Bactericidal composition and application thereof to prevention and treatment of fusarium diseases

Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a sterilization composition and application thereof to prevention and treatment of fusarium diseases, the sterilization composition comprises an active component A and an active component B. The active component A is cyclocutrifluram, and the active component B is feneptamido quin; the mass ratio of the active component A to the active component B is (1: 45)-(40: 1). The bactericidal composition disclosed by the invention has an obvious synergistic effect on various plant diseases caused by fusarium pathogenic bacteria, can effectively reduce the occurrence and harm of fusarium diseases, and ensures agricultural production and food safety.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide sterilization and discloses a sterilization composition and an application thereof in preventing and controlling fusarium diseases. Background Art

[0002] Fusarium fungi are important pathogens that cause a variety of soil-borne diseases of crops. They can cause a variety of plant diseases in agricultural production, such as wheat ergot, wheat stem rot, rice seedling blight and other diseases. When the diseases occur seriously, they can cause significant economic losses to agricultural production.

[0003] Wheat head rot, also known as wheat head rot, is a climatic disease of wheat caused by the fungal pathogen Fusarium graminearum. Fusarium graminearum exists in the soil or plant diseased residues in the form of asexual conidia. When the climatic conditions are suitable, it enters the plant body and infects the wheat ears. The disease is mainly manifested by the appearance of a red mold layer on the wheat ears. This disease occurs in wheat-producing areas around the world. Once it occurs, it will lead to a large-scale reduction in wheat production, and in severe cases, it will even lead to a total loss of wheat production in wheat-growing areas. In addition, Fusarium graminearum will produce a variety of toxic substances in the plant host, including fusarin C, aurein, zearalenone, deoxynivalenol and their derivatives. The production of these toxins will greatly reduce the yield and quality of wheat crops, and will seriously threaten the health of humans and animals.

[0004] Wheat crown rot (FCR) is a soil-borne fungal disease caused by Fusarium fungi that infects the base of wheat stems and can cause damage throughout the entire growth period. In the early stages of the disease, seeds cannot germinate normally, roots rot, and leaves turn yellow; as the disease progresses, the base of the stem turns brown and is easy to break, and red or white mold forms at the nodes, eventually causing white ears of wheat plants, unfilled grains or even seedless grains, greatly affecting wheat yields. In addition, after being infected by pathogens, the plant will produce a series of biologically active secondary metabolic toxins, mainly trichothecenes. Ingestion of wheat containing such toxins will pose a serious threat to the life and health of humans and other mammals.

[0005] Rice seedling blight, also known as leggy growth disease, is a seed-borne disease caused by the fungus Fusarium moniliforme of the subphylum Ascomycetes. The bacteria carried by rice seeds usually invade through wounds, then lurk in the seeds and become diseased when conditions are right. In addition to seeds carrying pathogens, soil and diseased plants can also transmit the disease. Rice seedling blight can occur throughout the entire growth period of rice. The specific symptoms are tall and thin plants, pale leaves, abnormal elongation of internodes and the growth of adventitious roots. In the late stage of the disease, the entire plant may turn yellow and die, and the fruit set rate is reduced.

[0006] Regarding the diseases caused by the above-mentioned Fusarium fungi, the present invention rationally combines cyclobutrifluram and feneptamidoquin to study its control effect, determines the appropriate ratios of single agents and compound preparations that have a synergistic effect on the above diseases through indoor bioactivity determination tests, and further verifies its control effect in the field environment through field efficacy tests, providing a new implementation scheme for the comprehensive control of Fusarium diseases in agriculture. Summary of the Invention

[0007] Based on the above, the present invention provides a bactericidal composition, which has an obvious synergistic effect on various Fusarium diseases caused by pathogenic Fusarium fungi, can effectively reduce the occurrence and harm of Fusarium diseases, and ensure agricultural production and food safety.

[0008] To achieve the above object, the present invention adopts the following technical solution: a bactericidal composition, the bactericidal composition contains active ingredient A and active ingredient B, the active ingredient A is cyclobutrifluram, and the active ingredient B is feneptamidoquin; the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 40:1, or any value within the above numerical range.

[0009] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 30:1, or any value within the above numerical range.

[0010] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 27:1, or any value within the above numerical range.

[0011] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:1, or any value within the above numerical range.

[0012] Further, the total weight of the bactericidal composition is calculated as 100 wt%, and the active ingredient accounts for 0.5% to 70% of the total weight of the bactericidal composition, or any value within the above numerical range.

[0013] Further, in addition to the active ingredient, the bactericidal composition also contains agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.

[0014] Further, the bactericidal composition can be prepared into any preparation dosage form acceptable in the pesticide field, and the preparation dosage form is selected from liquid preparations or solid preparations.

[0015] Further, the liquid preparation is a suspending agent, emulsifiable concentrate, emulsifiable oil in water, microemulsion, dispersible oil suspension, seed treatment suspension, and the solid preparation is a water dispersible granule, wettable powder.

[0016] The present invention also discloses the use of the bactericidal composition and its preparation as described above for preventing and treating Fusarium diseases. The pathogenic bacteria causing Fusarium diseases are: Fusarium graminearum, Pseudograminearum, Fusarium oxysporum, Fusarium solani, Fusarium moniliforme, Fusarium culmorum, Fusarium equiseti, Fusarium roseum, Fusarium tricinctum, Fusarium avenaceum, Fusarium proliferatum.

[0017] Further, the pathogenic bacteria are Fusarium graminearum, Pseudograminearum, and Fusarium moniliforme.

[0018] The beneficial effects of the present invention are as follows:

[0019] 1) The bactericidal composition of the present invention is compounded with compounds having different action mechanisms, and the most suitable ratio is screened according to the effects on different Fusarium fungi and target plants. The synergistic effect is significant, the development of drug resistance is delayed, and the drug life cycle is extended.

[0020] 2) The bactericidal composition of the present invention has a low dosage, good control effect, and is safe for target crops, meeting the actual control requirements of diseases. Specific Embodiments

[0021] In order to make the purpose, advantages and technical solutions of the present invention clearer, the following preparation examples and specific examples of the preparation are used to explain the technical solutions of the present invention. However, the protection scope of the present invention should not be limited by the specific embodiments described herein.

[0022] Preparation Examples and Preparation Methods of Preparations:

[0023] 1. Seed treatment suspension: According to the formula ratio, the active ingredient, auxiliary agent, and water are mixed evenly by high-shear mixing and stirring, and then sanded for 2.5 h by a sand mill to make the average particle size reach 1-5 microns, and the seed treatment suspension can be obtained.

[0024] 2. Suspending agent: According to the formula ratio, the active ingredient, surfactant, and other functional auxiliary agents are placed in a reaction kettle in turn, mixed evenly with water, and then subjected to high-speed shearing, wet sanding, and finally homogenized and filtered to obtain the suspending agent product.

[0025] 3. Microemulsion: According to the formula ratio, the active ingredient, solvent, emulsifier, etc. are mixed evenly to obtain the oil phase, the antifreeze and water are mixed evenly to obtain the water phase, the oil phase is added to the water phase under stirring and stirred evenly, and then sheared for 10 min. After adding the silicone defoamer and stirring evenly, small droplets with oil phase particles of 0.01 - 0.1 microns are obtained, and thus the microemulsion of the present invention is prepared.

[0026] 4. Emulsion in water: According to the formula ratio, the active ingredient is dissolved in the solvent and the emulsifier is added to dissolve it into a uniform oil phase. Deionized water, antifreeze, etc. are mixed together to form a uniform water phase. Under high-speed shearing, the oil phase is added to the water phase, and finally a thickener and a defoamer are added to form a well-dispersed emulsion in water product.

[0027] 4. Emulsifiable concentrate: According to the formula ratio, the metered active ingredient, solvent, and cosolvent are added to the mixing kettle and stirred to dissolve them. Then the emulsifier is added, and the remaining solvent is used to make up the balance. After stirring evenly in the stirring kettle and filtering, the emulsifiable concentrate required by the present invention is obtained.

[0028] 5. Water dispersible granule: According to the formula ratio, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, and they are mixed. After being pulverized by air flow, 10 - 25% of water is added, and then it is kneaded, granulated, dried, and sieved to obtain the water dispersible granule product; or the pulverized powder is sprayed with water, granulated, and dried in a fluidized bed granulator, and then sieved to obtain the product.

[0029] 6. Wettable powder: According to the formula ratio, the active ingredient, dispersant, wetting agent, and filler are mixed, and they are stirred evenly in the stirring kettle. After being pulverized and mixed evenly by an air flow pulverizer for multiple times, the wettable powder of the composition of the present invention can be prepared.

[0030] Preparation Example 1: 20% cyclobutrifluram·feneptamidoquin seed treatment suspension (1:9)

[0031] Formulation composition: 2% cyclobutrifluram, 18% feneptamidoquin, 1% triphenylethylphenol polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether phosphate, 2% sodium lignosulfonate, 2% polyacrylic acid emulsion, 0.25% xanthan gum, 5% rose pigment, 5% glycerol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water is used to make up the balance.

[0032] Preparation Example 2: 15% cyclobutrifluram·feneptamidoquin suspension (1:1)

[0033] Formulation composition: 7.5% cyclobutrifluram, 7.5% feneptamidoquin, 2% sodium octylphenol polyoxyethylene ether sulfonate, 2% EO-PO block copolymer, 2% alkyl polyoxyethylene ether sulfonate, 1% sodium polycarboxylate, 0.5% silicone defoamer, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.5% sodium benzoate, deionized water to make up the balance.

[0034] Preparation Example 3: 12% cyclobutrifluram·feneptamidoquin aqueous emulsion (3:1)

[0035] Formulation composition: 9% cyclobutrifluram, 3% feneptamidoquin, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 3% arylphenol polyoxyethylene ether phosphate, 15% cyclohexanone, 0.25% xanthan gum, 5% ethylene glycol, 1% urea, 0.1% sodium sorbate, 0.5% silicone defoamer, deionized water to make up the balance.

[0036] Preparation Example 4: 15% cyclobutrifluram·feneptamidoquin emulsifiable concentrate (3:2)

[0037] Formulation composition: 9% cyclobutrifluram, 6% feneptamidoquin, 15% DMF, 10% phenethylphenol polyoxyethylene polyoxypropylene ether, 2% calcium dodecylbenzenesulfonate, 15% propylene carbonate, xylene to make up the balance.

[0038] Preparation Example 5: 30% cyclobutrifluram·feneptamidoquin water dispersible granule (5:1)

[0039] Formulation composition: 25% cyclobutriflura, 5% feneptamidoquin, 3% sodium dodecyl sulfate, 5% sulfosuccinate, 2% sodium polycarboxylate, 5% white sugar, kaolin to make up the balance.

[0040] Preparation Example 6: 28% cyclobutrifluram·feneptamidoquin wettable powder (6:1)

[0041] Formulation composition: 24% cyclobutrifluram, 4% feneptamidoquin, 2% sodium dodecyl sulfate, 3% fatty alcohol polyoxyethylene ether sulfate, 3% dioctyl sulfosuccinate, 10% kaolin, 8% white carbon black, bentonite to make up the balance.

[0042] Example 1: Indoor Bioactivity Test of the Compound of Cyclobutrifluram and Feneptamidoquin against Fusarium

[0043] Test Basis: The test refers to the standard NY / T 1156.2 - 2006, "Guidelines for Pesticide Bioassay in the Laboratory - Part 2: Fungicides - Petri Dish Method for Inhibiting the Mycelial Growth of Pathogenic Fungi".

[0044] Test Targets: Fusarium graminearum (wheat head blight), Fusarium moniliforme (rice bakanae disease), Fusarium pseudograminearum (wheat basal stalk rot).

[0045] Instruments and Equipment: High - pressure steam sterilizer, laminar flow hood, incubator, electro - thermal blast drying oven, ten - thousandth electronic balance, pipette, alcohol lamp, beaker, volumetric flask, Erlenmeyer flask, Petri dish, borer, inoculator, ruler, etc.

[0046] Preparation of Test Materials: Transfer the Fusarium preserved in the indoor refrigerator to the potato dextrose agar medium for activation and reserve.

[0047] Test Agents: Cyclobutrifluram technical, Feneptamidoquin technical.

[0048] Agent Preparation: Dissolve the above technical agents with a suitable solvent to make a high - concentration stock solution, and then dilute it with a 0.1% Tween 80 aqueous solution. Prepare single - agent stock solutions respectively, and design different ratios according to the purpose of compounding and the activity of the agents. Each single agent and the mixed agents of each group ratio are prepared into the required series of mass concentrations.

[0049] Test Repetition: For each concentration of the test agents, 4 Petri dishes are used, 1 Petri dish for each repetition, with a total of 4 repetitions. Use a 0.1% Tween 80 aqueous solution without the agent as the blank control.

[0050] Agent Treatment: Under sterile operating conditions, use a pipette to add 5 mL of the liquid medicine with different concentrations to the accurately calibrated sterile Erlenmeyer flask respectively, then add the medium melted and cooled to an appropriate temperature to the Erlenmeyer flask, shake well and pour an equal amount into 4 Petri dishes to make the corresponding concentration of PDA plates containing the agent.

[0051] Inoculation: Cut the fungal cake from the edge of the pre - cultured Fusarium colony with a sterile borer under sterile conditions, and use an inoculator to inoculate the fungal cake in the center of the agent - containing plate, cover the lid, and place it in a constant - temperature incubator at 25℃ for dark culture.

[0052] Data investigation: Conduct experimental investigation when the colonies in the control treatment grow to 2 / 3 to 4 / 5 of the diameter of the petri dish. Measure the colony diameter (cm) with a ruler. Measure the diameter of each colony once using the cross-cross method and take the average value.

[0053] Data statistics and analysis: According to the investigation results, calculate the mycelial growth inhibition rate of each treatment concentration on the tested target bacteria, with the unit of percentage (%). Keep the calculation result to two decimal places.

[0054] D = D1 - D2

[0055] In the formula:

[0056] D - - Colony growth diameter;

[0057] D1 - - Colony diameter;

[0058] D2 - - Disc diameter.

[0059]

[0060] In the formula:

[0061] I - - Mycelial growth inhibition rate;

[0062] D0 - - Colony growth diameter of the blank control;

[0063] D T - - Colony growth diameter of the medicament treatment.

[0064] Analyze with the DPS statistical analysis system to obtain the toxicity regression line and EC 50 value, and evaluate the activity of the tested medicaments on the biological test materials.

[0065] Sun Yunpei method: Evaluate the synergistic effect of medicament mixtures according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the mixture ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0066] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0067]

[0068] In the formula:

[0069] ATI - - Measured toxicity index of the mixture;

[0070] S - - EC of the standard medicament 50 , with the unit of milligrams per liter (mg / L);

[0071] M - - EC of the mixture 50 , with the unit of milligrams per liter (mg / L).

[0072] TTI = TI A × P A + TI B × P B

[0073] In the formula:

[0074] TTI—theoretical toxicity index of the mixture

[0075] TI A —toxicity index of chemical A

[0076] P A —percentage content of chemical A in the mixture, in percentage (%)

[0077] TI B —toxicity index of chemical B

[0078] P B —percentage content of chemical B in the mixture, in percentage (%).

[0079]

[0080] In the formula:

[0081] CTC—coefficient of co-toxicity

[0082] ATI—measured toxicity index of the mixture

[0083] TTI—theoretical toxicity index of the mixture

[0084] The test results are shown in the following table:

[0085] Table 1 Test results of indoor biological activity determination of the combination of cyclobutrifluram and feneptamidoquin against Fusarium graminearum

[0086]

[0087] It can be seen from the results of indoor experiments that the compound of cyclobutrifluram and feneptamidoquin has a relatively excellent control effect on the mycelial growth of Fusarium graminearum at appropriate mass ratios. When the mass ratio of cyclobutrifluram to feneptamidoquin is 1:30 - 27:1, the co-toxicity coefficient against Fusarium graminearum is greater than 120, showing a synergistic effect; when the mass ratio of cyclobutrifluram to feneptamidoquin is 1:15 - 18:1, the co-toxicity coefficient against Fusarium graminearum is greater than 130, with an obvious synergistic effect; when the mass ratio of cyclobutrifluram to feneptamidoquin is 1:15 - 9:1, the co-toxicity coefficient against Fusarium graminearum is greater than 140, with a significant synergistic effect.

[0088] Table 2 Results of indoor bioactivity determination test of the compound of cyclobutrifluram and feneptamidoquin against Fusarium moniliforme

[0089]

[0090] It can be seen from the results of indoor experiments that the compound of cyclobutrifluram and feneptamidoquin has a relatively excellent control effect on the mycelial growth of Fusarium moniliforme at appropriate mass ratios. When the mass ratio of cyclobutrifluram to feneptamidoquin is 1:45 - 30:1, the co-toxicity coefficient against Fusarium moniliforme is greater than 120, showing a synergistic effect; when the mass ratio of cyclobutrifluram to feneptamidoquin is 1:25 - 20:1, the co-toxicity coefficient against Fusarium moniliforme is greater than 140, with an obvious synergistic effect; when the mass ratio of cyclobutrifluram to feneptamidoquin is 1:15 - 20:1, the co-toxicity coefficient against Fusarium moniliforme is greater than 150, with a significant synergistic effect.

[0091] Table 3 Results of indoor bioactivity determination test of the compound of cyclobutrifluram and feneptamidoquin against Pseudogymnoascus destructans

[0092]

[0093] It can be seen from the results of laboratory tests that the compounding of cyclobutrifluram and feneptamidoquin has an excellent control effect on the mycelial growth of Fusarium pseudograminearum at an appropriate mass ratio. When the mass ratio of cyclobutrifluram to feneptamidoquin is 1:20 to 36:1, the co-toxicity coefficient against Fusarium pseudograminearum is greater than 120, showing a synergistic effect; when the mass ratio of cyclobutrifluram to feneptamidoquin is 1:12 to 30:1, the co-toxicity coefficient against Fusarium pseudograminearum is greater than 140, with an obvious synergistic effect; when the mass ratio of cyclobutrifluram to feneptamidoquin is 1:6 to 24:1, the co-toxicity coefficient against Fusarium pseudograminearum is greater than 150, with a significant synergistic effect.

[0094] Example 2: Field efficacy test for controlling wheat scab

[0095] Test site: Zishu Village, Linhuai Town, Huoqiu County, Lu'an City, Anhui Province. The soil fertility is medium, the previous crop is rice, the test field is flat, the plot is neat, the fertility is medium, and the irrigation and drainage are convenient, meeting the local scientific agricultural practices.

[0096] Test target: Wheat scab.

[0097] Test crop: Wheat.

[0098] Test design: A total of 6 treatments were set up in the test, including 5 pesticide application treatments and 1 blank control. The test plots were arranged in a randomized block design, with each treatment replicated 4 times. The area of each test plot was 30 m 2 .

[0099] Test method: The pesticide was applied once using a Gongnong-16 type manual knapsack sprayer during the early flowering stage after the wheat reached full heading. The investigation was carried out during the filling stage of the wheat. When investigating, 5 sampling points were taken in each plot, and 100 ears were investigated at each point. The percentage of the area of the withered ear in the total ear area was used for grading, and the number of diseased ears at each level and the total number of ears were recorded.

[0100] Grading method:

[0101] Grade 0: The whole ear is disease-free;

[0102] Grade 1: The withered ear area accounts for less than 1 / 4 of the whole ear area;

[0103] Grade 3: The withered ear area accounts for 1 / 4 to 1 / 2 of the whole ear area;

[0104] Grade 5: The withered ear area accounts for 1 / 2 to 3 / 4 of the whole ear area;

[0105] Grade 7: The withered ear area accounts for more than 3 / 4 of the whole ear area.

[0106] Pharmacodynamic calculation method:

[0107]

[0108] Pharmacodynamic test results and analysis:

[0109] Table 4 Field control efficacy test results of wheat scab

[0110]

[0111] It can be seen from the field pharmacodynamic test results that the compounding of Cyclobutrifluram and feneptamidoquin in a reasonable ratio has a good control effect on wheat scab.

[0112] Example 3: Field control efficacy test of wheat basal stalk rot

[0113] Test site: The test was carried out in the wheat planting area of Xubao Village, Wude Town, Wen County, Jiaozuo City, Henan Province. The test plot was flat, rich in organic matter, with the previous crop being corn, and wheat basal stalk rot occurred seriously all year round.

[0114] Test object: Wheat basal stalk rot.

[0115] Test crop: Wheat (Yannong 988).

[0116] Test design: A total of 5 chemical treatments and 1 clear water blank control were set in the test. The field plots were arranged in a randomized block design, with each plot area of 50m 2 , and each treatment was repeated 4 times. Spraying was carried out at the wheat green-reverting stage, and the spraying equipment was a knapsack electric sprayer, with the water consumption of 60L / 667m 2 . Each treatment area and the blank control area were managed simultaneously.

[0117] Field control efficacy investigation: The investigation of wheat basal stalk rot was carried out at the wheat milk-ripe stage. Five points were sampled diagonally in each plot, and 100 plants were investigated at each point. The disease degree of wheat was classified and recorded according to the following classification method, and the disease index and control effect were calculated.

[0118] The disease was classified according to the following criteria:

[0119] Grade 0: The whole plant has no browning symptoms;

[0120] Grade 1: Browning phenomenon occurs at the roots;

[0121] Grade 3: Browning and rotting phenomenon occurs at the first stem node above the ground;

[0122] Grade 5: Browning and rotting phenomenon occurs at the second stem node above the ground;

[0123] Grade 7: The lesion exceeds the second stem node, but there is no white ear;

[0124] Grade 9: Lesions exceed the second stem node, with white ears.

[0125] Calculation formulas for disease index and control effect:

[0126]

[0127] Test results and analysis:

[0128] Table 5 Test results of field control of wheat basal stalk rot

[0129] Name of medicament <![CDATA[Active ingredient dosage (g / hm 2 )]]> Disease index Control effect (%) 15% cyclobutrifluram·feneptamidoquin EC (3:2) 50 1.61 91.02 30% cyclobutrifluram·feneptamidoquin WG (5:1) 50 1.85 89.66 28% cyclobutrifluram·feneptamidoquin WP (6:1) 50 2.40 86.58 15% Cyclobutrifluram EC 60 5.17 71.08 20% feneptamidoquin SC 100 5.71 68.10 Blank control / 17.88 -

[0130] The results of control effect investigation (Table 5) showed that among the treatments with various agents, the treatment with the compound preparation 15% cyclobutrifluram·feneptamidoquin EC (3:2) had the highest control effect. The disease index control effects of the compound preparation on basal stalk rot at the milk ripening stage of wheat were 91.02%, 89.66%, and 86.58% respectively, which were significantly higher than those of other single-agent treatments.

[0131] In summary, through indoor toxicity determination and field efficacy tests, it can be seen that the bactericidal composition of the present invention has good control effects on Fusarium, is safe for target crops, has significant control effects, is superior to single agents in delaying the generation of drug resistance and prolonging the persistence, and has obvious yield protection effects.

[0132] Although this application describes specific embodiments in detail with the help of examples, the disclosure of this application can adopt various modifications and replacement forms. However, it should be understood that the disclosure of this application is not limited to the specific forms disclosed. On the contrary, the disclosure of this application covers all modifications, equivalents, and replacement forms within the scope of the disclosure of this application, and the scope of this application is defined by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition, characterized in that: The bactericidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is cyclobutrifluram and the active ingredient B is feneptamidoquin; and the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 40:

1.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:45 to 30:

1.

3. The bactericidal composition according to claim 2, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:30 to 27:

1.

4. The bactericidal composition according to claim 3, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:

1.

5. The bactericidal composition according to claim 1, characterized in that The total weight of the bactericidal composition is 100 wt %, and the active ingredient accounts for 0.5% to 70% of the total weight of the bactericidal composition.

6. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicidal composition also contains auxiliary ingredients acceptable to pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.

7. The bactericidal composition according to claim 1, characterized in that The bactericidal composition can be prepared into any formulation acceptable in the pesticide field, and the formulation is selected from a liquid formulation or a solid formulation.

8. The bactericidal composition according to claim 7, characterized in that The liquid preparations are suspensions, emulsifiable concentrates, water emulsions, microemulsions, dispersible oil suspensions, and seed treatment suspensions; the solid preparations are water dispersible granules and wettable powders.

9. Use of the fungicidal composition according to any one of claims 1 to 8 for preventing and controlling Fusarium diseases, characterized in that: The pathogenic bacteria causing the fusarium disease are: Fusarium graminearum, false Fusarium graminearum, Fusarium oxysporum, Fusarium solani, Fusarium moniliforme, Fusarium flavum, Fusarium equisetum, Fusarium pink, Fusarium trispartium, Fusarium avenae, and Fusarium laminari.

10. The use according to claim 9, characterized in that The pathogenic bacteria are Fusarium graminearum, Pseudofusarium graminearum and Fusarium moniliforme.

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