Functional feed additive and preparation method thereof

Abstract

The invention relates to the field of feed, and discloses a functional feed additive which is prepared from the following raw materials in parts by weight: 5-10 parts of carbonate compound, 15-65 parts of compound mineral element additive, 30-50 parts of reducing sugar, 2-10 parts of compound enzyme, 1-2 parts of bacillus subtilis CICC 208721 and 0.1-1 part of phagostimulant peptide. The functional feed additive is added into the basic ration, so that the active state of animals can be improved, the feed conversion rate can be effectively increased, the crude protein level in the feed can be effectively reduced, the feed cost is saved, and the economic benefit is increased.

Description

Technical Field

[0001] This application relates to the field of feeds, and particularly to a functional feed additive and its preparation method. Background Art

[0002] With the rapid development of the livestock industry, the breeding mode has gradually tended towards large-scale and intensive to meet the growing market demand. However, this breeding mode has also brought many challenges. Livestock and poultry are more vulnerable to diseases in the intensive breeding environment, resulting in a decline in productivity and high mortality. Traditionally, antibiotics and growth hormones have been widely used in feeds to improve the immunity of livestock and poultry and prevent diseases. However, the long-term use of antibiotics not only causes drug residues and drug resistance problems, but also weakens the natural immunity of livestock and poultry, thus affecting their health and product quality.

[0003] In recent years, with the improvement of consumers' awareness of food safety and environmental protection, the development of green and efficient feed additives to replace antibiotics has become the focus of industry research. At present, the research directions mainly focus on natural or biosynthetic active substances such as probiotics, enzyme preparations, and plant extracts. These additives can not only improve the health status and production performance of livestock and poultry, but also reduce environmental pollution.

[0004] In addition, the reasonable addition of trace elements in feeds is also crucial for the health of livestock and poultry. The complex soil types result in significant differences in the content of trace elements in feed raw materials. The common practice is to add without considering the content of the basal diet and exceeding (1-3 times the NRC recommendation), resulting in excessive and unbalanced trace element content in the diet, affecting animal production performance and health. Most elements are discharged into the environment with livestock and poultry manure, leading to serious environmental pollution.

[0005] The present invention aims to develop a new type of functional feed additive, accurately and efficiently add various raw materials, provide a solution that can not only enhance the immunity of livestock and poultry, promote growth, but also reduce drug residues and environmental pollution, and promote the sustainable development of the livestock industry. Summary of the Invention

[0006] In view of the above problems existing in the prior art, the present invention provides a functional feed additive and its preparation method.

[0007] In a first aspect, the present application provides a functional feed additive, which is composed of the following raw materials in parts by weight: 5-10 parts of carbonate compound, 15-65 parts of compound mineral element additive, 30-50 parts of reducing sugar, 2-10 parts of compound enzyme, 1-2 parts of Bacillus subtilis CICC 20872, and 0.1-1 part of feeding attractant peptide.

[0008] In an embodiment of the present application, the carbonate compound is selected from at least one of sodium bicarbonate, potassium carbonate, and sodium carbonate.

[0009] The carbonate compound is a potassium or sodium compound. Potassium can maintain the ion balance in animals, especially the water balance, osmotic pressure, and acid-base balance in the body. Moreover, potassium is an activator of various enzymes, including synthetases, oxidoreductases, and transferases, and participates in the anabolic metabolism of sugars and proteins within cells.

[0010] Sodium ions and potassium ions play important roles in aspects such as the electrophysiological properties, permeability, excitability, and osmotic pressure balance of cell membranes by maintaining the ion concentration gradient and potential difference inside and outside cells, and are the basis for normal cell functions.

[0011] In one embodiment of the present application, the composite mineral element additive includes a silicon element additive, a potassium element additive, a boron element additive, a titanium element additive, a nickel element additive, a vanadium element additive, a selenium element additive, a molybdenum element additive, a chromium element additive, a lanthanum element additive, and a cerium element additive.

[0012] In one embodiment of the present application, the silicon element additive is selected from crystalline hydrated aluminosilicates.

[0013] In one embodiment of the present application, the potassium element additive is selected from potassium dihydrogen phosphate.

[0014] In one embodiment of the present application, the boron element additive is selected from sodium borate.

[0015] In one embodiment of the present application, the titanium element additive is selected from citric acid chelated titanium.

[0016] In one embodiment of the present application, the nickel element additive is selected from organic nickel compounds.

[0017] In one embodiment of the present application, the organic nickel compound is selected from nickel methionine.

[0018] In one embodiment of the present application, the vanadium element additive is selected from ammonium metavanadate.

[0019] In one embodiment of the present application, the selenium element additive is selected from at least one of yeast selenium and sodium selenite.

[0020] In one embodiment of the present application, the molybdenum element additive is selected from sodium molybdate.

[0021] In one embodiment of the present application, the chromium element additive is selected from chromium methionine.

[0022] In one embodiment of the present application, the lanthanum element additive is selected from rare earth lanthanum amino acids.

[0023] In one embodiment of the present application, the cerium element additive is selected from rare earth cerium amino acids.

[0024] In one embodiment of the present application, the amino acid is selected from at least one of glycine, alanine, aspartic acid, glutamic acid, proline, tyrosine, tryptophan, and lysine.

[0025] In one embodiment of the present application, the crystalline hydrated aluminosilicate can be used as a carrier to encapsulate other mineral elements or trace elements inside to prevent their loss or oxidation in the feed; the crystalline hydrated aluminosilicate can effectively reduce the content of anti-nutritional factors in the feed and regulate the intestinal biological flora. Silicon promotes bone growth and development and has a physiological role in the process of bone calcification; it participates in polysaccharide metabolism and is a main component of some aminoglucan protein complexes. Boron participates in the synthesis of coenzyme A and coenzyme Q, plays an important role in the tricarboxylic acid cycle and electron transfer process, can promote the metabolism of mineral elements such as calcium, phosphorus, magnesium, copper, iron, manganese, zinc, vitamin D3, amino acids, proteins, and fats, and boron is a necessary material for the formation of ribonucleic acid.

[0026] Titanium participates in the biological oxidation process of the body, can increase the coupling effect of oxidative acyl groups, and enhance the mitochondrial oxidative phosphorylation of the liver and heart.

[0027] Nickel participates in biological reactions as a structural component or activating factor of enzymes, stimulates hematopoiesis function, regulates the metabolism of nucleic acids and proteins, and can also be used as an auxiliary factor for biological ligands.

[0028] The chemical structure of organic nickel compounds has higher stability in the animal body, with higher bioavailability, less irritation to the gastrointestinal tract, and reduced nickel emissions in animal excrement.

[0029] Nickel methionine is absorbed and metabolized gently in the animal body, with fewer side effects in animals.

[0030] Selenium participates in the composition of glutathione peroxidase, can reduce toxic peroxides to harmless substances, can decompose hydrogen peroxide, has a protective effect on cells and tissues, protects them from damage by peroxides, has a special protective effect on cell membranes and organelle membranes (such as mitochondria, lysosomes, etc.), and enhances the body's immunity. Molybdenum is mainly used as a component of xanthine oxidase or dehydrogenase and aldehyde oxidase, participates in the redox reaction in the body, and can eliminate the harm of free radicals.

[0031] Chromium forms an organic chelate with niacin, glycine, and cystine, which plays an important role in regulating the metabolism of carbohydrates, fats, and proteins, and helps with metabolism in the animal body and resists the influence of stress.

[0032] Rare earth lanthanum amino acid and rare earth cerium amino acid can promote growth, improve immunity, and improve feed utilization rate in animal breeding.

[0033] In one embodiment of the present application, rare earth lanthanum amino acid and rare earth cerium amino acid can be purchased commercially.

[0034] In one embodiment of the present application, the complex enzyme includes xylanase, acidic mannanase, β-glucanase, cellulase, α-galactosidase and β-mannanase.

[0035] Xylanase can improve feed performance and eliminate or reduce the anti-nutritional effects caused by increased viscosity.

[0036] Acidic mannanase can effectively reduce its anti-nutritional effects, improve nutrient digestibility, and improve animal production performance.

[0037] β-glucanase can effectively improve the digestion and absorption of nutrients by monogastric animals, improve production performance, and improve feed conversion rate.

[0038] Cellulase can supplement the deficiency of animal endogenous enzymes, improve the digestion and absorption rate of nutrients, remove feed anti-nutritional factors, and improve the relationship of the flora in the digestive tract.

[0039] α-galactosidase can improve the utilization efficiency of nutrients in feed, greatly reduce diarrhea, and enhance the immune function and disease resistance of animals.

[0040] β-mannanase can eliminate the interference of mannan in feed on glucose absorption, improve the flora composition, reduce intestinal viscosity, and promote the digestion and absorption of energy, protein and cellulose.

[0041] In one embodiment of the present application, the functional feed additive is composed of the following raw materials in parts by weight: 5-10 parts of carbonate compound, 5-24 parts of crystalline hydrated silicoaluminate, 1-2 parts of potassium dihydrogen phosphate, 10-20 parts of sodium borate, 0.5-2 parts of titanium citrate chelate, 0.007-0.04 parts of nickel methionine, 0.001-0.005 parts of ammonium metavanadate, 0.8-1.5 parts of yeast selenium, 0.3-1.2 parts of sodium selenite, 0.01-0.1 parts of sodium molybdate, 0.001-0.005 parts of chromium methionine, 0.015-1.5 parts of rare earth lanthanum amino acid, 0.015-1.5 parts of rare earth cerium amino acid, 30-50 parts of reducing sugar, 2-10 parts of complex enzyme, 2-1 parts of Bacillus subtilis CICC 20872 and 0.1-1 part of feeding peptide.

[0042] In one embodiment of the present application, the reducing sugar is selected from at least one of glucose, fructose, sucrose and maltose.

[0043] Reducing sugar participates in cell signal transduction and maintains cell homeostasis by providing energy.

[0044] Both Bacillus subtilis CICC 20872 and the feeding attractant peptide can be obtained commercially.

[0045] The preparation used in this application is a powder preparation of Bacillus subtilis.

[0046] The feeding attractant peptide can improve the flavor of the feed additive, enhance its palatability, and increase its feed intake.

[0047] In a second aspect, the present application provides a method for preparing a functional feed additive, comprising the following steps: dissolving crystalline hydrated silicoaluminate, potassium dihydrogen phosphate, sodium borate, citric acid chelated titanium, nickel methionine, ammonium metavanadate, sodium selenite, sodium molybdate, chromium methionine, rare earth lanthanum amino acid, rare earth cerium amino acid, and reducing sugar in water, and carrying out a chelation reaction at 60 - 90 °C under the protection of a nitrogen gas stream while maintaining the redox potential at 20 - 65 mV to form a stable colloidal liquid, which is then spray-dried to obtain a powder. The powder is mixed uniformly with yeast selenium, complex enzyme, Bacillus subtilis CICC 20872, and the feeding attractant peptide to prepare the functional feed additive.

[0048] In one embodiment of the present application, through the chelation reaction, a stable colloidal liquid is formed, enabling each mineral element to be in an ionic state, and then dried into a powder as needed.

[0049] In one embodiment of the present application, the weight - volume ratio of the raw materials, namely crystalline hydrated silicoaluminate, potassium dihydrogen phosphate, sodium borate, citric acid chelated titanium, nickel methionine, ammonium metavanadate, sodium selenite, sodium molybdate, chromium methionine, rare earth lanthanum amino acid, rare earth cerium amino acid, and reducing sugar, to water is 0.6 - 0.8:1.

[0050] In one embodiment of the present application, the chelation reaction time is 3 - 5 h.

[0051] Preferably, the chelation reaction is carried out at 65 - 80 °C under the protection of a nitrogen gas stream while maintaining the redox potential at 20 - 65 mV.

[0052] In one embodiment of the present application, the content of the complex enzyme in the functional feed additive is 1 billion units / kg - 10 billion units / kg.

[0053] In one embodiment of the present application, the content of Bacillus subtilis CICC 20872 in the functional feed additive is 100 billion units / kg - 50 trillion units / kg.

[0054] In one embodiment of the present application, the content of the feeding attractant peptide in the functional feed additive is 1 billion units / kg - 10 billion units / kg.

[0055] In an embodiment of the present application, the content of molybdenum element in the functional feed additive is ≥ 20 - 3000 μg / kg.

[0056] In an embodiment of the present application, the content of molybdenum element in the functional feed additive is ≤ 2.5 mg / kg.

[0057] In an embodiment of the present application, the content of selenium element in the functional feed additive is ≥ 90 - 5000 μg / kg.

[0058] In an embodiment of the present application, the content of selenium element in the functional feed additive is < 0.1 - 0.5 mg / kg.

[0059] In an embodiment of the present application, the content of vanadium element in the functional feed additive is ≤ 10 mg / kg.

[0060] In a third aspect, the present application provides a compound feed including a functional feed additive.

[0061] In an embodiment of the present application, the functional feed additive accounts for 0.05 - 1 wt.% of the compound feed.

[0062] Compared with the prior art, the present invention has the following beneficial effects:

[0063] 1) By adding the functional feed additive to the basal diet in the present application, the absorption, transformation and metabolism level of animals can be significantly improved, the feed conversion rate can be effectively increased, and the breeding benefit can be increased.

[0064] 2) By adding the functional feed additive to the basal diet in the present application, the active state of the chicken flock can be improved, and the color and state of the chicken combs can be improved; the quality of eggshells can be enhanced, the number of cracked and flowered eggs can be reduced, and the uniformity can be increased; the intestinal health of chickens can be regulated, the activity of the chicken flock can be increased, and the function of the isthmus of chickens can be improved.

[0065] 3) The functional feed additive of the present application can effectively reduce the crude protein level in the feed, save the feed cost, and thus increase the economic benefit.

[0066] 4) The functional feed additive has a tendency to increase the milk yield, and at the same time can increase the milk fat rate and lactose content, and significantly reduce the somatic cell count in the milk, improving the quality of milk. BRIEF DESCRIPTION OF THE DRAWINGS

[0067] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art.

[0068] Figure 1 It is a diagram of the state of the chicken flock on the first day of the experimental group;

[0069] Figure 2 It is a diagram of the flock status on the fourteenth day of the experimental group;

[0070] Figure 3 It is a diagram of the eggshell color on the first day of the experimental group;

[0071] Figure 4 It is a diagram of the eggshell color on the fourteenth day of the experimental group. Specific implementation manners

[0072] The technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present application.

[0073] The "range" disclosed in the present application is defined in the form of a lower limit and an upper limit. A given range is defined by selecting a lower limit and an upper limit, and the selected lower limit and upper limit define the boundary of the specific range. The range defined in this way can include the end values or not include the end values, and can be combined arbitrarily, that is, any lower limit can be combined with any upper limit to form a range.

[0074] Example 1

[0075] Dissolve 2.5 parts of sodium bicarbonate, 2.5 parts of potassium carbonate, 2.5 parts of sodium carbonate, 14.5 parts of crystalline hydrated silicoaluminate, 1.5 parts of potassium dihydrogen phosphate, 15 parts of sodium borate, 1.25 parts of citric acid chelated titanium, 0.025 parts of nickel methionine, 0.025 parts of ammonium metavanadate, 0.75 parts of sodium selenite, 0.05 parts of sodium molybdate, 0.003 parts of chromium methionine, 0.75 parts of rare earth lanthanum amino acid, 0.75 parts of rare earth cerium amino acid and 40 parts of reducing sugar in water according to the weight-to-volume ratio of 0.7:1. Carry out a chelation reaction at 75°C under the protection of a nitrogen gas stream, maintaining the redox potential at 40 mV for 4 hours to form a stable colloidal liquid. Obtain a powder by spray drying. Mix the powder evenly with 1.15 parts of yeast selenium, 6 parts of complex enzyme (1 part each of xylanase, acidic mannanase, β-glucanase, cellulase, α-galactosidase and β-mannanase), 1.5 parts of Bacillus subtilis CICC 20872 and 0.5 parts of feeding attractant peptide to prepare a functional feed additive. Take samples for testing to ensure that the content of the complex enzyme in the functional feed additive is within 1 billion units / kg - 10 billion units / kg, the content of Bacillus subtilis CICC 20872 is within 100 billion units / kg - 50 trillion units / kg, the content of the feeding attractant peptide is within 1 billion units / kg - 10 billion units / kg, the content of molybdenum element is ≥20 - 3000 μg / kg and the content of molybdenum element is ≤2.5 mg / kg, and the content of selenium element is ≥90 - 5000 μg / kg and the content of selenium element is <0.1 - 0.5 mg / kg.

[0076] Example 2

[0077] Dissolve 3 parts of sodium bicarbonate, 3 parts of potassium carbonate, 3 parts of sodium carbonate, 20 parts of crystalline hydrated silicoaluminate, 2 parts of potassium dihydrogen phosphate, 20 parts of sodium borate, 2 parts of citric acid chelated titanium, 0.04 part of nickel methionine, 0.005 part of ammonium metavanadate, 1.2 parts of sodium selenite, 0.1 part of sodium molybdate, 0.005 part of chromium methionine, 1.5 parts of rare earth lanthanum amino acid, 1.5 parts of rare earth cerium amino acid and 50 parts of reducing sugar in water according to the weight - volume ratio of 0.8:1. At 90 °C, under the protection of nitrogen gas flow, keep the redox potential at 60 mv and carry out chelation reaction for 5 h to form a stable colloidal liquid. After spray - drying, obtain a powder. Mix the powder evenly with 1.5 parts of yeast selenium, 9 parts of compound enzyme (1.5 parts each of xylanase, acidic mannanase, β - glucanase, cellulase, α - galactosidase and β - mannanase), 22 parts of Bacillus subtilis CICC 20872 and 1 part of feeding attractant peptide to prepare a functional feed additive. Take samples for detection to ensure that the content of the compound enzyme in the functional feed additive is within 1 billion units / kg - 10 billion units / kg, the content of Bacillus subtilis CICC 20872 is within 100 billion units / kg - 50 trillion units / kg, the content of the feeding attractant peptide is within 1 billion units / kg - 10 billion units / kg, the content of molybdenum element is ≥20 - 3000 μg / kg and the content of molybdenum element is ≤2.5 mg / kg, and the content of selenium element is ≥90 - 5000 μg / kg and the content of selenium element is <0.1 - 0.5 mg / kg.

[0078] Example 3

[0079] Dissolve 1.5 parts of sodium bicarbonate, 1.5 parts of potassium carbonate, 1.5 parts of sodium carbonate, 5 parts of crystalline hydrated silicoaluminate, 1 part of potassium dihydrogen phosphate, 10 parts of sodium borate, 0.5 part of titanium citrate chelate, 0.01 part of nickel methionine, 0.001 part of ammonium metavanadate, 0.3 part of sodium selenite, 0.01 part of sodium molybdate, 0.001 part of chromium methionine, 0.015 part of rare earth lanthanum amino acid, 0.015 part of rare earth cerium amino acid, and 30 parts of reducing sugar in water according to a weight-to-volume ratio of 0.6:1. Conduct a chelation reaction at 60°C under a nitrogen gas flow protection, maintaining the redox potential at 20 mV for 3 hours to form a stable colloidal liquid. Obtain a powder through spray drying. Mix the powder evenly with 1.0 part of yeast selenium, 3 parts of compound enzyme (0.5 part each of xylanase, acidic mannanase, β-glucanase, cellulase, α-galactosidase, and β-mannanase), 21 parts of Bacillus subtilis CICC 20872, and 0.1 part of feeding attractant peptide to prepare a functional feed additive. Take samples for testing to ensure that the content of the compound enzyme in the functional feed additive is within 1 billion units / kg - 10 billion units / kg, the content of Bacillus subtilis CICC 20872 is within 100 billion units / kg - 50 trillion units / kg, the content of the feeding attractant peptide is within 1 billion units / kg - 10 billion units / kg, the content of molybdenum element is ≥20 - 3000 μg / kg and the content of molybdenum element is ≤2.5 mg / kg, and the content of selenium element is ≥90 - 5000 μg / kg and the content of selenium element is <0.1 - 0.5 mg / kg.

[0080] Example 4

[0081] (1) Test materials

[0082] Test time: August 1, 2024 - August 23, 2024 (23 days);

[0083] Laying hen breed and age: Hy-Line Gray laying hens, 200 days old;

[0084] Test group: Add 10 kg of the functional feed additive prepared in Example 1 to every 1 ton of the basal diet. The basal diet variety selected in this example is Anyou feed.

[0085] Control group: Select the basal diet as the control group. The basal diet variety selected in this example is Anyou feed.

[0086] (2) Test methods and results

[0087] The chickens in the experimental group and the control group are both Hy-Line Gray laying hens at 200 days old of the same batch. At the beginning, there were 43,532 chickens in the experimental group and 43,812 chickens in the control group. The experimental group was fed a compound feed supplemented with a functional feed additive; the control group was fed a basal diet without the addition of a functional feed additive, and it was added throughout the experiment until the end. During the experiment, the daily inventory, daily feed intake, egg weight, daily egg production, egg production rate, number of cracked and broken eggs, daily death and culling number, feed-to-egg ratio, egg color, fecal condition, and medication condition of the experimental group and the control group were recorded every day.

[0088] As can be seen from Table 1, from the average value in the week before the experiment started, the average egg weight of the control group and the experimental group differed by 0.2 g / egg. In the first week after use, the egg weight of the experimental group was 0.6 g / egg heavier than that of the control group. In the second week after use, it was 0.8 g / egg heavier than the control group, and in the third week, it was 0.8 g heavier than the control group. During the entire experimental period, the average egg weight of the experimental group was 0.7 g / egg heavier than that of the control group. The egg production rate of the experimental group was 1.39 percentage points higher than that of the control group in the week before use. In the first week after use, the egg production rate of the experimental group was 1.88 percentage points higher than that of the control group. In the second week after use, it was 2.71 percentage points higher than the control group, and in the third week, it was 2.83 percentage points higher than the control group. During the entire experimental period, the average egg production rate of the experimental group was 2.48 percentage points higher than that of the control group. The number of cracked and broken eggs in the experimental group and the control group was not much different in the week before, and it was 18 eggs / day lower than that of the control group. In the first week after use, the experimental group had 205 eggs / day fewer than the control group, 144 eggs / day fewer in the second week, and 128 eggs fewer in the third week. During the entire experimental period, the average number of cracked and broken eggs in the experimental group was 159 eggs / day fewer than that of the control group.

[0089] The daily death and culling number of the experimental group was 3.2 chickens / day fewer than that of the control group in the week before use. In the first week after use, the difference between the two groups was not significant. In the second week after use, the experimental group had 4.4 chickens / day fewer than the control group, and in the third week, it had 2 chickens / day fewer than the control group. During the entire experimental period, the average death and culling number of the experimental group was 2.1 chickens / day fewer than that of the control group. The average daily feed intake of the experimental group and the control group was not much different during the experiment. The experimental group had 1,531 more eggs per day on average than the control group. The feed-to-egg ratio of the experimental group was 0.07 lower than that of the control group. The egg weight of the experimental group was 164.6 catties heavier than that of the control group on average per day.

[0090] As can be seen from Table 2, the state of the chicken flock in the control group was rather dull, the chicken combs were completely collapsed, and the color of the chicken combs was relatively light. Two weeks after use, the chicken flock in the experimental group was lively, the chicken combs were completely erected, and the color was also relatively bright red. There were relatively more cracked, broken, and small eggs in the control group. Two weeks after use, there were fewer cracked, broken, and small eggs in the experimental group, and the color uniformity was good and it was relatively smooth. The feces of the control group were initially soft, not in lumps, and some were dry and hard. After use, the feces of the chickens in the experimental group were formed, moist, and relatively healthy.

[0091] Table 1

[0092]

[0093] Table 2

[0094]

[0095] It can be seen that adding functional feed additives to the basal diet can significantly increase the egg production rate by 2.48 percentage points; it can increase the egg weight by 0.7 g / egg, reduce the number of cracked and spoiled eggs by 159 per day, reduce the daily death and culling rate by nearly 50%, and reduce the feed-to-egg ratio by 0.07. This shows that directly adding functional feed additives can significantly improve the absorption, transformation and metabolism level of laying hens, effectively improve the feed conversion rate, and increase the breeding efficiency.

[0096] Adding functional feed additives to the basal diet can significantly improve the activity state of the chicken flock and the color and state of the chicken combs; it can improve the quality of eggshells, reduce the number of cracked, spoiled and small eggs, and increase the uniformity; it can also improve the feces, making the feces formed and moist. This shows that RP beneficial protein can regulate the intestinal health of chickens, increase the activity of the chicken flock, and improve the function of the isthmus of chickens.

[0097] Example 5

[0098] (1) Test materials

[0099] Test time: 87 days;

[0100] Pig breed: Duroc × (Landrace × Yorkshire) ternary pigs;

[0101] Test equipment: Osborne pig performance measurement instrument;

[0102] Test group: On the basis of the control group, reduce the crude protein by 1% and add 0.5 kg of the functional feed additive prepared in Example 1 per ton;

[0103] Control group: Select the basal diet as the control group, and the basal diet is formulated by Zhejiang Hanbei Biotechnology Co., Ltd.

[0104] (2) Test methods and results

[0105] Thirty-six nursery pigs with similar breeds, weights and half males and half females were randomly divided into two groups, with 12 pigs in each group, namely the test group and the control group. Each pig in each group was regarded as a replicate.

[0106] Before the experiment, an electronic ear tag was attached to each pig, and each instrument was calibrated. Each instrument could only allow one pig to eat at a time, and the electronic ear number, feed intake, and body weight of the pig were automatically transmitted to the instrument. Data needed to be imported once a day (importing the data in the instrument into the computer). The blind experiment method was adopted, that is, it was not clear whether the added feed was the control feed or the test feed. Only the feed added to each group needed to be the same each time. The purpose was to make the experiment more persuasive and avoid some unnecessary reasons caused by humans leading to directional results. The type of feed would be notified after the experiment ended.

[0107] During the experiment, the drinking water, vaccine immunization, and group medication of the two groups needed to be the same. Observe the health status of the pig group every day, and record the feed intake and body weight of each pig. Calculate the feed-to-gain ratio on a weekly basis until the pigs were sold. The results are shown in Table 3. Among them, the recorded days were valid days, and the system automatically excluded the days with large data errors or no data; the average daily feed intake ADFI = total feed intake / recorded days; the average daily gain ADG was the regression line slope value of the body weight increment within the selected measurement date; the feed conversion ratio = average daily feed intake / average daily gain.

[0108] Table 3

[0109]

[0110] It can be seen from Table 3 that after adding the functional feed additive and reducing the crude protein in the feed by 1%, it not only does not affect the growth of fattening pigs, but can also increase the growth rate of fattening pigs. It fully shows that adding the functional feed additive to the feed can improve the digestion and absorption of nutrients in the feed by fattening pigs, effectively reduce the crude protein level in the feed, thereby saving feed costs and increasing economic benefits. Observe the overall state of the pig group. The hair color and body shape of the control group are normal, and the hair color of the experimental group is smoother and the body shape is wider.

[0111] Example 6

[0112] (1) Experimental materials

[0113] Experimental time: 90 days;

[0114] Cows: Select cows with the same age, lactation stage, and breed;

[0115] Experimental group: 10 kg of the functional feed additive prepared in Example 1 was mixed with every 1 ton of concentrate feed.

[0116] Control group: Concentrate feed.

[0117] (2) Experimental methods and results

[0118] Select 100 cows with the same age, breed, and milk production time, and randomly divide them into two groups, with 50 cows in each group.

[0119] The experimental group and the control group should be consistent in terms of feeding methods, management levels, milking times, medications, etc.

[0120] During the experiment, the milk production of each group should be recorded every day. The storage conditions of the milk in each group should be the same.

[0121] When the milk of each group is sent to the dairy factory, the test data after inspection of each batch should be requested, including indicators such as somatic cells, milk production, milk fat rate, lactose, etc. The average value of each indicator for 30 days should be used as one set of data.

[0122] After the experiment, each result should be statistically analyzed.

[0123] As can be seen from Table 4, the number of somatic cells in the experimental group has always been less than that in the control group, indicating that adding functional feed additives has the tendency to reduce the number of somatic cells in cow milk.

[0124] Table 4 Somatic cell count in milk of each group (unit: 10,000 / mL)

[0125]

[0126] As can be seen from Table 5, during the entire experimental period, the milk production of the experimental group was higher than that of the control group. It was 0.73, 1.03, 1.41, and 1.13 kg more respectively in 4 time periods. The milk production of the experimental group increased by 3.17%, 4.51%, 6.10%, and 4.95% respectively compared with the control group. Therefore, it can be concluded that functional feed additives can increase the milk production of cows.

[0127] Table 5 Milk production of each group (unit: kg)

[0128]

[0129] As can be seen from Table 6, at the beginning of the experiment, the milk fat rate of the experimental group was lower than that of the control group. However, after using functional feed additives, the milk fat rate of the experimental group was higher than that of the control group, being 4.68%, 1.79%, and 3.79% higher respectively, indicating that functional feed additives can increase the milk fat rate in milk.

[0130] Table 6 Data of milk fat rate in milk of each group (unit: %)

[0131]

[0132] As can be seen from Table 7, the experimental group has a tendency to increase the lactose content in cows' milk, being 3.12%, 0.89%, and 3.09% higher than the control group at 30 days, 60 days, and 90 days respectively.

[0133] Functional feed additives have the tendency to increase milk production, can increase the milk fat rate and lactose content at the same time, and can significantly reduce the number of somatic cells in milk, improving the quality of milk.

[0134] The present application has been described in detail in conjunction with specific embodiments and exemplary examples. However, these descriptions should not be construed as limiting the present application. Those skilled in the art understand that, without departing from the spirit and scope of the present application, various equivalent substitutions, modifications, or improvements can be made to the technical solutions and implementation manners of the present application, and all of these fall within the scope of the present application. The protection scope of the present application is subject to the appended claims.

Claims

1. A functional feed additive, characterized in that: The invention is composed of the following raw materials in parts by weight: 5-10 parts of carbonate compounds, 15-65 parts of composite mineral element additives, 30-50 parts of reducing sugars, 2-10 parts of composite enzymes, 1-2 parts of Bacillus subtilis CICC20872 and 0.1-1 parts of attractant peptides.

2. The functional feed additive according to claim 1, characterized in that The carbonate compound is selected from at least one of sodium bicarbonate, potassium carbonate and sodium carbonate.

3. The functional feed additive according to claim 1, characterized in that The composite mineral element additives include silicon element additives, potassium element additives, boron element additives, titanium element additives, germanium element additives, nickel element additives, vanadium element additives, selenium element additives, molybdenum element additives, chromium element additives, lanthanum element additives and cerium element additives.

4. The functional feed additive according to claim 3, characterized in that The silicon element additive is selected from crystalline hydrated aluminosilicate; the potassium element additive is selected from potassium dihydrogen phosphate; the boron element additive is selected from sodium borate; the titanium element additive is selected from citric acid chelated titanium; the nickel element additive is selected from organic nickel compounds; the vanadium element additive is selected from ammonium metavanadate; the selenium element additive is selected from at least one of yeast selenium and sodium selenite; the molybdenum element additive is selected from sodium molybdate; the chromium element additive is selected from methionine chromium; the lanthanum element additive is selected from amino acid rare earth lanthanum; and the cerium element additive is selected from amino acid rare earth cerium.

5. The functional feed additive according to claim 1, characterized in that The complex enzyme comprises xylanase, acid mannanase, β-glucanase, cellulase, α-galactosidase and β-mannanase.

6. The functional feed additive according to claim 1, characterized in that: The invention is composed of the following raw materials in parts by weight: 5-10 parts of carbonate compounds, 5-24 parts of crystalline hydrated aluminosilicate, 1-2 parts of potassium dihydrogen phosphate, 10-20 parts of sodium borate, 0.5-2 parts of citric acid chelated titanium, 0.007-0.04 parts of organic nickel compounds, 0.001-0.005 parts of ammonium metavanadate, 0.8-1.5 parts of yeast selenium, 0.3-1.2 parts of sodium selenite, 0.01-0.1 parts of sodium molybdate, 0.001-0.005 parts of methionine chromium, 0.015-1.5 parts of amino acid rare earth lanthanum, 0.015-1.5 parts of amino acid rare earth cerium, 30-50 parts of reducing sugar, 2-10 parts of complex enzyme, 1-2 parts of Bacillus subtilis CICC 20872 and 0.1-1 parts of attractant peptide.

7. The functional feed additive according to claim 6, characterized in that The amino acid is selected from at least one of glycine, alanine, aspartic acid, glutamic acid, proline, tyrosine, tryptophan and lysine.

8. The method for preparing the functional feed additive according to any one of claims 1 to 7, characterized in that: The following steps are involved: Crystalline hydrated aluminosilicate, potassium dihydrogen phosphate, sodium borate, citric acid chelated titanium, organic nickel compound, ammonium metavanadate, sodium selenite, sodium molybdate, methionine chromium, amino acid rare earth lanthanum, amino acid rare earth cerium and reducing sugar are dissolved in water, and a chelation reaction is carried out at 60-90°C under the protection of nitrogen flow while maintaining a redox potential of 20-65mv to form a stable colloidal liquid, which is then spray-dried to obtain a powder, and the powder is evenly mixed with yeast selenium, complex enzyme, Bacillus subtilis CICC 20872 and attractant peptide to prepare a functional feed additive.

9. A compound feed, characterized in that: The functional feed additive comprises the functional feed additive according to any one of claims 1 to 5.

10. The compound feed according to claim 9, characterized in that The functional feed additive accounts for 0.05-1wt.% of the compound feed.

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