Plant anti-dandruff composition with scalp barrier repairing function as well as preparation and application of plant anti-dandruff composition

Abstract

The invention belongs to the field of daily cosmetics, and particularly relates to a plant anti-dandruff composition with a scalp barrier repairing function as well as preparation and application of the plant anti-dandruff composition. The plant anti-dandruff formula comprises the following components: a sebum inhibitor, a surfactant, fish oil glycerol mixed esters, hydrogenated polydecene and caprylic / capric glycerides, the sebum inhibitor comprises a sophora flavescens extract, a cynanchum atratum extract and a centella asiatica root extract; the preparation method of the cynanchum atratum extract comprises the following steps: adding a mixed solvent of lecithin, tripolyglycerol oleate and water into cynanchum atratum, soaking, extracting and concentrating to obtain the cynanchum atratum extract. According to the anti-dandruff formula provided by the invention, through careful selection and scientific proportioning, the components can effectively cooperate with one another, so that the inhibition effect on malassezia is remarkably improved, excellent anti-dandruff and itching-relieving effects are achieved, and the anti-dandruff formula has a good application prospect.

Description

Technical Field

[0001] The present invention belongs to the field of daily-use cosmetics, and particularly relates to a plant anti-dandruff formula with a scalp barrier repair function, its preparation and application. Background Art

[0002] In recent years, scalp health problems have become one of the hot topics focused on by the cosmetics industry. Traditional anti-dandruff products generally rely on chemically synthesized ingredients such as zinc pyrithione and ketoconazole. Although they can inhibit the reproduction of Malassezia in the short term, long-term use is likely to disrupt the scalp's microecological balance, thereby causing a series of problems such as damage to the barrier function and increased sensitivity. At the same time, consumers' growing preference for natural plant ingredients has promoted the market to develop towards the dual standards of "efficacy and mildness".

[0003] The limitations currently faced by plant anti-dandruff technologies include: most plant formulas are single, only focusing on anti-fungal or anti-dandruff effects, while ignoring the repair needs of the scalp barrier. For example, ingredients such as Platycladus orientalis leaf and Sophora flavescens root can regulate sebum secretion, but have no direct effect on the reconstruction of the lipid membrane; in addition, the transdermal absorption efficiency of plant extracts is relatively low. Due to their large molecular weight and insufficient lipophilicity, it is difficult to penetrate the stratum corneum and act on the deep layer of hair follicles.

[0004] In response to these limitations, modern research has gradually shifted towards the refined utilization of plant active ingredients. For example, bisabolol can inhibit the release of inflammatory factors, thereby repairing damaged epidermis; asiaticoside can promote the synthesis of collagen to strengthen the barrier structure. New pyrimidine derivatives such as minoxidil inhibit type II 5α-reductase, reduce DHT-induced follicular fibrosis, and indirectly optimize the scalp environment. However, there has not yet been a multi-target plant formula system that takes into account anti-dandruff, anti-inflammatory, and barrier repair, and there is also a lack of a solution for dual regulation of the scalp microecology and physical barrier.

[0005] Chinese invention patent CN113876646A discloses a traditional Chinese medicine anti-dandruff and anti-itch agent for scalp health conditioning. Its main ingredients include Angelica sinensis, Platycladus orientalis leaf, Sophora flavescens, Gleditsia sinensis, Ganoderma lucidum, Rheum palmatum, Polygonum multiflorum, and Curcuma longa, and their ratios are: 22-25 parts of Angelica sinensis, 20-25 parts of Platycladus orientalis leaf, 6-9 parts of Sophora flavescens, 7-11 parts of Gleditsia sinensis, 2-5 parts of Ganoderma lucidum, 3-6 parts of Rheum palmatum, 1-4 parts of Polygonum multiflorum, and 3-7 parts of Curcuma longa. In this formula, Rheum palmatum has the effects of inhibiting bacteria and relieving itching, Gleditsia sinensis can remove scalp oil; Platycladus orientalis leaf can cool the blood and reduce inflammation, effectively controlling oil; Sophora flavescens inhibits Malassezia. While relieving itching, reducing inflammation and sterilization, and reducing oil and inhibiting bacteria, Angelica sinensis and Ganoderma lucidum can penetrate into the hair follicles, nourish the scalp and hair follicles, and Curcuma longa accelerates metabolism and promotes nutrient absorption. This formula can not only effectively remove dandruff and relieve itching, but also nourish the hair roots and scalp, enable the hair to fully absorb nutrients, and improve the scalp health condition.

[0006] Chinese invention patent CN115245476A discloses a composition for removing dandruff, relieving itching and improving the scalp microenvironment and its preparation method. The components include water, compound plant extracts, alcohols, antibacterial substances and auxiliaries. The compound plant extracts are composed of Sophora flavescens root extract, Phellodendron amurense bark extract, Cnidium monnieri fruit extract, Dictamnus dasycarpus root bark extract, Zanthoxylum bungeanum pericarp extract, Cinnamomum camphora leaf extract, Mentha arvensis leaf extract. Through the synergistic effect among the components, the formation of various bacteria is inhibited, dandruff and itching are prevented, and at the same time, the microenvironment of scalp metabolism is regulated. This composition can be used not only in scalp oil control, dandruff removal and itching relief products, but also in products for inhibiting itching caused by bacteria.

[0007] Although the prior art has explored and achieved certain results in terms of dandruff removal and other effects, its components are complex, the interaction between components is unclear, resulting in waste of raw materials, and there is still room for improvement in terms of improving dandruff and scalp repair effects and the streamlined compatibility of raw materials. Summary of the Invention

[0008] In order to solve the deficiencies of the prior art, the present invention provides a plant anti-dandruff formula with scalp barrier repair function and its preparation and application. To achieve the above technical objectives, the present invention provides the following technical solutions:

[0009] A plant anti-dandruff formula with scalp barrier repair function, the plant anti-dandruff formula includes the following components: sebum inhibitor, surfactant, fish oil glycerol mixed esters, hydrogenated polydecene, caprylic / capric glycerides; the sebum inhibitor includes Sophora flavescens extract, Cynanchum atratum extract and Centella asiatica root extract; the preparation method of the Cynanchum atratum extract includes soaking Cynanchum atratum in a mixed solvent of lecithin, polyglycerol oleate and water, extracting, and concentrating to obtain.

[0010] Preferably, the mass ratio of lecithin, polyglycerol oleate and water is 0.01 - 0.05: 0.01 - 0.05: 1.

[0011] The application research of surfactants in the extraction of traditional Chinese medicines is developing rapidly towards precision, greenness and intelligence. By reducing the liquid-solid interface tension and forming a micelle solubilization effect, it can improve the extraction efficiency of lipophilic components (such as flavonoids, alkaloids). For example, the combined use of Tween-80 and β-cyclodextrin increases the yield of total flavonoids in Ginkgo biloba leaves to 92.5%, while the new pH-responsive surfactant (such as lauric acid-histidine complex) can target the release of target components to reduce the dissolution of impurities.

[0012] However, surfactants still have the problem of residual toxicity. Developing extraction methods with low toxicity and high extraction efficiency has become a research hotspot. After strict screening, the present invention selects lecithin and polyglyceryl oleate to extract Cynanchum atratum Bge. Combining lecithin and the green and environmentally friendly polyglyceryl oleate for extraction can synergistically promote the dissolution of the active components of Cynanchum atratum Bge. and enhance the hypoglycemic effect. In addition, polyglyceryl oleate can also be used as an emulsifier for cosmetics in the later stage to improve its stability.

[0013] Preferably, the soaking time is 30 min - 2 h, the extraction temperature is 65 - 80 °C, the number of extractions is 1 - 3 times, and the extraction time for each time is 0.5 - 2 h.

[0014] Preferably, the preparation method of the Sophora flavescens extract comprises the following steps:

[0015] (1) Soak the Sophora flavescens roots in water, carry out enzymatic hydrolysis, and filter to obtain an enzymatic hydrolysate;

[0016] (2) Boil the enzymatic hydrolysate to inactivate enzymes and sterilize, add probiotic bacterial liquid for fermentation, filter, and concentrate to obtain.

[0017] Preferably, the enzymes used in the enzymatic hydrolysis in step (1) are cellulase and xylanase with a mass ratio of 3 - 6:1.

[0018] Preferably, in step (1), the temperature of the enzymatic hydrolysis is 45 - 50 °C, the pH of the enzymatic hydrolysis is 4.0 - 8.5, and the time of the enzymatic hydrolysis is 0.5 - 2 h.

[0019] Preferably, the probiotic bacterial liquid in step (2) is Candida bombicola bacterial liquid, and its inoculation amount is 0.5 - 1.5% of the total volume of the enzymatic hydrolysate.

[0020] Candida bombicola is purchased from Ningbo Mingzhou Biotechnology Co., Ltd., and the product number is B80544. On the one hand, Candida bombicola can produce secondary metabolite sophorolipid compounds, which have functions such as detergency, wetting, emulsification, solubilization, dispersion, and foaming. In cosmetics and personal care products, it can be used as a multifunctional ingredient with unique biological activity and biocompatibility. On the other hand, research and comparison found that Candida bombicola can also ferment and biotransform the components of the Sophora flavescens root extract, further improving the activity of the Sophora flavescens root extract in inhibiting Malassezia in the formula of the present invention.

[0021] Preferably, the surfactant comprises polyquaternium - 61 and triheptanoin with a mass ratio of 0.1 - 0.5:10 - 20;

[0022] Preferably, the sebum inhibitor comprises Sophora flavescens extract, Cynanchum atratum Bge. extract, and Centella asiatica root extract with a mass ratio of 1:1:0.2 - 0.6;

[0023] Preferably, the anti-dandruff formulation further comprises a thickening agent and / or a solvent, the thickening agent is selected from hydrogenated polyisobutene, and the solvent is selected from propylene glycol.

[0024] Preferably, by weight, the plant anti-dandruff formulation comprises the following components:

[0025] Sebum inhibitor 0.2 - 1 part,

[0026] Surfactant 10 - 21 parts,

[0027] Fish oil glycerol mixed esters 10 - 30 parts,

[0028] Hydrogenated polydecene 5 - 15 parts,

[0029] Caprylic / capric glycerides 5 - 15 parts;

[0030] Hydrogenated polyisobutene 20 - 40 parts;

[0031] Propylene glycol 5 - 15 parts.

[0032] The second object of the present invention is to provide a preparation method of the above plant anti-dandruff formulation, comprising the following steps:

[0033] (1) Mix and stir to disperse the sebum inhibitor, polyquaternium-61, and caprylic / capric glycerides;

[0034] (2) Stir and dissolve the fish oil glycerol mixed esters, triheptanoin, hydrogenated polyisobutene, hydrogenated polydecene, and propylene glycol;

[0035] (3) Filter, detect, and package the finished product after passing the inspection.

[0036] The third object of the present invention is to provide the application of the described plant anti-dandruff formulation in the preparation of anti-dandruff and / or scalp care cosmetics, and the cosmetics are selected from shampoo, hair cream, hair conditioner, or scalp essence.

[0037] Compared with the prior art, the significant advantages shown by the present invention are as follows:

[0038] (1) The composition provided by the present invention, through careful selection and scientific proportioning, can effectively synergize among its components, significantly enhance the inhibitory effect on Malassezia, thereby achieving excellent anti-dandruff and itching-relieving effects, and simultaneously having a deep anti-inflammatory and moisturizing function.

[0039] (2) The active ingredients of the present invention are more refined, and the interaction among the ingredients is more significant. Through actual verification, it has a significant effect on improving scalp damage and can effectively repair the barrier function of the scalp, showing broad application potential.

[0040] (3) By deeply studying the preparation process of the extracts, the Sophora flavescens extract and Cynanchum atratum extract finally successfully prepared in the present invention, through the unique compatibility method of the present invention, their anti-inflammatory, moisturizing and antibacterial effects have been significantly enhanced. Detailed implementation manners

[0041] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as commonly used in the field to which the present invention belongs. For the purpose of interpreting this specification, the following definitions will be applied, and where appropriate, terms used in the singular form will also include the plural form, and vice versa.

[0042] The numerical ranges used herein should be understood to have enumerated all the numbers within that range. For example, the range of 1 to 20 should be understood to include any number, combination of numbers, or sub-range from the following group: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20.

[0043] The term "comprises" or "comprising" used herein means "including but not limited to". This term is intended to be open-ended, to specify the existence of any stated feature, element, integer, step, or component, but does not exclude the existence or addition of one or more other features, elements, integers, steps, components, or groups thereof. Thus, the term "comprising" includes the more restrictive terms "consisting of" and "consisting essentially of". In one embodiment, the term "comprising" used throughout the application, especially in the claims, may be replaced by the term "consisting of".

[0044] The term "and / or" used herein should be understood to mean any one of the alternatives or any combination of any two or more of the alternatives.

[0045] To make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the following further details the present invention in conjunction with embodiments. For those not specified in the embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. All reagents or instruments not specified by the manufacturer are conventional products that can be purchased commercially. To better illustrate the present invention, numerous specific details are given in the following detailed implementation manners. The specific embodiments described herein are only used to explain the present invention and do not constitute any limitation to the present invention. Among them, the suppliers of cellulase, xylanase and pectinase are Nanning Dongheng Huadao Biotechnology Co., Ltd., and the raw materials involved are shown in Table 1.

[0046] Table 1 Materials and reagents

[0047] Raw materials Trade name Manufacturer Centella asiatica root extract Centella asiatica root extract Guangxi Cangzhou Polyquaternium-61 Polyquaternium-61 Shanghai Aoli Trioctanoin Trioctanoin Olivamine Fish oil glycerol mixed ester Fish oil glycerol mixed ester Zhejiang Medicine Hydrogenated polydecene Hydrogenated polydecene Croda Caprylic / capric triglyceride Caprylic / capric triglyceride Shanghai Aoli Hydrogenated polyisobutene Hydrogenated polyisobutene ExxonMobil

[0048] Example 1

[0049] This example provides a plant anti-dandruff formula with a scalp barrier repair function. By weight, the raw materials of the composition are as follows:

[0050] Sebum inhibitor: 0.2 parts

[0051] Surfactant: 10 parts

[0052] Fish oil glycerol mixed esters: 10 parts

[0053] Hydrogenated polydecene: 5 parts

[0054] Caprylic / capric glycerides: 5 parts

[0055] Hydrogenated polyisobutene: 20 parts

[0056] Propylene glycol: 10 parts

[0057] The sebum inhibitor is a mixture of Sophora flavescens extract, Cynanchum atratum extract, and Centella asiatica root extract with a mass ratio of 1:1:0.2.

[0058] The surfactant is a mixture of polyquaternium-61 and triheptanoin with a mass ratio of 0.3:15.

[0059] Among them, the preparation method of Cynanchum atratum extract includes soaking Cynanchum atratum in a mixture solvent of lecithin, triglycerol oleate, and water with a mass ratio of 0.02:0.02:1 and 5 times the amount of Cynanchum atratum for 30 min, then extracting at 70 °C for 2 times, each extraction time is 1 h, filtering, combining the filtrates, and concentrating to a relative density of 1.10 to obtain.

[0060] The preparation method of Sophora flavescens extract is as follows:

[0061] (1) Soak Sophora flavescens roots in 5 times the amount of water for 30 min, add 0.05% of the mass of water of an enzyme (a mixture of cellulase and xylanase with a mass ratio of 4:1), mix evenly, enzymolyze at 45 °C and pH 4.5 for 2 h, and filter to obtain the enzymolyzed solution;

[0062] (2) After boiling and inactivating the enzyme in the enzymolyzed solution and cooling to room temperature, inoculate with Candida bombicola liquid at an inoculation amount of 1% (volume ratio), ferment at 30 °C for 24 h, filter, and concentrate to a relative density of 1.10 to obtain. Candida bombicola liquid includes inoculating Candida bombicola (Candida bombicola numbered ATCC 22214, purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product catalog number B80544) into a culture medium, fermenting at 30 °C for 12 h, filtering, and concentrating and drying to obtain. The culture medium composition is: glucose 1.0 g, peptone 1.0 g, yeast powder 0.5 g, glycerol 10 mL, pure water 90 mL.

[0063] Preparation method:

[0064] (1) Mix and stir to disperse the sebum inhibitor, polyquaternium-61, and caprylic / capric glycerides;

[0065] (2) Stir and dissolve the fish oil glyceride mixture, triheptanoin, hydrogenated polyisobutene, hydrogenated polydecene, and propylene glycol;

[0066] (3) Mix the systems of steps (1) and (2), filter, detect, and package the finished product after passing the inspection.

[0067] Example 2

[0068] This example provides a plant anti-dandruff formulation with a scalp barrier repair function. By weight, the raw materials of the composition are as follows:

[0069] 1 part of sebum inhibitor,

[0070] 21 parts of surfactant,

[0071] 30 parts of fish oil glyceride mixture,

[0072] 15 parts of hydrogenated polydecene,

[0073] 15 parts of caprylic / capric glycerides,

[0074] 40 parts of hydrogenated polyisobutene;

[0075] 10 parts of propylene glycol;

[0076] The sebum inhibitor includes sophora flavescens extract, cynanchum atratum extract, and centella asiatica root extract in a mass ratio of 1:1:0.6.

[0077] The surfactant is polyquaternium-61 and triheptanoin in a mass ratio of 0.5:20.

[0078] Among them, the preparation method of cynanchum atratum extract includes soaking cynanchum atratum in a mixture solvent of lecithin, polyglycerol oleate, and water with a mass ratio of 0.01:0.01:1 at 8 times the amount for 1 h, then extracting at 80 °C for 1 h, and concentrating to a relative density of 1.25 to obtain it.

[0079] The preparation method of sophora flavescens extract is as follows:

[0080] (1) Soak sophora flavescens root in 5 times the amount of water for 30 min, add 0.1% of the mass of water of enzyme (cellulase and xylanase with a mass ratio of 6:1), enzymolyze at 50 °C and pH 6.5 for 1 h, and filter to obtain the enzymolyzed solution;

[0081] (2) After boiling the enzymolysis solution to inactivate the enzymes and sterilize it, let it cool to room temperature. Inoculate with Candida bombi yeast solution at an inoculation amount of 1% (volume ratio), ferment at 30°C for 24 h, filter, concentrate and dry to obtain. The Candida bombi yeast solution is obtained by inoculating Candida bombi yeast (Candida bombi yeast, product catalog number is B80544, purchased from Ningbo Mingzhou Biotechnology Co., Ltd.) into a culture medium, fermenting at 30°C for 12 h, filtering, and concentrating to a relative density of 1.10. The culture medium composition is: 1.0 g of glucose, 1.0 g of peptone, 0.5 g of yeast powder, 10 mL of glycerol, and 90 mL of pure water.

[0082] Preparation method:

[0083] (1) Mix and stir to disperse the sebum inhibitor, polyquaternium-61, and caprylic / capric glycerides.

[0084] (2) Stir and dissolve the fish oil glycerol mixed esters, triheptanoin, hydrogenated polyisobutene, hydrogenated polydecene, and propylene glycol.

[0085] (3) Mix the systems of steps (1) and (2), filter, detect, and package the finished product after passing the inspection.

[0086] Example 3

[0087] This example provides a plant anti-dandruff formulation with a scalp barrier repair function. By weight, the raw materials of the composition are as follows:

[0088] 0.5 part of sebum inhibitor,

[0089] 15 parts of surfactant,

[0090] 20 parts of fish oil glycerol mixed esters,

[0091] 10 parts of hydrogenated polydecene,

[0092] 10 parts of caprylic / capric glycerides,

[0093] 30 parts of hydrogenated polyisobutene;

[0094] 10 parts of propylene glycol;

[0095] The sebum inhibitor includes sophora flavescens extract, cynanchum atratum extract, and centella asiatica root extract with a mass ratio of 1:1:0.4.

[0096] The surfactant is polyquaternium-61 and triheptanoin with a mass ratio of 0.1:10.

[0097] Among them, the preparation method of Cynanchum atratum extract includes soaking Cynanchum atratum in a mixture solvent of lecithin, polyglyceryl oleate and water with a mass ratio of 0.05:0.05:1 and 5 times the amount of Cynanchum atratum for 1 h, then extracting at 65 °C for 3 times, with the extraction time of 0.5 h each time, filtering, combining the filtrates, and concentrating to a relative density of 1.10 to obtain the extract.

[0098] The preparation method of Sophora flavescens extract is as follows:

[0099] (1) Soak Sophora flavescens roots in 5 times the amount of water for 30 min, add 0.1% of the mass of water of enzyme (cellulase and xylanase with a mass ratio of 3:1), enzymolyze at 50 °C and pH 5 for 2 h, and filter to obtain the enzymolyzed solution;

[0100] (2) After boiling the enzymolyzed solution to inactivate the enzyme and sterilize, cool it to room temperature, inoculate Candida bombicola liquid at an inoculation amount of 1% (volume ratio), ferment at 30 °C for 24 h, filter, and concentrate and dry to obtain the extract. Candida bombicola liquid includes inoculating Candida bombicola (Candida bombicola, product number B80544, purchased from Ningbo Mingzhou Biotechnology Co., Ltd.) into a culture medium, fermenting at 30 °C for 12 h, filtering, and concentrating to a relative density of 1.10. The composition of the culture medium is: 1.0 g of glucose, 1.0 g of peptone, 0.5 g of yeast powder, 10 mL of glycerol, and 90 mL of pure water.

[0101] Preparation method:

[0102] (1) Mix and stir the sebum inhibitor, polyquaternium-61, and caprylic / capric glycerides to disperse them;

[0103] (2) Stir and dissolve the fish oil glyceride mixture, triheptanoin, hydrogenated polyisobutene, hydrogenated polydecene, and propylene glycol;

[0104] (3) Mix the systems of steps (1) and (2), filter, detect, and package the finished product after passing the inspection.

[0105] Comparative Example 1

[0106] The difference between this comparative example and Example 1 is that the preparation method of Cynanchum atratum extract is different. The specific extraction mixed solvent is polyglyceryl oleate and water with a mass ratio of 0.04:1.

[0107] Comparative Example 2

[0108] The difference between this comparative example and Example 1 is that the preparation method of Cynanchum atratum extract is different. The specific extraction mixed solvent is lecithin and water with a mass ratio of 0.04:1.

[0109] Comparative Example 3

[0110] The difference between this comparative example and Example 1 lies in the different preparation methods of the Sophora flavescens extract. Specifically, for enzymatic hydrolysis, cellulase and pectinase with a mass ratio of 4:1 are used.

[0111] Comparative Example 4

[0112] The difference between this comparative example and Example 1 lies in the different preparation methods of the Sophora flavescens extract. Specifically, the strain in step (3) is different, specifically Saccharomyces cerevisiae CGMCC 2.3880, which is purchased from the China General Microbiological Culture Collection Center.

[0113] Comparative Example 5

[0114] The difference between this comparative example and Example 1 lies in the different surfactants, specifically polyquaternium-28.

[0115] Test Example 1 Antifungal Rate Test of Malassezia furfur

[0116] The present invention follows the QB / T2738-2012 Evaluation Standard for Antibacterial and Antifungal Effects of Daily Chemical Products (Suspension Quantitative Method) to accurately test the antifungal effect of the 5% concentration composition on Malassezia furfur. In the experiment, the test sample was diluted to a mass concentration of 5% with sterile standard hard water. 5.0 mL of the above-mentioned test sample dilution was pipetted into a sterilized test tube and incubated at 20 °C for 5 min; 0.1 mL of the Malassezia furfur spore suspension was added to the test tube containing 5.0 mL of the sample, quickly mixed, and timing was started immediately; after 24 hours of incubation, 0.5 mL of the test bacteria and sample mixture was taken and added to a test tube containing 4.5 mL of sterilized PBS, and mixed well; and 10 2 、10 3 times dilution was carried out with PBS. Subsequently, 1 mL of the sample solution was pipetted into two petri dishes respectively, the Malassezia furfur medium was added, and after uniform mixing, it was continued to be cultured for 5 days for viable cell counting. The calculation formula for the antifungal rate is as follows:

[0117] The calculation formula for the antifungal rate is as follows:

[0118]

[0119] X - antifungal rate %, A - average colony count of the negative control, B - average colony count of the test sample;

[0120] The experiment was repeated 3 times and the average value was calculated. The specific test results are shown in Table 2.

[0121] Table 2 Antifungal Rate Test Results

[0122] Group Bacteriostatic rate (%) Negative control group 0％ Positive group 99.08** Example 1 <![CDATA[99.75 ** > Example 2 <![CDATA[99.31 ** > Example 3 <![CDATA[98.10 ** > Comparative example 1 75.12# Comparative example 2 53.96## Comparative example 3 86.70# Comparative example 4 69.46## Comparative example 5 92.19

[0123] Note: Compared with the negative control group, in the positive group and Examples 1-3, *P<0.05, **P<0.01. Compared with Example 1, #P<0.05, ##P<0.01.

[0124] Test Example 2 Consumer Use Evaluation

[0125] According to the relevant requirements of the "Cosmetics Efficacy Claim Evaluation Specification", referring to the relevant requirements in T / TDCA 006—2022 "Cosmetics Consumer Use Test - Questionnaire Evaluation Guide" and "Cosmetics Efficacy Evaluation (ⅩⅢ) - Consumer Use Test", a questionnaire was designed.

[0126] 64 volunteers with scalp inflammation and / or severe itching and a large amount of dandruff were found and used for 2 weeks. The volunteers were divided into 8 groups, namely Example 1-3 groups and Comparative Example 1-5 groups. Age, gender, and scalp symptoms were considered during grouping to ensure that there was no statistical significance in the comparison of the 8 groups of data.

[0127] The anti-dandruff formulations prepared in Examples 1-3 and Comparative Examples 1-5 were incorporated into the basic shampoo formulation at a ratio of 5%. Wash twice a week, 5-10 mL each time, adjusted according to hair volume. After 2 weeks of use, the effects of each experimental group in improving the scalp condition and reducing dandruff were recorded respectively. The basic shampoo formulation is as follows: sodium lauryl polyether sulfate (SLES) content 10%, cocamidopropyl betaine (CAPB) content 3%, sodium chloride 0.5%, phenoxyethanol 0.5%, appropriate citric acid to adjust the pH to 5.5, disodium EDTA 0.1%, and the balance is water.

[0128] The effect criteria are as follows:

[0129] Cured: The scalp returns to normal, itching and inflammation subside, no dandruff and no recurrence for one week, and the sensitivity and itching caused by the thinning of the scalp skin due to aging disappear.

[0130] Marked effect: The oil secretion is balanced, the itching sensation is significantly reduced, and the dandruff is reduced by half.

[0131] Effective: There is a slight improvement in dandruff, and the inflammation and itching are slightly improved compared with before.

[0132] Ineffective: No effect, or the improvement of itching and inflammation is not obvious.

[0133] The results of the scalp improvement of the composition of the present invention are shown in Table 3.

[0134] Table 3 Distribution Table of Scalp and Dandruff Improvement

[0135]

[0136] It can be seen from the results that the composition of the present invention has very significant effects in improving scalp allergies, inflammation, and symptoms such as red bumps, itching, and dandruff. Among them, the formulation of Example 1 is the best. After using it for 2 weeks, the scalp can be restored to the normal level, the scalp barrier can be repaired, the damaged state can be improved, and at the same time, the moisturizing effect is also very good, and conditions such as itching and dandruff can be improved. Comparative Examples 1-5 adopted other screened and eliminated preparation methods, and their improvement effects were not good.

[0137] In addition, in this study, a transdermal water loss meter was used to accurately measure the TEWL (Trans Epidermal Water Loss) of the same corresponding area on the top of the scalp of the subjects. The subjects had to sit quietly in a constant temperature room at 22±1°C and a humidity of 50±5% RH for 30 minutes to reach a stable state. At the same time, the subjects were required not to use any skin care products or cleansers within two hours before the test. To ensure the measurement accuracy, the probe ( TM300) needs to be calibrated with a standard calibration plate before use. The specific data are shown in Table 4.

[0138] Table 4 Test results of TEWL values

[0139]

[0140] It can be seen from the results that after continuously using the products prepared in Examples 1-3 and Comparative Example 5 of the present invention, the TEWL values of their scalps are lower, and the effects of the compositions of Examples 1-2 are better, indicating that it can enable the scalp to obtain better skin barrier function, is effective in repairing skin barrier damage, and has moderate sebum secretion and proper care.

[0141] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than a limitation on the protection scope of the present invention. Any simple modification or equivalent replacement of the technical solution of the present invention by those of ordinary skill in the art shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A plant anti-dandruff formula with scalp barrier repair function, characterized in that: The plant anti-dandruff formula comprises the following components: a sebum inhibitor, a surfactant, fish oil glycerol mixed esters, hydrogenated polydecene, and caprylic / capric glycerides; the sebum inhibitor comprises a Sophora flavescens extract, a Pleurotus eryngii extract, and a Centella asiatica root extract; and the preparation method of the Pleurotus eryngii extract comprises soaking the Pleurotus eryngii in a mixed solvent of lecithin, triglycerol oleate, and water, extracting, and concentrating.

2. The plant anti-dandruff formula according to claim 1, characterized in that: The mass ratio of the lecithin, triglycerol oleate and water is 0.01-0.05:0.01-0.05:

1.

3. The plant anti-dandruff formula according to claim 1, characterized in that: The soaking time is 30 minutes to 2 hours, the extraction temperature is 65-80° C., the number of extractions is 1-3 times, and the time of each extraction is 0.5-2 hours.

4. The plant anti-dandruff formula according to claim 1, characterized in that: The preparation method of the Sophora flavescens extract comprises the following steps: (1) Soaking the root of Sophora flavescens in water, performing enzymolysis, and filtering to obtain an enzymolysis solution; (2) Boil the enzymatic hydrolysate to inactivate enzymes and kill bacteria, add probiotics solution for fermentation, filter, and concentrate to obtain the product.

5. The plant anti-dandruff formula according to claim 4, characterized in that: The enzymes used for the enzymolysis in step (1) are cellulase and xylanase in a mass ratio of 3-6:

1.

6. The plant anti-dandruff formula according to claim 4, characterized in that: The enzymatic hydrolysis temperature in step (1) is 45-50° C., the enzymatic hydrolysis pH is 4.0-8.5, and the enzymatic hydrolysis time is 0.5-2 h. And / or, the probiotic bacterial solution in step (2) is a bumblebee Candida solution, and its inoculation amount is 0.5-1.5% of the total volume of the enzymatic hydrolyzate.

7. The plant anti-dandruff formula according to claim 1, characterized in that: The surfactant comprises polyquaternium-61 and triheptanol in a mass ratio of 0.1-0.5:10-20; And / or, the sebum inhibitor comprises Sophora flavescens extract, Psoralea corylifolia extract and Centella asiatica root extract in a mass ratio of 1:1:0.2-0.6; And / or, the anti-dandruff formulation further comprises a thickener and / or a solvent, wherein the thickener is selected from hydrogenated polyisobutylene, and the solvent is selected from propylene glycol.

8. The plant anti-dandruff formula according to any one of claims 1 to 7, characterized in that: The plant anti-dandruff formula comprises the following components by weight: Sebum inhibitor 0.2-1 part, 10-21 parts of surfactant, 10-30 parts of fish oil glycerol mixed esters, 5-15 parts of hydrogenated polydecene, 5-15 parts of caprylic / capric glycerides; 20-40 parts of hydrogenated polyisobutylene; Propylene glycol 5-15 parts.

9. A method for preparing the plant anti-dandruff formula according to any one of claims 1 to 8, characterized in that: The steps include: (1) mixing and dispersing a sebum inhibitor, polyquaternium-61, and caprylic / capric glycerides; (2) stirring and dissolving fish oil glycerol mixed esters, triheptyl, hydrogenated polyisobutylene, hydrogenated polydecene, and propylene glycol; (3) Filter, test and package the finished products after they pass the test.

10. Use of the plant anti-dandruff formulation according to any one of claims 1 to 8 in the preparation of anti-dandruff and / or scalp care cosmetics, characterized in that: The cosmetic is selected from shampoo, shampoo cream, hair conditioner or scalp essence.

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