Acne-removing composition containing ceramide, preparation method of acne-removing composition and acne-removing product

Abstract

The invention provides an acne-removing composition containing ceramide, a preparation method of the acne-removing composition and an acne-removing product, and the acne-removing composition is prepared from the following components in parts by weight: 5 to 10 parts of plant extract, 1 to 3 parts of ceramide, 1 to 3 parts of gentiopicroside-vitamin C eutectic, 1 to 3 parts of compound polysaccharide and 0.3 to 0.7 part of transdermal enhancer. The plant extract is prepared from a herba dendrobii fruticosae flower extract, a lasiocarpus trifoliatus extract and a hedera rhododendron extract in a mass ratio of 1: (1-3): (2-4); the compound polysaccharide is prepared from tremella polysaccharide, tamarind seed polysaccharide and ganoderma lucidum polysaccharide in a ratio of 1: (3-5): (0.5-0.8); the transdermal enhancer is prepared from a cucumber extract and a salvia miltiorrhiza root extract in a mass ratio of 1: (1-3). The plant extracts in the acne-removing composition take effect synergistically, the acne-removing effect is achieved from multiple aspects of bacteriostasis, anti-inflammation and oil control, the purpose of efficiently removing acnes without relapse is achieved, and the natural plant extracts are mild and non-irritant, do not cause burden to the skin and have good application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and particularly relates to an anti-acne composition containing ceramide, a preparation method thereof, and an anti-acne product. Background Art

[0002] With the acceleration of the pace of life and the increase of social pressure, the problem of acne has become an important factor affecting people's appearance and quality of life. Acne, also known as "pimples", is the most common skin disease. If acne is not treated for a long time or simply and roughly squeezed, it will cause swelling, infection, and even ulceration in severe cases, resulting in scars on the patient and even affecting their psychological growth.

[0003] At present, there are a wide variety of anti-acne products on the market, but their effects vary. Moreover, most products contain a lot of chemical additives, which themselves have certain side effects on the human skin and are highly irritating. Some products even use hormones and antibiotics. Although they are effective quickly, they are prone to drug resistance problems, and long-term use can form skin-dependent dermatitis.

[0004] At present, there are also some natural combination anti-acne products, but their functions are relatively single, the effects are often not satisfactory, they are prone to cause skin damage, and the effect on repairing acne marks is insufficient.

[0005] Therefore, it is of practical significance to develop a formula that is mild and non-irritating, highly effective in anti-acne without recurrence, and can repair acne marks. Summary of the Invention

[0006] In view of this, the present invention provides an anti-acne composition containing ceramide, a preparation method thereof, and an anti-acne product.

[0007] The technical solution of the present invention is realized as follows:

[0008] An anti-acne composition containing ceramide, by weight, comprises the following raw materials: 5-10 parts of plant extract, 1-3 parts of ceramide, 1-3 parts of gentiopicroside-vitamin C cocrystal, 1-3 parts of complex polysaccharide, and 0.3-0.7 part of transdermal enhancer;

[0009] The plant extract is a mixture of dendrobium phalaenopsis flower extract, epimedium extract, and hedera rhombea extract with a mass ratio of 1:1 - 3:2 - 4;

[0010] The complex polysaccharide is a mixture of tremella polysaccharide, tamarind seed polysaccharide, and ganoderma lucidum polysaccharide with a ratio of 1:3 - 5:0.5 - 0.8;

[0011] The transdermal enhancer is a mixture of cucumber extract and salvia miltiorrhiza root extract with a mass ratio of 1:1 - 3.

[0012] Further, the preparation method of the gentiopicroside-vitamin C cocrystal includes: adding gentiopicroside and vitamin C into n-hexane, stirring to form a suspension, continuously stirring for 24 - 48 h, evaporating the solvent, and drying under vacuum to obtain the gentiopicroside-vitamin C cocrystal;

[0013] The molar ratio of gentiopicroside to vitamin C is 1:2 - 4; the solid-liquid ratio of vitamin C to n-hexane is 1:30 - 40 g / mL.

[0014] Further, the preparation method of the Dendrobium phalaenopsis flower extract includes: pulverizing and drying the Dendrobium phalaenopsis flower to form a powder, adding the powder into a deep eutectic solvent, extracting by ultrasonic wave, centrifuging, and concentrating under reduced pressure to obtain the Dendrobium phalaenopsis flower extract;

[0015] The material-liquid ratio of the Dendrobium phalaenopsis flower to the deep eutectic solvent is 1:8 - 10 g / mL; the deep eutectic solvent is prepared by adding choline chloride as a hydrogen bond acceptor and D-xylose as a hydrogen bond donor with a molar ratio of 1:2 into ultrapure water, wherein the solid-liquid ratio of choline chloride to ultrapure water is 1:8 - 10 mg / mL; the ultrasonic extraction is carried out at 20 - 30 kHz and 40 - 60 °C for 4 - 6 h.

[0016] Further, the preparation method of the Epimedium sagittatum extract includes: pulverizing Epimedium sagittatum, adding it into absolute ethanol for extraction, concentrating the extract under reduced pressure to form an extract paste, dispersing the extract paste in water, successively extracting with n-hexane, chloroform, ethyl acetate, and n-butanol, retaining the chloroform phase and the ethyl acetate phase, concentrating under reduced pressure, and freeze-drying to obtain the Epimedium sagittatum extract;

[0017] The material-liquid ratio of Epimedium sagittatum to absolute ethanol is 1:10 - 12 g / mL; the number of extraction times is 2 - 3 times, 40 - 60 min each time, and the temperature is 50 - 70 °C; the material-liquid ratio of the extract paste to water is 1:6 - 8 g / mL; the addition amounts of n-hexane, ethyl acetate, and n-butanol are all 5 - 7 times the weight of the extract paste; the number of extraction times is 3 times, 30 - 40 min each time.

[0018] Further, the preparation method of the Hedera rhombea extract includes: pulverizing Hedera rhombea, adding it into an aqueous two-phase solvent for extraction, and concentrating the extract under reduced pressure to obtain the Hedera rhombea extract;

[0019] The material-liquid ratio of Hedera rhombea to the aqueous two-phase solvent is 1:10 - 15 g / mL; the aqueous two-phase solvent is an alcohol solution with a volume ratio of 1:1 - 3 and a concentration of 40 - 50 wt% and a salt solution with a concentration of 10 - 20 wt%; the extraction temperature is 60 - 70 °C and the time is 2 - 4 h.

[0020] Further, the preparation method of the cucumber extract includes: repeatedly freezing and thawing cucumbers, mashing them, adding deionized water, extracting by microwave, and concentrating under reduced pressure to obtain the cucumber extract;

[0021] The material-liquid ratio of the cucumber to deionized water is 1:20 - 30 g / mL; the microwave extraction is carried out at 400 - 500 W and 60 - 80 °C for 3 - 5 h.

[0022] Further, the preparation method of the salvia root extract includes: cutting the salvia root into pieces and pulping, adding it to an alkaline solution with a pH value of 8.5 - 9.5 and stirring to obtain the salvia root extract;

[0023] The mass ratio of the salvia root to the alkaline solution is 1:8 - 12; the stirring treatment is carried out at 100 - 120 °C and 0.8 - 1.2 MPa for 5 - 10 min.

[0024] A preparation method of an acne - removing composition containing ceramide, the specific steps are: subjecting plant extract, ceramide, complex polysaccharide and transdermal enhancer to high - pressure homogenization or micro - jet treatment, and then adding gentiopicroside - vitamin C cocrystal and continuing to stir to obtain the acne - removing composition;

[0025] The pressure of the high - pressure homogenization or micro - jet treatment is 800 - 1000 bar, the temperature is 40 - 50 °C, and the time is 30 - 40 min; the stirring time is 10 - 20 min.

[0026] An acne - removing product contains the acne - removing composition containing ceramide as described above.

[0027] Further, the concentration of the acne - removing composition is 5 - 10 wt%.

[0028] Compared with the prior art, the beneficial effects of the present invention are:

[0029] 1. The plant extracts in the acne - removing composition of the present invention work synergistically, achieving the acne - removing effect from multiple aspects of antibacterial, anti - inflammatory and oil control, achieving high - efficiency acne removal without recurrence, and the natural plant extracts are mild and non - irritating, not causing burden on the skin.

[0030] 2. The plant extracts of the present invention are supplemented with ceramide, gentiopicroside - vitamin C cocrystal and complex polysaccharide. Through reasonable compounding, different active ingredients act together to promote rapid keratin metabolism. It can not only improve the acne - removing effect and stability of the acne - removing composition, reduce the recurrence rate of acne, but also achieve the effect of repairing acne marks, reducing pigmentation and scar formation after acne healing, and making the skin return to a healthy state.

[0031] 3. The transdermal enhancer of the present invention uses cucumber extract and Salvia miltiorrhiza root extract, and the natural ingredients can gently and efficiently enhance the skin's absorption of the anti-acne composition, enhancing the anti-acne effect. Detailed implementation manners

[0032] To better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0033] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0034] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can all be obtained from commercial channels.

[0035] The anti-acne product of the present invention can be any one of a patch, a film, a poultice, a spray or an ointment, which contains the anti-acne composition of the present invention, and its concentration is 5-10 wt%.

[0036] Example 1

[0037] An anti-acne composition containing ceramide, by weight, includes the following raw materials: 8 parts of plant extract, 2 parts of ceramide, 2 parts of gentiopicroside-vitamin C cocrystal, 2 parts of complex polysaccharide, 0.5 part of transdermal enhancer;

[0038] Among them, the plant extract is a mixture of Dendrobium phalaenopsis flower extract, Epimedium sagittatum extract and Hedera rhombea extract in a mass ratio of 1:2:3; the complex polysaccharide is a mixture of tremella polysaccharide, tamarind seed polysaccharide and ganoderma polysaccharide in a ratio of 1:4:0.7; the transdermal enhancer is a mixture of cucumber extract and Salvia miltiorrhiza root extract in a mass ratio of 1:2.

[0039] Preparation of gentiopicroside-vitamin C cocrystal: Add gentiopicroside and vitamin C in a molar ratio of 1:3 to n-hexane, and the solid-liquid ratio of vitamin C to n-hexane is 1:35 g / mL. Stir to make a suspension, continuously stir for 48 h, evaporate the solvent, and vacuum dry to obtain gentiopicroside-vitamin C cocrystal;

[0040] Preparation of Dendrobium phalaenopsis flower extract: Crush and dry the Dendrobium phalaenopsis flowers to form a powder. According to a solid-liquid ratio of 1:9 g / mL, add the powder to a deep eutectic solvent (prepared by adding choline chloride as a hydrogen bond acceptor and D-xylose as a hydrogen bond donor in a molar ratio of 1:2 to ultrapure water, and the solid-liquid ratio of choline chloride to ultrapure water is 1:9 mg / mL), and perform ultrasonic extraction at 25 kHz and 50 °C for 5 h, then centrifuge and concentrate under reduced pressure to obtain Dendrobium phalaenopsis flower extract;

[0041] Preparation of Epimedium brevicornum Maxim extract: Epimedium brevicornum Maxim was crushed and added to absolute ethanol according to a solid-liquid ratio of 1:11 g / mL. It was extracted twice at 60 °C for 50 min each time. The extract was concentrated under reduced pressure to form an extract paste. According to a ratio of 1:7 g / mL, the extract paste was added to water for dispersion, and then extracted successively with n-hexane, chloroform, ethyl acetate, and n-butanol. The addition amounts of n-hexane, ethyl acetate, and n-butanol were all 6 times the weight of the extract paste, and each extraction was carried out 3 times for 35 min each time. The chloroform phase and ethyl acetate phase were retained, concentrated under reduced pressure, and freeze-dried to obtain Epimedium brevicornum Maxim extract;

[0042] Preparation of Hedera rhombea extract: Hedera rhombea was crushed and added to an aqueous two-phase solvent according to a solid-liquid ratio of 1:13 g / mL. The aqueous two-phase solvent was a 45 wt% alcohol solution and a 15 wt% salt solution with a volume ratio of 1:2. It was extracted at 65 °C for 3 h, and the extract was concentrated under reduced pressure to obtain Hedera rhombea extract;

[0043] Preparation of cucumber extract: Cucumber was repeatedly frozen and thawed and then mashed. It was added to deionized water according to a solid-liquid ratio of 1:25 g / mL and extracted by microwave at 450 W and 70 °C for 4 h. The extract was concentrated under reduced pressure to obtain cucumber extract;

[0044] Preparation of Salvia miltiorrhiza root extract: The Salvia miltiorrhiza root was cut into pieces and pulped, and then added to an alkaline solution with a pH value of 9.0 ± 0.5 and stirred. The mass ratio of Salvia miltiorrhiza root to the alkaline solution was 1:10, and it was treated at 110 °C and 1.0 MPa for 8 min to obtain Salvia miltiorrhiza root extract;

[0045] The preparation method of the above acne treatment composition containing ceramide specifically comprises the following steps: The plant extract, ceramide, complex polysaccharide, and transdermal enhancer are treated by high-pressure homogenization or microfluidization for 35 min at a pressure of 900 bar and a temperature of 45 °C, and then the gentiopicroside-vitamin C cocrystal is added and stirred for another 15 min to obtain the acne treatment composition.

[0046] Example 2

[0047] An acne treatment composition containing ceramide, according to weight parts, comprises the following raw materials: 5 parts of plant extract, 1 part of ceramide, 1 part of gentiopicroside-vitamin C cocrystal, 1 part of complex polysaccharide, and 0.3 part of transdermal enhancer;

[0048] Wherein the plant extract is an extract of Dendrobium phalaenopsis flowers, Epimedium brevicornum Maxim extract, and Hedera rhombea extract with a mass ratio of 1:1:2; the complex polysaccharide is tremella polysaccharide, tamarind seed polysaccharide, and ganoderma polysaccharide with a ratio of 1:3:0.5; the transdermal enhancer is a mixture of cucumber extract and Salvia miltiorrhiza root extract with a mass ratio of 1:1.

[0049] Preparation of gentiopicroside-vitamin C cocrystal: Gentiopicroside and vitamin C with a molar ratio of 1:2 were added to n-hexane. The solid-liquid ratio of vitamin C to n-hexane was 1:30 g / mL. It was stirred to form a suspension, continuously stirred for 24 h, the solvent was evaporated, and vacuum dried to obtain gentiopicroside-vitamin C cocrystal;

[0050] Preparation of Dendrobium phalaenopsis flower extract: The Dendrobium phalaenopsis flowers were crushed and dried to form a powder. According to the solid-liquid ratio of 1:8 g / mL, the powder was added to a deep eutectic solvent (prepared by adding choline chloride as a hydrogen bond acceptor and D-xylose as a hydrogen bond donor with a molar ratio of 1:2 to ultrapure water, and the solid-liquid ratio of choline chloride to ultrapure water was 1:8 mg / mL). It was ultrasonically extracted at 20 kHz and 40 °C for 4 h, centrifuged, and concentrated under reduced pressure to obtain Dendrobium phalaenopsis flower extract;

[0051] Preparation of Epimedium sagittatum extract: Epimedium sagittatum was crushed. According to the solid-liquid ratio of 1:10 g / mL, it was added to anhydrous ethanol and extracted at 50 °C for 2 times, 40 min each time. The extract was concentrated under reduced pressure to form an extract paste. According to the ratio of 1:6 g / mL, the extract paste was added to water for dispersion, and successively extracted with n-hexane, chloroform, ethyl acetate and n-butanol. The addition amounts of n-hexane, ethyl acetate and n-butanol were all 5 times the weight of the extract paste, and extracted 3 times, 30 min each time. The chloroform phase and ethyl acetate phase were retained, concentrated under reduced pressure, and freeze-dried to obtain Epimedium sagittatum extract;

[0052] Preparation of Hedera rhombea extract: Hedera rhombea was crushed. According to the solid-liquid ratio of 1:10 g / mL, it was added to an aqueous two-phase solvent, which was a 40 wt% alcohol solution and a 10 wt% salt solution with a volume ratio of 1:1. It was extracted at 60 °C for 2 h, and the extract was concentrated under reduced pressure to obtain Hedera rhombea extract;

[0053] Preparation of cucumber extract: The cucumber was repeatedly frozen and thawed and then mashed. According to the solid-liquid ratio of 1:20 g / mL, it was added to deionized water and microwave extracted at 400 W and 60 °C for 3 h, and concentrated under reduced pressure to obtain cucumber extract;

[0054] Preparation of Salvia miltiorrhiza root extract: The Salvia miltiorrhiza root was cut into pieces and pulped, added to an alkaline solution with a pH value of 9.0 ± 0.5 and stirred. The mass ratio of Salvia miltiorrhiza root to the alkaline solution was 1:8, and it was treated at 100 °C and 0.8 MPa for 5 min to obtain Salvia miltiorrhiza root extract;

[0055] The preparation method of the above acne removing composition containing ceramide specifically comprises the following steps: The plant extract, ceramide, complex polysaccharide and penetration enhancer are treated by high-pressure homogenization or microfluidization for 30 min, the pressure is 800 bar, and the temperature is 40 °C. Then, gentiopicroside-vitamin C cocrystal is added and stirred for another 10 min to obtain the acne removing composition.

[0056] Example 3

[0057] An anti-acne composition containing ceramide, comprising the following raw materials in parts by weight: 10 parts of plant extract, 3 parts of ceramide, 3 parts of gentiopicroside-vitamin C eutectic, 3 parts of complex polysaccharide, and 0.7 parts of transdermal enhancer;

[0058] The plant extracts are Dendrobium officinale flower extract, Clerodendrum ternata extract and Hedera helix extract in a mass ratio of 1:3:4; the complex polysaccharides are Tremella fuciformis polysaccharide, Tamarindus indica seed polysaccharide and Ganoderma lucidum polysaccharide in a mass ratio of 1:5:0.8; and the transdermal enhancers are Cucumber extract and Salvia miltiorrhiza root extract in a mass ratio of 1:3.

[0059] Preparation of gentiopicroside-vitamin C eutectic: add gentiopicroside and vitamin C in a molar ratio of 1:4 to n-hexane, the solid-liquid ratio of vitamin C to n-hexane is 1:40 g / mL, stir to form a suspension, continue stirring for 48 hours, evaporate the solvent, and vacuum dry to obtain gentiopicroside-vitamin C eutectic;

[0060] Preparation of Dendrobium officinale flower extract: Grind and dry the Dendrobium officinale flower to form a powder, add the powder to a deep eutectic solvent (prepared by adding choline chloride, a hydrogen bond acceptor, and D-xylose, a hydrogen bond donor, to ultrapure water at a molar ratio of 1:2, and the solid-liquid ratio of choline chloride to ultrapure water is 1:10 mg / mL) at a solid-liquid ratio of 1:10 mg / mL, perform ultrasonic extraction at 30 kHz and 60° C. for 6 h, centrifuge, and concentrate under reduced pressure to obtain the Dendrobium officinale flower extract;

[0061] Preparation of Herba Cynoglossifolia Extract: Crush Herba Cynoglossifolia, add it to anhydrous ethanol at a solid-liquid ratio of 1:12 g / mL, extract it 3 times at 70°C, each time for 60 min, concentrate the extract under reduced pressure to form an extract, add the extract into water at a ratio of 1:8 g / mL for dispersion, extract it with n-hexane, chloroform, ethyl acetate and n-butanol in sequence, the amount of n-hexane, ethyl acetate and n-butanol added is 7 times the weight of the extract, extract it 3 times, each time for 40 min, retain the chloroform phase and the ethyl acetate phase, concentrate under reduced pressure, and freeze-dry to obtain the Herba Cynoglossifolia Extract;

[0062] Preparation of Hedera helix extract: crush Hedera helix, add it into a two-phase aqueous solvent at a solid-liquid ratio of 1:15 g / mL, wherein the two-phase aqueous solvent is a 50 wt % alcohol solution and a 20 wt % saline solution at a volume ratio of 1:3, extract at 70°C for 4 hours, and concentrate the extract under reduced pressure to obtain the Hedera helix extract;

[0063] Preparation of cucumber extract: cucumbers were repeatedly frozen and thawed and crushed, added into deionized water at a solid-liquid ratio of 1:30 g / mL, microwave extracted at 500 W and 80 °C for 5 h, and concentrated under reduced pressure to obtain cucumber extract;

[0064] Preparation of Salvia miltiorrhiza root extract: Cut the Salvia miltiorrhiza root into pieces and make it into pulp, add it to an alkaline solution with a pH value of 9.0 ± 0.5 and stir. The mass ratio of the Salvia miltiorrhiza root to the alkaline solution is 1:12, and treat it at 120 °C and 1.2 MPa for 10 min to obtain the Salvia miltiorrhiza root extract;

[0065] The preparation method of the above acne treatment composition containing ceramide specifically comprises the following steps: Subject the plant extract, ceramide, complex polysaccharide and penetration enhancer to high-pressure homogenization or microfluidization treatment for 40 min at a pressure of 1000 bar and a temperature of 50 °C, then add the gentiopicroside-vitamin C cocrystal and continue to stir for 20 min to obtain the acne treatment composition.

[0066] Comparative Example 1

[0067] The difference from Example 1 is that the amounts of raw materials are different, and the others are the same as those in Example 1.

[0068] That is, the acne treatment composition containing ceramide in this comparative example, by weight, comprises the following raw materials: 8 parts of plant extract, 4 parts of ceramide, 0.8 part of gentiopicroside-vitamin C cocrystal, 5 parts of complex polysaccharide, and 0.8 part of penetration enhancer.

[0069] Comparative Example 2

[0070] The difference from Example 1 is that the plant extract lacks Epimedium sagittatum extract, and the lacking amount is distributed according to the ratio of the other two extracts, and the others are the same as those in Example 1.

[0071] Comparative Example 3

[0072] The difference from Example 1 is that the gentiopicroside-vitamin C cocrystal is replaced with vitamin C, and the others are the same as those in Example 1.

[0073] Comparative Example 4

[0074] The difference from Example 1 is that the complex polysaccharide lacks Ganoderma lucidum polysaccharide, and the lacking amount is distributed according to the ratio of the other two polysaccharides, and the others are the same as those in Example 1.

[0075] Comparative Example 5

[0076] The difference from Example 1 is that the penetration enhancer is cucumber extract, and the others are the same as those in Example 1.

[0077] Comparative Example 6

[0078] The difference from Example 1 is that all plant extracts are simply extracted with alcohol, and the others are the same as those in Example 1.

[0079] Test Example 1 - Antibacterial Test

[0080] The antibacterial efficacy of the acne-combating compositions prepared in Examples 1-3 and Comparative Examples 1-6 against Propionibacterium acnes was tested;

[0081] Experimental method: Prepare a double-layer plate containing Propionibacterium acnes (ATCC11827). The lower layer of the double-layer plate is 15 mL of culture medium, and the upper layer is 5 mL of culture medium containing Propionibacterium acnes. The bacterial content in the plate is about 10 5 CFU / plate. Vertically place 2 Oxford cups on the surface of each plate. Take 200 μL of the acne-combating composition to be tested and put it into the Oxford cups, and anaerobically culture for 24 h at a temperature of 36 ± 1 °C. Then measure the diameter of the inhibition zone to evaluate the antibacterial efficacy against Propionibacterium acnes.

[0082] Evaluation criteria: High efficiency: inhibition zone diameter D ≥ 20 mm; Medium efficiency: inhibition zone diameter 15 ≤ D < 20 mm; Low efficiency: inhibition zone diameter 10 ≤ D < 15 mm; Ineffective: inhibition zone diameter D < 10 mm.

[0083] The experimental results are shown in Table 1.

[0084] Table 1

[0085]

[0086]

[0087] As can be seen from Table 1, the acne-combating compositions prepared in Examples 1-3 of the present invention have good effects in inhibiting Propionibacterium acnes. The antibacterial effects of Comparative Examples 1-6 all decreased to varying degrees. In particular, for Comparative Examples 2 and 6 where the formula and extraction method of the plant extract were adjusted, it can be seen that the antibacterial effect decreased significantly, indicating that the plant extract of the present invention has an important impact on the antibacterial efficacy. The plant extracts obtained by the three specific extraction methods cooperate with each other, which helps to improve the antibacterial effect and thus improve the acne-combating efficacy.

[0088] Test Example 2 - Anti-inflammatory test

[0089] Purchase 110 mice with a body weight of 100 ± 20 g and divide them into three groups, namely 10 mice in the blank control group, 10 mice in the model group, and the experimental group (Examples 1-3 group and Comparative Examples 1-6 group), with 10 mice in each group.

[0090] 24 hours before the experiment, remove the hair on the back area of 4 cm × 4 cm of all mice. On the first day, apply 100 μL of 1% DNCB to the back of the mice in the model group and the experimental group for sensitization. After that, apply 100 μL of 1% DNCB to the back test area every 3 days. On the 7th day, use 100 μL of 1% OX to strengthen the sensitization on the back test area. Continuously induce for 3 weeks according to the above process to prepare an atopic dermatitis-like mouse model; the blank control group is not treated with anything;

[0091] The experimental groups (Example 1 - 3 groups and Comparative Example 1 - 6 groups) were respectively given 100 μL of the anti - acne composition prepared in Examples 1 - 3 and Comparative Examples 1 - 6, evenly applied to the back test area, once a day, for 15 days of intervention. The blank group and the model group were given placebo, once a day, for 15 days of intervention.

[0092] Finally, the skin lesions in the back test area of the mice were collected, and the content of IL - 6 in the skin lesions was detected using an ELISA kit, and the inhibition rate of the inflammatory factor IL - 6 was calculated. The results are shown in Table 2.

[0093] Table 2

[0094] Group IL-6 Inhibition Rate / % Blank Control Group - Model Group - Example 1 78.8 Example 2 76.5 Example 3 77.1 Comparative Example 1 52.4 Comparative Example 2 30.7 Comparative Example 3 52.4 Comparative Example 4 55.9 Comparative Example 5 64.7 Comparative Example 6 25.6

[0095] As can be seen from Table 2, the anti - acne compositions prepared in Examples 1 - 3 of the present invention have a good effect of inhibiting the inflammatory factor IL - 6, can have anti - inflammation, thus improving the anti - acne efficacy, and the anti - inflammatory effects of Comparative Examples 1 - 6 all decreased to varying degrees.

[0096] Application Example

[0097] The mass percentage composition of the formula of the anti - acne cream: 5% of the anti - acne composition containing ceramide, 4% of glycerol, 2% of butanediol, 1% of glyceryl stearate, 0.1% of phenoxyethanol, and the balance is water.

[0098] The anti - acne cream containing 5 wt% was prepared according to the conventional preparation process.

[0099] Test Example 3 - Oil Control Test

[0100] 90 volunteers with relatively strong skin oil secretion, aged 18 - 35 years old, with half male and half female, were randomly divided into 9 groups, with 10 people in each group. The fixed position on the forehead of the subjects was selected as the experimental area. In an environment of 25 °C, the skin oil content of each subject before using the test sample and the skin oil content on the 14th day after using the test sample were measured using a CBS skin analysis system. The above - mentioned test sample was the anti - acne cream obtained from the anti - acne compositions of the examples and comparative examples through the formula and preparation method of the application example. The oil clearance rate was calculated, and the results are shown in Table 3.

[0101] Among them, the oil clearance rate = (skin oil content before using the test sample - skin oil content after using the test sample) / skin oil content before using the test sample × 100%

[0102] Table 3

[0103] Group Oil Removal Rate / % Example 1 82.5 Example 2 80.3 Example 3 81.1 Comparative Example 1 61.2 Comparative Example 2 42.6 Comparative Example 3 55.4 Comparative Example 4 52.5 Comparative Example 5 69.8 Comparative Example 6 46.7

[0104] As can be seen from Table 3, the acne-removing cream prepared from the acne-removing composition of the present invention has a good oil control effect, and the highest oil removal rate can reach 82.5%, thereby enhancing the acne-removing efficacy. Compared with Example 1, the oil control effects of Comparative Examples 1-6 are reduced to varying degrees.

[0105] Test Example 4 - Acne Test

[0106] Randomly select 90 subjects aged 18-35 years with skin problems such as acne, acne, enlarged pores, and oily shine, with 45 males and 45 females. Randomly divide them into 9 groups, namely Example 1-3 groups and Comparative Examples 1-6 groups, with 10 people in each group.

[0107] During the research period, the testers need to maintain a good living routine. The subjects use the acne-removing creams prepared from the acne-removing compositions of Examples 1-3 and Comparative Examples 1-6 through the formulations and methods of the application examples, once every two days, and leave them on the skin for 15 minutes each time. The usage period is 1 month. Record the skin oil control, water replenishment, visible acne changes and complications of the subjects using the products respectively.

[0108] Evaluate the skin conditions of the subjects according to clean and bright, reduced oiliness, acne cured, acne marks repaired and overall score. The evaluation method is: score each subject using the test product compared with the situation before use through sensory evaluation. The scoring standard is: 0-10 points, with 10 points being the best and 0 points being the worst.

[0109] The results are shown in Table 4.

[0110] Table 4

[0111]

[0112] As can be seen from Table 4, the acne-removing creams prepared from the acne-removing compositions of Examples 1-3 of the present invention have good acne-removing effects, can reduce skin oiliness and repair acne marks, and have good application prospects.

[0113] In addition, the acne-removing compositions prepared from Examples 1-3 and Comparative Examples 1-6 of the present invention were tested for irritation, and the results showed negative, indicating safety and no irritation.

[0114] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. An anti-acne composition containing ceramide, characterized in that: The composition comprises the following raw materials in parts by weight: 5-10 parts of plant extract, 1-3 parts of ceramide, 1-3 parts of gentiopicroside-vitamin C eutectic, 1-3 parts of complex polysaccharide, and 0.3-0.7 parts of transdermal enhancer; The plant extracts are Dendrobium officinale flower extract, Nymphaeaceae extract and Hedera helichrysum extract in a mass ratio of 1:1-3:2-4; The composite polysaccharide is tremella polysaccharide, tataric acid bean seed polysaccharide and ganoderma lucidum polysaccharide in a ratio of 1:3-5:0.5-0.8; The transdermal enhancer is cucumber extract and salvia miltiorrhiza root extract in a mass ratio of 1:1-3.

2. The acne-removing composition containing ceramide according to claim 1, characterized in that: The preparation method of the gentiopicroside-vitamin C eutectic comprises: adding gentiopicroside and vitamin C into n-hexane and stirring to form a suspension, continuously stirring for 24-48 hours, evaporating the solvent, and vacuum drying to obtain the gentiopicroside-vitamin C eutectic; The molar ratio of gentiopicroside to vitamin C is 1:2-4; the solid-liquid ratio of vitamin C to n-hexane is 1:30-40 g / mL.

3. The anti-acne composition containing ceramide according to claim 1, characterized in that: The preparation method of the butterfly dendrobium flower extract comprises: crushing and drying the butterfly dendrobium flower to form powder, adding the powder into a deep eutectic solvent, ultrasonically extracting, centrifuging, and concentrating under reduced pressure to obtain the butterfly dendrobium flower extract; The solid-liquid ratio of the butterfly Dendrobium flower and the deep eutectic solvent is 1:8-10g / mL; the deep eutectic solvent is prepared by adding ultrapure water to the hydrogen bond acceptor choline chloride and the hydrogen bond donor D-xylose in a molar ratio of 1:2, wherein the solid-liquid ratio of choline chloride to ultrapure water is 1:8-10mg / mL; the ultrasonic extraction is performed at 20-30kHz and 40-60°C for 4-6h.

4. The anti-acne composition containing ceramide according to claim 1, characterized in that: The preparation method of the Herba Lycopodii extract comprises: crushing the Herba Lycopodii, adding anhydrous ethanol for extraction, concentrating the extract under reduced pressure to form an extract, dispersing the extract with water, extracting with n-hexane, chloroform, ethyl acetate and n-butanol in sequence, retaining the chloroform phase and the ethyl acetate phase, concentrating under reduced pressure, and freeze-drying to obtain the Herba Lycopodii extract; The solid-liquid ratio of the three-branch nine-leaf grass to anhydrous ethanol is 1:10-12g / mL; the number of extractions is 2-3 times, each time for 40-60min, and the temperature is 50-70°C; the solid-liquid ratio of the extract to water is 1:6-8g / mL; the added amounts of n-hexane, ethyl acetate and n-butanol are all 5-7 times the weight of the extract; the number of extractions is 3 times, each time for 30-40min.

5. The anti-acne composition containing ceramide according to claim 1, characterized in that: The preparation method of the Hedera helix extract comprises: crushing the Hedera helix, adding the Hedera helix into a two-phase aqueous solvent, extracting, and concentrating the extract under reduced pressure to obtain the Hedera helix extract; The solid-liquid ratio of the rhombus-leaved ivy to the aqueous two-phase solvent is 1:10-15 g / mL; the aqueous two-phase solvent is a 40-50wt% alcohol solution and a 10-20wt% salt solution in a volume ratio of 1:1-3; the extraction temperature is 60-70°C and the extraction time is 2-4h.

6. The anti-acne composition containing ceramide according to claim 1, characterized in that: The preparation method of the cucumber extract comprises: repeatedly freezing and thawing the cucumber, crushing it, adding it into deionized water, performing microwave extraction, and concentrating it under reduced pressure to obtain the cucumber extract; The material-liquid ratio of the cucumber to deionized water is 1:20-30 g / mL; the microwave extraction is performed at 400-500 W and 60-80° C. for 3-5 hours.

7. The anti-acne composition containing ceramide according to claim 1, characterized in that: The preparation method of the salvia miltiorrhiza root extract comprises: cutting the salvia miltiorrhiza root into pieces and beating the pieces, adding the pieces into an alkaline solution with a pH value of 8.5-9.5, and stirring the solution to obtain the salvia miltiorrhiza root extract; The mass ratio of the salvia miltiorrhiza root to the alkaline solution is 1:8-12; the stirring treatment is carried out at 100-120° C. and 0.8-1.2 MPa for 5-10 minutes.

8. The method for preparing an anti-acne composition containing ceramide according to claim 1, characterized in that: The specific steps are: after the plant extract, ceramide, complex polysaccharide and transdermal enhancer are treated by high pressure homogenization or microfluidization, gentiopicroside-vitamin C eutectic is added and stirred continuously to obtain an anti-acne composition; The pressure of the high-pressure homogenization or microfluidization treatment is 800-1000 bar, the temperature is 40-50° C., and the time is 30-40 min; the stirring time is 10-20 min.

9. An acne-removing product, characterized in that: An anti-acne composition containing ceramide according to any one of claims 1 to 7.

10. An anti-acne product according to claim 9, characterized in that: The concentration of the anti-acne composition is 5-10 wt %.

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