Pesticide composition and application thereof in prevention and treatment of fusarium diseases

Abstract

The invention relates to a pesticide composition and application thereof to prevention and treatment of fusarium diseases, the pesticide composition comprises an active component A and an active component B. The active component A is Cyclobutrifluram, the active component B is Bifemetstrobin, and the mass ratio of the active component A to the active component B is 1: 40-22: 1. Under a certain mass ratio, the bactericidal effect of the pesticide composition is not simple superposition of the activity of each component, and the pesticide composition has high antibacterial activity on diseases caused by Fusarium, has obvious synergistic interaction, is safe to crops and has the effects of increasing yield and keeping harvest.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide fungicides, and specifically relates to a pesticide composition and its use for controlling diseases caused by Fusarium Background Art

[0002] Fusarium is an important pathogen causing many soil-borne and seed-borne diseases, such as wheat basal stalk rot, maize basal stalk rot, wheat scab, wheat root rot, etc. The pathogen compositions are similar, and the applicable chemical pesticides are also similar. In addition, the rotation cultivation system is generally adopted in most areas. Under this background, the long-term use of a single category of chemical pesticides not only easily causes the pesticide residues in the farmland environment to exceed the standard, leading to the decline of crop quality and endangering the ecological environment, but also easily causes the pathogens to develop drug resistance.

[0003] Cyclobutrifluram is an SDHI fungicide and nematicide with a phenylcyclobutylpyridine amide structure reported by Syngenta, CAS registration number: 1644251-74-0. It has broad-spectrum and high efficiency, can prevent the invasion of Fusarium and root-knot nematodes, and can be transported to the above-ground parts of plants to control early diseases.

[0004] Bifemetstrobin is a strobilurin fungicide developed by Sumitomo Chemical Co., Ltd., CAS registration number: 2454319-63-0, Chinese chemical name: methyl (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoate. It is an inhibitor of the outer Qo site (cyt b gene) of cytochrome bc1 (ubiquinol oxidase) in the respiratory chain mitochondrial complex III. Different from other strobilurin fungicides, an oxygen atom is inserted between the strobilurin and the benzene ring, and it has high activity against diseases such as rice blast, cucumber anthracnose, cucumber powdery mildew, wheat leaf rust, and maize gray leaf spot.

[0005] The present invention has conducted in-depth research on diseases caused by Fusarium. A large number of test results show that the mixture of the two provides a strong defense against a variety of Fusarium pathogens, has an obvious synergistic effect, and helps to delay the generation of resistance, which is of great significance for ensuring the safe production of crops. Summary of the Invention

[0006] Based on the above situation, the object of the present invention is to provide a pesticide composition, which is mainly used for controlling diseases caused by pathogenic bacteria of Fusarium in plants. The pesticide composition or its preparation can enhance the drug effect, reduce the dosage of the drug, and at the same time can extend the effective period and delay the development of drug resistance, and has high safety for crops.

[0007] This object is achieved through the following technical solutions: A pesticide composition comprising active ingredient A and active ingredient B, wherein the active ingredient A is Cyclobutrifluram and the active ingredient B is Bifemetstrobin;

[0008] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 22:1 or any value between the above values;

[0009] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 22:1;

[0010] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:1;

[0011] Further, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1 to 90 wt%;

[0012] Further, based on the total weight of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 5 to 80 wt%;

[0013] Further, the pesticide composition in addition to the active ingredients also contains agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers;

[0014] Further, the wetting agent is selected from one or more of alkyl benzene sulfonates, alkyl naphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, fatty alcohol polyoxyethylene ether sulfates, silkworm excrement, soap pod powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0015] Further, the dispersant is selected from one or more of lignin sulfonates, alkyl naphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0016] Further, the emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0017] Further, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica white; and / or

[0018] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0019] Further, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0020] Further, the defoamer is selected from one or more of C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0021] Further, the solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oils (such as soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0022] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0023] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica white, talc powder, montmorillonite, and starch; and / or

[0024] Further, the synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0025] Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0026] Furthermore, the dosage form of the pesticide composition is selected from solid preparations and / or liquid preparations and / or seed treatment preparations;

[0027] Furthermore, the solid preparations include powders, granules, balls, tablets, strips, wettable powders, oil-dispersed powders, milk powders, water-dispersible granules, milk granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0028] Furthermore, the liquid preparations include soluble solutions, soluble sols, oils, film-forming oils, emulsifiable concentrates, latexes, dispersible liquids, pastes, emulsions, oil emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspension emulsions, microcapsule suspension-suspensions, microcapsule suspension-emulsions or microcapsule suspension-suspension emulsions;

[0029] Furthermore, the pesticide composition can be prepared into a pharmaceutically acceptable dosage form, and the dosage form is a microemulsion, an emulsifiable concentrate, a suspension, an emulsion, a water-dispersible granule, a seed treatment suspension.

[0030] The present invention also discloses the use of a pesticide composition as described above in controlling plant diseases;

[0031] Furthermore, the plant diseases are plant diseases caused by fungi;

[0032] Furthermore, the fungi are Fusarium;

[0033] Further, the Fusarium genus includes Fusarium graminearum, Fusarium culmorum, Fusarium pseudograminearum, Fusarium oxysporum, Fusarium equiseti, Fusarium proliferatum, Fusarium chlamydosporum, Fusarium avenaceum, Fusarium solani, Fusarium asiaticum, Fusarium acuminatum, Fusarium moniliforme, Fusarium tricinctum, Fusarium poae, Fusarium lateritium, Fusarium verticillioides, Fusarium concolor;

[0034] Further, the Fusarium genus is Fusarium graminearum, Fusarium culmorum;

[0035] Further, the plant diseases caused by the Fusarium genus are wheat basal stalk rot, corn basal stalk rot, wheat head blight, wheat root rot;

[0036] The pathogens causing wheat basal stalk rot specifically include: Fusarium graminearum, Fusarium culmorum, Fusarium pseudograminearum, Fusarium oxysporum, Fusarium equiseti, Fusarium proliferatum, Fusarium chlamydosporum, Fusarium avenaceum, Fusarium solani, Fusarium asiaticum, Fusarium acuminatum; it is a soil-borne fungal disease caused by fungi of the genus Fusarium infecting the basal part of the wheat stalk, which can cause damage throughout the growth period. At the initial stage of the disease, it can lead to abnormal seed germination, root rot, and yellowing of leaves; as the disease progresses, in the middle and late stages, the basal part of the stalk turns brown and is easily broken, and red or white mold layers appear at the stem nodes, ultimately resulting in white ears in wheat plants, with underdeveloped grains or even no seeds, greatly affecting the wheat yield. In addition, after being infected by the pathogen, a series of bioactive secondary metabolites mainly composed of trichothecene mycotoxins will be produced in the plant. These toxins can inhibit the synthesis of cellular DNA, RNA, ribosomal proteins, and cellular proteins, activate the ribotoxic stress response, and ingestion of wheat containing such toxins will pose a serious threat to the life and health of humans and other mammals.

[0037] The pathogens causing maize basal stalk rot specifically include: Fusarium graminearum, Fusarium culmorum, Fusarium proliferatum, Fusarium solani, Fusarium moniliforme, Fusarium verticillioides, Fusarium concolor;

[0038] The pathogens of wheat scab specifically include: Fusarium graminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium equiseti, Fusarium avenaceum, Fusarium asiaticum, Fusarium acuminatum, Fusarium moniliforme, Fusarium tricinctum, Fusarium poae, Fusarium lateritium;

[0039] The pathogens of wheat root rot specifically include: Fusarium graminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium proliferatum, Fusarium avenaceum, Fusarium moniliforme. The pathogens of wheat root rot are complex, including Fusarium and Bipolaris sorokiniana. In addition to causing root rot, they also damage other parts of wheat plants, causing seedling rot, stem base rot, adult plant leaf blight and ear rot, etc., and there is some overlap in the symptoms caused by the pathogens of wheat stem base rot.

[0040] The beneficial effects of the present invention:

[0041] 1) The pesticide composition of the present invention has high control activity against Fusarium diseases, which is beneficial to realizing the effective prevention and control of diseases, and has a synergistic effect on the control effect at a certain mass ratio;

[0042] 2) The pesticide composition of the present invention can reduce the dosage of pesticides, is safe for crops, and has the effect of increasing production and ensuring harvest. Specific embodiments

[0043] To make the technical solutions, objectives and advantages of the present invention clearer and more understandable, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0044] Indoor toxicity determination:

[0045] Indoor bioassay of wheat scab

[0046] Test basis: NY / T 1156.2-2006 "Pesticide Bioassay Guidelines for Indoor Fungicides - Part 2: Inhibiting Mycelial Growth of Pathogenic Fungi - Petri Dish Method";

[0047] NY / T 1156.6-2006 "Pesticide Bioassay Guidelines for Indoor Fungicides - Part 6: Determination of Combined Action of Mixtures".

[0048] Tested pathogenic bacteria: Wheat basal rot;

[0049] Collect wheat plants with basal rot from wheat planting areas in various cities of Shandong Province. Cut the part with mildew layer at the junction of the basal stem nodes of the typical diseased plants, place it on the selective medium for cultivation. After the colonies grown show the typical characteristics of Fusarium colonies, transfer and preserve them. Select representative strains from them for single spore isolation, and identify the strains as Fusarium graminearum and Fusarium pseudograminearum through molecular identification respectively.

[0050] Tested pesticides: All tested pesticides are provided by the Group R & D Center.

[0051] Pesticide preparation: First dissolve the tested technical material with acetone, then dilute it with 0.1% Tween 80 aqueous solution to prepare single-agent mother liquors respectively, and set 5 series of mass concentrations according to the purpose of mixing and the activity of the pesticides.

[0052] Melt the PDA medium with a microwave oven, and cool it to about 50 °C. According to the principle from low concentration to high concentration, take 1 mL of the prepared test solution and 9 mL of PDA medium and add them into a Petri dish with a diameter of 9 cm, mix well to make the corresponding concentration of drug-containing plates.

[0053] Inoculation: For the cultivated pathogenic bacteria, punch out activated pathogenic fungi into fungal cakes with a puncher with a diameter of 6 mm under sterile conditions. After the drug-containing medium solidifies, place the fungal cakes at the center position of the medium. Finally, seal the Petri dish with sealing film, and then place it in an incubator at 28 °C and a relative humidity of 70% for cultivation. At the same time, set up a treatment without pesticides as a blank control, and repeat each treatment 3 times.

[0054] Investigation: According to the mycelial growth in the blank control Petri dish, investigate the mycelial growth of the pathogenic bacteria, and measure the colony diameter with a caliper, with the unit of millimeter (mm). Measure the diameter of each colony vertically once with the cross method, and take the average value.

[0055] Calculation method: According to the investigation results, calculate the mycelial growth inhibition rate according to the following formula, with the unit of percentage (%), and retain two decimal places for the calculation result.

[0056] D = D1 - D2

[0057] In the formula:

[0058] D —— Colony growth diameter;

[0059] D1 —— Colony diameter;

[0060] D2 —— Mycelial disc diameter.

[0061]

[0062] I —— Mycelial growth inhibition rate;

[0063] D0 —— Colony growth diameter of blank control;

[0064] D t —— Colony growth diameter treated with medicament.

[0065] Test statistics: The data was processed by the method of probit analysis. Analyzed with DPS statistical analysis system to obtain the toxicity regression line and EC 50 value, and evaluate the activity of the test medicament against the biological test materials.

[0066] Sun Yunpei method: Evaluate the synergistic effect of medicament mixture according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the mixture ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0067] The co-toxicity coefficient (CTC value) of the mixture is calculated by the following formula:

[0068]

[0069] In the formula:

[0070] ATI —— Measured toxicity index of the mixture;

[0071] S —— EC 50 , in milligrams per liter (mg / L);

[0072] M —— EC 50 , in milligrams per liter (mg / L).

[0073] TTI = TI A *P A +TI B *P B

[0074] In the formula:

[0075] TTI —— Theoretical toxicity index of the mixture;

[0076] TI A —— Toxicity index of medicament A;

[0077] P A —— The percentage content of Agent A in the mixture, in percentage (%)

[0078] TI B —— The virulence index of Agent B

[0079] P B —— The percentage content of Agent B in the mixture, in percentage (%)

[0080]

[0081] Wherein:

[0082] CTC - Co-toxicity coefficient

[0083] ATI - The measured virulence index of the mixture

[0084] TTI - The theoretical virulence index of the mixture

[0085] Test results:

[0086] Table 1 results show that in the range of the mixing mass ratio of Cyclobutrifluram to Bifemetstrobin from 1:40 to 20:1, the control effect on Fusarium graminearum shows additive or synergistic effects. Among them, when the mass ratio is from 1:25 to 20:1, the co-toxicity coefficient > 120, showing a synergistic effect. The co-toxicity coefficient value of Cyclobutrifluram and Bifemetstrobin = 1:2 is the largest, which is 198.801, and the EC 50 is 3.862 mg / L, with a significant synergistic effect

[0087] Table 1 Indoor determination results of different ratios on Fusarium graminearum

[0088]

[0089]

[0090] Table 2 results show that in the range of the mixing mass ratio of Cyclobutrifluram to Bifemetstrobin from 1:30 to 22:1, the control effect on Pseudograminearum shows a synergistic effect. Among them, when the mass ratio is from 1:18 to 14:1, the co-toxicity coefficient > 130, showing a synergistic effect. The co-toxicity coefficient value of Cyclobutrifluram and Bifemetstrobin = 2:1 is the largest, which is 236.497, and the EC 50 is 1.756 mg / L, with a significant synergistic effect

[0091] Table 2 Indoor determination results of different ratios against *Fusarium pseudograminearum*

[0092]

[0093] Formulation examples

[0094] Preparation Example 1: 45% Cyclobutrifluram·bifemetstrobin water dispersible granules (20:25)

[0095] Formulation: Cyclobutrifluram 20%, Bifemetstrobin 25%, sodium dodecyl sulfate 3%, naphthalene sulfonate formaldehyde condensate 8%, sodium lauryl ether sulfate 1%, sodium polycarboxylate 2%, sodium lignosulfonate 6.5%, attapulgite 5%, kaolin to make up the balance;

[0096] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional auxiliaries thereto, mix, add 10 - 25% of water after airflow pulverization, and then obtain the water dispersible granule product through kneading, granulation, drying, and screening; or spray water, granulate, and dry the pulverized powder in a fluidized bed granulator, and then screen to obtain the product.

[0097] Preparation Example 2: 18% Cyclobutrifluram·bifemetstrobin emulsifiable concentrate (8:10)

[0098] Formulation: Cyclobutrifluram 8%, Bifemetstrobin 10%, BHT 1.2%, propylene carbonate 10%, DMF 8%, dimethyl sulfoxide 5%, cyclohexanone 4%, calcium dodecylbenzenesulfonate 3%, fatty alcohol polyoxyethylene ether 12%, methyl oleate to make up the balance;

[0099] Preparation method: Add the active ingredients to the cosolvent according to the formula ratio of the example, and add surfactants and other functional auxiliaries thereto, and stir and mix evenly in a stirring and mixing kettle to obtain the emulsifiable concentrate.

[0100] Preparation Example 3: 7.5% Cyclobutrifluram·bifemetstrobin emulsion in water (2.5:5)

[0101] Formulation: Cyclobutrifluram 2.5%, Bifemetstrobin 5%, mesitylene 12%, cyclohexanone 8%, calcium dodecylbenzenesulfonate 1%, EO / PO block copolymer 4.5%, glycerol 5%, glycerin 1.5%, silicone defoamer 0.1%, xanthan gum 0.15%, sodium benzoate 0.1%, deionized water to make up the balance;

[0102] Preparation method: According to the formulation ratio of the examples, dissolve the active ingredients in a solvent and add an emulsifier to form a uniform oil phase. Mix deionized water, antifreeze, and additives to form a uniform aqueous phase. Under stirring, add the oil phase to the aqueous phase, stir evenly, shear to a qualified particle size, and then add additives such as defoaming agents and thickeners, and shear and stir evenly to form a well-dispersed water emulsion preparation.

[0103] Preparation Example 4: 30% Cyclobutrifluram·bifemetstrobin suspension concentrate (14:16)

[0104] Formulation of the preparation: Cyclobutrifluram 14%, Bifemetstrobin 16%, polyether 4%, naphthalene sulfonate formaldehyde condensate 3%, sodium dioctyl sulfosuccinate 3%, sodium polycarboxylate 1%, magnesium aluminum silicate 0.5%, xanthan gum 0.2%, ethylene glycol 5%, methylisothiazolinone 0.15%, silicone defoaming agent 0.5%, make up the balance with deionized water;

[0105] Preparation method: According to the formulation ratio, place the active ingredient, surfactant, and other functional additives in the reaction kettle in sequence, add water and mix evenly, and obtain the suspension concentrate through high-speed shearing, wet grinding, and finally homogenization and filtration.

[0106] Field efficacy test

[0107] Field efficacy test of wheat basal rot

[0108] Test basis: Refer to the industry standard "Pesticide Field Efficacy Test Guidelines Part 15: Fungicides for Controlling Wheat Head Blight (NY / T 1464.15 - 2007)";

[0109] Test crop: Wheat (Yannong 988);

[0110] Test location: Wheat field in Lugouya Village, Rizhao City, Shandong Province;

[0111] Test target: Collect wheat disease samples in the field before the test, and determine them as Fusarium pseudograminearum after molecular identification;

[0112] Test setup: Each plot is distributed according to a randomized block design, and a protection row is set around each plot. Each treatment has 4 replicates, and each plot is 20m 2 , and the plots are randomly arranged.

[0113] Application period: Apply the pesticide once during the greening stage of wheat, apply the pesticide once every 7 days, and apply the pesticide 2 times in total. Use the conventional spraying method, and evenly spray on both the front and back sides of the leaves. The spraying equipment is a knapsack electric sprayer, and the water consumption is 60L / mu.

[0114] Test drug:

[0115] Table 3 Experimental treatment

[0116] Trial Treatments Agent <![CDATA[Dosage g a.i / hm 2 > 1 7.5% Cyclobutrifluram·Bifemetstrobin Emulsion in Water (2.5:5) 60 2 30% Cyclobutrifluram·Bifemetstrobin Suspension Concentrate (14:16) 60 3 25% Cyclobutrifluram Suspension Concentrate 50 4 30% Bifemetstrobin Suspension Concentrate 200 5 Water Control /

[0117] Survey and statistical methods: Surveys were conducted once at the jointing stage (35 days after application of pesticides, visual observation) and once at the heading stage, for a total of two surveys. Sampling was carried out at 5 fixed points on the diagonal of each plot, and 60 plants / 100 to 200 ears were surveyed at each point. The incidence and disease rate of wheat were recorded in detail.

[0118] Grading method:

[0119] Level 0: There are no lesions on the leaf sheaths and stems;

[0120] Level 1: The leaf sheath is infected but the stem is not invaded;

[0121] Level 2: The lesions invade less than 1 / 4 of the stem circumference;

[0122] Level 3: The lesions invade less than 1 / 4 to 1 / 2 of the stem circumference;

[0123] Level 4: The lesions invade less than 1 / 2 to 3 / 4 of the stem circumference;

[0124] Level 5: The lesions invade less than 3 / 4 of the stem circumference, resulting in white ears or no ears due to the disease.

[0125] Calculation method of drug efficacy

[0126] The disease index and control efficacy were calculated using the following formula

[0127]

[0128] Direct impact on crops: Observe whether the pesticide has any damage to crops, and record the type and severity of the damage.

[0129] Effects on other organisms: Any evidence of effects on other pests and diseases should be recorded, including beneficial or unbeneficial effects; record the effects of the agent on other non-target organisms in the test area.

[0130] The test results are shown in the following table:

[0131] Table 4 Field efficacy test results of various treatment mixtures for controlling wheat stem rot

[0132]

[0133] Note: The control effect % in the above table is the average value of each replicate; lowercase letters represent significant differences at the 5% level.

[0134] During the experiment, wheat in each treatment plot grew well and no pesticide damage was observed in any treatment.

[0135] During the investigation at the full heading stage of wheat, as can be seen from the above Table 4 of the field efficacy test, the control effects of 7.5% Cyclobutrifluram·bifemetstrobin aqueous emulsion (2.5:5) and 30% Cyclobutrifluram·bifemetstrobin suspension concentrate (14:16) against wheat basal stalk rot were 82.44% and 82.52% respectively, both showing good control effects.

[0136] No phytotoxicity and no impact on crop growth and development were recorded during the test, indicating that the test agents are safe for crops within the recommended dosage. No impact on non-target organisms was found within the dosage range of the agents. No extreme weather conditions such as heavy rainfall and strong winds were recorded during the test.

[0137] Through indoor toxicity determination and field tests, the composition obtained by compounding Cyclobutrifluram and Bifemetstrobin described in the present invention shows very good control effects against wheat basal stalk rot. The control effect of the pesticide composition or its formulation obtained by compounding in the present invention is significant, with characteristics such as high efficiency, broad spectrum, low residue, long-lasting efficacy, and strong systemic property; and no phytotoxicity to crops was found in the test, indicating that under the condition of improved bactericidal synergistic effect of the obtained pesticide composition or formulation, the production cost and usage cost can be reduced, and it is safe for crops.

[0138] Although the present invention has been described in detail with general descriptions and specific embodiments above, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A pesticide composition, characterized in that: The invention comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is Cyclobutrifluram and the active ingredient B is Bifemetstrobin.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:40 to 22:1; Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 22:

1.

3. The pesticide composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:25 to 20:

1.

4. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1 to 90 wt %, preferably 5 to 80 wt %.

5. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also contains agriculturally permitted auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

6. The pesticide composition according to claim 5, characterized in that The dosage form of the pesticide composition is selected from solid preparations and / or liquid preparations.

7. The pesticide composition according to claim 6, characterized in that The pesticide composition can be prepared into a pesticide acceptable formulation, which is a microemulsion, emulsifiable concentrate, suspension, water emulsion, water dispersible granules, and seed treatment suspension.

8. Use of the pesticide composition according to any one of claims 1 to 7 in preventing and controlling plant diseases.

9. The use according to claim 8, characterized in that The plant disease is a plant disease caused by fungi; Preferably, the fungus is of the genus Fusarium.

10. The use according to claim 9, characterized in that The plant diseases caused by the genus Fusarium are wheat stem base rot, corn stem base rot, wheat fusarium rust and wheat root rot.

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