disinfectant liquid is ejected into the deep well plate 3 through the discharge groove 21. After the cleaning brush 16 cleans the deep well plate 3, there will be some residual liquid inside the deep well plate 3. When the rotating rod 15 cleans the next row of the deep well plate 3, the drying shell 23 will move along with the drive box 8, and the sponge rod 24 will be inserted into the deep well plate 3 to absorb the residual liquid. There is also a motor 13 and a toothed belt 14 inside the drying shell 23. The sponge rod 24 can rotate. The operation mode of the sponge rod 24 is the same as that of the rotating rod 15, and it rotates through the motor 13 and the toothed belt 14.After the cleaning brush 16 disinfects the deep-well plate 3, the position of the drive box 8 is moved away from the deep-well plate 3 through the second electric slide rail 5, the third electric slide rail 6 and the first cylinder 7, and the ultraviolet lamp 27 is moved above the deep-well plate 3. The deep-well plate 3 is disinfected by the ultraviolet lamp 27, which can improve the disinfection effect on the deep-well plate 3. After the deep-well plate 3 is disinfected, the deep-well plate 3 is moved into the automatic nucleic acid extractor 1 through the first electric slide rail 4.;

[0041] The above has described an embodiment of the present invention in detail, but the content described is only the preferred embodiment of the present invention and cannot be considered as limiting the scope of implementation of the present invention. All equivalent changes and improvements made according to the scope of the application of the present invention should still fall within the scope covered by the patent of the present invention.

Claims

1. An automated detection device for cancer gene methylation markers, characterized in that: The invention comprises an automated nucleic acid extractor (1); a disinfection box (2) is fixedly connected to the side end of the automated nucleic acid extractor (1); a deep-well plate (3) is provided inside the disinfection box (2); a No. 1 electric slide rail (4) is fixedly connected inside the automated nucleic acid extractor (1), and the deep-well plate (3) is slidably connected to the top of the No. 1 electric slide rail (4); a drive box (8) is provided inside the disinfection box (2); a storage pool (11) is provided inside the drive box (8); a drive cavity (12) is provided below the storage pool (11) inside the drive box (8); ); a motor (13) is fixedly connected to the interior of the driving box (8); the output end of the motor (13) is rotatably connected to a toothed belt (14); a connecting column (17) is provided inside the driving box (8); the bottom end of the connecting column (17) is rotatably connected to a rotating connecting block (18); the bottom end of the rotating connecting block (18) is fixedly connected to a gear block (19); a rotating rod (15) is provided at the bottom end of the gear block (19); a cleaning brush (16) is fixedly connected to the outer wall of the rotating rod (15), and the cleaning brush (16) is provided above the deep hole plate (3).

2. The automated detection device for cancer gene methylation markers according to claim 1, characterized in that: The inner wall of the disinfection box (2) is fixedly connected to a No. 2 electric slide rail (5); the side end of the No. 2 electric slide rail (5) is slidably connected to a No. 3 electric slide rail (6); the bottom end of the No. 3 electric slide rail (6) is slidably connected to a No. 1 cylinder (7); the bottom end of the No. 1 cylinder (7) is fixedly connected to a drive box (8).

3. The automated detection device for cancer gene methylation markers according to claim 1, characterized in that: A disinfectant tank (9) is fixedly connected to the inner wall of the disinfectant tank (2); a telescopic hose (10) is fixedly connected to the top of the disinfectant tank (9); and the other end of the telescopic hose (10) is connected to the drive box (8).

4. The automated detection device for cancer gene methylation markers according to claim 1, characterized in that: A No. 2 air cylinder (25) is fixedly connected inside the disinfection box (2); a positioning plate (26) is fixedly connected to the side end of the No. 2 air cylinder (25), and the positioning plate (26) is arranged on one side of the deep hole plate (3).

5. The automated detection device for cancer gene methylation markers according to claim 1, characterized in that: A drying shell (23) is fixedly connected to the side end of the driving box (8); and a plurality of groups of sponge rods (24) are installed at the bottom end of the drying shell (23).

6. The automated detection device for cancer gene methylation markers according to claim 2, characterized in that: The bottom end of the third electric slide rail (6) is slidably connected to an ultraviolet lamp (27), which is arranged on one side of the drive box (8).

7. The automated detection device for cancer gene methylation markers according to claim 1, characterized in that: A valve (22) is fixedly connected to the interior of the driving box (8), and the valve (22) is arranged below the storage tank (11); an inner groove (20) is provided inside the connecting column (17); a through groove (201) is provided inside the rotating rod (15), and the through groove (201) is communicated with the inner groove (20); a discharge groove (21) is provided inside the rotating rod (15), and the discharge groove (21) is communicated with the through groove (201).

8. The automated detection device for cancer gene methylation markers according to claim 1, characterized in that: The top end of the rotating rod (15) is threadedly connected to the gear block (19), and the cleaning brush (16) is arranged below the gear block (19).

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