Stem cell culture dish

Abstract

The invention discloses a stem cell culture dish, and relates to the technical field of cell culture dishes, the stem cell culture dish comprises a base, a heating mechanism is arranged on the upper surface of the base, the heating mechanism comprises a water tank, the water tank is fixedly connected to the upper surface of the base, and a breather pipe penetrates through the outer side surface of the water tank. The end, away from the water tank, of the ventilation pipe is fixedly connected with a transfer box, and by arranging the heating mechanism, the temperature in the culture dish can be heated during stem cell culture, so that the culture dish is kept at a proper temperature, and the culture of stem cells can be promoted; hot air generated in the inner cavity of the water tank can be guided; a temperature adjusting mechanism is arranged on the side, away from the ventilation pipe, of the transfer box, the temperature adjusting mechanism comprises an arc-shaped pipe, the arc-shaped pipe penetrates through the side, away from the ventilation pipe, of the transfer box, a placement frame is fixedly connected to the lower surface of the arc-shaped pipe, and the effect of heating different positions of the culture dish is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture dishes, and particularly to a stem cell culture dish. Background Art

[0002] As a key instrument for cell culture experiments, cell culture dishes play a crucial role in the field of life science research. Most of them are made of polystyrene plastic, which has good transparency, enabling experimenters to directly observe the growth state of cells through the dish wall, such as cell morphology, distribution, and proliferation. The surface of the culture dish is usually treated specially, like TC treatment (tissue culture treatment), which can make cells adhere and grow better, creating a stable "habitat" for cells. In terms of specifications, there are different diameters available to meet diverse experimental needs. Small ones with a diameter of 35mm are suitable for small-scale culture or precious cell samples, while large ones up to 150mm are used for batch culture of a large number of cells. When in use, the principle of aseptic operation must be strictly followed. The culture dish should be pre-sterilized by high-pressure steam to ensure no contamination by miscellaneous bacteria, providing a pure environment for cell growth.

[0003] In cell culture experiments, commercially available cell culture dishes cannot independently control the temperature of a single culture dish. From the perspective of cell growth characteristics, different types of cells have strict requirements for temperature. For example, the suitable growth temperature of mammalian cells is usually around 37°C. Deviating from this temperature range will disrupt the physiological activities of cells. Without an independent temperature control function, when the laboratory environment temperature fluctuates, the growth rate of cells in the culture dish will slow down, the enzyme activity will be inhibited, and the material metabolism will be imbalanced. In a high-temperature environment, the risk of protein denaturation in cells increases, which may lead to cell death; at low temperatures, the fluidity of the cell membrane becomes poor, the transport of nutrients is blocked, and cell proliferation stagnates. Summary of the Invention

[0004] To achieve the above object, the present invention is realized through the following technical solutions: A stem cell culture dish, comprising a base, on the upper surface of the base is provided a heating mechanism, the heating mechanism includes a water tank, the water tank is fixedly connected to the upper surface of the base, a ventilation pipe penetrates through the outer side surface of the water tank, and one end of the ventilation pipe away from the water tank is fixedly connected to a transfer tank. By setting the heating mechanism, during the cultivation of stem cells, the temperature inside the culture dish can be heated, so that the culture dish maintains an appropriate temperature, which can promote the cultivation of stem cells. By setting the ventilation pipe and the transfer tank, the hot air generated in the inner cavity of the water tank can be guided; on one side of the transfer tank away from the ventilation pipe is provided a temperature adjustment mechanism, the temperature adjustment mechanism includes an arc-shaped pipe, the arc-shaped pipe penetrates through one side of the transfer tank away from the ventilation pipe, a placement rack is fixedly connected to the lower surface of the arc-shaped pipe, a plurality of air holes are opened on the upper surface of the arc-shaped pipe, a semi-circular pipe is slidably connected in the inner cavity of the arc-shaped pipe, an exhaust port penetrates through the upper surface of the semi-circular pipe, and the number of the exhaust ports is the same as the number of the air holes opened on the upper surface of the arc-shaped pipe. By setting the temperature adjustment mechanism, under control, the heat generated by the heating mechanism can flow to the bottom, outer side and top of the culture dish respectively, so that under control, the effect of heating different positions of the culture dish can be achieved. By setting the arc-shaped pipe, it can be connected to the transfer tank, so that the hot air generated by heating the water tank can enter the inner cavity of the arc-shaped pipe and wrap the culture dish. By setting the semi-circular pipe, it can rotate in the inner cavity of the arc-shaped pipe, and then adjust the position of the exhaust port to achieve the effect of adjusting the direction of the hot air flow; on the upper surface of the placement rack is provided a culture dish mechanism, the culture dish mechanism includes a culture dish bottom shell and a culture dish cover, the culture dish bottom shell is located on the upper surface of the placement rack, and the culture dish cover is movably connected to the opening of the culture dish bottom shell. By setting the culture dish mechanism, the stem cells to be cultivated can be placed, and under the action of the temperature adjustment mechanism and the heating mechanism, the stem cells can be at an appropriate temperature. By setting the culture dish bottom shell and the culture dish cover, the stem cells to be cultivated can be placed.

[0005] Preferably, a one-way valve penetrates through the top of the outer side surface of the water tank, one end of the one-way valve is fixedly connected to a water inlet pipe, the heating mechanism further includes a high-voltage DC power supply, the high-voltage DC power supply is fixedly connected to the inner wall of the base, and the output end of the high-voltage DC power supply is connected to a heating plate through a wire. The heating plate is closely attached to the lower surface of the water tank. By setting the one-way valve, the water flow can only enter the inner cavity of the water tank through the water inlet pipe, and the water flow or air in the inner cavity of the water tank will not be discharged. By setting the high-voltage DC power supply, during operation, the current can flow to the heating plate, so that the resistance wire inside the heating plate generates heat, and then heats the water tank.

[0006] Preferably, a cushion strip is fixedly connected to the upper surface of the placement rack. The cushion strip is in extrusion fit with the lower surface of the bottom shell of the culture dish. A connecting plate is fixedly connected to the outer surface of the placement rack. A limiting frame is fixedly connected to the end of the connecting plate. A rotating rod is rotatably connected to the inner cavity of the limiting frame. An anti-slip wheel is fixedly connected to the outer surface of the rotating rod. The anti-slip wheel is made of rubber and has many anti-slip grooves on its outer surface. By providing the cushion strip, the bottom of the bottom shell of the culture dish can be protected to prevent the bottom of the glass bottom shell of the culture dish from being damaged by knocking. By providing the limiting frame, the rotating rod can be limited so that the rotating rod can rotate stably in the inner cavity of the limiting frame. By providing the anti-slip wheel, the bottom shell of the culture dish can be supported, so that when the bottom shell of the culture dish rotates, it can rotate more stably.

[0007] Preferably, a track groove is provided on the outer side surface of the arc-shaped tube. A handle is fixedly connected to the outer side surface of the semi-circular tube. The handle is slidably connected to the track groove provided on the outer side surface of the arc-shaped tube. The exhaust port is movably connected to the ventilation hole provided on the upper surface of the arc-shaped tube. A sealing ring is fixedly connected to the upper surface of the exhaust port. By providing the track groove, the handle can be limited so that the handle can move stably in the track groove provided on the outer side surface of the arc-shaped tube. By providing the handle, the movement of the semi-circular tube can be facilitated. By providing the sealing ring, the sealing performance of the top of the exhaust port can be increased.

[0008] Preferably, connection boxes are fixedly connected to the ventilation holes provided on the upper surface of the arc-shaped tube. An exhaust hood penetrates through the outer side surface of the connection box. The exhaust hood is made of rubber. The opening of the exhaust hood is in frictional fit with the outer surface of the bottom shell of the culture dish. A first fixing plate is fixedly connected to the inner wall of the connection box. A sealing plate is fixedly connected to the outer surface of the first fixing plate. The sealing plate is in extrusion fit with the sealing ring. By providing the connection box, the ventilation holes provided on the upper surface of the arc-shaped tube can be wrapped. By providing the exhaust hood, when the exhaust port is not in contact with the sealing plate, the hot air discharged from the arc-shaped tube can be discharged to the outer side surface of the bottom shell of the culture dish through the exhaust hood, so that the temperature of the outer wall of the bottom shell of the culture dish can be increased. By providing the sealing plate, the top of the exhaust port can be sealed, so that the hot air will not be discharged from the exhaust port and will always be in the inner cavity of the arc-shaped tube, thereby increasing the temperature of the arc-shaped tube and then heating the periphery of the bottom shell of the culture dish.

[0009] Preferably, a second fixed plate is fixedly connected to the side of the connection box away from the first fixed plate. An air-permeable ring is fixedly connected to the outer surface of the second fixed plate. The air-permeable ring is in pressing fit with the upper surface of the sealing ring. A straight pipe is fixedly connected to the upper surface of the air-permeable ring. The straight pipe penetrates the connection box. One end of the straight pipe penetrating the connection box is fixedly connected to a connecting pipe. By providing the air-permeable ring, when the semi-circular pipe rotates and the exhaust port contacts the lower surface of the air-permeable ring, the hot air in the inner cavity of the arc-shaped pipe can enter the inner cavity of the straight pipe through the air-permeable ring and finally be discharged from the connecting pipe.

[0010] Preferably, a first telescopic pipe is fixedly connected to the upper surface of the culture dish cover. The first telescopic pipe is made of rubber and has a certain deformation ability. A top box is fixedly connected to the upper surface of the first telescopic pipe. One end of the connecting pipe away from the straight pipe penetrates the top box. A hexagonal frame is fixedly connected to the center of the lower surface of the culture dish bottom shell. By providing the first telescopic pipe, a certain deformation can be generated, so that the top box can drive the culture dish cover to move up and down, which is convenient for closing the opening of the culture dish bottom shell by moving the culture dish cover up and down after the culture dish bottom shell is placed on the upper surface of the placement rack.

[0011] Preferably, a rotating mechanism is arranged on the upper surface of the water tank. The rotating mechanism includes a second telescopic pipe. The second telescopic pipe penetrates the center of the upper surface of the water tank. The second telescopic pipe is made of rubber and has a certain deformation ability. A support rod is fixedly connected to the top end of the second telescopic pipe. A limiting ring is fixedly connected to the end of the support rod. By providing the rotating mechanism, when the culture dish bottom shell needs to be rotated, the steam generated by the water tank can be used to control the stable rotation of the culture dish bottom shell, so that the temperature in the inner cavity of the culture dish bottom shell can rise stably. By providing the second telescopic pipe, the effect of moving up and down can be generated under extrusion and stretching.

[0012] Preferably, a sliding frame is fixedly connected to the outer surface of the limiting ring. The number of the sliding frames is three, and the three sliding frames are evenly distributed. The ends of the three sliding frames are all slidably connected to a limiting rod. The top end of the limiting rod is fixedly connected to the lower surface of the placement rack. By providing the limiting rod, the sliding frame can be limited, so that the sliding frame can move vertically up and down, thereby adjusting the position of the limiting ring.

[0013] Preferably, a circular plate is fixedly connected to the bottom of the inner wall of the second telescopic tube. The side of the upper surface of the circular plate penetrates through the pressurizing port. A rotating frame is rotatably connected to the inner cavity of the limiting ring. A hexagonal prism is fixedly connected to the upper surface of the rotating frame. The hexagonal prism is frictionally and adaptively engaged with the inner wall of the hexagonal frame. A rotating column is fixedly connected to the lower surface of the rotating frame. An inclined plate is fixedly connected to the outer surface of the rotating column. The inclined plate is located directly above the pressurizing port. The number of the inclined plates is several, and several of the inclined plates are evenly distributed. By providing the pressurizing port, when the water in the inner cavity of the water tank is heated to generate water vapor, the water vapor can quickly discharge from the pressurizing port, so that the water vapor presses on the inclined plate. When the inclined plate is impacted by the airflow, the rotating column will drive the rotating frame to rotate, so that the hexagonal prism rotates, and then drives the hexagonal frame to rotate, and finally makes the bottom shell of the culture dish rotate stably.

[0014] The present invention provides a stem cell culture dish. It has the following beneficial effects:

[0015] First, in this stem cell culture dish, by providing a heating mechanism, the temperature inside the culture dish can be heated during the culture of stem cells, so that the culture dish maintains an appropriate temperature, which can promote the culture of stem cells. By providing an air pipe and a transfer box, the hot air generated in the inner cavity of the water tank can be guided.

[0016] Second, in this stem cell culture dish, by providing a temperature regulating mechanism, under control, the heat generated by the heating mechanism can be respectively directed to the bottom, outside and top of the culture dish, so that the effect of heating different positions of the culture dish can be achieved under control. By providing an arc-shaped pipe, it can be connected to the transfer box, so that the hot air generated by heating the water tank can enter the inner cavity of the arc-shaped pipe and wrap the culture dish. By providing a semi-circular pipe, it can rotate in the inner cavity of the arc-shaped pipe, and then adjust the position of the exhaust port to achieve the effect of adjusting the flow direction of the hot steam.

[0017] Third, in this stem cell culture dish, by providing a culture dish mechanism, the stem cells to be cultured can be placed, and under the action of the temperature regulating mechanism and the heating mechanism, the stem cells can be at an appropriate temperature. By providing a bottom shell of the culture dish and a culture dish cover, the stem cells to be cultured can be placed.

[0018] IV. For this stem cell culture dish, by providing a connecting box, the ventilation holes opened on the upper surface of the arc-shaped tube can be wrapped. By providing an exhaust hood, when the exhaust port is not in contact with the sealing plate, the hot air discharged from the arc-shaped tube can be discharged to the outer side of the culture dish bottom shell through the exhaust hood, so that the temperature of the outer wall of the culture dish bottom shell can be increased. By providing a sealing plate, the top of the exhaust port can be sealed, so that the hot air will not be discharged from the exhaust port and will always be in the inner cavity of the arc-shaped tube, thereby increasing the temperature of the arc-shaped tube and then heating the periphery of the culture dish bottom shell.

[0019] V. For this stem cell culture dish, by providing a rotating mechanism, when it is necessary to rotate the culture dish bottom shell, the steam generated by the water tank can be used to control the stable rotation of the culture dish bottom shell, so that the temperature in the inner cavity of the culture dish bottom shell can rise stably. By providing a second telescopic tube, it can produce an up-and-down movement effect under extrusion and stretching. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic diagram of the external structure of a stem cell culture dish of the present invention;

[0021] Figure 2 is a side view of the structure of a stem cell culture dish of the present invention;

[0022] Figure 3 is a schematic diagram of the structure of the heating mechanism of the present invention;

[0023] Figure 4 is a schematic diagram of a partial structure of a stem cell culture dish of the present invention;

[0024] Figure 5 is of the present invention Figure 4 a schematic enlarged view of Structure A;

[0025] Figure 6 is a schematic diagram of the structure of the temperature adjustment mechanism of the present invention;

[0026] Figure 7 is a schematic diagram of a partial structure of the temperature adjustment mechanism of the present invention;

[0027] Figure 8 is of the present invention Figure 6 a schematic enlarged view of Structure B;

[0028] Figure 9 is a schematic diagram of the structure of the culture dish mechanism of the present invention;

[0029] Figure 10 is a schematic diagram of the structure of the rotating mechanism of the present invention;

[0030] Figure 11 is a schematic diagram of a partial cross-sectional structure of the rotating mechanism of the present invention.

[0031] In the figure: 1, base; 2, heating mechanism; 3, placement rack; 4, temperature adjustment mechanism; 5, cushion strip; 6, petri dish mechanism; 7, rotation mechanism; 8, connecting plate; 9, limiting frame; 10, rotating rod; 11, anti-slip wheel; 21, water tank; 22, one-way valve; 23, water inlet pipe; 24, high-voltage DC power supply; 25, heating plate; 26, ventilation pipe; 27, transfer box; 41, arc-shaped pipe; 42, track groove; 43, semi-circular pipe; 44, grip; 45, exhaust port; 46, sealing ring; 47, connection box; 48, exhaust hood; 49, first fixing plate; 410, sealing plate; 411, second fixing plate; 412, ventilation ring; 413, straight pipe; 414, connecting pipe; 61, petri dish bottom shell; 62, hexagonal frame; 63, petri dish cover; 64, first telescopic pipe; 65, top box; 71, second telescopic pipe; 72, circular plate; 73, pressurizing port; 74, limiting rod; 75, sliding rack; 76, limiting ring; 77, rotating frame; 78, support rod; 79, rotating column; 710, inclined plate; 711, hexagonal prism. Specific implementation manner

[0032] The present invention will be further described in detail below with reference to the accompanying drawings and specific implementation manners. The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limit the present invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present invention, and enable those of ordinary skill in the art to understand the present invention and thus design various embodiments with various modifications suitable for specific purposes.

[0033] The first embodiment is as Figures 1 - 5As shown in the figure, the present invention provides a technical solution: a stem cell culture dish, including a base 1. On the upper surface of the base 1, there is a heating mechanism 2. The heating mechanism 2 includes a water tank 21. The water tank 21 is fixedly connected to the upper surface of the base 1. A ventilation pipe 26 penetrates through the outer side of the water tank 21. One end of the ventilation pipe 26 away from the water tank 21 is fixedly connected to a transfer box 27. By setting the heating mechanism 2, during the cultivation of stem cells, the temperature inside the culture dish can be heated, so that the culture dish maintains an appropriate temperature, which can promote the cultivation of stem cells. By setting the ventilation pipe 26 and the transfer box 27, the hot steam generated in the inner cavity of the water tank 21 can be guided. On one side of the transfer box 27 away from the ventilation pipe 26, there is a temperature adjustment mechanism 4. The temperature adjustment mechanism 4 includes an arc-shaped pipe 41. The arc-shaped pipe 41 penetrates through one side of the transfer box 27 away from the ventilation pipe 26. A placement rack 3 is fixedly connected to the lower surface of the arc-shaped pipe 41. A plurality of ventilation holes are opened on the upper surface of the arc-shaped pipe 41. A semi-circular pipe 43 is slidably connected to the inner cavity of the arc-shaped pipe 41. An exhaust port 45 penetrates through the upper surface of the semi-circular pipe 43. The number of the exhaust ports 45 is the same as the number of the ventilation holes opened on the upper surface of the arc-shaped pipe 41. By setting the temperature adjustment mechanism 4, under control, the heat generated by the heating mechanism 2 can flow to the bottom, the outside and the top of the culture dish respectively, so that under control, the effect of heating different positions of the culture dish can be achieved. By setting the arc-shaped pipe 41, it can be connected to the transfer box 27, so that the hot air generated by heating the water tank 21 can enter the inner cavity of the arc-shaped pipe 41 and wrap the culture dish. By setting the semi-circular pipe 43, it can rotate in the inner cavity of the arc-shaped pipe 41, and then adjust the position of the exhaust port 45 to achieve the effect of adjusting the direction of the hot air flow. On the upper surface of the placement rack 3, there is a culture dish mechanism 6. The culture dish mechanism 6 includes a culture dish bottom shell 61 and a culture dish cover 63. The culture dish bottom shell 61 is located on the upper surface of the placement rack 3. The culture dish cover 63 is movably connected to the opening of the culture dish bottom shell 61. By setting the culture dish mechanism 6, the stem cells to be cultivated can be placed, and under the action of the temperature adjustment mechanism 4 and the heating mechanism 2, the stem cells can be at an appropriate temperature. By setting the culture dish bottom shell 61 and the culture dish cover 63, the stem cells to be cultivated can be placed.

[0034] A one-way valve 22 penetrates through the top of the outer side surface of the water tank 21. One end of the one-way valve 22 is fixedly connected to a water inlet pipe 23. The heating mechanism 2 further includes a high-voltage DC power supply 24, and the high-voltage DC power supply 24 is fixedly connected to the inner wall of the base 1. The output end of the high-voltage DC power supply 24 is connected to a heating plate 25 through a wire. The heating plate 25 is tightly attached to the lower surface of the water tank 21. By providing the one-way valve 22, water can only enter the inner cavity of the water tank 21 through the water inlet pipe 23, and the water or air in the inner cavity of the water tank 21 will not be discharged. By providing the high-voltage DC power supply 24, during operation, current can flow to the heating plate 25, so that the resistance wire inside the heating plate 25 generates heat, thereby heating the water tank 21. A cushion strip 5 is fixedly connected to the upper surface of the placement rack 3. The cushion strip 5 is in pressing fit with the lower surface of the bottom shell 61 of the culture dish. A connecting plate 8 is fixedly connected to the outer surface of the placement rack 3. One end of the connecting plate 8 is fixedly connected to a limiting frame 9. A rotating rod 10 is rotatably connected to the inner cavity of the limiting frame 9. An anti-slip wheel 11 is fixedly connected to the outer surface of the rotating rod 10. The anti-slip wheel 11 is made of rubber and has many anti-slip grooves on its outer surface. By providing the cushion strip 5, the bottom of the bottom shell 61 of the culture dish can be protected to prevent the bottom of the glass bottom shell 61 of the culture dish from being damaged by knocking. By providing the limiting frame 9, the rotating rod 10 can be limited, so that the rotating rod 10 can rotate stably in the inner cavity of the limiting frame 9. By providing the anti-slip wheel 11, the bottom shell 61 of the culture dish can be supported, so that when the bottom shell 61 of the culture dish rotates, it can rotate more stably.

[0035] The second embodiment is as Figures 6 - 8As shown, a track groove 42 is formed on the outer side surface of the arc-shaped tube 41. A handle 44 is fixedly connected to the outer side surface of the semi-circular tube 43. The handle 44 is slidably connected to the track groove 42 formed on the outer side surface of the arc-shaped tube 41. The exhaust port 45 is movably connected to the ventilation hole formed on the upper surface of the arc-shaped tube 41. A sealing ring 46 is fixedly connected to the upper surface of the exhaust port 45. By providing the track groove 42, the handle 44 can be limited, enabling the handle 44 to move stably at the track groove 42 formed on the outer side surface of the arc-shaped tube 41. By providing the handle 44, the movement of the semi-circular tube 43 can be facilitated. By providing the sealing ring 46, the sealing performance at the top of the exhaust port 45 can be enhanced. Connection boxes 47 are fixedly connected to the ventilation holes formed on the upper surface of the arc-shaped tube 41. An exhaust hood 48 penetrates through the outer side surface of the connection box 47. The exhaust hood 48 is made of rubber material. The opening of the exhaust hood 48 is frictionally adapted to the outer surface of the bottom shell 61 of the culture dish. A first fixing plate 49 is fixedly connected to the inner wall of the connection box 47. A sealing plate 410 is fixedly connected to the outer surface of the first fixing plate 49. The sealing plate 410 is press-fitted with the sealing ring 46. By providing the connection box 47, the ventilation holes formed on the upper surface of the arc-shaped tube 41 can be wrapped. By providing the exhaust hood 48, when the exhaust port 45 is not in contact with the sealing plate 410, the hot air discharged from the arc-shaped tube 41 can be discharged to the outer side surface of the bottom shell 61 of the culture dish through the exhaust hood 48, thereby increasing the temperature of the outer wall of the bottom shell 61 of the culture dish. By providing the sealing plate 410, the top of the exhaust port 45 can be sealed, so that the hot air will not be discharged from the exhaust port 45 but will always be in the inner cavity of the arc-shaped tube 41, thereby increasing the temperature of the arc-shaped tube 41 and further heating the periphery of the bottom shell 61 of the culture dish.

[0036] A second fixing plate 411 is fixedly connected to the side of the connection box 47 away from the first fixing plate 49. A ventilation ring 412 is fixedly connected to the outer surface of the second fixing plate 411. The ventilation ring 412 is press-fitted with the upper surface of the sealing ring 46. A straight tube 413 is fixedly connected to the upper surface of the ventilation ring 412. The straight tube 413 penetrates through the connection box 47. A connecting tube 414 is fixedly connected to the end of the straight tube 413 that penetrates through the connection box 47. By providing the ventilation ring 412, when the semi-circular tube 43 rotates and the exhaust port 45 comes into contact with the lower surface of the ventilation ring 412, the hot steam in the inner cavity of the arc-shaped tube 41 can enter the inner cavity of the straight tube 413 through the ventilation ring 412 and finally be discharged from the connecting tube 414.

[0037] The third embodiment is as Figures 9 - 11As shown, a first telescopic tube 64 is fixedly connected to the upper surface of the culture dish cover 63. The first telescopic tube 64 is made of rubber and has a certain deformation ability. The upper surface of the first telescopic tube 64 is fixedly connected to a top box 65. One end of the connecting tube 414 far from the straight tube 413 penetrates through the top box 65. A hexagonal frame 62 is fixedly connected to the center of the lower surface of the culture dish bottom shell 61. By setting the first telescopic tube 64, a certain deformation can be generated, so that the top box 65 can drive the culture dish cover 63 to move up and down, which is convenient for the culture dish cover 63 to move up and down to close the opening of the culture dish bottom shell 61 after the culture dish bottom shell 61 is placed on the upper surface of the placement rack 3.

[0038] A rotating mechanism 7 is provided on the upper surface of the water tank 21. The rotating mechanism 7 includes a second telescopic tube 71. The second telescopic tube 71 penetrates through the center of the upper surface of the water tank 21. The second telescopic tube 71 is made of rubber material and has a certain deformation ability. The top end of the second telescopic tube 71 is fixedly connected with a support rod 78. The end of the support rod 78 is fixedly connected with a limiting ring 76. By providing the rotating mechanism 7, when it is necessary to rotate the bottom shell 61 of the petri dish, the steam generated by the water tank 21 can be used to control the stable rotation of the bottom shell 61 of the petri dish, so that the temperature in the inner cavity of the bottom shell 61 of the petri dish can rise stably. By providing the second telescopic tube 71, it can produce an up and down movement effect under extrusion and stretching. The outer surface of the limiting ring 76 is fixedly connected with a sliding frame 75. The number of the sliding frames 75 is three, and the three sliding frames 75 are evenly distributed. The ends of the three sliding frames 75 are all slidably connected with a limiting rod 74. The top end of the limiting rod 74 is fixedly connected to the lower surface of the placing rack 3. By providing the limiting rod 74, the sliding frame 75 can be limited, so that the sliding frame 75 can produce a vertical up and down movement effect, and then the position of the limiting ring 76 can be adjusted. The bottom of the inner wall of the second telescopic tube 71 is fixedly connected with a circular plate 72. The side of the upper surface of the circular plate 72 penetrates through a pressurizing port 73. The inner cavity of the limiting ring 76 is rotatably connected with a rotating frame 77. The upper surface of the rotating frame 77 is fixedly connected with a hexagonal prism 711. The hexagonal prism 711 is frictionally adapted to the inner wall of the hexagonal frame 62. The lower surface of the rotating frame 77 is fixedly connected with a rotating column 79. The outer surface of the rotating column 79 is fixedly connected with an inclined plate 710. The inclined plate 710 is located directly above the pressurizing port 73. The number of the inclined plates 710 is several, and the several inclined plates 710 are evenly distributed. By providing the pressurizing port 73, when the water in the inner cavity of the water tank 21 is heated to generate water vapor, the water vapor can quickly discharge from the pressurizing port 73, so that the water vapor can squeeze the inclined plate 710. When the inclined plate 710 is impacted by the airflow, the rotating column 79 will drive the rotating frame 77 to rotate, and then the hexagonal prism 711 will rotate, and then drive the hexagonal frame 62 to rotate, and finally make the bottom shell 61 of the petri dish rotate stably.

[0039] Working principle: During use, the operator pulls the petri dish cover 63 and makes the first telescopic tube 64 stretch and contract, so that the bottom shell 61 of the petri dish can be removed. Then, the diluted stem cell suspension is slowly added to the bottom shell 61 of the petri dish gently to avoid generating bubbles. If it is adherent stem cells, after inoculation, the petri dish should be gently shaken to make the cells evenly distributed at the bottom of the inner cavity of the bottom shell 61 of the petri dish. For suspended stem cells, attention should be paid to keeping the cell suspension in a uniform state. The petri dish can be placed in a cell culture incubator and left standing for a period of time to allow the cells to adapt to the new environment.

[0040] Then, the culture dish bottom shell 61 is placed on the upper surface of the placement rack 3, and then the culture dish cover 63 is pressed to seal the culture dish bottom shell 61. Then, water is poured into the inner cavity of the water tank 21 through the water inlet pipe 23. Then, the high-voltage DC power supply 24 is connected to the power supply and the switch is turned on, so that the resistor in the inner cavity of the heating plate 25 is connected to the flow and finally generates heat, thereby increasing the water temperature in the inner cavity of the water tank 21. During the stem cell culture process, the water temperature in the inner cavity of the water tank 21 is controlled not to exceed 60 degrees Celsius, and the hot air will enter the arc tube 41 through the ventilation pipe 26. When the temperature of the periphery of the culture dish bottom shell 61 needs to be evenly increased, the operator moves the handle 44 and causes the semicircular tube 43 to rotate in the inner cavity of the arc tube 41, so that the exhaust port 45 is located between the sealing plate 410 and the air-permeable ring 412. At this time, the hot air will flow to the inner cavities of the exhaust hood 48 and the straight tube 413 respectively, and finally the hot air will contact the outer surface of the culture dish bottom shell 61, and the hot air of the connecting tube 414 will enter the inner cavity of the first telescopic tube 64, and finally the hot air will contact the culture dish cover 63, so that the culture dish is evenly heated;

[0041] When only the bottom of the culture dish needs to be heated, the operator places the exhaust port 45 at the sealing plate 410 to prevent the hot air from being discharged from the inner cavity of the arc tube 41, thereby heating the bottom of the culture dish bottom shell 61;

[0042] When the culture dish needs to be disinfected, the operator moves the sliding frame 75, thereby inserting the hexagonal prism 711 into the inner cavity of the hexagonal frame 62, and then controls the output current of the high-voltage DC power supply 24, and increases the temperature of the water flow in the inner cavity of the water tank 21 and eventually generates a large amount of water vapor. At this time, the water vapor will be ejected from the boost port 73, and press the inclined plate 710, thereby causing the rotating column 79 and the rotating frame 77 to rotate, and finally causing the hexagonal prism 711 to drive the culture dish bottom shell 61 to rotate, and the water vapor will be ejected from the opening of the second telescopic tube 71, and evenly contact the bottom of the culture dish bottom shell 61, so as to kill the internal materials.

[0043] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A stem cell culture dish, comprising a base, characterized in that: A heating mechanism is arranged on the upper surface of the base, and the heating mechanism comprises a water tank, which is fixedly connected to the upper surface of the base, and a ventilation pipe is passed through the outer side of the water tank, and a transfer box is fixedly connected to the end of the ventilation pipe away from the water tank; a temperature regulating mechanism is arranged on the side of the transfer box away from the ventilation pipe, and the temperature regulating mechanism comprises an arc tube, which passes through the side of the transfer box away from the ventilation pipe, and a placement rack is fixedly connected to the lower surface of the arc tube, and a plurality of air holes are opened on the upper surface of the arc tube, and a semicircular tube is slidably connected to the inner cavity of the arc tube, and an exhaust port is passed through the upper surface of the semicircular tube, and the number of the exhaust ports is consistent with the number of the air holes opened on the upper surface of the arc tube; a culture dish mechanism is arranged on the upper surface of the placement rack, and the culture dish mechanism comprises a culture dish bottom shell and a culture dish cover, the culture dish bottom shell is located on the upper surface of the placement rack, and the culture dish cover is movably connected to the opening of the culture dish bottom shell.

2. A stem cell culture dish according to claim 1, characterized in that: A one-way valve penetrates the top of the outer side of the water tank, and the end of the one-way valve is fixedly connected to a water inlet pipe. The heating mechanism also includes a high-voltage DC power supply, which is fixedly connected to the inner wall of the base. The output end of the high-voltage DC power supply is connected to a heating plate through a wire, and the heating plate is tightly attached to the lower surface of the water tank.

3. A stem cell culture dish according to claim 1, characterized in that: A pad is fixedly connected to the upper surface of the placement rack, and the pad is squeezed and adapted to the lower surface of the bottom shell of the culture dish. A connecting plate is fixedly connected to the outer surface of the placement rack, and the end of the connecting plate is fixedly connected to a limiting frame. A rotating rod is rotatably connected to the inner cavity of the limiting frame, and an anti-skid wheel is fixedly connected to the outer surface of the rotating rod. The anti-skid wheel is made of rubber and has many anti-skid grooves on the outer surface.

4. A stem cell culture dish according to claim 3, characterized in that: A track groove is provided on the outer side of the arc tube, a handle is fixedly connected to the outer side of the semicircular tube, the handle is slidably connected to the track groove provided on the outer side of the arc tube, the exhaust port is movably connected to the air vent provided on the upper surface of the arc tube, and a sealing ring is fixedly connected to the upper surface of the exhaust port.

5. A stem cell culture dish according to claim 4, characterized in that: The air holes on the upper surface of the arc tube are fixedly connected to a connection box, an exhaust hood is passed through the outer side of the connection box, the exhaust hood is made of rubber, the opening of the exhaust hood is frictionally matched with the outer surface of the bottom shell of the culture dish, and a first fixing plate is fixedly connected to the inner wall of the connection box, a sealing plate is fixedly connected to the outer surface of the first fixing plate, and the sealing plate is squeezed and matched with the sealing ring.

6. A stem cell culture dish according to claim 5, characterized in that: A second fixed plate is fixedly connected to the side of the connection box away from the first fixed plate, a breathable ring is fixedly connected to the outer surface of the second fixed plate, the breathable ring is extruded and adapted to the upper surface of the sealing ring, a straight pipe is fixedly connected to the upper surface of the breathable ring, the straight pipe passes through the connection box, and one end of the straight pipe passing through the connection box is fixedly connected to a connecting pipe.

7. A stem cell culture dish according to claim 6, characterized in that: A first telescopic tube is fixedly connected to the upper surface of the culture dish cover. The first telescopic tube is made of rubber and has a certain deformation ability. A top box is fixedly connected to the upper surface of the first telescopic tube. The end of the connecting tube away from the straight tube passes through the top box. A hexagonal frame is fixedly connected to the axis of the lower surface of the culture dish bottom shell.

8. A stem cell culture dish according to claim 7, characterized in that: A rotating mechanism is provided on the upper surface of the water tank, and the rotating mechanism includes a second telescopic tube, which passes through the axis of the upper surface of the water tank. The second telescopic tube is made of rubber and has a certain deformation ability. The top end of the second telescopic tube is fixedly connected to a support rod, and the end of the support rod is fixedly connected to a limiting ring.

9. The stem cell culture dish according to claim 7, characterized in that: The outer surface of the limit ring is fixedly connected with a sliding frame, the number of the sliding frames is three, and the three sliding frames are evenly distributed, and the ends of the three sliding frames are slidably connected to the limit rod, and the top end of the limit rod is fixedly connected to the lower surface of the placement frame.

10. A stem cell culture dish according to claim 9, characterized in that: A circular plate is fixedly connected to the bottom of the inner wall of the second telescopic tube, and the side of the upper surface of the circular plate passes through the boost port. A rotating frame is rotatably connected to the inner cavity of the limiting ring, and a hexagonal column is fixedly connected to the upper surface of the rotating frame. The hexagonal column is frictionally fitted with the inner wall of the hexagonal frame, and a rotating column is fixedly connected to the lower surface of the rotating frame. An inclined plate is fixedly connected to the outer surface of the rotating column, and the inclined plate is located directly above the boost port. There are several inclined plates, and the several inclined plates are evenly distributed.

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